JPH11196667A - Spawn scraper - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a spawn scraper that can scrape up and remove the spawn on the surface and can simply sterilize the mouth of the spawn vessel, immediately before the spawn is fed into the mushroomculturing vessel or into the culturing bags or pouches. SOLUTION: This spawn scraper is equipped with the seed spawn vessel setter 13 to which the seed spawn vessel is set in the inverted state, the aged spawn scraping mechanism 54 that has a spawnscraping blade 65 for scraping up the aged part from the spawn surface in the spawn vessel, the spawn sterilizer 55 that is equipped with a heater for sterilizing the mouth 10A of the spawn vessel 10 into which the spawn scraping blade 65 is inserted and the spawn vessel rotor that rotates the spawn vessels.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a seed bacterium scraper for scraping and removing a surface portion of a seed bacterium contained in a seed bacterium before inoculating a cultivation container or a cultivation packaging bag with a culture medium filled with a mushroom inoculum. It concerns the device.

[0002]

2. Description of the Related Art Conventionally, as a method for artificially cultivating mushrooms using a culture medium containing a sawdust or rice bran as a main component and a nutrient source, a culture medium is filled in a cultivation container, sterilized, and the filled cultivation is performed. After inoculating seeds of mushrooms such as enoki mushroom and maitake in a container, cover the mouth of the cultivation container with a lid, close the container, and cultivate the bacteria under predetermined temperature and humidity conditions for industrial cultivation. Alternatively, the culture medium is filled in a plastic packaging bag, the bag opening of the filled culture medium-filled packaging bag is turned back and closed, and the culture medium is sterilized with the bag in this state. There is a method of inoculating a seed bacterium in a filling and packaging bag, culturing the bacterium under predetermined temperature and humidity conditions, and industrially cultivating the bacterium.

[0003] By the way, as a preparatory work before inoculating a seed culture into a culture medium in a conventional cultivation container or cultivation packaging bag, a surface portion of a hardened and aged seed bacterium in a seed germ container is scraped. At this time, various bacteria adhere to the inside of the inoculum container or the mouth side of the container, and invasion of the inoculum into the cultivation container or cultivation bag during the next step of inoculation of the inoculum by the inoculation device. To prevent this, the sterilization treatment is performed in the pretreatment stage of the inoculum inoculation device.

[0004] In the pre-inoculation step of the inoculum by the inoculum inoculation device, conventionally, the inoculum scraping operation described above is performed by using an alcohol for sterilization treatment and an alcohol lamp.
At present, it is done manually by using a scraper stick that scrapes out the inoculum, at this time, first light an alcohol lamp, take up the inoculum container, and turn the mouth of the container diagonally downward. While holding the tip, the tip of the scraping rod was immersed in alcohol and subjected to flame disinfection on an alcohol lamp.Then, the scraping rod was inserted into the mouth of the inoculum container, and only the hardened surface of the inoculum in the inoculum container was scraped. And remove the inoculum on the surface from the inoculum container.
Further, the mouth of the seed container is also sterilized by spraying with alcohol, wiping with alcohol, or sterilizing by heating.

[0005]

In the meantime, the manual operation of scraping and removing the inoculum and the sterilization process require a lot of time because the operation is performed by hand, and the inoculation of the inoculum by a scraping rod is required. Variations occur in the depth of scraping from the mouth of the container, and in order to determine the depth due to scraping and removal, the mouth of the inoculum container is turned upward to determine the scraping situation. In the inoculation device that fills the cultivation container or the cultivation bag with a predetermined amount of the inoculum, which is the next step, and inoculates the inoculation device that inoculates the inoculum, the inoculum is subjected to the inoculum scraping and removal operation. It had to be set in a state where it was not maintained, which required time and effort. In addition, the sterilization state varies, and there is a problem in management.

[0006] Therefore, the present invention is to automatically perform a scraping operation of a seed bacterium, which is performed as a preparation for inoculation of a seed bacterium to be supplied into a cultivation container or a cultivation bag, and to perform a simple sterilization treatment while scraping and removing the seed bacterium. It is an object of the present invention to provide an inoculum scraping device which can be used.

[0007]

According to the present invention, there is provided a seed container mounting member for disposing a seed container in an inverted state, and a surface portion of an aged seed cell in the seed container mounted on the seed container mounting member is scraped. A seed surface scraping and removing mechanism having a bacteria scraping blade, a sterilizing device for sterilizing an opening of the seed bacteria container into which the bacteria scraping blade is inserted, and rotating the seed microorganism container mounted on the seed microorganism container mounting member. With a seed container rotation mechanism

[0008] Further, the sterilization apparatus comprises heating means.

The heating means comprises a hot air generator.

[0010] The sterilizing apparatus is provided in close proximity to the scraping blade of the seed bacteria surface scraping and removing mechanism and sterilizes the bacterial scraping blade.

[0011]

DESCRIPTION OF THE PREFERRED EMBODIMENTS In the inoculum inoculation apparatus, in the introductory step of the inoculum scraping mechanism of the inoculum inoculation apparatus, the inoculum container mounting member for disposing the inoculum container in an inverted state, Sterilization treatment comprising a seed bacteria surface scraping and removing mechanism having a fungus scraping blade for scraping the surface portion of the aged fungus in the fungus container, and heating means for heating and sterilizing the mouth of the fungus container into which the fungus scraping blade is inserted. Equipment and
By having a seed container rotating mechanism for rotating the seed container mounted on the seed container mounting member, by setting the seed container on the seed container mounting member, the surface of the seed can be easily scraped and removed. While continuously performing heat sterilization of the mouth of the inoculum container around the entire mouth,
In addition, the inoculum container that has scraped the surface of the aged inoculum can be sent to the inoculum inoculation process provided in the inoculum inoculation device, which automatically scrapes the inoculum surface, scrapes off the inoculum, and easily sterilizes it. Processing can be performed.

[0012]

Embodiments of the present invention will be described below with reference to the accompanying drawings. In the present embodiment, an example is described in which the inoculum scraping device is provided at a pre-process position of the inoculum inoculation device.

In the inoculum inoculation apparatus to which the present invention is applied, a transfer conveyor 2 is provided over substantially the entire length of the main body frame 1.
The transfer conveyor 2 is provided so as to be started and stopped by operating a switch on an operation panel 3, and an endless chain 4 is extended in parallel between a plurality of sprockets provided at both ends of the main body frame 1. ing.

Then, a cultivation container 6 filled with a culture medium containing nutrients mainly composed of sawdust or rice bran and sealed with a lid 5 is transferred, for example, a plastic container 7 containing four columns and four rows. The transfer conveyor 2 is placed on the chain 4 at the start end, and when the transfer conveyor 2 is started, the container 4 can be conveyed toward the transfer end by the movement of the chain 4.

At a substantially central position of the main body frame 1, as shown in Japanese Patent Application Laid-Open No. 5-103543 previously proposed by the present applicant, cultivation vessels 6 are arranged and stored in a plurality of rows. The container 7 is transferred from the loading side to the inoculum inoculation position, and at the inoculum inoculation position, the transfer conveyor 2 is arranged in a horizontal row of the cultivation container 6 in the transfer direction of the container 7.
In this case, the transfer conveyor 2 is stopped upon detecting that the cultivation container 6 has reached an appropriate inoculum inoculation position, and the horizontal row in the container 7 is detected. And a lid opening / closing device for opening and closing the lids 5 of the cultivation containers 6 arranged in a horizontal line at the inoculum inoculation position by the container positioning device 8. 9 are provided.

The lid 5 of the cultivation container 6 by the lid opening / closing device 9
When the cultivation container 6 is opened, the inoculation device 11 is located above the lid opening and closing device 9 and scrapes a predetermined amount of the inoculum S in the inoculum container 10 to inoculate the opened cultivation container 6.

The inoculum inoculation device 11 constructed on the upper side of the main body frame 1 is an example of the inoculum inoculation device as disclosed in Japanese Patent Application Laid-Open No. 8-126433 previously proposed by the present applicant. The device 11 has a substantially flat plate-shaped mounting hole 12 into which the mouth portions 10A of the four inoculum containers 10 corresponding to the number of the horizontal cultivation containers 6 accommodated in the container 7 are inserted in an inverted state. Is provided on a rail-shaped guide member 14 attached to both sides of the main body frame 1.

In this embodiment, the guide member 14 can be placed at both ends of the seed container mounting member 13 and can be slid by its own weight at a predetermined angle so as to be slidable by the guide member 14. The guide member 14 is provided at its lowermost end with a stopper 14A for defining a sliding movement area of the seed container mounting member 13.

On both sides of the main body frame 1 corresponding to the position where the seeds S are scraped out of the seed container 10, flat columns 15 are erected along the inclination angle of the guide member 14, respectively. An air cylinder 16 that supports or releasably supports the inoculum container 10 is attached to each of the columns 15 arranged in an angled state,
A support member 17 is bridged and fixed between the upper ends of the pistons of the air cylinders 16, and the support member 17 is disposed in an upper space of the guide member 14 so as to be inclined in a state parallel to the guide member 14. I have.

The support member 17 includes a seed container 10.
A plurality of sprockets 18 serving as rotation transmitting members are rotatably supported via rotation shafts 19 at positions corresponding to the bottom portions 10B of the inoculum container 10 at the lower ends thereof. A disk-shaped pressing plate 20 of approximately the same size is attached and fixed to the rotating shaft 19, and the support member 17, the rotating shaft 19 attached to the supporting member 17, the pressing plate 20, etc. A pressing support member 21 that presses the bottom portion 10B is configured. In this case, the disk-shaped pressing plate 20 that forms a part of the pressing support member 21 is prevented from rotating with respect to the rotation shaft 19 and is resilient. The bottom 10 of the inoculum container 10
B is configured to be urged.

A motor 22 having a gear head is mounted on and fixed to the support member 17 facing the sprocket 18, and a driving sprocket 23 is provided on a rotating shaft of the motor 22. And the sprocket 18 and the support member 1 serving as the rotation transmitting member for rotating each pressing plate 20
7, an endless roller chain 25 is bridged between the driven sprocket 24 and the driven sprocket 24.
And the sprockets 18, 23, 24 and the roller chain 25 constitute a seed container rotation drive member 26. The rotation of the motor 22 causes the rotation of each sprocket 18 via the endless roller chain 25, whereby the rotation of each sprocket 18 is transmitted. The pressure plate 20 side fixed to the sprocket 18 is configured to be rotated via a rotation shaft 19.

At the position where the seed S is scraped out of the seed container 10, the seed container mounting member 1 is attached to both ends of the support member 17 which constitutes a part of the pressing support member 21.
Positioning shafts 27 are provided to project right and left in pairs toward fitting 3, and fitting holes 2 are respectively formed at both ends of the inoculum container mounting member 13 facing the tip of the positioning shaft 27.
The positioning shaft 27 and the fitting hole 28 constitute a position regulating member 29 for fixing and supporting the seed bacterium mounting member 13 on the guide member 14.

In the inoculum inoculation apparatus according to the above-described embodiment, when the inoculum container 10 is loaded and held in the inoculum container mounting member 13, the inoculum container 10 filled with the inoculum S is formed into an inverted plate shape. Seed container mounting member 13-4
The mouth portion 10A of the inoculum container 10 is mounted and set in each of the mounting holes 12, while, as shown in FIG.
6, the piston of the air cylinder 16 is maintained at the raised position, and the rising of the piston causes the support member 17 and the pressing plate 20 attached to the support member 17 to move.
Positioning member 2 attached to side and support member 17
9, the seeding vessel mounting member 13 previously set in a state where the seed vessel 10 was inverted was positioned below the pressing plate 20 which is the position where the seed S was scraped out. The support member 17 fixed to the piston is also lowered by placing the piston on the guide member 14 and then lowering the piston of the air cylinder 16, and attached to the support member 17 as shown in FIG. Press plate 2
0 and the positioning shaft 27 of the position regulating member 29 is lowered, and the respective inoculum containers 10
The bottom portion 10B is pressed and supported, and the tip of the positioning shaft 27 has a flat plate-shaped inoculum container mounting member 13.
Hole 28 which constitutes a part of position regulating member 29 provided in
, Whereby the inoculum container mounting member 13 slidably mounted on the guide member 14 is fixedly supported at a predetermined position by the insertion of the positioning shaft 27 as the position regulating member 29 and the fitting hole 28, The advance preparation in the step of scraping out the inoculum S in the container 10 is completed.

In addition, as described above, the seed-bacteria container mounting member 13 in this embodiment has a number of cultivation containers 6 accommodated in the container 7 in a row unit (four).
Four mounting holes 12 for receiving the mouth portions 10A of the various germ containers 10 are formed, and support shafts 30 are respectively erected between the mounting holes 12, and upper end portions of the respective support shafts 30 are provided. The trunk 10C of the inoculum container 10
Is attached and fixed. The trunk 31 of each of the inoculum containers 10 arranged in an inverted state is supported by the piece 31 so as not to fall down.

The inoculum inoculation device 11 is inserted into the inoculum container 10 through the mounting holes 12 of the inoculum container mounting member 13.
There is provided an inoculum scraping mechanism 32 for scraping out the inoculum S inside the inoculum. The inoculum scraping mechanism 32 is formed by a scraping shaft 34 having a scraping blade 33 at the tip thereof. The rotation transmission of the motor 35 serving as a member is transmitted via a chain 36, and the scraping shaft 34 is mounted on the plate 37 of the main body frame 1 together with the motor 35.

Further, the scraping shaft 34 provided with the scraping blade 33 is disclosed in Japanese Patent Laid-Open No. 5-103 previously proposed by the present applicant.
No. 543, etc., the scraping blade 33
The plate 37 which supports each scraping shaft 34 is provided so as to be able to move up and down with the rotation of the screw shaft. In this embodiment, a screw shaft 39 is screwed into a female screw cylinder 38 provided on the plate 37, and this screw shaft is Reference numeral 39 is connected to a motor 41 serving as a scraping-up driving member via an endless chain 40. The rotation of the motor 41 is transmitted to the screw shaft 39 via the chain 40, and the rotation of the screw shaft 39 is controlled. With the rotation, the plate 37 is raised, and this plate 3
The scraping shaft 34 provided with the scraping blade 33 mounted on the guide 7 is guided upward.

When the scraping shaft 34 reaches the rising end, the motor 41 serving as the scraping and rising driving member rotates in the reverse direction so as to return to the falling end, and the reverse rotation causes the plate 37 to move through the screw shaft 39. Is configured to return downward.

Further, the 4 provided on the inoculum container mounting member 13
Inoculation hoppers 42 for guiding and storing the inoculum S are provided below the mounting holes 12, respectively, and a shutter 43 for opening and closing the opening lower end is provided at the lower end of each inoculum hopper 42. Have been.

Further, below the shutter 43, there is provided a seed supply tube 44 for guiding the seed S to the mouth of the cultivation container 6 when the shutter 43 is opened. Along with the opening / closing device 9, it is movably provided via a link arm, and is interlocked with the closing operation of the lid 5 by the lid opening / closing device 9 according to the operation of the cylinder 45, and the inoculum supply cylinder 44.
Is movably disposed so as to retreat to a position away from the cultivation container 6.

In the inoculum inoculation apparatus to which the present invention is applied, the inoculum container mounting member 1 is located at an upper position of the main body frame 1, that is, on the side where the guide member 14 is carried in.
A seed cell supply mechanism 46 is provided, which allows a predetermined number of cells 3 to be placed and the seed cell container mounting member 13 to be supplied intermittently sequentially.

In the inoculum container supply mechanism 46, an endless chain 48 is laid in parallel between respective sprockets 47 provided at both ends of the main frame 1 at a flat upper surface portion thereof. A motor 49 serving as a carry-in / supply driving unit is connected to one portion of the motor 47 via a gear, and the rotation of the motor 49 causes the seed container mounting member 13 placed on the endless chain 48 to be intermittently fed. It is configured as follows.

On both sides of the endless chain 48 bridged between the sprockets 47, guide side plates 50 for guiding the seed container mounting member 13 are provided.
The guide member 14 is disposed adjacent to the unloading side of the endless chain 48 at a predetermined angle.
A delivery guide piece 51 is attached and fixed to the guide side plate 50 located at the unloading side end of the delivery guide piece 51.
When the inoculum container mounting member 13 shifts from the chain 48 to the guide member 14 arranged obliquely,
The rear end side of the inoculum container mounting member 13 is prevented from rising up from the guide member 14 and inverting.

Also, on the carry-out side of the guide member 14,
A seed container discharging mechanism 52 to which the seed container mounting member 13 is transferred is provided, and the seed container discharging mechanism 52 is provided.
Is formed in a frame shape as a whole, and the rail-shaped guide member 1
The carrying-out rails 52A are arranged on both sides in accordance with the width of the rails 4, and a stopper for preventing falling is formed at the carrying-out end of the carrying-out rail 52A.

The above-mentioned stopper portion 14A is provided with a cylinder 5 as a stopper releasing means so as to be able to protrude and retract with respect to the conveying surface of the rail-shaped guide member 14.
When the stopper 14A is retracted by the operation of the cylinder 53, the inoculum container mounting member 13 loaded with the inoculum container 10 in which the inoculum S on the rail-shaped guide member 14 is empty is inclined. The rail 5 for unloading, which is a sliding transfer according to
It is formed so that it is delivered to the 2A side, and the seed container mounting member 13 is positioned and stopped by the stopper for preventing fall.

In the present invention, as shown in FIGS. 6 to 9 in addition to FIG. 2 described above, between the inoculum inoculation device 11 of the inoculum inoculation device and the inoculum container supply mechanism 46, ie, the inoculum inoculation device A seed surface scraping and removing mechanism 54 that scrapes a surface portion of the aged seed S in the seed container 10 placed in an inverted state in a process preceding the step of scraping the seed S by the seed seed scraping mechanism 32 of the eleven seeds; Some of the components of the surface scraping and removing mechanism 54 and the inoculum container 10
And a sterilization treatment device 55 comprising a heating means for heating and sterilizing the mouth 10A of the inoculum is provided on the main body frame 1 of the inoculum inoculation device.

In this embodiment, the seed container mounting member 13 fed from the seed container supply mechanism 46 is stocked in the middle of the rail-shaped guide member 14, and the seed cell scraping and removing mechanism 54 and the sterilizing apparatus 55 are stored. The seed inoculation pretreatment transport mechanism 56 for feeding the seed container 10 together with the seed container mounting member 13 is configured.

The seed inoculation pretreatment transport mechanism 56 is formed in a frame shape with a stopper for a pretreatment step for stopping the seed container mounting member 13 sent from the seed container supply mechanism 46 at a predetermined position. Mounting slide member 5
The frame-shaped mounting slide member 57 is provided so as to be able to reciprocate together with the slider portion 60 via a guide rail 59 attached to the fixed frame 58 side.

In this embodiment, the placing slide member 5 described above is used.
As the reciprocating mechanism on the 7 side, the driving source is constituted by a motor 61, and the rotational driving of the motor 61 is performed via a pinion and a rack (not shown).
Is configured to reciprocate, but may be configured by driving means such as a cylinder.

In the reciprocating movement area of the placing slide member 57, the seed surface scraping and removing mechanism 54 and the sterilizing apparatus 55 are provided.

As a pretreatment step as a pre-step of inoculating the culture medium in the cultivation container 6 or a culture bag in a cultivation packaging bag (not shown), a surface portion of the hardened and aged inoculum S in the inoculum container 10 is scraped. The inoculum surface scraping and removing mechanism 54 for removing the inoculum is provided on the fixed panel 62 attached to the main body frame 1 of the inoculum inoculation device 11 by the female screw bearing 6.
3 is screwed into the female screw bearing portion 63 with a germ scraping screw shaft 64, and the distal end of the germ scraping screw shaft 64 is inserted into the seed bacterium container 10 through each mounting hole 12 of the seed germ container mounting member 13. A fungus scraping blade 65 for scraping out only the surface portion of the aged inoculum S is provided.
A motor 66 is connected to the lower end of the motor 4 for guiding the ascending blade 65 while rotating the same.

The motor 66 is fixed to a motor mounting plate 67, and a slider 68 is mounted on the motor mounting plate 67. The slider 68 is mounted on a guide rail 69 mounted on the fixed panel 62. It is mounted so that it can move up and down along.

Accordingly, the rotation of the motor 66 drives the germ scraping screw shaft 64 to rotate, so that the germ scraping screw shaft 64, which is supported by the female screw bearing portion 63, rises as it rotates, and the fixed panel 62 rotates. The motor 66 attached to the fixed panel 62 is also guided upward through the slider 68 along the guide rail 69 attached to the motor.

The germ scraping screw shaft 64 provided with the germ scraping blade 65 on the upper end side is moved when the germ scraping blade 65 reaches a predetermined depth from the mouth portion 10A of the inoculum container 10, that is, the inside of the inoculum container 10 The motor 66 reversely rotates so as to return to the descending end when it reaches the rising end which has a depth dimension that scrapes off only the surface portion of the hardened and aged inoculum S, and by this reverse rotation, the motor 66 and the screw shaft 64 are scraped out. The germ scraping blade 65 and the motor 66 are configured to return downward.

Further, in this embodiment, a sterilization processing apparatus 55 comprising a heating means is constructed in order to sterilize the vicinity of the mouth 10A of the seed container 10.

A sterilization apparatus 55 comprising this heating means
Is composed of a hot air generating device. The hot air generating device has a heater element 72 housed inside a transparent quartz tube 71 and a connecting tube 7 provided at the rear end side of the quartz tube 71.
3 is attached to the body frame 1 side of the inoculum inoculation device, and a flexible hose 74 is connected to the rear end of the connecting tube 73. The supply port side of the hose 74 is set at a predetermined pressure by an air supply source (not shown). Hot air blown from the tip of the quartz tube 71 containing the heater element 72 is supplied by the supply of air from the air supply source while the heater element 72 is energized. It is blown out toward the mouth 10A of the container 10 to heat it.

A seed container rotating mechanism 75 for rotating the seed container 10 by pressing the bottom 10B of the seed container 10 held in an inverted state is provided above the seed container 10 mounted on the seed container mounting member 13. ing. This inoculum container rotation mechanism 76 attaches a motor 76 to the fixed frame 58,
A disk 78 whose surface is formed of a member such as rubber is attached to the end of a rotating shaft 77 of the motor 76. The disk 78 rotated by the motor 76 is attached to the seed container 1.
By pressing against the bottom 10B of the zero, the seed container 10 is rotated by frictional rotation of the disk 78.

Thus, the seed container 10 is rotated by the seed container rotating mechanism 75 with respect to the sterilization processing device 55 including the hot air generator fixedly disposed toward the mouth 10A of the seed cell container 10. Seed container 1
The entire periphery of the mouth 10A of the inoculum 10 is exposed to the hot air from the hot air generator, and the entire periphery of the mouth 10A of the inoculum container 10 can be heated and sterilized.

The motor 76 of this seed container rotating mechanism 75 is attached to an elastic portion 79 provided as a part of the fixed frame 58, so that the seed seed surface scraping and removing mechanism 52 is moved by the seed inoculation pretreatment transport mechanism 56. The disc 78 is elastically in contact with the bottom portion 10B of the seed container 10 carried into the container, and the rotational driving force of the motor 76 can be transmitted to the seed container 10.

The disc 78 presses and holds the bottom portion 10B of the seed container 10 toward the seed container mounting member 13 to scrape the surface of the seed microorganism S into the seed container 10 to scrape the surface of the seed microorganism S. This prevents the inoculum container 10 from rising from the inoculum container mounting member 13 with respect to the pushing force of the blade 65.

In this embodiment, the sterilizing apparatus 55 is used.
Is provided by disposing the tip of the quartz tube 71 of the hot air generator, which is a heating means, close to the position of the bacteria scraping blade 65 of the seed bacteria surface scraping and removing mechanism 54, thereby heating and sterilizing the mouth 10A of the seed microorganism container 10 described above. In addition, heat sterilization of the bacteria scraping blade 65 can be performed.

Next, the operation of the inoculation step using the conventional inoculum inoculation device in the above-described configuration will be briefly described. In particular, the inoculation pretreatment step of the inoculum S by the inoculum inoculation device 11 provided in the inoculum inoculation device, that is, The inoculum surface scraping and removing mechanism 54 for scraping the surface of the aged inoculum S in the inoculated inoculum container 10 and the sterilization apparatus 55 will be mainly described.

First, a container 7 containing a predetermined number of cultivation containers 6 with lids 5 is placed on the transfer conveyor 2 of the inoculum inoculation device, and the container 7 is transported. Is reached, the transfer conveyor 2 stops, and then the cultivation container 6 is positioned and fixed at the seed inoculation position with the container positioning device 8 regulating the vertical direction and the center position of the cultivation container 6.

At the time of positioning by the container positioning device 8, in this embodiment, the motor 35, which is a scraping rotary drive member, is also driven for a preset time to drive the scraping shaft 3.
By rotating the seed 4, the seed S in the seed container 10 is scraped out.
The scraped seed bacteria S are stored in the lower end of the seed hopper 42.

Next, after the positioning by the container positioning device 8 is performed, the lid 5 of the cultivation container 6 is rotated to the lid opening position by the lid opening / closing device 9 with the operation of the cylinder 45, and interlocked with this lid opening operation. The inoculum supply cylinder 44 moves right above the cultivation container 6 from which the lid 5 is removed, and the inoculum supply cylinder 44 is positioned on the axis connecting the inoculum hopper 42 and the cultivation container 6.
Is arranged.

When the lid of the lid opening / closing device 9 is completely opened, the shutter 43 is opened so that the seed hopper 42 is opened.
The inoculum S inside the inoculum is supplied to the cultivation container 6 through the inoculum supply cylinder 44 in a horizontal row, and the shutter 43 closes the lower open end of the inoculum hopper 42 after the inoculation of the inoculum S is completed. At the same time, the inoculum supply cylinder 44 is retracted and returned from the position of the cultivation container 6 by the return operation of the cylinder 45, and at the same time, the lid opening and closing device 9 is rotated back to inoculate the inoculation with the inoculum S. Is fitted.

In this embodiment, when the inoculum S in the inoculum container 10 becomes empty, the support member 17 is raised by the operation of the air cylinder 16, and the bottom portion 10B of the inoculum container 10 is moved by the ascending operation. Is released at the same time as the pressing support member 21 for pressing is released, the positioning shaft 27 which constitutes a part of the position regulating member 29 is also raised, so that the pressing and fixing support of the inoculum container 10 is released and the positioning shaft 27 is released. Comes out from the fitting hole 28 provided in the seed container mounting member 13, and as a result, the seed container mounting member 13, which has been loaded with the vacant four seed cells 10, moves into the position regulating member 29. From the guide member 14, which slides along the slope of the inclined rail-shaped guide member 14, and the guide member 1
The position of the inoculum container mounting member 13 loaded with the four inoculum containers 10 which is restricted in position by the stopper portion 14A provided on the lower end portion of the slope 4 at the same time as the next, is set along the slope of the guide member 14 at the same time. The inoculum S slides to the position where the inoculum S is scraped out, and the four inoculum containers 10 filled with the inoculum S in the standby state and the four inoculated inoculum containers 1
Switching to 0 is automatically performed.

After the replacement operation of the inoculum container 10,
When the piston of the cylinder 16 descends, the support member 17 fixed to the piston descends. With this descending operation, the positioning shaft 27 attached to the support member 17 and the pressing plate 20 descend, and the positioning is performed. The tip of the shaft 27 is inserted into the fitting hole 28 provided in the seed container mounting member 13, and the bottom portions 10 </ b> B of the four seed cells 10 newly supplied by the pressing plate 20 are pressed and supported. , Various bacteria containers 1
Preparation for scraping inoculum S within 0 is performed, and then, as described above, the inoculation operation of inoculum S is sequentially performed by the rotational drive of the scraping blade 33 and the inoculum container 10, and the inoculum S is filled into the cultivation container 6. Thus, the inoculation work is performed sequentially.

Accordingly, the cultivation container 6 in the container 7 is sequentially operated in a horizontal row unit by sequentially operating the series of processes.
Can be continuously inoculated with inoculum S.

In the above-described embodiment, the operation of the seed bacterium surface scraping and removing mechanism 54 and the sterilizing apparatus 55 provided in the inoculum inoculation apparatus, which is the main object of the present invention, will be described.

In the above-described inoculum inoculation apparatus, when the inoculum container 10 is supplied or when the inoculation and switching operation of the inoculum container 10 is performed, the inoculum container supply mechanism 46 provided on the carry-in side of the guide member 14 carries in the inoculation supply drive means. The seed container mounting member 13 placed on the endless chain 48 is intermittently fed by the rotation drive of the motor 49, and the seed container mounting member 13 placed on the carry-out side end of the chain 48 by this feed. Is newly fed into the supply side of the guide member 14 arranged at a predetermined angle.

At this time, in the middle of the guide member 14,
As shown in FIG. 2 or FIG. 7, a seed container pretreatment transport mechanism 56 is provided, and a mounting member formed in a frame shape and provided with a stopper for a pretreatment step constituting the seed container pretreatment transport mechanism 56. The inoculum container mounting member 13 is fed onto the placing slide member 57 and stocked at a predetermined position.

Next, with the rotation of the motor 61, as shown in FIG. 11, from the position shown in FIG. Is moved forward to the final end position together with the slider portion 60 via the guide rail 59 attached to the fixed frame 58 side, and is sent out. The bacterial scraping blade 65 of the seed surface scraping and removing mechanism 54 disposed at the final end position thereof in correspondence with the stop position of the seed cell container 10 on the front side is moved to the motor 66.
, And is guided upward through the rotation of the inoculum container 10, fed into the interior of the inoculum container 10 from the mouth 10 A of the inoculum container 10, and driven up by a predetermined amount of depth dimension. I try to scrape off the part.

At this time, the inoculum container 1 placed in an inverted state
0 is the disk 7 of the inoculum container rotating mechanism 75.
8, the inoculum container 10 is rotated by the rotation of the motor 76, and the sterilizing apparatus 5 comprising a hot air generator is provided.
5, by rotating the inoculum container 10 at least one turn with respect to the hot air blowing position of the fixedly disposed hot air treatment device, the entire periphery of the mouth 10A of the inoculum container 10 is a heating means. Therefore, the entire periphery of the mouth 10A of the inoculum container 10 is heated and sterilized.

In this way, even if the inoculum S, whose surface has been scraped off and removed by the inoculum surface scraping and removing mechanism 54, touches the mouth 10A of the inoculum container 10 and adheres various bacteria, The entire periphery of the mouth 10A of the inoculum container 10 is heated and sterilized by the sterilization treatment device 55, which is a heating means composed of a hot air generator, so that inoculation of inoculum S with various bacteria during inoculation of inoculum performed in a later step is prevented. What you get.

Subsequently, when the bacteria scraping blade 65 reaches a predetermined depth dimension from the mouth portion 10A of the inoculum container 10, that is, the depth at which only the surface portion of the hardened and aged inoculum S in the inoculum container 10 is scraped. The motor 66 rotates in the reverse direction so as to return to the descending end at the time when it reaches the rising end that reaches the dimension. With this reverse rotation, the bacteria scraping blade 65 and the motor 66 move down and return together with the germ scraping screw shaft 64.

At this time, before and after the surface portion of the hardened and aged inoculum S in the inoculum container 10 is scraped, the inoculum surface scraping and removing mechanism 54 is used.
Is exposed to the hot air of the sterilizing apparatus 55, which is a heating means comprising a hot air generator, and is sterilized by heating together with the mouth 10A of the seed cell container 10.

Subsequently, as shown in FIG. 12, the inoculum container 10 set in an inverted state, four of which are set in the inoculum container mounting member 13, is one slide from the final end position. Pulled back along with the reciprocation movement of the member 57,
In this state, the next inoculum container 10 is a fungus scraping blade 65 provided in the inoculum surface scraping and removing mechanism 54 from the mouth 10A.
Similarly, only the surface portion of the hardened and aged inoculum S in the inoculum container 10 is scraped off and the cultivation container 1
The heat sterilization of the mouth portion 10A of No. 0 and the scraping blade 65 is performed, and the same operation is sequentially repeated as shown in FIGS.

Therefore, in this embodiment, in the pretreatment stage of the inoculation process by the inoculum inoculation device 11 described above, the return of the inoculum container pretreatment transport mechanism 56 causes the inoculation of the inoculated inoculum S in the inoculum container 10. A step of scraping only the surface portion, and a fungus scraping blade 6 which is a part of the inoculum surface scraping and removing mechanism 54 used at the time of scraping.
5 and the step of heating and sterilizing the vicinity of the mouth 10A of the inoculum container 10 can be performed in advance before inoculation of the inoculum, whereby the inoculum can be easily scraped off by simply setting the inoculum container 10 in an inverted state. There is an effect that it is possible to provide an inoculum inoculating apparatus capable of performing a sterilization treatment.

In this case, after the completion of the repetitive operation of the pretreatment process of the inoculum inoculation, the inoculum container 1 placed on the mounting slide member 57 and returned to the guide member 14 position
The seed container mounting member 13 with the “0” mounted in an upside down state is moved to the position where the seed S is scraped out of the seed inoculation device 11 by the release operation of the pretreatment process stopper provided on the mounting slide member 57. The support member 17 fixed to the piston descends with the lowering operation of the piston of the cylinder 16, and the positioning shaft 27 attached to the support member 17 and the pressing plate 20 move with the lowering operation. Then, the tip of the positioning shaft 27 is inserted into the fitting hole 28 provided in the inoculum container mounting member 13, and the bottom portions 10 </ b> B of the four inoculum containers 10 newly supplied by the pressing plate 20.
Is pressed and supported, thereby preparing for scraping out the seed bacteria S in the various bacteria containers 10, and then rotating the seed blade 33 and the seed bacteria container 10 to rotate the seed bacteria S as described above.
The seeding operation is sequentially performed, and the inoculation operation is sequentially performed by filling the inside of the cultivation container 6 with the inoculum S. Thereby, the inoculum container is set in the inoculum inoculation apparatus while the inoculum container 10 is set in an inverted state. Aging inoculum S in 10
And the sterilization of the vicinity of the mouth 10A of the seed container 10 and the sterilization of the bacteria scraping blade 65 that performs the bacterial scraping operation can be performed.

The present invention is not limited to the above-described embodiment, and various modifications can be made within the scope of the present invention. Was applied, but a sterilization mechanism including heating means such as an alcohol lamp and a gas burner may be used. Further, as a means that does not use heat, not limited to the heating means, for example, a sterilization treatment apparatus that sterilizes by spraying alcohol or a sterilizing chemical may be provided.

Although the inoculum scraping device of this embodiment is shown as an example in which the inoculum scraping device is integrally attached to the pre-process position of the inoculum inoculation device, it is individually configured as an inoculum scraping device that performs only the pre-treatment process. You may.

[0072]

According to the present invention, there is provided a seed bacterium mounting member for disposing a seed germ container in an inverted state, and a seed germ having a germ scraping blade for scraping a surface portion of an aged germ in the seed germ container mounted on the seed germ container mounting member. A surface scraping and removing mechanism, a sterilization apparatus including a heating means for heating and sterilizing the mouth of the seed container into which the bacteria scraping blade of the scraping and removing mechanism is inserted, and a seed container rotating mechanism for rotating the seed container. By having prepared,
By setting the inoculum container on the inoculum attachment member, it is possible to continuously perform sterilization work around the mouth of the inoculum container while easily scraping and removing the inoculum, and remove the surface of the aged inoculum. There is an effect that the scraped inoculum container can be sent to the inoculum inoculation device provided in the inoculum inoculation device.

In some cases, the surface scraping operation of the inoculum in the inoculum container is performed by disposing the inoculum scraping blade of the inoculum in the inoculum container and the sterilizing means in close proximity to each other. It is also possible to simultaneously sterilize the mouth portion of the seed cell container arranged in a state and the bacteria scraping blade of the seed cell surface scraping and removing mechanism.

In the present invention, since the heating means comprises a hot air generator, the apparatus does not use a fire such as an alcohol lamp or a burner as in the prior art, so that the apparatus can be easily handled.

[Brief description of the drawings]

FIG. 1 is a schematic perspective view showing the entire configuration of a seed inoculum inoculation apparatus applied in one embodiment of the present invention.

FIG. 2 is a main side view of the inoculum inoculation apparatus applied in one embodiment of the present invention.

FIG. 3 is a partial perspective view showing a main part of the inoculum inoculation device in the inoculum inoculation device applied in one embodiment of the present invention.

FIG. 4 is a front view of a main part of the inoculum inoculation apparatus applied to one embodiment of the present invention, showing a state where the inoculum container is released.

FIG. 5 is a front view of a main part when the seed container of FIG. 4 is set.

FIG. 6 is a schematic diagram showing a configuration of a pretreatment step of the inoculum inoculation apparatus applied to one embodiment of the present invention.

FIG. 7 is a main side view mainly showing a seed fungus scraping-out mechanism which is a pre-process in the seed fungus inoculating apparatus of FIG. 6;

FIG. 8 is a main side view showing the inoculum scraping-out mechanism and the sterilizing apparatus of FIG. 7;

FIG. 9 is a structural view of a hot air generator that is the sterilization apparatus of FIG.

FIG. 10 is an operation explanatory view of a pretreatment step in the inoculum inoculation apparatus applied to one embodiment of the present invention.

FIG. 11 is an operation explanatory view of a pretreatment step in the inoculum inoculation apparatus applied to one embodiment of the present invention.

FIG. 12 is an operation explanatory view of a pretreatment step in the inoculum inoculation apparatus applied to one embodiment of the present invention.

FIG. 13 is an operation explanatory view of a pretreatment step in the inoculum inoculation apparatus applied to one embodiment of the present invention.

FIG. 14 is an operation explanatory view of a pretreatment step in the inoculum inoculation apparatus applied to one embodiment of the present invention.

[Explanation of symbols]

 Reference Signs List 10 seed cell container 10A mouth 13 seed cell container mounting member 54 seed cell surface scraping and removing mechanism 55 sterilization treatment device 65 cell seed scraping blade 75 seed cell container rotating mechanism S seed cell

 ────────────────────────────────────────────────── ─── Continued on the front page (72) Inventor Masahiko Fujisawa 2-34 Higashizao, Nagaoka City, Niigata Prefecture Japan Seiki Co., Ltd.

Claims (4)

[Claims] 1. A seed bacterium scraper having a seed bacterium container mounting member for disposing a seed bacterium container in an inverted state, and a germ scraping blade for scraping a surface portion of an aged seed bacterium in the seed bacterium container mounted on the seed bacterium container mounting member. A take-off mechanism, a sterilization apparatus for sterilizing the mouth of the seed container into which the bacteria scraping blade is inserted, and a seed container rotating mechanism for rotating the seed container mounted on the seed container mounting member. An inoculum scraping device, characterized in that: 2. The inoculum scraping apparatus according to claim 1, wherein said sterilizing apparatus comprises a heating means. 3. The inoculum scraping apparatus according to claim 2, wherein said heating means comprises a hot air generator. 4. The inoculum scraping device according to claim 1, wherein the sterilization treatment device is provided adjacent to the inoculum scraping blade of the inoculum surface scraping and removing mechanism and sterilizes the inoculum scraping blade. .