JPH1194730A - Transparent lid for micro-plate with recessed surface - Google Patents
Transparent lid for micro-plate with recessed surfaceInfo
- Publication number
- JPH1194730A JPH1194730A JP29149597A JP29149597A JPH1194730A JP H1194730 A JPH1194730 A JP H1194730A JP 29149597 A JP29149597 A JP 29149597A JP 29149597 A JP29149597 A JP 29149597A JP H1194730 A JPH1194730 A JP H1194730A
- Authority
- JP
- Japan
- Prior art keywords
- sample
- micro
- plate
- microplate
- light
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Sampling And Sample Adjustment (AREA)
- Optical Measuring Cells (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Abstract
Description
【０００１】[0001]
【発明の利用分野】本発明は、医学・生物学領域で多用
されているマイクロプレート（マルチウェルプレート
等、名称多数）によるサンプル光学測定を改良するため
のものである。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention is to improve the optical measurement of a sample by using a microplate (multi-well plate, etc., many names) frequently used in the field of medicine and biology.
【０００２】[0002]
【従来の状況】近年、医学・生物学の領域においては、
複数の微量サンプル中の酵素活性や物質濃度等を測定す
る際、マイクロプレートを利用することが多い。マイク
ロプレートとは、2. Description of the Related Art In recent years, in the fields of medicine and biology,
A microplate is often used to measure enzyme activity, substance concentration, and the like in a plurality of trace samples. What is a microplate?
【図１】、FIG.
【図２】のごとく、複数のサンプル分注用ウェル（部
屋）（１）をもつ透明プラスチック製容器（２）で、マ
イクロプレート専用の光学測定器（マイクロプレートリ
ーダー）によって、一度に多数の微量サンプル（３）中
の酵素活性や物質濃度を測定することができる。マイク
ロプレートリーダーによる測定の原理は、As shown in FIG. 2, in a transparent plastic container (2) having a plurality of sample dispensing wells (rooms) (1), a large number of traces are measured at once by an optical measuring instrument (microplate reader) dedicated to a microplate. The enzyme activity and substance concentration in the sample (3) can be measured. The principle of measurement with a microplate reader is
【図３】のごとく、発色処理したサンプル（３）の入っ
たそれぞれのウェル（１）に特定波長の光（６）を通
し、サンプル（３）を通過してきた光量を検出器（７）
で測定し、その透過光量（吸光度）の違いによってそれ
ぞれのサンプル（３）中の酵素活性や物質濃度を知る、
というものである。この方式は、一度に多数の微量サン
プルを処理できるため、今後も使用頻度が高まること、
使用分野が広がることが予想される。As shown in FIG. 3, light (6) having a specific wavelength passes through each well (1) containing a color-processed sample (3), and the amount of light passing through the sample (3) is detected by a detector (7).
The enzyme activity and substance concentration in each sample (3) are known from the difference in the amount of transmitted light (absorbance).
That is. Since this method can process many small samples at once, it will be used more frequently in the future.
It is expected that the field of use will expand.
【０００３】[0003]
【本発明が解決しようとする課題】ところが従来の方式
には２つの問題点があった。第１に、正確な比較測定を
するためには、多数の微量サンプル（およそ５０マイク
ロリットルから数百マイクロリットル）（３）を厳密に
同量づつそれぞれのウェル（１）に分注しなければなら
ないことである。ウェル（１）中のサンプル（３）量が
異なると、サンプル溶液表面（４）が上下し、そのこと
が原因で通過する光量が変わってしまうため、正確な比
較測定ができなくなってしまう。しかし実際には、厳密
に同量のサンプル（３）を迅速に各ウェル（１）に分注
することは難しい。第２の問題点は、サンプル量が微量
のためHowever, the conventional method has two problems. First, in order to make accurate comparative measurements, a large number of microsamples (approximately 50 to several hundred microliters) (3) must be dispensed into each well (1) in exactly the same volume. It must not be. If the amount of the sample (3) in the well (1) is different, the surface of the sample solution (4) moves up and down, which changes the amount of light passing therethrough, so that accurate comparison measurement cannot be performed. However, in practice, it is difficult to quickly dispense the exact same amount of the sample (3) into each well (1). The second problem is that the sample volume is very small.
【図３】のごとく、水の表面張力によってサンプル溶液
表面（４）が平坦ではなくなることである。その為、マ
イクロプレートリーダー内の光源（５）軸が少しでもウ
ェル（１）の中心からずれると、透過光（６）がサンプ
ル溶液面（４）で屈折してしまい、正確な測定ができな
くなる。従って、マイクルプレートリーダー内の光源
（５）軸をそれぞれのウェル（１）の中心からずれない
ように厳密に調節することが重要となっている。本発明
は、これら２つの問題点を同時に解決するために為され
たものである。FIG. 3 shows that the surface of the sample solution (4) becomes uneven due to the surface tension of water. Therefore, if the axis of the light source (5) in the microplate reader is slightly deviated from the center of the well (1), the transmitted light (6) is refracted on the sample solution surface (4), and accurate measurement cannot be performed. . Therefore, it is important to precisely adjust the axis of the light source (5) in the microplate reader so as not to deviate from the center of each well (1). The present invention has been made to solve these two problems at the same time.
【０００４】[0004]
【図４】、FIG.
【図５】のごとく、マイクロプレートのウェルに対応す
るような複数の凹面部位（溝）（９）を有する透明プラ
スチック製の蓋（８）を発明した。なお、現在マイクロ
プレートは、サンプル分注用のウェル（１）数が、６ウ
ェル、１２ウェル、２４ウェル、９８ウェルのものが市
販されているので、本発明（８）凹面部位（９）の形
状、数はそれぞれのマイクロプレートに対応したものと
する。As shown in FIG. 5, a transparent plastic lid (8) having a plurality of concave portions (grooves) (9) corresponding to the wells of the microplate was invented. At present, microplates having 6 wells, 12 wells, 24 wells and 98 wells for dispensing sample (1) are commercially available. The shape and number correspond to each microplate.
【０００５】[0005]
【図６】のごとく、本発明、凹面付きマイクロプレート
用透明蓋（８）を、サンプル（３）を分注したマイクロ
プレート（２）に装着する。As shown in FIG. 6, the transparent lid (8) for a microplate with a concave surface according to the present invention is attached to the microplate (2) into which the sample (3) has been dispensed.
【図７】のごとく、マイクロプレートリーダーを使っ
て、サンプル（３）中の酵素活性や物質濃度の光学測定
を行う。光源（５）から放射された特定波長光（６）
は、ウェル（１）底面、サンプル溶液（３）、本発明凹
面部（９）底面を通り、光検出器（７）に達する。As shown in FIG. 7, an optical measurement of the enzyme activity and the substance concentration in the sample (3) is performed using a microplate reader. Specific wavelength light (6) emitted from the light source (5)
Passes through the bottom of the well (1), the sample solution (3), the bottom of the concave portion (9) of the present invention, and reaches the photodetector (7).
【０００６】[0006]
【発明の効果】本発明によって、従来の２つの問題点が
解決される。第１の問題点であった「マイクロプレート
ウェルに分注するサンプル量が異なると、光学測定時の
障害となる」という点に関しては、光学測定時に各サン
プル（３）を通過する光の通過距離が、本発明の凹面部
位（９）底面とマイクロプレートウェル（１）底面によ
って同一距離に統一されるため、解決できるAccording to the present invention, two conventional problems are solved. Regarding the first problem, that "different amounts of sample to be dispensed into microplate wells hinder optical measurement", the passage distance of light passing through each sample (3) during optical measurement Can be solved because the concave portion (9) bottom surface of the present invention and the microplate well (1) bottom surface are unified at the same distance.
【図７】。即ち、各ウェル（１）中のサンプル（３）量
が多少異なっても、本発明によって、光（６）の通過距
離は常に一定となり、正確な光学測定が可能となる。ま
た、第２の問題点であった測定時の光の屈折の問題も、FIG. That is, even if the amount of the sample (3) in each well (1) is slightly different, the present invention makes the passing distance of the light (6) always constant, thereby enabling accurate optical measurement. In addition, the second problem of refraction of light at the time of measurement,
【図７】のごとく、本発明凹面部位（９）の平坦底面が
サンプル溶液（３）中に浸水するため、測定光（６）は
屈折することなく検出器（７）に達することになり、解
決される。なお、本発明の凹面部位（９）をサンプル溶
液（３）に浸す際に気泡が発生、付着し、光学測定の障
害となる可能性があるが、その際は、マイクロプレート
（２）を指で弾く等して気泡を除去できるが、As shown in FIG. 7, since the flat bottom surface of the concave portion (9) of the present invention is immersed in the sample solution (3), the measurement light (6) reaches the detector (7) without being refracted. Will be resolved. In addition, when the concave portion (9) of the present invention is immersed in the sample solution (3), bubbles may be generated and attached, which may obstruct the optical measurement. You can remove bubbles by playing with
【図７】のごとく、本発明の凹面部位（９）底面の角の
面取り（１０）を施したものを用いることによって、気
泡除去が容易になる。As shown in FIG. 7, the use of the concave portion (9) with the chamfer (10) of the bottom surface of the present invention facilitates the removal of air bubbles.
【図１】 従来のマイクロプレート平面図（模式図）FIG. 1 is a plan view (schematic diagram) of a conventional microplate.
【図２】 Ａ−Ａ線断面図FIG. 2 is a sectional view taken along line AA.
【図３】 Ｂ−Ｂ線拡大断面図（光学測定実施例模式
図）FIG. 3 is an enlarged cross-sectional view taken along line BB (a schematic diagram of an optical measurement example).
【図４】 本発明凹面付きマイクロプレート用透明蓋平
面図（模式図）FIG. 4 is a plan view (schematic diagram) of a transparent lid for a microplate with a concave surface according to the present invention.
【図５】 Ｃ−Ｃ線断面図FIG. 5 is a sectional view taken along line CC.
【図６】 本発明凹面付きマイクロプレート用透明蓋の
実施例の側面断面図FIG. 6 is a side sectional view of an embodiment of the transparent lid for a microplate with a concave surface according to the present invention.
【図７】 本発明一部拡大断面図（光学測定実施例模式
図）FIG. 7 is a partially enlarged cross-sectional view of the present invention (a schematic diagram of an optical measurement example).
１：ウェル ２：マイクロプレート本体 ３：サンプル
溶液 ４：サンプル溶液表面 ５：光源 ６：光 ７：
光検出器 ８：凹面付きマイクロプレート用透明蓋
９：凹面（溝） １０：面取り部位1: Well 2: Microplate body 3: Sample solution 4: Sample solution surface 5: Light source 6: Light 7:
Photodetector 8: Transparent lid for concave microplate
9: concave surface (groove) 10: chamfered part
Claims (2)
複数の凹面部位（溝）（９）を有する透明プラスチック
製の蓋（８）。1. A transparent plastic lid (8) having a plurality of concave portions (grooves) (9) for mounting on a microplate (2).
（１０）を施した請求項１の凹面付きマイクロプレート
用透明蓋。2. The transparent lid for a microplate with a concave surface according to claim 1, wherein the concave portion (9) is chamfered (10) at the corner of the bottom surface as shown in FIG.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP29149597A JPH1194730A (en) | 1997-09-17 | 1997-09-17 | Transparent lid for micro-plate with recessed surface |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP29149597A JPH1194730A (en) | 1997-09-17 | 1997-09-17 | Transparent lid for micro-plate with recessed surface |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH1194730A true JPH1194730A (en) | 1999-04-09 |
Family
ID=17769621
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP29149597A Pending JPH1194730A (en) | 1997-09-17 | 1997-09-17 | Transparent lid for micro-plate with recessed surface |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH1194730A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2006214986A (en) * | 2005-02-07 | 2006-08-17 | Yamato Scient Co Ltd | Sample container |
| JP2009052893A (en) * | 2007-08-23 | 2009-03-12 | Otsuka Denshi Co Ltd | Solution characteristic measuring instrument and solution characteristic measuring method |
| JP2017508956A (en) * | 2014-02-10 | 2017-03-30 | ナノバイオシス インコーポレーテッドＮａｎｏｂｉｏｓｙｓ Ｉｎｃ． | Microfluidic chip and real-time analyzer using the same |
| WO2020144754A1 (en) * | 2019-01-09 | 2020-07-16 | 株式会社日立ハイテク | Size distribution measurement device, size distribution measurement method, and sample container |
| WO2023139777A1 (en) * | 2022-01-24 | 2023-07-27 | 株式会社日立ハイテク | Particle measurement device, particle measurement method, sample container |
-
1997
- 1997-09-17 JP JP29149597A patent/JPH1194730A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2006214986A (en) * | 2005-02-07 | 2006-08-17 | Yamato Scient Co Ltd | Sample container |
| JP2009052893A (en) * | 2007-08-23 | 2009-03-12 | Otsuka Denshi Co Ltd | Solution characteristic measuring instrument and solution characteristic measuring method |
| JP2017508956A (en) * | 2014-02-10 | 2017-03-30 | ナノバイオシス インコーポレーテッドＮａｎｏｂｉｏｓｙｓ Ｉｎｃ． | Microfluidic chip and real-time analyzer using the same |
| WO2020144754A1 (en) * | 2019-01-09 | 2020-07-16 | 株式会社日立ハイテク | Size distribution measurement device, size distribution measurement method, and sample container |
| CN113227759A (en) * | 2019-01-09 | 2021-08-06 | 日本株式会社日立高新技术科学 | Size distribution measuring device, size distribution measuring method, and sample container |
| WO2023139777A1 (en) * | 2022-01-24 | 2023-07-27 | 株式会社日立ハイテク | Particle measurement device, particle measurement method, sample container |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A621 | Written request for application examination |
Effective date: 20040827 Free format text: JAPANESE INTERMEDIATE CODE: A621 |
|
| A131 | Notification of reasons for refusal |
Effective date: 20060228 Free format text: JAPANESE INTERMEDIATE CODE: A131 |
|
| A02 | Decision of refusal |
Effective date: 20060704 Free format text: JAPANESE INTERMEDIATE CODE: A02 |