JPH10324856A - Adhesive for test specimen, secimen using the same and preparation thereof - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide an adhesive for a test specimen which can be used for bonding a specimen, especially a portion to be measured in a specimen for determining a specific component in a biological sample, to a support and which has a reduced adverse influence on the specimen. SOLUTION: This adhesive for a specimen consists mainly of an acrylic polymer-based resin containing no polymerization initiators (for example, an isocyanate). The adhesive may optionally be reduced in fluidity by compounding a particulate silica and/or a polymer. A silica and/or a polymer are added in a total amount of preferably 0.1 to 20 pts.wt. relative to 100 pts.wt. of the acrylic polymer-based resin. The acrylic polymer-based resin is a (co)polymer of one or more of acrylic esters or a copolymer thereof with another monomer. Adhesive layer 2 is foamed by applying the above-mentioned adhesive on support 3, and a test specimen is prepared by laminating a portion to be measured 4 on the adhesive layer 2 containing a reagent layer 1.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

TECHNICAL FIELD The present invention relates to a test piece adhesive,
The present invention relates to a test piece using the same as an adhesive layer, particularly a test piece for measuring a specific component in a biological sample, and a method for producing the same.

[0002]

2. Description of the Related Art In general, a test piece for measuring a specific component is formed by adhering a measuring portion including a reagent layer containing a reagent to a support, and bonding the measuring portion to the support. The method includes the following. 1) Adhere via a double-sided adhesive tape. 2) Adhesion is performed using an adhesive used for a double-sided adhesive tape or the like. However, when a conventional adhesive, including a double-sided tape, is used, trace components in the adhesive may cause coloration of the measurement section and deterioration of the reagent. Polymerization initiator (peroxide, metal, etc.)
It is thought to be caused by such things. This coloring and alteration occurs at an early stage and over time,
In particular, in the case of a test piece for measuring a specific component in urine, the measurement is often performed using a coloring reaction, so that the influence of coloring of the test piece is large and causes an error factor.

[0003]

DISCLOSURE OF THE INVENTION The present invention relates to a test strip, particularly a test strip for measuring a specific component in a biological sample, which has a small effect on a reagent for bonding a measuring portion of the test strip to a support. The purpose of the present invention is to provide a pressure-sensitive adhesive.

[0004]

The present invention mainly comprises a pressure-sensitive adhesive for a test piece, which comprises an acrylic polymer-based resin containing no polymerization initiator, and at least one layer including a reagent layer. A test piece characterized in that the measurement part and the support are fixed via an adhesive layer made of the test piece adhesive, especially a test piece for measuring a specific component in a biological sample, And a method for producing them.

[0005]

DESCRIPTION OF THE PREFERRED EMBODIMENTS The acrylic polymer resin used in the present invention does not contain a polymerization initiator such as a peroxide or a metal,
That is, the polymerization initiator is not used at the time of polymerization, and has a sufficient adhesive force as an adhesive for the test piece when bonding the measurement portion of the test piece to the support, and does not adversely affect the reagent. It is not particularly limited as long as it is one.

In the present invention, the acrylic polymer-based resin is mainly a polymer composed of one or more acrylate monomers (including a copolymer composed of two or more acrylate monomers) or one acrylate monomer. It is a copolymer of the above-mentioned acrylate monomer and a component (vinyl acetate, methacrylic ester, hydroxyl group monomer, unsaturated carboxylic acid monomer, etc.) which gives adhesion or cohesion.

The acrylate constituting the acrylic polymer resin used in the present invention may be appropriately selected depending on the required adhesiveness and the like, and is not particularly limited.
Examples include methyl acrylate, ethyl acrylate, propyl acrylate, butyl acrylate, 2-ethylhexyl acrylate, and the like.

Examples of the monomer copolymerized with the acrylate ester include vinyl acetate, styrene, hydroxyl monomers such as hydroxyethyl acrylate and hydroxypropyl methacrylate, and unsaturated carboxylic acids such as methacrylic acid and acrylic acid. .

The acrylic polymer resin used in the present invention is:
The above monomers are generally polymerized or copolymerized using a known method, but no polymerization initiator is used.

The pressure-sensitive adhesive for test pieces of the present invention may be a solvent-type pressure-sensitive adhesive obtained by dissolving an acrylic polymer-based resin in a solvent, or a solvent-free pressure-sensitive adhesive obtained by emulsifying an acrylic polymer-based resin by emulsion polymerization or the like. It may be.

In the case of a solvent-type pressure-sensitive adhesive, the solvent used is not particularly limited as long as it does not interfere with the operation of the present invention, and examples thereof include ethyl acetate, toluene, and methyl ethyl ketone. As the method of dissolving in the solvent, the one used as a solvent at the time of polymerization of the acrylic polymer resin may be left, or the acrylic polymer resin may be mixed and dissolved in the solvent by an ordinary method.

In general, a pressure-sensitive adhesive using an acrylic polymer-based resin increases the molecular weight of the acrylic polymer-based resin by crosslinking by using a polymerization initiator such as isocyanate at the time of polymerization of the acrylic polymer-based resin. Therefore, since the acrylic polymer resin used in the present invention does not contain a polymerization initiator, the molecular weight of the acrylic polymer resin is small, the fluidity is high (no stiffness), and the performance as an adhesive is reduced. . However, in the case of a test piece, the measuring part has a small area, is lightweight, and is usually used in such a manner that a test liquid is dropped on the measuring part or the measuring part is immersed in the test liquid. A large adhesive strength is not required unless it is peeled off by dissolution with a liquid. Rather, at the time of manufacture
The components in the adhesive react with the reagent components contained during storage,
Deterioration and coloring are more important. Therefore, by using a pressure-sensitive adhesive that does not contain a polymerization initiator having the highest reactivity as a component contained in the resin (particularly, isocyanate, etc.) for adhesion between the measurement portion of the test piece and the support, Deterioration and coloring of reagent components can be prevented.

When the thickness of the pressure-sensitive adhesive layer is increased, the pressure-sensitive adhesive for a test piece made of an acrylic polymer-based resin containing no polymerization initiator as described above creeps over time due to its fluidity. It easily spreads out of the application range. In this case, the test pieces adhere to each other during storage, making it difficult to remove the test pieces from the storage container during use, or sticking to a measuring device or the like during measurement, or causing the test pieces to adhere to each other in the measuring device and cause clogging. Problem arises.

Therefore, the pressure-sensitive adhesive for a test piece of the present invention may contain finely divided silica and / or a polymer if necessary.
The fluidity may be reduced.

The fine particle silica has a particle diameter of about 7 to 40 μm.
It is desirable to use a uniform one. As such fine particle silica, for example, silica (Nippon Aerosil Co., Ltd.)
Manufactured by R972).

The polymer may be a hydrophobic polymer or a hydrophilic polymer, and is not particularly limited as long as it does not interfere with the operation of the present invention and can be uniformly mixed with the acrylic polymer resin. . As such a polymer, for example, polyvinyl butyral (Sekisui Chemical Co., Ltd.)
Manufactured by Esrec BM-2), hydroxypropylcellulose (manufactured by Nippon Soda Co., Ltd.) and the like.

In the present invention, the amount of the fine particle silica and / or the polymer is preferably 0.1 to 20 parts by weight, more preferably 1 to 20 parts by weight, based on 100 parts by weight of the acrylic polymer resin. It is better to mix 5 parts by weight.

In the present invention, the method of blending the particulate silica and / or the polymer into the pressure-sensitive adhesive may be a commonly known method, and the particle silica and / or the polymer is added to the pressure-sensitive adhesive for a test piece composed of an acrylic polymer resin. A method of adding, mixing with a stirrer or the like is used.

The pressure-sensitive adhesive for test pieces of the present invention may contain, in addition to the finely divided silica and / or the polymer, other components which do not interfere with the operation of the present invention.

The above-mentioned pressure-sensitive adhesive on a support is mainly composed of the pressure-sensitive adhesive for a test piece mainly composed of an acrylic polymer resin, or the pressure-sensitive adhesive for a test piece obtained by mixing fine particle silica and / or polymer with the pressure-sensitive adhesive for the test piece. By forming a layer and fixing the measuring section including the reagent layer via the pressure-sensitive adhesive layer, it is possible to obtain a test piece in which deterioration and coloring of the reagent component in the measuring section are prevented.

[0021] In the present invention, the test piece is a test piece in which a measuring part comprising at least one layer including a reagent layer is fixed to a support, and the adhesive layer between the support and the measuring part is provided. The configuration and manufacturing method other than using the pressure-sensitive adhesive for a test piece of the present invention are not particularly limited, and the same configuration and manufacturing method as those generally used can be used.

In the test piece of the present invention, the method for forming the pressure-sensitive adhesive layer using the pressure-sensitive adhesive for a test piece can be a usual method such as coating, and the pressure-sensitive adhesive layer has the required adhesive strength and pressure-sensitive adhesive. It may be appropriately formed in a planar shape, a linear shape, an island shape, or the like depending on the amount or the like. When a solvent-type pressure-sensitive adhesive is used, the solvent may be removed by a generally known drying method.

Hereinafter, the present invention will be described in more detail with reference to Test Examples and Examples, but the present invention is not limited thereto. Test Example The test pieces of Examples 1 to 3 and Comparative Example 1 were placed in a brown bottle containing a desiccant and stored at 40 ° C. for 2 weeks, and the color change and the presence or absence of the adhesive sticking out of the measuring section were visually determined. did. Table 1 shows the test results. * Presence or absence of adhesive sticking out of measuring part ○: None △: Slightly sticking out

[0024]

[Table 1] From Table 1, it can be seen that the test piece of the present invention does not show coloring due to the reaction between the pressure-sensitive adhesive and the contained reagent over time, and the addition of silica or a polymer improves the creep of the pressure-sensitive adhesive. I understand.

[0025]

EXAMPLES Example 1 The first stage impregnation liquid formulation was as follows: cumene hydroperoxide (manufactured by Nacalai Tesque, Inc.) 3 ml ethyl alcohol (manufactured by Nacalai Tesque, Inc.) 30 ml citric acid (manufactured by Nakarai Tesque, Inc.) 1.7 g Trisodium citrate (manufactured by Nacalai Tesque, Ltd.) 9.6 g Purified water 100 ml As a second stage impregnation liquid formulation, 2-aminobenzothiazole (manufactured by Tokyo Chemical Industry Co., Ltd.) 1 g 3, 3 ', 5,5',-tetramethylbenzidine 500 mg Toluene (manufactured by Nacalai Tesque, Inc.) 70 ml Ethyl alcohol (manufactured by Nacalai Tesque, Inc.) 30 ml Filter paper cut into 30 cm × 30 cm (Whatman; 3
MMchr) was impregnated with the first-stage impregnating solution, pulled up, blow-dried at 80 ° C. for 30 minutes, then impregnated with the second-stage impregnating solution, pulled up, and blow-dried at 50 ° C. for 10 minutes.
This was cut into a size of 5 mm x 5 mm, which was used as a measuring portion (reagent layer) of the test paper for occult blood measurement. Saibinol AT-D40 (manufactured by Seiden Chemical Co., Ltd.) was used as an adhesive made of an acrylic polymer-based resin. Coating thickness: 75
μm), the above-mentioned measuring part was adhered to obtain a test piece.

Example 2 2.5 g of silica (R972 manufactured by Nippon Aerosil Co., Ltd.) was mixed with 100 g of the pressure-sensitive adhesive used in Example 1 and mixed with stirring to obtain a pressure-sensitive adhesive. This adhesive is used as a support for 5 mm ×
After applying to a tip of a 60 mm white polyethylene terephthalate film in a size of 5 mm × 5 mm (coating thickness: 75 μm),
The same measurement part as in Example 1 was adhered to obtain a test piece.

Example 3 2.5 g of hydroxypropylcellulose (manufactured by Nippon Soda Co., Ltd.) was mixed with 100 g of the adhesive used in Example 1 and mixed with stirring to obtain an adhesive. This adhesive is used as the support 5
5 mm x 5 mm applied to the end of a white polyethylene terephthalate film of 50 mm x 60 mm (coating thickness: 75μ)
m) Thereafter, the same measurement section as in Example 1 was adhered to obtain a test piece.

Comparative Example 1 A measuring section similar to that of Example 1 was mounted on a support, 5 mm × 60 mm.
A conventional double-sided adhesive tape (acrylic polymer-based pressure-sensitive adhesive containing isocyanate) was adhered to the tip of a white polyethylene terephthalate film having a thickness of 1 mm to obtain a test piece.

[0029]

Since the pressure-sensitive adhesive for test pieces of the present invention does not contain a highly reactive polymerization initiator (especially, isocyanate, etc.), the pressure-sensitive adhesive for test pieces of the present invention is used for adhesion between the measurement portion of the test piece and the support. The use of the adhesive makes it possible to prevent deterioration and coloring of the reagent components in the measuring section during production and storage of the test piece. In addition, by blending the fine particle silica and / or polymer, the fluidity is lowered to reduce creep over time, and adhesion between test pieces during storage / measurement or adhesion with equipment during measurement is prevented. You.

[Brief description of the drawings]

FIG. 1 is a diagram illustrating a configuration of a test piece of the present invention.

[Explanation of symbols]

 DESCRIPTION OF SYMBOLS 1 Reagent layer 2 Adhesive layer 3 Support 4 Measurement part

──────────────────────────────────────────────────続 き Continued on the front page (51) Int.Cl. ⁶ Identification code FI G01N 33/543 591 G01N 33/543 591

Claims (6)

[Claims] A pressure-sensitive adhesive for a test piece, comprising mainly an acrylic polymer-based resin containing no polymerization initiator. 2. The test piece pressure-sensitive adhesive according to claim 1, wherein fine particle silica and / or a polymer are blended. 3. A method according to claim 1, wherein the fine particle silica and / or the polymer is added in an amount of 0.1 parts by weight based on 100 parts by weight of the acrylic polymer resin.
The pressure-sensitive adhesive for a test piece according to claim 2, wherein the adhesive is used in an amount of 20 to 20 parts by weight. 4. A test piece comprising a measuring part comprising at least one layer including a reagent layer fixed to a support,
A test piece, wherein the measuring part and the support are fixed via an adhesive layer made of the pressure-sensitive adhesive for a test piece according to any one of claims 1 to 3. 5. The test strip according to claim 4, which is a test strip for measuring a specific component in a biological sample. 6. A pressure-sensitive adhesive layer is formed by applying the pressure-sensitive adhesive for a test piece according to claim 1 on a support, and at least a reagent layer is provided on the pressure-sensitive adhesive layer. A method for producing a test piece, comprising: laminating a measuring part comprising at least one layer.