JPH11174025A - Specimen feed device for electrophoresis analysis - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a specimen feed device for electrophoresis analysis capable of feeding specimen easily and accurately to a specimen feeding part when a vertical or horizontal gel is used. SOLUTION: A specimen solution feed part 2 made of a material capable of absorbing or absorbing the specimen solution reversibly is installed at the tip end or on the side surface of at least one tooth 1a of a comb-shaped supporting body 1 made of a material having a non-absorptivity to the specimen solution so as to form a specimen feed device for electrophoresis analysis. Also, specimen solution feed parts 2 made of the same material is installed on one major surface of a rectangular supporting body 1 made of the same material separately from each other in the direction in parallel with one side of the supporting body 1 so as to form the specimen feed device for electrophoresis analysis. In addition, at least one tooth 1a of the comb- shaped supporting body 1 made of filter paper is impregnated partially with wax so as to form the specimen solution feed part 2 at the tip end of the tooth 1a so as to form the specimen feed device for electrophoresis analysis. Then the rectangular supporting body 1 made of filter paper is impregnated partially with wax to form a plurality of specimen solution feed parts 2 separately from each other in the direction in parallel with one side of the supporting body 1 in order to form the specimen feed device for electrophoresis analysis.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a sample addition tool for electrophoresis analysis, and is particularly suitable for use in the analysis of proteins and nucleic acids by electrophoresis.

[0002]

2. Description of the Related Art An electrophoresis method is an analysis method that is widely used for analyzing proteins, nucleic acids, and the like. The analysis method using the electrophoresis includes a method using a vertical gel and a method using a horizontal gel.

Conventionally, as a method of adding a sample when performing electrophoretic analysis using a vertical gel, a sample hole is previously formed using a comb-shaped jig called a comb at the time of gel preparation, and the sample is added to a migration tank. A method in which a sample solution is added to the sample hole of the gel while the gel is fixed, or a gel without a sample hole is prepared, and a jig called a shark comb is inserted into the gel at the time of use to create the sample hole, and the electrophoresis is performed. There is a method in which a sample solution is added to a sample hole of the gel while the gel is fixed in a tank.

On the other hand, as a method of adding a sample when performing electrophoretic analysis using a horizontal gel, a method of preparing a sample hole using a comb in advance at the time of gel preparation, and adding a sample solution to the sample hole, Alternatively, there is a method in which a gel having no sample hole is prepared, filter paper pieces are placed one by one on the gel at the time of use, and a sample solution is added to the filter paper pieces.

[0005]

However, in the above-mentioned conventional sample addition method used when performing electrophoresis analysis using a vertical gel, the sample solution is filled into the sample hole of the gel fixed in the electrophoresis tank. Because of the addition, it was very difficult to add the sample and required skill.

[0006] In the above-described conventional sample addition method used for performing electrophoresis analysis using a horizontal gel, the sample solution is added to the sample hole of the gel fixed in the electrophoresis tank, so that the sample The addition was very difficult and required skill. Furthermore, in particular, in the method of placing the filter paper pieces one by one on the gel and adding the sample solution to the filter paper pieces, the operation itself of placing the filter paper pieces one by one is extremely complicated,
Variations occur in the positions where the filter paper pieces are placed, which may hinder the analysis.

[0007] Accordingly, an object of the present invention is to provide a sample addition tool for electrophoresis analysis which can easily and surely add a sample to a sample addition site when a vertical gel or a horizontal gel is used. Is to do.

[0008]

In order to achieve the above object, a sample adding device for electrophoretic analysis according to a first aspect of the present invention comprises a comb-shaped support made of a material that is non-absorbable with respect to a sample solution. And a sample solution addition section attached to at least one tooth tip or side surface of the support, and made of a material capable of reversibly adsorbing or absorbing the sample solution.

A sample adding device for electrophoretic analysis according to a second aspect of the present invention has a comb-shaped support made of filter paper, and at least one side of at least one tooth of the support is partially impregnated with wax to form a tooth. Is characterized in that a sample solution adding section is formed at the tip of the sample.

According to a third aspect of the present invention, there is provided a sample-adding device for electrophoretic analysis, comprising: a rectangular support made of a material that is non-absorbable with respect to a sample solution; and one side of the support on one main surface of the support. And a plurality of sample solution addition sections made of a material capable of reversibly adsorbing or absorbing a sample solution, which are attached separately from each other in a direction parallel to the above.

According to a fourth aspect of the present invention, there is provided a sample adding device for electrophoretic analysis having a rectangular support made of filter paper, wherein the support is partially impregnated with wax and is parallel to one side of the support. And a plurality of sample solution adding sections formed separately from each other.

In the first or third aspect of the present invention, the material of the support is polyethylene terephthalate (PET), polystyrene, acrylic resin, polycarbonate, polyvinyl chloride, polyethylene, polypropylene, polyamide (nylon), Polybutyl terephthalate, polyacetal, acrylonitrile / butadiene / styrene resin (ABS resin), acrylonitrile / styrene resin (AS resin), and the like can be used. As a material of the sample solution adding section, filter paper, glass fiber, cellulose, or the like can be used. Further, as a material of the sample solution adding section, polyamide (nylon), polycarbonate,
Polyethersulfone, polyethylene terephthalate (PET), polypropylene, polysulfone, polyurethane, or the like that has been subjected to a hydrophilic treatment by plasma discharge or hydrophilic photocatalyst coating can also be used.

In the first invention or the third invention of the present invention, typically, the sample solution adding section is adhered to the tips or side faces of the teeth of the support with an adhesive. As the adhesive, for example, a hot melt adhesive (such as an ethylene-vinyl acetate resin (EVA) -based adhesive) or an organic solvent-based adhesive (such as a vinyl chloride resin-based or synthetic rubber-based adhesive) may be used. This is particularly suitable when the material of the sample solution addition section is filter paper. Here, when an organic solvent-based adhesive is used, it is desirable to minimize the amount of use and to volatilize the organic solvent with a sufficient solidification time (for example, 2 to 3 days) after bonding. This is because the remaining organic solvent in the adhesive affects the results of the electrophoretic analysis. Further, instead of using an adhesive, the sample solution adding section may be ultrasonically welded to the tips or side faces of the teeth of the support.

In the second or fourth aspect of the present invention, synthetic wax (eg, polyethylene wax), petroleum wax (eg, paraffin wax), liquid paraffin, etc. can be used as the wax.

In the third or fourth aspect of the present invention, the sample solution addition sections are typically provided separately from each other in the longitudinal direction of the support.

The sample addition tool for electrophoresis analysis according to the first or second invention of the present invention is suitably used for, for example, nucleic acid or protein analysis. The sample addition device for electrophoresis analysis according to the third or fourth invention of the present invention is suitably used for, for example, protein analysis.

According to the sample addition device for electrophoretic analysis according to the first or second aspect of the present invention having the above-mentioned configuration, place it in a place where it is easy to work before setting it in the electrophoretic analyzer. Since the sample addition device for electrophoresis analysis can be placed and the sample solution can be added to the sample solution addition section, the sample addition operation can be performed easily and reliably.

According to the electrophoretic analysis sample adding tool of the third or fourth aspect of the present invention constructed as described above, the electrophoretic analysis should be carried out in a place which is easy to work before performing the electrophoretic analysis. Since the sample addition tool for analysis is placed and the sample solution can be added to the sample solution addition section, the sample addition operation can be performed easily and reliably.

[0019]

Embodiments of the present invention will be described below with reference to the drawings. In all the drawings of the embodiments, the same or corresponding portions are denoted by the same reference numerals. In the embodiment, the sample addition tool for electrophoresis analysis is referred to as a sample applicator for electrophoresis analysis.

FIG. 1 shows a vertical type sample applicator for electrophoretic analysis according to a first embodiment of the present invention. Here, FIGS. 1A, 1B and 1C are plan views of the sample applicator for electrophoresis analysis,
It is a side view and a perspective view.

As shown in FIG. 1, in the sample applicator for electrophoretic analysis according to the first embodiment, a plurality of teeth 1a of a comb-shaped support 1
The sample solution addition section 2 having the same width as that of the sample solution is adhered. Tooth 1a
The number is determined according to the number of lanes in which the electrophoresis analysis is performed, but here is eight as an example. Teeth 1 of support 1
The two corners opposite to a are rounded.

The support 1 is made of a material that does not absorb the sample solution to be used. Specifically, for example, PET, polystyrene, acrylic resin, polycarbonate, polyvinyl chloride, polyethylene, polypropylene, polyamide, polybutyl Terephthalate, polyacetal, AB
It is made of S resin, AS resin and the like. The sample solution adding section 2 is made of a material capable of reversibly adsorbing or absorbing the sample solution to be used, and specifically, is made of, for example, filter paper. Further, for example, an EVA hot melt adhesive is used for bonding the sample solution adding section 2 to the support 1.

To give an example of the dimensions of each part of the sample applicator for electrophoretic analysis, the length a is 30 to 40.
0 mm, width b is 20 to 70 mm, thickness c of the support 1 is 0.05 to 0.5 mm, thickness d of the sample solution adding section 2 is 0.1 to 2.0 mm, and width of the sample solution adding section 2 e is 1.0
１10.0 mm.

The sample applicator for electrophoretic analysis according to the first embodiment can be manufactured, for example, as follows. That is, first, one side of a filter paper is brought into close contact with one side of a film made of a material that is not absorbable with respect to a sample solution via an EVA-based hot melt adhesive. Next, in this state, the EVA hot melt adhesive is melted by heating to a temperature of, for example, 100 ° C. or more, and the film and the filter paper are bonded. Thereafter, these films and filter paper are cut into the shape shown in FIG. 1A. Thereby, the target sample applicator for electrophoretic analysis is manufactured.

Next, a method of performing electrophoretic analysis using the sample applicator for electrophoretic analysis according to the first embodiment configured as described above will be described.
Here, as an example, a case where a simple electrophoresis apparatus for measuring a DNA base sequence is used will be described.

First, a sample solution containing a sample to be subjected to electrophoretic analysis is taken, for example, with a pipette, and this sample solution is placed in FIG.
The required amount is added to the sample solution addition section 2 of the sample applicator for electrophoresis analysis shown in (1).

On the other hand, a gel cassette for electrophoresis analysis is prepared in advance according to the following procedure, and this gel cassette for electrophoresis analysis is set in an electrophoresis apparatus.

That is, as shown in FIG. 2, a rectangular plate 11 made of a rectangular glass plate and a notch plate 12 made of a glass plate of the same size and having a notch portion 12a at the upper end thereof. After sandwiching the elongated spacers 13 between the both side edges, the tapes are superposed on each other, and a waterproof tape (not shown) is attached to these side and bottom surfaces. Next, both side edges of the rectangle plate 11 and the notch plate 12 are clipped with clips (not shown). Next, these rectangle plate 11 and notch plate 12
A polyacrylamide gel is prepared by injecting a reagent solution into the gap between the two to cause a polymerization reaction to cause gelation. Next, after removing the clip, the waterproof tape is removed. FIG. 3 and FIG. 4 correspond to FIG.
FIG. 4 is a sectional view taken along line IV-IV. 3 and 4, reference numeral 14 denotes a polyacrylamide gel.

Next, as shown in FIG.
The lower side edges of the plate 11 and the notch plate 12 are sandwiched by two clips 15 on each side.

Next, as shown in FIG. 6, an upper buffer tank 16 made of an acrylic resin or a polycarbonate resin is attached to the notch portion 12a of the notch plate 12, and then both ends of the upper buffer tank 16 are notched. -Clip the plate 12 and the rectangle plate 11 with the clip 15. In this case, in order to prevent the buffer solution put in the upper buffer tank 16 from leaking,
As shown in FIG. 6, the gap between the upper buffer tank 16 and the notch plate 12 is sealed by a silicon rubber gasket 18 fitted in a groove 17 provided on the mounting surface of the upper buffer tank 16. .

Next, as shown in FIG. 7, the lower end of the gel cassette shown in FIG. 5 manufactured as described above is placed in the buffer solution 2 in the lower buffer tank 19 of the electrophoresis apparatus.
Put in 0.

Next, as shown in FIG. 8, each sample solution adding section 2 of the sample applicator for electrophoretic analysis shown in FIG.・ Rectangle plate 1 of cassette
1 and the upper surface of the polyacrylamide gel 14 sandwiched between the notch plate 12.

Next, as shown in FIG. 7, a buffer solution 21 is put into the upper buffer tank 16. Then, a predetermined voltage is applied between the buffer solution 20 in the lower buffer tank 19 as a positive electrode and the buffer solution 21 in the upper buffer tank 16 as a negative electrode to perform electrophoresis. After the electrophoretic separation is performed in this manner, the electrophoresis pattern is transferred to a film for radiography, and the DNA base sequence is read and analyzed from the electrophoresis pattern.

As described above, according to the sample applicator for electrophoretic analysis according to the first embodiment, the comb-shaped support 1 made of a material that is non-absorbable with respect to the sample solution.
When performing electrophoretic analysis by comprising a sample solution adding section 2 made of a material capable of reversibly adsorbing or absorbing a sample solution, which is adhered to the tip of each tooth 1a of the support 1 In this case, the sample applicator for electrophoresis analysis can be placed in an easy-to-work place, and the sample solution can be added to the sample solution addition section 2 of the sample applicator for electrophoresis analysis. Therefore, the operation of adding the sample solution can be performed extremely easily and reliably as compared with the conventional case. Further, the sample applicator for electrophoresis analysis has a simple structure, is excellent in mass productivity, and can be manufactured with inexpensive materials, so that the manufacturing cost is low.

FIG. 9 shows a vertical type sample applicator for electrophoretic analysis according to a second embodiment of the present invention. 9A, 9B and 9C are plan views of the sample applicator for electrophoresis analysis,
It is a side view and a perspective view.

As shown in FIG. 9, in the sample applicator for electrophoretic analysis according to the second embodiment, one end of a plurality of teeth 1a of a comb-shaped support 1 has the same width as the teeth 1a. The sample solution adding section 2 is bonded. Other points are the same as those of the sample applicator for electrophoresis analysis according to the first embodiment, and the description is omitted.

The sample applicator for electrophoretic analysis according to the second embodiment can be manufactured, for example, as follows. That is, first, a filter paper is brought into close contact with one main surface of a film made of a material that does not absorb a sample solution via an EVA-based hot melt adhesive so as to partially overlap the film. Next, in this state, the EVA hot melt adhesive is melted by heating to a temperature of, for example, 100 ° C. or more, and the film and the filter paper are bonded. Thereafter, these films and filter paper are cut into the shape shown in FIG. 1A. Thereby, the target sample applicator for electrophoretic analysis is manufactured.

Here, when PET is used as the material of the support 1, a PET film coated with an EVA film (commercially available as a laminating film) can be used more easily for electrophoresis analysis. It is possible to manufacture a sample applicator.

The method of using the sample applicator for electrophoresis analysis according to the second embodiment is the same as that of the sample applicator for electrophoresis analysis according to the first embodiment, and a description thereof will be omitted.

According to the second embodiment, advantages similar to those of the first embodiment can be obtained.

FIG. 10 shows a vertical type sample applicator for electrophoretic analysis according to a third embodiment of the present invention. Here, FIGS. 10A, 10B, and 10C are a plan view, a side view, and a perspective view of the sample applicator for electrophoresis analysis, respectively.

As shown in FIG. 10, in the sample applicator for electrophoretic analysis according to the third embodiment, a plurality of teeth 1a of a comb-shaped support 1 made of filter paper are traversed across the teeth 1a. The wax-impregnated portion 3 is provided in the form of a strip as shown in FIG.
A sample solution addition section 2 is formed in a portion between the tip and the tip a. The number of the teeth 1a is selected according to the number of lanes in which the electrophoresis analysis is performed. Here, the number is, for example, eight. The two corners of the support 1 opposite to the teeth 1a are rounded.

As the wax to be impregnated in the wax impregnating section 3, for example, synthetic wax such as polyethylene wax is used.

As an example of the dimensions of each part of the sample applicator for electrophoretic analysis, the length a is 30 to 40.
0 mm, width b is 20 to 70 mm, thickness c of the support 1 is 0.1 to 2.0 mm, and width e of the sample solution adding section 2 is 1.0.
１10.0 mm.

The sample applicator for electrophoretic analysis according to the third embodiment can be manufactured, for example, as follows. That is, first, a strip of wax-impregnated paper having the same width as the wax-impregnated portion 3 is placed on the filter paper.
Next, by heating in this state, the wax is impregnated into the filter paper from the wax-impregnated paper to form the wax-impregnated portion 3. Thereafter, the filter paper is cut into the shape shown in FIG. 10A. Thereby, the target sample applicator for electrophoretic analysis is manufactured.

The method of using the sample applicator for electrophoresis analysis according to the third embodiment is the same as that of the sample applicator for electrophoresis analysis according to the first embodiment, and a description thereof will be omitted.

According to the third embodiment, advantages similar to those of the first embodiment can be obtained.

FIG. 11 shows a vertical type sample applicator for electrophoretic analysis according to a fourth embodiment of the present invention. Here, FIGS. 11A, 11B, and 11C are a plan view, a side view, and a perspective view of the sample applicator for electrophoresis analysis, respectively.

As shown in FIG. 11, in the sample applicator for electrophoretic analysis according to the fourth embodiment, the entire portion of the comb-shaped support 1 made of filter paper except for the tips of the teeth 1a is formed. A wax impregnating section 3 is provided, and a sample solution adding section 2 is formed at the tip of each tooth 1a. Others are the same as the sample applicator for electrophoresis analysis according to the third embodiment,
Description is omitted.

The method of manufacturing and using the sample applicator for electrophoretic analysis according to the fourth embodiment is the same as that of the sample applicator for electrophoretic analysis according to the first embodiment, and a description thereof will be omitted.

According to the fourth embodiment, advantages similar to those of the first embodiment can be obtained.

FIG. 12 shows a horizontal type sample applicator for electrophoretic analysis according to a fifth embodiment of the present invention. Here, FIG. 12A and FIG. 12B are a plan view and a side view of the sample applicator for electrophoresis analysis, respectively.

As shown in FIG. 12, in the sample applicator for electrophoretic analysis according to the fifth embodiment, one of the elongated rectangular supports 1 is separated from each other in the longitudinal direction of the supports 1. Then, a rectangular sample solution addition section 2 having the same width as the support 1 is adhered. The number of the sample solution adding sections 2 is selected according to the number of lanes in which the electrophoretic analysis is performed. Here, the number is, for example, eight. The four corners of the support 1 are rounded.

The materials of the support 1 and the sample solution adding section 2 and the adhesive used for bonding them are the same as those of the sample applicator for electrophoresis analysis according to the first embodiment, and therefore the description is omitted.

As an example of the dimensions of each part of the sample applicator for electrophoretic analysis, the length a is 30 to 40.
0 mm, width b is 2.0 to 15.0 mm, thickness c of the support 1 is 0.05 to 0.5 mm, and thickness d of the sample solution adding section 2
Is 0.1 to 2.0 mm.

The method of manufacturing the sample applicator for electrophoresis analysis according to the fifth embodiment is the same as that of the sample applicator for electrophoresis analysis according to the first embodiment, and a description thereof will be omitted.

In order to perform electrophoretic analysis using the sample applicator for electrophoretic analysis according to the fifth embodiment configured as described above, the sample for electrophoretic analysis must be placed on the sample addition site on the horizontal gel. -The electrophoresis may be performed by placing the applicator so that the sample solution adding section 2 faces down.

According to the fifth embodiment, advantages similar to those of the first embodiment can be obtained.

FIG. 13 shows a horizontal type sample applicator for electrophoretic analysis according to a sixth embodiment of the present invention. 13A and 13B are a plan view and a side view of the sample applicator for electrophoresis analysis, respectively.

As shown in FIG. 13, in the sample applicator for electrophoretic analysis according to the sixth embodiment, one of the elongated rectangular supports 1 is separated from each other in the longitudinal direction of the supports 1. Then, a rectangular sample solution adding section 2 narrower than the support 1 is adhered. The other points are the same as those of the sample applicator for electrophoresis analysis according to the fifth embodiment, and the description is omitted.

The method of manufacturing and using the sample applicator for electrophoretic analysis according to the sixth embodiment is the same as that of the sample applicator for electrophoretic analysis according to the fifth embodiment, and therefore, the description thereof is omitted.

According to the sixth embodiment, advantages similar to those of the first embodiment can be obtained.

FIG. 14 shows a horizontal type sample applicator for electrophoretic analysis according to a seventh embodiment of the present invention. 14A and 14B are a plan view and a side view of the sample applicator for electrophoresis analysis, respectively.

As shown in FIG. 14, in the sample applicator for electrophoretic analysis according to the seventh embodiment, an elongated rectangular support 1 made of filter paper is separated from each other in the longitudinal direction of the support 1. And the support 1
A strip-shaped wax-impregnated portion 3 is provided so as to cross the section, and a sample solution adding portion 2 is formed between the wax-impregnated portions 3. The number of sample solution addition sections 2 is selected according to the number of lanes in which the electrophoresis analysis is performed.
Here, the number is eight as an example. The four corners of the support 1 are rounded.

As the wax to be impregnated in the wax impregnating section 3, for example, a synthetic wax such as polyethylene wax is used.

As an example of the dimensions of each part of the sample applicator for electrophoretic analysis, the length a is 30 to 40.
0 mm, width b is 2.0 to 15.0 mm, and thickness c of the support 1 is 0.1 to 2.0 mm.

The method of manufacturing the sample applicator for electrophoretic analysis according to the seventh embodiment is the same as the method of manufacturing the sample applicator for electrophoretic analysis according to the third embodiment, and a description thereof will be omitted. The method of using the sample applicator for electrophoresis analysis according to the seventh embodiment is the same as the method of manufacturing the sample applicator for electrophoresis analysis according to the fifth embodiment, and a description thereof will not be repeated.

According to the seventh embodiment, advantages similar to those of the first embodiment can be obtained.

FIG. 15 shows a horizontal type sample applicator for electrophoretic analysis according to the eighth embodiment of the present invention. Here, FIGS. 15A and 15B are a plan view and a side view of the sample applicator for electrophoresis analysis, respectively.

As shown in FIG. 15, in the sample applicator for electrophoretic analysis according to the eighth embodiment, an elongated rectangular support 1 made of filter paper is separated from each other in the longitudinal direction of the support 1, A wax impregnated portion 3 is provided except for a rectangular region having a width narrower than that of the support 1, and a sample solution adding portion 2 is formed in a portion surrounded by the wax impregnated portion 3. The other points are the same as those of the sample applicator for electrophoresis analysis according to the seventh embodiment, and the description is omitted.

The method for manufacturing the sample applicator for electrophoresis analysis according to the eighth embodiment is the same as the method for manufacturing the sample applicator for electrophoresis analysis according to the third embodiment, and a description thereof will be omitted. The method of using the sample applicator for electrophoresis analysis according to the eighth embodiment is the same as the method of manufacturing the sample applicator for electrophoresis analysis according to the fifth embodiment, and a description thereof will be omitted.

According to the eighth embodiment, advantages similar to those of the first embodiment can be obtained.

Although the embodiments of the present invention have been specifically described above, the present invention is not limited to the above embodiments, and various modifications based on the technical concept of the present invention are possible.

For example, the numerical values, structures, shapes, manufacturing methods, and the like described in the above-described first to eighth embodiments are merely examples, and different numerical values, structures, shapes, manufacturing methods, and the like may be used as necessary. May be used.

Specifically, in some cases, in the above-described second, fifth, or sixth embodiment, the sample solution addition section 2 may be bonded to the support 1 using a double-sided tape.

In the above-described first to eighth embodiments, the corners of the support 1 are rounded. However, the corners need not always be rounded, and may be right angles.

[0077]

As described above, according to the sample addition tool for electrophoresis analysis according to the present invention, when a vertical gel or a horizontal gel is used, the sample can be easily and reliably placed at the sample addition site. Can be added.

[Brief description of the drawings]

FIG. 1 is a plan view, a side view, and a perspective view of a vertical type sample applicator for electrophoretic analysis according to a first embodiment of the present invention.

FIG. 2 is a perspective view of the gel cassette for electrophoresis analysis used in the first embodiment of the present invention, as viewed from a notch plate side.

FIG. 3 is a sectional view taken along the line III-III in FIG. 2;

FIG. 4 is a sectional view taken along line IV-IV in FIG. 2;

FIG. 5 is a perspective view for explaining a method of using the gel cassette for electrophoresis analysis used in the first embodiment of the present invention.

FIG. 6 is a perspective view showing an upper buffer tank attached to a notch plate of the gel cassette for electrophoresis analysis used in the first embodiment of the present invention.

FIG. 7 is a cross-sectional view for explaining a method for performing electrophoretic analysis in the first embodiment of the present invention.

FIG. 8 is a cross-sectional view for explaining a method of mounting a sample applicator for electrophoresis analysis on a gel cassette for electrophoresis analysis used in the first embodiment of the present invention.

FIG. 9 is a plan view, a side view, and a perspective view of a vertical type sample applicator for electrophoresis analysis according to a second embodiment of the present invention.

FIG. 10 is a plan view, a side view, and a perspective view of a vertical type sample applicator for electrophoresis analysis according to a third embodiment of the present invention.

FIG. 11 is a plan view, a side view, and a perspective view of a vertical type sample applicator for electrophoretic analysis according to a fourth embodiment of the present invention.

FIG. 12 is a plan view and a side view of a horizontal type sample applicator for electrophoretic analysis according to a fifth embodiment of the present invention.

FIG. 13 is a plan view and a side view of a horizontal type sample applicator for electrophoretic analysis according to a sixth embodiment of the present invention.

14A and 14B are a plan view and a side view of a horizontal type sample applicator for electrophoretic analysis according to a seventh embodiment of the present invention.

FIG. 15 is a plan view and a side view of a horizontal type sample applicator for electrophoretic analysis according to an eighth embodiment of the present invention.

[Explanation of symbols]

DESCRIPTION OF SYMBOLS 1 ... Support, 1a ... Teeth, 2 ... Sample solution addition part, 3 ... Wax impregnation part, 11 ... Rectangle plate, 12 ... Notch plate, 14 ...
Polyacrylamide gel, 16: upper buffer tank, 19: lower buffer tank

Claims (18)

[Claims] 1. A comb-shaped support made of a material that is non-absorbable with respect to a sample solution; and a reversibly adsorbing or absorbing said sample solution attached to a tip or a side of at least one tooth of said support. A sample solution addition section made of a possible material. 2. The support is made of polyethylene terephthalate, polystyrene, acrylic resin, polycarbonate,
The electrophoretic analysis sample addition tool according to claim 1, wherein the tool is made of polyvinyl chloride, polyethylene, polypropylene, polyamide, polybutyl terephthalate, polyacetal, acrylonitrile-butadiene-styrene resin, or acrylonitrile-styrene resin. 3. The sample addition device for electrophoretic analysis according to claim 1, wherein the sample solution addition section is made of filter paper, glass fiber or cellulose. 4. The sample solution adding section includes a hydrophilically treated polyamide, polycarbonate, polyether sulfone, polyethylene terephthalate, polypropylene,
2. The sample addition device for electrophoretic analysis according to claim 1, wherein the device is made of polysulfone or polyurethane. 5. The sample addition tool for electrophoretic analysis according to claim 1, wherein the sample solution addition section is adhered to a tip or a side face of a tooth of the support with an adhesive. 6. The sample addition tool for electrophoretic analysis according to claim 5, wherein the adhesive is a hot melt adhesive or an organic solvent-based adhesive. 7. The sample addition tool for electrophoretic analysis according to claim 1, wherein the sample solution addition section is ultrasonically welded to a tip or a side face of a tooth of the support. 8. A comb-shaped support made of filter paper, wherein a side of at least one tooth of the support is partially impregnated with wax, and a sample solution adding section is formed at a tip of the tooth. A sample addition tool for electrophoresis analysis, characterized in that: 9. The sample loading device for electrophoretic analysis according to claim 8, wherein the wax is a synthetic wax, petroleum wax or liquid paraffin. 10. A rectangular support made of a material that is non-absorbable with respect to a sample solution, and separately attached on one main surface of the support in a direction parallel to one side of the support. And a plurality of sample solution addition sections made of a material capable of reversibly adsorbing or absorbing the sample solution. 11. The support comprises polyethylene terephthalate, polystyrene, acrylic resin, polycarbonate, polyvinyl chloride, polyethylene, polypropylene, polyamide, polybutyl terephthalate, polyacetal, acrylonitrile / butadiene / styrene resin, or acrylonitrile / styrene resin. The sample addition tool for electrophoresis analysis according to claim 10, characterized in that: 12. The method according to claim 1, wherein the sample solution adding section is made of filter paper, glass fiber or cellulose.
0. The sample loading device for electrophoretic analysis according to 0. 13. The electrophoretic analysis method according to claim 10, wherein the sample solution adding section is made of a hydrophilically treated polyamide, polycarbonate, polyether sulfone, polyethylene terephthalate, polypropylene, polysulfone or polyurethane. Sample addition tool. 14. The sample addition tool for electrophoresis analysis according to claim 10, wherein the sample solution addition section is adhered to the one main surface of the support with an adhesive. 15. The adhesive according to claim 14, wherein the adhesive is a hot melt adhesive or an organic solvent-based adhesive.
The sample addition device for electrophoresis analysis according to the above. 16. The sample addition tool for electrophoretic analysis according to claim 10, wherein the sample solution addition section is ultrasonically welded to a tip or a side face of a tooth of the support. 17. A rectangular support made of filter paper, wherein the support is partially impregnated with wax and separated from each other in a direction parallel to one side of the support to form a plurality of sample solution addition sections. A sample addition tool for electrophoresis analysis, characterized in that it is made. 18. The sample loading device for electrophoretic analysis according to claim 17, wherein the wax is a synthetic wax, petroleum wax or liquid paraffin.