JPH11299495A - Test kit - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide water-diffusive sheet in which the base materials are stuck to each other, as the nonwoven fabric retains its water diffusivity in the test kit equipped with a water-diffusive sheet. SOLUTION: In the test kit that has a sheet having a plurality of mutually separated water absorption parts 1 for reaction of the test solution and a water- diffusive sheet 2 that at least partially contacts with the water-absorbing part for supplying the test solution to the water absorption part, the water-diffusive sheet 2 is prepared by sticking the nonwoven fabric to the base material with a thermoplastic resin. Since the water-diffusive sheet 2 has excellent water diffusion, the test can be simply and promptly performed.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

The present invention makes it possible to carry out chemical and microbiological tests requiring a large variety of reactivity more easily and quickly than conventional microplates.
In addition, the present invention relates to a test tool that can be industrially advantageously mass-produced.

[0002]

2. Description of the Related Art In a chemical test including a step of reacting a chemical sample with some kind of reagent and a microbiological test including a step of culturing a microorganism in the sample, particularly, treatment of a large number of samples, In a test requiring a reaction with a reagent or culture in a large number of systems, a plastic microplate provided with a plurality of wells is usually used as a reaction or culture vessel.

In a test using such a microplate, individual reactions, culture of microorganisms, and the like are performed in each well on the plate. For example, M by a microfluidic dilution method using a microplate
In IC measurement, various concentrations of antimicrobial agents are added to a plurality of wells, and the growth of the target bacterium in each well is observed to determine the minimum growth inhibitory concentration.

[0004] As a test tool used for such a test, for example, a product in which a predetermined amount of an antibacterial agent is previously dispensed into each well of a microplate, which has been dried, and which has been frozen and stored as it is, has been commercialized. However, such a test device has a problem in that the cost of the microplate itself is high, so that it becomes an expensive product, and a reduction in price has been desired. In addition, even when a large number of tests are performed with one type of sample, a predetermined amount of the sample must be dropped in each well, so that there is a problem that the operation is complicated.

[0005] Therefore, a sheet provided with a plurality of mutually isolated water absorbing portions for reacting the test solution is provided at least partially in contact with the water absorbing portion to supply the test solution to the water absorbing portion. It has been found that the use of a combination of the water-diffusible sheets described above makes it possible to perform a multi-item test by dropping the test liquid onto one diffusion part.

As the water diffusing sheet used here, a non-woven fabric having high diffusivity and low water absorption is preferably used.
Since this product has a low basis weight and is very difficult to handle, it was necessary to bond and support a substrate. Conventionally, this bonding has been performed by applying an adhesive to a base material and bonding a nonwoven fabric.

[0007]

However, when laminating with an adhesive, the nonwoven fabric immediately after lamination shows good diffusibility, but there is a problem that after several days the diffusivity decreases.

[0008] That is, after the adhesive is applied to the base material, the adhesive cures when the solvent is removed by a drier, but at this point, the adhesive has not been completely polymerized yet. When a nonwoven fabric is pasted here, the adhesive component before polymerization is caused by capillary action,
Since the nonwoven fabric enters the eyes of the nonwoven fabric and polymerizes with time, there is a problem that a nonwoven fabric having no thickness loses its diffusivity and absorbency.

Accordingly, it is an object of the present invention to provide a water-diffusing sheet having a water-diffusing sheet and a substrate bonded thereto while maintaining the water-diffusing property of the nonwoven fabric used.

[0010]

Means for Solving the Problems In view of such a situation, the present inventors have conducted intensive studies. As a result, when the nonwoven fabric and the base material are bonded with a thermoplastic resin, the water diffusibility is maintained. They found that a sheet was obtained and completed the present invention.

That is, according to the present invention, there is provided a sheet provided with a plurality of mutually isolated water absorbing portions for reacting a test solution and supplying the test solution to the water absorbing portion by at least partially contacting the water absorbing portion. The present invention provides a test device having a water-diffusible sheet for performing the test, wherein the water-diffusible sheet is a nonwoven fabric and a substrate bonded to each other with a thermoplastic resin. .

[0012]

BEST MODE FOR CARRYING OUT THE INVENTION The material of the non-woven fabric of the water-diffusing sheet used in the present invention includes, for example, rayon, polyester, acrylic, vinylon, cotton, glass fiber, and fibers made of other chemical and natural materials. Among them, cellulosic nonwoven fabrics such as cotton having high diffusivity and low water absorption are preferred.

Further, the nonwoven fabric has a water absorption of 0.005 to 0.0
5 g / cm ^2, particularly preferably has a 0.008~0.02g / cm ^2, also when immersed one end of the short sides of the rectangular sheet of 1 cm × 10 cm in purified water within 15 minutes, in particular 30 It is preferable to use a material having a water diffusion rate at which water diffuses over the entire surface in seconds to three minutes. The water absorption is in the same range even after being attached to the substrate.

The amount of water absorption used in the present invention is:
Crow et al. (INDA-TEC, 207 (199)
The value obtained by partially modifying 1)) according to the following method. 1) A sponge (polyurethane) having a size of 8.5 cm × 14 cm and a thickness of 3 cm is sufficiently hydrated in advance and immersed in a dish containing purified water. 2) A 1 cm × 1 cm test piece is placed on a water-containing sponge, and the same type of sponge is stacked. 3) Put the whole dish in a wet box and place at room temperature (20-25 ° C) for 5 minutes.
After standing for a period of time, the sample weight is measured. 4) The value obtained by subtracting the sample weight before water absorption from the sample weight after water absorption is defined as the water absorption.

The diffusion sheet used in the present invention is for supplying a test liquid and diffusing it to a water absorbing portion.
The shape, size, and thickness of such a diffusion sheet are not particularly limited, and can be selected according to the amount of water absorbed in the water absorbing portion, the number of water absorbing portions, and the like of the amount of the test liquid to be used. Usually, a sheet-like material is preferable, and the thickness of the nonwoven fabric is 0.05 to
Those having a thickness of about 0.6 mm are preferably used. When the thickness of the nonwoven fabric exceeds 0.6 mm, there is not much difference in diffusibility even when an adhesive is used for bonding to the base material.

The substrate to be bonded to the nonwoven fabric is not particularly limited, and examples thereof include plastic sheets such as polyester, polypropylene, polyethylene, polystyrene, polycarbonate, and polyvinyl chloride, paper and synthetic paper. A polyester sheet is preferred from the viewpoint. The thickness of the substrate is not particularly limited and may be appropriately selected depending on the intended purpose; however, it is usually preferably about 9 to 1000 μm, and particularly preferably about 25 to 200 μm.

The thermoplastic resin used for bonding in the present invention is not particularly limited as long as it is lower than the melting point of the substrate. Examples thereof include polyolefins such as polyethylene, polypropylene and vinyl acetate; polyesters, polyacrylates and natural rubbers. Resin. In the present invention,
As a method of laminating the base material and the nonwoven fabric, a method of melt-extruding a thermoplastic resin between the nonwoven fabric and the base material (hereinafter, referred to as “melt extrusion method”) or providing a thermoplastic resin layer on the base material, A method of laminating a nonwoven fabric on the resin layer side and performing thermal lamination (hereinafter, referred to as “thermal lamination method”) may be used.

In the above-mentioned melt extraction method, it is preferable that the thermoplastic resin is polyethylene, polypropylene, vinyl acetate or the like suitable for extrusion.

In the thermal lamination method, for example, the following method is provided as a method of providing a thermoplastic resin layer on a substrate. (1) A method of applying a thermoplastic resin on a substrate by a melt extrusion method. (2) A method of coating a base material with a thermoplastic resin (heat sealing agent). (3) A method of attaching a thermoplastic film to a substrate by a dry lamination method.

As the thermoplastic resin used in the method (1), those exemplified by the melt extrusion method are preferable. As the heat sealant used in the method (2), polyester-based, polyacrylate-based and natural rubber are used. The film used in the method (3) is preferably a thermosetting film of polyamide, polyolefin, polyester or the like.

In the present invention, one or more kinds of thermoplastic resins may be used, and when two or more kinds are used,
It may be a multilayer. Further, a thermoplastic resin layer may be provided after applying an anchor agent to the base material in advance.

The bonding method by the thermal lamination method is performed by a known method such as passing through a hot roll or pressing with a hot plate. It is also possible to pattern a roll or a flat plate to obtain a desired pattern on the nonwoven fabric side. At this time,
By increasing the pressure and temperature applied by a hot roll or hot plate, the nonwoven fabric can be buried in a thermoplastic resin to draw a design.

In the test device of the present invention, as a material of a water-absorbing sheet used for a sheet provided with a plurality of mutually isolated water-absorbing portions for reacting a test solution, for example,
Examples thereof include rayon, polyester, acrylic, vinylon, cotton, glass fiber, and fibers made of other chemical and natural materials. Among them, an acrylic nonwoven fabric is preferable because of its high water absorption. The water-absorbing sheet preferably has a water absorption of 0.020 g / cm ² or more, preferably 0.08 to 0.2 g / cm ² .

The water-absorbent sheet has a thickness of 300 to 3
000 μm is preferable, and it is particularly preferable to use one having a thickness of 500 to 2000 μm. If the thickness is less than 300 μm, the amount of the test liquid held in the water absorbing portion is not sufficient, and the reaction is not sufficient. As a result, accurate measurement may not be performed. On the other hand, when the thickness exceeds 3000 μm, it becomes difficult to perform the hydrophobic treatment described below.

The test device of the present invention is obtained, for example, by subjecting only the portions other than the water absorbing portions to a hydrophobic treatment so that a plurality of mutually isolated water absorbing portions are formed on the water absorbing sheet. Can be

The hydrophobizing treatment is preferably carried out by a printing method, that is, by permeating a liquid containing a hydrophobic resin as a main component into a predetermined portion (a portion other than the water absorbing portion) of the water absorbent sheet and then drying. . Examples of the hydrophobic resin used here include a polyurethane resin, a polyacrylic resin, a polyester resin, a polyamide resin, a polyvinyl chloride resin, a polystyrene resin, a fluorine resin, and other rubber modified products. As long as the resin is not particularly a water-soluble resin or a resin having a high hygroscopicity, any known resin conventionally used as a binder of an ink can be used. In addition, the solvent for dissolving or dispersing these hydrophobic resins differs depending on the hydrophobic resin used, but is selected from various organic solvents and used, for example,
Examples include toluene and cyclohexanone. In addition, a liquid containing a hydrophobic resin as a main component (hydrophobic ink) may include a coloring agent, an inorganic or organic filler, a water repellent such as a fluororesin or silicone, a surfactant, and a viscosity adjusting agent, if necessary. Additives such as agents can be added.

In order to make the hydrophobic ink permeate a predetermined portion of the water-absorbent sheet, it is preferable to use a usual silk screen printing method. That is, it is preferable that the hydrophobic ink is applied to a predetermined portion of the water-absorbent sheet, penetrated, and then dried.

In order for the hydrophobic ink thus applied to sufficiently penetrate into the back surface of the water-absorbent sheet, (1) a method of applying pressure to the coated surface, and (2) a method of sucking from the surface opposite to the coated surface. , (3) a method of blowing air from the application surface side, and (4) a method of appropriately combining two or more of these (1), (2) and (3).

(1) The method for applying pressure to the application surface is not particularly limited, but a roll press method is a preferable method.

In this method, for example, at least two mirror-finished metal rolls and rubber rolls are arranged in a vertical combination, and a water-absorbent sheet coated with hydrophobic ink is placed between the rolls with the coated surface facing the metal roll. It is a method of inserting and rotating both rolls while continuously pressing between them. At this time, the mirror-finished metal roll can be heated, but when the hydrophobic ink is not dried, the hydrophobic ink can be permeated to the back surface of the hydrophobic sheet only by pressing without heating. Can be. According to this method, it is not necessary to prepare a mold or the like having a predetermined shape for pressing, and pressurization can be easily performed with high productivity only by adjusting the rotation speed of the rolls and the gap or nip pressure between the rolls. . Further, when pressurizing, it is preferable that a film that does not absorb the hydrophobic ink is overlaid on the application surface on the water-absorbent sheet, and an absorbent material such as paper is overlaid on the surface on the opposite side of the application surface. By adopting such a method, excess ink that has permeated to the back surface by pressure can be absorbed by the absorbent material, and the adhesion of the ink to the roll and the spread of the water-absorbent sheet in the lateral direction can be reduced. Can be prevented.

As another method of applying pressure to the ink-coated surface of the water-absorbent sheet, there is a flat-press method using a flat plate at normal temperature or heated or a flat plate patterned into a shape corresponding to the application pattern of the hydrophobic ink. be able to. When the flat press method is employed, when the ink applied to the water-absorbent sheet is not dried, the pressure plate may be at room temperature as in the case of the roll press method, but the pressure is applied after drying the ink. In this case, it is necessary to heat the pressure plate in order to soften or melt the hydrophobic ink.

In the case of applying pressure using a patterned pressure plate, the pressure plate and the application pattern of the water-absorbent sheet are, of course, positioned and pressed.

Also in this method, when the hydrophobic ink on the water-absorbent sheet is not dried at the time of pressurizing, a film which does not absorb the hydrophobic ink is overlaid on the coated surface, and the surface opposite to the coated surface is also applied. In this case, it is preferable that an ink-absorbing material such as paper is overlaid and pressed. Also, if the applied ink is dried and then pressed with a heated flat plate, heat-resistance is applied to both to prevent the softened or melted hydrophobic ink from sticking to the pressed flat plate and the receiving surface plate. It is preferable to perform a release treatment.

In the method (2) of sucking from the surface opposite to the coating surface, when the coating means is a silk screen printing system, a vacuum suction device for fixing the sheet to be printed on the stage on the printing machine itself. This is used as it is, and if necessary, by adjusting the hole diameter of suction holes and their intervals or their arrangement pattern, suction can be performed simultaneously with printing and ink can permeate.
Also, if the printing machine does not have a vacuum suction device,
Separately, a similar suction device can be prepared so that the ink is sucked and permeated before printing before the ink dries.

In the case of the method (3), in which the pressure is applied by blowing air from the coating surface side and the water is absorbed, a blower with a hood and a wire net-like pedestal are prepared, and the coated water-absorbent sheet is prepared. The ink can be permeated by placing it on a receiving table and applying wind pressure from above the application surface with a blower. In addition to the above, there is an air pressure / vacuum forming machine for a plastic sheet as a ready-made device, but a chamber for this forming can also be used. In this case, a wire net-shaped pedestal is prepared separately, this is replaced with a female mold for molding, installed in the chamber, a coated water-absorbing sheet is placed thereon, and after closing the chamber, no vacuum is used. The ink can be penetrated by sending only air for pressurized air from above the application surface and pressurizing only by exhausting.

In the case of a combination of (2) and (3), that is, a method of performing both blowing from the application surface side and suction from the surface opposite to the application surface, the method of (3) is used. The molding chamber of the pressurized / vacuum molding machine described can be used as it is. Also in this case, instead of a female mold for molding, a wire net-shaped pedestal is mounted in the chamber, a coated water-absorbent sheet is placed on it, and after closing the chamber, pressure is applied from above the application surface by compressed air. And ink is allowed to penetrate by performing suction from the lower side by vacuum.

Usually, in the pressurizing / vacuum forming machine, a heating device and a forming device for the plastic sheet are lined up. However, in the above-mentioned methods (2) and (3), heating is not required. It is also possible to separately prepare a device, connect the device after the silk screen printing machine, and if necessary, further connect a drying device thereafter, so that the application, the permeation, and the drying can be continuously performed.

The shape and size of the water-absorbing portion left by the above-mentioned hydrophobic treatment are not particularly limited, and can be selected according to the amount of the test solution, the amount of water absorbed, and the like. Usually, a circular or square shape is preferable, and for example, a shape having a diameter or a side length of about 0.2 to 1 cm is preferably used. In this case, the amount of the test liquid to be absorbed by one water absorbing portion is generally about 0.025 to 0.2 ml.

The number of such water absorbing portions is not particularly limited as long as it is plural, and may be arbitrarily determined from the amount of the test liquid, the number of test items, and the like.

In order to adjust the amount of water absorbed by the water absorbing section,
A material having the water-absorbing polymer fixed thereon can be used as the water-absorbing portion. As the water-absorbing polymer used here,
For example, soluble starch, mannan, agar, sodium alginate, plant mucilage (such as gum arabic), microbial mucilage (dextran, xanthan gum, gellan gum, etc.), cellulosic substances (methyl cellulose, carboxymethyl cellulose, other cellulose), polyvinyl alcohol, Examples thereof include sodium polyacrylate and a graft polymer of these polymers. As a method of fixing these water-absorbing polymers to the water-absorbing member, for example, a water-absorbing polymer is dissolved or emulsified in water, an organic solvent, or the like, a printed water-absorbing sheet is immersed, and then dried and fixed. And a method in which the water-absorbing polymer liquid is dropped onto the water-absorbing section and then dried and fixed.

The test device of the present invention comprises the above-mentioned water-absorbing sheet and a diffusion sheet for contacting at least a part of the water-absorbing portion and supplying a test liquid to the water-absorbing portion.
The ratio of the amount of water absorption between the water absorbing portion and the diffusion portion (water absorbing portion / diffusion sheet) is preferably 2 or more, particularly preferably 5 to 15. If the ratio is less than 2, it may be difficult to diffuse the test liquid into the diffusion sheet and cause the water absorbing section to absorb water.

In the test device of the present invention, the water-absorbing portion of the sheet-shaped test device is arranged so as to be at least partially in contact with the diffusion sheet.

The method for fixing the water-absorbing portion of the sheet-shaped test device to the diffusion sheet is not particularly limited. A method of fixing with water; a method of placing a water-absorbing portion on the diffusion sheet and bonding a part by (heat) crimping; a method of sewing the diffusion sheet and the water-absorbing portion; There is a method of fitting.

In a more preferred embodiment of the test device of the present invention, the water-absorbent sheet has a test liquid supply hole at the center of the plurality of water-absorbing portions, and a water-diffusible sheet is used.
It is placed on the upper side of the diffusion sheet so as to connect the test liquid supply hole and the plurality of water absorbing portions (see FIG. 1).

In order to conduct a test using the sheet-shaped test device of the present invention, a predetermined amount of a test liquid may be dropped on each water absorbing portion. The amount of the test liquid is determined according to the size of the water absorption part, the amount of water absorption, and the like.

In order to conduct a test using the test device of the present invention, a predetermined amount of the test liquid may be poured into any one of the diffusion sheets, whereby the test liquid diffuses through the diffusion sheet. Then, water is absorbed by each of the plurality of water absorbing portions. The amount of the test liquid is determined according to the size of the diffusion sheet and the water absorbing portion, the amount of water absorption, and the like.

In the present invention, the test liquid absorbed in each water absorbing section reacts with various reaction reagents and the like. The reaction reagent or the like may be contained in the test device in advance, or may be added after water absorption. If there is no problem in stability or the like, it is preferable to include the reaction reagent in advance for performing a rapid test. preferable. The reaction reagents used here are not particularly limited, and are, for example, reagents for measuring enzyme activities such as various chromogenic substrates and fluorescent substrates; reagents for identifying microorganisms of sugars, amino acids and fatty acid sugars; and measuring drug sensitivity to microorganisms such as various antibiotics. Reagents;
Antimicrobial test reagents for various antibacterial substances and the like can be mentioned. These reaction reagents can be fixed, for example, by adding a reagent solution to a previously sterilized water-absorbing part so as to have a water absorption per unit area or less, and then drying under reduced pressure.
At this time, the above-described water-absorbing polymer may be added together with the reaction reagent.

[0048] Reactive reagents of different types and concentrations can be immobilized on the plurality of water-absorbing parts of the test device of the present invention by such a method, and a multi-item reaction performance can be tested at a time. The reaction reagent at this time is, for example, a test solution (a liquid such as a bacterial suspension, a body fluid, a cerebrospinal fluid, or a food emulsion).
L-alanine used for enzyme activity measurement
p-nitroanilide and L-proline
For chromogenic substrates such as -β-naphthylamide,
The amount of the test solution absorbed in the water-absorbing piece is 0.01 to 0.1%.
5% by weight; 4-methylumbelliferyl
-Β-D-galactopyranoside, L
-Alanine-7-amido-4-methyll
In the case of a fluorescent substrate such as coumarin, it is preferable to use the fluorescent substrate in the range of 0.001 to 0.02% by weight based on the amount of the test liquid absorbed in the water absorbing piece. In the case of sugars, amino acids, fatty acids or their intermediate metabolites used for the microorganism identification test, the amount of the test liquid absorbed by the water absorption part is set at 0.
In the case of an antibiotic used for measuring drug sensitivity to microorganisms or an antibacterial substance used for an antibacterial test, the drug concentration can be arbitrarily set depending on the target drug and the target microorganism species. it can.

The water-absorbing part may contain a gelling agent so as to increase the viscosity of the test liquid having absorbed water and to obtain sufficient gel strength in the water-absorbing part. Examples of such gelling agents include natural polymers such as xanthan gum, carrageenan, gellan gum, gum arabic, locust bean gum, guar gum, tragacanth gum, and crystalline cellulose;
Examples include synthetic polymers such as polyvinylpyrrolidone, polyvinyl alcohol, polyethylene glycol, polypropylene glycol, polyacrylamide, polyacrylic acid, hydroxyethyl cellulose, and hydroxypropyl cellulose. These gelling agents can be fixed to the water-absorbing part in the same manner as for fixing the above-mentioned reagent. The gelling agent at this time is 0.1 to 1% by weight for a natural polymer and 0.05 to 0. 0% for a synthetic polymer, based on the amount of the test liquid absorbed by the water absorbing portion. Preferably, it is used in the range of 5% by weight.

Further, when the above-mentioned reaction reagent or gelling agent is fixed to the water-absorbing part, various nutrients such as peptone and gel strength are increased for culturing bacteria in the test solution, and the reaction sensitivity is improved. For this purpose, a cation such as an alkali metal salt or an alkaline earth metal salt is blended in the reagent solution, so that these can be contained in the water absorbing portion.

Further, various nutrients, surfactants, cations such as alkali metal salts and the like can be mixed in the test solution.

[0052]

EXAMPLES The present invention will be further described with reference to examples, but the present invention is not limited to these examples.

Production Example A water diffusible sheet was produced by the following method using the following materials. (Production Example 1) (Material) Cellulosic nonwoven fabric (PS-140, manufactured by Asahi Kasei Corporation)
0.08mm thickness polyethylene terephthalate (PET) film thickness 7
5 μm (manufactured by Unitika) Linear low-density polyethylene (LLDPE) resin (Ultzex 15101C Mitsui Petrochemical) (Method) A 75 μm PET film is used as the primary paper feed, and a polyester anchor agent (Toyo Morton EL)
-510) was applied 1.0 μm, and a polyester-based nonwoven fabric was extruded and laminated using an LLDPE resin to prepare a laminated film having the following structure. PET75μm / anchor coat / LLDPE15μm
/ Non-woven fabric This sheet was punched out to a size of 15 × 110 mm to obtain a developed layer sample.

(Production Example 2) (Material) Cellulosic nonwoven fabric (PS-140 manufactured by Asahi Kasei Corporation)
0.08 mm thickness PET film 75 μm (made by Unitika) Polyolefin resin (VMX Mitsubishi Chemical Co., Ltd.) (Method) A 75 μm PET film is used as primary paper, and a polyester anchor (Toyo Morton EL-
After 510) was applied by 1.0 μm, VMX was extrusion-coated to obtain a laminated film having the following structure. PET 75 μm / anchor coat / VMX 10 μm A cellulosic nonwoven fabric is laminated on the VMX side of this laminate, and the roll temperature is 8 with a laminating machine (TORAMI).
Bonded at 0 ° C. This sheet was punched out to a size of 15 × 110 mm to obtain a spread layer sample.

(Comparative Example 1) (Material) Cellulosic nonwoven fabric (PS-140 manufactured by Asahi Kasei Kogyo Co., Ltd.) PET film 75 μm Adhesive (Takelac A-511) Hardener (Takelac A-50) Mixing ratio: A-511: A -50: ethyl acetate = 10:
1: 5

(Method) An adhesive was applied to a PET film using a No. 9 bar. After the solvent was removed by a drier, a nonwoven fabric was stuck with a rubber roller to obtain a sample. This sheet was punched out to a size of 15 × 110 mm to obtain a spread layer sample.

(Production Example 3) Production method of sheet provided with water absorbing portion (Material) Acrylic non-woven fabric (A-200 made by Kuraray Trading) thickness 1.2 mm Ink Acrylic resin (Sericol 13 Medium Teikoku Ink Production) 80 Parts by weight Solvent (Sericol G solvent manufactured by Teikoku Ink Manufacturing)
10 parts by weight Fluoropolymer (comb-shaped polymer LFB-4015
10 parts by weight (manufactured by Soken Kagaku) (Printing method) Plate Number of lines 70 mesh Resist thickness 90μ Printing Print once by a known screen printing method (Roll press) Print, then print side up and OP upward
The P film and the wiper were overlapped on the lower side and roll-pressed to infiltrate the ink. By the above-mentioned method, 12 pieces of 7.5 mm circle non-printed whole printed pieces having a non-printed part were prepared, and the printed pieces were punched into a rectangular piece of 106 × 38 mm, and the central part was formed into a 13 mm × 18 mm elliptical shape. Punched out.

Examples 1 and 2 The sheets provided with the water absorbing portions of Production Example 3 were laminated on the water diffusible sheets of Production Examples 1 and 2 and Comparative Example 1, respectively, as shown in FIG. Was manufactured. This was stored in an environment at 37 ° C. and a humidity of 30%, and evaluated by the following evaluation method. Table 1 shows the results. Evaluation method 1 ml of pure water was dropped from the center, and after 1 minute, it was tested whether it developed in 12 holes of the water absorption part and confirmed visually.

[0059]

[Table 1]

[0060]

According to the test device of the present invention, since a sheet having excellent water diffusibility is used, it is not necessary to inject a test solution into each well unlike a conventional microplate, and the test device is coated at one place of the diffusion sheet. By pouring the test solution, the test solution can be absorbed by each water absorbing portion, and the test can be performed very simply and quickly. Further, the necessary reagents and the like can be contained in the water absorbing portion in advance, so that the test can be performed more quickly.

[Brief description of the drawings]

FIG. 1 is a view showing a test device of the present invention manufactured in Example 2.

[Explanation of symbols]

 1. Water absorption part 2. Diffusion sheet 3. Sample supply hole

 ──────────────────────────────────────────────────の Continued on the front page (72) Inventor Masatoshi Mishina 1075-2 Kitaminami Muro, Yuki City, Ibaraki Pref. Nissui Pharmaceutical Co., Ltd.

Claims (3)

[Claims] 1. A sheet provided with a plurality of mutually isolated water-absorbing portions for reacting a test solution, and a sheet for supplying the test solution to the water-absorbing portions by contacting at least a part of the sheet with the water-absorbing portions. A test device having a water-diffusible sheet, wherein the water-diffusible sheet is a nonwoven fabric and a substrate bonded to each other with a thermoplastic resin. 2. The test device according to claim 1, wherein the thickness of the nonwoven fabric, which is one of the materials of the water diffusion sheet, is 0.05 to 0.6 mm. 3. The test device according to claim 1, wherein the nonwoven fabric of the water-diffusing sheet retains a water absorption of 0.005 to 0.05 ml / cm ² even after lamination.