JPH11199436A - Discrimination of cosmetic for sensitive skin - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide cosmetic that can be applied to persons having sensitive skin to chemicals or the like without adverse effect on its stability and intrinsic performance. SOLUTION: The receiver side of the Flantz type diffusion cell equipped with a semi-permeable membrane is filled with the physiological salt solution and a cosmetic is applied to the upper part of the semi-permeable membrane to measure the migration of the paraben in the cosmetic into the physiological salt solution whereby the cosmetic is judged on its sensitivity to the skin based on the inhibition of the migration into the physiological salt solution.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

[0001] The present invention relates to a cosmetic for sensitive skin and a method for identifying the same.

[0002]

2. Description of the Related Art The rapid increase in allergic diseases in recent years has been reported in many media outlets, mainly due to changes in dietary habits and the rapid increase in opportunities for exposure to chemicals. It is said that there is. Against the background of this situation, the number of people with sensitive skin who are susceptible to various stimuli, including atopic dermatitis, is rapidly increasing, and this countermeasure has become a major issue. For people with such sensitive skin, cosmetics with extremely reduced chemical substances that are likely to become irritants have been developed.However, the reduction in chemical substances and their reduction in stability and original functions cannot be avoided. In fact, for example, it has been necessary to carry out packaging or refrigerated storage, which are subdivided according to the amount of use. In addition, there was no way to know whether a cosmetic was suitable for a person with sensitive skin, except by conducting a patch test or the like for each individual. That is, a cosmetic that can be applied to people with sensitive skin without impairing the stability and the original function has been desired, and means for discriminating whether the cosmetic is suitable for people with sensitive skin has been desired.

[0003] Parabens such as methylparaben, ethylparaben, propylparaben and butylparaben are very safe and have excellent preservative action.
It is known that a person with rarely sensitive skin may cause a hypersensitivity reaction, and a means for suppressing such a reaction is desired.

On the other hand, methylcellulose, xanthan gum, bentonite, carboxymethylcellulose, polyethylene glycol having an average molecular weight of 4,000 to 10,000, collagen, cationic ethylcellulose, hydroxypropylmethylcellulose, acrylic acid / alkyl methacrylate copolymer and the like have a thickening action and a moisturizing action. Has been used for a long time in skin external preparations such as cosmetics as a substance having such a substance. However, these substances suppress the percutaneous absorption of parabens, and the cosmetics containing these are parabens-containing cosmetics. However, it has not been known at all that it is a cosmetic that can be used safely even by people with sensitive skin. Whether the cosmetic is suitable for people with sensitive skin is filled with saline on the receiver side of a Franz diffusion cell equipped with a semi-permeable membrane, and the cosmetic is applied on the upper part of the semi-permeable membrane. It was not known at all that what could be predicted by measuring the transfer amount of parabens into physiological saline and using the small transfer amount as an index was measured.

[0005]

DISCLOSURE OF THE INVENTION The present invention has been made under such circumstances, and an object of the present invention is to provide a cosmetic that can be applied to people with sensitive skin without impairing the stability or the original function. .

[0006]

In view of such circumstances, the inventors of the present invention have conducted intensive studies on means for discriminating whether or not cosmetics are suitable for people with sensitive skin, and as a result, they have installed a semi-permeable membrane. Fill the receiver side of the Franz diffusion cell with saline,
A cosmetic is applied to the upper part of the semi-permeable membrane, and the amount of paraben in the cosmetic transferred to physiological saline is measured. Have found what can be foreseen. Further examination based on this result showed that methylcellulose, xanthan gum, bentonite, carboxymethylcellulose, average molecular weight 4
2,000 to 10,000 polyethylene glycol, collagen, cationic ethylcellulose, hydroxypropylmethylcellulose, acrylic acid / methacrylic acid long-chain alkyl copolymer, one or two or more selected from parabens The present inventors have found that a cosmetic that can suppress percutaneous absorption and can be used even by people with sensitive skin can be provided, and has completed the invention. Hereinafter, the present invention will be described in detail.

[0007]

DESCRIPTION OF THE PREFERRED EMBODIMENTS (1) Method for Differentiating Cosmetic for Sensitive Skin of the Present Invention The method for identifying cosmetic for sensitive skin according to the present invention is directed to a Franz-type diffusion cell equipped with a semi-permeable membrane on the receiver side. Filling with physiological saline, applying cosmetic on top of the semi-permeable membrane, measuring the amount of paraben transferred to saline in cosmetic, and using the small amount of this transfer as an index I do. The semi-permeable membrane that can be used in the identification method of the present invention is not particularly limited as long as it is a semi-permeable membrane that does not allow passage of large molecules such as proteins and allows passage of small compounds such as salts. The semipermeable membrane can be used, and examples of such a semipermeable membrane include cellulosic cellulosic paper, a dialysis membrane, a reverse osmosis membrane, Pichit, and the like. Among them, the use of a dialysis membrane is particularly preferable. Among the dialysis membranes, those made of cellulose and having a molecular weight cut off of 12,000 to 14,000 are particularly preferred. This is because the experimental results are in good agreement with the transdermal absorption of parabens. Physiological saline to be filled on the receiver side can contain sugar, buffer salt, etc.
The effect can be sufficiently exerted only with physiological saline. The measurement of the transfer amount is preferably such a length that a sufficient difference in absorbability can be sensed, and specifically, about 16 hours.
If the amount of paraben transferred is less than 50% of the amount applied in about 16 hours, it can be identified as a cosmetic suitable for people with sensitive skin, especially those with sensitive skin who are sensitive to parabens. In the measurement of the transfer amount, the temperature is preferably controlled to a temperature near the skin temperature, that is, 30 to 40 ° C. The transfer amount can be obtained by measuring the amount of paraben in physiological saline on the receiver side by high performance liquid chromatography or the like. Also, simply, it can be measured by measuring ultraviolet absorbance.

(2) Cosmetic of the present invention The cosmetic of the present invention is characterized in that the amount of parabens passing through the semi-permeable membrane is 5 times the amount of the applicate in the above-mentioned identification method.
It is characterized in that it is 0% or less and is applied to people with sensitive skin, especially to people with sensitive skin to parabens. Since the cosmetic of the present invention suppresses the absorption of parabens, it can be used safely even in people with such sensitive skin. In order to obtain such cosmetics, basically, the cosmetics that have been prototyped are evaluated and differentiated according to the above-mentioned identification method, and if the amount passed is greater than 50%, the method is optimized while improving the prescription and the like. In principle, prescription is performed, but such a work can be easily performed by adding a substance that suppresses percutaneous absorption of parabens as described below. Since all of these substances are known as raw materials for cosmetics, there is no problem in using them as paraben absorption inhibitors. The dosage form of the cosmetic of the present invention is not limited as long as it is a cosmetic, for example, a lotion, an emulsion, a cream, a basic cosmetic such as a pack,
Foundation, under makeup, eye color,
Makeup cosmetics such as teak color and lip color,
Preferred examples include hair cosmetics such as hair creams and hair lotions. The cosmetic of the present invention can be formulated using raw materials usually used for cosmetics. Examples of such raw materials include hydrocarbons such as petrolatum and microcrystalline wax, esters such as jojoba oil and gay wax, triglycerides such as tallow, olive oil, higher alcohols such as cetanol and oleyl alcohol, and stearin. Acids, fatty acids such as oleic acid, polyhydric alcohols such as glycerin and 1,3-butanediol, nonionic surfactants, anionic surfactants, cationic surfactants, amphoteric surfactants, ethanol, carbopol, etc. Preferred examples include thickeners, preservatives, ultraviolet absorbers, antioxidants, pigments, powders, and the like.

(3) Paraben Absorption Inhibitor of the Present Invention The paraben absorption inhibitor of the present invention includes methyl cellulose, xanthan gum, bentonite, carboxymethyl cellulose, polyethylene glycol having an average molecular weight of 4,000 to 10,000, collagen, cationic ethyl cellulose, hydroxypropyl methyl cellulose, It is composed of one or more selected from acrylic acid / methacrylic acid long-chain alkyl copolymers. Among them, the average molecular weight is 400
Examples of the polyethylene glycol having an average molecular weight of 0 to 10,000 include polyethylene glycol having an average molecular weight of 6000 and polyethylene glycol having an average molecular weight of 10,000.
Among these, the average molecular weight is 600 from the effect of suppressing percutaneous absorption.
Polyethylene glycol of 0 is particularly preferred. Further, as a long-chain alkyl copolymer of acrylic acid and methacrylic acid, Pemulen TR sold by Goodrich Co., Ltd.
-1 or TR-2 may be used, all of which are raw materials for cosmetics and are used as thickeners and the like.
These substances have the effect of suppressing the transdermal absorption of parabens and preventing irritation to humans with sensitive skin, as shown in the Examples below. The content in which such an effect can be expected is 0.01 to 10% by weight, more preferably 0.1 to 10% by weight.
It is from 0.5 to 7% by weight, more preferably from 0.1 to 5% by weight.

[0010]

The present invention will be described in more detail with reference to the following examples, but it is needless to say that the present invention is not limited to these examples.

<Examples 1 to 6> Lotions (cosmetics) were prepared according to the following recipe. That is, the formulation components were heated and solubilized at 80 ° C. and cooled to obtain a lotion.

1,3-butanediol 7 parts by weight Glycerin 7 parts by weight Polyethylene glycol having an average molecular weight of 1500 4.5 parts by weight Ethanol 5 parts by weight Polyoxyethylene (50) hardened castor oil 5 parts by weight Butylparaben 0.5 parts by weight Water 70 parts by weight Components listed in Table 1 1 part by weight

[0013]

[Table 1]

<Example 7> The suppression of paraben absorption was measured for the cosmetics of Examples 1 to 6 according to the identification method of the present invention, and whether or not the cosmetics were suitable for people with sensitive skin was determined. That is, a dialysis membrane (cellulose tube, hole diameter 24 Å, molecular weight cut off 12000 to 14000) was attached to a Franz diffusion cell filled with physiological saline on the receiver side, and 200 μl of the cosmetic of the above example was applied. After 16 hours, the amount of butyl paraben on the receiver side was measured. In addition, the same study was performed for the control example and the comparative example shown below. Table 3 shows the results. From these results, it can be seen that the cosmetics of Examples 1 to 6 are the cosmetics of the present invention. It is also found that paraben migration can be prevented by the paraben absorption inhibitor of the present invention. Also, it is understood that the average molecular weight of polyethylene glycol is preferably 4000 to 10,000. 1,3-butanediol 7 parts by weight Glycerin 7 parts by weight Polyethylene glycol having an average molecular weight of 1500 4.5 parts by weight Ethanol 5 parts by weight Polyoxyethylene (50) hardened castor oil 5 parts by weight Butyl paraben 0.5 parts by weight Water 70 parts by weight Components listed in Table 2 1 part by weight

[0015]

[Table 2]

[0016]

[Table 3]

<Example 8> The cosmetics of Examples 1 to 6 above,
The effects of the cosmetics of the control example and the cosmetics of Comparative Examples 1 to 5 on paraben-sensitive skin were examined using guinea pigs. That is, a complete adjuvant of Freund's 0.1 m was given to 120 Hartley white guinea pig females.
0.1 mg of butyl paraben and 0.1 ml of physiological saline
Was injected intradermally once a day, four times every other day to increase the sensitivity to parabens. Two weeks after the last intradermal injection, a 0.1% butyl paraben olive oil solution was closed-patched for 24 hours, and after removing the patch, the skin reaction was observed according to the Draise's criteria, and animals with a response of ± or higher were selected. There were 13 animals (responses were ±). The back of the animal which became sensitive to the paraben was shaved 48 hours after the patch was removed, and the cosmetics of Examples 1 to 6, Control Example and Comparative Examples 1 to 5 were put on a lint cloth having a diameter of 2 mm by 0.01.
ml and closed for 24 hours. Two hours after patch removal, skin reactions were observed according to Draise criteria. The results are shown in Table 4 as the number of appearance cases. From this table, it can be seen that the cosmetic of the present invention is very safely applied to animals sensitive to parabens. Furthermore, this proved that the identification method of the present invention was appropriate. (Draise criteria) ++: Reaction with edema +: Reaction with obvious erythema ±: Reaction with indistinct erythema-: No reaction

[0018]

[Table 4]

Example 9 An emulsion was prepared according to the following formulation. That is, the components (a) and (b) were dissolved by heating at 80 ° C., and (b) was gradually added to (a) for emulsification, followed by stirring and cooling to obtain an emulsion. The transfer amount of butyl paraben measured by the method of Example 7 is also shown in Table 5. It can be seen that the percutaneous absorption inhibitor of paraben of the present invention suppresses paraben migration and enhances the safety of the cosmetic of the present invention for people with sensitive skin. I-cetanol 0.2 parts by weight stearic acid monoglyceride 1 part by weight polyoxyethylene (25) stearic acid 1.4 parts by weight butyl paraben 0.5 parts by weight liquid paraffin 10 parts by weight b 1,3-butanediol 5 parts by weight Table 5 0.3 parts by weight of water 81.6 parts by weight of water

[0020]

[Table 5]

[0021]

According to the present invention, it is possible to provide a cosmetic that can be applied to people with sensitive skin without impairing the stability or the original function.

──────────────────────────────────────────────────続 き Continued on the front page (51) Int.Cl. ⁶ Identification code FI A61K 7/00 A61K 7/00 M // B01D 61/24 B01D 61/24 G01N 33/50 G01N 33/50 Q 33/53 33 / 53 Q

Claims (5)

[Claims] 1. A saline solution is filled on the receiver side of a Franz diffusion cell equipped with a semi-permeable membrane, a cosmetic is applied on an upper portion of the semi-permeable membrane, and paraben in the cosmetic is transferred to the saline. A method for identifying cosmetics for sensitive skin, characterized in that the amount of the cosmetic is measured and the small amount of the migration is used as an index. 2. The method for identifying cosmetics for sensitive skin according to claim 1, wherein the semi-permeable membrane is a dialysis membrane. 3. The method for identifying cosmetics for sensitive skin according to claim 1, wherein the sensitive skin is sensitive to paraben. 4. The method for identifying cosmetics for sensitive skin according to claim 1, wherein paraben migration to the receiver side is 50% or less of the total amount of paraben. A cosmetic for sensitive skin. 5. The cosmetic for sensitive skin according to claim 4, which comprises one or more selected from paraben absorption inhibitors shown in the following (a). A) methylcellulose, xanthan gum, bentonite, carboxymethylcellulose, polyethylene glycol having an average molecular weight of 4,000 to 10,000, collagen, cationic ethylcellulose,
Hydroxypropyl methylcellulose, acrylic acid / methacrylic acid long-chain alkyl copolymer