JPH10327771A - Feed additive for fish and its use - Google Patents

Abstract

PROBLEM TO BE SOLVED: To remove the difference between natural fish and cultured fish by the functions of fossil shell to improve the nature of blood and control the color development of aquarium fish. SOLUTION: Fossil shell consisting of humus-soluble crystal produced by the embedding and deposition of various nektons, plankton, algae, seaweed, etc., composed of calcareous material, silicic acid, etc., is heat-treated at 150-300 deg.C to effect the removal of crystal water and the activation of the fossil to obtain a heat-treated fossil shell. The administration of the heat-treated fossil shell to fish for a prescribed period as a mixture with a fish feed is extremely effective as a blood nature improving agent and a color-development adjusting agent for aquarium fish to minimize the difference between natural fish and cultured fish.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a feed additive for fish and a method of using the same, and more particularly, to a heat treatment of natural shell fossils, a blood property improving function of the heat-treated shell fossils, and a color adjusting function of ornamental fish. The present invention relates to a fish feed additive that exclusively utilizes a unique property and a method of using the same.

[0002]

2. Description of the Related Art As a conventional feed additive for fish mainly containing shell fossils, Japanese Patent Application Laid-Open No. 63-196234 by the present applicant is known. According to this publication, 0.01 to 5
When 0.5 to 2% by weight of a shell fossil pulverized and refined to 0.00 μm is added to a fish feed and given to cultured fish, odor is eliminated, fat is reduced due to excessive nutrition due to lack of exercise, and body weight is reduced. Elimination of cracking phenomena, mortality reduction due to strengthening of constitution,
Further, it is disclosed that the environment of the farm is improved.

Further, Japanese Patent Application Laid-Open No. 7-3130 by the present applicant
According to No. 14, when cultivating marine animals and plants, natural shell fossils are heat-treated into granular shell fossils, and a predetermined amount is sprayed on the bottom of the cultivation site, and further, the heat-treated granular shell fossils are powdered. With respect to the donation of marine animals and plants.
It is disclosed that when 0% by weight is added to aquatic animals and plants, by the action of the components of these shell fossils, cultured aquatic animals and plants comparable to natural products can be grown while preparing the environment of the aquaculture site.

[0004]

All of the above-mentioned prior art examples by the present applicant are used for aquaculture by adding heat-treated shell fossils at a predetermined ratio to fish and animal donations such as fish feed. Despite the fact that the environment of the place is not desirable for the breeding of marine animals and plants, the use of the adsorption performance of heat-treated shell fossils and the replenishment of the minerals of the heat-treated shell fossils allow the production of cultured aquatic animals and plants comparable to natural products. It is a breakthrough because it can be raised. However, in these conventional examples, it is only described that the addition of heat-treated shell fossils to fish feed for a certain period of time to marine animals and plants resulted in a favorable effect. Not disclosed.
For this reason, the efficient administration time and period of the heat-treated shell fossil to be mixed with fish feed are unknown. Therefore, it is more difficult to narrow the gap between so-called natural products and cultured products, or to remove the barriers.

[0005] In general, the environment of aquaculture sites is overcrowding of aquatic animals and plants, so that it can be said that all inconveniences for aquatic animals and plants occur, and that the aquaculture sites are easily contaminated and lack of oxygen and minerals. Easy to be,
In the case of marine animals, the amount of exercise tends to be low and stress is likely to occur. Conversely, the advantage of marine animals and plants is that food and nutrients are automatically provided and there is little competition for survival. And overcrowding is something that cannot be avoided as long as it is aquaculture.

[0006] If this overgrowth is unavoidable, as a next step, the heat-treated shell fossils administered to the cultured aquatic animals and plants are given specific examples of the function of the cultured shellfish, which is comparable to natural products. It is necessary to clarify whether marine animals and plants can be raised. In other words, elucidate how heat-treated shell fossils act on the physiological mechanisms of cultured aquatic animals and plants to produce cultured aquatic animals and plants that are comparable to natural products, and what the health condition is at that time. It must be.

[0007] Based on the knowledge that the human health condition can be almost determined by examining the blood properties, the blood properties of natural products and cultured products are examined and the difference is grasped. It is necessary to examine how the heat-treated shellfish works on the physiological mechanism of cultured aquatic animals and plants by examining the change in blood properties caused by giving and comparing with the difference in blood properties between the above natural products and cultured products. There is.

Accordingly, the present invention has been made in view of the above circumstances, and among the effects of heat-treated shell fossils on the physiological mechanisms of aquatic animals and plants, the functions of improving blood properties and, in the case of ornamental fish, its coloring control function. Focused attention, clarified the effective administration time and period of heat-treated shellfish fossils, reduced the difference between so-called natural products and cultured products, and removed the barriers between them and fish feed additives and their use The task is to provide

[0009]

The present inventors have been investigating the composition and properties of shell fossils for many years. Based on the results obtained from the research, they found new uses and disclosed them each time. In addition, we have been conducting diligent research to find new uses for shell fossils. As a result, the present inventors reduced the muddy odor of the cultured carp and provided the cultured carp with a survival time (approximately 2. 6 hours) was dramatically extended (about 6.2 hours), far exceeding the conventional wisdom. That is, since the increase in carp's resistance to hypoxia implies that blood properties have been dramatically improved, the effects of shell fossils on carp's blood properties were investigated over a long period of time.
As a result, they found that the number of red blood cells, hemoglobin concentration, and hematocrit increased, and that blood properties were dramatically improved, and the present invention was reached.

That is, in order to solve the above-mentioned problems, the invention of claim 1 provides a humus-soluble crystal in which various nekton, plankton, algae, seaweed, and the like made of calcareous or silicic acid are buried and deposited. 150 貝 C ~ 3
A fish feed additive characterized in that heat treatment is performed within the range of 00 ° C to remove water of crystallization and activate to form a heat-treated shell fossil, and the heat-treated shell fossil is exclusively used as a blood property improving agent.

The shell fossils used in the present invention are foraminiferal fossils in the archeological name and calcareous sandstone in the geological name. In Japan, Toyama, Ishikawa, Noto Peninsula, Takayama, Gifu, Hokkaido, Yamaguchi, Tokushima It is produced in prefectures, Fukushima prefecture and Kagoshima prefecture, but there is no limitation depending on the place of production. Shell fossils from any locality may be used as long as the shell fossils have the following characteristics. Analytical values of shell fossils in the main production areas are as shown in Table 1.

More specifically, the fossil shellfish of the present invention is based on the following quantitative analysis table (Table 2) for a sample mined at a mining site in Toyama prefecture, and a mining sample in the same Toyama prefecture near the mining site. These are based on the following quantitative analysis table (Table 3) for the samples mined at the site, and similar products of the shell fossils of the components shown in Tables 2 and 3 mined from these mines.

[0013]

[Table 1]

[0014]

[Table 2]

[0015]

[Table 3]

The shell fossils mined in Toyama and Ishikawa prefectures differ greatly in the composition and molecular assemblage of shell fossils mined in other parts of Japan. It is characterized by a high calcium content and a good balance of the mixing ratio of silicon and calcium carbonate. And since these shell fossils were living organisms, they did not completely fossilize, they were deposited as they were, and were made by long-term compaction,
It contains trace elements necessary for living organisms, that is, essential minerals with high density and good balance. Table 4 shows the measurement results of the trace elements.

[0017]

[Table 4]

The fossil shell has an aragolite-type crystal structure secreted from a living body, has a certain effective diameter, and has a myriad of small pores containing water of crystallization. That is, it has an adsorption performance.

In order to obtain a heat-treated shell fossil obtained by imparting adsorption performance to a shell fossil mined from a mine, the shell fossil is crushed to 5 mm or less, passed through a mesh sieve having an opening of 5 mm, and dried with a drier. Heat treatment is performed within the range of ゜ C to 300 ° C. to remove water of crystallization, activate the mixture, pass through a 2 mm mesh mesh sieve, and cool to a normal temperature with a cooler to obtain.
After that, the heat-treated shell fossils are crushed by a known method to determine the particle size according to the purpose of use. The blood property improving agent preferably has a particle size of 75 μm or less, but is not particularly limited.

The blood property improving agent containing the heat-treated shellfish fossil as a main component of the present invention is given to fish by mixing the powdered product with fish feed. Since it is a blood property improving agent, it must be given to fish for a certain period of time, for example, for 1 to 2 months. The properties as a blood property improving agent include the following. -The erythrocyte count of fish showed an improvement tendency 2 weeks after administration, and a clear significant difference was recognized after 60 days. The red blood cells carry oxygen and are related to the total volume occupied by the red blood cells. If the total volume is constant, the red blood cells are small, and the larger the number, the larger the total surface area, which is convenient. -The hematocrit value showed a tendency to improve 2 weeks after administration, and a clear significant difference was recognized after 60 days.
The difference is greatest at 120 days, after which the difference has converged. In addition, the hematocrit value indicates the amount of red blood cells in the blood, and is related to the activity and oxygen consumption of the fish as well as the number of red blood cells. The hematocrit value of a fish with higher activity is higher, and the total volume of red blood cells in the blood is higher. large. The improvement tendency of the hemoglobin concentration was not constant for two months after administration, and a clear significant difference was recognized only after 90 days, and the same tendency continued thereafter. In addition, hemoglobin is an oxygen carrier in blood, and the hemoglobin concentration becomes higher in a fish that is actively swimming, and the hypoxia resistance is strong. -The immature erythrocyte appearance rate showed an improving tendency immediately after administration, reached a maximum value in 2 weeks, gradually decreased thereafter, and remained almost constant after 90 days. The immature erythrocyte appearance rate is the most effective indicator of the increase or decrease of the hematopoietic function. When there is no immature erythrocyte, the hematopoietic function is reduced. This means that the cause of the enhancement of the hematopoietic function is a problem. -Plasma calcium showed a tendency to improve 4 weeks after administration, a clear significant difference was observed thereafter, and the same trend continued thereafter. In addition, plasma calcium plays various important physiological mechanisms, and its homeostasis must be well maintained. -Plasma sodium showed a tendency to improve immediately after administration, but the trend continued for 60 days after administration, after which the difference had converged. The same can be said for plasma sodium as for plasma calcium. -There is no particular improvement in plasma proteins. The plasma protein indicates the nutritional state of the fish. -After the administration of the blood property improving agent, the survival time of the carp in the air on the 100th day (time until the stop of the operculum movement) is about 6 hours, and when no blood property improving agent is administered, about 2.
6 hours. By the way, the natural product lasted about 7 hours. In addition, the survival time of carp in the air means the quality of respiratory function, if the oxygen binding capacity of hemoglobin in the blood is high, oxygen can be efficiently transported to tissues even in a low oxygen state, and in the air Can survive longer.

According to the invention of claim 2, there is provided a shell fossil in which various nekton, plankton, algae, seaweed and the like made of calcareous or silicic acid are buried and deposited to form a humus-soluble crystal.
Fish feed characterized by heat treatment in the range of 50 ° C. to 300 ° C. to remove water of crystallization and to activate it to form a heat-treated shell fossil, and the heat-treated shell fossil is exclusively used as a color adjusting agent for appreciation fish. It is an additive.

The fossil shellfish used in the present invention is the same as in the case of the above blood property improving agent, and the method of administration is also the same. The properties of the ornamental fish of the present invention as a color development regulator are as follows. -A significant difference was observed about 4 weeks after administration of the color development regulator for the appreciation fish. One of the significant differences is the distinction between the colors. Second, even colors of the same system become more vivid.・ Color dulling disappears in about 4 weeks.

[0023] The invention of claim 3 provides a powdered heat-treated shellfish fossil exclusively used as a blood property improving agent and / or a heat-treated shellfish fossil exclusively used as a color development regulator for appreciation fish, and this powdered heat-treated shellfish fossil is used as a fish feed. It is a method for using a fish feed additive characterized by adding 0.5 to 5.0% by weight, preferably 2.0 to 4.0% by weight.

The particle size of the powdered heat-treated shell fossil is not limited. As a guide, those having a thickness of 75 μm or less are good. After the heat-treated shell fossil is passed through a mesh sieve with an opening of 2 mm and cooled to a normal temperature with a cooler, the heat-treated shell fossil can be used as it is without being crushed. 0.6 mm, the minimum diameter is 5 μm, and the particle size up to 0.2 mm occupies 70%.) However, for administration to fish, it is better to grind the heat-treated shell fossils in order to make the particle size uniform. The amount added to fish feed is 0.5 to 5.0% by weight, preferably 2.0 to 5.0% by weight.
In the range of 4.0% by weight, the most effective is usually 3.0.
0% by weight. If the added amount is less than 0.5% by weight, the blood property improving function and the color adjusting function of the appreciation fish cannot be exhibited. Also, if it is more than 5.0% by weight, it is not so preferable because it has an effect but does not improve, and conversely, fish bite is reduced.

[0025]

DESCRIPTION OF THE PREFERRED EMBODIMENTS Embodiments of the present invention will be described below in detail. First, investigations and tests were conducted to confirm the various effects of the blood property improving agent containing the above-mentioned heat-treated shell fossil as a main component and the color-developing agent for aquarium fish, and the situation will be described. First, Table 5 shows the particle size distribution of the blood property improving agent containing the heat-treated shell fossil of the present invention as a main component and the color developing agent for aquarium fish, and Table 6 shows the data of the dissolution test of water and 0.6% hydrochloric acid. .

[0026]

[Table 5]

[0027]

[Table 6]

According to Table 6, the heat-treated shell fossil is soluble in water, and is soluble in 0.6% hydrochloric acid three times better than water even if it is bad. Therefore, it is suggested that the digestion and absorption of heat-treated shell fossils in fish are possible.

Next, the effect as a blood property improving agent containing a heat-treated shell fossil as a main component will be confirmed. 1. Adjustment of test fish Using 186 carps from Kasumigaura, Ibaraki Prefecture, an indoor flowing pond (237 cm (L) x 158 cm (W) x 31) with aeration equipment at the aquarium center of the Faculty of Fisheries, Hokkaido University
cm (H) = water quantity 1.16 t, water exchange rate once / 30 hours, water exchange rate 740 ml / min) for 2 weeks to acclimate. Thereafter, the body weight was measured [average body weight 35.5 g (2
0 to 56.5 g)], and 93 rats were divided into an experimental group and a control group. 2. Preparation of experimental feed The feed of the experimental group was mixed with the above-mentioned feed moistened with water using a sprayer at a ratio of 3 g of the blood property improving agent to 100 g of the compound feed for carp growth, and allowed to stand for about 5 hours. Let dry. The feed of the control group is only the compound feed for carp breeding.
Table 7 shows the components of the mixed feed for carp cultivation. The blood property improving agents shown in Table 4 are used. 3. Provision of experimental feed to test fish The total weight of the test fish in the experimental group and the test fish in the control group was measured every month, and the amount corresponding to 3% of the total weight was determined once a day (14 days).
Hour to 15:00), and fed 6 days a week. In addition, the compound feed for carp cultivation to which the blood property improving agent was added was deducted from the blood property improving agent, and the compound feed for carp cultivation was 3% of the actual body weight of the experimental group.
It was corrected so that 4. Measurement of body length and body weight of test fish Body length and body weight are measured once a month for all individuals in the experimental group and the control group. The results are shown in Tables 8A and 8B. 5. Sampling of blood from test fish Blood properties are checked twice a month after the start of the experiment and once a month thereafter, so blood is collected from eight fish in each group. In each case, one carp was alternately taken from each group, and the tail was immediately cut off and blood was collected. 6. Test period The test period is from July 1996 to 1997.
Until January, Heisei (Heisei 9). 7. Since carp in the natural group cannot be followed up, one test was conducted 120 days after the start of the experiment for those whose body length and weight were within 15% of the average values of the experimental group and the control group.

[0030]

[Table 7]

[0031]

[Table 8A]

[0032]

[Table 8B]

Example 1 The red blood cell count was calculated every month by diluting blood collected from eight fishes in the experimental group 200 times with Turk's solution and shaking, and then using a hemocytometer prepared under a microscope and a cover glass. The diluted blood was put in between and the calculation was performed 2 minutes later. Comparative Example 1 The red blood cell count is calculated in the same manner as in Example 1 for the blood collected from eight fish in the control group. Comparative Example 2 The red blood cell count was calculated only once, as in Example 1, for blood collected from eight fishes in the natural group. Table 9 shows the results.

[0034]

[Table 9]

According to Table 9, after 30 days from the start of the experiment, the number of red blood cells in the experimental group became larger than that in the control group, which was not much different from the natural group, and the experimental group became more advantageous in respiratory function. Furthermore, the red blood cells in the experimental group are small and large in number, so the total surface area is large, which is extremely advantageous in respiratory function.

Example 2 The concentration of hemoglobin was measured by the cyanmethemoclobin method using a diluent obtained by dissolving 0.1 g of potassium cyanide and 0.7 g of potassium ferricyanide in 1 liter of distilled water.
Every month, 25 μl of blood from 8 animals in the experimental group was injected,
After stirring, the mixture was allowed to stand at room temperature for 30 minutes or more, and the absorbance of this diluted solution was measured at a wavelength of 540 nm using a spectrophotometer (U-2000, manufactured by Hitachi, Ltd.).
Shape). Comparative Example 3 Hemoclobin concentration was measured in the same manner as in Example 2 for blood collected from eight fishes of the control group. Comparative Example 4 The hemoglobin concentration was measured only once in the same manner as in Example 2 for blood collected from eight fishes in the natural group. Table 1 shows the results.
0 is shown.

[0037]

[Table 10]

According to Table 10, the hemoglobin concentration of the experimental group and the control group showed random values until 90 days after the start of the experiment, and thereafter, the hemoglobin concentration of the experimental group became higher, and the hemoglobin concentration of the natural group became lower than that of the experiment. There is almost no difference from the experimental group 120 days later.

Example 3 The hematocrit value (red blood cell volume ratio) was measured by collecting an appropriate amount (about 80%) of blood from eight fishes of the experimental group into a hematocrit tube every month and centrifuging at 4000 rpm for 30 minutes. Separation was performed by indicating the volume ratio of erythrocyte column to total column in%. Comparative Example 5 The hematocrit value was measured in the same manner as in Example 3 for the blood collected from eight fish in the control group. Comparative Example 6 The hematocrit value was measured only once in the same manner as in Example 3 for blood collected from eight fishes in the natural group. Table 1 shows the results.
It is shown in FIG.

[0040]

[Table 11]

According to Table 11, the hematocrit value of the experimental group increased after 30 days from the start of the experiment, suggesting that the resistance to hypoxic saturation was improved, and the erythrocyte count and hemoclobin concentration were increased after 90 days from the control group. Despite being higher,
The decrease in the hematocrit value of the experimental group after 120 days and the disappearance of the difference on the 180th day indicate that the experimental group described above has a small red blood cell and a large number of red blood cells, so that the total surface area is large and the respiratory function is extremely advantageous. Suggests that The hematocrit value of the natural group is almost the same as that of the experimental group 120 days after the start of the experiment.

Example 4 The measurement of the immature erythrocyte appearance rate is as follows. One drop of blood from eight fishes of the experimental group was collected on a slide glass every month, applied thinly on one side of the cover glass, and air-dried at room temperature.
The cells were fixed with 99.5% methyl alcohol for 2 to 3 minutes, air-dried, and stained with a Giemsa diluent obtained by mixing 1 drop of the Giemsa solution with 1 ml of a phosphate buffer of pH 6.4 for 15 to 30 minutes. After dyeing, it was washed with distilled water and air-dried. The cells having a circular cell and nucleus and exhibiting basophilic cytoplasm are defined as immature erythrocytes, and the immature erythrocyte appearance rate is measured by determining the immature erythrocytes per 1000 erythrocytes. COMPARATIVE EXAMPLE 7 The immature erythrocyte appearance rate was measured in the same manner as in Example 4 for blood collected from eight fishes of the control group. Comparative Example 8 The immature erythrocyte appearance rate was measured once in the same manner as in Example 4 for blood collected from eight fishes in the natural group. Table 12 shows the results.

[0043]

[Table 12]

According to Table 12, the immature erythrocyte appearance rates of the experimental group and the control group were both higher in the experimental group immediately after the start of the experiment, and the difference between the two became maximum on the 15th day. Thereafter, it gradually decreased and the difference between the two narrowed to 9
After day 0, there was almost no difference between the two and the appearance rate of immature erythrocytes of both was constant. This is because the test fish suffered from gill rot disease, which increased their hematopoietic function and subsequently restored their health. Further, the fact that the experimental group has a higher immature erythrocyte appearance rate than the control group indicates that the test fish has a stronger ability to restore health. The natural group did not differ from the above two.

Example 5 Measurement of the amount of protein in plasma
and 2.5 ml of 0.01% Coomas using bovine serum albumin as a standard.
To Sie Brilliant Blue G-250, 50 μl of blood from eight fishes of the experimental group was added every month to form a color, and the absorbance of this solution was measured at a wavelength of 595 nm by a spectrophotometer (Hitachi, U-2000 type). The measurement was performed by COMPARATIVE EXAMPLE 9 Plasma protein levels were measured in the same manner as in Example 5 for blood collected from eight fish in the control group. Comparative Example 10 The plasma protein level was measured once in the same manner as in Example 5 for blood collected from eight fishes in the natural group. Table 13 shows the results.

[0046]

[Table 13]

According to Table 13, there was almost no difference between the experimental group and the control group in the plasma protein amount, and there was no difference between the natural group and the natural group. It is known that the carp plasma protein level shows a seasonal variation that is low in the spring and high in the fall, but also in this example on the 60th day from the start of the experiment (July) (9).
(Mon), and was high from autumn to winter after the 90th day (October), showing the same tendency.

Examples 6 and 7 Plasma calcium and sodium were measured by diluting plasma from eight fishes in the experimental group every month with a lanthanum chloride solution adjusted to 1000 ppm with 0.01N hydrochloric acid. The absorbance of the liquid was measured using a spectrophotometer (U-2000, manufactured by Hitachi, Ltd.).
Shape). A mixed solution for flame analysis (for blood, manufactured by Daiichi Kagaku) was used as a standard solution. Plasma calcium and sodium were measured in the same manner as in Example 6 for blood collected from eight fishes of Comparative Examples 11 and 13 control groups. Comparative Examples 12 and 14 Plasma calcium and sodium were measured once in the same manner as in Example 6 for blood collected from eight fishes in the natural group. Tables 14 and 15 show the results.

[0049]

[Table 14]

[0050]

[Table 15]

According to Table 14, it can be said that plasma calcium shows a higher tendency in the physiological mechanism since the experimental group has been in a higher state since 60 days after the start of the experiment. According to Table 15, plasma sodium was 1 after the start of the experiment.
From the 5th day to the 30th day, the experimental group clearly showed a higher state, and after the 60th day, the difference between the two groups disappeared.

Example 8 The measurement of the survival time of carp in air was carried out 1 day after the start of the experiment.
From the experimental group on day 00, 5 fish were quickly taken out, and
As shown in the figure, a wet newspaper 3 was laid on the bottom 2 of the container 1, and five carps 4 from the experimental group were arranged thereon. This is done by setting the time. The average temperature was 16 ° C and the humidity was 64-68%. Comparative Example 15 Five carps 4a were promptly taken from the control group 100 days after the start of the experiment, and the survival time of the carps in the air was measured in the same manner as in Example 7. Comparative Example 16 The survival time of carp in the air was measured in the same manner as in Example 7 also for the five carp 4b having an average body length of 15.2 cm and collected from the natural group. Table 16 shows the results.

[0053]

[Table 16]

According to Table 16, the average survival time of the experimental group was 6.2 hours (maximum 7.1 hours, minimum 5.5 hours), and the average survival time of the control group was 2.6 hours ( Up to 2.
9 hours, minimum 2.4 hours) and the average survival time of the natural group is 7.2 hours (maximum 8.1 hours, minimum 6.8 hours)
It is. Thus, the best in the experimental group outweighs the worst in the natural group. It can be said that this is a carp made of natural products grown artificially.

Next, the effect of the heat-treated shellfish fossil as a main component as a color-developing agent for appreciation fish will be confirmed. 1. Adjustment of test fish Using 30 Nishiki carps from Aichi prefecture, an indoor running water pond (237 cm (L) x 158 cm (W) x 31 cm) with aeration equipment at the aquarium center of the Faculty of Fisheries, Hokkaido University
(H) = 1.16 t, water exchange rate 1 time / 30 hours, water exchange rate 740 ml / min) for 2 weeks for acclimation. Thereafter, the body weight was measured [average body weight 28.5 g (16.6 to 4
2.2 g)], 15 rats were divided into an experimental group and a control group. 2. Preparation of experimental feed The feed of the experimental group was mixed with the above-mentioned feed moistened with water using a sprayer at a ratio of 3 g of the color adjusting agent for appreciation fish to 100 g of the compound feed for carp breeding, and allowed to stand for about 5 hours to dry. I let it. The feed of the control group is only the compound feed for carp breeding.
In addition, the components of the compound feed for carp cultivation are shown in Table 7 and the components of the color development regulator are shown in Table 4. Provision of experimental feed to test fish The total weight of the test fish in the experimental group and the test fish in the control group was measured every month, and the amount corresponding to 3% of the total weight was determined once a day (14 days).
Hour to 15:00), and fed 6 days a week. In addition, the compound feed for carp cultivation to which the color development regulator was added, subtracting the color development regulator,
Correction was made so that the compound feed for carp cultivation was 3% of the actual body weight of the experimental group. 4. Test period The test period is from July 1996 to 1997.
Until January, Heisei (Heisei 9).

EXAMPLE 9 AND COMPARATIVE EXAMPLE 17 Every 15 months, the experimental group and the control group were picked up at 15 fishes each, placed 5 at a time in a white-bottomed container, and compared with the container containing 5 experimental groups. The containers containing the five tails of the group are lined up in three rows. For the first row of containers, from the brightly colored ones, put the Nishiki Goi of the experimental group and the control group separately in each separately prepared container. Count. From the container in the second row where the borders of the colors are clear, put the Nishiki Goi of the experimental group and the control group separately in each separately prepared container, and determine which group Nishiki Goi contains up to the top six fish, Count by panelists. For the third row of containers,
The Nishiki goi of the experimental group and the control group are separately put in each separately prepared container, and the panel is used to count which group of Nishiki goi is contained in the top six fishes. Table 17 shows the results.

[0057]

[Table 17]

According to Table 17, better results were obtained with the experimental group in each of the experiments.

Although only carp was tested, it can be easily predicted that it can be used for other fish that inhabit the inland water, such as eel, trout, yamame, char, ayu, goldfish, and turtle. In addition, regarding the use of the blood property improving agent and the color adjusting agent for appreciation fish of the present invention, fish that inhabit seawater other than fish that inhabit inland waters, for example, Thailand,
Needless to say, it can be applied to hamachi and flounder.

[0060]

As described in detail above, the fish feed additive of the present invention has the following effects. Claim 1
The invention of the invention is that by continuously administering a blood property improving agent to fish, the blood properties of the fish are improved, the health condition is improved, and the survival time of carp, which can be said to be the culmination of the health condition, in the air, is natural. The result was not much different from fish.
Therefore, it can be said that it is a natural fish raised artificially.

According to the second aspect of the present invention, in addition to the effects described above, the color-regulating agent is continuously administered to the ornamental fish.
The colors are vivid, the boundaries between the colors are clear, and the dullness of the colors is eliminated, and the value as an appreciation fish is improved.

According to the third aspect of the present invention, since the blood property improving agent and the color controlling agent for the ornamental fish are simply added to the fish feed at a predetermined ratio and administered to the fish, the above effects can be obtained very easily. Can be done.

[0063]

[Brief description of the drawings]

FIG. 1 is a plan view of a test apparatus for confirming the effects of the present invention.

FIG. 2 is a sectional view of a test apparatus for confirming the effects of the present invention.

[Explanation of symbols]

 1 Container 2 Bottom 3 Newspaper 4 Carp in experimental group 4a Carp in control group 4b Carp in natural group

Claims (3)

[Claims] 1. A shell fossil, which is formed by burying and depositing various nekton, plankton, algae, seaweed and the like made of calcareous or silicic acid, and having a humus-soluble crystal, at 150 ° C. to 300 ° C.
A feed additive for fish, wherein heat treatment is performed within the range of ゜ C to remove water of crystallization and activated to form a heat-treated shell fossil, and the heat-treated shell fossil is exclusively used as a blood property improving agent. 2. Shellfish fossils, which are humus-soluble crystals formed by burying and accumulating various nekton, plankton, algae, seaweeds and the like made of calcareous material, silicic acid, etc., at 150 ° C. to 300 ° C.
A feed additive for fish, wherein heat treatment is carried out in the range of ゜ C to remove water of crystallization and activated to form a heat-treated shell fossil, and the heat-treated shell fossil is exclusively used as a color adjusting agent for appreciation fish. 3. A heat-treated shellfish fossil exclusively used as a blood property improving agent and / or a heat-treated shellfish fossil exclusively used as an admirable fish coloring control agent is powdered, and the powdered heat-treated shellfish fossil is added to a fish feed for 0.5 to 5 days. 0.0% by weight, preferably 2.0 to 4.0% by weight.