JPH09243636A - Analysis of substance present on skin - Google Patents
Analysis of substance present on skinInfo
- Publication number
- JPH09243636A JPH09243636A JP8078302A JP7830296A JPH09243636A JP H09243636 A JPH09243636 A JP H09243636A JP 8078302 A JP8078302 A JP 8078302A JP 7830296 A JP7830296 A JP 7830296A JP H09243636 A JPH09243636 A JP H09243636A
- Authority
- JP
- Japan
- Prior art keywords
- skin
- substance
- sample
- thermal decomposition
- analysis
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
Description
【0001】[0001]
【発明の属する技術分野】本発明は、皮膚表面、または
皮膚内部に存在する物質の分析方法、および皮膚外用剤
等の有効性の評価方法に関する。TECHNICAL FIELD The present invention relates to a method for analyzing a substance existing on the surface of the skin or inside the skin, and a method for evaluating the effectiveness of a skin external preparation or the like.
【0002】[0002]
【従来の技術】従来、皮膚外用剤等に配合されている紫
外線吸収剤、生理活性成分、防腐剤などの皮膚表面から
の経皮吸収性を評価する場合、フランツセルを用いた経
皮吸収性モデルを利用した方法、ラジオアイソトープを
用いた方法等が用いられてきた。しかしながら、これら
の方法は、いずれも動物を試験対象にしていたり、間接
的な評価方法であったりした為、人体の皮膚表面からの
経皮吸収性を正確に評価する点では問題があった。2. Description of the Related Art When evaluating the transdermal absorbability from the skin surface of ultraviolet absorbents, physiologically active ingredients, preservatives, etc., which have been conventionally compounded in external preparations for skin, etc., the transdermal absorbability using Franz cell is used. A method using a model, a method using a radioisotope, etc. have been used. However, all of these methods have been problematic in that the transdermal absorbability from the skin surface of the human body is accurately evaluated, because they are used as test subjects of animals or are indirect evaluation methods.
【0003】そして、人体で実際に試験した例として、
皮膚内部のセラミドを、皮膚から溶剤を用いて抽出し、
分析した例があるが、この方法では被検者に多大の負担
をかける他、経時での評価が実施しにくい問題があっ
た。And, as an example of actually tested on a human body,
Ceramide inside the skin is extracted from the skin with a solvent,
Although there are examples of analysis, this method has a problem in that it imposes a great burden on the subject and that evaluation over time is difficult to carry out.
【0004】[0004]
【発明が解決しようとする課題】従って、本発明の目的
としては、経皮吸収性などを直接に人体の皮膚で分析し
評価することが可能であること、その経時変化を分析し
評価することができること、分析方法が簡便であるこ
と、人体に有害な作用が及ばないこと、皮膚に存在する
多種の物質を定量できる分析方法であること、そして皮
膚外用剤等の有効性を評価する為に利用できる分析方法
を提供することを目的とする。Therefore, an object of the present invention is to be able to directly analyze and evaluate percutaneous absorbability and the like on human skin, and to analyze and evaluate its change over time. In order to evaluate the effectiveness of external preparations for skin, etc., that the analysis method is simple, that the harmful effect on the human body is not exerted, that many kinds of substances existing on the skin can be quantified The purpose is to provide an analytical method that can be used.
【0005】[0005]
【課題を解決するための手段】本発明人らはこれらの問
題に鑑み鋭意研究した結果、皮膚から粘着性テープ等に
より採取した試料をダブルショットパイロライザーを用
いた熱分解マススペクトル法、または熱脱着マススペク
トル法により皮膚に存在する特定物質の定量を行うこと
で、皮膚外用剤中の配合成分の経皮吸収性、または皮膚
外用剤等の効果による皮膚組織中の固有成分の変化量の
分析が行えることを見いだした。Means for Solving the Problems As a result of intensive studies in view of these problems, the present inventors have found that a sample collected from the skin with an adhesive tape or the like is subjected to a thermal decomposition mass spectrum method using a double shot pyrolyzer, or a thermal decomposition method. By quantifying specific substances present on the skin by desorption mass spectrometry, the percutaneous absorbability of the compounding ingredients in the external preparation for skin, or the analysis of the amount of change in the specific ingredient in skin tissue due to the effects of the external preparation for skin, etc. I found that I can do it.
【0006】即ち、本発明は、皮膚から採取した試料を
熱分解マススペクトル法、または熱脱着マススペクトル
法を用いて皮膚表面、または皮膚内部に存在する特定物
質の定量を行うことを特徴とする皮膚存在物質の分析方
法にある。That is, the present invention is characterized in that a sample collected from the skin is subjected to a thermal decomposition mass spectrum method or a thermal desorption mass spectrum method to quantify a specific substance present on the skin surface or inside the skin. It is in the analysis method of substances present in the skin.
【0007】そして、この分析方法が、有機系紫外線吸
収剤、防腐剤、酸化防止剤など、本来、皮膚内部へ経皮
吸収させたくない物質の経皮吸収を阻害する機能を有す
る皮膚外用剤の開発、セレブロシド、ビタミン類などの
生理活性成分の使用による皮膚状態の変化の経日観察、
および効果の評価判定、スクワレンなどを指標とした皮
脂分泌量の観察に基づいたメイクアップ化粧料の開発な
ど、従来分析ができないために明確な科学的根拠を持ち
にくかった皮膚外用剤の開発に極めて有効であることを
見いだした。[0007] This analysis method is applied to skin external preparations having a function of inhibiting the percutaneous absorption of substances which should not be percutaneously absorbed into the skin, such as organic UV absorbers, preservatives and antioxidants. Development, daily observation of changes in skin condition due to the use of physiologically active ingredients such as cerebroside and vitamins,
It is extremely useful for the development of external preparations for the skin that have a difficult scientific basis because conventional analysis cannot be performed, such as evaluation evaluation of effects and development of makeup cosmetics based on the observation of sebum secretion using squalene as an index. I found it to be effective.
【0008】[0008]
【発明の実施の形態】以下に本発明の構成を詳説する。
本発明で言う皮膚とは、ヒトの顔面、腕、頭皮等の全身
の皮膚を対象とし、また、兎、モルモット、鼠、犬、
豚、牛等の動物の全身の皮膚を対象とする。BEST MODE FOR CARRYING OUT THE INVENTION The constitution of the present invention will be described in detail below.
The skin referred to in the present invention is intended for the skin of the whole body such as human face, arms and scalp, and also rabbits, guinea pigs, rats, dogs,
The skin of the whole body of animals such as pigs and cows is targeted.
【0009】本発明で用いる試料の採取方法としては、
粘着性テープ、吸着紙、接着剤などを使用して皮膚から
試料を採取方法が挙げられるが、粘着性テープを用いる
方法が採取効率、剥離時の抵抗感、簡易性、再現性の点
から最も好ましい。採取方法の具体例としては、例えば
以下に挙げる方法が挙げられる。As a method of collecting a sample used in the present invention,
The method of collecting a sample from the skin using adhesive tape, suction paper, adhesive, etc. is the most suitable method from the viewpoint of collection efficiency, resistance to peeling, simplicity, and reproducibility. preferable. Specific examples of the collection method include the following methods.
【0010】(1)粘着性テープを用いる方法 粘着性テープを採取皮膚部位に張り、一定の圧力(例え
ば、ミクロスパーテルで数回擦るなど)を加えた後、粘
着性テープを剥し、試料を採取する。この時、測定試料
の大きさとしては、一辺2×2mmの方形あるいは直径
3mmの円形が好ましく用いられる。尚、粘着性テープ
としては、コスト、簡便性、粘着剤の均質性からセロフ
ァンテープを用いることが好ましい。(1) Method of using adhesive tape An adhesive tape is applied to the skin site, a certain pressure is applied (for example, rubbing with a microspatel several times), the adhesive tape is peeled off, and a sample is taken. To do. At this time, as the size of the measurement sample, a square having a side of 2 × 2 mm or a circle having a diameter of 3 mm is preferably used. As the adhesive tape, cellophane tape is preferably used in terms of cost, simplicity, and homogeneity of the adhesive.
【0011】(2)吸着紙を用いる方法 吸着紙を採取皮膚部位につけ、一定時間(例えば15分
間)、一定の圧力(例えば1kg/cm2 )を加えた
後、吸着紙を剥し、試料を採取する。尚、吸着紙として
は、濾紙や吸い取り紙等を用いることができる。(2) Method using adsorption paper Adhesion paper is applied to the skin site, and after applying a constant pressure (eg 1 kg / cm 2 ) for a certain period of time (eg 15 minutes), the adsorption paper is peeled off and a sample is collected To do. As the suction paper, filter paper, suction paper, or the like can be used.
【0012】(3)接着剤を用いる方法 接着剤を採取皮膚部位に塗布し、乾いた後に剥し、試料
を採取する。尚、接着剤としては、シアノアクリレート
系の接着剤等が好ましい。(3) Method Using Adhesive An adhesive is applied to the sampled skin site, dried and then peeled off to collect a sample. As the adhesive, a cyanoacrylate adhesive or the like is preferable.
【0013】(4)レプリカを用いる方法 市販のレプリカ剤(シリコーン系、エポキシ系、ポリウ
レタン系等)を塗布し、硬化後に剥し、試料を採取す
る。(4) Method using a replica A commercially available replica agent (silicone-based, epoxy-based, polyurethane-based, etc.) is applied and, after curing, peeled off and a sample is taken.
【0014】これらの採取方法が好ましい点としては、
採取部位が僅かで良いため、採取部位が大きい場合と比
べて、採取回数を増やすことが可能である点が挙げられ
る。採取回数を増やせると、経時変化の測定や部位別の
測定が容易となる利点がある。また、同一部位の採取回
数を変化させることにより、皮膚の深さ方向の情報も得
られる利点がある。As a preferable point of these sampling methods,
Since the number of collection sites is small, it is possible to increase the number of collections as compared with the case where the collection site is large. Increasing the number of collections has the advantage of facilitating the measurement of changes over time and site-specific measurements. Further, there is an advantage that information in the depth direction of the skin can be obtained by changing the number of times of sampling of the same site.
【0015】本発明で言う分析方法における試料の前処
理にあたる熱分解法とは、物質を高温で分解し、その分
解生成物により物質を定量分析する方法を指し、また、
熱脱着法とは、物質を低、中温で熱し、目的とする成分
を分解することなく気化させることにより物質を定量分
析する方法を指す。熱分解法、または熱脱着法の例とし
ては、例えばダブルショットパイロライザー(フロンテ
ィア ラボ社製)に代表される加熱炉型熱分解装置を用
い、熱分解・熱脱着部温度を室温〜800℃、好ましく
は300〜600℃で、インターフェイス部温度を室温
〜400℃、好ましくは250〜400℃に設定し分析
する方法が好ましい。この範囲であれば、目的とする成
分を熱分解または熱脱着により再現性よく定量すること
ができる。The thermal decomposition method, which is the pretreatment of the sample in the analysis method of the present invention, refers to a method of decomposing a substance at a high temperature and quantitatively analyzing the substance by the decomposition product.
The thermal desorption method refers to a method of quantitatively analyzing a substance by heating the substance at low and medium temperatures and vaporizing the target component without decomposing it. As an example of the thermal decomposition method or the thermal desorption method, for example, a heating furnace type thermal decomposition apparatus represented by a double shot pyrolyzer (manufactured by Frontier Lab) is used, and the thermal decomposition / thermal desorption section temperature is room temperature to 800 ° C. It is preferable to set the interface temperature at room temperature to 400 ° C., preferably 250 to 400 ° C. at 300 to 600 ° C., and analyze. Within this range, the target component can be quantified with good reproducibility by thermal decomposition or thermal desorption.
【0016】尚、採取した試料を溶剤抽出して特定物質
を分析する方法も考えられるが、この方法では、前処理
工程が煩雑となる欠点がある。Although a method of extracting a sample with a solvent to analyze a specific substance can be considered, this method has a drawback that the pretreatment step becomes complicated.
【0017】本発明で用いる試料の熱分解または熱脱着
後の検出手段としては、質量分析計(マススペクトル)
を用いる。本発明で用いる分離手段としては、直接、熱
分解・熱脱着装置と質量分析計を接続する方法や、熱分
解・熱脱着装置と質量分析計の間にガスクロマトグラフ
ィー、液体クロマトグラフィー等の分離装置を接続する
方法が挙げられる。As a detection means after thermal decomposition or thermal desorption of the sample used in the present invention, a mass spectrometer (mass spectrum) is used.
Is used. As the separation means used in the present invention, a method of directly connecting the thermal decomposition / thermal desorption device and the mass spectrometer, or separation of gas chromatography, liquid chromatography or the like between the thermal decomposition / thermal desorption device and the mass spectrometer. There is a method of connecting devices.
【0018】本発明で言う皮膚に存在する特定物質とし
ては、皮膚外用剤中の配合成分、皮膚組織中の固有成
分、経皮吸収促進剤の一種または二種以上が挙げられ
る。例えばスクワレン、有機系紫外線吸収剤、セラミ
ド、生理活性成分、防腐剤、酸化防止剤、香料、界面活
性剤、ヒアルロン酸等の保湿剤、ケラチン、油剤、経皮
吸収促進剤等が挙げられる。さらに、皮膚に影響を与え
る物質の例として、環境汚染物質、感作性物質なども挙
げることができる。Examples of the specific substance existing on the skin in the present invention include one or more of a compounding ingredient in an external preparation for skin, an intrinsic ingredient in skin tissue and a percutaneous absorption enhancer. Examples include squalene, organic ultraviolet absorbers, ceramides, physiologically active ingredients, preservatives, antioxidants, fragrances, surfactants, moisturizers such as hyaluronic acid, keratin, oils, and transdermal absorption enhancers. Furthermore, examples of substances that affect the skin include environmental pollutants and sensitizing substances.
【0019】本発明で言う測定部位の皮膚としては、表
皮、真皮と皮膚角層の表面を対象とする。The skin of the measurement site referred to in the present invention is the surface of the epidermis, dermis and the stratum corneum of the skin.
【0020】本発明の上記分析方法を用い有効性に優れ
た皮膚外用剤を開発することができる。即ち、本分析方
法により、各種成分の皮膚への経皮吸収性や皮脂量変
化、生体内物質の経時変化を分析し評価し、その結果に
基づき開発された、例えば経皮吸収性を阻害した皮膚外
用剤、経皮吸収性を促進した皮膚外用剤、皮脂量の変化
に対応した皮膚外用剤、皮脂量を抑制する皮膚外用剤、
生理活性成分の効果を効果的に実証した皮膚外用剤、さ
らには環境汚染物質より皮膚を保護する皮膚外用剤等を
提供できる。By using the above-mentioned analysis method of the present invention, a highly effective external preparation for skin can be developed. That is, according to this analysis method, percutaneous absorption of various components into the skin, changes in the amount of sebum, changes over time of substances in the body were analyzed and evaluated, and the results were developed based on the results, for example, percutaneous absorption was inhibited. Skin external preparation, skin external preparation that promotes percutaneous absorption, skin external preparation that responds to changes in the amount of sebum, skin external preparation that suppresses the amount of sebum,
It is possible to provide a skin external preparation that effectively demonstrates the effects of physiologically active ingredients, and a skin external preparation that protects the skin from environmental pollutants.
【0021】皮膚外用剤の例としては、軟膏、バップ
剤、育毛剤、発毛剤、サンスクリーン剤、ファンデーシ
ョン、白粉、口紅、アイシャドウ、チーク、化粧下地、
乳液、ローション、クリーム、エッセンス、クレンジン
グ、パック、シャンプー、リンス、入浴剤、香料、石
鹸、デオドラント剤等が挙げられる。Examples of the external preparation for skin include ointment, poultice, hair-growth agent, hair-growth agent, sunscreen agent, foundation, white powder, lipstick, eye shadow, cheek, makeup base,
Examples include emulsions, lotions, creams, essences, cleansing, packs, shampoos, rinses, bath agents, fragrances, soaps, deodorants and the like.
【0022】皮膚外用剤中の配合成分としては、前記物
質以外に、例えば通常医薬品や化粧料に用いられる油
剤、粉体(顔料、色素、樹脂)、フッ素化合物、樹脂、
界面活性剤、粘剤、防腐剤、香料、紫外線吸収剤(有機
系、無機系を含む。UV−A、Bのいずれに対応してい
ても構わない)、保湿剤、生理活性成分、塩類、溶媒、
酸化防止剤、キレート剤、中和剤、pH調整剤等の成分
が挙げられる。As the compounding ingredients in the external preparation for skin, in addition to the above-mentioned substances, for example, oil agents, powders (pigments, dyes, resins), fluorine compounds, resins, which are usually used in medicines and cosmetics,
Surfactants, adhesives, preservatives, fragrances, ultraviolet absorbers (including organic and inorganic ones, which may correspond to UV-A or B), moisturizers, physiologically active ingredients, salts, solvent,
Components such as an antioxidant, a chelating agent, a neutralizing agent, and a pH adjusting agent can be mentioned.
【0023】[0023]
【実施例】以下、実施例及び比較例によって本発明を詳
細に説明する。The present invention will be described in detail below with reference to examples and comparative examples.
【0024】実施例1 スクワレンの分析 本発明の方法により、皮膚外部に分泌された皮脂および
皮膚内部のスクワレン量の経時変化を分析した。実験方
法は以下の通りである。Example 1 Analysis of Squalene By the method of the present invention, changes over time in the amount of sebum secreted outside the skin and the amount of squalene inside the skin were analyzed. The experimental method is as follows.
【0025】(1)試料採取法 パネラー:女性10名 試料採取部位:顔面 頬部および額部 試料採取方法:市販のセロファンテープを用い、試料採
取部位にテープを圧着し、ミクロスパーテルにて3回擦
った後、テープを剥し、二つ折にする。このテープよ
り、直径3mmの円状に切り出したものを測定用試料と
した。この操作を、数回繰り返した。また、測定部位を
僅かに移動し、2、4、6時間後の測定を実施した。(1) Sampling method Panelists: 10 females Sampling site: face cheek and forehead Sampling method: Using commercially available cellophane tape, press the tape to the sampling site and use a microspatel 3 times. After rubbing, remove the tape and fold it in half. A circular sample having a diameter of 3 mm was cut out from this tape and used as a measurement sample. This operation was repeated several times. In addition, the measurement site was slightly moved, and the measurement was performed after 2, 4, and 6 hours.
【0026】(2)分析条件 測定は、熱分解・熱脱着装置からガスクロマトグラフ装
置を経由して、質量分析計により実施した。(2) Analytical conditions The measurement was carried out by a mass spectrometer from a thermal decomposition / thermal desorption device through a gas chromatograph.
【0027】熱分解・熱脱着装置(ダブルショットパ
イロライザー) 熱分解・熱脱着部温度:300℃ インターフェイス部温度:300℃ ガスクロマトグラフ装置(ヒューレットパッカード社
製) 注入口温度:300℃ 分離管温度:230℃(1min)→(35℃/mi
n)→300℃→(10℃/min)→350℃ 分離管:ジメチルポリシロキサン系カラム(ウルトラア
ロイUA−1(フロンティア ラボ社製)) 質量分析計:日本電子データム社製 JMS DX−
303 印加電圧:70eVThermal decomposition / thermal desorption device (double shot pyrolyzer) Thermal decomposition / thermal desorption part temperature: 300 ° C. Interface part temperature: 300 ° C. Gas chromatograph device (manufactured by Hewlett Packard) Injection port temperature: 300 ° C. Separation tube temperature: 230 ℃ (1min) → (35 ℃ / mi
n) → 300 ° C. → (10 ° C./min)→350° C. Separation tube: dimethyl polysiloxane-based column (Ultra Alloy UA-1 (manufactured by Frontier Laboratories)) Mass spectrometer: JMS DX-manufactured by JEOL Datum
303 Applied voltage: 70eV
【0028】(3)分析結果 分析結果の一例としてのスクワレンの経時変化を図1に
示す。(3) Analytical Results FIG. 1 shows the time-dependent change of squalene as an example of the analytical results.
【0029】尚、実施例1での剥したテープ表面を凍結
割断し、走査型電子顕微鏡観察し、皮膚表面と皮膚内部
の境界を設定し分析した。その結果、洗顔後、経時で皮
膚外部のスクワレン量の増加に伴い、皮膚内部のスクワ
レン量も増加すること、ファンデーション塗布時に皮脂
量が増加することなど、従来未知であったデータが得ら
れることが確認された。The surface of the peeled tape in Example 1 was freeze-fractured and observed by a scanning electron microscope, and the boundary between the skin surface and the inside of the skin was set and analyzed. As a result, after washing the face, with the increase in the amount of squalene outside the skin over time, the amount of squalene inside the skin also increases, the amount of sebum increases when applying the foundation, it is possible to obtain previously unknown data. confirmed.
【0030】実施例2 セラミドの分析 皮膚内部のセラミド量の変化を測定した。右外腕部をブ
ランクとし、左外腕部に市販のセレブロシド配合製剤を
1ケ月間塗布し、塗布0日目と1ケ月目の皮膚内部のセ
ラミド量を測定した。Example 2 Analysis of Ceramide The change in the amount of ceramide inside the skin was measured. With the right outer arm blank, a commercially available cerebroside-containing preparation was applied to the left outer arm for 1 month, and the amount of ceramide in the skin on the 0th and 1st month of application was measured.
【0031】(1)試料採取法 試料採取方法:市販のセロファンテープを用い、試料採
取部位にテープを圧着し、ミクロスパーテルにて3回擦
った後、テープを剥し、二つ折にする。このテープよ
り、直径3mmの円状に切り出したものを測定用試料と
した。この操作を、数回繰り返した。(1) Sampling method Sampling method: Using a commercially available cellophane tape, the tape was pressure-bonded to the sampling site, rubbed three times with a microspatel, and then the tape was peeled off and folded in half. A circular sample having a diameter of 3 mm was cut out from this tape and used as a measurement sample. This operation was repeated several times.
【0032】(2)分析条件 熱分解・熱脱着装置(ダブルショットパイロライザ
ー) 熱分解・熱脱着部温度:550℃ インターフェイス部温度:350℃ ガスクロマトグラフ装置(ヒューレットパッカード社
製) 注入口温度:300℃ 分離管温度:100℃→340℃ 分離管:ジメチルポリシロキサン系カラム 質量分析計:日本電子データム社製 JMS DX−
303 印加電圧:70eV(2) Analytical conditions Thermal decomposition / thermal desorption device (double shot pyrolyzer) Thermal decomposition / thermal desorption part temperature: 550 ° C. Interface part temperature: 350 ° C. Gas chromatograph device (manufactured by Hewlett Packard) Inlet temperature: 300 ℃ separation tube temperature: 100 ℃ → 340 ℃ separation tube: dimethylpolysiloxane-based column mass spectrometer: JEOL Datum JMS DX-
303 Applied voltage: 70eV
【0033】(3)分析結果 測定開始時、左右の皮膚内部のセラミド量に変化は認め
られなかった。1ケ月塗布後、セレブロシド塗布部位の
皮膚内部のセラミド量は未塗布部位の皮膚内部のセラミ
ド量と比べて有為に増加していることが認められた。(3) Analytical results At the start of the measurement, no change was observed in the amount of ceramide inside the left and right skin. After application for 1 month, the amount of ceramide inside the skin at the cerebroside-applied site was significantly increased compared to the amount of ceramide at the non-applied site.
【0034】実施例2の結果より、本発明の方法によ
り、人体を用いた皮膚組織内に存在する固有物質の量的
変化がより簡便に評価できることが確認された。From the results of Example 2, it was confirmed that the method of the present invention can more easily evaluate the quantitative change of the specific substance existing in the skin tissue using the human body.
【0035】実施例3 皮膚外用剤(ファンデーショ
ン)の評価 実施例1の測定方法により、ローションのみを塗布した
女性顔面の皮脂量を測定した結果、皮脂量が経時で増加
している例が多く見い出された。さらに、下記表1に示
す処方にて得られるファンデーションAを塗布し、これ
を実施例1と同様の方法により、皮脂量の評価を実施し
たところ、ローションのみの場合と比べて、さらに多く
の皮脂量が分泌されることを確認した。即ち、ファンデ
ーションAの塗布により、ファンデーションAの吸油量
以上に皮脂が分泌され、テカリ、崩れといった現象が発
生していることが判った。このことは、ファンデーショ
ンAの吸油量を増加させても化粧崩れを防げないことを
確認できた。Example 3 Evaluation of skin external preparation (foundation) As a result of measuring the amount of sebum on the female face to which only lotion was applied by the measuring method of Example 1, it was found that the amount of sebum increased with time. It was Furthermore, the foundation A obtained by the formulation shown in Table 1 below was applied, and the amount of sebum was evaluated by the same method as in Example 1. As a result, a larger amount of sebum was obtained as compared with the case of only the lotion. It was confirmed that the amount was secreted. That is, it was found that by applying the foundation A, sebum was secreted in excess of the oil absorption amount of the foundation A, and phenomena such as shine and collapse occurred. From this, it was confirmed that even if the oil absorption amount of Foundation A was increased, makeup breakdown could not be prevented.
【0036】そこで、フルオロアルキル・ポリオキシエ
チレン共変性シリコーン(以下、FPD変性シリコーン
と呼ぶ)を配合することでファンデーション自体の皮脂
耐久性を増加させた、表2に示す処方からなるファンデ
ーションBを作製し、実際に夏場(女性8名)に試用試
験を実施したところ、8名中全員に化粧崩れ、テカリが
認められず、この考え方が実証できた。Therefore, a foundation B having a formulation shown in Table 2 was prepared, in which the sebum durability of the foundation itself was increased by blending a fluoroalkyl / polyoxyethylene co-modified silicone (hereinafter referred to as FPD modified silicone). However, when a trial test was actually carried out in the summer (8 females), all of the 8 females did not have makeup makeup and no shininess, and this idea could be verified.
【0037】[0037]
【表1】 [Table 1]
【0038】(ファンデーションAの調製方法)表1の
全成分を均一に混合し、アトマイザーにて粉砕した後、
金型を用いて金皿に打型して製品を得た。(Preparation Method of Foundation A) After uniformly mixing all components shown in Table 1 and pulverizing with an atomizer,
The product was obtained by stamping on a metal plate using the mold.
【0039】[0039]
【表2】 [Table 2]
【0040】(ファンデーションBの調製方法)表2の
全成分を均一に混合し、アトマイザーにて粉砕した後、
金型を用いて金皿に打型して製品を得た。(Preparation Method of Foundation B) After uniformly mixing all components shown in Table 2 and pulverizing with an atomizer,
The product was obtained by stamping on a metal plate using the mold.
【0041】[0041]
【発明の効果】以上のことから、本発明は、皮膚から採
取した試料を熱分解マススペクトル法または熱脱着マス
スペクトル法を用いて特定物質の定量を行うことで、実
際に人体の皮膚での特定物質の経時変化を正確に、簡易
に分析し評価することができ、実用性に優れた皮膚存在
物質の分析方法を提供することができ、そして本発明の
方法を利用することにより、有効性が明確な皮膚外用剤
を開発することができる。As described above, according to the present invention, the amount of a specific substance is quantified by using a thermal decomposition mass spectrum method or a thermal desorption mass spectrum method on a sample collected from the skin, so that the skin of a human body is actually measured. Accurate, simple analysis and evaluation of changes over time of specific substances can be provided, and it is possible to provide a method for analyzing substances that are present in the skin that are highly practical, and by using the method of the present invention, the effectiveness is improved. It is possible to develop a clear external preparation for skin.
【図1】本発明の分析方法を用いた実施例1でのスクワ
レン量の経時変化の測定結果を示す。FIG. 1 shows the results of measurement of changes over time in the amount of squalene in Example 1 using the analysis method of the present invention.
───────────────────────────────────────────────────── フロントページの続き (51)Int.Cl.6 識別記号 庁内整理番号 FI 技術表示箇所 G01N 30/06 G01N 30/06 G 30/88 30/88 E (72)発明者 今関 雅文 神奈川県小田原市寿町5丁目3番28号 鐘 紡株式会社化粧品研究所内─────────────────────────────────────────────────── ─── Continuation of the front page (51) Int.Cl. 6 Identification code Internal reference number FI Technical display location G01N 30/06 G01N 30/06 G 30/88 30/88 E (72) Inventor Masafumi Imaseki Kanagawa 5-28, Kotobuki-cho, Odawara City, Kanebo Co., Ltd.
Claims (4)
クトル法、または熱脱着マススペクトル法を用いて皮膚
表面、または皮膚内部に存在する特定物質の定量を行う
ことを特徴とする皮膚存在物質の分析方法。1. A skin-existing substance characterized by quantifying a specific substance present on the skin surface or inside the skin by using a sample collected from the skin by a thermal decomposition mass spectrometry method or a thermal desorption mass spectrometry method. Analysis method.
プを使用することを特徴とする請求項1に記載の分析方
法。2. The analysis method according to claim 1, wherein an adhesive tape is used when collecting a sample from the skin.
用することを特徴とする請求項1、2に記載の分析方
法。3. The analysis method according to claim 1, wherein a heating furnace type thermal decomposition apparatus is used as the thermal decomposition method.
膚組織中の固有成分、経皮吸収剤からなる群から選ばれ
る一種または二種以上であることを特徴とする請求項1
〜3に記載の分析方法。4. The specific substance is one or more selected from the group consisting of a compounding ingredient in an external preparation for skin, an intrinsic ingredient in skin tissue, and a transdermal absorbent.
The analysis method according to any one of 3 to 3.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP8078302A JPH09243636A (en) | 1996-03-05 | 1996-03-05 | Analysis of substance present on skin |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP8078302A JPH09243636A (en) | 1996-03-05 | 1996-03-05 | Analysis of substance present on skin |
Publications (1)
Publication Number | Publication Date |
---|---|
JPH09243636A true JPH09243636A (en) | 1997-09-19 |
Family
ID=13658136
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP8078302A Pending JPH09243636A (en) | 1996-03-05 | 1996-03-05 | Analysis of substance present on skin |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPH09243636A (en) |
Cited By (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1008851A1 (en) * | 1998-12-11 | 2000-06-14 | Schnizer, Wolfgang, Prof. Dr. med. | Skin care system |
GB2352298A (en) * | 1999-07-21 | 2001-01-24 | Clariant Gmbh | Method of detecting organic substances on surfaces in humans. |
JP2003014762A (en) * | 2001-06-29 | 2003-01-15 | Sumitomo Chem Co Ltd | Skin sensitivity test method |
JP2009174867A (en) * | 2008-01-21 | 2009-08-06 | Shiseido Co Ltd | Evaluation method of skin quality using quantity ratio of soluble protein of horny layer protein as index |
JP2013015516A (en) * | 2011-06-07 | 2013-01-24 | Shiseido Co Ltd | Analysis method and analysis kit |
JP2014013201A (en) * | 2012-07-04 | 2014-01-23 | Shiseido Co Ltd | Apparatus, method, and program for visualizing ingredient distribution |
JP2014085292A (en) * | 2012-10-26 | 2014-05-12 | Kose Corp | In-skin behavior evaluation method |
JP2020508689A (en) * | 2017-03-03 | 2020-03-26 | チルドレンズ ホスピタル メディカル センター | Non-invasive method for skin sample collection and analysis |
-
1996
- 1996-03-05 JP JP8078302A patent/JPH09243636A/en active Pending
Cited By (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1008851A1 (en) * | 1998-12-11 | 2000-06-14 | Schnizer, Wolfgang, Prof. Dr. med. | Skin care system |
GB2352298A (en) * | 1999-07-21 | 2001-01-24 | Clariant Gmbh | Method of detecting organic substances on surfaces in humans. |
JP2003014762A (en) * | 2001-06-29 | 2003-01-15 | Sumitomo Chem Co Ltd | Skin sensitivity test method |
JP2009174867A (en) * | 2008-01-21 | 2009-08-06 | Shiseido Co Ltd | Evaluation method of skin quality using quantity ratio of soluble protein of horny layer protein as index |
JP2013015516A (en) * | 2011-06-07 | 2013-01-24 | Shiseido Co Ltd | Analysis method and analysis kit |
JP2014013201A (en) * | 2012-07-04 | 2014-01-23 | Shiseido Co Ltd | Apparatus, method, and program for visualizing ingredient distribution |
JP2014085292A (en) * | 2012-10-26 | 2014-05-12 | Kose Corp | In-skin behavior evaluation method |
JP2020508689A (en) * | 2017-03-03 | 2020-03-26 | チルドレンズ ホスピタル メディカル センター | Non-invasive method for skin sample collection and analysis |
US11911012B2 (en) | 2017-03-03 | 2024-02-27 | Children's Hospital Medical Center | Non-invasive methods for skin sample collection and analysis |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Kintz | Value of hair analysis in postmortem toxicology | |
KR100786030B1 (en) | A composition for scalp and hair comprising a substance having absorption through hair follicles and an ingredient for accelerating absorption through hair follicles | |
Rougier et al. | Regional variation in percutaneous absorption in man: measurement by the stripping method | |
Lotte et al. | Racial differences in the in vivo percutaneous absorption of some organic compounds: a comparison between black, Caucasian and Asian subjects | |
AU4960499A (en) | Non-invasive transdermal detection of analytes | |
CN110174488B (en) | Method for detecting allergen in cosmetics | |
Cooper | Anatomy and physiology of hair, and principles for its collection | |
JPH09243636A (en) | Analysis of substance present on skin | |
Horvath | Solubility of structurally complicated materials: 3. Hair | |
Tampucci et al. | Topical formulations containing finasteride. Part II: Determination of finasteride penetration into hair follicles using the differential stripping technique | |
Chèze et al. | Drugs involved in drug-facilitated crimes (DFC): analytical aspects: 2—Hair | |
Muizzuddin et al. | Effect of systemic hormomal cyclicity on skin | |
DE102016110474A1 (en) | PROFILE DIAGNOSTICS IN PERSONALIZED DERMATOLOGY, DERMATOPATHOLOGY AND COSMETICS | |
US20180190479A1 (en) | Molecular diagnostics in personalized dermatology, dermatopathology and cosmetics | |
Saxena et al. | Eyelid allergic contact dermatitis to black iron oxide | |
CN108885205A (en) | The evaluation method of the health of skin | |
Cho et al. | Molecular mapping of sebaceous squalene by ambient mass spectrometry | |
Vincenti et al. | New challenges and perspectives in hair analysis | |
Mendes et al. | Evaluation of hair fiber hydration by differential scanning calorimetry, gas chromatography, and sensory analysis | |
JP4113251B2 (en) | Hair absorption measurement method | |
Pandey et al. | Quantitative estimation of barbaloin in Aloe vera and its commercial formulations by using HPTLC | |
Zhai et al. | Efficacy of barrier creams (skin protective creams) | |
CN112294975A (en) | Contact allergen reagent combination and application thereof | |
CN100367923C (en) | Application of isostearyl alcohol as absorption promoting agent through hair | |
Chojnacka et al. | The Effect of Hair Characteristics and Treatments on Mineral Composition of Scalp Hair. |