JPH10108691A - Detection of antimicrobial activity - Google Patents

Abstract

PROBLEM TO BE SOLVED: To assess and detect the antimicrobial activity of the vapor generated from a test sample through such process that the test sample and a culture means containing microorganisms provided for the test are collocated in a specified volume of the space inside a vessel body provided with an opening followed by culturing the microorganisms isolatedly from the outside air. SOLUTION: A microorganism culturing means 3 inoculated with microorganisms provided for the test and a test sample 2 are collocated in the space 5-200L in volume inside a vessel body 1 provided with an opening 4 through which a sample container and the means 3 are passable, a water vapor feed means 5 is also collocated in the space so as to prevent the space from getting dry, and the space is virtually isolated from the outside air and the system as a whole is left at rest for a specified time. Based on the growing state of the microorganisms, the antimicrobial activity of the vapor generated from the test sample 2 is assessed; furthermore, after the assessment, the means 3 is taken out, and observed after the culture to assess the bacteriostatic and/or germicidal activity of the test sample 2, thus detecting the objective antimicrobial activity.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

TECHNICAL FIELD The present invention relates to a novel method for detecting antimicrobial activity and an antibacterial fragrance selected by the method. More specifically, the present invention relates to a method for detecting the growth of a fragrance or an essential oil such as an essential oil. It relates to a method for detecting antimicrobial activity, such as inhibitory activity, bacteriostatic activity and / or bactericidal activity.

[0002]

2. Description of the Related Art In recent years, as the interest in hygiene has generally increased, the demand for products having antimicrobial activity such as growth inhibitory activity, bacteriostatic activity and / or bactericidal activity against microorganisms such as bacteria or mold has rapidly increased. Have been doing. Among materials having antimicrobial activity as materials constituting these products, in particular, fragrances, essential oils, etc. are recognized as having a wide use value in view of their transpiration, aroma, safety, convenience, economy, etc. , Is getting much attention.

[0003] Hitherto, various detection methods for finding and identifying a sample having such antimicrobial activity have been proposed, for example, the following methods.

First, a method for detecting antimicrobial activity in a liquid state: (1) Method for measuring minimum inhibitory concentration (MIC): A test microorganism is inoculated into a medium in which test samples of various concentrations are dissolved, and (2) Minimum bactericidal concentration (MBC) measurement method: A test sample-containing medium in which growth of microorganisms was not performed in (1) above was added to a fresh medium for further observation. Then, the bacteriostatic (microbial growth) and sterilization (non-microbial growth) concentrations are measured, and (3) filter paper disc method (halo test method, see FIG. 2): Petri dish (h = 15 mm, d = 90) After inoculating the test microorganism on the solidified agar medium in mm), a filter paper impregnated with the test sample is placed and cultured to observe the growth inhibition zone (halo zone).

[0005] The above method (3) is extremely simple and can rapidly determine the activity. However, the size of the zone of inhibition is determined not only by the antimicrobial activity of the sample used but also by the diffusivity of the sample in the medium. Because it can vary depending on the permeability, it is not always a method that can provide a reliable criterion.

When it is desired to detect the antimicrobial activity of the vapor generated from the test sample, the antimicrobial activity of the liquid is not always reflected in the activity in a vaporized state. Detection methods intended to determine antimicrobial activity in a vapor state have been proposed. That is, (4) filter paper disk method (see FIG. 3): inoculating the test microorganism on an agar medium solidified in a Petri dish (h)
= 15 mm, d = 90 mm), a filter paper impregnated with the test sample is placed on the lower side and cultured, and the growth inhibition zone is observed. (5) Aluminum cap method: impregnated with the test sample of (4) Place 0.5 mL of the sample in an aluminum cap (diameter 20 mm, height 5 mm) instead of the filter paper, and observe the formation of a growth inhibition zone in the same manner. (6) Glass bottle method (see FIG. 4): 33 m inner diameter
A 10-mL agar medium inoculated with the test microorganism was placed in the bottom of a cylindrical glass bottle with a height of 67 mm and a height of 67 mm. Sa50
The culture is suspended at a position of about mm, and the activity is determined based on the depth of the inhibition zone. (7) Modified aluminum cap method (see FIG. 5): The same aluminum cap as in (5) above was placed at the center of the petri dish. Microorganisms were inoculated by solidifying an agar medium around it, and 1 mL of the test sample was placed in an aluminum cap.
Incubate at 37 ° C. Then, depending on the degree of growth of the microorganism, the aluminum cap is sealed to stop the evaporation of the sample into the petri dish space, and further left still for 72 hours to judge the bacteriostasis and sterilization. (8) Plastic chamber method (See FIG. 6): In order to measure the concentration of the test sample in the space, a 10 × 10 × 10 cm cubic plastic chamber was solidified with an agar medium inoculated with the test microorganism, and a predetermined amount of After juxtaposing and culturing the containers containing the test samples, the activity is determined based on the growth state of the microorganism, and the sample remaining in the container is weighed to determine the weight of the sample evaporated and volatilized in the space. There is a method of calculating the amount of volatilization as a value obtained by dividing the volume by the volume of the space.

In these conventional methods, the sample is used in a space of about 0.035 to 0.085 L and a volume of about 0.24 mL to 29 mL when converted to a space having a volume of 20 to 1000 μL, that is, 1 L. , The concentration of the sample vapor becomes high, resulting in a supersaturated state immediately after the start of the incubation. Therefore, there is a concern that the test sample may be adsorbed, dissolved, and diffused into the medium, and there is a high possibility that dew condensation will occur during the incubation. Therefore, in these methods, the effects of the sample liquid coming into contact with the microorganism, and ( 3)
In the above, the action derived from the diffusivity and the permeability described above is also observed, and it cannot be said that the desired activity is detected based on the action of the vapor generated from the test sample.
In the methods (4) to (6), particularly, since the distance between the test microorganism growth medium and the test sample is short, the vapor of the sample keeps coming into contact with the medium at an extremely high concentration. It is considered large.

[0008]

A simple and accurate detection method for measuring the antimicrobial activity of the vapor generated from a test sample has not been proposed so far.
The present invention provides a method for detecting such antimicrobial activity,
An object of the present invention is to provide an antibacterial fragrance which is shown to generate a vapor having antimicrobial activity by the detection method.

[0009]

Means for Solving the Problems The present invention has been accomplished as a result of intensive studies to achieve the above-mentioned object in the antimicrobial detection method, and the gist thereof is as follows. It is.

(1) A method for detecting an antimicrobial activity, comprising: a container having a test sample container and a container body having an opening through which a test microorganism culturing means can be inserted and removed; A step of juxtaposing the microorganism culturing means and the test sample inoculated with the test microorganism, a step of substantially blocking the space from the outside air and leaving the space to stand for a predetermined time, and a step of generating from the test sample from the growth state of the microorganism. A detection method comprising the step of determining the antimicrobial activity of the vapor.

(2) A kit for detecting an antimicrobial activity of a test sample, comprising a test sample container and an opening through which a test microorganism culturing means can be taken in and out, and having a volume of 5 to 2
Steam generated from a test sample, including a container body enclosing a space of 00 L, a container for a test sample capable of evaporating a test sample into the space of the container body, a test microorganism culturing means, and / or a steam supply means. A kit for detecting an antimicrobial activity having the kit.

(3) According to the method for detecting antimicrobial activity,
Samples that have been shown to generate vapors with antimicrobial activity.

(4) According to the method for detecting antimicrobial activity,
An antimicrobial fragrance that has been shown to generate antibacterial vapors.

(5) A composition containing one or more antibacterial fragrances.

That is, the inventors of the present invention perform detection using a small amount of a test sample with respect to the volume of a space in a relatively large space, thereby detecting the activity of the sample liquid component, the diffusivity in the medium, The present inventors have found that the antimicrobial activity of the vapor itself generated from the sample can be determined irrespective of its properties, and the present invention has been completed. By measuring according to such a method, it is clarified that even the fragrance having no activity recognized by the conventional method has antibacterial properties, and therefore, the antibacterial fragrance and a composition containing the same are newly provided by the present invention. Is what is done.

[0016]

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, embodiments of the present invention will be described in detail.

The detection method of the present invention comprises, for example, (1) a container for a test sample 2 and an opening 4 through which a test microorganism culturing means 3 can be inserted and removed, as in an embodiment of the present invention shown in FIG. A microorganism culturing means 3 inoculated with a test sample and a test microorganism is juxtaposed in a space having a volume of 5 to 200 L in which the container body 1 is included. (2) The space is substantially shut off from outside air for a predetermined time. Standing, and (3) from the state of growth of the microorganism,
A step of determining an antimicrobial activity of the vapor generated from the test sample.

Further, after the determination, it is preferable to include a step of taking out the microorganism culturing means 3 and observing it after culturing to determine the bacteriostatic and / or bactericidal activity of the test sample. Means for supplying steam
It is good to include the step of adding 5.

The test sample 2 may be in the form of a liquid or a solid. Various types of samples whose vapor generated from the sample is desired to determine the antimicrobial activity can be used as the test sample of the present invention. It will not be done. For example, a liquid selected from the group consisting of fragrances, essential oils, and mixtures thereof is preferable as the test sample. Test sample 2
For example, in the case of a stock solution of a liquid sample such as a fragrance or an essential oil, 1 to 50 μL, preferably 5
Used in a volume of 2020 μL. It is considered that by providing a fragrance, an essential oil and the like in this amount, the space does not become supersaturated by the vapor of the sample, and the activity of the liquefied sample can be prevented from being measured.

It is preferable that the sample is placed in a container capable of diffusing gas generated from the test sample 2 into the space of the container body 1. Such a container is, for example, a petri dish, a watch glass, a beaker, or a crucible, and is made of a suitable size made of a material that is not deteriorated by a sample, such as a glass having a diameter of 3 to 10 cm and a height of 0.5 to 2 cm. A dish may be used.

When the test sample 2 is a liquid, the transpiration rate can be controlled by regulating the transpiration area by impregnating the sample with a support such as filter paper or nonwoven fabric and placing the sample in a container. The size of the support is, for example, 50% when a liquid fragrance is used as a test sample.
It is about × 50 × 0.5 mm.

As the test microorganism to be inoculated into the medium of the microorganism culturing means 3, various microorganisms which are targets of antimicrobial activity, such as molds, bacteria, etc., can be applied. As the mold, black mold ( Aspergillus niger ), blue mold ( Pen
icillium citrinum , black mold ( Cladosporium clados)
poriodes ) and bacteria, for example, staphylococci, especially Staphylococcus aureus ,
Pseudomonas aeruginosa or Escherichia coli ( Esch
erichia coli ).

These microorganisms are inoculated, for example, into a microorganism culturing means 3 such as a petri dish which contains a medium in which a test microorganism can grow and whose inoculated surface can contact the space. As this medium, from the viewpoint of usability, price, etc., preferably potato extract, agar medium containing glucose, meat extract,
A medium containing peptone, sodium chloride, glucose, yeast extract and the like can be mentioned. Such a medium, for example, a commercially available petri dish having a diameter of 3 to 8 cm and a height of 10 to 15 mm,
It may be solidified so as to have a thickness of 5 to 10 mm, and the test microorganism may be inoculated by a conventional method. As shown in FIG. 1 (b), it is also possible to put a plurality of microorganism culturing means 3 in the container body 1 at the same time to perform the test. In this case, the microorganism culture means
The types of microorganisms seeded in 3 may be the same or different from each other. The microorganism culturing means may be placed at an appropriate place in the container body 1, or may be attached to the inner surface or the inside of the lid.

The water vapor supply means 5 is provided in order to prevent the microorganism culture means 3 from drying out in the space of the container main body 1 and not hindering the growth of microorganisms. In a 5 L container (Fig. 1 (a)), a 50 x 50 x 5 mm filter paper impregnated with 5 to 10 mL of sterilized water is placed in a container such as a petri dish, or in a 200 L container ( FIG.
When conducting the test in (b)), add 100 to a 500 mL beaker.
A solution containing 200 mL of sterilized water is used. Each element described above is stored, and the container body 1 for measuring the antimicrobial activity is preferably made of a material that is preferably transparent and the inside of which can be observed.
Aluminum or stainless steel can be used, but it is preferable to make the inside aseptic, so materials that can be subjected to commonly used sterilization operations are suitable, and furthermore, operability, impact resistance, and chemical resistance Glass is preferred in terms of properties and the like. The container body 1 preferably contains a space having a volume of 5 to 200 L, preferably 5 L. By performing the measurement in a space of this size, the activity of the vapor itself generated from the test sample can be measured. It becomes possible. Furthermore, in the case of such a 5 L space, the inner bottom area is 150 to 2,800 cm ² and the height is 20 to 100.
cm, preferably 30 to inside of the bottom area 300 to 350 cm ² and a height
It should have 35 cm. The container body 1 is provided with an opening 4 having a diameter of, for example, about 10 to 15 cm, through which the container for the test sample 2 and the test microorganism culturing means 3 can be taken in and out. With such a shape, the test sample and the test microorganism can be arranged in a positional relationship suitable for measuring the antimicrobial activity in the vapor state, that is, the vapor from the test sample 2 Without facing the culturing means 3, the appropriate concentration of the vapor reaches the microorganism and its antimicrobial activity can be accurately detected. The opening 4 is substantially shielded from the outside air and the space by a lid member or the like when the antimicrobial activity is measured.
In addition, the container body 1 has an
Preferably, a grip 6 is formed.

In the step (b), the standing for measuring the antimicrobial activity is carried out at 20 to 40 ° C., preferably at 27 to 37 ° C.
The reaction is carried out for 17 to 200 hours, preferably for 72 to 168 hours. This step is performed in an incubator or the like as necessary. Needless to say, the above conditions depend on the characteristics of the test microorganism, especially when the test microorganism is a mold such as Aspergillus niger, blue mold or blackening fungus.
For days, or in the case of bacteria such as staphylococcus, Pseudomonas aeruginosa or Escherichia coli, the cells are cultured at about 37 ° C. for 24 hours.

When determining the antimicrobial activity of the vapor generated from the test sample in the step (c), first,
(A) If the growth of microorganisms is observed after step (b),
The test sample is determined not to have antimicrobial activity. If no growth of microorganisms is observed,
Cover 3 and take it out, observe it after further culturing at the same temperature as in step (b) for 24-200 hours, (b) if the microorganism starts to grow, bacteriostatic, and (c) the growth of the microorganism If it is not recognized, it is determined that it has bactericidal activity. These determination results are excellent indicators of the antimicrobial activity of the vapor generated from the test sample. This is because, as described above, the preferred concentration of the vapor reaches the test microorganism culturing means and acts on the microorganisms present on the surface. Therefore, it is possible to detect an antimicrobial activity that could not be detected by the conventional method, and at the same time, to correctly determine a false positive activity that is considered to have only detected the activity in a liquid state in the conventional method. It becomes possible.

The present invention also relates to a kit for detecting the antimicrobial activity, which essentially comprises the container body, a container for a test sample, and a microorganism culturing means and / or a water vapor supplying means. A sample support as described above can also be used for the test sample container.

Further, according to the present invention, there is provided a sample which generates a vapor having an antimicrobial activity, the antimicrobial activity of which was not recognized by the conventional detection method. These samples are, for example, antibacterial fragrances that have been shown to generate antibacterial vapors by the antimicrobial activity detection method described above. In particular, antibacterial fragrances that generate vapor having antibacterial properties against Staphylococcus aureus include hexyl acetate, hexenyl acetate, hexylcinnamic aldehyde, geranyl nitrile or zestvel (Zestover, manufactured by Firmenich), and antibacterial properties against Pseudomonas aeruginosa As an antibacterial fragrance that generates a vapor having an antibacterial property against Escherichia coli, decenal is used.

The present invention further provides compositions containing one or more of these antimicrobial vapor-generating antimicrobial fragrances. Such compositions are provided in various forms such as liquids, solids, and gels by appropriately adding additives such as excipients, preservatives, and stabilizers in accordance with the conventional method for producing aromatic compositions, providing means, and the like. Can be done.

[0030]

EXAMPLES The present invention will be further described below with reference to examples, but the present invention is not limited to these disclosures.

The antimicrobial activity of the vapor generated from various fragrances was examined by the antimicrobial activity detection method of the present invention, and compared with the results obtained by the conventional filter paper disk method.

(Examples) The antibacterial properties of each of the flavors described in Tables 1 and 2 against three kinds of test bacteria, namely, Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli were examined as follows.

An internal bottom area of 255 is shown schematically in FIG.
The following elements were put into a glass container main body 1 having a holding portion 6 and an opening 4 having a diameter of 10 cm at the top, having a cm ² , a height of 32 cm, and an internal space volume of 5 L, and having an opening 4 having a diameter of 10 cm at the top. The container for test sample 2 was a glass Petri dish with a diameter of 8 cm and a height of 1 cm.
A sample support of 50 × 50 × 0.5 mm was placed, and 50, 100 or 200 μL of each sample was added. As the microorganism culturing means 3, 20 mL of a normal agar medium was injected into a plastic petri dish having a diameter of 8.5 cm and a height of 1.2 cm, and solidified, and each of the bacteria was inoculated with a platinum loop. As a water vapor supply means, a glass petri dish having a diameter of 8 cm and a height of 1 cm, in which 10 mL of sterilized water was impregnated with filter paper of 50 × 50 × 5 mm was used.

[0034] Put these three elements in a container body,
The cells were cultured in an incubator for 24 hours, and the growth of the bacteria was observed. If the growth of the bacteria was observed, the cells were judged as "no activity (x)". Next, the microorganism culturing means in which the growth of bacteria was not recognized was covered, taken out from the container body, and further cultured at 37 ° C. for 24 hours. Those in which the growth of bacteria was observed after the completion of the culture were those having "bacteriostatic activity (O)" and those in which no growth of bacteria was observed,
It was determined to have “bactericidal activity (◎)”. The results are shown in Tables 1 and 2. Here, there was no difference in the results of each experiment depending on the amount of the sample of 50, 100 or 200 μL.

(Comparative Example) Antibacterial properties of the same test sample and test bacteria used in the examples were examined by a filter paper disk method (see FIG. 3). The same agar medium as used in the microorganism culturing means of the example was solidified in a plastic petri dish having a diameter of 8.5 cm and a height of 1.2 cm, and each microorganism was inoculated in the same manner as in the example. Put the Petri dish lid on and flip it over, 50 × 50 in the center of the lid part
Place a × 0.5 mm filter paper, and test samples 10, 20 or 30μ
L was added. After culturing at 37 ° C. for 24 hours, the diameter (mm) of the inhibition zone was measured and used as an indicator of antibacterial activity. If no inhibition zone was observed, it was judged as "no activity (x)". Tables 1 and 2 show the results when 20 μL of the sample was used. In the table,
If the growth of the fungus is suppressed on the entire dish,
∞, and those having different judgment results depending on the amount of the sample used are indicated by *. Such a difference in the determination results reflects that a strong effect is obtained basically depending on the increase in the amount of use. For example, regarding the effect of benzaldehyde on Pseudomonas aeruginosa, a halo band was formed when the test sample volume was 10 μL, and
Although microbial colony formation was slightly observed in μL, the growth of bacteria was almost completely suppressed on the entire dish (レ *).
At 0 μL, it was suppressed on the whole petri dish.

[0036]

[Table 1]

[0037]

[Table 2]

From Tables 1 and 2, differences in the results of antibacterial detection by each detection method became apparent. In view of the basic structure of the fragrance, neither the detection method of the present invention nor the filter disk method, many fragrances having a terpene or linear structure exhibit antibacterial properties against these bacteria, and in view of the functional groups of the fragrance. In the detection method of the present invention, many of those having an alcohol, an aldehyde or an ester have an activity.
It became clear that many of those having alcohol or aldehyde exhibited antibacterial activity against these bacteria. In addition, other fragrances in which a difference in antibacterial properties was recognized by both detection methods are nitrogen compounds, phenol, and the like.

Also, in view of the results of the bacterial types, many fragrances are found to be active against Staphylococcus aureus by the method of the present invention, and active against Pseudomonas aeruginosa and Escherichia coli by the filter paper disk method. Many were recognized.

Table 3 summarizes the results.

[0041]

[Table 3]

As shown in Table 3, although the antibacterial property was recognized by the conventional filter paper disk method, the activity was not recognized by the method of the present invention for detecting the antibacterial property of the vapor from the test sample. (A), on the other hand, a fragrance (B) whose activity was not detected by the conventional method but whose activity was recognized by the detection method of the present invention was revealed. Also,
A fragrance in which the inhibition zone is detected by a conventional method and a bacteriostatic effect is suggested. In the detection method of the present invention, a fragrance (C) having a bactericidal activity is recognized and a fragrance having a bactericidal effect suggested by a conventional method. In the detection method of the present invention, a fragrance (D) showing bacteriostatic activity was also confirmed.

[0043]

As described above, according to the method for detecting an antimicrobial activity of the present invention, an appropriate concentration of vapor reaches a test microorganism culturing means from a test sample. And / or antimicrobial activity, such as growth inhibitory activity, bacteriostatic activity and / or bactericidal activity, of the vapor generated from the test sample, irrespective of properties such as diffusivity and permeability.

Accordingly, an appropriate index for selection of a fragrance, an essential oil, and the like which can be suitably used as a material of a composition having an antimicrobial activity of transpiration is provided.

In addition, when the conventional method for measuring antimicrobial activity was used, the antibacterial activity of the vapor generated from the fragrance which had no activity was found, and the use of these fragrances in antibacterial compositions and the like was found. The present invention newly suggests the possibility of the present invention, and makes it possible to provide such a composition.

[Brief description of the drawings]

FIG. 1 shows an embodiment of the method for detecting antimicrobial activity of the present invention.
It is a schematic diagram showing (a) and (b).

FIG. 2 is a schematic cross-sectional view showing an embodiment of a conventional filter paper disc method.

FIG. 3 is a schematic sectional view showing an embodiment of a conventional filter paper disk method.

FIG. 4 is a schematic sectional view showing an embodiment of a conventional glass bottle method.

FIG. 5 is a schematic cross-sectional view showing a modification of the conventional aluminum cap method.

FIG. 6 is a schematic sectional view showing an embodiment of a conventional plastic chamber method.

[Description of sign]

 1 Container body 2 Test sample 3 Test microorganism culturing means 4 Opening 5 Moisture evaporation means 6 Grip

──────────────────────────────────────────────────の Continued on the front page (51) Int.Cl. ⁶ Identification symbol FI A61K 7/46 A61K 7/46 A A61L 9/01 A61L 9/01 PC12M 1/34 C12M 1/34 B (72) Inventor Günther Holzner Swiss C-H-1227 Geneva 8 Rute des Genes 1 Firmenich Societe Anonym

Claims (16)

[Claims] 1. A method for detecting an antimicrobial activity, comprising the following steps: a container for a test sample and a container body having an opening through which a test microorganism culturing means can be inserted and removed;
In the space, the microorganism culturing means inoculated with the test microorganism and the test sample are juxtaposed, and the space is substantially shut off from the outside air and allowed to stand for a predetermined period of time. A method for determining the antimicrobial activity of the steam. 2. The detection method according to claim 1, further comprising, after the determination, culturing and observing the microorganism by a microorganism culturing means to determine the bacteriostatic and / or bactericidal activity of the test sample. 3. The detection method according to claim 1, further comprising a step of providing a steam supply means for preventing drying in the space. 4. The test microorganism culturing means is a petri dish containing a medium in which the test microorganism can grow and a microorganism inoculated in the medium and whose inoculated surface can contact the space. 3. The detection method according to any one of 3. 5. The detection method according to claim 1, wherein the test sample is selected from the group consisting of a fragrance, an essential oil, and a mixture thereof. 6. The test sample is placed in a space 1 of the container body.
The detection method according to claim 5, wherein the detection method is used in an amount of 1 to 50 µL based on L. 7. The method according to claim 1, wherein the test microorganism is a mold.
7. The detection method according to any one of claims 1 to 6. 8. The method according to claim 1, wherein the test microorganism is a bacterium.
7. The detection method according to any one of claims 1 to 6. 9. The method according to claim 8, wherein the bacterium is selected from the group consisting of staphylococci, Pseudomonas aeruginosa and Escherichia coli. 10. A kit for detecting an antimicrobial activity of a test sample, comprising a test sample container and an opening through which a test microorganism culturing means can be taken in and out, and having a volume of 5 to 200.
A container body enclosing the space L, a container for a test sample capable of evaporating the test sample into the space of the container body, a test microorganism culturing means, and / or a steam supply means; Kit for detecting antimicrobial activity. 11. A sample which has been shown to generate vapor having antimicrobial activity by the method for detecting antimicrobial activity according to claim 1. 12. An antibacterial fragrance which has been shown to generate antibacterial vapor by the method for detecting antimicrobial activity according to claim 9. 13. The antimicrobial fragrance according to claim 12, wherein the antimicrobial fragrance is hexyl acetate, hexenyl acetate, hexylcinnamic aldehyde, or geranyl nitrile. 14. The antibacterial fragrance according to claim 12, wherein the antibacterial fragrance is desenal. 15. The antibacterial fragrance is benzaldehyde,
The antibacterial fragrance according to claim 11, which is hexylcinnamic aldehyde or benzyl acetate. 16. A composition comprising one or more antibacterial fragrances according to claim 12.