JPH10113097A - Insect breeding device and insect breeding method - Google Patents
Insect breeding device and insect breeding methodInfo
- Publication number
- JPH10113097A JPH10113097A JP9228519A JP22851997A JPH10113097A JP H10113097 A JPH10113097 A JP H10113097A JP 9228519 A JP9228519 A JP 9228519A JP 22851997 A JP22851997 A JP 22851997A JP H10113097 A JPH10113097 A JP H10113097A
- Authority
- JP
- Japan
- Prior art keywords
- breeding
- ozone
- breeding apparatus
- insect
- insects
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Abstract
Description
【０００１】[0001]
【発明の属する技術分野】本発明は昆虫の飼育装置の殺
菌方法に関する。The present invention relates to a method for sterilizing an insect breeding apparatus.
【０００２】[0002]
【従来の技術】近年、遺伝子組換えウイルスを昆虫に感
染させ、昆虫の体内で有用物質を生産する研究が行なわ
れており、例えば蚕にウイルスを感染させネコのウイル
ス病治療に使うインターフェロンの生産が知られてい
る。（特開平３−１３９２７６号公報）また、蚕の無菌
飼育方法について、特開平８−５１８９１号公報には、
活性空気を供給する方法が開示されている。2. Description of the Related Art In recent years, studies have been conducted to infect insects with a genetically modified virus and produce useful substances in the body of the insect. For example, production of interferon used to treat virus in cats by infecting silkworms with the virus. It has been known. (Japanese Unexamined Patent Publication (Kokai) No. 3-139276) The aseptic breeding method of silkworms is disclosed in Japanese Unexamined Patent Publication (Kokai) No. 8-51891.
A method for supplying active air is disclosed.
【０００３】[0003]
【発明が解決しようとする課題】遺伝子組換えウイルス
等に感染した昆虫を飼育するには、使用前の飼育装置が
細菌やウイルス等で汚染されていないよう殺菌や不活化
を十分行なう必要がある。また飼育中に遺伝子組換えウ
イルス等が系外に発散し、環境を汚染することのないよ
うウイルス等を不活化や殺菌する必要がある。更に飼育
が終了した後の飼育装置内に付着したウイルス等につい
ても系外発散を防止するため不活化や殺菌を行う必要が
ある。In order to breed insects infected with a genetically modified virus or the like, it is necessary to sufficiently sterilize or inactivate the breeding apparatus before use so that the breeding apparatus is not contaminated with bacteria or viruses. . In addition, it is necessary to inactivate and sterilize the virus or the like so that the genetically modified virus or the like will emanate out of the system during breeding and will not pollute the environment. Furthermore, it is necessary to inactivate and sterilize viruses and the like adhering to the breeding device after breeding is completed in order to prevent the virus from spreading outside the system.
【０００４】本発明はこのような事情に鑑みてなされた
ものであり、使用前の飼育装置の細菌汚染が原因で昆虫
が感染するのを防止したり、系外の環境に対しては飼育
中あるいは飼育終了後において系内のウイルス等で汚染
させないことを目的とするものである。The present invention has been made in view of such circumstances, and prevents insects from being infected due to bacterial contamination of a breeding apparatus before use, or breeds the breeding apparatus in an environment outside the system. Alternatively, the purpose of the present invention is to prevent the virus from being contaminated after the rearing.
【０００５】[0005]
【課題を解決するための手段】本発明はこのような目的
を達成するための次のような構成をとる。SUMMARY OF THE INVENTION The present invention has the following structure to achieve the above object.
【０００６】すなわち、本発明の昆虫飼育装置はオゾン
発生装置を昆虫飼育装置の吸気部、排気部または装置内
に装着したもの、あるいはオゾン発生装置を昆虫飼育装
置の外に設置し、ダクト等で昆虫飼育装置の吸気部また
は排気部と連結したもの、またはオゾンを含有する気体
を供給できる装置を同様な部署に持った昆虫飼育装置か
らなる。In other words, the insect breeding apparatus of the present invention has an ozone generator mounted in the intake section, the exhaust section or the inside of the insect breeding apparatus, or the ozone generator is installed outside the insect breeding apparatus, and is provided with a duct or the like. An insect breeding apparatus connected to the intake or exhaust section of the insect breeding apparatus, or having an apparatus capable of supplying gas containing ozone in a similar department.
【０００７】このように本発明に係る昆虫の飼育装置に
おいては、昆虫を飼育する前にオゾン発生装置を通して
オゾンを含有した空気を飼育装置内に供給することによ
り、使用する飼育装置内に存在する細菌やウイルス等を
オゾンの持つ強力な殺菌力により死滅や不活化させるこ
とができ、これらの細菌やウイルス等から飼育する昆虫
への感染を未然に防止できる。[0007] As described above, in the insect breeding apparatus according to the present invention, the air containing ozone is supplied into the breeding apparatus through the ozone generator before breeding the insect, so that the insect breeding apparatus is present in the used breeding apparatus. Bacteria and viruses can be killed or inactivated by the strong bactericidal power of ozone, and infection of breeding insects from these bacteria and viruses can be prevented.
【０００８】またオゾン発生装置を昆虫飼育装置内部に
装着し、飼育装置内の空気を循環することにより、使用
する飼育装置内に存在する細菌やウイルス等をオゾンの
持つ強力な殺菌力により同様に死滅や不活化させること
ができる。Further, by mounting the ozone generator inside the insect breeding apparatus and circulating the air inside the breeding apparatus, bacteria and viruses existing in the breeding apparatus to be used are similarly disinfected by the strong sterilizing power of ozone. Can be killed or inactivated.
【０００９】飼育装置内の温度・湿度は、昆虫を飼育す
る通常の条件が採用できるが、本発明の効果を十分に達
成するためには、湿度はＲＨ５０％以上が好ましく、さ
らに好ましくはＲＨ６０％〜ＲＨ９０％である。The temperature and humidity in the breeding apparatus can be the same as those for breeding insects. However, in order to sufficiently achieve the effects of the present invention, the humidity is preferably RH 50% or more, and more preferably RH 60%. RH 90%.
【００１０】なおオゾンの供給は、紫外線ランプ式やコ
ロナ放電式、電解法等のオゾン発生装置を介するものの
みでなく、オゾンを含有する気体を供給できる装置でも
よい。The supply of ozone is not limited to an ozone generator such as an ultraviolet lamp type, a corona discharge type, and an electrolytic method, but may be a device capable of supplying a gas containing ozone.
【００１１】紫外線ランプを用いる場合は１００〜２０
０ｎｍの波長の紫外線ランプが好ましく用いられる。When an ultraviolet lamp is used, 100 to 20
An ultraviolet lamp having a wavelength of 0 nm is preferably used.
【００１２】オゾン発生装置から発生させるオゾンの量
は、殺菌すべき菌類、菌の量、殺菌すべき気体の量や飼
育装置の大きさなどを考慮して選択すれば良い。The amount of ozone generated by the ozone generator may be selected in consideration of the fungi to be sterilized, the amount of bacteria, the amount of gas to be sterilized, the size of the breeding apparatus, and the like.
【００１３】更に飼育装置から排出される空気をオゾン
発生装置に通すことにより、例えば遺伝子組換えウイル
ス等を感染させた昆虫の飼育において、系外に排出され
る空気に混入している遺伝子組換えウイルス等を死滅や
不活化させることができ、ウイルスが系外に発散して環
境を汚染することを防止できる。Further, by passing air discharged from the breeding apparatus through an ozone generator, for example, in rearing of insects infected with a genetically modified virus, etc., the genetically modified Viruses and the like can be killed or inactivated, and the virus can be prevented from escaping out of the system and polluting the environment.
【００１４】[0014]
【発明の実施の形態】以下に、本発明の望ましい実施形
態を図面を参照して説明する。Preferred embodiments of the present invention will be described below with reference to the accompanying drawings.
【００１５】図１は本発明の一実施態様に係る昆虫の飼
育装置を示している。図において１は昆虫飼育装置本体
を示している。FIG. 1 shows an insect breeding apparatus according to one embodiment of the present invention. In the figure, reference numeral 1 denotes an insect breeding apparatus main body.
【００１６】飼育装置本体１には温度・湿度調節機２が
ダクト３を介して接続されている。ダクト３にはオゾン
発生装置４が取り付けてある。温度・湿度調節機２の吸
気口５は装置全体の吸気口となっており、高性能のフィ
ルター６が取り付けてある。装置に供給する系外の空気
中の細菌やウイルスなどはフィルターで除去できるよう
になっている。吸気口５から入った空気はダクト３を通
る間に、オゾン発生装置４から発生するオゾンを含んで
飼育装置に供給され、飼育装置内に存在している細菌や
ウイルスなどを死滅や不活化させる。昆虫の飼育前に飼
育装置内を殺菌した後はオゾン発生装置４の電源を切
り、オゾンの発生を止めて飼育中の昆虫への影響を防止
する。A temperature / humidity controller 2 is connected to the breeding apparatus main body 1 via a duct 3. An ozone generator 4 is attached to the duct 3. The air inlet 5 of the temperature / humidity controller 2 is an air inlet of the entire apparatus, and a high-performance filter 6 is attached. Bacteria and viruses in the air outside the system to be supplied to the device can be removed with a filter. The air that has entered through the air inlet 5 is supplied to the breeding apparatus while containing the ozone generated from the ozone generator 4 while passing through the duct 3, and kills or inactivates bacteria and viruses existing in the breeding apparatus. . After the inside of the breeding apparatus is sterilized before the breeding of the insects, the power of the ozone generator 4 is turned off, and the generation of ozone is stopped to prevent the effects on the breeding insects.
【００１７】飼育装置本体１の上面には排気口７が設け
られており、さらに排気ダクト８が接続している。排気
ダクト８には吸気口側と同様のオゾン発生装置９が取り
付けてある。したがって排気口７側においては、系内か
らの細菌やウイルス、例えば昆虫に感染させた細菌やウ
イルスの系外への排出が阻止できるようになっている。
このオゾン発生装置９は昆虫の飼育中も使用する。An exhaust port 7 is provided on the upper surface of the breeding apparatus main body 1, and an exhaust duct 8 is further connected thereto. An ozone generator 9 similar to that on the intake port side is attached to the exhaust duct 8. Therefore, on the exhaust port 7 side, discharge of bacteria and viruses from the system, for example, bacteria and viruses infected to insects, from the system can be prevented.
The ozone generator 9 is used during the breeding of insects.
【００１８】[0018]
参考例１ ＜細胞変性効果によるウイルス濃度の定量＞文献１の方
法に従って、組換えウイルス濃度を定量した。すなわ
ち、組換えカイコ核多角体病ウイルスを感染させたカイ
コ幼虫体液抽出液、または飼育装置の排気を通したカイ
コ培養細胞培養上清を希釈し、５×１０5個／ｍｌのＢ
Ｍ−Ｎ細胞培養液に添加した。２７℃で１０日間培養し
た後に、顕微鏡観察によってＢＭ−Ｎ細胞に対する細胞
変性効果を確認し、感染性ウイルス量を算定した。感染
性ウイルス量は、文献２に従ってＴＣＩＤ５０（５０％
tissue culture infectious dose）を求めることによ
って決定した。Reference Example 1 <Quantification of virus concentration by cytopathic effect> The recombinant virus concentration was quantified according to the method of Reference 1. That is, a silkworm larva body fluid extract infected with a recombinant silkworm nuclear polyhedrosis virus or a silkworm culture cell culture supernatant passed through the exhaust of a breeding apparatus is diluted, and 5 × 10 5 cells / ml of B
Added to MN cell culture. After culturing at 27 ° C for 10 days, the cytopathic effect on BM-N cells was confirmed by microscopic observation, and the amount of infectious virus was calculated. The amount of infectious virus was determined by TCID50 (50%
tissue culture infectious dose).
【００１９】実施例１ ２１００（幅）×１１００（奥行）×１６００（高さ）
ｍｍの飼育装置本体に飼育条件をコントロールする温度
・湿度調節機を付け、２５℃、ＲＨ７０％に調節した。
温度・湿度調節機から飼育装置本体へ接続する３２０
（縦）×４００（横）×１１００（長さ）ｍｍの空気ダ
クトに、オゾン発生装置としてオゾンが時間当り１１５
０ｍｇ生成する１８４．９ｎｍの紫外線を発する出力４
５Ｗの水銀ランプを２基取り付けた。飼育装置本体に入
る空気は温度・湿度調節機に付けた高性能のフィルター
を通し、系外からの細菌やウイルスなどをフィルターで
除去できるようになっている。また飼育装置排気部にも
ダクトを設置し、同様の水銀ランプを取り付けた。ダク
トの出口は室外に出した。Example 1 2100 (width) × 1100 (depth) × 1600 (height)
A temperature / humidity controller for controlling breeding conditions was attached to the breeding apparatus main body of mm, and the temperature and humidity were adjusted to 25 ° C. and 70% RH.
320 connecting the temperature and humidity controller to the breeding device body
Ozone is used as an ozone generator in an air duct of (vertical) × 400 (horizontal) × 1100 (length) mm.
Output 4 that emits 184.9 nm ultraviolet light that produces 0 mg
Two 5 W mercury lamps were attached. The air entering the breeding equipment body passes through a high-performance filter attached to a temperature and humidity controller so that bacteria and viruses from outside the system can be removed by the filter. A duct was also installed in the exhaust of the breeding equipment, and a similar mercury lamp was installed. The exit of the duct went out of the room.
【００２０】飼育２日前からオゾン発生装置を作動さ
せ、２ｍ3／分の空気を供給してオゾン濃度約９〜５ppm
で飼育装置を殺菌した。Two days before breeding, the ozone generator was activated, and air of 2 m 3 / min was supplied to provide an ozone concentration of about 9 to 5 ppm.
Sterilized the breeding equipment.
【００２１】オゾン発生装置の電源を切り、飼育装置の
トレーに人工飼料と特開平３−１３９２７６に示された
方法によって作製した遺伝子組換えウイルスを接種した
５令の蚕を５０００頭入れ、２ｍ3／分の空気を供給し
て５日間飼育した。The ozone generator was turned off, and 5000 breeds of 5th-instar silkworms inoculated with artificial feed and the genetically modified virus prepared by the method disclosed in JP-A-3-139276 were placed in the tray of the breeding apparatus, and 2 m 3 Breeding for 5 days with air supply per minute.
【００２２】また蚕飼育中は飼育装置排気部のオゾン発
生装置を作動させ、系内からの蚕に感染させた遺伝子組
換えウイルスの系外への排出を阻止した。During the rearing of the silkworms, the ozone generator at the exhaust of the rearing device was operated to prevent the transgenic virus infected with the silkworms from being discharged from the system.
【００２３】飼育装置内の蚕は、尾脚を切って採取した
体液を２００〜４００倍の顕微鏡で観察したが、雑菌等
による感染は見られず、参考例１の方法でウイルスの増
殖を調べた結果、接種した遺伝子組換えウイルスに感染
した。The silkworm in the breeding apparatus was observed under a microscope of 200 to 400 times the body fluid collected by cutting the tail legs, but no infection by various bacteria was observed, and the virus growth was examined by the method of Reference Example 1. As a result, the cells were infected with the inoculated genetically modified virus.
【００２４】また系外への排気を、カイコ培養細胞の培
養液に通しその上清を参考例１の方法で培養したが、遺
伝子組換えウイルスは不活化しており、検出されなかっ
た。The exhaust gas was passed through the culture of silkworm cultured cells, and the supernatant was cultured by the method of Reference Example 1. The recombinant virus was inactivated and was not detected.
【００２５】[0025]
【発明の効果】本発明によれば、昆虫を飼育する前にオ
ゾンを含有した空気を飼育装置内に供給することによ
り、使用する飼育装置内に存在する細菌やウイルス等を
オゾンにより死滅や不活化させることができ、これらの
細菌やウイルス等から飼育する昆虫への感染を未然に阻
止し、昆虫を望ましい状態で飼育できる。また細菌やウ
イルスなどが系外に発散し、環境を汚染することを防ぐ
ことができる。According to the present invention, by supplying ozone-containing air to the breeding apparatus before breeding the insects, bacteria and viruses existing in the breeding apparatus to be used can be killed or immortalized by the ozone. It can be activated, preventing infection of the breeding insects from these bacteria and viruses, etc., and breeding the insects in a desirable state. In addition, it is possible to prevent bacteria and viruses from escaping out of the system and polluting the environment.
【００２６】参考文献 １．Ｊ．Ｋ．Ｙａｍａｍｏｔｏら：Ｖｅｔ．Ｉｍｍｕｎ
ｏｌ．ａｎｄ Ｉｍｍｕｎｏｐａｔｈｏｌ．，１１，１
−１９（１９８６） ２．モダンバイオロジーシリーズ２３、動物組織培養法
（１９７６）Ｐ２９６−３００、共立出版References 1. J. K. Yamamoto et al .: Vet. Immun
ol. and Immunopathol. , 11,1
-19 (1986) 2. Modern Biology Series 23, Animal Tissue Culture Method (1976) P296-300, Kyoritsu Publishing
【図１】本発明に係る昆虫飼育装置の概略構成図であ
る。FIG. 1 is a schematic configuration diagram of an insect breeding apparatus according to the present invention.
１ 飼育装置本体 ２ 温度・湿度調節機 ３ ダクト ４ オゾン発生装置 ５ 吸気口 ６ 高性能フィルター ７ 排気口 ８ 排気ダクト ９ オゾン発生装置 DESCRIPTION OF SYMBOLS 1 Breeding apparatus main body 2 Temperature / humidity controller 3 Duct 4 Ozone generator 5 Intake port 6 High-performance filter 7 Exhaust port 8 Exhaust duct 9 Ozone generator
Claims (8)
気部または装置内に備えることを特徴とする請求項１記
載の昆虫飼育装置。2. The insect breeding apparatus according to claim 1, wherein the ozone generating device is provided in an intake part, an exhaust part, or inside the breeding apparatus.
し、オゾンを飼育装置内に送りこむことを特徴とする請
求項１記載の昆虫飼育装置。3. The insect breeding apparatus according to claim 1, wherein the ozone generator is installed outside the breeding apparatus, and the ozone is sent into the breeding apparatus.
の波長の紫外線ランプを有することを特徴とする請求項
１〜３のいずれか１項記載の昆虫飼育装置。4. An ozone generator, which has a thickness of 100 to 200 nm.
The insect breeding apparatus according to any one of claims 1 to 3, further comprising an ultraviolet lamp having a wavelength of:
育装置を用いて昆虫を飼育することを特徴とする昆虫飼
育方法。5. An insect breeding method, comprising breeding insects using the breeding apparatus according to claim 1.
接触させることを特徴とする請求項５記載の昆虫飼育方
法。6. The insect breeding method according to claim 5, wherein the breeding apparatus before breeding the insects is brought into contact with ozone.
ることを特徴とする請求項５記載の昆虫飼育方法。7. The insect breeding method according to claim 5, wherein ozone is brought into contact with the exhaust gas during breeding the insects.
５〜７のいずれか１項記載の昆虫飼育方法。8. The method for breeding insects according to claim 5, wherein the insects are silkworms.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP9228519A JPH10113097A (en) | 1996-08-23 | 1997-08-25 | Insect breeding device and insect breeding method |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP8-222720 | 1996-08-23 | ||
| JP22272096 | 1996-08-23 | ||
| JP9228519A JPH10113097A (en) | 1996-08-23 | 1997-08-25 | Insect breeding device and insect breeding method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH10113097A true JPH10113097A (en) | 1998-05-06 |
Family
ID=26525030
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP9228519A Pending JPH10113097A (en) | 1996-08-23 | 1997-08-25 | Insect breeding device and insect breeding method |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH10113097A (en) |
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| CN110999876A (en) * | 2019-11-27 | 2020-04-14 | 江西修水养蚕人实业有限公司 | Silkworm breeding disinfection equipment of dusting based on monitoring data |
| JP2021000070A (en) * | 2019-06-20 | 2021-01-07 | 广州分格食品科技有限公司 | Animal/plant cell aseptic culture tray |
| JP2021168928A (en) * | 2015-11-05 | 2021-10-28 | ウシオ電機株式会社 | Sterilization method |
| CN115299412A (en) * | 2022-08-15 | 2022-11-08 | 那坡同益新丝绸科技实业有限公司 | Silkworm rearing room with ozone sterilization function |
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1997
- 1997-08-25 JP JP9228519A patent/JPH10113097A/en active Pending
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2021168928A (en) * | 2015-11-05 | 2021-10-28 | ウシオ電機株式会社 | Sterilization method |
| CN109042556A (en) * | 2018-10-25 | 2018-12-21 | 罗城仫佬族自治县群富种养农民专业合作社 | Silkworm net |
| JP2021000070A (en) * | 2019-06-20 | 2021-01-07 | 广州分格食品科技有限公司 | Animal/plant cell aseptic culture tray |
| CN110999876A (en) * | 2019-11-27 | 2020-04-14 | 江西修水养蚕人实业有限公司 | Silkworm breeding disinfection equipment of dusting based on monitoring data |
| CN115299412A (en) * | 2022-08-15 | 2022-11-08 | 那坡同益新丝绸科技实业有限公司 | Silkworm rearing room with ozone sterilization function |
| CN115299412B (en) * | 2022-08-15 | 2023-07-18 | 那坡同益新丝绸科技实业有限公司 | Silkworm room with ozone sterilization function |
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