JPH10219244A - Antioxidant medicine - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain an antioxidant agent improved in safety and stable resistance to oxidation, useful for products in the field of foods, cosmetics, phermaceutical preparations, agrochemicals, etc., by containing a component obtained through centrifugation, etc., of piscine testes. SOLUTION: This agent is prepared by containing a composition obtained through centrifugation and/or alcohol extraction of piscine testes originated from at least one kind selected from a group of herring order such as a herring, cod order such as a cod roe and an Alaska cod and Perucida such as a skipjack and a bonito, and preferably oils and fats-containing composition. The oils and fats-containing composition preferably contains tocopherol and/or L-ascorbyl fatty acid ester.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

The present invention relates to an antioxidant preparation derived from fish testes.

[0002]

2. Description of the Related Art Fats and oils, particularly fats and oils containing unsaturated fatty acids, are easily oxidized, and are the major causes of deterioration in quality and nutritional and functional properties. Among unsaturated fatty acids, linoleic acid, linolenic acid and arachidonic acid play an important role as essential fatty acids as nutrients. In recent years, EPA,
Attention has been paid to the physiological activities of polyunsaturated fatty acids such as DHA, and many foods and drinks containing these are on the market. However, since these unsaturated fatty acids have extremely low oxidative stability, it is necessary to add an antioxidant. At present, there is no antioxidant that can be stored for a long time at room temperature.

[0003] On the other hand, fish testes are generated in large quantities during the edible processing of fish eggs or fish meat parts such as kazunoko, tarako and salmon roe. It is said that the amount generated in 1995 was about 10,000 t for herring and cod and about 5000 t for salmon. A part (about 40 tons / year) of the basic protein (protamine histone) obtained by chemical decomposition at room temperature and then neutralized with an acidic aqueous solution is used as a food additive as an antibacterial agent for bacteria, lactic acid bacteria and membrane yeast. It is used. Further, a product obtained by separating and purifying a nucleic acid and a salt thereof are used for about 30 tons per year for pharmaceuticals or cosmetics. Most of these raw materials prepared by separation, processing and purification are salmonid testes. This is because salmon testes have a lipid content of 1% or less, and the final product is less likely to have an adverse effect on oxidative odor, color, and taste. In addition, testes derived from herring, cod, etc. are generated more than salmon due to large catches, but have a constituent lipid content of 4 to 6 and 2 to 4%, respectively, the color generated when preparing basic protein and nucleic acid products, He is shunned in terms of smell and deterioration of taste. For this reason, it is being considered to convert it to fertilizers or fertilizers. However, in fact, most of them are discarded materials, and these are unused resources for which effective utilization is an issue.

[0004]

SUMMARY OF THE INVENTION An object of the present invention is to provide a novel antioxidant preparation having a remarkable effect and high safety.

[0005]

Means for Solving the Problems According to the present invention, as a result of intensive studies to achieve the above object, the components obtained by centrifuging and / or alcohol extracting fish testes from the order Coleoptera, Codfish, and Perciformes are described. Hereafter, the testis component may have a strong antioxidant activity, and by forming these fish testis components into a composition in the form of an oil or an emulsion, the antioxidant activity can be maintained for a longer period of time. I learned that it would be possible. Further, by adding tocopherol, L-ascorbic acid, or a salt thereof or a fatty acid ester, protein, peptide, or the like to such an oil or emulsion, the antioxidant power can be further increased, and the above object is achieved. Was found.
The present invention has been completed based on such findings.

That is, the first aspect of the present invention is an antioxidant preparation characterized by containing a fish testis component,
The second is an antioxidant preparation characterized by containing an oil / fat-containing composition obtained by mixing the first component with an oil / fat.
The third is an antioxidant characterized by being an emulsified composition obtained by emulsifying the oil / fat-containing composition or a component thereof as an oil phase and an aqueous phase. The oil-and-fat-containing composition suitably contains tocopherol and / or L-ascorbic acid fatty acid ester, and the aqueous phase suitably contains at least one selected from the group consisting of L-ascorbic acid, salts thereof, proteins and peptides. Can be included. Hereinafter, the present invention will be described in detail.

[0007]

BEST MODE FOR CARRYING OUT THE INVENTION First, in the present invention, testes from the order Cerophaga, Cod, and Perciformes are used as fish testes. These testes are generated as by-products when processed for the purpose of obtaining fish eggs or fish meat, and can be easily obtained. As a specific fish species, herring is herring ( Clupea
harengus pallasi), Tara first is cod roe and eat the fish cod (Gadus morhua macrocephalus), mainly other Higedara (Lotella phycis of Alaska pollack which is the raw material of surimi (Theragra chalcogramma)), Codonopsis (Coelorhync
hus japonicus ), which is processed as a raw material for pasting. Perch eyes are skipjack ( Katsuwonus pelamis, skip
jac ) and bonito ( Sardo orientalis, bonito ) are processed for edible or insulin production. Other scorpionfish ( Hexagraos otakii ) and hockey ( Pleurogrammu )
s azonus ) is also processed, but it is difficult to obtain because of low catch. On the other hand, sockeye salmon ( Oncorhynchus nerk
a ), salmonids such as chum salmon ( Oncorhynchus keta ), and cabbage salmon ( Oncorhynchus gorbuscha ) are one-twentieth of the testis components derived from herring, cod, and perciformes.
Only the following activities were obtained. This is due to the fact that salmonids are buoyant eggs, whereas bonitos and the like are buoyant eggs floating on the surface of water, herrings and the like are sticky eggs on the surface of eggs, sticky films, sticky threads, etc.・ Sujiko and Kazunoko,
The shape and color of fish eggs such as codfish are different, and the lipid content in the testis is less than 1% for salmon milt, 2 to 4% for skipjack, cod lipid, and 4 to 6% for herring lipid. This is probably because the constituent materials and compositions are different.

[0008] Fish testes collected at a fishery processing plant are distributed in a raw or frozen state, and any of them can be used in the present invention. Fish testis components are produced as follows. After the fish testis has been thawed or heated, it is centrifuged at about 1000 rpm or more by a centrifuge to remove the precipitated portion. By this separation operation, a fish testis component having an antioxidant activity improved by a factor of 10 or more can be obtained. The testis component from which the precipitated portion has been removed is not used, and can be used as a dried product in a preparation using a heating dryer, a spray drying device or a freeze dryer. In the drying, a commonly used carrier such as cellulose, chitin and acid casein may be used in combination. Furthermore, it is also possible to use a dried alcohol extract for the preparation. The fish testis component is prepared by the above separation method.

The testis component-derived antioxidant substance includes substances known to have an antioxidant effect or an antioxidant synergistic effect, such as glutathione, polyamines, xanthines, proteins / amino acids, and phospholipids contained in the testis. Considering that, the above-mentioned known substances related to antioxidation in the herring testis component were quantified, and the antioxidant activity of the obtained preparation was measured.
The herring testis component showed only about 1/15 of the antioxidant activity. From this, it is considered that the active substance of the antioxidant preparation according to the present invention is an unknown novel substance or composition.

The first antioxidant preparation of the present invention is characterized by containing a fish testis component. The second antioxidant preparation of the present invention is a fat-and-oil-containing composition containing a fish testis component and a fat and oil. Here, the kind of fats and oils is not particularly limited, but fats and oils which are in a liquid state at ordinary temperature are simple to use. Specific examples of oils and fats applicable to the present invention include vegetable oils such as soybean oil, rapeseed oil, corn oil, safflower oil, cottonseed oil, sunflower oil, palm oil, linseed oil, cocoa butter, shea butter, olive oil, tallow, lard, etc. Animal oils and fats, fish oil can be used, and mono- or mixed-acid triglycerides of medium-chain fatty acids having 6 to 10 carbon atoms (for example, Nisshin Oil Co., Ltd., trade name: ODO), Acetin fat (triglyceride acetate), triglyceride oleate and the like can be mentioned. Of these, soybean oil, rapeseed oil, corn oil, and medium-chain fatty acid triglycerides are preferred. Such fats and oils can be used alone or in combination.

[0011] The amount of the antioxidant preparation according to the present invention to be added to the oil-and-fat-containing composition is 0.005 to 70% by weight, more preferably 0.02 to 70% by weight, based on the oil and fat, as a dry testicular component.
It is 50% by weight, most preferably 0.1 to 10% by weight. If it is less than 0.005%, the antioxidant effect is small, and
Even if it is added in excess of 0% by weight, a uniformly dissolved or dispersed oil-and-fat-containing composition cannot be obtained, and it is difficult to exhibit an antioxidant power commensurate with the amount added, which is uneconomical.

[0012] Tocopherol is contained in the oil-and-fat-containing composition in an amount of 0.001 to 10% by weight, preferably 0.1 to 5% by weight, and / or L-ascorbic acid fatty acid ester in an amount of 0.001 to 10% by weight, preferably Is 0.05-5
The addition by weight increases the antioxidant power of the oil-and-fat-containing composition further. Here, tocopherol is D-form, L-form or D-form.
The L-form, α, β, γ, and δ-tocopherol may be used alone or in any mixture, and a natural product or a synthetic product may be used. It is desirable that the content of δ-tocopherol is high, but practically, D-form α, β, γ and δ-tocopherol which are concentrated from by-products of deodorizing and purifying vegetable oils such as soybean oil, wheat germ oil, and rapeseed oil. Or a mixed tocopherol containing at least one of the following:
Even α-tocopherol in the body can be used. There is no problem even if such tocopherol is a mixture with fats and oils. As the L-ascorbic acid fatty acid ester, palmitic acid or stearic acid ester of L-ascorbic acid is commercially available, and these are preferable.

To prepare the oil-containing composition of the present invention,
The fish testis component, or this and tocopherol or / and L-ascorbic acid fatty acid ester as it is or after previously dissolving it in a solvent such as ethanol, isopropanol, butanol, or ether, is added to the fat or oil, and the mixture is added at room temperature or about Heat to 50 ° C, stir at 50 to 5000 rpm using a suitable stirrer or mixer such as a mixer or blender to uniformly dissolve or disperse, and remove solvent under reduced pressure if necessary. I just need. In order to adjust the properties of the final product, if necessary, a thickening agent such as oil-soluble 12-hydroxystearic acid, a wax such as candelilla wax, carnauba wax, paraffin wax, a coloring agent, a fragrance, etc. are blended. You may. Thus, an oil / fat-containing composition comprising a fish testis component and an oil / fat, and further, tocopherol and / or L
-The oil-and-fat-containing composition containing an ascorbic acid fatty acid ester is obtained (the second and third antioxidant preparations of the present invention).

Next, the third antioxidant preparation of the present invention is an emulsified composition obtained by emulsifying the oil / fat-containing composition or a component thereof as an oil phase and an aqueous phase. That is, (1) an oil-and-fat-containing composition containing a fish testis component and an oil or fat, or (2) the oil-and-fat-containing composition further containing tocopherol and / or L-ascorbic acid fatty acid ester, or (3). The fish testis component is dissolved in a solvent such as ethanol, isopropanol, butanol, or ether in advance and then added to the oil-and-fat-containing composition, and any of the oil-and-fat-containing compositions uniformly dissolved or dispersed and desolventized is used as an oil phase. An oil-in-water or water-in-oil emulsion composition obtained by emulsifying this and an aqueous phase, preferably in the presence of an emulsifier.

In the emulsified composition of the present invention, the emulsifier is a known one such as lauric acid, myristic acid, palmitic acid, stearic acid, oleic acid, linoleic acid, ricinoleic acid, 12-hydroxystearic acid, arachiic acid,
Propylene glycol monofatty acid ester, fatty acid mono- or diglyceride, sucrose mono-, di- or tri-fatty acid ester, sorbitan mono-, sesqui- or di-fatty acid having fatty acid residues such as behenic acid, tallow-decomposed fatty acid, coconut oil-decomposed fatty acid, and rapeseed oil-decomposed fatty acid Use of esters, ethylene oxide or / and propylene oxide adducts of the above fatty acids or fatty acid esters in an amount of 2 to 40 mol, and an ester emulsifier such as polyglycerin mono-, di- or tri-fatty acid ester having an average degree of polymerization of 2 to 15, especially 6 or 10. Can be.

A lecithin emulsifier such as soybean lecithin, rapeseed lecithin, egg yolk lecithin, phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol, phosphatidylinositol, or the like, which is fractionated, concentrated or chemically synthesized from the lecithin; Fatty acid soap emulsifiers such as potassium or sodium salts of fatty acids, ethylene oxide such as lauryl alcohol, tetradecyl alcohol, cetyl alcohol, stearyl alcohol, oleyl alcohol, aralkyl alcohol, behenyl alcohol, octylphenol, nonylphenol and / or propylene oxide An ether emulsifier such as a 30 mol adduct can also be used. In the present invention, one or more of these emulsifiers can be appropriately used, but fatty acid glyceride, sorbitan fatty acid ester, polyglycerin fatty acid ester and lecithin are particularly preferable.

The emulsifier is used in an amount of about 0.1 to 5% by weight based on the total weight of the emulsified composition, and the oil composition is used as an oil phase, and an oil phase / water phase = 5/95 to 95/5.
(Weight ratio, the same applies hereinafter), preferably in the case of an oil-in-water emulsion composition, preferably oil phase / water phase = 5/95 to about 60/40, and in the case of a water-in-oil emulsion composition, Is an oil phase / aqueous phase = about 40/60 to 95/5, and emulsified by a normal phase or phase inversion emulsification method. In the emulsified composition of the present invention,
Dispersed droplets (oil droplets in an oil-in-water emulsion composition, water droplets in a water-in-oil emulsion composition) have an average particle size of about 50 μm or less, preferably about 5 to 30 μm, more preferably about 5 to 30 μm.
It is better to set it to ２０20 μm. More than about 50μm and 60μ
When the average particle size is about m or more, it is difficult to maintain a stable emulsified state.

In preparing the emulsified composition,
When the aqueous phase contains at least one aqueous component selected from the group consisting of L-ascorbic acid, a salt thereof, a protein and a peptide, the antioxidant power of the emulsified composition can be further enhanced. In particular, the combined use of proteins is effective in enhancing the antioxidant power and stabilizing the emulsified state. In the whole emulsified composition, L-ascorbic acid and its salt may be used in an amount of 0.001 to 10% by weight, preferably 0.05 to 5% by weight, and protein may be used in an amount of 0.1 to 5% by weight.
-20% by weight, preferably 0.5-10% by weight,
The peptide is 0.1 to 10% by weight, preferably 0.5 to 1% by weight.
0% by weight is blended. If the addition amount of any of these aqueous components is small outside the above range, the antioxidant power of the fish testis component cannot be further enhanced, and conversely, even if the addition amount is increased beyond the above range, No further increase in antioxidant potential can be expected.

Among these aqueous components, L-ascorbic acid and its salts (sodium L-ascorbate, L-ascorbate,
-Hemicalcium ascorbate) may be a commercially available product. In addition, proteins and peptides may be derived from animals or plants. As for those of animal origin,
Proteins and peptides such as egg white, egg yolk, milk storage, meat extract, fishmeal, crabmeal, shrimp meal, feather meal, etc., are plant-derived, soybean, wheat, rapeseed,
Cottonseed, flaxseed, safflower, sunflower, sesame, peanut,
Examples include proteins and peptides extracted from palm, kapok, almond, walnut, and the like, but the present invention is not limited to these.

The peptide is obtained by hydrolyzing the animal or plant protein by a known method, that is, acid, alkali or protease, neutralization, heating, acid precipitation, aggregation, separation,
It can be subjected to treatments such as concentration and has a molecular weight of 50
0 to 50000, preferably 1000 to 1000
0. With a peptide having a molecular weight of less than 500, the desired effects of the present invention cannot be obtained, and the tendency of imparting taste to the emulsified composition increases, which may limit the use of the product. When the molecular weight exceeds 50,000, there is no difference in effect from the case where the protein is blended. Preferred examples of proteins and peptides include egg white, casein, concentrated soy protein, isolated soy protein, twain, gluten, and these peptides.

The emulsion composition of the present invention is prepared as follows. That is, the oil-and-fat-containing composition containing the testis component of fish, the oil and fat, and if necessary, the tocopherol and / or L-ascorbic acid fatty acid ester prepared by the above-mentioned method is used as the oil phase.
An aqueous solution in which at least one aqueous component selected from the group consisting of ascorbic acid, a salt thereof, a protein and a peptide is dissolved or dispersed is used as an aqueous phase, and preferably, part or all of the emulsifier is used in the oil phase or the aqueous phase. Add to phase. At this time, it is preferable that the lipophilic emulsifier is dissolved in the oil phase, the hydrophilic emulsifier is dissolved in the aqueous phase, and when the lipophilic and hydrophilic emulsifiers are used in combination, the respective phases are similarly dissolved.

Each of the oil phase and the aqueous phase is heated at room temperature or, if necessary, at about 70 ° C.
Stirring with a homogenizer, Hiscotron, Ajihomomixer, etc., using a mixer, for example, at a rotation speed of 1000 to 1000
While stirring at 0 rpm for 10 to 30 minutes, the oil phase is preferably added to the aqueous phase for an oil-in-water emulsion composition, and the aqueous phase is added to the oil phase for a water-in-oil emulsion composition. Each is emulsified.

The emulsified composition of the present invention is prepared by dissolving or dissolving the essential components of the oil / fat composition, that is, the fish testis component and the oil / fat, or the component and tocopherol and / or L-ascorbic acid fatty acid ester in advance. Alternatively, it can be prepared by directly mixing and emulsifying this with water, an aqueous component, an emulsifier, and the like. Further, as the other aqueous phase component, as necessary, carboxymethyl cellulose, guar gum, xanthan gum, sodium alginate, thickening or gelling agents such as carrageenan, coloring agents,
Flavors and the like may be blended. Such an emulsified composition can be solidified or powdered by means such as freeze-drying and spray-drying.

Thus, an oil-in-water or water-in-oil type emulsified composition obtained by emulsifying a fish testis component, an oil and fat, and water, or a component containing tocopherol and / or L-ascorbic acid fatty acid ester, and further comprising L -The emulsified composition containing at least one selected from the group consisting of ascorbic acid, its salts, proteins and peptides is obtained (third antioxidant preparation of the present invention). Thus, an antioxidant preparation containing the testis component of fish and an oil or fat is obtained.

As described above, the antioxidant preparation of the present invention comprises an oil-containing composition (oil) containing the testis component of fish together with oil and fat.
Or an oil-in-water or water-in-oil emulsion composition (emulsion) together with water, or tocopherol, L-ascorbic acid fatty acid ester, L-ascorbic acid, a salt thereof, protein, peptide, etc. It is a blended product that can maintain and enhance the antioxidant power of fish testis components, so that it can be used in fisheries, livestock, food and drink, health food, therapeutic food, cosmetics, pharmaceuticals, chemicals (rubber, plastic, etc.) It can be used in the field.

[0026]

EXAMPLES Next, the present invention will be described more specifically with reference examples and examples. [Reference Example 1] Cod, herring, skipjack and salmon testes were each centrifuged at 3000 rpm for 30 minutes from 1 kg each to remove precipitates, and then spray-dried to obtain testis extracts. The recovered amounts were 53 g, 48 g, 32 g and 42 g, respectively. Salmon testis components are further ultrafiltered (MC8 manufactured by Amicon)
Was used to obtain a fraction having a molecular weight of 10,000 or less, followed by freeze-drying to obtain 5.2 g of a low-molecular-weight fraction of salmon testes. On the other hand, when components related to antioxidation of the herring testis component were analyzed, glutathione 0.0012% by weight, polyamines 0.018% by weight,
Xanthine 0.0048% by weight, phospholipids 3.5% by weight
And 26% by weight of protamine histone. The breakdown of polyamines is putrescine 0.0084% by weight, spermidine 0.0060% by weight and spermine 0.00%.
It was 36% by weight. The breakdown of the phospholipids was 0.9% by weight of phosphatidylcholine, 0.4% by weight of phosphatidylethanolamine, and 0.8% by weight of phosphatidylinositol. A reagent mixture was prepared by mixing a reagent (manufactured by Sigma) in an amount corresponding to these and a starch degraded product (manufactured by Matsutani Chemical Co., trade name: H-PDX) for the other parts. In addition, a herring testis obtained by freeze-drying the herring testis as it was was prepared as a control. Using sardine oil, 2000 ppm of the testis component and the control sample according to the present invention were added.
The antioxidant power was measured at 00 ° C. in a CDM test (using Rancimat E679, manufactured by Metrohm Shibata). Table 1
Shows the results.

[0027]

[Table 1]

It was confirmed that the cod, herring, and skipjack testes components according to the present invention had very strong antioxidant activity.
It was found that the antioxidant activity was low without the centrifugation operation, and that only a known testis-containing antioxidant and a mixture thereof gave only a slight antioxidant activity. In addition, salmon-derived testis components and salmon-derived testis low molecular weight fractions hardly exhibited antioxidant activity.

[Reference Example 2] 1 kg of a testis mixture derived from herring, cod and bonito was heated at 80 ° C. for 30 minutes, and then centrifuged to remove the precipitate, and 5% by weight of Avicel (manufactured by Asahi Kasei Corporation).
The mixture was freeze-dried to obtain 65 g of testis component. Further, 90% aqueous ethanol was added to 5 g of the obtained testis component.
After adding 00 ml and stirring, the mixture was filtered and the filtrate was dried under reduced pressure to obtain 22 g of testis extract. Using sardine oil, add testis centrifugation component or testis alcohol extract, 100 ° C
The antioxidant power was measured by a CDM test (using Rancimat E679, manufactured by Metrohm Shibata). Table 2 shows the results.

[0030]

[Table 2] The antioxidant activity of the testis centrifuged component, the testis extract, and the alcohol extract according to the present invention was observed when added from 200 ppm to 70%.

Reference Example 3 To 1 kg of a testis mixture derived from herring, cod and bonito was added 2 liters of 90% (v / v) ethanol, and the mixture was stirred at 4500 rpm for 5 minutes with a homogenizer and centrifuged to remove precipitates. Drying under reduced pressure gave 52 g of testis alcohol extract.

Reference Example 4 Safety Test A 5-week-old ddY male mouse was fed on a solid feed (CLEA Japan, CE
-2) Mice weighing 30 to 34 g that had been preliminarily reared for one week by freely ingesting water (tap water) were used. A group of 10 mice deprived on the night before administration was treated with herring, cod and bonito testis centrifuged components 50% aqueous solution described in Reference Example 2 and 50% testis ethanol extract aqueous solution described in Reference Example 2 and Reference Example 2. The 50% aqueous testis alcohol extract described in 3 was orally administered by a gastric tube. In addition, 10 control groups were kept and the follow-up and autopsy of all cases were performed over the next two weeks. As a result, no fatalities were observed and no abnormalities were noted in any of them. The weight gain in the sample group showed a decreasing trend in the first week,
There was no significant difference from the control group. Necropsy showed no major organ abnormalities. As a result of the acute test, no death was observed even at the dose of 20 g / kg. The above safety test results revealed that the fish testis component of the present invention had extremely high safety.

[0033]

【Example】

Example 1 D-α, β, γ, and δ-mixed tocopherol containing soybean oil and testicular component derived from herring prepared in Reference Example 1 in 100 g of soybean white oil (Reagent TYPEV, manufactured by Sigma)
A solution prepared by dissolving 2% by weight of ethanol in 10 ml of ethanol was added, and the mixture was mixed using a paddle mixer at 3000 rpm for 3 minutes to dissolve testis components. The oil and fat composition of the testis component and the testis component alone obtained by evaporating the ethanol under reduced pressure are each placed in a brown sample bottle, capped, and stored in a thermostat at 40 ° C. for 3 months, 6 months and 12 months. did. Thereafter, the antioxidant activity against Alaska pollack liver oil was compared using the oil / fat composition and the testis component alone stored for each predetermined period. In other words, each testis component was added so that the content thereof became 200 ppm with respect to Alaska pollack liver oil. In the case of a testis component alone, 1/5 weight of the tocopherol was further mixed and subjected to a forced deterioration test (AOM test). went. That is, air was applied to the sample at 97.8 ± 0.2 ° C. for 2.3 times.
Blowing was performed at a flow rate of ml / sec, sampling was performed at regular intervals, and the peroxide value (POV) was measured.
The time to reach 00 was determined. Table 3 shows the results.

[0034]

[Table 3] Note) The values in the table are the time until the peroxide value of the target fats and oils reaches 100.

From the data shown in Table 3, it was clarified that the testicular component oil-and-fat-containing composition of the present invention maintained high antioxidant activity even after being used for a long period of time. On the other hand, the antioxidant power of the testis component itself decreases with time. In addition, comparing with the data of Table 4 (Example 2) described later, it was also revealed that the testis component oil-and-fat-containing composition using tocopherol in combination has enhanced antioxidant power.

Example 2 In Example 1, the antioxidant against Alaska pollack liver oil was obtained using the testicular component-containing composition containing cod derived from cod and the testis component alone prepared in Reference Example 1 prepared and stored in Reference Example 1 without using tocopherol. Forces were compared in the manner described in Example 1. From the results (see Table 4), it was also confirmed that the testicular component oil / fat composition of the present invention can suppress the reduction of the antioxidant power even after long-term storage.

[0037]

[Table 4] Note) The values in the table are the time until the peroxide value of the control fats and oils reaches 100.

Example 3 Mixed medium-chain fatty acid triglyceride of caprylic acid and capric acid to which 10% by weight of herring, cod and skipjack testes mixed extract prepared in Reference Example 2 was added (manufactured by Nisshin Oil Co., Ltd. (Name: ODO) 100 g of DL-α-tocopherol (manufactured by Kanto Chemical Co., Ltd., reagent) 0.4% by weight and L
-0.05% by weight of ascorbic acid stearate (trade name: vitamin C stearate, manufactured by Ogawa Koran Co., Ltd.) was added, and the mixture was mixed at 3,000 rpm for 5 minutes using an azihomomixer to prepare a translucent solution. . The testis extract oil-and-fat-containing composition was kept warm at 50 ° C. and stirred with a homogenizer while mixing tetraglycerin-condensed ricinoleate (manufactured by Sakamoto Pharmaceutical Co., Ltd., trade name: SY Glister C)
R-310) was added to the oil-and-fat-containing composition in an amount of 2% by weight, and sodium L-ascorbate and egg white peptide (made by Kewpie Co., Ltd., trade name: egg white peptide EP-1, average molecular weight: 1100) were further added to water. 100 ml of an aqueous solution in which 0.4% by weight of each was dissolved was heated to 50 ° C. and added sequentially. After mixing at 7000 rpm, the mixture was further mixed with a homogenizer at 10000 rpm for 2 minutes to extract a W / O testis extract. An emulsified composition was produced as a trial.

The emulsified composition and testis component alone were heated at 40 ° C.
And stored for 3, 6 and 12 months. The testis component alone stored for a predetermined period was used as the testis component emulsion in the same manner as described above. Each emulsified sample was sworded and the testis ethanol extract content was 200 ppm with respect to the refined oil.
, And the same forced deterioration test as in Example 1 was performed. The antioxidant powers were compared with each other until the POV reached 100 (see Table 5).

[0040]

[Table 5] Note) The values in the table are the time until the peroxide value of the control fats and oils reaches 100.

From Table 5, it was confirmed that the testis extract emulsion composition of the present invention maintained high antioxidant activity even after long-term storage. The emulsified state was also stable. Table 6 described later
When compared with the data of (Example 4), L
It was also observed that the use of sodium ascorbate and egg white peptide further enhanced the antioxidant power.

Example 4 In Example 3, a mixture of herring, cod and bonito testis extract (excipient) prepared in Reference Example 2 prepared and stored in a similar manner without using sodium L-ascorbate and egg white peptide Example 3 shows the antioxidant power of the emulsified composition prepared in the same manner using the emulsified composition containing avicel) and the testis mixed component alone stored for a predetermined period of time against purified sardine oil.
The comparison was performed by the described method. From these results (see Table 6), it was also confirmed that the emulsion composition of the present invention can suppress the reduction of the antioxidant power even after long-term storage. The emulsified state was also stable.

[0043]

[Table 6] Note) The values in the table are the time until the peroxide value of the control fats and oils reaches 100.

Example 5 1% by weight of a mixed extract of herring, cod and skipjack testes prepared in Reference Example 2, D-γ-tocopherol (manufactured by Sigma,
Reagent) 0.2% by weight (all ratios to fats and oils) was added to 100 ml of soybean / rapeseed mixed salad oil (manufactured by Nisshin Oil Co., Ltd.), and the mixture was added with a paddle mixer at 4000 rpm for 3 minutes.
The mixture was sufficiently stirred and mixed for minutes to dissolve to give an oil and fat. On the other hand, isolated soy protein (manufactured by Nisshin Oil Co., Ltd., trade name: Solpy 50)
Soy lecithin (trade name: Lecithin DX, manufactured by Nisshin Oil Co., Ltd.) as an emulsifier was also added to an aqueous solution containing 0.3% by weight of water to 0.3% by weight of water to obtain an aqueous phase. 40
The oil phase was added dropwise at a flow rate of 20 ml / min while stirring the aqueous phase heated to 0 ° C with a homomixer (5000 rpm), followed by mixing at 8700 rpm for 3 minutes to obtain an O / W-type mixed testis extract emulsion composition. Was prototyped.

A preservation test of β-carotene was carried out using the emulsified composition stored at 60 ° C. for 6 months and the same O / W-type mixed testis extract emulsified composition immediately after trial production. That is, isomerized sugar 20% by weight, citric acid 1% by weight, ethanol 2% by weight, water 77% by weight, β-carotene 0.3mg%
Was prepared, and 0.01% by weight of each emulsified composition was added thereto to prepare a juice.
A storage test was performed at 0 ° C. for 2 months. Samples were taken over time, and the content of β-carotene was measured as the absorbance at a wavelength of 450 nm, and the residual ratio was calculated by comparing the value at the start of storage. As a result, the residual ratio of β-carotene was as shown in Table 7.

[0046]

[Table 7]

[0047]

According to the present invention, an antioxidant preparation containing a fish testis component as an active ingredient can be provided. The antioxidant is obtained by centrifuging and / or alcohol extracting fish gonads. The antioxidant preparations of the present invention have strong antioxidant activity and high safety, food, cosmetics, pharmaceuticals,
It is expected to be applicable to products in fields such as agrochemicals.

Claims (6)

[Claims] 1. An antioxidant preparation comprising a component obtained by centrifuging and / or extracting alcohol from a fish testis. 2. An antioxidant preparation comprising an oil-and-fat-containing composition containing a component obtained by centrifuging and / or alcohol-extracting a fish testis and an oil or fat. 3. The antioxidant preparation according to claim 1, wherein the testis of the fish is derived from one or more of the order Cerophies, Cods, and Perciformes. 4. The antioxidant preparation according to claim 2, wherein the oil-and-fat-containing composition contains tocopherol and / or L-ascorbic acid fatty acid ester. 5. An antioxidant preparation, which is an emulsified composition obtained by emulsifying the oil or fat composition according to claim 2 or 4 as an oil phase and an aqueous phase. 6. The antioxidant preparation according to claim 5, wherein the aqueous phase contains at least one selected from the group consisting of L-ascorbic acid, a salt thereof, a protein and a peptide.