JPH09325144A - Aerobic biodegrability measuring apparatus - Google Patents
Aerobic biodegrability measuring apparatusInfo
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- JPH09325144A JPH09325144A JP14262296A JP14262296A JPH09325144A JP H09325144 A JPH09325144 A JP H09325144A JP 14262296 A JP14262296 A JP 14262296A JP 14262296 A JP14262296 A JP 14262296A JP H09325144 A JPH09325144 A JP H09325144A
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- carbon dioxide
- microorganisms
- trapping
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- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
【０００１】[0001]
【発明の属する技術分野】本発明は、微生物による有機
物の分解の程度を測定し、これによって、環境の汚染の
程度や廃棄物処理の処理状況、また、微生物の生分解の
活性度を測定する好気的生分解度測定装置に関する。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention measures the degree of decomposition of organic substances by microorganisms, thereby measuring the degree of environmental pollution, the state of waste treatment, and the activity of biodegradation of microorganisms. The present invention relates to an aerobic biodegradability measuring device.
【０００２】[0002]
【従来の技術】従来、環境の汚染の程度や廃棄物処理の
処理状況、また、微生物の生分解の活性度等の好気的生
分解度の測定は、微生物が培養液中で活動することによ
り消費した酸素量を生物化学的酸素要求量（ＢＯＤ値）
として求め、このＢＯＤ値を指標として行っている。2. Description of the Related Art Conventionally, the measurement of aerobic biodegradation such as the degree of environmental pollution, the treatment status of waste treatment, and the biodegradation activity of microorganisms is based on the fact that the microorganisms are active in the culture solution. The amount of oxygen consumed by the biochemical oxygen demand (BOD value)
And the BOD value is used as an index.
【０００３】ＢＯＤ計は、培養槽中において好気的微生
物の呼吸によって消費した酸素の減少を検出し、消費さ
れた酸素を水の電気分解等の方法によって補給して培養
液の酸素分圧を一定に保ち、培養中に補給された酸素量
をポテンショメータ等を含むクーロメトリーなどの方法
によって求める装置であり、図６は従来から知られてい
るＢＯＤ計の概略構成図を示している。The BOD meter detects a decrease in oxygen consumed by respiration of aerobic microorganisms in the culture tank, supplements the consumed oxygen by a method such as electrolysis of water, and determines the oxygen partial pressure of the culture solution. FIG. 6 shows a schematic configuration diagram of a conventionally known BOD meter, which is a device that keeps constant and determines the amount of oxygen replenished during culture by a method such as coulometry including a potentiometer.
【０００４】図６において、ＢＯＤ計は恒温槽１０７内
に培養槽１０１とマノメータ１０７を備え、微生物によ
って消費した酸素量を測定部１１０によって測定する。
培養槽１０１内の試料１００中に存在する微生物は、培
養槽１０１の気相中または試料中の酸素を使って呼吸を
行い試料１００中の有機物質を水と二酸化炭素に分解す
る。放出された二酸化炭素を二酸化炭素吸収剤１０３が
吸収すると、培養槽１０１中の圧力は低下する。マノメ
ータ１０７は培養槽１０１内の圧力低下を検出して、リ
レー回路１１１を介してスイッチ１１２を閉じて定電流
源１１３によって電解セル１０６を駆動し、酸素を発生
して補給を行う。発生した酸素によって培養槽１０１内
の圧力が高まると、電解セル１０６による酸素発生は停
止する。培養中に補給した酸素量は、定電流源１１３か
らの電流量により求めることができる。In FIG. 6, the BOD meter is provided with a culture tank 101 and a manometer 107 in a constant temperature tank 107, and the measuring unit 110 measures the amount of oxygen consumed by microorganisms.
Microorganisms present in the sample 100 in the culture tank 101 breathe using oxygen in the gas phase of the culture tank 101 or in the sample to decompose organic substances in the sample 100 into water and carbon dioxide. When the carbon dioxide absorbent 103 absorbs the released carbon dioxide, the pressure in the culture tank 101 decreases. The manometer 107 detects the pressure drop in the culture tank 101, closes the switch 112 via the relay circuit 111, drives the electrolysis cell 106 by the constant current source 113, and generates oxygen to replenish it. When the pressure in the culture tank 101 is increased by the generated oxygen, the oxygen generation by the electrolysis cell 106 is stopped. The amount of oxygen supplemented during the culture can be obtained from the amount of current from the constant current source 113.
【０００５】[0005]
【発明が解決しようとする課題】従来の微生物が培養液
中で活動することにより消費した酸素量から求めた生物
化学的酸素要求量（ＢＯＤ値）は、かならずしも微生物
による有機物の分解の程度を表す好気的生分解度の良好
な指標とならず、ＢＯＤ計は必ずしも正確な好気的生分
解度測定装置ではないという問題点がある。The biochemical oxygen demand (BOD value) obtained from the amount of oxygen consumed by the activity of conventional microorganisms in a culture medium always represents the degree of decomposition of organic substances by microorganisms. There is a problem that the BOD meter is not always an accurate aerobic biodegradability measuring device because it does not serve as a good indicator of the aerobic biodegradability.
【０００６】従来のＢＯＤ計では、培養液中の微生物の
活動によって発生するガスは全て二酸化炭素であるとし
て吸収し、吸収したガスに対応した酸素を補給し、この
酸素量によって測定を行っている。しかしながら、培養
液中において微生物は有機物を分解して二酸化炭素を発
生する以外に、二酸化硫黄，硫化水素，蟻酸や酢酸等の
揮発性酸類などの有機物の分解に係わらないガス成分を
発生しているため、ＢＯＤ値は、かならずしも微生物に
よる有機物の分解の程度を表していない。In the conventional BOD meter, all the gas generated by the activity of microorganisms in the culture solution is absorbed as carbon dioxide, oxygen corresponding to the absorbed gas is supplied, and the amount of oxygen is used for measurement. . However, in the culture solution, in addition to decomposing organic matter to generate carbon dioxide, microorganisms generate gas components such as sulfur dioxide, hydrogen sulfide, and volatile acids such as formic acid and acetic acid that are not related to the decomposition of organic matter. Therefore, the BOD value does not always represent the degree of decomposition of organic substances by microorganisms.
【０００７】また、従来のＢＯＤ計は二酸化炭素吸収剤
として顆粒状のカセイソーダからなるソーダライムを使
用しているが、該ソーダライムは培養槽中の水分を吸収
して、二酸化炭素の吸収能力が低下するという問題点も
ある。Further, the conventional BOD meter uses soda lime consisting of granular caustic soda as a carbon dioxide absorbent. The soda lime absorbs water in the culture tank and has an ability to absorb carbon dioxide. There is also the problem of lowering.
【０００８】そこで、本発明は従来の問題点を解決し、
培養液中の微生物による有機物の分解の程度を表す好気
的生分解度を正確に求めることができる好気的生分解度
測定装置を提供することを目的とする。Therefore, the present invention solves the conventional problems,
An object of the present invention is to provide an aerobic biodegradation degree measuring device capable of accurately obtaining the degree of aerobic biodegradation, which indicates the degree of decomposition of organic matter by microorganisms in a culture solution.
【０００９】[0009]
【課題を解決するための手段】本発明の好気的生分解度
測定装置は、微生物による有機物の分解により発生する
二酸化炭素を捕集液内に捕集する二酸化炭素捕集部と、
捕集液から二酸化炭素を分離する分離部と、分離した二
酸化炭素を検出する二酸化炭素検出部と、少なくとも二
酸化炭素捕集部から分離部へ捕集液を送液する送液部と
を備え、検出した二酸化炭素量に基づいて生分解度を測
定することによって、微生物による有機物の分解の程度
を表す好気的生分解度を正確に求める。An aerobic biodegradation degree measuring device of the present invention comprises a carbon dioxide trapping portion for trapping carbon dioxide generated by decomposition of organic matter by microorganisms in a trapping liquid,
A separation unit that separates carbon dioxide from the collected liquid, a carbon dioxide detection unit that detects the separated carbon dioxide, and a liquid feed unit that feeds the collected liquid from at least the carbon dioxide collection unit to the separation unit, By measuring the degree of biodegradation based on the detected amount of carbon dioxide, the degree of aerobic biodegradation, which represents the degree of decomposition of organic substances by microorganisms, is accurately obtained.
【００１０】本発明の好気的生分解度測定装置は、微生
物が有機物を分解して発生する二酸化炭素を捕集し、該
二酸化炭素量を求めることによって生分解度を測定する
ものであり、これによって、有機物以外の分解により発
生するガス成分の影響を除去して正確な微生物による有
機物の分解の程度を求めることができる。また、従来の
ＢＯＤ計が使用する二酸化炭素吸収剤は二酸化炭素の除
去を目的とするのに対して、本発明の好気的生分解度測
定装置における捕集液は、発生した二酸化炭素量の測定
を目的とし、分離部において捕集液から二酸化炭素を分
離することによって二酸化炭素の濃縮を行って、微小量
の測定を可能としている。また、本発明の好気的生分解
度測定装置は、送液部によって捕集液を送液することに
より、二酸化炭素の測定を自動で行うことができる。The aerobic biodegradability measuring device of the present invention is for measuring the biodegradability by collecting carbon dioxide generated by the decomposition of organic matter by microorganisms and determining the amount of carbon dioxide. As a result, it is possible to remove the influence of gas components generated by decomposition other than the organic matter and accurately determine the degree of decomposition of the organic matter by the microorganism. Further, while the carbon dioxide absorbent used in the conventional BOD meter is intended to remove carbon dioxide, the collected liquid in the aerobic biodegradation measuring apparatus of the present invention is the amount of generated carbon dioxide. For the purpose of measurement, carbon dioxide is concentrated in the separation unit by separating it from the collected liquid, and a minute amount can be measured. Further, the aerobic biodegradability measuring device of the present invention can automatically measure carbon dioxide by feeding the collected liquid by the liquid feeding unit.
【００１１】本発明の好気的生分解度測定装置におい
て、培養液中の微生物は有機物を分解して二酸化炭素を
発生する。密閉された二酸化炭素捕集部内のアルカリ捕
集液は、発生した二酸化炭素を良好に吸収する。送液部
は、二酸化炭素捕集部内の捕集液を取り出して分離部に
送液する。分離部は、送液された捕集液から二酸化炭素
を分離し、二酸化炭素検出部は、分離した二酸化炭素を
検出して二酸化炭素量を測定する。これによって、微生
物が有機物を分解して発生した二酸化炭素を、直接測定
することができる。In the aerobic biodegradation degree measuring apparatus of the present invention, the microorganisms in the culture solution decompose organic matters to generate carbon dioxide. The alkali scavenging liquid in the sealed carbon dioxide scavenging section satisfactorily absorbs the generated carbon dioxide. The liquid sending part takes out the collected liquid in the carbon dioxide collecting part and sends it to the separating part. The separation unit separates carbon dioxide from the collected collected liquid, and the carbon dioxide detection unit detects the separated carbon dioxide and measures the amount of carbon dioxide. As a result, carbon dioxide generated by the decomposition of organic matter by the microorganism can be directly measured.
【００１２】本発明の第１の実施態様において、二酸化
炭素捕集部内の捕集液は、送液部によって外部の捕集液
タンクから補給するものであり、ペレスターポンプによ
って、同量の捕集液を二酸化炭素捕集部内へ補給し分離
部から送液することができる。本発明の第２の実施態様
において、分離部は中空糸膜を備え、これによって、捕
集液中から二酸化炭素の気体を分離する。In the first embodiment of the present invention, the collected liquid in the carbon dioxide collecting unit is supplied from the external collecting liquid tank by the liquid feeding unit, and the same amount of the collected liquid is collected by the Pelestar pump. The collected liquid can be replenished into the carbon dioxide collecting unit and sent from the separating unit. In the second embodiment of the present invention, the separation section includes a hollow fiber membrane, which separates carbon dioxide gas from the collected liquid.
【００１３】本発明の第３の実施態様は、複数個の二酸
化炭素捕集部を備え、分離部および二酸化炭素検出部に
対して捕集液を切り換えて送液するものであり、これに
よって、複数個の試料の測定を行うことができる。The third embodiment of the present invention is provided with a plurality of carbon dioxide traps and switches the trapping liquid to the separator and the carbon dioxide detector to send the liquid. It is possible to measure a plurality of samples.
【００１４】また、本発明の第４の実施態様において、
微生物が有機物を分解して発生する二酸化炭素の発生量
を測定する構成とともに、微生物が培養液中で活動する
ことにより消費した酸素量により求める生物化学的酸素
要求量（ＢＯＤ値）を測定する構成を備え、これによっ
て、本発明により求める好気的生分解度と従来から知ら
れる生物化学的酸素要求量（ＢＯＤ値）との比較を行う
ことができる。Further, in the fourth embodiment of the present invention,
A structure for measuring the amount of carbon dioxide generated by the microorganisms by decomposing organic matter, and a structure for measuring the biochemical oxygen demand (BOD value) determined by the amount of oxygen consumed by the microorganisms acting in the culture solution This makes it possible to compare the degree of aerobic biodegradation required by the present invention with the conventionally known biochemical oxygen demand (BOD value).
【００１５】[0015]
【発明の実施の形態】以下、本発明の実施の形態を図を
参照しながら詳細に説明する。図１は本発明の装置の一
構成例を説明するための図である。図１において、好気
的生分解度測定装置は、微生物によって有機物を分解し
て発生した二酸化炭素を捕集液２３内に捕集する二酸化
炭素捕集部１と、捕集液２３から二酸化炭素を分離する
分離部５と、分離した二酸化炭素を検出するガスクロマ
トグラフ６等の二酸化炭素検出部と、少なくとも二酸化
炭素捕集部１から分離部５へ捕集液を送液するペレスタ
ーポンプ４等の送液部とを備えている。Embodiments of the present invention will be described below in detail with reference to the drawings. FIG. 1 is a diagram for explaining an example of the configuration of the device of the present invention. In FIG. 1, the aerobic biodegradation measuring device comprises a carbon dioxide trap 1 for trapping carbon dioxide generated by decomposing organic matter by microorganisms in a trap 23, and a carbon dioxide from the trap 23. A separation unit 5 for separating the separated carbon dioxide, a carbon dioxide detection unit such as a gas chromatograph 6 for detecting the separated carbon dioxide, a Pellester pump 4 for sending the collected liquid from at least the carbon dioxide collection unit 1 to the separation unit 5, etc. And a liquid feeding section of.
【００１６】二酸化炭素捕集部１は、培養槽２１内に微
生物と有機物を含む培養液２４を収め、攪拌子２５によ
り攪拌が行われる。なお、攪拌子２５は、外部の図示し
ないスターラーによって駆動される。微生物は有機物を
分解して二酸化炭素を発生し、培養槽２１内の気相部分
に放出する。培養槽２１内に設けた捕集容器２２内には
捕集液２３を貯められ、捕集液２３の表面は培養槽２１
の気相側に露出している。捕集液２３は、例えば水酸化
ナトリウム溶液や水酸化バリウム溶液等のアルカリ液で
あり、気相部分に放出された二酸化炭素はこの捕集液２
３内に吸収され、良好に溶解する。The carbon dioxide trap 1 contains a culture solution 24 containing a microorganism and an organic substance in a culture tank 21 and is stirred by a stirrer 25. The stirrer 25 is driven by an external stirrer (not shown). The microorganism decomposes organic matter to generate carbon dioxide, which is released to the gas phase portion in the culture tank 21. The collection liquid 23 is stored in the collection container 22 provided in the culture tank 21, and the surface of the collection liquid 23 is the culture tank 21.
Is exposed on the gas phase side of. The trapping liquid 23 is, for example, an alkaline liquid such as a sodium hydroxide solution or a barium hydroxide solution, and the carbon dioxide released to the gas phase portion is the trapping liquid 2
It is absorbed in 3 and dissolves well.
【００１７】捕集液２３は、培養槽２１の外部に設けた
捕集液タンク３からペレスターポンプ４を通して補給さ
れ、また、捕集容器２２からペレスターポンプ４を通し
て分離部５に取り出され送液される。図１中の矢印は、
捕集液の移動方向を示している。分離部５は、中心部分
に高分子膜等で形成される中空糸膜５２が通された分離
容器５１により形成される。中空糸膜５２には捕集容器
２２から送液された捕集液２３は通され、分離容器５１
側には６方バルブ等からなるガスサンプラー５３を通し
てキャリヤーガスが供給されるとともに、ガスクロマト
グラフ６と接続されている。捕集液２３中に溶解してい
る二酸化炭素は、分離部５においてキャリヤーガス側に
分離して移動する。これによって、キャリヤーガス内に
二酸化炭素を選択的に取り出すことができる。なお、分
離部５は、中空糸膜５２内にキャリヤーを供給し、分離
容器側５１に捕集液２３を流す構成とすることもでき
る。捕集容器２２から分離部５への捕集液２３の送液は
ペレスターポンプ４により行うことができ、捕集液タン
ク３から捕集容器２２への送液と捕集容器２２から分離
部５への送液を同一のペレスターポンプ４によって行う
ことにより、捕集液の供給と送出を同期して同量だけ行
うことができる。The collection liquid 23 is replenished from the collection liquid tank 3 provided outside the culture tank 21 through the perester pump 4, and is also taken out from the collection container 22 through the perester pump 4 to the separating section 5 and sent. Be liquefied. The arrow in FIG.
The moving direction of the collected liquid is shown. The separation part 5 is formed by a separation container 51 having a hollow fiber membrane 52 formed of a polymer membrane or the like at its center. The collection liquid 23 sent from the collection container 22 is passed through the hollow fiber membrane 52, and the separation container 51
Carrier gas is supplied to the side through a gas sampler 53 composed of a six-way valve or the like, and is connected to the gas chromatograph 6. The carbon dioxide dissolved in the collected liquid 23 is separated and moved to the carrier gas side in the separation section 5. As a result, carbon dioxide can be selectively taken out into the carrier gas. The separation unit 5 may also be configured to supply the carrier into the hollow fiber membrane 52 and flow the collected liquid 23 to the separation container side 51. The collection liquid 23 can be sent from the collection container 22 to the separation unit 5 by the Pelestar pump 4, and the collection liquid tank 3 can be sent to the collection container 22 and the collection container 22 can be separated from the separation unit 5. By feeding the liquid to 5 by the same Pellester pump 4, the same amount of the collected liquid can be supplied and delivered synchronously.
【００１８】ガスサンプラー５３を介して分離部５にキ
ャリヤーガスを供給し、二酸化炭素の分離したキャリヤ
ーガスをガスクロマトグラフ６に送るには、ガスサンプ
ラー５３中の破線で示す通路を使用して行うことができ
る。ガスサンプラー５３において、キャリヤーガスは図
１中のｂに供給され、ｅを通って分離容器５１の導入口
（図１中の下方部分）に送られる。導入されたキャリヤ
ーガスは中空糸膜５２と接触して二酸化炭素を取込み、
分離容器５１の導出口（図中の上方部分）から図１中の
ｆに戻される。その後、二酸化炭素を含んだキャリヤー
ガスはａを通ってガスクロマトグラフ６に送られ、二酸
化炭素の測定が行われる。In order to supply the carrier gas to the separation section 5 through the gas sampler 53 and send the carrier gas from which carbon dioxide has been separated to the gas chromatograph 6, use the passage shown by the broken line in the gas sampler 53. You can In the gas sampler 53, the carrier gas is supplied to b in FIG. 1 and is sent to the inlet of the separation container 51 (lower part in FIG. 1) through e. The introduced carrier gas comes into contact with the hollow fiber membrane 52 and takes in carbon dioxide,
The separation container 51 is returned from the outlet (upper part in the drawing) to f in FIG. After that, the carrier gas containing carbon dioxide is sent to the gas chromatograph 6 through a, and carbon dioxide is measured.
【００１９】なお、ガスサンプラー５３中の実線で示す
通路を使用する場合には、バルブ５４を開いて、図中の
ｃ，ｆを介して分離部５にキャリヤーガスを通した後、
図中のｅ，ｆを介して排出する。これによって、捕集液
中から二酸化炭素を除去して捕集液の浄化を行うことが
できる。When the passage shown by the solid line in the gas sampler 53 is used, the valve 54 is opened, and after passing the carrier gas through the separating portion 5 through c and f in the figure,
It is discharged via e and f in the figure. As a result, carbon dioxide can be removed from the collected liquid to purify the collected liquid.
【００２０】図２は、図１に示す構成の好気的生分解度
測定装置において、培養槽２１を大気中に開放し、捕集
液２３によって大気中の約３５０ｐｐｍの二酸化炭素を
ガスクロマトグラフで測定した結果を、従来のＢＯＤ計
を用いたガスクロマトグラフの測定結果と比較して示し
ている。図２中において、Ｇ１は本発明の好気的生分解
度測定装置による測定結果を示し、Ｇ２は従来のＢＯＤ
計による測定結果を示している。また、各測定結果にお
いて、Ｐ１は空気によるピークを示し、Ｐ２は二酸化炭
素によるピークを示している。図２の測定結果から、本
発明の好気的生分解度測定によれば、従来の測定方法よ
り感度の良い測定結果を得ることができる。FIG. 2 shows an apparatus for measuring the degree of aerobic biodegradation having the structure shown in FIG. 1 in which the culture tank 21 is opened to the atmosphere, and the collected liquid 23 is used to collect carbon dioxide of about 350 ppm in the atmosphere by gas chromatography. The measurement results are shown in comparison with the measurement results of a gas chromatograph using a conventional BOD meter. In FIG. 2, G1 shows the measurement result by the aerobic biodegradation measuring apparatus of the present invention, and G2 shows the conventional BOD.
The measurement result by the meter is shown. Moreover, in each measurement result, P1 shows the peak by air and P2 shows the peak by carbon dioxide. From the measurement result of FIG. 2, according to the aerobic biodegradation measurement of the present invention, a measurement result with higher sensitivity than the conventional measurement method can be obtained.
【００２１】本発明の好気的生分解度測定装置は、図１
に示す二酸化炭素捕集部を複数個設けた構成とすること
によって、複数の試料の好気的生分解度測定に対応する
ことができる。図３は複数個の二酸化炭素捕集部を備え
た好気的生分解度測定装置の概略構成図である。図２に
おいて、第１二酸化炭素捕集部１１，第２二酸化炭素捕
集部１２，〜第ｎ二酸化炭素捕集部１ｎのｎ個の二酸化
炭素捕集部は、前記図１に示す二酸化炭素捕集部と同様
の構成とすることができ、それぞれ第１ペレスターポン
プ４１，第２ペレスターポンプ４２〜第ｎペレスターポ
ンプ４ｎのｎ個のペレスターポンプによって捕集液タン
ク３から捕集液の供給を受けるとともに、二酸化炭素を
吸収した捕集液を分離部５に送液し、ガスクロマトグラ
フ６により測定を行う。この構成では、ペレスターポン
プによって捕集液を送液する二酸化炭素捕集部を選択す
ることによって、ｎ個の二酸化炭素捕集部に対して分離
部５の個数を１個とすることができる。The aerobic biodegradability measuring apparatus of the present invention is shown in FIG.
By adopting a configuration in which a plurality of carbon dioxide traps shown in (2) are provided, it is possible to deal with aerobic biodegradation measurement of a plurality of samples. FIG. 3 is a schematic configuration diagram of an aerobic biodegradation degree measuring device including a plurality of carbon dioxide traps. In FIG. 2, the n carbon dioxide traps of the first carbon dioxide trap 11, the second carbon dioxide trap 12, to the nth carbon dioxide trap 1n are the carbon dioxide traps shown in FIG. The collecting unit may have the same configuration as that of the collecting unit, and the collecting liquid is collected from the collecting liquid tank 3 by n number of the Pelestar pumps, that is, the first Pelestar pump 41, the second Peleester pump 42 to the n-th Pelestar pump 4n. Is supplied, the collected liquid that has absorbed carbon dioxide is sent to the separation unit 5, and measurement is performed by the gas chromatograph 6. In this configuration, the number of the separation units 5 can be set to one for n carbon dioxide collection units by selecting the carbon dioxide collection unit that feeds the collection liquid by the perester pump. .
【００２２】次に、本発明の好気的生分解度測定装置
と、従来から知られるＢＯＤ計との組み合わせた構成に
ついて図４を用いて説明する。図中のＡは本発明の好気
的生分解度測定装置による炭素ガス測定部であり、Ｂは
従来から知られるＢＯＤ計の酸素要求量測定部であっ
て、微生物が培養液中で活動することにより消費した酸
素量により求める生物化学的酸素要求量（ＢＯＤ値）を
測定する構成である。Next, a configuration in which the aerobic biodegradability measuring device of the present invention and a conventionally known BOD meter are combined will be described with reference to FIG. In the figure, A is a carbon gas measuring part by the aerobic biodegradability measuring device of the present invention, B is an oxygen demand measuring part of a conventionally known BOD meter, and microorganisms are active in the culture solution. Thus, the biochemical oxygen demand (BOD value) determined by the amount of oxygen consumed is measured.
【００２３】炭素ガス測定部Ａは、酸素要求量測定部Ｂ
内に設けられた培養槽８１内に捕集液タンク３から捕集
液を供給し、二酸化炭素を捕集した捕集液８３を取り出
してガスクロマトグラフ６で二酸化炭素の測定を行う。
一方、酸素要求量測定部Ｂは、捕集槽８１内で発生した
二酸化炭素が捕集液８３内に吸収されるたことによる減
圧をマノメータ８７で検出し、電解制御部８９によって
電解槽８６を駆動して酸素を発生させ、培養槽８１内の
減圧を補償する構成であり、電解制御部８９において酸
素発生に要した電流を測定することによって酸素要求量
を測定する。The carbon gas measuring unit A is an oxygen demand measuring unit B.
The collection liquid is supplied from the collection liquid tank 3 into the culture tank 81 provided inside, and the collection liquid 83 that collects carbon dioxide is taken out and the carbon dioxide is measured by the gas chromatograph 6.
On the other hand, the oxygen demand measurement unit B detects the pressure reduction due to the absorption of the carbon dioxide generated in the collection tank 81 into the collection liquid 83 by the manometer 87, and the electrolysis control unit 89 controls the electrolysis tank 86. It is configured to drive and generate oxygen to compensate for the reduced pressure in the culture tank 81, and the oxygen demand is measured by measuring the current required for oxygen generation in the electrolysis control unit 89.
【００２４】図４に示す構成により、本発明の好気的生
分解度測定装置によって、二酸化炭素量に基づいた生分
解度の測定値と、従来の消費した酸素量により求める生
物化学的酸素要求量（ＢＯＤ値）との比較を行うことが
できる。With the configuration shown in FIG. 4, the aerobic biodegradability measuring apparatus of the present invention measures the biodegradability based on the amount of carbon dioxide and the biochemical oxygen demand obtained by the conventional amount of consumed oxygen. A comparison with the amount (BOD value) can be made.
【００２５】また、図５は、前記図３と図４に示した構
成例を組み合わせた構成を示し、複数個の培養槽８１を
備えた炭素ガス測定部Ａと、該培養槽８１の気相と連結
した電解槽８６およびマノメータ８７を備えた酸素要求
量測定部Ｂとを備えるとともに、捕集液の送液を行うペ
レスターポンプ４１，４２，〜４ｎを備えた部分と、培
養槽８１に捕集液を供給する捕集液タンク３，二酸化炭
素を吸収した捕集液を取り出して二酸化炭素を分離する
分離部５，分離した捕集液を廃液として溜める廃液タン
ク７，二酸化炭素の測定を行うガスクロマトグラフ６，
ペレスターポンプ４や酸素要求量測定部Ｂの制御を行う
制御部９，およびガスクロマトグラフ６で測定したデー
タ処理を行うデータ処理装置１０等を備えた部分とを含
んでいる。Further, FIG. 5 shows a constitution in which the constitution examples shown in FIG. 3 and FIG. 4 are combined, and a carbon gas measuring section A having a plurality of culture tanks 81 and a gas phase of the culture tank 81. An oxygen demand amount measuring unit B having an electrolyzer 86 and a manometer 87 connected to each other, and a portion equipped with the Pellester pumps 41, 42, to 4n for feeding the collected liquid, and the culture tank 81. Collection liquid tank for supplying collection liquid 3, Separation part for taking out collection liquid absorbing carbon dioxide and separating carbon dioxide 5, Waste liquid tank 7 for collecting separated collection liquid as waste liquid, measurement of carbon dioxide Gas Chromatograph 6
It includes a control unit 9 for controlling the Pellester pump 4 and the oxygen demand measurement unit B, and a unit including a data processing device 10 for processing the data measured by the gas chromatograph 6.
【００２６】制御部９はペレスターポンプ４や酸素要求
量測定部Ｂの制御を行ことにより、測定時期の制御や、
測定を行う培養槽の選択を行うことができ、これによっ
て、測定の自動化を行うことができる。The control unit 9 controls the perester pump 4 and the oxygen demand measuring unit B to control the measurement timing and
It is possible to select the culture tank in which the measurement is to be performed, which allows automation of the measurement.
【００２７】なお、複数個の培養槽の内の一つの培養槽
内の試料を既知の溶液として、基準の培養槽として使用
することもできる。本発明の実施の形態によれば、二酸
化炭素量に基づいて求めた生分解度によって、河川や排
水等の環境の汚染の程度や廃棄物処理の処理状況を測る
ことができ、また、微生物の生分解の活性度を測定する
ことができる。The sample in one of the plurality of culture tanks may be used as a known solution and used as a reference culture tank. According to the embodiment of the present invention, the degree of biodegradation obtained based on the amount of carbon dioxide can measure the degree of pollution of the environment such as rivers and drainage and the treatment status of waste treatment. The activity of biodegradation can be measured.
【００２８】[0028]
【発明の効果】以上説明したように、本発明によれば、
培養液中の微生物による有機物の分解の程度を表す好気
的生分解度を正確に求めることができる好気的生分解度
測定装置を提供することができる。As described above, according to the present invention,
It is possible to provide an aerobic biodegradation degree measuring device capable of accurately obtaining the degree of aerobic biodegradation, which indicates the degree of decomposition of organic matter by microorganisms in a culture solution.
【図１】本発明の装置の一構成例を説明するための図で
ある。FIG. 1 is a diagram for explaining a configuration example of an apparatus of the present invention.
【図２】本発明の装置によるガスクロマトグラフの測定
結果例である。FIG. 2 is an example of a measurement result of a gas chromatograph by the device of the present invention.
【図３】複数個の二酸化炭素捕集部を備えた好気的生分
解度測定装置の概略構成図である。FIG. 3 is a schematic configuration diagram of an aerobic biodegradation degree measuring device including a plurality of carbon dioxide traps.
【図４】好気的生分解度測定装置とＢＯＤ計との組み合
わせた概略構成図である。FIG. 4 is a schematic configuration diagram in which an aerobic biodegradability measuring device and a BOD meter are combined.
【図５】好気的生分解度測定装置とＢＯＤ計との組み合
わせた概略構成図である。FIG. 5 is a schematic configuration diagram in which an aerobic biodegradability measuring device and a BOD meter are combined.
【図６】従来から知られているＢＯＤ計の概略構成図で
ある。FIG. 6 is a schematic configuration diagram of a conventionally known BOD meter.
１，１１〜１ｎ…二酸化炭素捕集部、３…捕集液タン
ク、４，４１〜４ｎ…ペレスターポンプ、５…分離部、
６…ガスクロマトグラフ、７…廃液タンク、９…制御
部、１０…データ処理装置、２１，８１…培養槽、２
２，８２…捕集容器、２３、８３…捕集液、２４…培養
液、２５，８５…攪拌子、５１…分離容器、５２…中空
糸膜、５３…ガスサンプラー、５４…バルブ、８４…マ
グネットスターラー、８６…電解槽、８７…マノメー
タ、８８…エアータンク、８９…電解制御部、Ａ…炭酸
ガス測定部、Ｂ…酸素要求量測定部。1, 11-1n ... Carbon dioxide collecting part, 3 ... Collection liquid tank, 4, 41-4n ... Pellester pump, 5 ... Separation part,
6 ... Gas chromatograph, 7 ... Waste liquid tank, 9 ... Control part, 10 ... Data processing device 21, 81 ... Culture tank, 2
2, 82 ... Collection container, 23, 83 ... Collection liquid, 24 ... Culture liquid, 25, 85 ... Stirrer, 51 ... Separation container, 52 ... Hollow fiber membrane, 53 ... Gas sampler, 54 ... Valve, 84 ... Magnet stirrer, 86 ... Electrolyzer, 87 ... Manometer, 88 ... Air tank, 89 ... Electrolysis control section, A ... Carbon dioxide measuring section, B ... Oxygen demand measuring section.
───────────────────────────────────────────────────── フロントページの続き (72)発明者 松居 正巳 茨城県つくば市吾妻３−17−１ 株式会社 島津製作所つくば環境分析センター内 ─────────────────────────────────────────────────── ─── Continuation of the front page (72) Inventor Masami Matsui 3-17-1 Azuma Tsukuba, Ibaraki Shimadzu Corporation Tsukuba Environmental Analysis Center
Claims (1)
る二酸化炭素を捕集液内に捕集する二酸化炭素捕集部
と、前記捕集液から二酸化炭素を分離する分離部と、分
離した二酸化炭素を検出する二酸化炭素検出部と、少な
くとも二酸化炭素捕集部から分離部へ捕集液を送液する
送液部とを備え、検出した二酸化炭素量に基づいて生分
解度を測定することを特徴とする好気的生分解度測定装
置。1. A carbon dioxide collection part for collecting carbon dioxide generated by decomposition of organic matter by microorganisms in a collection liquid, a separation part for separating carbon dioxide from the collection liquid, and the separated carbon dioxide A carbon dioxide detecting unit for detecting and a liquid feeding unit for feeding the collected liquid to the separating unit from at least the carbon dioxide collecting unit, and the biodegradation degree is measured based on the detected carbon dioxide amount. Aerobic biodegradability measuring device.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP14262296A JP4025862B2 (en) | 1996-06-05 | 1996-06-05 | Aerobic biodegradation measuring device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP14262296A JP4025862B2 (en) | 1996-06-05 | 1996-06-05 | Aerobic biodegradation measuring device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH09325144A true JPH09325144A (en) | 1997-12-16 |
| JP4025862B2 JP4025862B2 (en) | 2007-12-26 |
Family
ID=15319629
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP14262296A Expired - Lifetime JP4025862B2 (en) | 1996-06-05 | 1996-06-05 | Aerobic biodegradation measuring device |
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| Country | Link |
|---|---|
| JP (1) | JP4025862B2 (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2006265070A (en) * | 2005-03-25 | 2006-10-05 | Hamamatsu Kagaku Gijutsu Kenkyu Shinkokai | Method and apparatus for judging degree of aging |
| KR20210111427A (en) * | 2020-03-03 | 2021-09-13 | (주)테크넬 | Compost maturity measuring device |
| CN113533683A (en) * | 2021-06-02 | 2021-10-22 | 广东新泓环境咨询有限公司 | Surface soil petroleum hydrocarbon pollution early warning method and system |
-
1996
- 1996-06-05 JP JP14262296A patent/JP4025862B2/en not_active Expired - Lifetime
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2006265070A (en) * | 2005-03-25 | 2006-10-05 | Hamamatsu Kagaku Gijutsu Kenkyu Shinkokai | Method and apparatus for judging degree of aging |
| JP4521841B2 (en) * | 2005-03-25 | 2010-08-11 | 国立大学法人静岡大学 | Determination of maturity |
| KR20210111427A (en) * | 2020-03-03 | 2021-09-13 | (주)테크넬 | Compost maturity measuring device |
| CN113533683A (en) * | 2021-06-02 | 2021-10-22 | 广东新泓环境咨询有限公司 | Surface soil petroleum hydrocarbon pollution early warning method and system |
Also Published As
| Publication number | Publication date |
|---|---|
| JP4025862B2 (en) | 2007-12-26 |
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