JPH10279461A - Composition - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain a composition, capable of improving effects essentially possessed by an active oxygen scavenger and effectively suppressing the production of peroxylipids and extremely useful for the beauty culture and medicine by using the active oxygen scavenger with a specific extract in combination. SOLUTION: This composition is obtained by formulating an active oxygen scavenger with a lignan glycoside in combination. A sesaminol glycoside represented by formula I [R1 and R2 are each glycosyl composed of one to four saccharides, which are one or more selected from glucose, galactose and fructose; (a) to (c) are each 0 or 1], especially a compound represented by formula II (Glc is the glycosyl) or formula III or IV is preferred. The amount of the formulated sesaminol glycoside is 0.001-5 wt.% in the case of using, e.g. the compound represented by formula 1 to IV. A superoxide dismutase, mannitol, hydroquinone, etc., are preferred as the active oxygen scavenger. The amount thereof formulated is 0.0001-5 wt.%.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

[0001] The present invention relates to a composition, and more particularly, to the production of a lipid peroxide resulting from the production of active oxygen in the skin by combining a lignan glycoside with an active oxygen scavenger. The present invention also relates to compositions such as cosmetics and external medicines, which have excellent anti-aging effects and skin roughness improving effects capable of preventing skin inflammation, blackening, aging and the like.

[0002]

2. Description of the Related Art Conventionally, medicinal ingredients have been added to external preparations such as emulsions, creams, lotions, packs, detergents, foundations, ointments, dispersions and the like for the purpose of imparting a prescribed medicinal effect to them. ing. In recent years, as one of the causes of skin aging, the adverse effect of active oxygen on the living body has been cited. Active oxygen includes singlet oxygen, superoxide, hydroxyl radical, hydrogen peroxide, etc., which are known to damage lipids, nuclei, proteins, and enzymes. If it occurs, it may cause illness or death. In addition, in terms of quantity, at least the accumulation of damage caused by active oxygen lowers the biological function, which is a cause of the so-called aging phenomenon. For this reason, various active oxygen removers and antioxidants have been conventionally developed.

[0003]

Therefore, there is a demand for the development of a highly effective active oxygen scavenger for the purpose of protecting the living body and preventing aging. In addition, it must be sufficiently satisfactory from the viewpoint of safety. That is, there has been a demand for the development of a medicinal component having an excellent active oxygen removal and / or antioxidant effect and suitable for application to biological systems.

[0004]

Means for Solving the Problems The present inventors have conducted intensive studies to improve the effect of the medicinal component of the external preparation for skin, and as a result, when the lignan glycoside extract and the active oxygen scavenger are combined, The present inventors have found that the action of the active oxygen removing agent is sufficiently exhibited, and completed the present invention.

That is, the present invention relates to the following components (A) and (B): (A) a lignan glycoside represented by the structural formula (I): (B) an active oxygen scavenger

Embedded image And lignan glycosides represented by the following structural formulas (II-a) and (II-b):
Or, those containing one or more of the sesaminol glycosides represented by (II-c) as a main component are preferable, and in a preferred embodiment, the lignan glycoside is a sesame seed or a humidified product thereof. It is a hydrated lower alcohol extract of germinated bodies or their defatted products.

Embedded image

Embedded image

Embedded image

[0006]

BEST MODE FOR CARRYING OUT THE INVENTION The lignan glycoside, which is the component (A) of the present invention, is represented by the above general formula (I). That is, the lignan glycoside according to the present invention comprises 2
The aglycone portion has a methylenedioxyphenyl group and a sugar portion in which one to four sugar residues of glucose, galactose or fructose are bonded to the hydroxyl group.

[0007] The lignan glycoside is preferably glucoside lignan in which the sugar residue in the structural formula (I) is a diglucoside residue and / or a triglucoside residue, and still more preferably the structural formula (II) −
One, two or more of the compounds represented by a), (II-b) or (II-c), or those containing these as main components. Further, the method of preparing the same is not particularly limited.
No. 145066).

[0008] The content of lignan glycosides in the composition of the present invention cannot be uniformly defined by the difference in the content of each component of the lignan glycoside.
In the case of the aqueous lower alcohol extract prepared by the method disclosed in No. 66, the content is 0.05 to 20% by weight (hereinafter simply referred to as “%”), preferably 0.1 to 10%,
0.01 to 10% when blending crude lignan glycoside,
It is preferably 0.05 to 5%, and a highly purified product (of the lignan glycoside represented by the structural formula (I), (II-a), (II-b) or (II-c)) When at least one of them is contained in a total amount of about 60% or more), 0.001 to 5%, preferably 0.01 to 1%.
%.

On the other hand, the active oxygen scavenger as the component (B) of the present invention includes the following.

[0010] Superoxide dismutase (hereinafter referred to as S
OD), mannitol, carotenoids such as beta-carotene, hydroquinone, bilirubin, tryptophan, histidine, quercetin, quercitrin,
Catechin and its derivatives, gallic acid and its derivatives, plant extracts containing flavonoids such as gougon extract and ginkgo extract in its components, saxifrage extract, gokahi extract, yasajitsu extract and dicopi extract, and the like, One or more of these may be used in combination.

Among these active oxygen scavengers, particularly preferred are carotenoids such as SOD, mannitol, beta-carotene, hydroquinone, catechin and derivatives thereof, pentagon extract, ginkgo extract, saxifrage extract, and kokahi extract. , Jasazi extract and dickopi extract. Each of these components is not particularly limited in its origin, and is derived from animals, microorganisms,
Any of synthetic products may be used. Also, the extraction method,
The production method such as the purification treatment method is not particularly limited.

The amount of the component (B) in the composition of the present invention is not particularly limited, but is preferably 0.0001 to 5% as an active oxygen remover, more preferably
0.001 to 3%. Within this range, excellent effects of preventing skin aging and improving skin roughness can be obtained. That is,
It is effective in preventing the formation of lipid peroxide due to the generation of active oxygen in the skin and the prevention of skin inflammation, blackening, aging and the like. These active oxygen removers can be used alone or in combination of two or more.

The composition of the present invention can be prepared by blending the essential components (A) and (B) with various types of bases known as ordinary skin external preparations according to a conventional method. it can.

The form of the external preparation is not particularly limited.
For example, skin care cosmetics such as milky lotions, creams, lotions, packs, cleaning agents, makeup cosmetics such as lipsticks and foundations, cosmetics for the scalp and hair, and pharmaceuticals such as ointments, dispersions, creams, and external solutions And so on.

In the composition of the present invention, in addition to the above-mentioned essential components, components to be blended with ordinary external preparations, for example, oils, powders, surfactants, purified water, lower alcohols, polymer compounds, gels An agent, an ultraviolet absorber, an ultraviolet scattering agent, an antioxidant, a dye, a preservative, a fragrance, a cosmetic ingredient, and the like can be appropriately selected and used within a range not to impair the effects of the present invention.

[0016]

EXAMPLES Next, the present invention will be described in more detail with reference to Reference Examples, Test Examples and Examples, but the present invention is not limited thereto.

Reference Example 1 Production of aqueous methanol extract Pre-sterilized quartz sand was spread on a stainless steel vat of 300 cm ² , and 10 g of Chinese sesame seeds were sprinkled thereon. And germinated for 2 days in a constant temperature bath. The germination rate was 89% or more. A certain amount of germinated material having a similar germination state was ground with a blender together with 100 ml of hydrated methanol (80% (v / v)). The residue was filtered, and the filtrate was concentrated to dryness to obtain a methanol extract. Then, the extract was washed by extraction with n-hexane to remove fat-soluble substances, thereby obtaining a water-containing methanol extract. The aqueous methanol extract was redissolved in 100 ml of aqueous methanol (80% (v / v)) and subjected to high performance liquid chromatography (HPLC) to analyze the composition.

The HPLC conditions were as follows: a pump (CCPM, manufactured by Tosoh Corporation) was used and a column (Soken Pak ODS-W5) was used.
μ, 10 mmΦ × 250 mm), UV absorption detector (U
V-8000, manufactured by Tosoh Corporation), elution was performed using a linear gradient of water: methanol starting at 90:10 (v: v) and 60:60 minutes later at 10:90 (v: v). The flow rate was 1 ml / min, and the detection wavelength was 280 nm.

As a result of HPLC analysis, the composition and content of lignan glycosides in the aqueous methanol extract were determined using sesamin as an external standard, and sesaminol triglycoside (structural formula (II-a)), sesaminol diglycoside (Structural Formula (II-b)) and Sesaminol monoglycoside (Structural Formula (II-c)), which are present in a hydrous methanol extract in an amount of 80 mg. %, Sesaminol diglycoside 50%, sesaminol monoglycoside 10%
Met.

The chemical structure of each lignan glycoside component was confirmed by the following method using each purified product purified to a single component by preparative HPLC under the same conditions as described above. That is, 1N hydrochloric acid was added to each purified product, hydrolyzed at 100 ° C. for 30 minutes, extracted with ethyl acetate, and separated into an ethyl acetate layer and an aqueous layer. The ethyl acetate layer was concentrated to dryness at 40 ° C. or lower, subjected to trimethylsilylation treatment with TMS-PZ (manufactured by Tokyo Chemical Industry Co., Ltd.), and subjected to gas chromatography (GLC) to quantitatively analyze lignan (external standard: sesamin).

The GLC conditions are as follows. GLC apparatus: Hewlett-Packard 5890, column: D
B-17HT (15mx0.319mm, film t
kickness: 0.15 μm, J & W SCIEN
TIFIC), injection method: split method (split ratio 1/10), column temperature: 270 ° C., carrier gas:
helium.

The aqueous layer was subjected to a pretreatment filter for HPLC (pore size: 0.2 μm, Meishoridisk W-13).
2, Tosoh Corporation), add 5 ml of acetone to the filtrate, concentrate under reduced pressure to dryness, and then TMS-PZ (same as above)
And subjected to GLC for quantitative analysis of sugars (external standard: glucose, galactose,
Fructose).

The GLC conditions are as follows: Column: DB-170
1 (15m x 0.25mm, film thickne
ss: 1.0 μm, manufactured by J & W SCIENTIFIC) Injection method: split method (split ratio 1/5)
0), except that the column temperature was set to 180 ° C.

Reference Example 2 Production of Crude Lignan Glycoside A hydrous methanol extract obtained in the same manner as in Reference Example 1 was dispersed in 100 ml of water, and the same volume of n-hexane was added and shaken vigorously. This extraction operation was repeated three times to remove fat-soluble substances. To the residual solution from which the n-hexane layer was completely removed, the same volume of n-butanol which had been saturated with water in advance was added, and the mixture was vigorously shaken. This extraction operation was repeated twice to remove water-soluble substances. After washing the n-butanol layer twice with the same volume of water,
It was concentrated to dryness under reduced pressure to obtain a crude lignan glycoside.

According to HPLC analysis by the method described in Reference Example 1, the lignan glycosides in the crude lignan glycoside include sesaminol triglycoside, sesaminol diglycoside and sesaminol monoglycoside, which are crude lignan glycosides. The saccharide contained 70 mg, and its composition was 45% sesaminol triglycoside, 48% sesaminol diglycoside, and 7% sesaminol monoglycoside.

Reference Example 3 Production of Highly Purified Lignan Glycoside The crude lignan glycoside obtained by the method described in Reference Example 2 was
The resultant was subjected to partition chromatography using ODS as a carrier.
YMC-GEL ODS-A (Yamamura Chemical Co., Ltd.) 60
g was packed in a glass column having a diameter of 3 cm and a length of 50 cm, and equilibrated by flowing water. To this, 1 g of the crude lignan glycoside was loaded on the top of the column. The fraction components were eluted by a step elution method in which the methanol concentration was sequentially increased from water. Fractions eluted with 30-60% (v / v) methanol were collected and concentrated under reduced pressure to obtain about 100 mg of a column fraction.

This was repeatedly subjected to preparative HPLC, and purification was performed until each lignan glycoside component became single.
As a result, 5 to 10 mg of each purified lignan glycoside of sesaminol triglycoside, sesaminol diglycoside and sesaminol monoglycoside was obtained. The content of all these lignan glycoside components was 2.0% (wt / wt) per dried germination product and 4.5% (wt / wt) per hydrated methanol extract.

REFERENCE EXAMPLE 4 Production of Orgon Extract A 10 g of Orgon (Scarabae root) was cut into small pieces, 100 ml of absolute ethanol was added, the mixture was extracted at room temperature for 5 days with occasional stirring, and filtered to obtain an Orgon extract.

Reference Example 5 Production of Gokahi Extract 10 g of whole Gokahi plant was cut into small pieces and the water content was 50% (v /
v) 100 ml of ethanol was added, and the mixture was extracted at room temperature for 3 days with occasional stirring, and filtered to obtain a mosquito extract.

Reference Example 6 Production of Saxifraga extract 25 g of Saxifraga whole plant was finely cut and 30% (v / v)
300 ml of 1,3-butylene glycol was added, extracted at room temperature for 3 days with occasional stirring, and filtered to obtain a saxifrage extract.

Test Example 1 Test for measuring superoxide removal activity The lignan glycoside and the active oxygen remover listed in Table 1 were used alone or in combination as a sample, diluted with water, and then subjected to the following measurement method to determine the superoxide removal activity. Was measured.

(Measurement method) A substrate solution [3.0] was added to 2.4 ml of a 0.05 M sodium carbonate buffer (pH 10.2).
mM xanthine (dissolved in 0.05 M sodium carbonate buffer)] 0.1 ml, 3.0 mM EDTA 0.1
ml, 0.15% (w / v) bovine serum albumin
1 ml, 0.75 mM nitro blue tetrazolium
0.1 ml and 0.1 ml of each test sample were mixed at 25 ° C.
For 10 minutes. Then, an enzyme solution [xanthine oxidase solution (about 0.04 units / m in purified water)
The reaction was started by adding 0.1 ml and the reaction was started at 25 ° C.
After incubation for 20 minutes in 6 mM CaCl 2
The reaction is stopped by adding 0.1 ml. Then 560 nm
The absorbance (A) at is measured.

As a control, the absorbance (B) of a sample to which purified water was added instead of the test sample, and 6 blanks for each sample.
After stopping the reaction by adding 20.1 ml of mM CaCl, the absorbance (C) of the sample to which 0.1 ml of xanthine oxidase was added was measured, and the superoxide removal rate was calculated from the following equation. Table 1 shows the results.

[0034]

(Equation 1) A: Absorbance of sample by enzyme reaction B: Absorbance of control by enzyme reaction C: Absorbance of sample by non-enzyme reaction

[0035]

[Table 1] * 1: Manufactured in Reference Example 3 * 2: Sigma; obtained from bovine erythrocytes (3.570 units / mg) * 3: Manufactured in Reference Example 4

As is clear from the results shown in Table 1, the lignan glycoside alone had SOD-like activity,
In addition, the use of the extract in combination with the pentagon extract showed a synergistic effect and was extremely effective in removing active oxygen.

Example 1 Cream: A cream was prepared by the composition shown in Table 2 and by the following production method, and its beautiful skin effect and skin aging prevention effect were examined. The results are also shown in Table 2.

[0038]

[Table 2] * 1: Manufactured in Reference Example 1 * 2: Manufactured in Reference Example 5 * 3: Wako Pure Chemical Industries

(Production method) Components (1)-(7), (11) and (12) are mixed and heated to 70 ° C. B. Mix components (8) and (13) and heat to 70 ° C
To keep. C. After B was added to A and mixed, the mixture was cooled, (9) and (10) were added, and the mixture was uniformly mixed to obtain a cream.

(Test method) 25 per test cream
A panel consisting of 15 women aged 55 to 55
After washing the face for 12 weeks, an appropriate amount of the test cream was applied to the face. The skin and skin aging prevention effects of the application were evaluated according to the following criteria.

(Evaluation criteria) Beautifying effect: <Evaluation> <Contents> Effective The dullness of the skin became less noticeable. Slightly effective The dullness of the skin became less noticeable. Ineffective No change from before use.

Anti-aging effect of skin: <Evaluation> <Contents> Effective Skin laxity and gloss were improved. Slightly effective Skin glue, gloss slightly improved. Ineffective No change from before use.

As shown in Table 2, the products of the present invention 1 and 2
By applying to the skin a cream that contains a lignan glycoside extract and an active oxygen remover represented by, the prevention of the occurrence of "dullness", etc., and beautiful skin,
It has been revealed that skin abrasion and gloss are improved and that skin aging is prevented.

Example 2 Lotion: A lotion was prepared according to the following formulation and the following method. (Prescription) (%) (1) Glycerin 6.0 (2) 1,3-butylene glycol 5.5 (3) Polyoxyethylene (20EO) sorbitan 1.5 monolaurate (4) Ethanol 9. 0 (5) Hydrous methanol extract * 1 10.0 (6) Mannitol * 2 0.5 (7) Preservative appropriate amount (8) Perfume appropriate amount (9) Purified water balance * 1 Produced in Reference Example 1 * 2 Kanto Chemical Co., Ltd.

(Production method) Components (3), (4), (7) and (8) are mixed and dissolved. B. Components (1), (2), (5), (6) and (9) are mixed and dissolved. C. A and B were mixed and made uniform to obtain a lotion.

Example 3 Emulsion: An emulsion was prepared according to the following formulation and the following production method. (Formulation) (%) (1) Polyoxyethylene (10EO) sorbitan 1.0 monostearate (2) Polyoxyethylene (60EO) sorbitan 0.5 tetraoleate (3) Glyceryl monostea Rate 1.0 (4) Stearic acid 0.5 (5) Behenyl alcohol 0.5 (6) Squalane 8.0 (7) Crude lignan glycoside * 1 3.0 (8) Ergocalciferol * 2 0.02 (9) Ogre extract * 3 1.0 (10) Preservative 0.1 (11) Carboxyvinyl polymer 0.1 (12) Sodium hydroxide 0.05 (13) Ethanol 5.0 (14) Purified water residue Amount (15) Appropriate amount of fragrance * 1 Manufactured in Reference Example 2 * 2 Sigma * 3 Manufactured in Reference Example 4

(Production method) The components (9) to (14) are mixed by heating and kept at 70 ° C. B. The components (1) to (6) are mixed by heating and kept at 70 ° C. C. Add A to B, mix and emulsify uniformly. D. After cooling C, (7), (8) and (15) were added and mixed uniformly to obtain an emulsion.

Example 4 Ointment: An ointment was prepared according to the following formulation and the following production method. (Prescription) (%) (1) Stearic acid 18.0 (2) Cetanol 4.0 (3) Triethanolamine 2.0 (4) Glycerin 5.0 (5) High-purity purified lignan glycoside * 1 2.0 (6) Catechin * 2 0.5 (7) Remaining purified water * 1 Produced in Reference Example 3 * 2 Sigma

(Production method) A part of components (3), (4) and (7) are mixed by heating and kept at 75 ° C. B. Heat mix components (1) and (2) and maintain at 75 ° C. C. Add A slowly to B. D. C was dissolved in the remainder of (7) while cooling (5),
(6) was added to obtain an ointment.

The lotion of Example 2, the lotion of Example 3, and the ointment of Example 4 are all excellent in stability over time, and when applied to the skin, the skin will be improved in terms of radiance and luster, and dullness of the skin will be improved. It prevented skin aging.

Example 5 Cleaning agent: A cleaning agent was prepared according to the following formulation and the following production method. (Prescription) (%) (1) Stearic acid 10.0 (2) Palmitic acid 8.0 (3) Myristic acid 12.0 (4) Lauric acid 4.0 (5) Oleyl alcohol 1.5 (6) Purification Lanolin 1.0 (7) Appropriate amount of fragrance (8) Preservative 0.1 (9) Potassium hydroxide 6.0 (10) High-purity purified lignan glycoside * 1 0.5 (11) Japanese gogon extract * 2 0.5 (12) Remaining purified water * 1 Manufactured in Reference Example 3 * 2 Manufactured in Reference Example 4

(Production method) A. Heat and mix components (9) and (12) and maintain at 70 ° C. B. Components (1) to (6) and (8) were mixed by heating.
Keep at ° C. C. A was added to B and maintained at 70 ° C. for a while. After the saponification reaction was completed, the mixture was cooled to 50 ° C., and components (7), (10) and (11) were added, and the mixture was cooled to obtain a cleaning agent.

The cleaning agent of Example 5 has excellent stability over time,
By applying to the skin, the skin was moisturized and excellent in moisturizing properties, and the texture of the skin was adjusted, and the skin was polished and glossed.

Example 6 Pack: A pack was prepared according to the following formulation and the following production method. (Prescription) (%) (1) Polyvinyl alcohol 20.0 (2) Ethanol 20.0 (3) Glycerin 5.0 (4) Kaolin 6.0 (5) Hydrous methanol extract * 1 1.0 (6) Saxifraga extract * 2 0.2 (7) Preservative appropriate amount (8) Perfume 0.05 (9) Purified water remaining * 1 Manufactured in Reference Example 1 * 2 Manufactured in Reference Example 6

(Production method) A. Mixing the components (1), (3) to (6) and (9),
Heat to 70 ° C. and stir. B. Mix components (2), (7) and (8). C. B was added to A, mixed, and cooled to obtain a pack.

The pack of Example 6 has excellent stability over time,
By applying to the skin, the abrasion and luster of the skin were improved, and dullness of the skin and aging of the skin were prevented.

Example 7 Lip cream: A lip cream was prepared according to the following formulation and the following method. (Prescription) (%) (1) Microcrystalline wax 9.0 (2) Solid paraffin 8.0 (3) Vaseline 15.0 (4) Cetyl ethylhexanoate 15.0 (5) Liquid paraffin 8.0 (6) ) Castor oil Remaining amount (7) Purified lignan glycoside high-purity * 1 0.02 (8) β-carotene * 2 0.01 (9) Mannitol * 3 0.002 (10) Perfume appropriate amount * 1 Reference example * 2 Wako Pure Chemical Co., Ltd. * 3 Kanto Chemical Co., Ltd.

(Production method) The components (1) to (6) are heated and dissolved at 100 to 110 ° C. B. A is mixed with the components (7) to (10) at 90 ° C. C. B was cooled and molded to obtain a lip balm.

The lip balm of Example 7 was excellent in stability over time, feeling in use and finish, and when applied to the lips, kept the lips smooth and prevented roughening.

Example 8 Powder foundation: A powder foundation was prepared according to the following formulation and the following production method. (Prescription) (%) (1) Mica 50.0 (2) Titanium oxide 15.0 (3) Nylon powder 5.0 (4) Remaining kaolin (5) Color pigment 3.0 (6) Paramethoxycinnamon 2-ethylhexyl acid 2.0 (7) dimethyl polysiloxane 2.0 (8) squalane 3.0 (9) glyceryl trioctanoate 2.0 (10) petrolatum 1.0 (11) crude lignan glycoside * 10 .02 (12) tryptophan * 2 0.02 (13) perfume appropriate amount * 1 manufactured in Reference Example 2 * 2 manufactured by Kanto Chemical Co.

(Production Method) Mix components (1) to (5). B. Components obtained by heating and dissolving the components (6) to (13) are added, mixed and pulverized. C. B was filled in a metal plate and press-molded to obtain a powder foundation.

The powder foundation of Example 8 is
It was excellent in stability over time, feeling in use, and finish, and was applied to skin to suppress dullness and roughness of the skin and prevent skin aging.

[0063]

As described above, by combining the lignan glycoside with the active oxygen scavenger, the original effects of the active oxygen scavenger can be fully exhibited. That is, it has extremely high improvement and prevention effects on lipid peroxide generation, inflammation, blackening, aging, etc. due to active oxygen generation in the skin, and is therefore extremely useful in beauty and medicine.

Continued on the front page (51) Int.Cl. ⁶ Identification code FI A61K 7/00 AGZ A61K 7/00 AGZK 7/40 7/40 31/70 ADA 31/70 ADA (72) Inventor Masami Senoo Kita-ku, Tokyo 3-26-2-1004 Tabata Shinmachi (72) Inventor Kumi Kameyama 4-11-9-512 Kamiikebukuro, Toshima-ku, Tokyo

Claims (5)

[Claims] 1. A composition comprising the following components (A) and (B): (A) a lignan glycoside (B) an active oxygen scavenger. 2. A lignan glycoside having the following structural formula (I):
The composition according to claim 1, which is a sesaminol glycoside represented by the formula: Embedded image 3. A lignan glycoside having the following structural formula (II)
The composition according to claim 1 or 2, which is a lignan glycoside containing one or more of sesaminol glycosides represented by -a), (II-b) or (II-c) as a main component. . Embedded image Embedded image Embedded image 4. The lignan glycoside is a hydrated lower alcohol extract of sesame seeds, humidified or germinated bodies thereof, crushed products thereof, or defatted products thereof.
4. The composition according to 2 or 3. 5. An active oxygen scavenger comprising enzymes such as superoxide dismutase (SOD), catalase and glutathione peroxidase; carotenoids such as mannitol and beta-carotene; hydroquinone, bilirubin, tryptophan, histidine, quercetin, quercitrin, and catechin And a derivative thereof, a gallic acid and a derivative thereof, a plant extract containing flavonoids such as a gougon extract and a ginkgo extract in a component, a saxifraga extract, a gokahi extract, a yasazitsu extract and a dickopi extract, or Claim 1 which is two or more kinds.
The composition according to 2, 3 or 4.