JPH0940573A - Inhibitor of amylase and composition for inhibiting amylase - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain a safe inhibitor of an amylase, containing an extract of Moutan Cortex as an active ingredient without any problem in safety for human bodies due to its use of the Moutan Cortex which is a crude drug that has hither been used as a Chinese medicine or a folk medicine and capable of effec tively inhibiting activities of the amylase. SOLUTION: This inhibitor of an amylase contains an extract of Moutan Cortex as an active ingredient. For example, a root bark of Paeonia suffruticosa Andrews can be used as the Moutan Cortex. The extraction is preferably carried out by using hydrous ethanol at 80 deg.C for 1-5hr. The extracting operations are preferably performed 2-5 times. When the objective inhibitor is used as a medicinal composition, the daily dose thereof for an adult is preferably 0.5-2,000mg, expressed in terms of the active ingredient at a time and administered three times before a meal. When the inhibitor is blended in a food, the amount thereof blended is preferably 0.01-30wt.% based on the total amount of the food.

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to an amylase inhibitor extracted from peony skin, and an amylase inhibitor composition containing the amylase inhibitor and used as a medicine or a food.

[0002]

2. Description of the Related Art In recent years,
Obesity resulting from dietary habits such as satiation, overeating, and gourmet has become an important issue not only for young women but also for a wide range of age groups due to its close relationship with adult diseases such as diabetes. As a means for eliminating this obesity, methods such as exercising to burn fat and restricting diet can be considered. However, exercise is difficult to continue,
It is difficult to eliminate obesity by these methods because not only is dietary restriction accompanied by mental distress, but the burden of calorie calculation is large. In addition, it is said that adult diseases such as diabetes and gallstones which are closely related to obesity are often triggered by an increase in blood sugar due to excessive intake of starch and the like.

From these things, recently, as a simple prevention and treatment method for obesity, a method of suppressing the digestion of carbohydrates such as starch, which accounts for 60% of the energy source of the Japanese, has been considered. In other words, when eating starch foods such as rice and udon, which are the center of Japanese dietary habits, a method of suppressing the digestion of starch by taking an amylase inhibitor that inhibits the activity of amylase, which is a digestive enzyme of starch, etc. Is being considered.

Against this background, amylase inhibitors have recently attracted attention as an effective means for preventing and treating obesity, and various types are obtained mainly from seeds of seeds such as legumes and microorganisms. Oligosaccharide (JP-A-63-483)
It is known that a substance having an amylase inhibitory action is contained in JP-A No. 02).

However, these substances have problems in terms of effectiveness of amylase inhibitory action, etc., and most of them have not yet reached the stage of practical use.

The present invention has been made in view of the above circumstances, and provides a novel amylase inhibitor and a composition for inhibiting amylase which have no problems in terms of safety and effectiveness as pharmaceuticals and foods. With the goal.

[0007]

[Means for Solving the Problems and Embodiments of the Invention] As a result of intensive investigations by the present inventors in order to achieve the above-mentioned object, the extract from peony skin, which has been used as a herbal medicine for many years and a folk medicine, is excellent. It has been found that it has an amylase inhibitory action, and has completed the present invention.

That is, the present invention relates to an amylase inhibitor characterized by comprising an extract from peony skin as an active ingredient, and an amylase inhibitor composition used as a drug or a food, wherein the amylase inhibitor is Provided is a composition for amylase inhibition, which is contained as an active ingredient.

The present invention will be described in more detail below. In the present invention, the peony skin is peony (Paeo).
nia suffruticosa Andrews)
, And the root bark of this plant can be used, but the species is not particularly limited. The peony skin extract of the present invention may be extracted from peony skin powder.

The method for obtaining the peony skin extract applied to the present invention is not particularly limited, and a usual extraction method is adopted.
Examples of the extraction solvent include alcohols such as methanol, ethanol and butanol, aliphatic hydrocarbons such as hexane, heptane and cyclohexane, esters such as ethyl acetate, organic solvents such as ketones such as acetone and water. Can be used singly or in combination of two or more. Considering the extraction efficiency of the amylase inhibitor and the toxicity when the extraction solvent remains, etc., among the above extraction solvents, ethanol is particularly preferable. A mixture of water and water is preferred. Here, the extraction time is preferably 0.5 to 10 hours, particularly preferably 1 to 5 hours. The extraction temperature may be room temperature, but it is preferable to perform the extraction by heating to 80 ° C. The extraction operation may be performed once, but is preferably performed 2 to 5 times.

When the extraction solvent is a non-toxic one such as water, ethanol or hydrous ethanol, the amylase inhibitor of the present invention may be used as it is or the extract obtained by the above extraction procedure. It can also be used as a liquid. Further, it may be a concentrated extract, and may be prepared as a dry powder or a paste by concentrating the dried solid or the extract and then freeze-drying. Then, if necessary, a purification step may be added to enhance the amylase inhibitory activity of the extract.

The amylase inhibitor of the present invention can be used as a drug by incorporating other drugs used in drugs, quasi drugs, etc. and a pharmaceutically acceptable base or food.
It can be an amylase-inhibiting composition used as a health food or food.

When the amylase-inhibiting composition of the present invention is used as a medicine, for example, an excipient, a coloring agent, a lubricant,
Binders, disintegrants, coating agents, stabilizers, preservatives and the like can be added. In this case, the mixing ratio of each component and the amylase inhibitor is not particularly limited, and a ratio suitable for formulation thereof can be appropriately selected. Further, examples of the dosage form of the formulation of the composition (medicine) containing these include tablets, capsules, powders, fine granules, granules and the like.

Although the dose of the above-mentioned drug varies depending on the condition of the disease and is not constant, it is usually preferable to take 0.5 to 2000 mg as an active ingredient per adult 3 times a day before meals.

When the amylase-inhibiting composition of the present invention is used as a food, it may be of any type, for example, starch-containing foods such as bread, noodles, biscuits, cakes, etc., as well as rice. Ingestible furikake, side foods such as sausage, ham and kamaboko, desserts such as jelly and pudding, dairy products such as yogurt, ice cream and milk, confectionery such as gummy candies, candy, chocolate and gum, drinks such as juice and drinks. Is mentioned. In this case, it is preferable that the amylase inhibitor is mixed in an amount of 0.001 to 100% by weight, particularly 0.01 to 30% by weight, based on the total amount of the food.

[0016]

The amylase inhibitor of the present invention is a herbal medicine,
Since an extract of peony skin, which is a kind of crude drug used as a folk medicine, is used as an active ingredient, there is no problem in safety for human body and amylase activity can be effectively inhibited. Therefore, the amylase-inhibiting composition containing this amylase inhibitor is suitably used as a medicine and a food.

[0017]

EXAMPLES The present invention will be specifically described below with reference to examples and comparative examples, but the present invention is not limited to the following examples.

[Example 1] 100 g of peony powder (sold by Uchida Wakan Yaku Co., Ltd.) in 50% by volume of an aqueous ethanol solution 5
It was immersed in 00 ml and extracted under boiling for 1 hour. This operation was repeated twice to collect the extract. The obtained extract was concentrated under reduced pressure at 60 ° C with an evaporator and freeze-dried to obtain 15 g of an extract. When the extract was dissolved in methanol to 10 mg / ml and filtered, the activity inhibition rate of α-amylase (derived from Bacillus subtilis, sold by: Wako Pure Chemical Industries, Ltd.) was measured by the following measuring method. 41.6% of the activity was inhibited. Method for measuring α-amylase inhibitory activity 1) 0.5 wt% soluble starch, 20 mM CaCl ₂ , 1
50 mM acetate buffer (pH 6.
To 5), an appropriate amount of α-amylase enzyme solution (derived from Bacillus subtilis) and 20 μl of the sample are added to make the total amount 0.5 ml. (In ice bath) 2) Incubate at 37 ° C for 15 minutes to carry out reaction,
Immediately stop the reaction in an ice bath. 3) Iodine reagent (1 / 6000M iodine, 1 / 600M
Add 5 ml of potassium iodide and 1/600 M hydrochloric acid, and add 7
The absorbance at 00 nm is measured. As controls, those without enzyme (Control 1: OD ₇₀₀ = about 1.2) and those with further enzyme (Control 2: OD ₇₀₀ = about 0.2) are measured at the same time. 4) The suppression rate is calculated from the following formula. Inhibition rate (%) = (Sample-Control 2) / (Control 1-Control 2)
× 100

Next, animal (SD rat, male, 4 weeks old)
Each group consisted of 5 animals, 5% of the above extract was added to the feed, and the group was bred for 3 weeks, and the control group was the group bred in the same manner with the feed containing no extract.
Then, the feed efficiency obtained from the weight gain and the feed intake of the animals of each group after being raised for 3 weeks was calculated by the following formula, and the fat percentage was calculated from the body weight of the animals of each group and the epididymal adipose tissue weight. . The results are shown in Table 1. Feed efficiency (%) = weight gain (g) ÷ feed intake (g)
× 100

[0020]

[Table 1]

[Comparative Example 1] The activity inhibition rate of the peony skin powder was measured by the method described in Example 1, and the effect was compared with the extract obtained in Example 1. As a result, it was confirmed that the activity inhibition rate was 6.3%, which was much lower than that of the extract obtained in Example 1.

[Example 2] Lactose (940 g), corn starch (340 g), crystalline cellulose (200 g), magnesium stearate (10 g) and the extract obtained in Example 1 (10 g) were directly compressed into tablets to give tablets (for amylase inhibition). Composition).

Example 3 350 mg of the extract obtained in Example 1 was dispersed and dissolved in 250 ml of hot water, and 6.0 g of coffee powder and 5.0 g of erythritol were added,
Finally, 5.0 g of gelatin was added and then refrigerated to obtain coffee jelly (composition for amylase inhibition).

Front Page Continuation (72) Inventor Sonoko Miyamoto 1-3-7 Honjo, Sumida-ku, Tokyo Lion Co., Ltd. (72) Hideaki Ozeki 1-3-7 Honjo, Sumida-ku, Tokyo Lion Shares In the company

Claims (2)

[Claims] 1. An amylase inhibitor comprising an extract from peony skin as an active ingredient. 2. A composition for amylase inhibition, which is used as a medicine or food, and which contains the amylase inhibitor according to claim 1 as an active ingredient.