JPH10313822A - Intestinal bacterial flora improver and composition containing the same - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain the subject improver composed of primeverose for improving intestinal bacterial flora by accelerating growth of the so-called benign bacteria, e.g. Bifidobacterium. SOLUTION: This improver is composed of primeverose, showing functions of accelerating growth of Bifidobacterium and improving intestinal bacterial flora. A tablet type health food can be produced by, e.g. tabletting a mixture of 20 pts.wt. of primeverose, 40 pts.wt. of lactose, 1 pt.wt of calcium lactate, 1 pt.wt. of zinc stearate, 10 pts.wt. of freeze-dried Bifidobacterium, 8 pts.wt. of hydroxypropylmethyl cellulose and 20 pts.wt. of cellulose.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

TECHNICAL FIELD The present invention relates to a food composition suitable for improving intestinal flora.

[0002]

BACKGROUND OF THE INVENTION Primeverose is a glucopyranose 6
Is a disaccharide in which the 1-position of xylose and the 1-position of xylose are linked by an ether bond.
It is known that there exists a glycoside in which an aglycone such as terpene is bonded at the position. Primeverose itself is known for its existence, but much is unknown about the properties and actions it possesses.
In particular, it is completely unknown that this substance is beneficial for improving the intestinal flora. In addition, since this is not contained in food materials that are usually eaten, it is not known or used to use it as a food material, nor to eat foods containing it. .

[0003] On the other hand, the bacterial composition of the intestinal flora greatly affects diarrhea, constipation and infectious diseases, and alters the bacterial composition of the flora, for example, to grow favorable bacteria such as bifidobacteria. Has already been shown from clinical studies to be very beneficial for the prevention and amelioration of symptoms. As means for improving such intestinal flora, N-acetylglucosamine, pantethines, certain peptides, nucleic acid-related substances, saccharides such as lactulose that cannot be digested by stomach acid, and the like are administered to grow Bifidobacteria. Methods for promoting are already known. However, these substances were not in a state where a satisfactory effect such as limitation of the amount of use due to the strength of action or off-flavor or the like could be necessarily exerted. That is, at present, the appearance of a new means for improving the intestinal flora has been awaited.

[0004]

SUMMARY OF THE INVENTION The present invention has been made under such circumstances, and it is an object of the present invention to provide means for improving the intestinal flora.

[0005]

In view of such a situation, the present inventors
As a result of intensive studies in search of means for improving the intestinal flora, it was found that primeverose has such an effect. Furthermore, by ingesting a food composition containing primeverose, in the intestinal flora, so-called good bacteria such as bifidobacteria are promoted to grow, and it has been found that the intestinal flora is improved, thereby completing the invention. . Less than,
The present invention will be described in more detail mainly on the embodiments of the present invention.

[0006]

BEST MODE FOR CARRYING OUT THE INVENTION

(1) Intestinal flora improving agent of the present invention The intestinal flora improving agent of the present invention comprises primeverose.
Primeverose is a disaccharide in which the 6th position of glucopyranose and the 1st position of xylose are linked by an ether bond, and in nature, there is a glycoside in which an aglycone such as terpene is linked to the 1st position of primeverose. Are known. Primeverose itself is known for its existence, but much is unknown about the properties and actions it possesses. In chemical synthesis, for example, acetylglucopyranose and α
-It can be obtained by reacting acetobromoxylose with silver nitrate or the like as a catalyst. In the case of enzyme synthesis, it can be obtained by condensing D-glucose and xylobiose in the presence of an enzyme such as pectinase. Among these production methods, preferred is an enzyme synthesis method having excellent stereoselectivity. Hereinafter, examples of the enzyme synthesis method will be described.

(Production Example) Xylobiose and glucose in a 100 mM citrate phosphate buffer (pH 4.0) at a molar ratio of 1: 2 in the presence of pectinase (1.5 U / ml)
After reacting at 40 ° C. for 24 hours, the reaction was stopped by heating for 5 minutes. The reaction solution was purified by column chromatography using activated carbon-celite as a carrier equilibrated with water (elution solvent; water: ethanol 100: 0 → 70: 30), and then recrystallized from ethanol to obtain 42 mg of xylobiose.
Permeverose was obtained in a yield of 6.35%.

[0008] The thus obtained premeverose increases the number of so-called good bacteria such as bifidobacteria in the intestinal flora and regulates the intestinal flora, as shown in Examples below.
It exerts an excellent effect on the prevention and treatment of diarrhea, constipation, infectious diseases, etc. caused by disturbance of the intestinal flora. This is because primeverose is stable against digestive juices such as stomach acid, and most of it reaches the large intestine, becomes a nutrient medium for beneficial bacteria such as bifidobacteria, promotes the production of acetic acid and lactic acid, etc. It is possible to suppress the growth of bacteria and spoilage bacteria that cause spontaneous infectious diseases and the like by lowering them, and suppress the generation of ammonia, indole, cresol, etc. produced by these harmful bacteria.

(2) Food composition of the present invention The food composition of the present invention is characterized by containing an intestinal flora improving agent comprising the above-mentioned primeverose. The preferred content of primeverose in the food composition of the present invention may slightly vary depending on the type of food, but is preferably 0.1%.
0.01 to 20% by weight, more preferably 0.05 to 1% by weight.
It is 5% by weight, more preferably 0.1 to 10% by weight. The food composition of the present invention can be applied without any particular limitation as long as it is in the form of food, for example, sweets such as candy, chocolate, yokan, willow, cake, cookies, noodles Staple foods such as bread, pizza, etc., dairy products such as yogurt and cheese, processed meat products such as sausages and hams, processed fish products such as kamaboko, bamboo rings, and starch, retorts or canned curries, white stews, beef stews, etc. Foods and the like can be exemplified. The food composition of the present invention may contain optional components used in these food compositions, in addition to premeverose, which is an essential component. Optional ingredients include main ingredients such as grains, meats, vegetables, and fish, as well as flavoring agents, preservatives, amino acids, sweeteners, spices, preservatives, antioxidants, freshness preservatives, coloring agents, and coloring. Agents, sour agents, bitter agents, emulsifiers, thickeners, stabilizers, dispersants and the like.
Further, it is possible to contain a substance having an effect of improving the intestinal flora. Examples of such substances include N-acetylglucosamine and pantethines, certain peptides, nucleic acid-related substances, and sugars such as lactulose that are not digested by gastric acid. Bacteria such as Bifidobacterium and Bulgarian yogurt can also be added.
In addition, calcium, magnesium,
Since the food composition of the present invention also has an action of promoting the absorption of trace elements such as iron, manganese, zinc, copper, potassium and phosphorus, the food composition of the present invention also has an action of preventing and improving these deficiencies. Furthermore, since premeverose has no caries effect, there is little worry about caries. Also, because it is difficult to digest, the amount of feces also increases,
Constipation can be improved and prevented in combination with the improvement of the bacterial flora. It can be suitably used not only for humans but also for livestock and poultry.

[0010]

The present invention will be described in more detail with reference to the following examples, but it goes without saying that the present invention is not limited to these examples.

<Example 1> The digestibility of primeverose was determined by the method of Okada et al. (Journal of Japan Society of Nutrition and Food Science, No. 43).
Vol. 1, No. 23, in vitro).
Maltose, trehalose, and isomaltose were used as comparative examples. The results are shown in Table 1 in terms of the decomposition rate (producing ratio of glucose to total sugars). From these results, it can be seen that primeverose is stable in various digestive fluids and reaches the large intestine. In addition, since it is difficult to digest, it can be called a low calorie sugar. That is, it turns out that the food composition of the present invention is also suitable as a low-calorie food.

[0012]

[Table 1]

Example 2 The assimilation of primeverose with respect to good intestinal bacteria such as bifidobacteria and bad intestinal bacteria was examined. That is, Tomohiko Mitsuoka, published by Sobunbunsha, “World of Intestinal Bacteria (Separation and Identification of Anaerobic Bacteria)”, p. 325 (1984)
Year), the PYF medium and sugar were added at 0.5 w / v.
Cultured at 37 ° C. for 96 hours in a sweetened PYF medium containing
The culture solution was diluted 5-fold, and the growth of intestinal bacteria was determined from the turbidity. As comparative examples, glucose, trehalose, and isomaltose were used. Turbidity is less than 0.1, minus 0.1.
1 to less than 1.5 ±, 1.5 to less than 0.2 +,
0.2 or more and less than 0.3 were determined as ++, and 0.3 or more was determined as +++. Table 2 shows the results. From this table, it can be seen that Primeverose selectively assimilates only to good intestinal bacteria such as Bifidobacterium (Bifidobacterium) and Lactobacillus (Lactobacillus) and promotes their growth in the intestine. You can see that. That is, in consideration of the results of Example 1, it can be understood that oral administration of primeverose can improve intestinal bacterial flora.

[0014]

[Table 2]

Example 3 Changes in the amount of feces caused by administration of glucose, isomaltose, trehalose, and primeverose were examined using mice that had been bred in advance with a concentrated feed. That is, ICR mice (male, 35 to 40 g) reared for 4 weeks on a feed obtained by kneading and mixing 50 parts by weight of a solid feed, 10 parts by weight of soybean protein and 10 parts by weight of lard
Ten animals per group were bred in a metabolic cage for 3 days on a feed obtained by kneading and forming 70 parts by weight of a crushed solid feed and 30 parts by weight of a saccharide, and the total amount of feces for 3 days was measured. The control group received only chow. The result was expressed as the rate of increase in fecal volume relative to the control group [(fecal volume of administration group-fecal volume of control group) / fecal volume of control group x 1
00] is shown in Table 3. From this table, it can be seen that the amount of feces in the premeverose-administered group is significantly increased, and that the premeverose is effective in improving constipation and the like.

[0016]

[Table 3]

Example 4 A tablet-shaped health food was prepared according to the following formulation. That is, the powder components were mixed well and tableted with a tableting machine to obtain a tablet of 200 mg. People suffering from constipation 5
When they took this tablet two times each in the morning, afternoon, and evening for one month, constipation improved, and three of them began to have regular bowel movements once a day. Primeverose 20 parts by weight Lactose 40 parts by weight Calcium lactate 1 part by weight Zinc stearate 1 part by weight Bifidobacterium lyophilizate 10 parts by weight Hydroxypropyl methylcellulose 8 parts by weight Cellulose 20 parts by weight

Example 5 A juice was prepared according to the following recipe. That is, the prescription components were mixed well, sterilized, filled aseptically into a bottle, and sealed to obtain a juice. Valencia orange juice 50 parts by weight Carrot juice 30 parts by weight Primeverose 10 parts by weight Vitamin C 0.1 part by weight Vitamin B6 hydrochloride 0.1 part by weight Isomaltose 9.8 parts by weight

Example 6 A candy was prepared according to the following recipe. That is, the formulation components are heated and dissolved,
The mixture was poured into a mold, cooled and solidified, and removed from the mold to obtain a candy. Starch syrup 40 parts by weight primeverose 5 parts by weight citric acid 0.1 parts by weight sucrose 54.9 parts by weight

<Example 7> Bread was prepared in the following manner. That is, the ingredients are kneaded well at 40 ° C.
Primary fermentation for 0 minutes, molding after degassing,
Secondary fermentation for 5 minutes, bake in oven at 200 ° C for 30 minutes,
Cooling to room temperature gave a bread. Strong flour 500 parts by weight Salt 10 parts by weight Dry yeast 10 parts by weight Primeverose 15 parts by weight Butter 30 parts by weight Milk 210 parts by weight

Example 8 Kamaboko was obtained according to the following formulation. That is, the ingredients were kneaded well, coated and molded on a plate, and steamed with steam to obtain Kamaboko. Alaska pollack ground meat 89.9 parts by weight Primeverose 10 parts by weight Vitamin C 0.1 part by weight

[0022]

According to the present invention, means for improving the intestinal flora can be provided.

 ──────────────────────────────────────────────────続 き Continued on the front page (72) Inventor Kanzo Sakata 836 Otani, Shizuoka City, Shizuoka Prefecture Inside the Faculty of Agriculture, Shizuoka University (72) Inventor Yasushi Usui 836 Otani, Shizuoka City, Shizuoka Prefecture Inside the Faculty of Agriculture, Shizuoka University (72) Inventor Shuji Watanabe 836 Otani, Shizuoka City, Shizuoka Prefecture Shizuoka University Faculty of Agriculture

Claims (5)

[Claims] 1. An intestinal flora improving agent comprising primeverose. 2. The intestinal flora-improving agent according to claim 1, wherein the improvement of the intestinal flora is an action of promoting the growth of bifidobacteria. 3. A food composition comprising the intestinal flora improving agent according to claim 1 or 2. 4. A food composition containing primeverose. 5. The food composition according to claim 4, which has an intestinal flora improving effect.