JPH0665043A - Skin external preparation - Google Patents

Abstract

PURPOSE:To obtain a skin external preparation. having stable and excellent effects on improvement of chapped skin, prevention of aging of skin, etc., by containing extract of a rosaceous plant and a superoxide dissmutase. CONSTITUTION:The objective skin external preparation contains one or two or more kinds of substances selected from extracts of rosaceous plant such as dried flower bud of Rosa rugosa var. plena Regel, dried seed of Prunus japonica Thunb. and dried fruit of Chaenomeles lagenaria Koidz. in an amount of 0.0001-10wt.% based on dried solid content and superoxide dissmutase which is a superoxide-removing enzyme distributed in living organisms such as animals, plants, microorganisms in an amount of 0.0001-5wt.% based on dried solid content, respectively. This preparation can be formulated as lotion, cream, cosmetic water, cleansing cream, pack, cleansing agent, foundation, etc., skin external preparation for dispersion type, granular or ointment-like medicine, quasi-drug and cosmetic. The preparation has an active oxygen removing action and extremely high preventing effects on production of lipoperoxide which results from production of active oxygen in the skin due to ultraviolet rays, inflammation, blackening, aging, etc.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to an external preparation for skin, more specifically, a cosmetic having an excellent active oxygen removing action, a lipid peroxidation preventing effect, a skin aging preventing effect, a skin roughening improving effect, and the like. The present invention relates to an external preparation for skin such as pharmaceuticals.

[0002]

2. Description of the Related Art It is becoming clear that the generation of active oxygen in the skin is greatly involved in the aging of the skin. Therefore, in recent years, active oxygen production inhibitors, active oxygen scavengers, antioxidants, and the like have been increasingly applied to external skin preparations for the purpose of preventing skin aging.

On the other hand, as the medicinal components for improving skin roughness and preventing skin aging, allantoin, aloe extract, ginseng extract, placenta extract, bovine blood deproteinate, fermented metabolite and the like are known.

However, the skin external preparations containing these medicinal components cannot exert a sufficient effect for improving rough skin and preventing skin aging, and development of a skin external preparation having a more excellent cell activating effect is desired. Was there.

On the other hand, it is known that extracts of Rosaceae plants such as squid, mocca and iclinin have an anti-inflammatory effect and a whitening effect (JP-A-3-127714).
issue).

[0006]

However, when the extract of mica, mokka and iclinin (hereinafter sometimes referred to as plant extract) is blended alone, the effect of improving skin roughness and preventing aging is sufficient. It cannot be said that this is not the case, and the actual effect that the above plant extract originally contained cannot be exerted due to the influence of other bases and the like.
In addition, there is a limit to the action and effect when a plant extract is mixed at a high temperature for the purpose of obtaining a higher effect, and conversely, due to the generation of a unique color and odor of the plant extract itself, the amount and dosage form However, there was a problem that product restrictions would occur.

[0007]

[Means for Solving the Problems] In view of such a situation,
The present inventors, as a result of intensive research in order to sufficiently bring out the skin action effect of the plant extract, by using a plant extract and superoxide dismutase in combination, for rough skin improving action and skin aging preventing action. The present invention has been completed by finding that the effects are synergistically exerted and that the action and effect originally possessed by the plant extract as a skin external preparation can be stably obtained.

That is, the present invention relates to (A) mica
It is intended to provide an external preparation for skin, which comprises one or more kinds of plant extracts selected from mocca and icrinin, and (B) superoxide dismutase.

The method for preparing the plant extract of the component (A) of the present invention is not particularly limited, but it is extracted, for example, using various suitable solvents at room temperature to under heating. Examples of the extraction solvent include water; lower monohydric alcohols such as methyl alcohol and ethyl alcohol; glycerin, propylene glycol,
Liquid polyhydric alcohols such as 1,3-butylene glycol;
One or more lower alkyl esters such as ethyl acetate; hydrocarbons such as benzene and hexane; ethers such as diethyl ether can be used. In particular, water, ethyl alcohol, glycerin, and a mixed solvent of one or more kinds of 1,3-butylene glycol are preferable. As the extraction conditions, a solvent is used in a volume ratio of 1 to 1000 times, especially 5 to 100 times, and a temperature of 4 ° C or higher, particularly 15 to 30 ° C, for 1 hour or longer, particularly for 1 to 3 days. It is preferable to carry out.

The plant extract obtained under the above conditions may be used as it is as an extracted solution, but if necessary, it may be subjected to a treatment such as concentration and filtration. These may be used alone or in combination of two or more.

The compounding amount of the plant extract in the external preparation for skin of the present invention is preferably 0.0001 to dry solid content.
It is 10% by weight (hereinafter, simply referred to as "%"), preferably 0.01 to 5%.

If the content is less than 0.0001%, the effect is not sufficiently exhibited, and if it exceeds 10.0%, no further increase in the effect is observed.

On the other hand, the other essential component of the present invention (B)
Superoxide dismutase (hereinafter referred to as "S
"OD") is a superoxide scavenging enzyme widely distributed in living bodies such as animals, plants and microorganisms, and its origin and acquisition method are not particularly limited.

As a method for producing this SOD, humans,
Even if extracted from animal tissues such as cows and dogs, cabbage,
It may be one extracted from plants such as cucumber and lettuce, one extracted from a microorganism, or one produced by genetic engineering. Further, if SOD is the main component, it need not be purified.

The content of these SODs in the external preparation for skin of the present invention is generally 0.00001% to 5%, preferably 0.001 to 3%. SOD content is 0.000
If it is less than 01%, sufficient effect may not be obtained, and even if it exceeds 5%, further increase in effect may not be observed, and adverse effects may occur on the formulation side. .

Further, the external preparation for skin of the present invention contains, in addition to the above-mentioned essential components, an aqueous component, a powder, a surfactant, which is used in ordinary external preparations for skin, within a range that does not impair the effects of the present invention.
Oil agents, moisturizers, alcohols, pH adjusters, preservatives, pigments, antioxidants, ultraviolet absorbers, thickeners, fragrances, cosmetic ingredients and the like can be appropriately added as necessary.

The external preparation for skin of the present invention comprises the essential component (A)
And component (B) may be blended to produce the composition according to a conventional method. And emulsion, cream, lotion, beauty essence, cleansing, pack, cleaning agent, foundation, etc.,
In addition, it can be applied as a skin external medicine for dispersion, granule, ointment or the like, for external use for quasi-drugs or for cosmetics.

[0018]

EXAMPLES Next, the present invention will be described in more detail with reference to test examples and examples, but the present invention is not limited thereto.

Test Example 1 Superoxide removing activity measurement test: To 20 parts by weight of dried mica, 80 parts by weight of 50 V / V% ethyl alcohol was added, and the mixture was extracted for 3 days with occasional stirring at room temperature and filtered to obtain a mica extract. Obtained. Using this as a sample, the superoxide removing activity was measured by the following measuring method. In addition, the activity was also measured for commercially available SOD and a combination of this with a squid extract. The results are shown in Table 1.

(Measurement method) Substrate solution in 2.4 ml of 0.05 M sodium carbonate buffer (pH 10.2) [3.0 mM xanthine (dissolved in 0.05 M sodium carbonate buffer)]
0.1 ml, 3.0 mM EDTA 0.1 ml, 0.15% (W
/ V) bovine serum albumin, 0.75 mM nitroblu-tetrazolium 0.1 ml and 0.1 ml of each sample are mixed, and 2
After standing at 5 ° C for 10 minutes, 0.1 ml of an enzyme solution [xanthine oxidase solution (diluted with purified water about 0.04 units / ml)] is added to start the reaction. 20 at 25 ° C
After incubating for 6 minutes, 6mM CaCl ₂ 0.1m
Stop the reaction by adding l. Then, the absorbance (A) at 560 nm is measured.

The absorbance (B) of the sample to which purified water was added instead of the specimen was used as a control, and 6 mM was used as the blank of each sample.
Absorbance of a sample to which 0.1 ml of xanthine oxidase was added after stopping the reaction by adding 0.1 ml of CaCl ₂ (C)
Was measured and the superoxide removal rate was calculated from the following formula.

[0022]

[Equation 1]

A: Absorbance of sample by enzyme reaction B: Absorbance of control enzyme reaction C: Absorbance of sample without enzyme reaction

[0024]

[Table 1]

* 1: Heat-extracted with 30 V / V% ethyl alcohol, filtered, concentrated under reduced pressure, and then aged in a cool place for aging. About 3% dry solids. * 2: Sigma; obtained from bovine red blood cells, 3.57
0 units / mg

As is clear from the results shown in Table 1, the squid extract alone had an SOD-like action, but SOD
It was shown that when used in combination with, it exerts a synergistic effect and is extremely effective in removing active oxygen.

This means that the external preparation for skin of the present invention in which the squid extract and SOD are used in combination is effective against the production of lipid peroxide, inflammation, blackening and aging due to the production of active oxygen in the skin by ultraviolet rays. This shows that it has an extremely high preventive effect.

Example 1 Emulsion: An emulsion was prepared according to the composition shown in Table 2 and the following production method, and its skin beautifying effect and skin aging preventing effect were examined. The results are shown in Table 5.

[0029]

[Table 2]

(Production Method) A. Ingredients (6), (7), (9) and (13) are heat mixed and maintained at 70 ° C. B. Heat-mix components (1) to (5) and (10),
Keep at 0 ° C. C. B is added to A and mixed, component (12) is added and the mixture is uniformly emulsified, cooled to 30 ° C., and components (8) and (1
1) is added and mixed uniformly to obtain an emulsion.

(Test Method) 15 women aged 28 to 58 were used as a panel, and an appropriate amount of the test emulsion was applied to the face after washing the face twice daily in the morning and at night for 12 weeks. The beautiful skin and anti-aging effect of the application were evaluated according to the following criteria.

[0032]

[Table 3] Skin beautifying effect: [Evaluation] [Contents] Effectiveness Dullness of the skin became inconspicuous. Somewhat effective The dullness of the skin became less noticeable. Ineffective No change from before use.

[0033]

[Table 4] Anti-aging effect on skin: [Evaluation] [Contents] Effectiveness The scalp and gloss of the skin were improved. Slightly effective Skin tone and gloss were slightly improved. Ineffective No change from before use. (result)

[0034]

[Table 5]

Example 2 Cream: A cream was prepared by the composition shown in Table 6 and the following production method, and its skin beautifying effect and skin aging preventing effect were examined.
The results are shown in Table 7.

[0036]

[Table 6]

* 3: Reflux extraction with 50 V / V% ethyl alcohol, filtration, concentration under reduced pressure, and standing in a cool place. About 2% dry solids. * 4: 10 kg of bovine placenta, finely chopped, added with double volume of 5% V / V ethyl alcohol aqueous solution, extracted at 37 ° C for 6 hours, and then purified by gel filtration, dialysis and the like. (Absorption capacity of about 2 kg) Protein content of about 1.0 to 1.5% * 5: 1 kg of cabbage is cut into small pieces, and the same amount of purified water is added to homogenize. Ammonium sulfate fractionation after filtration and dialysis treatment. (Yield about 10g) Protein amount about 2.0-4.0%

(Production Method) A. Components (1)-(7), (11) and (12) are mixed and heated to maintain at 70 ° C. B. Mix components (8) and (13) and heat to 70
Keep at ℃. C. After adding B to A, mixing and cooling (9) (1
0) was added and mixed uniformly to obtain a cream. (Test method) Same as Example 1 (Evaluation criteria) Same as Example 1 (Result)

[0039]

[Table 7]

As is clear from the results of Table 7, the product 2 of the present invention
Creams # 3 and # 3 lose the skin's “shine” and “swelling”,
It was effective in preventing and improving skin aging phenomena such as "dullness".

Example 3 Lotion:

[Table 8] (Prescription) (%) (1) Glycerin 5.0 (2) 1,3-butylene glycol 6.5 (3) Polyoxyethylene sorbitan monolaurate (20EO) 1.2 (4) ) Ethyl alcohol 8.0 (5) Mocca extract 1.0 (6) SOD ^{* 6} 0.5 (7) Preservative proper amount (8) Perfume proper amount (9) Purified water Remainder * 6: Sigma; human Obtained from red blood cells, 5.230
units / mg

(Production Method) A. Components (3), (4), (7) and (8) are mixed and dissolved. B. Components (1), (2), (5), (6) and (9) are mixed and dissolved. C. A and B were mixed and made uniform to obtain a lotion.

Example 4 Pack:

[Table 9] (Prescription) (%) (1) Polyvinyl alcohol 20.0 (2) Ethyl alcohol 20.0 (3) Glycerin 5.0 (4) Kaolin 6.0 (5) Mokka extract 0.05 ( 6) Placenta extract containing SOD ^{* 7} 0.01 (7) Preservative 0.2 (8) Perfume 0.1 (9) Purified water Remainder * 7: Freeze-dried product of Example 2

(Production Method) A. Mixing components (1), (3)-(5) and (9),
Heat to 70 ° C. and stir. B. Mix components (2), (7) and (8). C. B was added to A, mixed, and then cooled to uniformly disperse (6) to obtain a pack.

Example 5 Cleaning Charge:

(Table 10) (Prescription) (%) (1) Stearic acid 10.0 (2) Palmitic acid 8.0 (3) Myristic acid 12.0 (4) Lauric acid 4.0 (5) Oleyl alcohol 1.5 (6) Purified lanolin 1.0 (7) Perfume 0.1 (8) Preservative 0.2 (9) Glycerin 18.0 (10) Potassium hydroxide 6.0 (11) Iclinin extract ^{* 8} 0.5 (12) SOD ^{* 9} 0.05 (13) Purified water Residual amount * 8: 50 V / V% ethyl alcohol was added, and the mixture was extracted at room temperature for 3 days with occasional shaking, filtered, and concentrated under reduced pressure. About 6% dry solids. * 9: manufactured by Sigma; obtained from Horseradish,
2.150 units / mg

(Production Method) A. Mixing components (9), (10) and (13), 70
Heat to ℃. B. The components (1) to (6) and (8) are mixed and heated to 70 ° C. C. The above B was added to the above A, kept at 70 ° C. for a while, cooled to 50 ° C. after completion of the saponification reaction, and the component (7),
(11) and (12) were added and cooled to obtain a cleaning agent.

Example 6 Gel ointment:

Table 11 (Prescription) (%) (1) Carboxyvinyl polymer 1.0 (2) Triethanolamine 1.0 (3) 1,3-Butylene glycol 10.0 (4) Iclinin extract 0.01 ( 5) SOD 0.02 (6) Purified water Remaining amount

(Production Method) A. Components (1) and (3) to (6) are mixed and dissolved. B. Ingredient (2) was added to A, mixed and homogenized to obtain a gel ointment.

[0049]

EFFECT OF THE INVENTION Since the external preparation for skin of the present invention has an excellent action of removing active oxygen, it has a stable and excellent effect for improving rough skin and preventing skin aging. Therefore, the external preparation for skin of the present invention, for the production of lipid peroxide due to the generation of active oxygen in the skin by ultraviolet rays, inflammation, blackening, aging, etc.,
It has an extremely high preventive effect and is extremely useful in beauty and medicine.

Claims (1)

[Claims] 1. An external preparation for skin comprising (A) one or more kinds of plant extracts selected from mica, mocca and iclinin, and (B) superoxide dismutase.