JPH10218719A - Antimicrobial agent - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain a naturally occurring antimicrobial agent having no adverse effects such as irritation, excellent in medicinal effects and stability, by including an extract of a plant of the genus Rhopala of the family Roupala as an active ingredient. SOLUTION: A wood part, a core part, a bark part, a branch part or a leaf part of a plant of the genus Rhopala of the family Roupala [e.g. R. montana] is extracted with a solvent (e.g. methanol) at 3-70 deg.C, optionally purified, diluted, concentrated, dried, etc., to give an antimicrobial agent comprising the plant of the genus Rhopala of the family Roupala. The antimicrobial agent in an amount of 0.001-20wt.% is optionally formulated with another antimicrobial agent, glycerol, a polymer thickening agent, a perfume, an oil, an ultraviolet light absorber, a chelating agent, a dyestuff, etc., to give a quasi medicine, a cosmetic or a toiletry product such as a dentifrice, a shampoo, an oily ointment, a cream, a toilet lotion, a soap, a body cosmetic, etc.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

TECHNICAL FIELD The present invention relates to a natural antibacterial agent suitably used for pharmaceuticals, quasi-drugs, cosmetics, toiletries and the like.

[0002]

2. Description of the Related Art Antibacterial agents are used in various fields such as skin diseases caused by microorganisms, prevention of decay of cosmetics, and disinfection of fingers.

[0003] Most of the conventionally used antibacterial agents are synthetic products, which cause irritation to the skin and the like, and cause many side effects such as causing skin disorders.
Due to such safety problems, the amount used and the dosage form that can be blended are limited, and at present it is not possible to exert the effect sufficiently. Further, natural antibacterial agents have drawbacks such as weak action and instability of components, and no satisfactory antibacterial agents have been obtained.

[0004] The present invention has been made in view of the above circumstances,
An object of the present invention is to provide a natural antibacterial agent which has no irritation or other side effects and is excellent in efficacy and stability.

[0005]

Means for Solving the Problems and Embodiments of the Invention The present inventors have achieved the above-mentioned object and have conducted intensive studies on the antibacterial properties of natural products.
oupala, Rhopala) The solvent extract of the plant is
Staphylococcus epidermidis (Staphy
lococcus epidermidis, Staphylococcus aureus
us aureus, Propionibacterium acnes
s) and has excellent antibacterial properties, and the solvent extract of the above-mentioned plants of the genus Roxaceae is effective as an antibacterial agent for pharmaceuticals, quasi-drugs, cosmetics, toiletry products, etc., especially for skin cosmetics and hair cosmetics It was found that the present invention can be used, and the present invention was completed.

[0006] That is, the present invention provides an antibacterial agent containing a solvent extract of a plant belonging to the genus Roeparaceae of the family Elderaceae as an active ingredient.

Hereinafter, the present invention will be described in more detail.
The genus Roupala (Rhupala, Rh) used in the present invention
opala) plants include Roupara montana (R.m.
ontana), Rowpara brasilensis (R. br)
asiensis), Raupara glabrata (R.
glabrata), low para heterophylla (Rh)
terophylla), R. macrophylla, R. martiii, R. parabari (R. ov)
alis) and R. tomenta
osa) and the like, Staphylococcus epidermidis (Stapylococcus epi)
dermidis), Staphylococcus aureus, Propionibacterium acnes (Propionib)
Rowpara-montana is most preferred in that an extract having particularly excellent antibacterial properties against A. acnes can be obtained. Loupara Montana is a woody plant native to the Brazilian Amazon basin (height 15-20)
m), used for furniture, etc.
aeira) and Carvalho.

[0008] The above-mentioned plant has its xylem, heartwood, bark,
Branches and leaves can be used.

The plant extract may be an extract or an extract separated and purified from the extract. The extract can be obtained by solvent-extracting the dried or crushed plant. In this case, the extraction is performed using a solvent such as methanol, ethanol, butanol, such as alcohols, hexane, heptane, aliphatic hydrocarbons such as cyclohexane, aromatic hydrocarbons such as benzene, esters such as ethyl acetate, ketones such as acetone,
It can be carried out by using water alone or as a mixture.
Among them, particularly preferred are methanol and ethyl acetate. Further, the extraction treatment can be carried out by a usual method at a temperature of usually about 3 to 70 ° C. The obtained extract may be used as it is, or may be used as a diluent or as a concentrated extract, or may be prepared as a dry powder by freeze-drying or the like, or may be prepared in a paste form.

[0010] Separation and purification of the active ingredient from the extract can be achieved by purifying the extract with a solvent by column chromatography or liquid chromatography.

The form of the dosage form to which the antibacterial agent of the present invention can be added is arbitrary, and includes pharmaceuticals, quasi-drugs, cosmetics,
It can be widely used for toiletry products and the like. For example, dentifrice, shampoo, rinse, oily ointment, aqueous ointment, cream, serum, lotion, emulsion, lotion,
It can be used as a pack, soap, facial cleanser, makeup, body cosmetics, etc., and is particularly suitable for skin cosmetics and hair cosmetics.

Known components according to the above-mentioned dosage forms can be blended. Such components include, for example, glycerin, polymeric thickeners, fragrances, oils, UV absorbers, chelating agents, pigments, and the like.

Further, it is used in combination with other antibacterial agents and preservatives, for example, alcohols, organic acids, chlorhexidine gluconate, sodium benzoate, paraoxybenzoic acid, methyl paraoxybenzoate, ethyl paraoxybenzoate, propyl paraoxybenzoate and the like. Can be used.

The amount of the antibacterial agent of the present invention varies depending on the dosage form, but is generally preferably 0.001 to 20% by weight. Particularly, when it is blended as a medicinal ingredient of a drug, a quasi-drug, etc., it is particularly preferably 0.1 to 10% by weight, and it is blended for the purpose of preventing microbial contamination in a formulation such as a preservative of cosmetics and toiletry products. 0.0 if
1-2% by weight is particularly preferred. If the compounding amount is less than 0.001% by weight, the intended effect cannot be exerted, and the production may become difficult depending on the dosage form. Therefore, it is better not to exceed 20% by weight.

[0015]

The antibacterial agent of the present invention has no side effects such as skin irritation, exhibits a stable and excellent antibacterial effect, and has a very wide range of applications.

[0016]

EXAMPLES Hereinafter, the present invention will be described in detail with reference to Preparation Examples, Examples, Comparative Examples and Formulation Examples, but the present invention is not limited to the following Examples. In the following examples,% indicates% by weight.

[Preparation Example] (Preparation of wax para-montana extract) A 10-fold amount (w / v) of methanol was added to 1 kg of shredded material of wax para-montana heartwood, and extracted for 2 hours. Next, the mixture was filtered, and methanol was distilled off under reduced pressure to obtain 30 g of wax para-montana extract.

[Example 1] (Antibacterial activity test) A Mueller Hinton agar medium was used as a medium and sterilized in an autoclave at 120 ° C for 20 minutes. The obtained agar medium was kept at a temperature of about 50 ° C., inoculated aseptically with pre-cultured bacteria, stirred, and gently poured into a sterile petri dish to prepare an agar plate.

40 μg / m of wax para montana extract
l, ethanol solution 25μl with filter paper disc (diameter 8m)
m) and adhered on the agar plate as described above.
The cells were aerobically cultured at 24 ° C. for 24 hours, and the antibacterial activity was measured based on the size of the inhibition circle.

The bacterium is Staphylococcus epidermidis (Staphylococcus epidermidis).
is ATCC12228), Staphylococcus aureus (Staphylococcus aureu)
s209P), Propionibacterium acnes A
TCC11827) was used.

In addition, Propionibacterium acnes (Propionibacterium acnes)
The antibacterial activity test against ATCC 11827) was performed using GAM agar medium, after the same operation as above, anaerobic culture was performed at 37 ° C. for 48 hours, and the antibacterial activity was measured by the size of the inhibition circle. Table 1 shows the results.

[0022]

[Table 1]

Examples 2 to 9 and Comparative Example 1 The following experiments were carried out using samples (lotion) having the compositions shown in Table 2. A Mueller Hinton agar medium was used as a medium, and sterilized in an autoclave at 120 ° C. for 20 minutes. The obtained agar medium was kept at a temperature of about 50 ° C., inoculated aseptically with pre-cultured bacteria, stirred, and gently poured into a sterile petri dish to prepare an agar plate.

25 μl of the sample was placed on a filter paper disc (diameter 8 m).
m) and adhered on the agar plate as described above.
The cells were aerobically cultured at 24 ° C. for 24 hours, and the antibacterial activity was measured based on the size of the inhibition circle.

The bacterium is Staphylococcus epidermidis (Staphylococcus epidermidis).
is ATCC12228), Staphylococcus aureus (Staphylococcus aureu)
s209P), Propionibacterium acnes A
TCC11827) was used.

It should be noted that Propionibacterium acnes (Propionibacterium acnes)
The antibacterial activity test against ATCC 11827) was performed using GAM agar medium, after the same operation as above, anaerobic culture was performed at 37 ° C. for 48 hours, and the antibacterial activity was measured by the size of the inhibition circle. Table 2 shows the results.

[0027]

[Table 2] From the above results, the strong antibacterial activity of the extract of the genus Lupra was proved.

Next, a formulation example is shown. [Formulation Example 1] Toothpaste Precipitable silica 25.0% Sorbit 25.0 Glycerin 25.0 Polyvinylpyrrolidone 1.0 Lauryl polyglycerin ester 1.0 Polyoxyethylene (60 mol) Sorbitan monolaurate 0.5 Saccharin sodium 0 .2 Core extract of wax para-montana 0.2 Extract of branch of wax para brasilensis 0.2 Perfume 1.0 Purified water Fuel gauge 100.0%

[Formulation Example 2] Mouthwash Sorvit 10.0% Ethanol 5.0 Polyoxyethylene (60 mol) hydrogenated castor oil 0.1 Sucrose monopalmitate 0.2 Saccharin sodium 0.2 Rowpara Montana branch extract Thing 0.5 wax para glabrata heartwood extract 0.5 perfume 0.7 purified water fuel gauge 100.0%

[Formulation Example 3] lotion glyceryl ether 1.5% polyoxyethylene (20 mol) hydrogenated castor oil 1.5 sorbitan monostearate 1.0 squalane 7.5 dipropylene glycol 5.0 waxpara-montana branch Extract 1.0 wax para heterophylla branch and leaf extract 1.0 perfume 0.5 purified water remaining amount 100.0%

[Formulation Example 4] Ointment White petrolatum 40.0% Cetanol 18.0 Sorbitan sesquioleate 5.0 Lauromacrogol 0.5 Ethyl parahydroxybenzoate 0.02 Butyl paraoxybenzoate 0.02 Rowpara Montana branch and leaf Part extract 2.0 Low Para-Marti heartwood part extract 2.0 Perfume Micro- purified water Fuel gauge 100.0%

[Formulation Example 5] Cream Propylene glycol 6.0% Dibutyl phthalate 19.0 Stearic acid 5.0 Glycerin monostearate 5.0 Sorbitan monostearate 12.0 Polyethylene sorbitan monostearate 38.0 edetic acid Sodium 0.03 Roepara montana heartwood extract 2.0 Roepara ovalis bark extract 2.0 Fragrance Micro- purified water Fuel gauge 100.0%

[Formulation Example 6] Hair shampoo lauryl sulfate triethanolamine 15.0% lauryl sulfate monoethanolamide 5.0 magnesium stearate 1.5 liquid lanolin 1.0 wax para-montana heartwood extract 1.0 wax para Tomentosa branch and leaf extract 1.0 Fragrance, pigment 0.2 Water Fuel gauge 100.0%

[Formulation Example 7] Hair rinse Stearyl dimethylbenzylammonium chloride 2.0% polyoxyethylene cetyl ether 1.2 polyoxyethylene lanolin ether 2.0 propylene glycol 5.0 citric acid 0.1 sodium citrate 0.1 Butyl paraoxybenzoate 0.01 Methyl paraoxybenzoate 0.02 Wax para-montana branch / leaf extract 1.0 Fragrance 0.2 Water Fuel gauge 100.0%

[Formulation Example 8] Antiperspirant Aluminum chlorohydroxide 20.0% Propylene glycol 5.0 Ethanol 10.0 Raw wax / Montana heartwood extract 0.5 Raw wax / Macrophylla bark extract 1.0 Perfume 0.2 water fuel gauge 100.0%

[Compounding Example 9] Hair restorer 90% ethanol 89.5% wax para-montana heartwood extract 3.0 Liquid paraffin 5.0 Trimethylglycine 2.0 Flavor 0.5 Total 100.0%

[Formulation Example 10] Body shampoo Potassium laurate 10.0% Potassium myristate 10.0 Palm oil fatty acid diethanolamide 3.0 Lauryl dimethylamine oxide 1.0 Propylene glycol 6.0 Raw para-montana heartwood extract 0.5 Hydroxypropyl methylcellulose 0.5 Ethylene glycol distearate 1.0 Tetrasodium edetate tetrahydrate 0.1 Fragrance 1.0 Purified water Fuel gauge 100.0%

[Formulation Example 11] Bath agent Sodium sulfate 40.0% Sodium bicarbonate 49.0 Roepara montana bark extract 5.0 Roepara brasilensis heartwood extract 5.0 Perfume 1.0 Total 100.0 %

Claims (1)

[Claims] 1. An antibacterial agent comprising, as an active ingredient, an extract of a plant belonging to the genus Roeparaceae of the family Papilionaceae.