JPH10229871A - Wiping tool for testing environmental microorganism - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a wiping tool for testing environmental microorganisms, capable of easily removing an excessive dilution liquid. SOLUTION: This wiping tool comprises a container main body 1 and a dropping pipette-having cap 2 for sealing the container main body 2. An impregnation member 4 for wiping up environmental microorganisms is disposed on the tip of a tube 3a of the dropping pipette 3. The tube 3a is inserted into the container main body 1.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a device for wiping environmental microorganisms used in medical institutions, food and pharmaceutical plants, and the like.

[0002]

2. Description of the Related Art A simple test method has been developed for environmental microorganism testing, and there are a conventional method mainly for culturing and a method using a reagent capable of determining the degree of contamination by microorganisms.

[0003] In the conventional method mainly for culturing, an operation of wiping adherent bacteria on the surface of an object with a cotton swab or the like is generally performed by a wiping method (also called a swab method). For example, an operation of wiping a surface of an object such as a device, an instrument, or a work table with a cotton swab moistened with a certain amount of a diluting liquid such as a phosphate buffer solution or a physiological saline solution is performed. This is placed in a synthetic resin container containing the same solution and shaken violently, or shaken with an ultrasonic wave having a weak wavelength that does not have sterilizing power. After diluting the exudate as needed and culturing, the colonies of the emerged bacteria are measured, and the number of bacteria in the wiped area is calculated.

[0004] The wiping method has an advantage that it can wipe not only a large area but also a finger, a bent portion, or a gap.

[0005]

However, a wiping device for collecting a specimen using a cotton swab or the like is a phosphate buffer solution or a physiological saline solution which is excessively impregnated in a wiping impregnant such as a cotton ball at the time of use. It is necessary to squeeze the diluent such as water. That is, in order to collect the adherent bacteria at the location to be inspected, if there is an excess of the diluent in the wiping impregnated body, the adherent bacteria cannot be collected, and the correct collection method cannot be obtained.

An object of the present invention is to provide a wiping device for environmental microorganism testing which can easily remove excess diluent.

Another object of the present invention is to provide a wiping device for environmental microorganism testing which can easily and inexpensively form a dropper from which excess diluent can be easily removed.

It is another object of the present invention to provide a wiping tool for environmental microorganism inspection which can form a dropper capable of easily removing an excessive dilution liquid by selecting an appropriate material.

[0009]

The invention according to claim 1 comprises a container body made of synthetic resin, and a lid with a dropper for sealing the container body, wherein the dropper of the dropper with the dropper is provided. The impregnating body for wiping is provided at the tip of the tube inserted into the container body.

[0010] As described above, when the lid is a lid with a dropper, and the tip of the pipe part inserted into the container body of the dropper with the dropper is provided with an impregnating body for wiping, Even if it is immersed in the diluting liquid in the container body during use and the diluting liquid is excessively impregnated in the wiping impregnating body, the air reservoir of the dropper is compressed and the internal air is blown out through the pipe of the dropper. This makes it possible to discharge excess diluent in the wiping impregnation body, and to easily hold an appropriate diluting liquid in the wiping impregnation body. Therefore, it is possible to correctly collect the adherent bacteria from the object surface by wiping.

The invention according to claim 2 is characterized in that the lid with a dropper is integrally formed of synthetic resin.

In this case, the lid with a dropper can be easily formed at low cost by integral molding. The container body and the lid with a dropper are preferably formed of a soft synthetic resin such as high-density polyethylene. Such a synthetic resin can withstand high-pressure steam sterilization and radiation sterilization.

The invention according to claim 3 is characterized in that the lid with a dropper is constituted by using the lid and the dropper as separate parts.

In this way, the lid and the dropper are
An appropriate material can be selected and formed depending on the application.

The invention according to a fourth aspect is characterized in that the impregnated body for wiping is made of a foamed synthetic resin.

The invention according to claim 5 is characterized in that the impregnating body for wiping is made of natural sponge.

The invention according to claim 6 is characterized in that the impregnated body for wiping is made of natural or chemical fiber.

[0018]

1 to 3 show a first embodiment of a wiping device for an environmental microorganism test according to the present invention.
This is an example.

This wiping device for environmental microorganism testing comprises a container body 1 and a lid 2 with a dropper for sealing the container body 1 and is inserted into the container body 1 of the dropper 3 of the lid 2 with a dropper. An impregnating body 4 for wiping is provided at the tip of the pipe 3a. Both the container body 1 and the lid 2 with a dropper are formed of a soft synthetic resin such as high-density polyethylene. These container body 1 and
The lid 2 with a dropper for sealing the container body 1 may be transparent or opaque, but as described later, the impregnating body for wiping inside the container body 1 with the lid 2 with a dropper attached will be described later. It is preferable that at least the container body 1 is transparent from the viewpoint of confirming the operation of pushing out the excessive dilution liquid in Step 4 and the step of collecting adherent bacteria.

The container body 1 is formed in a tubular shape with a bottom, and a female screw portion 1b is formed on the inner surface of the inlet 1a. A diluting liquid 5 such as a phosphate buffer solution or a physiological saline solution is accommodated on the bottom side in the container body 1.

The lid 2 with a dropper comprises a lid 6 and a dropper 3. The lid 6 is provided at the entrance 1 of the container body 1.
a, and a lid body 6b integral with the fitting portion 6a. The outer periphery of the fitting portion 6a is screwed to the female screw portion 1b of the inlet 1a of the container body 1. Male screw 6c
Are formed, and a plurality of non-slip grooves 6d are formed on the surface of the fitting portion 6a. The dropper 3 has a hollow air reservoir 3b and the above-described tube 3 integrated with the air reservoir 3b.
b and a vent-type impregnated body mounting portion 3c integrally formed at the tip of the tube portion 3b. As shown in FIG. 3, the air reservoir 3b of the dropper 3 in this example is formed by forming the fitting portion 6a of the lid 6 and the lid main body 6b integral with the lid 6a as a hollow flexible container. 6 is also used. The ventilation type impregnated body attachment part 3c is formed in a hollow elliptical container shape, and a large number of holes 7 are formed in the ventilation type impregnated body attachment part 3c. This hole 7
Are formed by many small round holes.

The impregnating body 4 for wiping is made of a sponge-like foamed synthetic resin such as foamed polyurethane or foamed polyethylene which is not deteriorated by the diluent 5 and has abrasion resistance. Such an impregnating body 4 for wiping is fitted into the ventilation type impregnating body mounting portion 3c of the dropper 3 and fixed with a water-insoluble synthetic adhesive.

In the wiping device for environmental microbial examination having such a structure, the tip side of the lid 2 with a dropper is inserted into the container body 1 in which the diluent 5 such as a phosphate buffer solution or a physiological saline solution is stored. As a result, the wiping impregnated body 4 is immersed in the diluting liquid 5, the fitting portion 6a of the lid 6 is fitted into the inlet 1a of the container body 1, and the inlet 1a of the container body 1 is
Is closed. By screwing the fitting part 6a of the lid part 6 into the inlet 1a of the container body 1, the male screw part 6c of the fitting part 6a is screwed with the female screw part 1b on the inner surface of the inlet 1a of the container body 1,
It will not come off. In this state, sterilization is performed with high-pressure steam or radiation. Storage and transport are performed in such a state.

At the sample collection site, the lid 2 with a dropper is removed from the container body 1, and the air reservoir 3 b of the dropper 3 is removed while the wiping impregnating body 4 has come out of the diluent 5 in the container body 1. By compressing and blowing out the internal air from the holes 7 of the ventilation type impregnated body mounting part 3c through the pipe part 3b of the dropper 3, the excess dilution liquid 5 in the wiping impregnated body 4 can be discharged. Thus, an appropriate dilution liquid 5 can be easily held in the impregnated body 4 for wiping. The compression of the air reservoir 3b may be repeated several times when one time is insufficient. When the amount of the diluent 5 impregnated into the wiping impregnated body 4 is set to an appropriate amount in this manner, it is possible to correctly collect adherent bacteria from the surface of the object by wiping.

Further, when the lid 2 with a dropper is integrally formed of a synthetic resin as in this embodiment, the lid 2 with a dropper can be easily formed at low cost by integral molding.
When the container main body 1 and the lid 2 with a dropper are formed of a soft synthetic resin such as high-density polyethylene as described above, it can withstand high-pressure steam sterilization and radiation sterilization.

Further, when the wiping impregnated body 4 is made of a foamed synthetic resin, the wiping impregnated body 4 which is not affected by heat or radiation caused by fraying or sterilization can be obtained.
If the wiping impregnated body 4 is not frayed, even if the surface of an object having a rough surface such as a plate or a zircon is wiped, fibers remain on the site after the wiping or the number of bacterial colonies due to the presence of the frayed fibers in the culture area is reduced. It can be prevented from hindering.

FIGS. 4 and 5 show modifications of the lid 2 with a dropper shown in FIG. In the lid 2 with a dropper, the holes 7 provided in the ventilation type impregnated body mounting portion 3c are formed by a plurality of slits. Other configurations are the same as those in FIG.

FIGS. 6 to 8 show a second embodiment of the wiping device for environmental microorganism testing according to the present invention.

This wiping device for environmental microorganism testing comprises a container main body 1 and a lid 2 with a dropper for sealing the container main body 1, and is inserted into the container main body 1 of the dropper 3 of the lid 2 with the dropper. The point that the impregnating body 4 for wiping is provided at the tip of the pipe portion 3a is the same as in the first example described above.

The container body 1 has a small-diameter portion 1c at the upper end having an inlet 1a, and a male screw portion 1d protruding from the outer periphery thereof. A diluting liquid 5 such as a phosphate buffer solution or a physiological saline solution is accommodated on the bottom side in such a container body 1.

In the lid 2 with a dropper, the lid 6 and the dropper 3 are integrally formed of a soft synthetic resin such as high-density polyethylene. The dropper 3 includes a hollow air reservoir 3b, the above-described tube 3a integrated with the air reservoir 3b, and a ventilation type impregnated body attaching portion 3c integrally formed at the tip of the tube 3a. ing. The ventilation type impregnated body attachment part 3c is formed in the shape of a hollow elliptical container as in the first example.
Has many holes 7 formed by small round holes. The lid 6 is formed integrally with the outer periphery of the tube 3a in a cap shape below the air reservoir 3b of the dropper 3. On the inner periphery of the cap-shaped lid portion 6, there is provided a female screw portion 6 e to be screwed with a male screw portion 1 d provided on the outer periphery of the small diameter portion 1 c at the upper end of the container body 1. A plurality of non-slip grooves 6 d are formed on the outer periphery of the lid 6.

The impregnating body 4 for wiping is made of a sponge-like foamed synthetic resin such as foamed polyurethane or foamed polyethylene which is not deteriorated by the diluting liquid 5 and has abrasion resistance similarly to the first embodiment. This impregnated body for wiping 4
Also, it is fitted into the attachment part 3c of the ventilated impregnated body of the dropper 3 and fixed with a water-insoluble synthetic adhesive. The wiping device for environmental microorganism test of the second example is also the first example,
Similar effects can be obtained in a similar manner of use.

FIGS. 9 and 10 show modifications of the lid 2 with a dropper shown in FIG.

In the lid 2 with a dropper shown in FIG. 9, the air reservoir 3b of the dropper 3 is formed separately from the pipe 3a. The air reservoir 3b is formed of, for example, rubber having good flexibility and resilience, and the tube 3a is formed of a soft synthetic resin such as high-density polyethylene as described above.
The air reservoir 3b is fitted to the end of the tube 3a,
Is stopped by a flange 3d provided at an end portion of the cover. When the air reservoir 3b and the tube 3a side of the dropper 3 are formed separately as described above, each side can be formed of an optimum material.

In the lid 2 with a dropper shown in FIG. 10, the lid 6 and the dropper 3 are formed separately, and the lid 6 is attached to the outer periphery of the pipe 3a of the dropper 3 by the screw connection portion 8. ing. Also in this case, the lid part 6 and the dropper 3 can be formed of an optimum material on each side. Also,
The color of the lid 6 can be changed as appropriate.

FIG. 11 shows a modification of the dropper 3. In this dropper 3, the ventilation type impregnated body attachment part 3c is formed in a tubular shape with the same outer diameter as the pipe part 3a,
In this ventilation type impregnated body attachment part 3c, the tip is a pipe part 3
The ventilation-type impregnated body mounting portion 3c is opened with the same inner diameter as that of FIG.

In each of the above examples, the case where the impregnating body 4 for wiping is made of a sponge-like foamed synthetic resin such as polyurethane foam or polyethylene is shown. However, the impregnating body for wiping 4 is made of natural sponge, natural or sponge. It can also be composed of chemical fibers. Such a natural impregnating body 4 for wiping can also be fixed to the ventilation type impregnating body mounting portion 3c of the dropper 3 with a water-insoluble synthetic adhesive.

[0038]

According to the wiping device for testing environmental microorganisms according to the present invention, the lid for sealing the container body is a lid with a dropper, and the pipe inserted into the container body of the dropper of the lid with the dropper. The tip of the part is provided with a wiping impregnating body, so even if it is immersed in the diluting liquid inside the container body during use and the wiping impregnating body is excessively impregnated with the diluting liquid, the air reservoir of the dropper By compressing and blowing out the air inside through the pipe of the dropper, excess diluent in the wiping impregnating body can be discharged, and an appropriate amount of diluting liquid can be easily retained in the wiping impregnating body. Can be. Therefore, it is possible to correctly collect the adherent bacteria from the object surface by wiping.

In the step of collecting microorganisms attached to the wiping impregnant, the dropper of the lid is inserted into the container body and the air reservoir of the dropper is pressed, so that compressed air is applied to the entire wiping impregnator. Microorganisms generated and adhered to the impregnating body for wiping can be efficiently collected, and the degree of contamination by environmental microorganisms can be grasped strictly.

[Brief description of the drawings]

FIG. 1 is a perspective view showing a first example of an embodiment of a wiping device for an environmental microorganism test according to the present invention.

FIG. 2 is a perspective view of a disassembled state of a container body and a lid with a dropper in the wiping device for environmental microorganism testing of the first example.

FIG. 3 is a longitudinal sectional view of a lid with a dropper shown in FIG. 2;

FIG. 4 is a perspective view showing a modification of the lid with a dropper shown in FIG. 2;

FIG. 5 is a longitudinal sectional view of FIG.

FIG. 6 is a perspective view showing a second example of the embodiment of the wiping device for environmental microorganism test according to the present invention.

FIG. 7 is a longitudinal sectional view of the wiping device for environmental microorganism test shown in FIG. 6;

8 is a longitudinal sectional view of a lid with a dropper of the wiping device for environmental microorganism test shown in FIG. 6;

9 is a longitudinal sectional view showing a modification of the lid with a dropper shown in FIG. 7;

FIG. 10 is a longitudinal sectional view showing a modification of the lid with a dropper shown in FIG. 7;

FIG. 11 is a vertical sectional view of a main part showing a modification of the dropper.

[Explanation of symbols]

 DESCRIPTION OF SYMBOLS 1 Container main body 1a Inlet 1b Female screw part 1c Small diameter part 1d Male screw part 2 Lid body with a dropper 3 Dropper 3a Pipe part 3b Air reservoir part 3c Ventilation type impregnating body attaching part 3d Flange 4 Wiping impregnating body 5 Diluent 6 Lid 6a Fitting part 6b Lid body part 6c Male screw part 6d Non-slip groove 6e Female screw part 7 Hole 8 Screw connection part

Claims (6)

[Claims] 1. A container body, and a lid with a dropper for sealing the container body, wherein a tip of a pipe portion of the dropper with the dropper inserted into the container body is a wiping impregnation body. A wiping device for environmental microbiological examination, characterized by comprising: 2. The wiping device for environmental microorganism testing according to claim 1, wherein the lid with a dropper is integrally formed of a synthetic resin. 3. The wiping device for environmental microorganism inspection according to claim 1, wherein the lid with a dropper is constituted by using a lid and a dropper as separate parts. 4. The wiping impregnation body is made of a foamed synthetic resin.
The wiping device for environmental microorganism testing according to item 1. 5. The wiping instrument for environmental microorganism testing according to claim 1, wherein the wiping impregnated body is made of natural sponge. 6. The wiping instrument for environmental microorganism testing according to claim 1, wherein the impregnated body for wiping is made of natural or chemical fiber.