JPH10150972A - Container for shaking culture - Google Patents
Container for shaking cultureInfo
- Publication number
- JPH10150972A JPH10150972A JP30835896A JP30835896A JPH10150972A JP H10150972 A JPH10150972 A JP H10150972A JP 30835896 A JP30835896 A JP 30835896A JP 30835896 A JP30835896 A JP 30835896A JP H10150972 A JPH10150972 A JP H10150972A
- Authority
- JP
- Japan
- Prior art keywords
- container
- culture
- length
- gusset bag
- shaking culture
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/14—Bags
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/20—Material Coatings
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/16—Vibrating; Shaking; Tilting
Landscapes
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Clinical Laboratory Science (AREA)
- Immunology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
Description
【０００１】[0001]
【発明の属する技術分野】本発明は注出口部材を有する
振盪培養用の容器に関し、さらに詳しくは、容器の内外
部または培養液と容器内気体とのガス交換能が優れ培養
効率がよく、作業性の良好な振盪培養容器に関する。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a container for shaking culture having a spout member, and more particularly to a container having excellent gas exchange ability between the inside and outside of a container or between a culture solution and a gas in the container, having a high culture efficiency and a high working efficiency. The present invention relates to a well-cultured shaking culture vessel.
【０００２】[0002]
【従来技術】従来より微生物、植物組織の振盪培養を行
うに際しては、培養液中に細胞（組織）を入れ振盪させ
て気体と培養液との接触を良くして行っている。そし
て、この培養を行う容器としては、三角フラスコ、試験
管、ビーカー等のガラス容器や硬質プラスチック製容器
が使用されている。しかし、ガラス容器は破損の恐れが
あり、また、これらの容器を反復使用する場合、菌の混
ざり合いを防止するため十分な洗滌或は殺菌を行う必要
があったが、この洗滌操作は手間がかかり、殊に試験管
や三角フラスコのような開口部の狭い容器の場合は洗浄
ばかりではなく、内容物の出し入れが不便であった。そ
こで、本発明者らは先にこの点を改良して作業性の良好
な植物培養用容器を提案した（実開平３−１２６８５０
号公報参照）。この容器は、図４に示すように、内面に
熱接着性合成樹脂層を有する２枚のシート状の表裏面材
３、４及びこれらの表裏面材３、４の間にあって、それ
ぞれの下縁部に接続した内方につつ折り状にした底面材
５とからなり、前記表裏面材の少なくとも一方に透孔１
１を設け、この透孔にはフィルターを貼着し、底面材５
を開くと図４に示したような形状をし、使用に際しては
上方の開口部を封口治具１２によって封じていた。この
容器により先に述べた作業性は大いに改善されたが、こ
の容器は、未使用時には底面材がつつ折り状になって折
り畳んであるため使用時には底面を開いて容器を形成し
なければならず、また、容器の上方になるにしたがって
表面材３と裏面材４との間隔がせばまり、開口部８は封
口治具１２によって封じられているので容器内の空間が
狭く、培養に必要な換気は単に面材に設けた透孔１１に
よって行われるだけであるため培養細胞が縊死したり、
或は培養代謝ガスの発生のため容器が膨張し破袋を生ず
るおそれがあった。2. Description of the Related Art Conventionally, when shaking culture of a microorganism or plant tissue is performed, cells (tissue) are placed in a culture solution and shaken to improve the contact between gas and the culture solution. As a container for performing this culture, a glass container such as an Erlenmeyer flask, a test tube, or a beaker or a hard plastic container is used. However, glass containers may be damaged, and when these containers are used repeatedly, it is necessary to perform sufficient washing or sterilization to prevent mixing of bacteria, but this washing operation is troublesome. In particular, in the case of a container having a narrow opening such as a test tube or an Erlenmeyer flask, not only washing but also taking out and in of the contents is inconvenient. Therefore, the present inventors have previously improved this point and proposed a container for plant culture having good workability (Japanese Utility Model Application Laid-Open No. 3-126850).
Reference). As shown in FIG. 4, the container has two sheet-like front and back members 3 and 4 having a heat-adhesive synthetic resin layer on the inner surface and a lower edge between the front and back members 3 and 4. And a bottom material 5 folded inward while being connected to the portion, and a through hole 1 is formed in at least one of the front and back materials.
1, a filter is attached to the through hole, and a bottom material 5
When it was opened, it was shaped as shown in FIG. 4, and the upper opening was closed with a closing jig 12 in use. Although the above-described workability has been greatly improved by this container, the bottom of the container is folded and folded when not in use, so the bottom must be opened to form the container when in use. Further, the distance between the front surface material 3 and the back surface material 4 is narrowed toward the upper part of the container, and the opening 8 is closed by the closing jig 12, so that the space in the container is narrow, which is necessary for culture. Ventilation is simply performed by the through holes 11 provided in the face material, so that the cultured cells may be hung or
Alternatively, the container may expand due to the generation of culture metabolic gas, and the bag may be broken.
【０００３】しかして、振盪培養用容器としては容器内
の空間が広く、培養に必要な換気が十分に行えるように
培養液を攪拌して円流を生じさせることが好ましい。そ
のためには、容器の形状としては底面が正方形状の直方
体が好ましいのであるが、底面が正方形状をしたガゼッ
ト容器の場合、ガゼットの襞部分が袋の中央部まで食込
むので、培養液を注入や注出を行うための注出口部材が
取付けにくく、また、一辺を開口したオープンマウス袋
の場合は培養液の出し入れ、植菌等の取扱いは容易であ
るが、雑菌が混入するおそれがある。[0003] However, as a container for shaking culture, it is preferable that the space inside the container is large and the culture solution is stirred to generate a circular flow so that sufficient ventilation required for culture can be performed. For this purpose, the shape of the container is preferably a rectangular parallelepiped with a square bottom, but in the case of a gusset container with a square bottom, the culture solution is injected because the gusset folds up to the center of the bag. In the case of an open mouth bag with an open side, it is easy to take in and out of the culture solution and inoculate the cells, but there is a possibility that various bacteria may be mixed.
【０００４】[0004]
【発明が解決しようとする課題】そこで、本発明者は上
記の欠点を改良し、培養液の攪拌を容易にして十分換気
を行えるようにすると共に、容器内の容積を広くし、容
器内の気体と培養液内の気体とのガス交換が十分行える
ような容器について種々検討した結果、本発明を完成し
たもので、本発明の目的は、容易に培養液を攪拌するこ
とが出来、培養液と容器内気体とのガスとのガス交換能
にすぐれた培養効率のよく、作業性の良好な振盪培養容
器を提供することである。Accordingly, the present inventor has improved the above-mentioned drawbacks, facilitated the stirring of the culture solution to allow sufficient ventilation, increased the volume in the container, and increased the volume in the container. As a result of various studies on a container capable of sufficiently performing gas exchange between gas and gas in the culture solution, the present invention has been completed, and an object of the present invention is to enable easy stirring of the culture solution, It is an object of the present invention to provide a shaking culture container having excellent culturing efficiency and excellent workability, which is excellent in gas exchange ability between gas and gas in a container.
【０００５】[0005]
【課題を解決するための手段】本発明の要旨は、内層に
熱接着性合成樹脂層を有する包装材で構成され、開口部
に注出口部材を突設したガゼット袋よりなる振盪培養用
容器であって、該ガゼット袋の開口部の長さが、ガゼッ
ト袋の襞部の長さと前記注出口部材の基部の長さの和に
等しく、且つ、該ガゼット袋の上部に少なくとも１か所
に貫通孔を穿設して、該部分にフィルター材料を貼着し
たことを特徴とする振盪培養用容器である。即ち、本発
明に係る培養容器おいては、容器の底面より上方の開口
部に到るまでの表面と裏面及び両側壁面は同形の直方体
であるため容器内の空間は広がり、また、ガゼット袋の
開口部の長さが襞部の長さと前記注出口部材の熱接着部
の長さの和に等しいので、この容器の底面の形状はほぼ
正四方形の形状に近く、従って、撹拌時の培養液の流れ
を円流に近い流れとすることができるので振盪培養効率
を向上させることができると共に、開口部には注出口部
材を突設してあるので、培養液の出し入れや植菌等の操
作が容易に行なえるのである。更に、フィルター材料の
みによる換気では不十分は場合には開口部に突設した注
出口部材に例えば特公昭５９−３４５８５号に示されて
いるような弁を取付けることが好ましく、かかる弁を設
けることによって、容器内の換気を十分行うことがで
き、また、培養中に外部からの雑菌の混入を防止するこ
とが出来る。The gist of the present invention is a container for shaking culture comprising a gusset bag which is constituted by a packaging material having a heat-adhesive synthetic resin layer as an inner layer and a spout member is protruded at an opening. The length of the opening portion of the gusset bag is equal to the sum of the length of the fold portion of the gusset bag and the length of the base of the spout member, and penetrates at least one location above the gusset bag. A container for shaking culture, characterized in that a hole is formed and a filter material is adhered to the portion. That is, in the culture container according to the present invention, since the front surface, the back surface, and both side walls up to the opening above the bottom surface of the container are the same rectangular parallelepiped, the space in the container is expanded, and the gusset bag is Since the length of the opening is equal to the sum of the length of the fold and the length of the heat-bonded portion of the spout member, the shape of the bottom surface of the container is almost a square shape, and therefore, the culture solution at the time of agitation. Flow can be made to be a flow close to a circular flow, so that shaking culture efficiency can be improved, and a spout member is provided at the opening, so operations such as taking in and out of the culture solution and inoculation can be performed. Can be done easily. Further, if ventilation with only the filter material is not sufficient, it is preferable to attach a valve as shown in Japanese Patent Publication No. 59-34585 to a spout member protruding from the opening, for example. This makes it possible to sufficiently ventilate the inside of the container, and to prevent contamination of bacteria from the outside during culture.
【０００６】[0006]
【発明の実施の態様】本発明について更に詳細に説明す
る。本発明において使用する内層に熱接着性合成樹脂層
を有する包装材とは一方の面に熱融着性合成樹脂層を有
する耐熱性及び耐水性のある合成樹脂シートであって、
例えばポリエチレンまたはポリプロピレンなどのポリオ
レフィン樹脂、ポリエステル樹脂、ポリアミド樹脂など
の耐熱性及び耐水性を有する合成樹脂シートの一方の面
にポリエチレンなどの熱接着性樹脂層を積層したもの
で、熱接着性樹脂層を内面として培養用容器が形成され
ている。BEST MODE FOR CARRYING OUT THE INVENTION The present invention will be described in more detail. The packaging material having a heat-adhesive synthetic resin layer in the inner layer used in the present invention is a heat- and water-resistant synthetic resin sheet having a heat-fusible synthetic resin layer on one surface,
For example, a heat- and water-resistant synthetic resin sheet such as a polyolefin resin such as polyethylene or polypropylene, a polyester resin, or a polyamide resin is laminated with a heat-adhesive resin layer such as polyethylene on one surface. Is formed as an inner surface to form a culture vessel.
【０００７】本発明では上記の包装材をもってガゼット
袋を構成するのであるが、開口部に注出口部材を突設す
るため、ガゼット袋の襞部の長さと注出口部材の熱接着
部分の長さの和が開口部の長さにほぼ等しくする。開口
部の長さ、襞部の長さ及び注出口部材の熱接着部分の長
さの三者をこのような関係にすることによって、開底し
た容器の底面の形状はほぼ正方形に近い形状とすること
ができる。本発明で使用する注出口部材としては、特に
限定は無く、通常の包装容器に取り付けられている注出
口部材で良く、例えば、熱接着性樹脂を成形したもの
で、形状としては舟型をした基部の中央部に、注出口を
形成する垂直に管を突設したものが使用できる。そし
て、突設した管の口部には綿をつめて外部からの雑菌の
混入を防止する。或いは、注出口に容器の内部より外部
へのみの換気が行なえる弁を取付けても良く、例えば特
公昭５９−３４５８５号公報に記載されているような弁
を使用することによって、培養中外部からの雑菌の混入
を防止することが出来る。注出口部材を容器に取り付け
るに際しては、注出口部材の基部を表面材と後面材との
間に挟持し、両面材と熱圧着して接着しても良いが、再
使用ができるようにクリップ等を用いて挟着しても良
い。容器に取り付けた注出口部材の注出口にはスポンジ
等の多孔体で通気性を保持しながら外部からのごみ、雑
菌等の侵入を防止する。In the present invention, the gusset bag is constituted by the above-mentioned wrapping material. However, since the spout member protrudes from the opening, the length of the fold portion of the gusset bag and the length of the heat bonding portion of the spout member are set. Is approximately equal to the length of the opening. By making the length of the opening, the length of the fold, and the length of the heat-bonded portion of the spout member such a relationship, the shape of the bottom surface of the opened container is substantially a square shape. can do. The spout member used in the present invention is not particularly limited, and may be a spout member attached to a usual packaging container.For example, a spout member formed by molding a thermo-adhesive resin and having a boat shape is used. A vertically protruding tube forming a spout at the center of the base can be used. Then, the mouth of the protruding pipe is filled with cotton to prevent contamination of various bacteria from the outside. Alternatively, a valve capable of ventilating only from the inside of the container to the outside may be attached to the spout. Of various bacteria can be prevented. When attaching the spout member to the container, the base of the spout member may be sandwiched between the surface material and the back surface material, and may be bonded by thermocompression bonding with the both surface materials. You may pinch using. The spout of the spout member attached to the container keeps air permeability with a porous material such as sponge, and prevents invasion of dust, germs and the like from the outside.
【０００８】本発明の培養用容器の表面材又は裏面材の
少なくとも一方の面材の上部に少なくとも１か所貫通孔
を穿設し、この貫通孔の内面又は外面から通気性を有す
るが菌の混入を防止するフィルターを貼着する。貫通孔
の大きさとしては、容器の大きさによって適宜に決定す
れば良いが、通常タテ約３０ｍｍ程度、ヨコ約６０ｍｍ
程度であり、また、フィルターとしてはポリエステル系
繊維、ポリエチレン系繊維、ポリプロピレン系繊維等か
らなる不織布を使用する。フィルターの貼着手段として
は熱融着や接着剤等を使用する。本発明の培養用容器の
内容積（培養液の容積）としては、２００ｍｌ〜２００
０ｍｌ程度として、振盪培養効率を考慮して、底面の形
状としては長辺が１００〜２５０ｍｍ、短辺５０〜１５
０ｍｍ、高さ１５０〜３００ｍｍ程度が良く、ガゼット
の襞のない部分を、即ち、ガゼット袋の表面材と裏面材
の２枚の部分の幅を３０〜５０ｍｍ程度あけて、この部
分に注出口部材の基部を取り付ける。注出部を形成する
突設部の長さは、特に制限はない培養液、植菌等に不都
合のない長さ、１０〜５０ｍｍ程度で注出口の内径は１
０〜２０ｍｍ程度である。[0008] At least one through-hole is formed in an upper portion of at least one of the front and back surfaces of the culture vessel of the present invention, and the inside or the outside of the through-hole has air permeability but does not contain bacteria. Attach a filter to prevent contamination. The size of the through-hole may be appropriately determined according to the size of the container, but is usually about 30 mm in length and about 60 mm in width.
The filter is made of a nonwoven fabric made of polyester fiber, polyethylene fiber, polypropylene fiber, or the like. As a means for attaching the filter, heat fusion, an adhesive or the like is used. The inner volume (volume of culture solution) of the culture container of the present invention is 200 ml to 200 ml.
Considering the shaking culture efficiency, about 0 ml, the shape of the bottom surface is 100-250 mm on the long side and 50-15 mm on the short side.
0 mm and a height of about 150 to 300 mm are good, and there is no gusset fold, that is, the width of the two parts of the surface material and the back material of the gusset bag is about 30 to 50 mm, and the spout member is provided in this part. Attach the base. The length of the projecting portion forming the pouring portion is not particularly limited, and is a length that is not inconvenient for the culture solution, inoculation, etc., is about 10 to 50 mm, and the inner diameter of the pouring port is 1
It is about 0 to 20 mm.
【０００９】次に図をもって本発明を説明する。図１は
本発明にかかる振盪培養用容器の斜視図であり、図２は
その容器を折り畳たんだ状態の平面図であり、図４は先
に提案した培養容器である。図において、容器１を構成
する包装材２は、振盪培養用容器のガゼット包装袋を構
成するように、二つ折りにした側面材５を表面材３と裏
面材４との間に介在させ、側縁部６及び底辺部７を融着
する。上部の開口部には表面材３と裏面材４との間に注
出口部材９の基部を挟み熱圧着して上部融着部８を形成
して注出口部材９を接着し、下部はガゼット袋の底辺７
と襞部の折り込み線１０との交点より側縁部６に対して
約４５度の角度をもって融着し、開底した場合、底面を
構成するようにする。そして、本発明においては、上下
辺の長さは側縁部６から襞部の折り込み線１０までの長
さａ及び注出口部材の基部に長さｂにほぼ等しい。また
表面材３及び裏面材４の何れか一方に貫通孔１１を設
け、これにフィルターを貼着する。このような振盪培養
用容器の注出口部材の注出口から培養液を注入すると、
容器は直ちに開底して図１の状態になり、底面はほぼ正
方形に近似した形状となり、撹拌すると円流を生じる。Next, the present invention will be described with reference to the drawings. FIG. 1 is a perspective view of a container for shaking culture according to the present invention, FIG. 2 is a plan view of the container in a state where the container is folded, and FIG. 4 is a culture container proposed earlier. In the figure, a packaging material 2 constituting a container 1 has a folded side material 5 interposed between a front material 3 and a back material 4 so as to constitute a gusset packaging bag of a container for shaking culture. The edge 6 and the bottom 7 are fused. In the upper opening, the base of the spout member 9 is sandwiched between the surface material 3 and the back material 4 and thermocompression-bonded to form an upper fused portion 8 and the spout member 9 is adhered, and the lower portion is a gusset bag. Base 7 of
Is welded to the side edge 6 at an angle of about 45 degrees from the intersection of the fold line and the folding line 10, and when the bottom is opened, a bottom surface is formed. In the present invention, the length of the upper and lower sides is substantially equal to the length a from the side edge 6 to the folding line 10 of the fold and the length b at the base of the spout member. Further, a through hole 11 is provided in one of the front surface member 3 and the back surface member 4, and a filter is attached to this. When a culture solution is injected from the spout of the spout member of such a shaking culture vessel,
The container is immediately opened to the state shown in FIG. 1, and the bottom surface has a shape substantially similar to a square, and when stirred, a circular flow is generated.
【００１０】[0010]
実施例１ 幅１３０ｍｍ、奥行き１００ｍｍの底面で高さが１６０
ｍｍの直方体の開口部に内径１５ｍｍの注出口部材を熱
接着により取り付けた。そして、前面及び後面の２か所
に横６０ｍｍ，縦３０ｍｍの透孔を設け、この透孔にポ
アサイズ０．２〜０．７μｍを有するポリプロピレン不
織布よりなるフィルターを貼着した本発明にかかる培養
用容器を用いてパン酵母（Ｓ．ｃｅｒｅｖｉｓｉａｅ）
を振盪培養した。即ち、市販のペースト状パン酵母より
分離したパン酵母（Ｓ．ｃｅｒｅｖｉｓｉａｅ）を使用
し、Ｙ−Ｍ寒天斜面培地上で、３０℃、２４時間培養し
た後に、２白金の菌体を酵母増殖用培地２００ｍｌに接
種し、本発明の培養容器を用いて３０℃、１８時間、２
２０ｒｐｍの回転で培養した。酵母増殖用培地には、
０．２％ＭｇＳＯ4・７Ｈ2Ｏ、０．４％（ＮＨ4）2ＳＯ
4、０．８％ＫＨ2ＳＯ4、０．８％ｙｅａｓｔ ｅｘｔ
ｒａｃｔ、３％ｇｌｕｃｏｓｅからなる液体培養池（Ｐ
Ｈ ６）を用いた。本培養は酵母増殖用培地に対して５
％（Ｖ／Ｖ）の前培養液を加えた後、培養温度３０℃で
行なった。培養の結果を図３に示した。なお、比較例と
して５００ｍｌ容の三角フラスコを使用して同様に行な
った。その結果を図３に示した。図３における分析方法
は次の方法で測定した。Ｕ．Ｏ．Ｄ．（ｕｎｉｔ ｏｐ
ｔｉｃａｌ ｄｅｎｎｓｉｔｙ）単位菌体濃度は、波長
５８０ｎｍを用いてで分光分析計（島津ボシュロム分光
分析計スペクロニック２０Ａ）で定量した。エタノール
（ｇ／ｌ）定量は、Ｃｈｒｏｍｏｓｏｒｂ １０５（６
０−８０メッシュ）を充填した内径５ｍｍ、長さ２ｍガ
ラスカラムを使用した。Ｇｌｕｃｏｓｅ（ｇ／ｌ）は、
ムスロターゼ・ＧＯＧ（和光純薬グルコース−ＣII−テ
ストワコー）を用いて定量した。Example 1 A bottom surface having a width of 130 mm and a depth of 100 mm and a height of 160
A spout member having an inner diameter of 15 mm was attached to the opening of the rectangular parallelepiped mm by thermal bonding. Then, a through hole having a width of 60 mm and a length of 30 mm is provided at two places of a front surface and a rear surface, and a filter made of a polypropylene nonwoven fabric having a pore size of 0.2 to 0.7 μm is adhered to the through hole. Baker's yeast (S. cerevisiae) using a container
Was cultured with shaking. That is, after using a baker's yeast (S. cerevisiae) isolated from a commercially available pasty baker's yeast and culturing it on a Y-M agar slant medium at 30 ° C. for 24 hours, 2 platinum cells were transformed into a yeast growth medium. Inoculate 200 ml and use the culture vessel of the present invention at 30 ° C. for 18 hours for 2 hours.
The culture was performed at a rotation of 20 rpm. Yeast growth media include:
0.2% MgSO 4 · 7H 2 O , 0.4% (NH 4) 2 SO
4 , 0.8% KH 2 SO 4 , 0.8% yeast ext
cult, a liquid culture pond composed of 3% glucose (P
H 6) was used. The main culture was performed on 5
% (V / V) of the preculture, and the culture was performed at a culture temperature of 30 ° C. The results of the culture are shown in FIG. The same procedure was performed using a 500 ml Erlenmeyer flask as a comparative example. The result is shown in FIG. The analysis method in FIG. 3 was measured by the following method. U. O. D. (Unit op
The cell density was determined using a spectrophotometer (Shimadzu Bausch & Lomb spectrometer 20A) using a wavelength of 580 nm. Ethanol (g / l) determination was performed using Chromosorb 105 (6
(0-80 mesh), a glass column having an inner diameter of 5 mm and a length of 2 m was used. Glucose (g / l)
It was quantified using Muslotase GOG (Wako Pure Chemical Industries Glucose-CII-Test Wako).
【００１１】[0011]
【発明の効果】本発明においては、以上述べたような構
成をしたので振盪中に培養液がほぼ円流となり、培養効
率は良く、しかも注出口部材を取り付けてあるので、容
器の取扱も簡便で植菌時の取扱性も良く、さらにガス抜
き用弁を使用した場合は容器の内部から外部へのみガス
が流出して、外部の雑菌の侵入が無く培養容器として優
れた効果を奏する。According to the present invention, the above-described structure allows the culture solution to flow substantially circularly during shaking, has a high culture efficiency, and has a spout member so that handling of the container is easy. In addition, when the inoculation valve is used, the gas flows out only from the inside of the container to the outside, so that there is no invasion of external germs and an excellent effect is obtained as a culture container.
【図１】本発明にかかる培養用容器の斜視図FIG. 1 is a perspective view of a culture vessel according to the present invention.
【図２】折り畳んだ状態の本発明にかかる培養用容器の
平面図FIG. 2 is a plan view of a culture vessel according to the present invention in a folded state.
【図３】実施例（Ａ）及び比較例（Ｂ）の培養結果を示
した図FIG. 3 is a diagram showing the culture results of Example (A) and Comparative Example (B).
【図４】先に提案された容器の斜視図FIG. 4 is a perspective view of a previously proposed container.
１ 容器 ２ 包装材 ３ 表面材 ４
裏面材 ５ 側面面材 ６ 側縁融着部 ７ 底辺融着部 ８
上部融着部 ９ 注出口部材 １０ 折込み線 １１ 貫通孔 １
２ 封口治具DESCRIPTION OF SYMBOLS 1 Container 2 Packaging material 3 Surface material 4
Back surface material 5 Side surface material 6 Side edge fusion part 7 Bottom fusion part 8
Upper fusion part 9 Spout member 10 Folding line 11 Through hole 1
2 Sealing jig
フロントページの続き (72)発明者 須藤 禎子 東京都中央区日本橋馬喰町１丁目４番16号 藤森工業株式会社内Continuation of front page (72) Inventor Sadako Sudo 1-4-16-1 Nibashi Bakurocho, Chuo-ku, Tokyo Inside Fujimori Industry Co., Ltd.
Claims (2)
材で構成され、開口部に注出口部材を突設したガゼット
袋よりなる振盪培養用容器であって、該ガゼット袋の開
口部の長さが、ガゼット袋の襞部の長さと前記注出口部
材の基部の長さの和に等しく、且つ、該ガゼット袋の上
部に少なくとも１か所に貫通孔を穿設して、該部分にフ
ィルター材料を貼着したことを特徴とする振盪培養用容
器。1. A container for shaking culture comprising a gusset bag comprising a packaging material having a heat-adhesive synthetic resin layer in an inner layer and having a spout member protruding from an opening. The length is equal to the sum of the length of the fold of the gusset bag and the length of the base of the spout member, and at least one through hole is formed in the upper part of the gusset bag, and A container for shaking culture, on which a filter material is stuck.
けた請求項１記載の振盪培養用容器。2. The container for shaking culture according to claim 1, wherein a valve is attached to a spout member projecting from the opening.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP30835896A JPH10150972A (en) | 1996-11-19 | 1996-11-19 | Container for shaking culture |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP30835896A JPH10150972A (en) | 1996-11-19 | 1996-11-19 | Container for shaking culture |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH10150972A true JPH10150972A (en) | 1998-06-09 |
Family
ID=17980111
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP30835896A Pending JPH10150972A (en) | 1996-11-19 | 1996-11-19 | Container for shaking culture |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH10150972A (en) |
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| JP2002330750A (en) * | 2001-05-11 | 2002-11-19 | Hideo Tanaka | Flask for shaking culture and device for shaking culture |
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1996
- 1996-11-19 JP JP30835896A patent/JPH10150972A/en active Pending
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|---|---|---|---|---|
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| JP2005295904A (en) * | 2004-04-13 | 2005-10-27 | Toyo Seikan Kaisha Ltd | Culture container and culture method |
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