JPH10338641A - Extraction of propolis extract - Google Patents

Abstract

PROBLEM TO BE SOLVED: To increase the extraction rate of propolis extract to more than that in the past by accepting the water-ethanol mixture extraction in the extraction of propolis extract and adjusting the ratio between water and ethanol in this case to the prescribed region. SOLUTION: The method is carried out by finely pulverizing propolis raw mass and then adding a mixture between ethanol and water containing water in a proportion of 10-35 vol.% under stirring to extract propolis. Next, the resultant mixture is centrifuged to separate the propolis extract and the top clear layer in the separated solution is filtered.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

The present invention relates to a method for extracting a propolis extract, and more particularly to a method for extracting a propolis extract using a mixed solution of ethanol and water.

[0002]

2. Description of the Related Art Propolis is a colloidal substance collected from bee sap and flower pollen by bees, and its bactericidal action and various pharmacological actions have been confirmed, and it is expected to be used in various applications.
However, since propolis is collected by bees in nature, it contains many impurities other than the active ingredient. Therefore, it is widely practiced to extract and use extracts from propolis.

[0003] As a method of extracting an active ingredient extract from propolis, a method of extracting ethanol from a propolis raw mass using ethanol as a solvent has been mainly used.
Although this method is excellent in extraction efficiency and excellent, the obtained propolis extract has a strong smell and a considerable irritation in taste. In addition, it has been pointed out that when dripping water into this extract, the insoluble matter floating on the water surface adheres to the inside of the container.

[0004] When extracting an extract from propolis, extraction using a mixture of ethanol and water has already been performed in order to increase the extraction rate. According to this method, the extraction rate is further increased, and the water-soluble components in the propolis are simultaneously extracted to obtain an excellent propolis extract.

[0005] Further, extraction of propolis with water alone is also a known technique. In this method, for example, a raw propolis mass is made into a fine powder, and water is added thereto, followed by stirring at 50 ° C for several hours. The amount of water to be added is, for example, 500 ml of water per 100 g of the original propolis mass. The extract is centrifuged to remove the supernatant, and the remaining precipitate is treated in the same manner.
The supernatant collected in the first and second treatments is filtered, and the filtrate is freeze-dried to obtain a powder of propolis extract (August 1, 1996, published by Toyo Medical School Co., Ltd., Polopolis Health Reader 1, 51 pages).

[0006] However, the conventional extraction methods of propolis extract with ethanol alone, water alone and water-ethanol mixture all have low extraction rates.
That is, the extraction ratio of propolis extract with ethanol alone was 28%, and the extraction ratio of propolis extract with water alone was 7%.
The extraction rate of water-ethanol mixture was about 30% in the publication of the literature. The raw propolis collected by honey from Brazilian eucalyptus forests is a valuable raw material, and it has been strongly desired to improve the extraction rate of propolis extract using this material. No method was found.

[0007]

SUMMARY OF THE INVENTION The present invention employs a water-ethanol mixture extraction in the extraction of a propolis extract, and the water / ethanol ratio at this time falls within a predetermined range to thereby reduce the extraction rate of the propolis extract. It is going to rise to no extent.

[0008]

According to the present invention, an original propolis mass is pulverized, and then a mixed solution of ethanol and water having a water ratio of 10 to 35% by volume is added thereto and stirred to extract propolis. Then, the propolis extract is separated by centrifuging the mixed solution to separate a propolis extract, and the supernatant of the separated solution is filtered. To the residue obtained by filtering the supernatant, a mixed solution of ethanol and water with a water ratio of 10 to 35% by volume was added and stirred to extract propolis. Then, the mixed solution was centrifuged to separate the propolis extract. A method for extracting a propolis extract, wherein the supernatant is separated and the supernatant of the separated liquid is filtered (claim 2). A method for extracting the propolis extract of claim 1, wherein the Police (claim 3).

[0009]

DETAILED DESCRIPTION OF THE INVENTION The starting material for this invention is a raw propolis mass, preferably from Brazil, collected primarily from honey from eucalyptus forests. This original block is visually cut in advance to remove foreign substances such as wood chips and stones, and cut into pieces of about 5 mm square with scissors. This is then pulverized with a pulverizer. If heat is generated during the pulverization, the propolis becomes lump due to the melted wax, and in that case, water is circulated through the jacket in the pulverizer to prevent the propolis from generating heat.

[0010] The crushed propolis is sieved for 20 minutes.
Let it be the granularity of the mesh pass. The pulverized propolis is put into a vessel equipped with a stirrer, and a mixed solution of ethanol and water is added, followed by stirring for a certain time to extract propolis.
The water ratio of the extract used here is 10 to 30% by volume, preferably 10 to 27.5% by volume. The most preferred water ratio is 25% by volume. That is, 90 volumes of ethanol are added.
７０70%, and water １０〜10-30%.

According to a number of experiments by the inventor, extraction of propolis extract with a mixture of ethanol and water is shown in FIG.
It has been found that there is a relationship as shown in FIG. That is, as shown in FIG. 1, it has been found that the propolis extract is more efficiently extracted by setting the ethanol ratio in the range of 90 to 30% by volume and the water ratio in the range of 10 to 70%. It is a thing. The range of the ethanol-water ratio in which the extraction of the propolis extract is performed more favorably is 10 to 27.5% by volume as seen in FIG. The highest extraction rate is obtained in the case of ethanol-water mixed extraction in which the ratio of ethanol to water is 75: 25% by volume.

[0012] The amount of the ethanol-water mixed solution to be put into the stirring vessel is preferably about 2.5 times the fine powder of propolis.
00 ml. Then, for example, at 320 rpm rotation,
Stir for an hour to perform the extraction. The stirring time is in the range of 1 to 2.5 hours. The extract may be kept at normal temperature, but may be heated to about 50 ° C.

After the completion of stirring, the extract is centrifuged to collect the extract. The centrifuge is, for example, 12,
Apply for 12 minutes at 000 rpm. Next, the supernatant liquid is filtered through a paper filter.

Further, an extract similar to the above is added again to the residue obtained by filtering the supernatant, and the mixture is stirred to elute the propolis extract. Thereafter, the solution is centrifuged to collect the supernatant, and the supernatant is collected. Similarly, it may be filtered. In the present invention, such secondary extraction is also included.

The propolis extract thus obtained is then bottled or the like to obtain a product. The extraction rate of the propolis extract here can be greatly increased by using a mixed solution of the above ratio of ethanol water.

The extraction ratio of propolis extract herein was determined according to a voluntary standard set by the Japan Propolis Extract Association. That is, 20 ml of a sample was cooled at -10 ° C or lower for 24 hours, and this was filtered with Advantech's filter paper No. 5C.
Cold filter using (1 micron). The filtrate was returned to room temperature, and 2 ml of the filtrate was collected, dried at 105 ° C. for 2 hours, allowed to cool for 30 minutes, weighed, and weighed, and the percent (V / V
W (%)) was determined by the following equation. Extraction rate (%) = {dry matter weight (g) / 2 ml} × 100

[0017]

【Example】

(Example 1) An original propolis lump collected from honey from a eucalyptus forest from Brazil was crushed to 5 mm square with scissors. 2000 g of the crushed propolis and 5000 ml of an ethanol-water mixed extract shown in Table 1 were mixed with a 10-liter stirrer (sample mill SKM-1 manufactured by Kyoritsu Riko Co., Ltd.).
0) and stirred at 320 rpm for 2 hours. The mixing ratio of ethanol and water in the ethanol-water mixed extract used was as shown in Table 1.

Next, the mixed stirring liquid was rotated at 12,
The mixture was centrifuged at 2,000 rpm for 12 minutes, and the supernatant was filtered with a paper filter to obtain a propolis extract. The extraction rate of the propolis extract at that time was as shown in Table 1. The extraction rate was measured by the method described above.

[0019]

[Table 1] Comparative Example 1 A propolis extract was obtained in the same manner as in Example 1. However, various mixing ratios of ethanol and water in the ethanol-water mixed extract shown in Table 2 outside the range specified in the present invention were used. The extraction rate of the propolis extract in this case was as shown in Table 2. The extraction rate was measured in the same manner as in the example.

[0020]

[Table 2]

[0021]

As described above, according to the present invention, in the extraction of a mixed solution of ethanol and water, the extraction ratio of propolis extract can be greatly increased by setting the ratio of ethanol to water within a predetermined range. became. Furthermore, in the present invention, since extraction is performed with an ethanol-water mixed solution, the obtained propolis extract can be a propolis extract containing both an ethanol-soluble extract and a water-soluble extract, and this can be efficiently produced at once. Is now available.

[Brief description of the drawings]

FIG. 1 is a diagram showing the relationship between the water volume ratio (%) of an extract and the extraction rate of a propolis extract.

Claims (3)

[Claims] 1. An original propolis mass is pulverized, and then a mixed solution of ethanol and water with a water ratio of 10 to 35% by volume is added thereto and stirred to extract propolis, and then the mixed solution is centrifuged. A method for extracting a propolis extract, comprising separating a propolis extract by a machine and filtering the supernatant of the separated solution. 2. A propolis is extracted by adding a mixed solution of ethanol and water having a water ratio of 10 to 35% by volume to the residue obtained by filtering the supernatant liquid according to the invention of claim 1 and stirring to extract propolis. A method for extracting a propolis extract, comprising subjecting a mixed solution to a centrifugal separator to separate a propolis extract, and further filtering a supernatant of the separated solution. 3. The method for extracting a propolis extract according to claim 1, wherein the original propolis mass is propolis obtained by collecting honey from a eucalyptus forest of Brazil.