JPH11246422A - Environmental improving agent in alimentary canal - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain the subject improving agent useful for prevention of constipation or colic cancer by including a platy crushed material obtained by wet crushing a tank bran of grains as an active component. SOLUTION: This improving agent contains a platy wet crushed material of finely crushed tank bran of grains, preferably wheat bran as an active component. Preferably, an amount of the platy crushed material having <=60 μm particle diameter is >=80%. An administrating dose in using the improving agent as a medicine is, in a case of oral administration, about daily 5-20 g for an adult is preferable. Preferably, the tank bran of grains is subjected to a wet crushing treatment after removing contaminant such as starch, protein, lipid and inorganic materials and increasing dietary fibers content before the treatment.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

TECHNICAL FIELD The present invention relates to a gastrointestinal tract environment improving agent used for preventing constipation and colorectal cancer.

[0002]

2. Description of the Related Art Dietary fiber is generally classified into "water-soluble fiber" which is soluble in water and "insoluble fiber" which is insoluble in water, and is known to exert different health effects according to physicochemical characteristics. For example, water-soluble fibers exhibit physiological effects such as cholesterol and carbohydrate absorption inhibitory effects, and are said to be effective in preventing heart disease and diabetes.

[0003] On the other hand, it is known that insoluble fiber has an effect of preventing constipation due to actions such as an increase in the amount of feces and a shortening of a gastrointestinal transit time (also referred to as a gastrointestinal contents retention time). It also has the effect of reducing the concentration of carcinogens such as mutagenic substances in the diet and secondary bile acids produced in the intestine, and promptly excreting and excreting it. (Dietary Fiber, Satoshi Innan, Shuhatsu Kiriyama, 1995)
Year). Examples of the insoluble fiber include bran of cereals such as wheat or barley bran and corn hulls, which contain cellulose, hemicellulose, lignin or the like as a component. Of these, wheat bran has been shown in animal and human administration studies and epidemiological studies to show the best colon fiber cancer-preventing effect of dietary fiber, in addition to its known laxative effect from ancient times. . In order to obtain such an effect, it is necessary to take a large amount or continuous intake of wheat bran. Its use is limited to only a small portion of foods, such as cereals.

[0005] In order to expand the use of wheat bran for food, attempts have been made to improve the texture by reducing the particle size using a dry pulverizer. However, since the multilayer structure of the cell wall, which is mainly composed of fibers, is not completely collapsed, graininess and irritation when eaten are not eliminated, and application to foods such as beverages where the texture is sensitive can be sensed. Is considered difficult. Heller et al. Have pointed out that dry and fine-grained wheat bran has a reduced effect on reducing the digestive tract retention time, which is important for constipation and colorectal cancer prevention (Am. J . Clin. Nutr., 33, 17
34-1744, 1980). Japanese Patent Application Laid-Open No. 63-17767 discloses a dietary fiber composed of flaky ground cereal bran, but does not describe the effect of improving the environment in the digestive tract, which is the subject of the present application.

[0006]

DISCLOSURE OF THE INVENTION The present inventors have developed a cereal rice bran that has a high effect of shortening the residence time of the contents of the digestive tract from the beginning, while improving the environment in the gastrointestinal tract while maintaining the effect and having a good texture. It was an object to provide an agent.

[0007]

Means for Solving the Problems The present inventors have conducted research on the above-mentioned problems. As a result, a flaky ground material obtained by pulverizing grain bran, particularly wheat bran, by a wet grinding process,
The present invention was found to have both a good texture and a high effect of shortening the residence time of gastrointestinal contents, and completed the present invention.

[0008] That is, the gastrointestinal tract environment improving agent of the present invention is characterized by containing, as an active ingredient, a flaky ground material obtained by wet-milling cereal grain bran.

In addition, the digestive tract environment improving agent of the present invention is characterized by containing a flaky ground material obtained by wet-milling wheat bran as an active ingredient.

The particle size of the wet-milled flaky ground material used in the gastrointestinal tract environment improving agent of the present invention is 60 μm.
The following is characterized by at least 80% or more.

[0011] The present invention is also a food for improving the environment in the digestive tract, which comprises the above agent for improving the environment in the gastrointestinal tract.

Further, the present invention is a colorectal cancer inhibitor comprising the above gastrointestinal tract environment improving agent as an active ingredient.

[0013] The present invention also provides a food for preventing colorectal cancer, which comprises the above agent for suppressing colorectal cancer.

[0014]

BEST MODE FOR CARRYING OUT THE INVENTION The flaky grinding material in the present invention is obtained by grinding cereal grains bran while performing a compressive shearing action or a shearing action in a wet state. It has a unique multilayer structure that is broken down by shear stress and miniaturized into flakes.

[0015] Further, as the cereal rice bran of the present invention, for example,
Wheat bran, barley bran, rye bran, corn hulls and the like can be used, but it is more preferable to use wheat bran. These cereal casks are
The wet grinding treatment described below may be performed as it is, but preferably, the wet grinding treatment is performed after removing impurities such as starches, proteins, lipids, and inorganic substances to increase the dietary fiber content.
In addition, by carrying out any of enzymatic treatment, chemical treatment, physical treatment, or a combination of these treatments as appropriate, starch can be used to relax the tissue structure of the seed coat and pericarp of cereals rich in dietary fiber, It can be obtained by removing impurities such as proteins, proteins, lipids, and inorganic substances. In this case, the enzymatic treatment is carried out, for example, using a starch degrading enzyme such as α-amylase and glucoamylase, a protease such as protease, and a lipolytic enzyme such as lipase at pH 3 to 9 and a temperature of 30 to 30.
The addition is performed under the condition of 100 ° C. Chemical treatment
For example, it is carried out by adding an aqueous solution of a mineral acid or an organic acid to the bran of the above-mentioned cereal and heating it under the condition of pH 2 to 5 or adding a surfactant for food and heat-treating it under the condition of pH 3 to 8. . In the physical treatment, for example, operations such as crushing and sieving the above-mentioned cereal bran with a pulverizer such as a pin mill or a jet mill, or suspending and stirring vigorously in water are performed.

The grain bran or the dietary fiber obtained by further purifying them are further pulverized if necessary. The particle size varies depending on the type of machine or dietary fiber used for the treatment, but is usually 32 to 20 depending on the quality and treatment efficiency of the obtained treated product.
It is preferable to use one having a mesh size of 0. In other words, if the particle size is larger than 32 mesh, it will be difficult to perform the treatment in the subsequent wet grinding process efficiently, and if the fine particle size exceeds 200 mesh, the gap between the grinders in the wet grinding process will be reduced. This is because slicing is insufficient and flakes are insufficient, so that good-quality dietary fiber cannot be obtained.

Next, the above-mentioned dietary fiber is ground in a wet state while applying a shear stress. Here, the wet state means a state in which the plant fiber is suspended in a liquid such as water. The dietary fiber concentration of this suspension is preferably from 1 to 20% by weight, more preferably from 2 to 10% by weight. The grinding process can be any device that has the ability to grind the dietary fiber in a wet state. Among them, a grinding mill having a twisting action and a compressive shearing action (Baer mill) as an external grinding force, and a grinding external force as Devices classified as colloid mills having an impact shearing action (see Food Engineering Handbook, edited by Shiro Teramoto, p. 277-302, pulverizers) are preferred. As an example of a colloid mill, see JP-A-63-17767.
Is preferred, but the particle size is 60μ.
In order to obtain at least 80% or more of flake-like ground products having a particle size of less than m, a model that can monitor and control the gap of the grinder with high accuracy is desirable.

The dietary fiber thus obtained preferably has a fiber content of at least 40%, more preferably at least 70%. The fiber content here refers to the Prosky method (Official Methods of Anal
ysis, 15th, Ed., Assoc. Off. Chem., Arlington, VA,
Vol., Sec. 985.29, 1105, 1990).

The flaky ground material obtained by subjecting the above-mentioned cereal grains to brass bran or refined brass bran to increase dietary fiber and subjecting it to wet grinding may be directly used as the agent for improving the environment in the digestive tract or the prophylactic agent for colorectal cancer of the present invention. Yes, but additives that are commonly used in foods and oral pharmaceuticals may be added. Examples of additives used herein include sugars, proteins, lipids, vitamins, plant extracts, animal extracts, gelling agents, flavors, coloring agents, and the like.

The dosage of the agent for improving the environment in the gastrointestinal tract and the prophylactic agent for colorectal cancer of the present invention varies depending on the administration method, the age, weight and condition of the patient. It is preferable that the amount is about 5 g to 20 g per day.

The agent for improving the environment in the gastrointestinal tract and the agent for preventing colorectal cancer of the present invention can be used by adding them to food in an arbitrary range, and can be used as the food for improving the environment in the gastrointestinal tract and the food for preventing colon cancer. The gastrointestinal tract environment improving agent and colorectal cancer preventive agent of the present invention can be appropriately contained in a wide range of foods because they have very small particles and are palatable. Is preferably in the range of 0.5 to 5% by weight, and in the case of solid food such as confectionery, it is desirable to add 1 to 40% by weight. Examples of the food include lactic acid bacteria drinks, fermented milk, soy milk, milk, cheese, dairy products such as ice cream, fruit juice drinks, soft drinks such as sports drinks, soups, biscuits, sweets such as cookies, breads and the like. it can.

[0022]

EXAMPLES (Example 1) 4 kg of wheat bran (fiber content 45
%, Manufactured by Nisshin Flour Milling Co., Ltd.) in 36 kg of water, stirred well, and transferred to a 200-mesh sieve. The residue on the sieve was 60 kg.
In water. This liquid was ground using a colloid mill (Selenium Meister manufactured by Masuko Sangyo), and the micronized product was collected as a precipitate by centrifugation. The dietary fiber content of the obtained paste was 61% per solid. When the particle size of this paste was measured with a particle size distribution analyzer (Coulter LS230), as shown in FIG.
92.7%. Also, the water content of this paste was determined,
When added and dispersed in a commercially available fruit juice so that the solid content became 0.5%, and the beverage was drunk, it was a good throat with no rough feeling.

[0023]

FIG.

(Test Example 1) Twenty-five wheat fibers (fiber content of 70% or more, manufactured by Nitto Flour Milling Co., Ltd.) suspended in water so that the fiber content was increased by washing the wheat bran with water were increased to 20%.
kg was prepared, heated, and stirred to swell. This solution was ground using a colloid mill, and the micronized product in which the multilayer structure was broken was recovered by centrifugation and freeze-dried. Wheat bran washing product made from: Wheat bran (W
B), electron micrographs of WB crushed by a dry mill (Jet Mill; Seishin Industry) (DWB), and WB crushed by wet grinding (WWB) as described above are shown in FIG. (A), FIG. 2 (B) and FIG. 2 (C).

[0025]

FIG. 2

It has been observed that WB is an amorphous crushed fragment having a multilayer structure, and that DWB has fine particles as a whole while having a layered structure. Also W
WB did not have a layered structure, and it was observed that the fibers were finely flaked.

(Test Example 2) [Measurement of sedimentation volume in water] 1 g of each of three kinds of samples (WB, DWB, WWB) was dispensed into a 100 ml beaker, about 50 ml of ion-exchanged water was added, and a stirrer was used. And suspended for 20 minutes. This was degassed for 20 minutes, then transferred to a measuring cylinder, and ion-exchanged water was added to bring the volume to 100 ml. After standing at room temperature overnight, the volume of the settling portion was measured. The settling volume of WWB in water is about twice that of WB,
B was about three times (Table 1).

(Test Example 3) [Measurement of Particle Size] 2.5 g of each of the three samples (WB, DWB, WWB) was taken in a 100 ml beaker, 50 ml of ion-exchanged water was added, and the mixture was homogenized at 6,000 rpm for 1 minute. Was used as a sample. The measurement was carried out with a particle size distribution measuring device and shown as an average particle size (Table 1). The average particle size of WB is about 7 times that of DWB and about 1 times that of WWB.
It was twice.

[0029]

[Table 1]

(Test Example 4) [Measurement of Retention Time of Gastrointestinal Contents] 1) Preparation of Marker: Modified Chromium (Solid Phase Marker) and Cobalt-Ethylenediaminetetraacetate (Co-EDTA, Liquid Phase Marker) Uden et al. Chromium was modified into crystalline cellulose (Oriental yeast) using the method described in (1) to prepare chromium-modified cellulose. In addition, Co-EDTA (Tokyo Kasei) was used as a liquid phase marker.

2) Preparation of a food with a marker A mixture of chromium-cellulose and Co-EDTA added to a fiberless feed and mixed well was used as a food with a marker.

Six-week-old male Fischer rats (CLEA Japan) were purchased and grouped into 5 rats. After feeding with powdered feed (manufactured by Oriental Yeast) and pre-breeding, the animals were transferred to metabolic cages and fed with fiberless feed. Thereafter, the diet was prepared according to the AIN76 composition so that the dietary fiber content in the experimental diet was 10%, and the diet was freely taken. Table 2 shows the measurement results of the rat body weight before administration of the experimental feed and at the end of the experiment (immediately before dissection). No significant difference was observed between the groups in the initial body weight and the final body weight during the experimental period, and similar growth results were shown.

[0033]

[Table 2]

On the evening of the thirteenth day of the administration of the experimental feed, the feeding was interrupted and the rats were fasted.
The experimental feed was administered again. Feces were collected every hour for 54 hours using an automatic feces collection device (manufactured by Shibata Kiki). Table 3 shows the amount of weight gain and feed intake during the measurement of the retention time of the contents of the digestive tract. There was no significant difference in body weight gain and feed intake during the measurement of gastrointestinal contents retention time in each group.

[0035]

[Table 3]

The feces collected over time were placed in a plastic bag with a zipper (Unipack A-4), frozen in a refrigerator overnight, and lyophilized for 48 hours, and then used for measurement of markers in the contents of the digestive tract. Quantification of chromium and cobalt in feces was measured using emission spectroscopy. Mean residence time (MRT: mean reten
tion time) was used. The MRTs of the solid and liquid phase markers were calculated according to the method of analyzing the moment, and the results are shown in Table 4.

[0037]

[Table 4]

As a result of calculating the average residence time (MRT),
The solid phase and aqueous phase MRTs of the DWB group tended to be longer than those of the WB group, respectively, but the solid phase and liquid phase MRTs of the WWB group showed values equivalent to those of the WB group. Also WWB
The MRT of the group was significantly (p <0.05) significantly higher than that of the DWB group.
RT was shortened.

From these results, it is known that the dry-refined wheat bran has a longer MRT as conventionally known, but the wet-refined wheat bran as in the present invention has a smaller particle size even if it has a smaller particle size. It was found that the effect of shortening the MRT did not decrease.

(Test Example 5) [Measurement of Water Content of Cecal Content at Rat Dissection] The water content of cecal content was measured at the time of rat dissection.
Table 5 shows the measurement results.

[0041]

[Table 5]

The water content of the cecal contents of the WB group was determined by DWB
Significantly (p <0.05) higher than the group and significantly (p <0.05) higher than the WWB group.
<0.05) showed a low value. The water content of the cecal contents of the WWB group was significantly (p <0.05) higher than that of the DWB group. Considering this result and the fact that the sedimentation volume of WWB in water is higher than that of WB or DWB (Table 1), WWW MRT
The excellent shortening effect is presumed to be due to the high water retention capacity.

(Test Example 6) [Colon Carcinogenesis Inhibition Test] After preliminarily rearing 75 4-week-old CF1 female mice (Charles River) for 1 week, administration of the experimental feed was started.
The experimental feed was prepared by adding 20% (W / W) of cellulose feed (CL), wheat bran (WB), and wet ground WB (WWB) to base feed (partially modified AIN76A) at 20% (W / W). Feed, WB feed, and WWB feed were used, and the feed and drinking water were all freely available. In addition, from the week when the experimental feed administration was started, Azoxymethane was
(AOM, Sigma Chemical Co.) was administered subcutaneously at a dose of 10 mg / kg body weight once a week for a total of 6 times, 20 animals per group. As a non-administration group, physiological saline was administered to 5 animals in each group.
At week 33, the mice in all groups were dissected, and colon tumors observed under a stereoscopic microscope were pathologically diagnosed, and the number of tumors was shown in Table 6.

[0044]

[Table 6]

The incidence of tumor in the group administered with the chemical carcinogen was not different between the CL feed group, the WB feed group, and the WWB feed group, but the number of tumors per tumor-bearing mouse was as follows: A decrease in the number of tumors was observed in the WWB feed group as compared to the feed group. No tumor was observed in the group not administered with the chemical carcinogen.

[0046]

EFFECTS OF THE INVENTION The flaky ground material obtained by pulverizing cereal grain bran by wet grinding according to the present invention is superior in shortening the residence time in the digestive tract and colorectal cancer compared to fine grain ground by ordinary dry grinding. Since it has an inhibitory effect, the agent for improving the environment in the gastrointestinal tract and the agent for suppressing colorectal cancer of the present invention containing it as an active ingredient are extremely effective for preventing constipation and colorectal cancer. The gastrointestinal tract environment improving agent and colorectal cancer suppressant of the present invention have a very small particle size and are soft and flaky, so that they are much more palatable than conventional dry pulverized products, and can be used in a wide range of foods. It is. The effect is particularly remarkable when blended in a fluid food such as a beverage, in which texture such as mouthfeel and throat is important. The raw materials for the gastrointestinal tract environment improver and colorectal cancer suppressant use wheat bran, which is commercially available as food or food material, and do not pose any safety problems. And continuous intake is possible.

[Brief description of the drawings]

FIG. 1 is a diagram showing the cumulative frequency distribution of wheat bran subjected to wet grinding.

FIG. 2 is a view showing a state of fiber particles photographed using a scanning electron microscope.

────────────────────────────────────────────────── ─── Continued on the front page (51) Int.Cl. ⁶ Identification symbol FI A61K 31/00 635 A23L 2/00 F (72) Inventor Satoka Moriyama 1-1-19 Higashi-Shimbashi, Minato-ku, Tokyo Stock Association Yakult Honsha (72) Inventor Mikiko Sato 1-1-19 Higashi-Shimbashi, Minato-ku, Tokyo Stock Company Yakult Honsha (72) Inventor Yukiko Sakaiya 1-1-1-1 Higashi-Shimbashi, Minato-ku, Tokyo Stock Company Yakult Honsha (72) Inventor Nanami Watanabe 1-1-19 Higashi-Shimbashi, Minato-ku, Tokyo Stock Company Yakult Honsha (72) Inventor ▲ Yoko Takagi 1-1-1, Higashi-Shimbashi, Minato-ku, Tokyo No. 19 Yakult Honsha (72) Inventor Keisuke Matsumoto 1-1-19 Higashi-Shimbashi, Minato-ku, Tokyo (72) Inventor Masami Morotomi 1-1, Higashi-Shimbashi, Minato-ku, Tokyo No. 19 stock company Yakult in the Head Office (72) inventor Kazumi Uchida Tokyo, Minato-ku, Higashi-Shimbashi 1-chome No. 1, No. 19 stock company Yakult in the head office

Claims (6)

[Claims] 1. A digestive tract environment improving agent comprising as an active ingredient a flaky ground material obtained by wet-milling cereal grains bran. 2. The agent for improving the environment in the digestive tract according to claim 1, wherein the rice bran of the cereals is even wheat bran. 3. A flaky ground material obtained by wet-grinding wheat bran and having a particle size of 60 μm or less is at least 80%.
% Or more. 4. A gastrointestinal tract environment improving food containing the gastrointestinal tract environment improving agent according to claim 1. 5. A prophylactic agent for colorectal cancer, comprising the gastrointestinal tract environment improving agent according to claim 1 as an active ingredient. 6. A colorectal cancer preventive food comprising the colorectal cancer preventive agent according to claim 5.