JPH10179139A - New-strain of drechslera monoceras and controlling agent for weed containing the same - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain Drechslera monoceras capable of reducing both the amount of a chemical synthetic agrochemical used and the load on the environment by providing the Drechslera monoceras with selective herbicidal activities in a prescribed temperature region. SOLUTION: This Drechslera monoceras has selective herbicidal activities against Echinochloa sp. in a temperature region of 26-35 deg.C. The Drechslera monoceras is polymerase chain reactional(PCR) products obtained by carrying put the PCR reaction by using a DNA of the Drechslera monoceras as a template and a DNA having a base sequence represented by the formula as a primer. One of the PCR products has preferably 1.5-1.6kbp and the Drechslera monoceras is preferably MH-1901 (FERM BP-6091) or MH-2001 (FERM BP-6092).

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION
The present invention relates to Drechslera monoceras , which has herbicidal activity against Nobies in the temperature range above, and a weed controlling agent containing the fungus as an active ingredient.

[0002]

2. Description of the Related Art In recent years, environmental pollution by chemically synthesized pesticides has become a social problem. The development of herbicides using natural microorganisms is expected as one of new pesticides that does not pollute the environment. Several herbicides using live filamentous fungi have already been marketed. That is, DeVine (active ingredient: Phytophthora palmivola ) for controlling stranglevine, which is a weed of the potato family, and Colleg for controlling northern joint vetch, which is a weed of the legume family, in the United States.
o (active ingredient: Colletotrichum gloeosporioides ),
BioMal in Canada (active ingredient: Colletotrichum gl
oeosporioides f.sp. malvae ) and Lub in China
oaII (active ingredient: Colletotrichum gloeosporioides
f.sp.cuscutae ) is commercially available. In Japan,
To date, there is no pesticide registration of herbicides using live bacteria, but research on microbial herbicides targeting paddy weeds and lawn weeds has been conducted (Integrated Management of paddy and A).
quatic Weeds in Asia, FFTC Book Series No. 45, 199
Four). In particular, research on microbial herbicides has been actively carried out on nobies, which are highly harmful weeds of rice cultivation, and it has been found that Drexerrella spp., One of the plant pathogens, is effective in controlling Echinococcus spp. No. 219883,
It is described in JP-A-6-329513. Nevertheless, these studies have not yet been put to practical use.

Nobies are known for their wild species as weeds in rice fields around the world. Especially in Japan, it has long been a problem as a harmful weed in paddy fields. According to Yabuno (Research on Weeds, Vol. 2, 1975), nobies that are wild in the world are the species Echinochloa oryzic.
ola ), corona species ( E. colona ), pyramidalis species ( E. pyramidalis ), stagnina species ( E. stagnin)
a ), there are six species (species) of the genus Haploclada ( E. haploclada ) and the genus Cruzgari ( E. crus-galli ), and the Cruzgari species are three subspecies (variety), that is, formocensis ( E. . crus-galli var. formosensi
s ), Cruzgari ( E. crus-galli var. crus-galli ),
It is classified as Praticola ( E. crus-galli var. Pratycola ). Nobies, which have become a problem as a strongly harmful weed for rice cultivation, are considered to have been naturally crossed between these various species of Echinochloa or between their subspecies. A microbial herbicide targeted at controlling Nobies is required to have herbicidal activity against all species and all subspecies of the genus Echinochloa, and not exhibit pathogenicity in rice and other major crops. Furthermore,
Weeds of the genus Echinochloa germinate when the ambient temperature exceeds 10 ° C, and grow vigorously in a wide temperature range from 15 ° C to 35 ° C. Therefore, it is desired that a microbial herbicide that controls Nobies has a selective herbicidal action against the genus Echinochloa in a wide temperature range from a low temperature of about 15 ° C. to a high temperature of about 35 ° C.

As a filamentous fungus isolated from Echinochloa plants in the genus Dorekkusurera monoceras (Drechslera m
onoceras ) IFO-9619 and IFO-9800 are stored at the Fermentation Research Institute. However, as described later, these strains have no herbicidal effect on nobies, which are a highly damaging weed of rice cultivation.

As a Drexlera monoceras having herbicidal activity against nobies, Japanese Patent Laid-Open No. 4-2269 is disclosed.
MH-9011 (FERM BP-341)
6) is described. However, as described later, these strains have herbicidal activity in a normal temperature range of about 25 ° C., but have almost no herbicidal action on velvet in a temperature range exceeding 30 ° C.

On the other hand, in actual paddy fields and upland fields, a plurality of types of weeds are generated in addition to Nobies, and it is necessary to control weeds other than Nobies in rice cultivation. Currently, in order to control these weeds, it is common to use a combination of several herbicides with different control targets or use a mixture of multiple herbicides. Has become. Applying a large amount of chemically synthesized pesticides not only raises concerns about environmental pollution to water and soil, but also causes a vicious cycle of causing emergence of drug-resistant weeds, pests, and pathogenic bacteria. Therefore, development of new pesticides that are safe for humans and animals and do not pollute the environment is eagerly needed, and measures to reduce the amount of chemically synthesized pesticides are required.

[0007]

SUMMARY OF THE INVENTION An object of the present invention is to provide a microbial herbicide which is harmless to humans and animals and does not pollute the environment, eliminating the drawbacks of conventional weed control using only chemically synthesized pesticides. . Further, an object of the present invention is to provide a novel strain which exhibits a herbicidal action in a temperature range where the growth of Nobie is active, among Drexlera monoceras having selective herbicidal activity against Nobie, which is a highly damaging weed of rice cultivation. Another object of the present invention is to provide a weed controlling agent containing the viable cells as an active ingredient. It is a further object of the present invention to provide a method for controlling weeds, in which the fungus is mixed with an existing chemical herbicide to reduce the amount of the chemically synthesized pesticide and reduce the burden on the environment.

[0008]

Means for Solving the Problems In order to solve the above problems, the inventors of the present invention have searched for various plant pathogens existing in nature, and as a result, they have found practical use for Nobies in a temperature range where the growth of Nobies is active. A novel strain of Drexlera monoceras, which exhibits a natural herbicidal action and has no pathogenicity in rice and other major crops, was isolated from nature, and based on this, the present invention was achieved.

[0009] That is, the present invention provides a Drexlera monoceras having a selective herbicidal activity against Nobies (Echinochloa sp.) In a temperature range of 26 ° C to 35 ° C, and a weed control containing said Drexerella monoceras as an active ingredient. Agent.

[0010]

DESCRIPTION OF THE PREFERRED EMBODIMENTS The present invention will be described below in detail. The Dorexrela monoceras according to the present invention is a selective herbicidal activity test performed on tens of thousands of microorganisms that inhabit the natural world using weeds of the genus Echinochloa and rice (which shows pathogenicity to useful crops such as rice). Phytopathogenic fungi selected by No). The bacterium is not only at room temperature of about 25 ° C but also at a temperature in the range of more than 30 ° C,
It shows a practical herbicidal action against Nobies even at ℃.

[0011] The nobies to be controlled in the present invention are weeds classified into the genus Echinochloa ( Echinochloa sp.), And specifically, Echinochloa oryzicol.
a), swordfish ( Echinochloa crus-galli var. f
ormosensis ) and dog millet ( Echinochloa crus-galli va
r. crus-galli ). The Drexlera monoceras according to the present invention does not have any pathogenicity on grasses such as rice, barley, wheat, rye, oat, orchardgrass, Italian ryegrass, perennial ryegrass, which are main cultivated crops.

[0012] The strain of the present invention is characterized by a temperature of 26 ° C to 35 ° C.
In addition to having herbicidal activity against Nobies in the temperature range of
Two nucleotide sequences that hybridize with NA are 1.5k
bp to 1.6 kbp. As a typical strain of Drexlera monoceras according to the present invention, MH-1901 (FERM BP-6091)
And MH-2001 (FERM BP-6092). These strains have been deposited with the above deposit numbers at the Institute of Biotechnology and Industrial Technology of the Ministry of International Trade and Industry, 1-3-1 Higashi, Tsukuba, Ibaraki Prefecture, based on the Budapest Treaty on International Recognition of Deposits. I have.

[0013] The method of using Drexlera monoceras according to the present invention as a weed controlling agent may be a method using live cells obtained by culturing, a method using a filtrate obtained by culturing cells, or both. There is a way. As the viable cells, any of mycelia, conidia, or mycelia containing both of them can be used. A filamentous fungus belonging to the genus Dorexrela is established in a large-scale culture method as disclosed in JP-A-7-79784, and can be industrially used as a weed control agent.

In order to obtain a weed controlling agent using the Drexlera monoceras according to the present invention, the fungus may be used as it is, but usually, a viable bacterial cell such as a conidium is immiscible. It is desirable to mix it with an active carrier and form it into granules, powders, wettable powders, flowables, emulsions, liquids and the like. The carrier is usually used in agricultural and horticultural medicines, and may be any solid or liquid as long as it is biologically inactive, and is not limited to a specific one.
For example, as solid carriers, minerals such as clay, talc, kaolin, bentonite, white carbon, zeolite, silica, diatomaceous earth, corn flour, soybean flour, vegetable such as starch, polymers such as polyvinyl alcohol and polyalkyl Carriers. Examples of the liquid carrier include various organic solvents such as decane and dodecane, vegetable oil, mineral oil and water.

[0015] As an auxiliary agent, a stabilizer such as an antioxidant and a pH adjuster, as well as a surfactant and a binder, can be used if necessary.

The application rate of Drexlera monoceras in the weed controlling agent according to the present invention varies depending on the form of preparation and the treatment method, but as conidia, 1 × 1 per round.
0 ⁶ to 1 × 10 ¹² , preferably 1 × 10 ⁸ to 1 × 10 ¹⁰
Individual.

The weed controlling agent of Drexlera monoceras according to the present invention is mixed with a chemical herbicide,
A sufficient control effect can be obtained at an application rate that cannot be controlled by itself. Therefore, the fungus not only contributes to crop production, but also can reduce the amount of chemical herbicides used, and is useful for reducing environmental pollution.

The weed controlling agent for Drexlera monoceras according to the present invention is a chemically synthesized pesticide for Nobies,
For example, when mixed with a chemical herbicide such as a diphenyl ether-based, amide-based or carbamate-based chemical herbicide, a synergistic control effect can be obtained as compared with the case where each is used alone. Also,
Chemical herbicides intended for broadleaf weeds, such as diazines, sulfonylureas, and triazines, when mixed with chemical herbicides, not only enables the control of broadleaf weeds, but also the control of broadleaf weeds, as well as Drexlera monoceras. The application rate can be reduced. Regarding its mechanism of action, the uptake and translocation of chemical herbicides was increased due to plant tissue damage caused by the infection of Drexlera monoceras according to the present invention, and similarly, the herbicides were damaged. It is presumed that the infection and propagation of Drexlera monoceras in plant tissues are facilitated, and that the herbicidal action is enhanced.

Examples of the chemical herbicides that can be used include the following herbicides. 2,4-D, MCPB, phe
nothiol, naproanilide, prop
anil, ethobenzanide, buromo
butide, mefenacet, butachlo
r, metolaclor, pretilachlo
r, thenylchlor, diflufenica
n, thiobencarb, molinate, di
meperate, esprocarb, pyri
buticarb, karbutilate, asul
am, diuron, linuron, dymron,
cumyluron, bensulfuron-met
hyl, pyrazosulfuron-ethyl,
cinosulfuron, imazosulfuro
n, azimsulfron, cyclosulfam
uron, butamifos, piperopho
s, anilofos, trifluralin, pe
ndimethalin, quinoclamine,
quinchlorac, dichrobenil, i
oxynil, sethoxydim, sulcotr
ione, befenox, acifluorphen
e-sodium, fluazifop-butyl,
quizalofop-ethyl, cyhalofo
p-butyl, benfuresate, cinme
thylin, norflurazon, isoxaf
lutole, bentazone, bromaci
l, pyrazolate, pyrazoxyfen,
Benzofenap, imazapyr, imaza
quin, cafenstrole, pentoxaz
one, pyriminobac-methyl, bi
spyribac-sodium, simazine,
atrazine, symmetry, prometr
yn, dimethametryn.

The weed controlling agent of Drexlera monoceras according to the present invention may be separately used in combination with an existing chemical herbicide, but a chemical herbicide which does not inhibit conidia germination or hyphal elongation of the fungus, for example, mefenacet. And naproa
As for nilide and the like, a mixed composition can be prepared and used. The content of Drexlera monoceras related to the weed control agent of the present invention varies depending on the form of preparation, but in solid preparations such as granules and wettable powders, 1 × per gram is used.
It is 10 ⁵ to 1 × 10 ⁸ , preferably 1 × 10 ⁶ to 1 × 10 ⁷ .

[0021]

EXAMPLES Hereinafter, the present invention will be described in more detail with reference to the examples of the present invention, and the control of weeds containing the fungus as an active ingredient, but the present invention is not limited to these examples.

[Example 1] Isolation of Drexlera monoceras according to the present invention A naturally-occurring Nobie was collected, and pathogenic bacteria were isolated from diseased tissues of the leaf blade and leaf sheath. That is, a 1 cm square tissue section centering on the lesion is prepared, immersed in a 70% ethyl alcohol aqueous solution for 1 to 2 seconds, and then placed in an aqueous solution of sodium hypochlorite having an effective chlorine concentration of about 2% for 10 minutes. The surface of the section was sterilized by immersion. A section of the diseased tissue whose surface had been sterilized was washed three times with sterile water, then placed on an agar medium to which an antibiotic was added, and cultured statically in an incubator. The tips of the growing hyphae were sequentially picked under a stereoscopic microscope, transplanted into a nutrient medium, and pure culture was performed.

Test Example 1 Assay and Identification of Selective Herbicidal Activity of Isolated Filamentous Fungi (1) Assay of Selective Herbicidal Activity Weeds and rice varieties of the genus Echinochloa were aseptically grown in test tubes. The test material was used. In other words, as a weed of the genus Echinochloa, Echinochloa oryzicola ,
Black-finned millet ( E. crus-galli var. Formosensis ),
The seeds of dog millet ( E. crus-galli var. Crus-galli ) and rice, Nipponbare, Koshihikari, and Sasanishiki were surface-sterilized by the above-described method, and were seeded and grown in plant test tubes containing sterile distilled water. On the other hand, purely isolated filamentous fungi
Plate culture was performed at 25 ° C. for 1 week on a potato dextrose agar medium (manufactured by Difco), and a microflora disk prepared by punching out the outer edge of the microflora with sterilized cork borer was used as an inoculation source. The microflora discs were inoculated into test tubes in which weeds or rice of the genus Echinochloa were grown, and cultured in a chamber for growing plants. Two weeks after inoculation, the effect of the test bacteria on Echinochloa weeds and rice was determined by
(No effect), +, ++, +++ (withering) were evaluated using a four-point index. The results are shown in Table 1-1 (Table 1) and Table 1-2.
It is shown in (Table 2).

[0024]

[Table 1] Table 1-1. Herbicidal activity of the filamentous fungus according to the present invention against Echinochloa weeds.ヌ イ IFO-9619 *---IFO-9800 *---MH -9011 * ++++++ MH-1901 ++++++++++ MH-2001 ++++++++++ ─────── *: Known strain

[0025]

Table 1 Table 2 Pathogenicity of the filamentous fungus according to the present invention to riceニ Nipponbare Koshihikari Sasanishiki ＩＦ IFO-9619 * ---IFO-9800 *---MH-9011 *---MH-1901---MH-2001---Non-contact---─────────────── ───────────────────── *: Known strain

(2) Identification of Filamentous Fungi Filamentous fungi having remarkable herbicidal activity against Echinococcus weeds and having no effect on rice were identified. As a result, MH-1901 and MH-2
In the case of 001, the colonies on the malt agar medium (manufactured by Difco) were gray-black and showed irregular growth. The conidium is clearly marked with hilum, and the size is 87.5-12.
7.5 μm × 15-17.5 μm, its shape is slightly bent. The maximum number of the partition walls is 9, and most of the partition walls are 5 to 7. The conidium pattern is straight. Based on the above characteristics, Ellis classification (Ellis, MB (1971) Demariaceus Hyp
homycetes, Commonwealth Mycological Institute, Ke
w, England and Ellis, MB (1976) More Demariaceus
Hyphomycetes, Commonwealth Mycological Institute,
Kew, England), the filamentous fungi MH-1901 and M
H-2001 none of Dorekkusurera monoceras (Dr
echslera monoceras ). These new strains are
MH-1901 is FERM BP-6091, MH-2
001 has been deposited as FERMBP-6092 with the Institute of Biotechnology and Industrial Technology, respectively.

[Test Example 2] Identification of the strain of Drexlera monoceras according to the present invention from known strains Drexerella monoceras MH-19 according to the present invention
01, MH-2001 and the known Drexlera monoceras MH-9011 (Accession No. FERM BP-34)
No.16, the RAPD method (Random A) was applied to the Institute of Biotechnology and Industrial Technology, respectively, based on the Budapest Treaty on International Recognition of Deposits.
mplyfied Polymorphic DNA, Williams, JGK et al (1
990) DNApolymorphisms amplified by arbitrary prime
rs are useful as genetic markers, Nucl. Acids Res.
vol.18), the electrophoretic patterns of the PCR products obtained were compared. MH-1901, MH-2001 and MH-
DNA was extracted and purified from the cells of 9011 according to a conventional method. The purified DNA and a commercially available PCR reagent (manufactured by Takara Shuzo) were mixed at the ratio shown in Table 2 (Table 3) to obtain a PCR reaction solution.

[0028]

[Table 3] Table 2. Composition of PCR reaction solution ───────────────────── PCR buffer (10 times) 1.5 μl 25 mM magnesium chloride 0.3 μl 2.5 mM dNTP 0.6 μl Taq polymerase 0.06 μl 0.1 μM primer 0.6 μl DNA (1 ng / μl) 0.5 μl sterile water 11.34 μl ─────────────────────

The primer was OPE-1 (5'-CCCAAGGTCC-) for RAPD manufactured by Operon Technology.
3 ′) was used. The PCR reaction was performed using a thermal cycler (Perkin Elmer, Gene Amp PCR System 960).
Using (0), the reaction conditions are 94 ° C. and 2 minutes to (94
° C, 10 seconds → 40 ° C, 1 minute → 72 ° C, 1 minute) × 4
The test was performed 5 times at 72 ° C. for 10 minutes. The DNA obtained by the PCR reaction was subjected to submarine electrophoresis (50 V, 50 minutes) on a 1.5% agarose gel, and then subjected to 0.
Staining was performed by immersion in a 5 μg / ml ethidium bromide aqueous solution for 20 minutes, and the band pattern of the PCR product was examined under ultraviolet irradiation. Fig. 1 (Fig. 1)
Shown in Electrophoretic pattern of MH-9011 PCR product and MH-1901 and MH-2001 according to the present invention
When compared with the electrophoresis pattern of the PCR product of the above, both are clearly different.

[Test Example 3] Herbicidal activity by temperature of Drexlera monoceras according to the present invention Drexlera monoceras MH-19 according to the present invention
01, MH-2001 and a known strain MH-9011 were inoculated on an automill agar medium (manufactured by Difco), and 25
Static culture was performed at 2 ° C. for 2 weeks. The conidia formed on the surface of the colony were treated with 0.02% Triton X-100.
Suspended in an aqueous solution (manufactured by Rohm and Haas)
A weed controlling liquid (1 × 10 ⁵ conidia / ml) containing Drexrela monoceras as an active ingredient was prepared. Meanwhile, 1
Fifty seeds of Nobie were sowed in paddy soil packed in a pot of 1 / 10,000 are and grown until the first leaf stage. Water was added to the pot where the seedlings were raised to a depth of about 5 cm. 1 ml of the above-mentioned weed controlling agent was dropped into this pot. 10 ° C, 20 ° C,
Nobies were cultivated in a climate chamber (illuminance: 10,000 lux, light / dark period; 12/12 hours) set at 25 ° C., 30 ° C. and 40 ° C., and after 2 weeks of treatment with a weed controlling liquid, remaining individuals and mortality Individuals were counted and the control rate against Nobies was calculated. The test was performed in triplicate, and the average control rate was 3rd.
The results are shown in Table (Table 4).

[0031]

[Table 4] Table 3. Herbicidal activity by temperature of Drexlera monoceras according to the present invention ───────────────────────────────── Set temperature (° C) 10 20 25 26 28 30 35 40 ───────────────────────────────── MH-9011 * − ** 76 98 90 70 4 2 − *** MH-1901 − ** 100 100 100 100 100 70 − *** MH-2001 − ** 100 100 100 100 100 70 − *** No inoculation − ** 0 0 0 0 0 0 − *** ───────────────────────────────── *: Known strain **: Nobie due to low temperature Does not grow ***: Almost nobies die due to high temperature

Formulation Example 1 Conidium 1 of wettable powder Drexlera monoceras MH-1901
90 g of Toalite (manufactured by Toa Kasei Co., Ltd.) was thoroughly mixed with 90 g of the suspension containing × 10 ⁹ , and air-dried sufficiently at room temperature, followed by crushing. The crushed product and Newcalgen (manufactured by Takemoto Yushi Co., Ltd.) were kneaded and sieved to obtain a wettable powder of Drexlera monoceras according to the present invention.

[Formulation Example 2] Dust Conidium 1 of Drexlera monoceras MH-2001
90 g of Toalite (manufactured by Toa Kasei Co., Ltd.) was thoroughly mixed with a suspension containing × 10 ⁹ and air-dried sufficiently at room temperature. This was crushed and then sieved to obtain a powder of Drexlera monoceras according to the present invention.

Formulation Example 3 Granules Conidium 1 of Drexlera monoceras MH-1901
After thoroughly mixing 88 g of Toalite and 2 g of cellogen (Daiichi Kogyo Seiyaku Co., Ltd.) with a suspension containing × 10 ^{9, the} mixture is granulated, sieved, and air-dried by a small-sized extrusion granulator according to the present invention. A granule of Drexlera monoceras was obtained.

[Formulation Example 4] Granules mixed with a chemical herbicide Emulon granules finely pulverized (manufactured by Sankyo, CN as an active ingredient)
After mixing well 10% by weight of P) and 78 g of zeolite (manufactured by Sanyo Kokusaku Pulp Co.), a suspension containing 1 × 10 ⁸ conidia of Drexlera monoceras was added and wetted. Was. Cellogen (Daiichi Kogyo Pharmaceutical) 2
g, mixed well, and then granulated, sieved, and air-dried with a small extrusion granulator to obtain a mixed granule of Drexlera monoceras and CNP according to the present invention.

[Test Example 4] Mixed test of Drexlera monoceras according to the present invention and a chemical herbicide for Nobies Approximately 100 seeds of Nobies were sowed in paddy soil filled in a quantitative pot of 1/1000 are. And Nobies were raised until the first leaf stage. Water was poured into this pot to make the depth of flooding about 5 cm. A wettable powder prepared according to Formulation Example 1 is treated, and a chemical herbicide for nobies, hinokloa granule (active ingredient: m
efenacet). 30 ° C artificial weather chamber (illuminance; 10,000 lux, light / dark period; 12/12
Nobies were cultivated for 1 hour after the treatment, and the remaining individuals were counted one month after the treatment, and the control rate against the Nobies was calculated by the following equation. The test was performed in triplicate, and the average control rate is shown in Table 4 (Table 5).

[0037]

[Table 5] Table 4. Herbicidal effect (control rate%) by mixing Drexlera monoceras according to the present invention with a chemical herbicide for Nobies ─Hinochlore granule (mg) Conidia 300 100 30 100 ─────────────────────────── 1 × 10 ⁶ 100 100 100 100 100 3 × 10 ⁵ 100 100 100 100 77 1 × 10 ⁵ 100 100 100 78 51 3 × 10 ⁴ 100 100 77 53 24 0 100 74 52 22-──────────────── ───────────

[Test Example 5] Mixed test of Drexlera monoceras and broadleaf chemical herbicide according to the present invention Approximately 100 thousand nobies seeds and 1 / 1,000 ares of broadleaf weeds such as conger and spoonbrush The seedlings were sown in paddy field soil packed in a sex pot, and seedlings were grown until the leaf flies reached the single leaf stage. Water was placed in the pot where the seedlings were raised to a depth of about 5 cm. A wettable powder prepared according to Formulation Example 1 was treated, and Sirius granules (active ingredient: pyrazosulf)
ron-ethyl) 300 mg was processed. 30 ° C artificial climate chamber (illuminance: 10,000 lux, light / dark period;
12/12 hours), the remaining individuals were counted one month after the treatment, and the control rate against Nobies was calculated by the formula of Test Example 4. The test was performed in triplicate, and the average control rate is shown in Table 5 (Table 6). In addition, in all of the 300 mg Sirius granule-treated groups, the control rate of the corn and the Japanese sprout was 100%.

[0039]

[Table 6]

[0040]

EFFECTS OF THE INVENTION The Drexlera monoceras according to the present invention has a herbicidal activity against all kinds of nobies (Echinochloa), which are weeds, not only at room temperature but also at a temperature range of 26 to 35 ° C. A controlling effect is obtained. on the other hand,
It has no pathogenic effects on rice and other major crops, and exhibits a high degree of selectivity, which is difficult with chemical herbicides. Furthermore, the use of the mixture of Drexlera monoceras and the chemical herbicide according to the present invention, or the use of such a mixture, enables a reduction in the amount of the drug necessary for the control of Nobies, resulting in environmental pollution and drug-resistant weeds that have recently become apparent. It also helps to avoid appearance.

[0041]

[Sequence list]

SEQ ID NO: 1 Sequence length: 10 Sequence type: Nucleic acid Topology: Linear Sequence: CCCAAGGTCC 10

[Brief description of the drawings]

FIG. 1 is a diagram showing an electrophoresis pattern of a PCR product.

[Description of Signs] kpb: kilobase pair λHindIII: λDNA cut with restriction enzyme HindIII

 ──────────────────────────────────────────────────続 き Continued on the front page (72) Inventor Satsuki Hirase, 1144 Togo, Togo, Mobara-shi, Chiba Mitsui Chemicals Co., Ltd. (72) Inventor Takeshi Nakamura 1144, Togo, Togo, Mobara-shi, Chiba Mitsui Chemicals, Inc. (72) Inventor Makoto Nishida 1144 Togo, Mobara-shi, Chiba Mitsui Chemicals, Inc.

Claims (11)

[Claims] 1. In a temperature range of 26 ° C. to 35 ° C., nobies (Ec
hinochloa sp.), which has selective herbicidal activity. 2. The DN of said Drexlera monoceras
One of the PCR products obtained by performing a PCR reaction using A as a template and a DNA having the base sequence of SEQ ID NO: 1 in the sequence listing as a primer is 1.5 kbp to 1.
2. The Drexlera monoceras according to claim 1, wherein the molecular weight is 6 kbp. 3. The Drexlera Monoceras has MH-1
901 (FERMBP-6091). 4. Drexlera Monoceras has MH-2
001 (FERMBP-6092). 5. The drex reeler according to claim 1,
Monoceras conidia. 6. A weed controlling agent comprising the Drexlera monoceras according to claim 1 as an active ingredient. 7. The DN of the Drexlera monoceras
One of the PCR products obtained by performing a PCR reaction using A as a template and a DNA having the nucleotide sequence of SEQ ID NO: 1 in the sequence listing as a primer is 1.5 kbp to 1.6 kbp.
The weed controlling agent according to claim 6, which is kbp. 8. The system according to claim 1, wherein said Drexrella monoceras is MH-1.
901 (FERMBP-6091). 9. The Drexlera Monoceras has MH-2
001 (FERMBP-6092). 10. The weed controlling agent according to claim 6, comprising a chemical herbicide as an active ingredient. 11. The herbicide according to claim 1, wherein the chemical herbicide is at least one selected from the group consisting of a diphenyl ether herbicide, an amide herbicide, a carbamate herbicide, a diazine herbicide, a sulfonylurea herbicide and a triazine herbicide. The weed controlling agent according to claim 10, which is an agent.