JPH10319020A - Analyzing device - Google Patents

Abstract

PROBLEM TO BE SOLVED: To prevent a pressurized reagent solution from flowing back in a reagent stirring gas piping by constituting the opening and closing valve-side area between an opening valve and the reagent solution by use of a piping of highly airtight material. SOLUTION: A ninhydrin reagent vessel 1 is internally pressurized to a pressure higher than the atmospheric pressure by nitrogen gas, and this pressure is also transferred into a reagent stirring gas piping 35 through a ninhydrin reagent solution 36. The piping 36 is filled with nitrogen gas, and this nitrogen gas is permeated through the circumferential wall of a permeable Teflon tube 35c to reduce the volume. A reagent solution 11 thus advances (flows back) to the tube 35c. Since the nitrogen gas is left within a highly airtight stainless pipe 35a when it flows back to the position of a relay joint 35b, the nitrogen gas is never leaked or reduced. Thus, the back flow of the reagent solution 36 is stopped in this position, and the reagent solution 36 can be prevented from flowing back to a solenoid valve 31.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method for injecting an inert gas into a reagent solution to perform stirring for preparing the reagent solution, and then blowing an inert gas into the reagent container to remove the reagent solution in the reagent container. The present invention relates to an analyzer such as an amino acid analyzer for applying pressure.

[0002]

2. Description of the Related Art In order to prepare a ninhydrin reagent, an amino acid analyzer is used for bubbling by blowing nitrogen gas through a gas supply pipe for reagent agitation in which the tip is buried and opened in a reagent solution in a reagent container. There is a stirring function to perform.
This stirring means controls the supply of nitrogen gas by providing an electromagnetic valve or cock as a control opening / closing valve in the middle of the reagent stirring gas supply pipe connecting the nitrogen gas supply source and the reagent solution. The control on-off valve is closed to stop the supply. Then, a nitrogen gas for pressurizing the reagent solution is supplied into the reagent container. Generally, a Teflon (registered trademark) tube is often used for these nitrogen gas supply pipes from the viewpoint of operability and chemical resistance.

[0003]

However, in such an analyzer, when the reagent solution is maintained in a pressurized state for a long time, the pressurized reagent solution is supplied with a Teflon-made reagent stirring gas supply. There is a problem that the pipe flows backward to reach the control on-off valve, and the control on-off valve is corroded and broken.

An object of the present invention is to prevent a pressurized reagent solution from excessively flowing back through a reagent stirring gas supply pipe.

Another object of the present invention is to prevent a pressurized reagent solution from flowing back through a gas supply pipe for stirring a sample and adversely affecting a control opening / closing valve provided in the gas supply pipe for stirring. Is to do.

It is still another object of the present invention to prevent a pressurized reagent solution from flowing back through a sample stirring gas supply pipe.

It is still another object of the present invention to prevent the above-mentioned reverse flow of the reagent solution with a simple structure.

[0008]

The inventor of the present invention has found that the reason why the pressurized reagent liquid flows back through the reagent stirring gas supply pipe is that the gas in the reagent stirring gas supply pipe is caused by the reagent stirring gas supply pipe. The gas pressure in the reagent stirring gas supply pipe is reduced by passing through the peripheral wall and passing out of the pipe due to the air permeability,
It has been discovered that the pressurized reagent solution is to be pushed up.

Accordingly, the present invention is to prevent the gas from leaking out of the reagent stirring gas supply pipe or to replenish the leaked gas, thereby preventing the gas pressure in the reagent stirring gas supply pipe from lowering. This prevents the pressurized reagent liquid from flowing back through the reagent stirring gas supply pipe.

One feature of the present invention is an analyzer provided with an on-off valve for gas supply control in the middle of a reagent stirring gas supply pipe connecting a gas supply source and a reagent solution. The area between the open / close valve side of the pipe between the pipes is made of a pipe material of a highly airtight material, so that when the reagent liquid is pressurized in a state where the open / close valve is closed, the gas in the pipe becomes the pipe. To prevent the reagent solution from flowing through and passing through the peripheral wall to prevent the reagent solution from flowing backward.

The highly airtight piping material only needs to be able to prevent the gas in the piping, which is pressurized by the reagent solution, from passing through the peripheral wall of the piping and dropping out, thereby preventing the reagent solution from being pushed up and flowing back to the on-off valve. Therefore, it may be provided in the area on the opening / closing valve side. The region in contact with the reagent solution and the region in the vicinity thereof are preferably made of a Teflon tube having high chemical resistance.

Another feature of the present invention is that a sealable reagent container containing a reagent solution, a reagent stirring gas supply pipe for supplying a stirring gas into the reagent solution contained in the reagent container, A gas supply pipe for controlling the supply of reagent for stirring and a gas supply pipe for supplying gas for reagent pressurization to the space inside the reagent container, which is provided in the middle of the gas supply pipe for reagent supply, and the reagent container is sealed. In the analyzer, a reagent pressurizing gas is supplied into the reagent container to pressurize the reagent liquid in the reagent container, a reagent stirring gas connecting between the reagent container and the reagent stirring gas supply control opening / closing valve. The region of the gas supply pipe on the connection side with the on-off valve for controlling the supply of the reagent for stirring the gas is constituted by a tube material of a material that is highly airtight with respect to the gas for stirring the reagent, thereby preventing gas from leaking out. Prevent liquid backflow Lies in the fact.

Still another feature of the present invention is that a sealable reagent container containing a reagent solution, a pressurized gas generating source for generating a pressurized gas, and a pressurized gas supply pipe from the pressurized gas generating source are provided. A gas supply pipe for stirring the reagent that supplies the pressurized gas supplied through the reagent container contained in the reagent container, and a pressurized gas supplied through the pressurized gas supply pipe inside the reagent container. Equipped with a gas supply pipe for reagent pressurization to be supplied to the space,
With the reagent container open, a pressurized gas is blown into the reagent liquid by the reagent stirring gas supply pipe to perform stirring, and the reagent container is sealed by the reagent reagent pressurization gas supply pipe while the reagent container is sealed. In an analyzer configured to supply a pressurized gas to pressurize the reagent liquid in the reagent container, a reagent pressurized gas supply control may be provided in the middle of the pressurized gas supply pipe and the middle of the reagent pressurized gas supply pipe. The on-off valve is provided. According to such an analyzer, the pressurized gas supplied to the reagent container to pressurize the reagent solution is also supplied to the reagent stirring gas supply pipe. Even if there is a gas bleeding phenomenon due to the gas permeability from the peripheral wall of the pipe, the pressure in the pipe does not decrease and the reagent liquid does not flow backward.

Still another feature of the present invention is that a reagent solution is accommodated in a sealable reagent container, and a reagent stirring gas is blown into the reagent solution through a reagent stirring gas supply pipe with the reagent container opened. In an analyzer in which a reagent solution is stirred and a reagent pressurizing gas is supplied into the reagent container in a state where the reagent container is sealed to pressurize the reagent solution, the reagent pressurizing gas is supplied to the reagent stirring gas. Another object of the present invention is to provide a gas replenishing means for supplying the gas to the pipe, so as to replenish the gas leaked due to the gas permeability of the gas supply pipe for reagent agitation to prevent the pressure in the pipe from being reduced.

[0015]

Embodiments of the present invention will be described below.

FIG. 1 is a system configuration diagram of an amino acid analyzer. Reference numerals 1 to 4 denote first to fourth buffer containers for storing the first to fourth buffers, respectively, and reference numeral 5 denotes a column regenerating solution container for storing the column regenerating solution. The solenoid valve series 6 selects one of these containers 1 to 5 and communicates it with the buffer pump 7, and the buffer or regenerating solution in the selected container is supplied by the buffer pump 7 to the ammonia filter column 8, the autosampler It is sent to the separation column 10 via 9.

The amino acid sample introduced by the autosampler 9 is separated by a separation column 10. The separated amino acids are mixed with the ninhydrin reagent and ninhydrin buffer supplied from the ninhydrin reagent container 11 and the ninhydrin buffer container 17 by the ninhydrin pump 12 in the mixer 13 and reacted in the reaction tube 14. The amino acids that are colored by the reaction are continuously detected by the detector 15, output as a chromatogram and data as shown in FIG. 4 by the data processor 16, and recorded and stored. The detector 15 includes a flow cell 18 and a lamp 19.

The nitrogen gas supply system is configured to supply a pressure gas (nitrogen gas) from a nitrogen gas cylinder 20 as a nitrogen gas generation source to the ninhydrin reagent container 11 and the ninhydrin buffer solution container 17. The purpose of the nitrogen gas supply system is to assist the supply of the ninhydrin reagent solution by pressurizing the ninhydrin reagent container 11, to prepare a ninhydrin reagent that mixes a plurality of solutions by blowing nitrogen gas into the reagent solution and bubbling. Filling of the gas region in the reagent container with inert nitrogen gas to prevent oxidation of the ninhydrin reagent.

FIG. 2 shows a basic piping configuration and an operation mode of an embodiment of a nitrogen gas supply system for supplying a nitrogen gas to the ninhydrin reagent container 11.

The nitrogen gas supply system includes a nitrogen gas cylinder 2
High pressure nitrogen gas generated at 0
1, the pressure is adjusted to a predetermined gas pressure and then branched, and one of the branches is connected so as to be supplied to a two-way solenoid valve 31 which is a gas supply control opening / closing valve for reagent stirring. The other is connected to be supplied to a three-way solenoid valve 32 which is an on-off valve for controlling the supply of pressurized gas. The tip of the two-way solenoid valve 31 is connected to the ninhydrin reagent container 11.
It is submerged in a ninhydrin reagent solution 36 therein and is connected to a reagent stirring gas supply pipe 35 provided to open in the solution. The three-way electromagnetic valve 32 is connected to a reagent pressurizing gas supply pipe 33 provided to open in a space in the ninhydrin reagent container 11 and an exhaust tube 34 opened to the atmosphere.

Further, from the ninhydrin reagent container 11,
A reagent supply pipe 23 for supplying the pressurized ninhydrin reagent liquid 36 to the ninhydrin pump 12 is led out.

The operation mode of the nitrogen gas supply system is as follows.
There are three things:

(1) At the time of preparing ninhydrin As shown in FIG. 2A, the two-way solenoid valve 31 is opened, and the three-way solenoid valve 32 operates to connect the reagent pressurizing gas supply pipe 33 to the exhaust tube 34. Then, the inside of the ninhydrin reagent container 11 is opened to the atmosphere. In this state, when the pressurized nitrogen gas generated in the nitrogen gas cylinder 20 is supplied, the pressurized nitrogen gas is supplied from the tip opening of the reagent stirring gas supply pipe 35 submerged in the ninhydrin reagent solution 36. The ninhydrin reagent solution 36 is bubbled into the mixture (bubble with nitrogen gas).

(2) At the time of analysis As shown in FIG. 2 (2), the two-way solenoid valve 31 is closed, and the three-way solenoid valve 32 connects the pressurized gas supply pipe 21 with the reagent pressurized gas supply pipe 33. To work. At this time, the inside of the ninhydrin reagent container 11 is filled with a nitrogen gas cylinder 2.
0.03 above atmospheric pressure by supplying pressurized nitrogen gas from 0
It is pressurized to a pressure higher by 4 to 0.04 MPa.

According to the present invention, the nitrogen gas for stirring remaining in the gas supply pipe 35 for stirring reagent in such a pressurized state at the time of analysis escapes from the peripheral wall due to the gas permeability of the gas supply pipe 35 for stirring reagent. This prevents the ninhydrin reagent solution 36 from excessively flowing back into the reagent stirring gas supply pipe 35.

(3) When Replacing Reagents As shown in FIG. 2C, the two-way solenoid valve 31 is closed so that the three-way solenoid valve 32 connects the reagent pressurizing gas supply pipe 33 and the exhaust tube 34. Make it work. This allows
The supply of the pressurized nitrogen gas into the ninhydrin reagent container 11 is stopped, and the ninhydrin reagent container 11 is further opened to the atmosphere, so that the ninhydrin reagent solution 36 can be exchanged.

Next, a specific example of the piping of the nitrogen gas supply system will be described with reference to FIG.

A nitrogen gas cylinder 20 as a nitrogen gas generating source
Is connected to the input side of the pressure reducing valve 22 in the amino acid analyzer by a resin tube 21 which is a pressurized gas supply pipe. The pressure reducing valve 22 reduces the pressure of the nitrogen gas supplied from the nitrogen gas cylinder 20 to a pressure higher than the atmospheric pressure by 0.04 MPa. The pipe on the output side of the pressure reducing valve 22 is branched, one of which is connected to the input side of the two-way solenoid valve 31 and the other is connected to the input side of the three-way solenoid valve 32.

The gas supply pipe 35 for stirring reagent connected to the output side of the two-way solenoid valve 31 has a connection side with the two-way solenoid valve 31 having an arbitrary thickness and length, for example, having an inner diameter of 0.8 mm and a length of 0.8 mm. Is a 100 mm stainless steel pipe 35a, and the tip of a tetrafluoroethylene tube (Teflon tube) 35c connected to the end of the stainless steel pipe 35a via a relay joint 35b is immersed in the ninhydrin reagent solution 11 and opened. Configure. Since the airtightness of the connection between the two-way solenoid valve 31 and the stainless steel pipe 35a is important, it is desirable to connect the stainless steel pipe 35a to a position as close as possible to the valve body sealing part of the two-way solenoid valve 31.

Each of the reagent pressurizing gas supply pipe 33 and the exhaust tube 34 connected to the three-way solenoid valve 32 is a Teflon tube.

The nitrogen gas supply system configured as above is
In the state at the time of analysis, the inside of the ninhydrin reagent container 11 is set at 0.034 to 0.04
The pressure is increased to a pressure higher by MPa, and this pressure is also transmitted to the reagent stirring gas supply pipe 35 via the ninhydrin reagent solution 36. The nitrogen gas supplied at the time of preparation is filled in the reagent stirring gas supply pipe 35, and this nitrogen gas permeates the peripheral wall of the Teflon tube 35c by being pressurized through the ninhydrin reagent solution 36. And escapes into the atmosphere, reducing its volume. This is because the Teflon tube 35c has a slight gas-permeating property (air permeability). Then, as the nitrogen gas in the Teflon tube 35c decreases, the ninhydrin reagent solution 11 enters the Teflon tube 35 (backflow).
However, the amount increases with time.

However, when the gas flows backward to the position of the relay joint 35b, the nitrogen gas therebelow remains in the highly airtight (non-breathable) stainless steel pipe 35a. Therefore, the backflow of the ninhydrin reagent solution 36 also stops at this position, and the backflow of the ninhydrin reagent solution 36 to the two-way solenoid valve 31 can be prevented.

In this embodiment, a stainless steel pipe was used as a highly airtight piping material, and a Teflon tube having low airtightness but excellent in operability and chemical resistance was used as other piping materials. Similar effects can be obtained by using a pipe member of the material shown. In Table 1, PE
EK is a polyetheretherketone.

[0034]

[Table 1]

FIG. 5 shows a piping configuration and an operation mode of another embodiment of the nitrogen gas supply system in the analyzer according to the present invention. In this embodiment, even if the nitrogen gas in the reagent stirring gas supply pipe 35 passes through the peripheral wall of the reagent stirring gas supply pipe 35 and exits, the reagent stirring gas supply pipe 35
By preventing the pressure of the nitrogen gas inside from decreasing, the backflow of the pressurized reagent solution 36 is prevented. FIG.
The same reference numerals are given to the components equivalent to those of the embodiment shown in FIG.

A pipe on the output side of the pressure reducing valve 22 is connected to a three-way solenoid valve 32 via a two-way solenoid valve 37 which is a main opening / closing valve for gas supply control. The tube 38 is branched and its tip is provided so as to open into the ninhydrin reagent solution 36 in the ninhydrin reagent container 11.

When the ninhydrin is prepared, the nitrogen gas supply system configured as described above operates the three-way solenoid valve 32 such that the reagent pressurizing gas supply pipe 33 communicates with the exhaust tube 34 as shown in FIG. By operating and opening the two-way solenoid valve 37, the nitrogen gas regulated by the pressure reducing valve 22 is supplied to the ninhydrin reagent container 1 through the Teflon tube 38.
Bubbling can be performed by blowing into the ninhydrin reagent solution 36 in 1.

At the time of analysis, the three-way solenoid valve 32 is operated so that the nitrogen gas output from the two-way solenoid valve 37 is supplied to the reagent pressurizing gas supply pipe 33 as shown in FIG. Thus, the ninhydrin reagent 36 can be pressurized by pressurizing the inside of the ninhydrin reagent container 11. In this state, the nitrogen gas in the Teflon tube 38 serving as the reagent stirring gas supply pipe escapes due to the air permeability of the peripheral wall of the Teflon tube 38, and the escaped nitrogen gas is supplied from the two-way solenoid valve 37. Since the inside of the Teflon tube 38 communicates with the inside of the ninhydrin reagent container 11 via the three-way solenoid valve 32 and the reagent pressurizing gas supply pipe 33 and is in the same pressure state, the pressurized ninhydrin in the ninhydrin reagent container 11 is Reagent 3
6 does not flow backward in the Teflon tube 38.

At the time of replacing the reagent, FIG.
As shown in (2), the supply of nitrogen gas is stopped by closing the two-way solenoid valve 37, and the gas supply pipe 33 for reagent pressurization is connected to the exhaust tube 34 to open the ninvidrin reagent container 11 to the atmosphere. When the solenoid valve 32 is operated, the inside of the ninhydrin reagent container 11 is brought into the atmospheric pressure state, and the ninhydrin reagent solution 36 can be replaced.

In this embodiment, the Teflon tube 38, which is a gas supply pipe for stirring the reagent, is not shut off by the on-off valve to form a private chamber. The pressure in the Teflon tube 38 does not decrease because the pressure is supplied from the solenoid valve 37. Further, since the inside of the Teflon tube 38 and the inside of the ninhydrin reagent container 11 for pressurizing the ninhydrin reagent solution 36 communicate with each other, a pressure difference that causes the ninhydrin reagent solution 36 to flow back into the Teflon tube 38 does not occur. Therefore, in this embodiment, the gas supply pipe for reagent stirring (Teflon tube 3
In the case of 8), it is not necessary to consider the backflow of the ninhydrin reagent solution 36 due to gas release due to the gas permeability of the peripheral wall.

As described above, in the embodiment in which the nitrogen gas is supplied to the reagent stirring gas supply pipe even when the reagent solution is pressurized, the opening / closing timing of the two-way solenoid valve 31 is changed in the nitrogen gas supply system shown in FIG. Can also be realized. The nitrogen gas supply system shown in FIG. 2 was operated to close the two-way solenoid valve 31 during analysis (reagent pressurization).
If the control program is changed so that the two-way solenoid valve 31 is kept open during the analysis, nitrogen gas can be supplied to the reagent stirring gas supply pipe 35 even during the analysis. The gas supply pipe 35 can use a Teflon tube in the whole area. Such a modification is simple because the solenoid valve control program by the control device (not shown) for controlling the two-way solenoid valve 31 may be changed.

In the embodiment in which nitrogen gas is supplied to the reagent stirring gas supply pipe even when the reagent solution is pressurized, the reagent stirring gas supply pipe 35 in the nitrogen gas supply system shown in FIG.
It can also be realized by connecting the reagent supply gas supply pipe 33 with a pipe (not shown) interposed with a two-way solenoid valve for gas supply control (not shown). In such a modification, the gas supply control two-way solenoid valve is closed when preparing ninhydrin, and the reagent supply gas supply pipe 35
The nitrogen gas supplied to the reagent liquid is effectively blown into the reagent solution, and is opened during analysis (reagent pressurization) to supply the nitrogen gas flowing out of the reagent stirring gas supply pipe 35 from the reagent pressurization gas supply pipe 33. Thus, the gas pressure in the reagent stirring gas supply pipe 35 is prevented from lowering, and is kept open when the reagent is replaced. Such opening / closing control of the gas supply control two-way solenoid valve can be realized by changing the solenoid valve control program in the control device.

In the embodiments described above, the reagent liquid 36 in the ninhydrin reagent container 11 is prepared and pressurized using nitrogen gas. However, an inert gas such as helium gas or argon gas is used. The same effect can be obtained even with the configuration.

[0044]

According to one aspect of the present invention, the reagent agitation gas supply pipe for blowing the reagent agitation gas into the reagent has a highly airtight tube material in the connection area with the on-off valve. It is possible to prevent the stirring gas from leaking from the supply pipe and prevent the pressurized reagent from flowing backward through the reagent stirring gas supply pipe and entering the on-off valve.

According to another aspect of the present invention, the pressurized gas is supplied to the reagent stirring gas supply pipe even when the reagent is pressurized. Therefore, when the gas escapes from the reagent stirring gas supply pipe, the reagent stirring gas supply The pressure in the pipe does not decrease, and therefore, the pressurized reagent does not flow back through the reagent stirring gas supply pipe.

Further, since the safety of the gas supply system is improved, the safety of the analyzer is improved.

Further, these inventions can be realized with a simple configuration in which only a part of the piping material is changed, an on-off valve is added, the installation position is changed, or the on-off control timing of the on-off valve is changed.

[Brief description of the drawings]

FIG. 1 is a system configuration diagram of an amino acid analyzer according to the present invention.

FIG. 2 is an explanatory view of a piping form and an operation mode showing one embodiment of a nitrogen gas supply system in the amino acid analyzer according to the present invention shown in FIG.

FIG. 3 is an explanatory diagram of a piping configuration of a nitrogen gas supply system and a reagent backflow state in the amino acid analyzer according to the present invention shown in FIG. 1;

FIG. 4 is a chromatogram of amino acids.

FIG. 5 is a diagram illustrating a piping configuration and an operation mode of another embodiment of the nitrogen gas supply system in the amino acid analyzer according to the present invention.

[Explanation of symbols]

11: Ninhydrin reagent container, 20: Nitrogen gas cylinder,
21 ... resin tube, 23 ... reagent supply pipe, 31 ... 2
One-way solenoid valve, 32 ... three-way solenoid valve, 33 ... reagent pressurizing gas supply pipe, 35 ... reagent stirring gas supply pipe, 35a ... stainless steel pipe, 35b ... relay joint, 35c ... Teflon tube, 36 ... ninhydrin reagent liquid.

 ──────────────────────────────────────────────────続 き Continuing from the front page (72) Inventor Hiroshi Satake 882 Omo, Oaza-shi, Hitachinaka-shi, Ibaraki Pref.

Claims (12)

[Claims] 1. An analyzer provided with an on-off valve for controlling gas supply in the middle of a pipe connecting a gas supply source and a reagent solution, wherein an area on the on-off valve side between the on-off valve and the reagent solution is airtight. An analyzer characterized by comprising a pipe made of a highly-reactive material. 2. An analyzer according to claim 1, wherein said highly airtight pipe is a stainless steel pipe. 3. An analyzer according to claim 1, wherein said highly airtight pipe is a polyetheretheretherketone pipe. 4. The analyzer according to claim 1, wherein the inside of the reagent container is pressurized with an inert gas. 5. The analyzer according to claim 1, wherein the reagent is ninhydrin and the device is an amino acid analyzer. 6. A sealable reagent container containing a reagent solution,
A reagent stirring gas supply pipe for supplying a stirring gas into the reagent solution contained in the reagent container, a stirring gas supply control opening / closing valve interposed in the middle of the reagent stirring gas supply pipe, and the reagent container A reagent pressurizing gas supply pipe for supplying a reagent pressurizing gas to an inner space thereof, and a reagent pressurizing gas is supplied into the reagent container in a state where the reagent container is sealed to pressurize the reagent liquid in the reagent container. In the analyzer as described above, a region of the reagent stirring gas supply pipe connecting between the reagent container and the stirring gas supply control opening / closing valve on the connection side with the stirring gas supply control opening / closing valve is connected to the stirring gas. An analyzer comprising a tube made of a highly airtight material. 7. The reagent stirring gas supply pipe connecting the reagent container and the stirring gas supply control opening / closing valve, wherein a region in contact with the reagent in the reagent container is a material which is stable with respect to the reagent liquid. An analyzer characterized by comprising a tubular member. 8. A sealable reagent container containing a reagent solution,
A pressurized gas generating source for generating a pressurized gas, and a reagent agitator for supplying a pressurized gas supplied from the pressurized gas source via a pressurized gas supply pipe into a reagent solution contained in the reagent container A gas supply pipe, a reagent supply gas supply pipe for supplying a pressurized gas supplied via the pressurized gas supply pipe to a space of the reagent container, and the pressurized gas in a state where the reagent container is opened; The reagent agitation gas supply pipe blows into the reagent liquid and stirs the gas. The reagent container is sealed, and the reagent container is sealed. An analyzer in which a reagent solution is pressurized, wherein a gas supply control opening / closing valve is provided in the middle of the pressurized gas supply pipe and the middle of the reagent pressurization gas supply pipe. 9. An analyzer according to claim 8, wherein said reagent stirring gas supply pipe is a Teflon tube. 10. A reagent solution is contained in a sealable reagent container,
With the reagent container open, a reagent stirring gas is blown into the reagent solution through the reagent stirring gas supply pipe to stir the reagent solution, and a reagent pressurizing gas is supplied into the reagent container with the reagent container sealed. An analyzer for pressurizing the reagent solution, further comprising a gas replenishing means for supplying a reagent pressurizing gas to the reagent stirring gas supply pipe. 11. An analyzer according to claim 10, wherein said gas replenishing means includes an on-off valve for supplying a reagent pressurizing gas to said reagent stirring gas supply pipe. 12. A sealable reagent container for accommodating a reagent solution, a reagent stirring gas supply pipe for supplying a stirring gas into the reagent solution accommodated in the reagent container, and a midway of the reagent stirring gas supply pipe. A stirring gas supply control opening / closing valve interposed in the reagent container, and a reagent pressurizing gas supply pipe for supplying a reagent pressurizing gas to a space in the reagent container, wherein the reagent is contained in the reagent container with the reagent container sealed. An analyzer in which a pressurizing gas is supplied to pressurize a reagent solution in the reagent container, wherein the stirring gas supply control on / off valve is opened even in the reagent pressurizing gas for pressurizing the reagent container. An analyzer, comprising: a control device for performing analysis.