JPH10313887A - Production of lactic acid from organic waste - Google Patents
Production of lactic acid from organic wasteInfo
- Publication number
- JPH10313887A JPH10313887A JP9148599A JP14859997A JPH10313887A JP H10313887 A JPH10313887 A JP H10313887A JP 9148599 A JP9148599 A JP 9148599A JP 14859997 A JP14859997 A JP 14859997A JP H10313887 A JPH10313887 A JP H10313887A
- Authority
- JP
- Japan
- Prior art keywords
- lactic acid
- organic waste
- organic
- waste
- bacteria
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Processing Of Solid Wastes (AREA)
Abstract
Description
【0001】[0001]
【発明の属する技術分野】本発明は、家庭、集合住宅、
ホテル、飲食店、給食産業、自治体、食品産業、酒類飲
料産業、乳業産業、廃液処理汚泥等から排出される有機
性廃棄物を原料とする乳酸の製造方法に関する。TECHNICAL FIELD The present invention relates to homes, apartments,
The present invention relates to a method for producing lactic acid from organic waste discharged from hotels, restaurants, catering industry, municipalities, food industry, alcoholic beverage industry, dairy industry, waste liquid treatment sludge, and the like.
【0002】[0002]
【従来の技術】現在、有効資源を種々含む家庭、集合住
宅、ホテル、飲食店、給食産業、自治体、食品産業、酒
類飲料産業、乳業産業、廃液処理汚泥等から排出される
有機性廃棄物は主として焼却、埋め立て、海洋投棄によ
り処理されている。また,場合によっては、そのまま廃
棄せず、コンポスト(堆肥)あるいは家畜飼料としてリ
サイクルする方法も提案されている。一方、有機性廃棄
物を適宜混合し、所望の有機酸を製造する技術も開発さ
れている。2. Description of the Related Art At present, organic waste discharged from households, apartment houses, hotels, restaurants, catering industry, municipalities, food industry, liquor and beverage industry, dairy industry, waste liquid treatment sludge and the like containing various effective resources is known. It is mainly treated by incineration, landfill and ocean dumping. Also, in some cases, a method has been proposed in which compost (compost) or livestock feed is not directly discarded and recycled. On the other hand, a technique for producing a desired organic acid by appropriately mixing organic waste has also been developed.
【0003】[0003]
【発明が解決しようとする課題】しかしながら、有機性
廃棄物を埋め立てにより処理する場合、埋め立て地不足
の問題があり、焼却処理を行うと大型の設備が必要で、
更に悪臭が発生すると言う問題が生じる。また、有機性
廃棄物を堆肥あるいは飼料としてリサイクルする方法
は、処理に時間がかかり、これらの需要と処理すべき有
機性廃棄物量のアンバランスや付加価値の低さのため、
有効な解決策にはなっていない。However, when treating organic waste by landfill, there is a problem of lack of landfills, and incineration requires large facilities.
Further, there is a problem that an odor is generated. In addition, the method of recycling organic waste as compost or feed requires a long processing time, because of the imbalance between these demands and the amount of organic waste to be treated, and low added value.
Not a valid solution.
【0004】一方、乳酸は化学、食品産業において、様
々な有用物質の原料として、又は、プロセス処理成分と
して多くの需要をもち、特に自然環境中で分解される生
分解性プラスチックの原料として利用出来るので、その
生産コストの低減が切望されている。しかし、既往の有
機性廃棄物から有機酸を製造する方法では、生ゴミ等の
有機成分の組成を有機酸製造に適した成分になるように
混合する必要があり、たとえば、有機酸製造に適さない
組成の有機性廃棄物が生ごみとして排出される場合は、
これ単独からは乳酸が生産できない問題があった。本発
明はかかる事情に鑑みてなされたもので、大量に廃棄さ
れる有機性廃棄物から生分解性プラスチックの原料とし
て利用できる乳酸を効率的に製造する有機性廃棄物を原
料とする乳酸の製造方法を提供することを目的とする。On the other hand, lactic acid has a great demand in the chemical and food industries as a raw material for various useful substances or as a component for process treatment, and can be used as a raw material for biodegradable plastics which are decomposed in the natural environment. Therefore, reduction of the production cost is eagerly desired. However, in the method of producing an organic acid from existing organic waste, it is necessary to mix the composition of organic components such as garbage so as to be a component suitable for the production of an organic acid. If organic waste with no composition is discharged as garbage,
There was a problem that lactic acid could not be produced from this alone. The present invention has been made in view of the above circumstances, and is a method for efficiently producing lactic acid that can be used as a raw material for biodegradable plastics from a large amount of organic waste that is discarded. The aim is to provide a method.
【0005】[0005]
【課題を解決するための手段】前記目的に沿う請求項1
記載の有機性廃棄物からの乳酸の製造方法は、生ゴミ、
食品工場等からの廃棄物、未利用有機性資源、若しくは
余剰汚泥等の1又は2以上からなって窒素分を含む有機
性廃棄物に、適度の糖質と水を加え、微生物を作用させ
て、高濃度、高選択的に乳酸を生成させることを特徴と
する。請求項2記載の有機性廃棄物からの乳酸の製造方
法は、生ゴミ、食品工場等からの廃棄物、未利用有機性
資源、若しくは余剰汚泥等の1又は2以上からなって窒
素分を含む有機性廃棄物に、適度の糖質と水を加え、更
に、温度、pH、溶存酸素濃度を制御した状態で、微生
物を作用させて、高濃度、高選択的に乳酸を生成させる
ことを特徴とする。ここで、適度の糖質と水を加え、更
に、温度、pH、溶存酸素濃度を制御した状態で微生物
を作用させるとは、微生物の活動に適した条件に、糖
質、水、温度、pH、溶存酸素濃度を制御することであ
る。According to the present invention, there is provided a semiconductor device comprising:
The method for producing lactic acid from the organic waste described is garbage,
Add appropriate sugars and water to organic waste containing one or two or more wastes from food factories, unused organic resources, or excess sludge and containing nitrogen to allow microorganisms to act. Lactic acid is produced at a high concentration and with high selectivity. The method for producing lactic acid from organic waste according to claim 2 comprises one or more of garbage, waste from food factories, unused organic resources, or excess sludge, and contains nitrogen. It is characterized by adding moderate amount of carbohydrate and water to organic waste, and furthermore, by controlling the temperature, pH and dissolved oxygen concentration, the microorganisms act to produce lactic acid with high concentration and high selectivity. And Here, adding a suitable amount of carbohydrate and water, and further causing the microorganism to act while controlling the temperature, pH, and dissolved oxygen concentration means that conditions suitable for the activity of the microorganism include carbohydrate, water, temperature, and pH. And controlling the dissolved oxygen concentration.
【0006】請求項3記載の有機性廃棄物からの乳酸の
製造方法は、請求項1又は2記載の方法において、前記
微生物には、乳酸菌、又は、乳酸菌と乳酸菌の活動を助
ける菌を含む。ここで、乳酸菌の活動を助ける菌とは、
例えば加水分解菌等の乳酸醗酵に寄与する菌をいう。According to a third aspect of the present invention, there is provided a method for producing lactic acid from organic waste, wherein the microorganisms include a lactic acid bacterium or a lactic acid bacterium and a bacterium which assists the activity of the lactic acid bacterium. Here, bacteria that help the activity of lactic acid bacteria
For example, it refers to bacteria that contribute to lactic acid fermentation such as hydrolytic bacteria.
【0007】請求項4記載の有機性廃棄物からの乳酸の
製造方法は、生ゴミ、食品工場等からの廃棄物、未利用
有機性資源、若しくは余剰汚泥等の1又は2以上からな
って窒素分を含む有機性廃棄物Aに対して、グルコー
ス、シュークロース、デンプン等の糖質Bと、水Cとを
A=(1〜10)×B、C=(0.3〜5)×(A+
B)の重量割合で添加し、常温から55℃の温度を保つ
と共に、pHを3を超えて12以下で、しかも嫌気性状
態を適宜保ちながら、前記有機性廃棄物に自然に付着す
る微生物を作用させて、高濃度でしかも高選択的に乳酸
を生成させることを特徴とする。なお、ここで、有機性
廃棄物は水分を含んだ状態の湿潤重量として計算する。
有機性廃棄物Aに対して、グルコース、シュークロー
ス、デンプン等の糖質Bと、水Cとを〔A=(1〜1
0)×B〕の割合で添加とは、有機性廃棄物に糖質を重
量で糖質1に対し、有機性廃棄物を1から10の割合で
添加することをいう。これは、糖質1に対して有機性廃
棄物が1未満であると、不経済であり、又乳酸菌の活性
も悪くなるし、10より多いと乳酸菌の活性が非常に低
下し、高濃度の乳酸の生成が望めなくなるからであり、
糖質1に対し有機性廃棄物を3から8にするのが好まし
い。また、〔C=(0.3〜5)×(A+B)〕の重量
割合とは、有機性廃棄物と糖質の重量合計を1として、
0.3〜5倍の水分を含むようにすることをいう。これ
は水分が30%未満であると、乳酸菌に対して適度な水
分活性が無くなり、水分量が5倍より多くなると、他の
雑菌の繁殖がおこるという問題が生じ、生成される乳酸
が低濃度となるからであり、水分量を0.5〜3倍にす
るのが好ましく、水、あるいは水溶性溶液を添加して不
足量を補うようにする。[0007] The method for producing lactic acid from organic waste according to claim 4 is a method for producing lactic acid from one or more of garbage, waste from food factories, unused organic resources, and excess sludge. Of organic waste A containing glucose, saccharide B such as glucose, sucrose and starch, and water C as follows: A = (1-10) × B, C = (0.3-5) × ( A +
B) at a weight ratio of (B), keeping the temperature from room temperature to 55 ° C., and maintaining the pH above 3 to 12 and below, and appropriately maintaining the anaerobic state, and removing microorganisms that naturally adhere to the organic waste. Lactic acid is produced at a high concentration and selectively. Here, the organic waste is calculated as a wet weight containing water.
To the organic waste A, a sugar B such as glucose, sucrose, starch, etc. and water C were added [A = (1-1)
0) × B] means that the organic waste is added to the organic waste at a ratio of 1 to 10 with respect to the sugar 1 by weight. This is uneconomical if the organic waste is less than 1 per carbohydrate, and the activity of lactic acid bacteria is worsened. If it is more than 10, the activity of lactic acid bacteria is greatly reduced and the concentration of Because the production of lactic acid can no longer be expected,
It is preferable to use 3 to 8 organic wastes per carbohydrate. Further, the weight ratio of [C = (0.3-5) × (A + B)] is defined assuming that the total weight of the organic waste and the saccharide is 1,
It means to contain 0.3 to 5 times water. This is because if the water content is less than 30%, the water activity of the lactic acid bacteria is lost, and if the water content is more than 5 times, there is a problem that other bacteria grow. It is preferable to increase the water content by 0.5 to 3 times, and water or a water-soluble solution is added to compensate for the shortage.
【0008】さらに、常温から55℃の温度を保つと共
に、pHを3を超えて12以下で、しかも嫌気性状態を
適宜保ちながら、微生物を好適に作用させて乳酸を生成
するとは、雰囲気液を乳酸菌の活性に適した条件を整え
ることであり、雰囲気液の温度は常温〜55℃、より効
果的には20℃〜50℃の範囲に制御するのが好まし
い。また、pHを3を超えて12以下に保つのは、pH
が3以下の場合、又はpHが12を越える場合には、雰
囲気液の酸性、アルカリ性が強すぎて、微生物の活性が
低下、あるいは死滅するためであり、微生物の活動に適
した条件にpHを調節するのが好ましく、pHを5〜1
0の範囲に制御するのがより好ましい。また、ここで嫌
気性状態を適宜保つとは、通気と嫌気状態を調節して乳
酸の活動に適した主に嫌気状態にすることの他、常時、
嫌気状態を維持することも含む。前記有機性廃棄物に自
然に付着する微生物とは、特別に菌を他から加えずとも
自然に有機性廃棄物に付着、混在する菌のことであり、
例えば、種々の乳酸菌、加水分解菌、乳酸以外の酸を生
成する酸生成菌等の種々の菌が有機性廃棄物に付着して
存在し、乳酸醗酵に好適な条件を整えることにより、他
から菌を添加せずに、高選択的、高濃度に乳酸醗酵させ
ることができる。具体的には例えば、コリネバクテリウ
ム(Corynebacterium)、ラクトバシラ
ス(Lactobacillus)、ラミバクテリウム
(Ramibacterium)、アクチノマイセス
(Actinomyces)、ビフィドバクテリウム
(Bifidobacterium)、バシラス(Ba
cillus)、ミクロコッカス(Micrococc
us)、シュードモナス(Pseudomonas)等
の菌であり、これらには加水分解、酸生成の両方の働き
をするものも多い。Further, to maintain the temperature from room temperature to 55 ° C., to maintain the pH above 3 and below 12 and to appropriately maintain the anaerobic state, and to appropriately act on microorganisms to produce lactic acid means that It is to prepare conditions suitable for the activity of lactic acid bacteria, and it is preferable to control the temperature of the atmosphere liquid to the range of normal temperature to 55 ° C, more effectively 20 ° C to 50 ° C. Also, maintaining the pH above 3 and below 12 is due to the pH
When the pH is 3 or less, or when the pH exceeds 12, the acidity or alkalinity of the atmosphere liquid is too strong, and the activity of the microorganisms is reduced or killed. The pH is adjusted to a condition suitable for the activity of the microorganisms. It is preferable to adjust the pH to 5 to 1
It is more preferable to control the value in the range of 0. In addition, to maintain the anaerobic state appropriately here, in addition to adjusting the ventilation and anaerobic state to make it mainly anaerobic state suitable for the activity of lactic acid,
This includes maintaining anaerobic conditions. The microorganism that naturally adheres to the organic waste is a microorganism that naturally adheres to and mixes with the organic waste without any special addition of bacteria.
For example, various lactic acid bacteria, hydrolytic bacteria, various bacteria such as acid-producing bacteria that produce acids other than lactic acid are present attached to organic waste, and by setting conditions suitable for lactic acid fermentation, Highly selective and high-concentration lactic acid fermentation can be performed without adding bacteria. Specifically, for example, Corynebacterium, Lactobacillus, Lamibacterium, Actinomyces, Bifidobacterium, Bacillus (Babacillus)
C., micrococcus (Micrococc)
), Pseudomonas and the like, and many of them have both functions of hydrolysis and acid generation.
【0009】請求項5記載の有機性廃棄物からの乳酸の
製造方法は、請求項4記載の乳酸の製造方法において、
前記微生物に、乳酸菌及び/又は乳酸菌の活動を助ける
菌を加えることを特徴とする。請求項6記載の有機性廃
棄物からの乳酸の製造方法は、請求項1〜5のいずれか
1項に記載の乳酸の製造方法において、前記微生物に加
えて、乳酸醗酵させた汚泥滓及び/又は余剰汚泥を投入
することを特徴とする。これは、例えば工場よりオンラ
インで直送された等の、自然に付着、混在している菌が
非常に少ない有機性廃棄物に対しては、乳酸菌、加水分
解菌、乳酸菌と加水分解菌の混合、あるいは菌の発生の
多く見られる乳酸醗酵させた汚泥滓及び/又は余剰汚泥
等を投入して、乳酸醗酵の条件をより整えることをい
う。請求項1〜6記載の乳酸の製造方法において糖質と
は、グルコース、シュークロース、デンプンなど単糖、
オリゴ糖、多糖類などいずれの糖でも使用可能である。
これは、乳酸を生成する材料として有機性廃棄物を使用
するため、有機性廃棄物には乳酸菌だけではなく加水分
解菌等、種々の菌が自然に付着、混在しているので、デ
ンプンなどの多糖類であっても加水分解菌により分解さ
れて、乳酸菌の活性化に寄与するからである。但し、自
然に付着、混在している菌が非常に少ない有機性廃棄物
に対しては、乳酸菌、加水分解菌、あるいは菌の発生の
多く見られる乳酸醗酵させた汚泥滓及び/又は余剰汚泥
等を投入して、乳酸醗酵の条件をより整えて作用させる
のが好ましい。The method for producing lactic acid from organic waste according to claim 5 is a method for producing lactic acid according to claim 4,
A lactic acid bacterium and / or a bacterium that assists the activity of the lactic acid bacterium is added to the microorganism. The method for producing lactic acid from organic waste according to claim 6 is the method for producing lactic acid according to any one of claims 1 to 5, wherein, in addition to the microorganisms, lactic acid-fermented sludge and / or Alternatively, surplus sludge is introduced. This is because, for example, organic waste with very few bacteria that are naturally attached and mixed, such as those sent directly from factories online, are lactic acid bacteria, hydrolytic bacteria, a mixture of lactic acid bacteria and hydrolytic bacteria, Alternatively, it means that lactic acid-fermented sludge slag and / or surplus sludge, which are often found in the generation of bacteria, are introduced to further adjust the conditions of lactic acid fermentation. In the method for producing lactic acid according to claims 1 to 6, the saccharide is a monosaccharide such as glucose, sucrose, and starch;
Any sugars such as oligosaccharides and polysaccharides can be used.
This is because organic waste is used as a material for producing lactic acid, and not only lactic acid bacteria but also various bacteria such as hydrolytic bacteria naturally adhere to and mix with organic waste. This is because even polysaccharides are decomposed by hydrolytic bacteria and contribute to activation of lactic acid bacteria. However, lactic acid bacteria, hydrolyzing bacteria, or lactic acid-fermented sludge and / or excess sludge, which often generate bacteria, are used for organic wastes that have very few bacteria attached and mixed naturally. It is preferred that the lactic acid fermentation conditions be adjusted to make the lactic acid fermentation work.
【0010】請求項1〜6において、乳酸の生成が高濃
度であるとは10g/L以上、高選択的とは、有機酸あ
たりの乳酸の割合が80%以上であることをいう。ま
た、窒素分を含む有機性廃棄物とは、有機性廃棄物に窒
素分が含まれない場合には、窒素分を有機性廃棄物の例
えば1〜5%入れることをいい、例えば、窒素分を含む
他の有機性廃棄物、乳酸醗酵させた汚泥滓及び/又は余
剰汚泥を投入することにより窒素分を含ませることがで
きる。これは、窒素分がない場合には乳酸菌の活性が非
常に低下し、高濃度の乳酸の生成が望めなくなるからで
ある。ここで、未利用有機性資源とは、未利用のまま、
あるいは利用はされたが未だ価値のある状態で廃棄物と
された有機性資源のことをいう。In claims 1 to 6, high concentration of lactic acid means 10 g / L or more, and high selectivity means that the ratio of lactic acid per organic acid is 80% or more. Further, the organic waste containing nitrogen means that when the organic waste does not contain nitrogen, 1 to 5% of nitrogen is added to the organic waste. Nitrogen can be contained by introducing other organic waste containing, fermented lactic acid fermented sludge and / or excess sludge. This is because in the absence of nitrogen, the activity of the lactic acid bacterium is greatly reduced, and the production of high-concentration lactic acid cannot be expected. Here, the unused organic resources are not used,
Alternatively, it refers to organic resources that have been used but have been discarded in a valuable state.
【0011】[0011]
【発明の実施の形態】本発明は有機性廃棄物から乳酸を
製造する方法に関するものであり、以下に本発明の乳酸
の製造方法の作用、効果を確認するために行った実験例
について説明する。実験にあたっては、食品工場からの
有機性廃棄物の例であり、未利用有機性資源でもあるか
つお出汁粕と昆布出汁粕の混合物、かつお出汁粕としい
たけ出汁粕の混合物、小豆殻を試料として実験を行っ
た。また、醸造工場からの廃棄物の例である米焼酎廃液
粕、いも焼酎廃液粕、ビール工場からのビール麦汁粕を
試料として実験を行った。さらに、余剰汚泥の一例であ
る牛乳工場の排水処理池起源の余剰汚泥を試料として実
験した。本発明方法の糖質添加の効果をみるために、実
験例1、実験例2、実験例3にて、有機性廃棄物に糖質
として単糖であるグルコース、二糖であるシュークロー
ス、多糖であるデンプンを加えた場合と糖質を何も加え
ない場合について各々、比較検討した。また、有機性廃
棄物と糖質の合計の5倍より多く水分を含有する場合に
ついて実験例6にて検討した。さらに、有機性廃棄物に
作用する菌として、有機性廃棄物に自然に付着、混在す
る菌ではなく、工業的に広く用いられている乳酸菌であ
るラクトバシラス デルブルエキー(Lactobac
illus delbrueckii)を作用させた場
合についても実験例7にて比較検討した。DESCRIPTION OF THE PREFERRED EMBODIMENTS The present invention relates to a method for producing lactic acid from organic wastes. Hereinafter, experimental examples performed to confirm the operation and effects of the method for producing lactic acid of the present invention will be described. . In the experiment, a mixture of bonito soup cake and kelp soup cake, which is an example of organic waste from a food factory and also an unused organic resource, a mixture of bonito soup cake and shiitake soup cake, and red bean shell were used as samples. Was done. In addition, an experiment was performed using rice shochu waste liquid lees, potato shochu waste liquid lees, and beer wort lees from a beer factory as examples of wastes from a brewery. Furthermore, an experiment was conducted using a sample of excess sludge originating from a wastewater treatment pond of a milk factory, which is an example of excess sludge. In order to examine the effect of the addition of carbohydrates in the method of the present invention, in Experimental Examples 1, 2, and 3, glucose, which is a monosaccharide, sucrose, which is a disaccharide, and polysaccharide, were used as organic carbohydrates. And a case where no carbohydrate was added was compared and examined. Further, a case in which water was contained more than 5 times the total of the organic waste and the saccharide was examined in Experimental Example 6. Furthermore, as a bacterium acting on organic waste, it is not a bacterium which naturally adheres to and mixes with organic waste, but is a lactic acid bacterium, Lactobacillus delbrueque, which is widely used industrially.
Comparative study was also carried out in Experimental Example 7 in the case where illus delbrueckii) was allowed to act.
【0012】(実験例1)食品工場からの有機性廃棄物
である、かつおぶしと昆布の1対1に、2%から20%
になるように水を加え沸騰させて出汁を取った後の出汁
粕、を試料として用いた。この出汁粕8gに対し、グル
コース、シュークロース、デンプンをそれぞれ2gずつ
加え、各々10gとした3種類、及び糖質を加えず出汁
粕10gのみの4種類の固形試料にそれぞれ合計で30
gになるよう水を加え、50mlの遠心管に入れ、37
℃の恒温槽中で発酵させた。初期pHを10とし、12
時間毎にpHを10に戻した。発酵72時間後に遠心管
を遠心し、固形分を沈澱させた後、液相部分を回収し、
液相部分の乳酸濃度を測定した。(Experimental Example 1) 2% to 20% of one-to-one organic waste from a food factory, bonito and kelp
The soup stock obtained after adding water and boiling to remove the soup was used as a sample. To 8 g of the dashi cake, glucose, sucrose and starch were added in an amount of 2 g each, and 3 types of 10 g each were added.
g, add water to a 50 ml centrifuge tube, and add
The fermentation was carried out in a constant temperature bath at 0 ° C. Initial pH is 10 and 12
The pH was returned to 10 every hour. After 72 hours of fermentation, the centrifuge tube was centrifuged to precipitate solids, and the liquid phase was recovered.
The lactic acid concentration in the liquid phase was measured.
【0013】[0013]
【表1】 [Table 1]
【0014】表1に示すように、グルコース、シューク
ロース、デンプンの何れの糖質を添加した分からも、乳
酸が高濃度、高選択的に生成されているが、糖質を添加
しない比較例からは殆ど乳酸ができていないことがわか
る。As shown in Table 1, lactic acid is produced in a high concentration and in a high selectivity from the addition of glucose, sucrose, and starch, and the comparative example in which no sugar is added. Shows that almost no lactic acid is formed.
【0015】(実験例2)食品工場からの有機性廃棄物
である、かつおぶしとしいたけを1対1に加え、2%か
ら20%になるよう水を加え沸騰させて出汁を取った後
の出汁粕、を試料として用いた。この出汁粕8gに対
し、グルコース、シュークロース、デンプンをそれぞれ
2gずつ加え、各々10gとした3種類、及び糖質を加
えず出汁粕10gのみの4種類の固形試料に、それぞれ
合計で30gになるよう水を加え、50mlの遠心管に
入れ、37℃の恒温槽中で発酵させた。初期pHを10
とし、12時間毎にpHを10に戻した。発酵72時間
後に遠心管を遠心し、固形分を沈澱させた後、液相部分
を回収し、液相部分の乳酸濃度を測定した。(Experimental Example 2) Dashi after removing the soup by adding water to 2 to 20% and adding water so as to make it 2 to 20%, which is an organic waste from a food factory. Cake was used as a sample. Glucose, sucrose, and starch were added to 8 g of this dashi cake, respectively, and 2 g of each was added, and 3 types of 10 g were added to each, and 4 types of solid samples of only 10 g of dashi cake without adding carbohydrate were each added to a total of 30 g. Iodine water was added, put in a 50 ml centrifuge tube, and fermented in a thermostat at 37 ° C. Initial pH 10
And the pH was returned to 10 every 12 hours. After 72 hours of fermentation, the centrifuge tube was centrifuged to precipitate solids, then the liquid phase was recovered, and the lactic acid concentration in the liquid phase was measured.
【0016】[0016]
【表2】 [Table 2]
【0017】表2に示すように、グルコース、シューク
ロース、デンプンの何れの糖質を添加した分からも、乳
酸が高濃度、高選択的に生成されているが、糖質を添加
しない比較例からは殆ど乳酸ができていないことがわか
る。As shown in Table 2, the lactic acid was produced in a high concentration and in a highly selective manner from the addition of any of the carbohydrates of glucose, sucrose, and starch. Shows that almost no lactic acid is formed.
【0018】(実験例3)あんこを製造するために加工
された食品工場からの有機性廃棄物である小豆の殻を試
料として用いた。この小豆殻8gに対し、グルコース、
シュークロース、デンプンをそれぞれ2gずつ加え、各
々10gとした3種類、及び糖質を加えず小豆殻10g
のみの4種類の固形試料にそれぞれ、合計で30gにな
るように水を加え、50mlの遠心管に入れ、37℃の
恒温槽中で発酵させた。初期pHを10とし、12時間
毎にpHを10に戻した。発酵72時間後に遠心管を遠
心し、固形分を沈殿させた後、液相部分を回収し、液相
部分の乳酸濃度を測定した。(Experimental Example 3) Red bean husk, which is an organic waste from a food factory processed for producing bean paste, was used as a sample. For 8 g of this red bean shell, glucose,
Sucrose and starch were added in 2g each, 3g each in 10g, and 10g red bean hull without sugar
Water was added to each of the four solid samples to a total of 30 g, placed in a 50 ml centrifuge tube, and fermented in a 37 ° C. thermostat. The initial pH was set to 10, and the pH was returned to 10 every 12 hours. After 72 hours of fermentation, the centrifuge tube was centrifuged to precipitate solids, then the liquid phase was recovered, and the lactic acid concentration in the liquid phase was measured.
【0019】[0019]
【表3】 [Table 3]
【0020】表3に示すように、有機性廃棄物に小豆殻
を使用した本実験例では、乳酸生成量が他の実験例に比
較して少ないが、使用した有機性廃棄物である小豆殻に
は、含有される窒素分が少ないためと考えられる。それ
でもグルコース、シュークロース、デンプンの何れの糖
質を添加した分からも、生成された他の有機酸である酢
酸、プロピオン酸、蟻酸に比較すると乳酸が10g/l
以上で、10倍以上の高濃度で選択的に生成されてい
る。一方、糖質を添加していない比較例からは殆ど乳酸
ができていない。As shown in Table 3, in this experimental example using red bean husks as the organic waste, although the amount of lactic acid produced was smaller than in other experimental examples, the red bean husk used as the organic waste was used. Is thought to be due to the low nitrogen content. Nevertheless, the amount of lactic acid is 10 g / l in comparison with the other organic acids produced, acetic acid, propionic acid and formic acid, from the addition of glucose, sucrose and starch.
As described above, it is selectively generated at a high concentration of 10 times or more. On the other hand, lactic acid was hardly produced from the comparative example to which no saccharide was added.
【0021】(実験例4)米焼酎廃液粕の懸濁固形分を
試料として用いた。この米焼酎粕8gに対し、グルコー
ス、シュークロース、デンプンをそれぞれ2gずつ加
え、各々10gとした3種類の固形試料にそれぞれ合計
で30gになるように水を加え、50mlの遠心管に入
れ、37℃の恒温槽中で発酵させた。初期pHを10と
し、12時間毎にpHを10に戻した。発酵72時間後
に遠心管を遠心し、固形分を沈殿させた後、液相部分を
回収し、液相部分の乳酸濃度を測定した。(Experimental Example 4) A suspended solid content of rice shochu waste liquid lees was used as a sample. To 8 g of the rice shochu lees, 2 g each of glucose, sucrose, and starch were added, and water was added to each of the three types of solid samples, each of which was 10 g, to a total of 30 g. The fermentation was carried out in a constant temperature bath at 0 ° C. The initial pH was set to 10, and the pH was returned to 10 every 12 hours. After 72 hours of fermentation, the centrifuge tube was centrifuged to precipitate solids, then the liquid phase was recovered, and the lactic acid concentration in the liquid phase was measured.
【0022】[0022]
【表4】 [Table 4]
【0023】表4に示すように、米焼酎滓を使用した本
実験例では、乳酸生成量が他の実験例に比較して少ない
が、米焼酎滓には窒素分の含有が少ないためと考えられ
る。それでもグルコース、シュークロース、デンプンの
何れの糖質を添加した分からも、生成された他の有機酸
である酢酸、プロピオン酸、蟻酸、酪酸に比較すると乳
酸が10g/l以上で、10倍以上の高濃度で高選択的
に生成されていることがわかる。As shown in Table 4, in this experimental example using rice shochu slag, the amount of lactic acid produced was smaller than in the other experimental examples. Can be Nevertheless, even if glucose, sucrose or starch is added, lactic acid is more than 10 g / l and more than 10 times as much as acetic acid, propionic acid, formic acid and butyric acid, which are other organic acids produced. It can be seen that it is produced with high concentration and high selectivity.
【0024】(実験例5)芋焼酎廃液粕の懸濁固形分を
試料として用いた。この焼酎粕8gに対し、グルコー
ス、シュークロース、デンプンをそれぞれ2gずつ加
え、各々10gとした3種類の固形試料にそれぞれ合計
で30gになるように水を加え、50mlの遠心管に入
れ、37℃の恒温槽中で発酵させた。初期pHを10と
し、12時間毎にpHを10に戻した。発酵72時間後
に遠心管を遠心し、固形分を沈殿させた後、液相部分を
回収し、液相部分の乳酸濃度を測定した。表5に示すよ
うに、いずれの糖質を添加した場合でも、乳酸が高濃
度、高選択的に生成されていることがわかる。(Experimental Example 5) A suspended solid content of potato shochu waste liquid lees was used as a sample. To 8 g of this shochu lees, 2 g each of glucose, sucrose, and starch were added, and water was added to each of the three types of solid samples of 10 g each so that the total amount became 30 g. Fermentation in a thermostat. The initial pH was set to 10, and the pH was returned to 10 every 12 hours. After 72 hours of fermentation, the centrifuge tube was centrifuged to precipitate solids, then the liquid phase was recovered, and the lactic acid concentration in the liquid phase was measured. As shown in Table 5, it can be seen that lactic acid was produced at a high concentration and with high selectivity, regardless of the type of saccharide added.
【0025】[0025]
【表5】 [Table 5]
【0026】(実験例6)次に、芋焼酎廃液粕の懸濁固
形分を試料として、水の含有量を変えた実験を行った。
焼酎粕2gに対し、グルコース、シュークロース、デン
プンをそれぞれ0.3gずつ加え、各々2.3gとした
3種類の固形試料にそれぞれ合計で30gになるように
水を加え、50mlの遠心管に入れ、37℃の恒温槽中
で発酵させた。初期pHを10とし、12時間毎にpH
を10に戻した。発酵72時間後に遠心管を遠心し、固
形分を沈殿させた後、液相部分を回収し、液相部分の乳
酸濃度を測定した。(Experimental Example 6) Next, an experiment was conducted in which the content of water was changed using a suspended solid content of potato shochu waste liquid lees as a sample.
To 2 g of shochu lees, add 0.3 g each of glucose, sucrose, and starch, and add water to each of the three types of solid samples of 2.3 g each so that the total amount becomes 30 g, and put into a 50 ml centrifuge tube. , And fermented in a thermostat at 37 ° C. The initial pH is 10 and the pH is adjusted every 12 hours.
Was returned to 10. After 72 hours of fermentation, the centrifuge tube was centrifuged to precipitate solids, then the liquid phase was recovered, and the lactic acid concentration in the liquid phase was measured.
【0027】[0027]
【表6】 [Table 6]
【0028】実験例5と実験例6では同じ芋焼酎滓、同
じ糖質を使用し、有機性廃棄物及び糖質に対する水の含
有量を変えて実験を行ったが、表6に示すように、水の
含有量が、有機性廃棄物及び糖質の約12倍になる比較
例では乳酸は非常に低濃度にしかできていないことがわ
かる。In Experiments 5 and 6, the same potato shochu sac and the same carbohydrate were used, and the experiment was conducted by changing the content of water with respect to the organic waste and the carbohydrate. In the comparative example in which the water content is about 12 times that of the organic waste and saccharide, it can be seen that lactic acid is formed only at a very low concentration.
【0029】(実験例7)次に本発明の効果をみるため
に、比較例として、有機性廃棄物に自然に付着、混在す
る菌を作用させず、乳酸菌(ラクトバシラス デルブル
エキー)を作用させた場合について以下に実験した。芋
焼酎廃液粕の懸濁固形分を蒸気滅菌したものを試料とし
て用いた。この焼酎粕8gに対し、グルコース、ラクト
ース、デンプンをそれぞれ2gずつ加え、各々10gと
した3種類の固形試料にそれぞれ合計で30gになるよ
うに水を加え、50mlの遠心管に入れ工業的に広く用
いられている乳酸菌の溶液を0.1%添加し、37℃の
恒温槽中で発酵させた。初期pHを10とし、12時間
毎にpHを10に戻した。発酵72時間後に遠心管を遠
心し、固形分を沈殿させた後、液相部分を回収し、液相
部分の乳酸濃度を測定した。(Experimental Example 7) Next, in order to see the effect of the present invention, as a comparative example, a case where lactic acid bacteria (Lactobacillus delbruecki) were allowed to act without causing bacteria that naturally adhered and mixed with organic waste to act. Was tested below. A steam-sterilized suspension solid of potato shochu waste liquid lees was used as a sample. To 8 g of this shochu lees, 2 g each of glucose, lactose, and starch were added, and water was added to each of the three types of solid samples, each of which was 10 g, to a total of 30 g. 0.1% of the used lactic acid bacterium solution was added, and the mixture was fermented in a thermostat at 37 ° C. The initial pH was set to 10, and the pH was returned to 10 every 12 hours. After 72 hours of fermentation, the centrifuge tube was centrifuged to precipitate solids, then the liquid phase was recovered, and the lactic acid concentration in the liquid phase was measured.
【0030】[0030]
【表7】 [Table 7]
【0031】その結果を以下に示すが、糖質としてデン
プンを用いた場合には全く乳酸が生成されていない。こ
のことより、有機性廃棄物を蒸気滅菌し、その後、工業
的に広く用いられている乳酸菌を添加した場合には、糖
質としては、単糖であるグルコースと二糖であるラクト
ースしか乳酸醗酵に寄与せず、多糖類であるデンプンは
有機性廃棄物に混在する加水分解菌の作用を受けていな
いため、乳酸醗酵に寄与してないと思われる。The results are shown below. When starch is used as a saccharide, no lactic acid is produced. From this, when organic waste is steam-sterilized and then lactic acid bacteria widely used industrially are added, only saccharides such as glucose and disaccharide lactose are monosaccharides. It does not contribute to lactic acid fermentation because starch, which is a polysaccharide, is not affected by hydrolytic bacteria mixed in organic waste.
【0032】(実験例8)ビール麦汁粕を試料として用
いた。この麦汁粕8gに対し、グルコース、シュークロ
ース、デンプンをそれぞれ2gずつ加え、各々10gと
した3種類の固形試料にそれぞれ合計で30gになるよ
うに水を加え、50mlの遠心管に入れ、37℃の恒温
槽中で発酵させた。初期pHを10とし、12時間毎に
pHを10に戻した。発酵72時間後に遠心管を遠心
し、固形分を沈殿させた後、液相部分を回収し、液相部
分の乳酸濃度を測定した。結果を以下に示すが、乳酸が
高濃度、高選択的に生成していることがわかる。(Experimental Example 8) Beer wort cake was used as a sample. To 8 g of this wort cake, glucose, sucrose, and starch were added in an amount of 2 g each, and water was added to each of the three types of solid samples, each of which was 10 g, to a total of 30 g. The fermentation was carried out in a constant temperature bath at 0 ° C. The initial pH was set to 10, and the pH was returned to 10 every 12 hours. After 72 hours of fermentation, the centrifuge tube was centrifuged to precipitate solids, then the liquid phase was recovered, and the lactic acid concentration in the liquid phase was measured. The results are shown below, and it can be seen that lactic acid is produced at a high concentration and with high selectivity.
【0033】[0033]
【表8】 [Table 8]
【0034】(実験例9)牛乳工場の排水処理池起源の
余剰汚泥を試料として用いた。この余剰汚泥8gに対
し、グルコース、シュークロース、デンプンをそれぞれ
2gずつ加え、各々10gとした3種類の固形試料に、
それぞれ、合計で30gになるように水を加え、50m
lの遠心管に入れ、37℃の恒温槽中で発酵させた。初
期pHを10とし、12時間毎にpHを10に戻した。
発酵72時間後に遠心管を遠心し、固形分を沈殿させた
後、液相部分を回収し、液相部分の乳酸濃度を測定し
た。その結果を以下に示すが、乳酸が高濃度、高選択的
に生成されていることがわかる。(Experimental Example 9) Excess sludge originating from a wastewater treatment pond of a milk factory was used as a sample. To 8 g of the excess sludge, glucose, sucrose, and starch were added in 2 g each, and three types of solid samples each having 10 g were added.
Add water so that the total amount is 30g, and add 50m
and fermented in a 37 ° C. thermostat. The initial pH was set to 10, and the pH was returned to 10 every 12 hours.
After 72 hours of fermentation, the centrifuge tube was centrifuged to precipitate solids, then the liquid phase was recovered, and the lactic acid concentration in the liquid phase was measured. The results are shown below, and it can be seen that lactic acid is produced at a high concentration and with high selectivity.
【0035】[0035]
【表9】 [Table 9]
【0036】上記1〜6、8及び9の実験例において
は、糖質として、グルコース、シュークロース、デンプ
ンを用いて、有機性廃棄物に自然に付着、混在する菌を
作用させたが、いずれの糖質を用いた場合にも、乳酸が
高濃度、高選択に生成されていることがわかる。また、
糖質として、ブラウンシュガーやキャッサバデンプン等
の廉価な原料を用いた場合、廃糖蜜や菓子産業廃棄物等
の糖質を多く含む廃液、廃棄物、これらの混合物を糖質
として使用した場合、あるいは、有機性廃棄物を、物理
的、科学的、あるいは生化学的方法によって一般糖化し
たものを使用した場合についても、本発明方法の適用が
可能であり、本発明方法に含まれる。In the experimental examples 1 to 6, 8 and 9, glucose, sucrose and starch were used as carbohydrates, and bacteria that naturally adhered to and mixed with the organic waste were allowed to act. It can be seen that lactic acid is produced in high concentration and high selectivity even when the saccharide is used. Also,
When low-cost raw materials such as brown sugar and cassava starch are used as saccharides, waste liquids containing a large amount of saccharides such as molasses and confectionery industrial waste, wastes, and mixtures thereof are used as saccharides, or The method of the present invention is also applicable to the case where organic waste is used for general saccharification by physical, scientific or biochemical methods, and is included in the method of the present invention.
【0037】上記1〜6、8及び9の実験例において
は、食品工場、醸造工場からの有機性廃棄物、また、牛
乳工場の排水処理池起源の余剰汚泥に自然に付着、混在
する菌を作用させた場合について実験したが、例えば食
品工場等よりオンラインにより直送されるなどして、自
然に付着、混在する菌が非常に少ない有機性廃棄物の場
合でも、乳酸菌、加水分解菌等の乳酸菌の働きを助ける
菌を添加する、あるいは他の有機性廃棄物、乳酸醗酵さ
せた汚泥滓、余剰汚泥等のいずれか1つあるいは2以上
を入れることにより本発明方法の実施が可能であり、本
発明方法に含まれる。また、有機性廃棄物に窒素分が含
まれない、あるいは微量の場合には、例えば窒素を含有
する有機性廃棄物、乳酸醗酵させた汚泥滓、余剰汚泥等
のいずれか1つあるいは2以上を入れて、窒素分を有機
性廃棄物の例えば1〜5%にすることにより、本発明方
法を適用でき、その場合にも本発明方法に含まれる。上
記1〜9の実験例においては、水の含有量を、有機性廃
棄物及び糖質の約2倍の例、及び12倍の例のみを示し
たが、水の含有量が0.3〜5倍の場合には高濃度、高
選択的に乳酸が生成され、本発明方法に含まれる。In the above experimental examples 1 to 6, 8 and 9, fungi naturally adhering to and mixed with organic wastes from food factories and brewing factories and excess sludge originating from wastewater treatment ponds of milk factories. Although experiments were conducted on the case where it was made to act, for example, lactic acid bacteria such as lactic acid bacteria and hydrolytic bacteria, even when organic waste with very few bacteria attached and mixed naturally, for example, sent directly online from a food factory, etc. It is possible to carry out the method of the present invention by adding a bacterium which assists the action of the above, or by adding any one or more of other organic waste, sludge which has been fermented with lactic acid, and excess sludge. Included in the invention method. In the case where the organic waste does not contain or has a small amount of nitrogen, for example, one or two or more of nitrogen-containing organic waste, lactic acid-fermented sludge, and excess sludge are used. The method of the present invention can be applied by adding the nitrogen content to, for example, 1 to 5% of the organic waste, and the case is also included in the method of the present invention. In the experimental examples 1 to 9 described above, the water content is only about twice as much as the organic waste and the carbohydrate, and only 12 times as much as the water content. In the case of 5 times, lactic acid is produced with high concentration and high selectivity and is included in the method of the present invention.
【0038】上記実験例においては、周知のpH調整剤
により、初期及び12時間毎のpH調整を行ったが、有
機性廃棄物を入れた容器にpHセンサー、温度センサ
ー、溶存酸素濃度センサーをつけ、pH調整装置、加熱
装置、酸素吹き込み装置を取付けて、pH、温度、溶存
酸素濃度の調整を測る場合も本発明に含まれる。In the above experimental example, the pH was adjusted at an initial stage and every 12 hours with a well-known pH adjuster. A pH sensor, a temperature sensor, and a dissolved oxygen concentration sensor were attached to the container containing the organic waste. The present invention also includes a case where a pH, a temperature, and a dissolved oxygen concentration are measured by installing a pH adjusting device, a heating device, and an oxygen blowing device.
【0039】本発明方法によって、有機酸中に高濃度、
高選択的に生成された乳酸は濃縮前、濃縮後に周知の単
独あるいは数種の方法によって、高純度の乳酸に分離精
製される。According to the method of the present invention, a high concentration
Lactic acid produced in a highly selective manner is separated and purified into high-purity lactic acid before and after concentration by a known method or several methods.
【0040】[0040]
【発明の効果】請求項1〜6記載の有機性廃棄物からの
乳酸の製造方法によって、生ゴミ、食品工場等からの廃
棄物、未利用有機性資源、若しくは余剰汚泥等からなる
有機性廃棄物に、適度の糖質と水を加え微生物を作用さ
せることにより、利用価値の高い乳酸を高濃度、高選択
的に生産することができるようになった。そしてこのよ
うにして得られた乳酸からは化学的に、あるいは、醗酵
法によって、生分解性プラスチックが製造できるし、乳
酸そのものを食品添加物としても利用できるし、化学的
処理を加えた後、様々な化学製品の原料として用いるこ
ともができる。さらに、有効資源を種々含みながら、主
として焼却、埋め立て、海洋投棄により処理されていた
有機性廃棄物の廃棄物処理の点からも、大きな役割を果
たすこととなった。特に、請求項2記載の有機性廃棄物
からの乳酸の製造方法は有機性廃棄物に、適度の糖質と
水を加え、更に、温度、pH、溶存酸素濃度を制御した
状態で、微生物を作用させているので、乳酸菌の活動に
適した条件となり、高濃度、高選択的に乳酸が生成でき
る。請求項3記載の有機性廃棄物からの乳酸の製造方法
は微生物に、乳酸菌、又は乳酸菌と乳酸菌の活動を助け
る菌を含むので、高濃度、高選択的に乳酸が生成でき
る。請求項4記載の有機性廃棄物からの乳酸の製造方法
は、生ゴミ、食品工場等からの廃棄物、未利用有機性資
源、若しくは余剰汚泥等の1又は2以上からなって窒素
分を含む有機性廃棄物Aに対して、グルコース、シュー
クロース、デンプン等の糖質Bと、水Cとを以下の割合
で添加し、常温から55℃の温度を保つと共に、pHを
3を超えて12以下で、しかも嫌気性状態を適宜保ちな
がら、前記有機性廃棄物に自然に付着する微生物を作用
させているので、乳酸菌の活動に適した条件となり、高
濃度でしかも高選択的に乳酸を生成させることができ
る。請求項5記載の有機性廃棄物からの乳酸の製造方法
は、前記微生物に、乳酸菌及び/又は乳酸菌の活動を助
ける菌を加えるので、自然に付着、混在している菌が少
ない有機性廃棄物でも、高濃度でしかも高選択的に乳酸
が生成できる。請求項6記載の有機性廃棄物からの乳酸
の製造方法は、前記微生物に加えて、乳酸醗酵させた汚
泥滓及び/又は余剰汚泥を投入する、自然に付着、混在
している菌が少ない有機性廃棄物でも、高濃度でしかも
高選択的に乳酸が生成できる。According to the method for producing lactic acid from organic waste according to any one of claims 1 to 6, organic waste comprising garbage, waste from food factories, unused organic resources, or excess sludge. By adding a suitable amount of sugar and water to the product and allowing the microorganism to act, it has become possible to produce highly useful lactic acid at a high concentration and with high selectivity. From the lactic acid thus obtained, a biodegradable plastic can be produced chemically or by a fermentation method, lactic acid itself can be used as a food additive, and after a chemical treatment, It can also be used as a raw material for various chemical products. Furthermore, while containing various effective resources, it also played a major role in the treatment of organic waste that had been treated mainly by incineration, landfill, and ocean dumping. In particular, in the method for producing lactic acid from organic waste according to claim 2, microorganisms are added to organic waste by adding a suitable amount of saccharide and water, and further controlling the temperature, pH, and dissolved oxygen concentration. Since it is allowed to act, the conditions are suitable for the activity of lactic acid bacteria, and lactic acid can be produced with high concentration and high selectivity. In the method for producing lactic acid from organic waste according to the third aspect, since the microorganisms include lactic acid bacteria or bacteria that assist the activity of lactic acid bacteria and lactic acid bacteria, lactic acid can be produced with high concentration and high selectivity. The method for producing lactic acid from organic waste according to claim 4 comprises one or more of garbage, waste from food factories, unused organic resources, or excess sludge, and contains nitrogen. To the organic waste A, saccharide B such as glucose, sucrose, starch and water C are added at the following ratio, and the temperature is kept from normal temperature to 55 ° C. Since the microorganisms that naturally adhere to the organic waste are allowed to act while appropriately maintaining the anaerobic state below, conditions suitable for the activity of lactic acid bacteria are obtained, and lactic acid is produced at a high concentration and selectively. Can be done. The method for producing lactic acid from organic waste according to claim 5, wherein lactic acid bacteria and / or bacteria that assist the activity of the lactic acid bacteria are added to the microorganisms, so that organic wastes with few naturally attached and mixed bacteria are added. However, lactic acid can be produced at a high concentration and with high selectivity. 7. The method for producing lactic acid from organic waste according to claim 6, wherein in addition to the microorganism, sludge and / or surplus sludge fermented with lactic acid is added, and the organic matter with a small amount of bacteria attached and mixed naturally. Lactic acid can be produced at a high concentration and with high selectivity even for toxic waste.
Claims (6)
用有機性資源、若しくは余剰汚泥等の1又は2以上から
なって窒素分を含む有機性廃棄物に、適度の糖質と水を
加え、微生物を作用させて、高濃度、高選択的に乳酸を
生成させることを特徴とする有機性廃棄物からの乳酸の
製造方法。An organic waste containing one or two or more of raw garbage, waste from a food factory, unused organic resources, or excess sludge, and containing nitrogen content, containing a suitable amount of sugar and water. A method for producing lactic acid from organic waste, characterized in that lactic acid is produced in a high concentration and with high selectivity by adding microorganisms to the microorganisms.
用有機性資源、若しくは余剰汚泥等の1又は2以上から
なって窒素分を含む有機性廃棄物に、適度の糖質と水を
加え、更に、温度、pH、溶存酸素濃度を制御した状態
で、微生物を作用させて、高濃度、高選択的に乳酸を生
成させることを特徴とする有機性廃棄物からの乳酸の製
造方法。2. An organic waste containing one or two or more of raw garbage, waste from a food factory, unused organic resources, or excess sludge, and containing nitrogen, and a suitable amount of sugar and water. A method of producing lactic acid from organic waste, characterized in that lactic acid is produced at a high concentration and with high selectivity by allowing microorganisms to act under a controlled temperature, pH and dissolved oxygen concentration. .
乳酸菌の活動を助ける菌を含む請求項1又は2記載の有
機性廃棄物からの乳酸の製造方法。3. The method for producing lactic acid from organic waste according to claim 1, wherein the microorganism includes a lactic acid bacterium or a bacterium that assists the activity of the lactic acid bacterium and the lactic acid bacterium.
用有機性資源、若しくは余剰汚泥等の1又は2以上から
なって窒素分を含む有機性廃棄物Aに対して、グルコー
ス、シュークロース、デンプン等の糖質Bと、水Cとを
以下の重量割合で添加し、常温から55℃の温度を保つ
と共に、pHを3を超えて12以下で、しかも嫌気性状
態を適宜保ちながら、前記有機性廃棄物に自然に付着す
る微生物を作用させて、高濃度でしかも高選択的に乳酸
を生成させることを特徴とする有機性廃棄物からの乳酸
の製造方法。 A=(1〜10)×B C=(0.3〜5)×(A+B)4. An organic waste A containing one or two or more of raw garbage, waste from a food factory, unused organic resources, or excess sludge, and containing nitrogen, glucose, shoe, etc. Sugar B such as sucrose, starch and the like and water C are added in the following weight ratios, and the temperature is kept from normal temperature to 55 ° C., and the pH is more than 3 and is 12 or less, and the anaerobic state is appropriately maintained. A method for producing lactic acid from organic waste, characterized in that lactic acid is produced at a high concentration and with high selectivity by causing microorganisms that naturally adhere to the organic waste to act. A = (1-10) × B C = (0.3-5) × (A + B)
の活動を助ける菌を加えることを特徴とする請求項4記
載の有機性廃棄物からの乳酸の製造方法。5. The method for producing lactic acid from organic waste according to claim 4, wherein lactic acid bacteria and / or bacteria that help the activity of the lactic acid bacteria are added to the microorganism.
泥滓及び/又は余剰汚泥を投入する請求項1〜5のいず
れか1項に記載の有機性廃棄物からの乳酸の製造方法。6. The method for producing lactic acid from organic waste according to claim 1, wherein sludge and / or excess sludge fermented with lactic acid is added to the microorganisms.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP9148599A JPH10313887A (en) | 1997-05-21 | 1997-05-21 | Production of lactic acid from organic waste |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP9148599A JPH10313887A (en) | 1997-05-21 | 1997-05-21 | Production of lactic acid from organic waste |
Publications (1)
Publication Number | Publication Date |
---|---|
JPH10313887A true JPH10313887A (en) | 1998-12-02 |
Family
ID=15456374
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP9148599A Pending JPH10313887A (en) | 1997-05-21 | 1997-05-21 | Production of lactic acid from organic waste |
Country Status (1)
Country | Link |
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JP (1) | JPH10313887A (en) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2003061695A (en) * | 2001-08-24 | 2003-03-04 | Kuraudo:Kk | Method for producing useful material from garbage or the like |
CN103420489A (en) * | 2013-07-09 | 2013-12-04 | 湖南工业大学 | Novel method for producing organic acid by using industrial wastewater produced in food fermentation process |
-
1997
- 1997-05-21 JP JP9148599A patent/JPH10313887A/en active Pending
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2003061695A (en) * | 2001-08-24 | 2003-03-04 | Kuraudo:Kk | Method for producing useful material from garbage or the like |
CN103420489A (en) * | 2013-07-09 | 2013-12-04 | 湖南工业大学 | Novel method for producing organic acid by using industrial wastewater produced in food fermentation process |
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