JPH09239025A - Method for inactivating virus - Google Patents

Abstract

PROBLEM TO BE SOLVED: To inactivate or oxidize the microorganisms, such as bacteria and virus, existing in waterworks water, blood, plasma, serum components, sea water and other solns. or ammonia, bilirubin, poisons, etc. SOLUTION: The blood of the human body is subjected to extracorporeal circulation and voltages from 0.7V to 1.2V (VS, SCE) of the range where an electrolysis does not arise are applied on the blood under the extracorporeal circulation. The blood is passed between electrodes and electrodes to inactivate the viruses, such as HIV viruses and hepatitis viruses, existing in the blood and the plasma components, etc., by using the effect of the voltages and currents and thereafter, the blood is returned to the human body.

Description

Detailed Description of the Invention

[Field of Industrial Application] The present invention relates to a technique necessary for inactivating viruses such as HIV virus and hepatitis virus. TECHNICAL FIELD The present invention relates to a technique required for artificially activating or suppressing cells such as white blood cells, which have a role of regulating human immunity, to enhance or reduce human immunity.

[Prior Art] Conventionally, as a method for sterilizing or inactivating a virus, a drug or a vaccine is used to sterilize or inactivate the virus.
At present, no effective treatment method has been established for viruses such as V virus, hepatitis virus, and carcinogenic virus.

[Problems to be Solved by the Invention] Sterilization or inactivation or oxidation of bacteria, viruses and other microorganisms or ammonia, bilirubin, toxic substances and the like present in water and sewage water, blood, plasma, serum components or other solutions. The purpose is to let. The purpose of the present invention is to artificially activate or suppress cells such as white blood cells, which have a role of regulating human immunity, to enhance or reduce human immunity.

[Means for Solving the Problem] FIG. 1 is an explanatory view of the principle of the present invention. FIG. 1 is a longitudinal sectional view, in which 1 is a filtering instrument, 2 is an electrode, 3 is a terminal, Reference numeral 4 is a crosspiece, 5 is a filtering material or dielectric made of breathable conductive material, and 7 is a hole.

[Operation] An HIV virus in a free state existing in the blood or a voltage in the range that does not destroy important cells in blood, such as red blood cells, white blood cells or platelets, is used. Blood that inactivates or suppresses the HIV virus that is non-covalently bound to the surface of cells and that is circulated extracorporeally outside the human body is exchanged with cells such as red blood cells, white blood cells or platelets using a centrifuge. HIV virus that has been separated into plasma or serum components and transferred to plasma or serum components and then transferred to plasma or serum components using one or more breathable electrodes 6. Electrically inactivate.

[0006] As a means for uniformly applying an electric current in a solution, spherical particles or granules or porous or honeycomb shapes made of a conductor such as gold, platinum, carbon, graphite, activated carbon, titanium, palladium or nickel Alternatively, when a solution of an electrolyte such as blood or seawater is passed through the inside of the filtering device 1 which uses a sponge-shaped object or carbon fiber as the filtering material or the dielectric 5, without raising the temperature of the solution. However, even at low voltage, a large amount of negative electrode or positive electrode current can be flowed uniformly in a solution such as blood or seawater, so bacteria such as bacteria and viruses present in the solution, or ammonia, bilirubin, poisons, etc. It can be uniformly sterilized or inactivated or oxidized.

When an electric current is applied to blood or a solution of an electrolyte such as plasma, serum components or seawater from which blood is separated, an electrolyte solution without using a filter material or a dielectric 5 made of a gas permeable conductor. When an electric current is applied to the inside of the solution, an extremely thin passage through which the electric current flows is formed, and the electric current has the property of flowing only in the desired part of the solution. If the body 5 is not used, there is a problem that the current cannot be evenly flowed in the electrolyte solution.

[0008] Currently, as a means for adsorbing and removing ammonia, bilirubin, toxic substances, etc. in blood, a product having a product name of hemofilter or hemocolumn is used for therapy. This hemofilter or hemocolumn is made of activated carbon, which is made of activated carbon in the shape of a sphere with a diameter of about 0.5 mm, and the surface of particles made of activated carbon is coated with a biocompatible heparin coat that is compatible with living organisms. The particles are used as a filtering material or a dielectric 5, and ammonia, bilirubin, poisons and the like in blood are adsorbed and removed by the filtering material or the dielectric 5 made of activated carbon.

The present invention is used at both ends of the filter device 1 filled with the spherical filter material or the dielectric 5 made of activated carbon having a trade name of hemofilter or hemocolumn as described above. A spherical filter material or dielectric 5 made of activated carbon, which is provided with the terminal 3 and is filled inside the filter device 1.
As shown in FIG. 1 or FIG. 2, an electrode 2 or a gas permeable electrode 6 is provided so as to come into contact with the blood, and a spherical filter medium or dielectric 5 made of activated carbon is provided with blood or By passing an electric current of the negative electrode or the positive electrode within the range that does not affect the living body, the surface of the activated carbon is charged and an electrical oxidation action occurs, so that the action of oxidizing ammonia in blood to nitric acid is performed. Occurs.

When free HIV virus present in blood or infectious cells innumerably non-covalently bound to HIV virus are passed through the inside of the filtration device 1 shown in FIG. 1 or FIG. The spherical filter material or dielectric 5 made of activated carbon, which is filled in the inside of the filtering device 1,
When a DC or AC electric field is applied, the infected cells are repeatedly brought into contact with the charged filtering material or the dielectric 5 inside the filtration device 1, and the HI in a free state is repeated.
HI which is non-covalently bound to the V virus or cell metaphysis
V virus is an electrical influence, HIV virus RNA
Alternatively, the reverse transcriptase or proteolytic enzyme or the envelope on the surface of the HIV virus or other important part of the HIV virus is electrically destroyed.

Filter material or dielectric 5 made of breathable conductor
The spherical or porous or honeycomb-shaped or sponge-shaped filter material or the surface of the dielectric 5 is used as a biocompatible resin such as heparin coat, or an insulating resin. By using the filtering material or the dielectric material 5 made of an electric conductor, the surface property of which is reduced to reduce the conductive property of the filtering material or the dielectric material 5 made of an electric conductor. In addition, there is a characteristic that the charge capacity of the filtering material or the dielectric 5 made of a conductor is increased.

The blood of the human body is led out of the human body,
The blood circulating outside the body is molded from pure gold clay, pure silver clay or pure platinum clay manufactured by Mitsubishi Materials Corp., and the product is fired to form several microns to several tens of microns.
Using the breathable electrode 6 formed by forming the holes 7 in a material made of pure gold, pure silver, pure platinum clay, or the like having innumerable holes of about micron size and having a porous property similar to unglazed, Due to the synergistic effect of the hole 7 provided in the breathable electrode 6 and the hole having a porous property similar to unglazed, the effective surface area of the breathable electrode 6 is significantly increased.

Regarding the HIV virus and the electric field or current,
On November 26th, 1993 and February 16th, 1994, Professor Nagamoto of Nagasaki University, Professor Miyamoto, Assistant Professor Katamine, Graduate Student Taro Yamamoto and Hitachi Chemical Co., Ltd., and the applicant of the present invention, Nagaura. The experimental results of the joint research conducted with Yoshiaki revealed that the HIV or RNA or reverse transcriptase or proteolytic degradation of HIV virus was caused by the effect of electric field or current, the effect of electrolysis between electrodes, or other effects. Damage or destruction of the enzyme or the envelope on the surface of the HIV virus or other important parts of the HIV virus may result in inhibition of the HIV virus's ability to grow and inactivation.
It became clear from the following experimental results. In the case of 1 V, the HIV virus could be inactivated to about 1/10 or less when the distance between the electrodes was 0.3 mm and the electrification time was 17 minutes. In the case of 2 V, the distance between the electrodes was 1.0 mm, and the inactivation could be reduced to about ⅕ or less in 10 minutes.

From the above experimental results, it can be said that there is no correlation between the inactivation of HIV virus and the electric field strength, but there is a relation between electric field strength or other factors and time. Only that turned out at least. A report on HIV virus inactivation and electric field was reported in the report of the Ministry of Health, Labor and Welfare AIDS treatment method or prevention group in FY1993.
Although the report will be published by the Ministry of Health and Welfare, please refer to the outline of the report attached to the application documents.

The blood of the human body is circulated extracorporeally, and the blood circulated outside the body is circulated in FIGS. 3, 4, 5, 6, 8 and 1.
1, FIG. 14, FIG. 15, FIG. 16, FIG. 17, FIG.
9, FIG. 20, FIG. 21, FIG. 24, FIG. 33, FIG. 40, FIG.
As shown in FIG. 7, FIG. 48, FIG. 54, etc., by passing through the inside of the filtering instrument 1, the ammonia present in the blood is electrolyzed or the oxidation reaction of the electrodes is used, and a very low voltage is used. Then, ammonia is added to No, No ₂ , and No _3.
According to a joint study with Dr. Yasuhiro Yamada of the Kyushu Industrial Technology Research Institute, ammonia is finally transferred to nitric acid when it is oxidized by using the oxidation action of active oxygen that gradually occurs on the surface of the electrode. discovered. At the same time, it was discovered that when carbon is used as an electrode material, a carboxyl group is formed by the action of active oxygen generated on the electrode surface.

By utilizing the above-mentioned discovery of the oxidative action of ammonia, the treatment of acute liver failure or liver failure, which is now considered as an incurable disease, which occurs when ammonia cannot be decomposed in the liver As a method, ammonia dissolved in blood is oxidized using the oxidizing action of the electrode, and the toxicity is 1/10 to 1/2 that of ammonia.
It can be changed to nitric acid of 0 or less and excreted from the kidney of the human body, or can be changed to nitric acid that can be excreted from the human body by artificial dialysis using an artificial kidney.
An artificial liver organ functioning as an artificial liver can be produced, and it can be used as a method for treating acute liver failure or liver failure.

A large amount of electric current is applied to seawater to kill or inactivate microorganisms such as bacteria and viruses in seawater. When seafood or the like is bred or cultivated in seawater, no fish disease occurs. The state can suppress fish disease.

[Embodiment] An embodiment of the present invention will be described below with reference to FIG. FIG. 2 is a schematic structural explanatory view showing a virus inactivating means of the embodiment of the present invention. FIG. 2 is a longitudinal sectional view, in which 1 is a filtering instrument, 3 is a terminal, and 5 is a breathable conductive material. Filter material or dielectric made of body, 6 is a breathable electrode,
7 is a hole.

FIG. 2 shows that inside the filtering device 1, a filter material or a dielectric material made of a conductive material and having a spherical shape with a diameter of about several millimeters and made of conductive material is used. 5 is brought into contact with the breathable electrodes 6 provided above and below, and a negative or positive current is caused to flow through the filtering material or the dielectric 5 having a spherical shape made of a conductor, thereby making a breathable conductor. The blood of the human body is drawn out of the body of the filter device 1 in which the current of the negative electrode or the positive electrode is passed to the filter material or the dielectric 5 made of the above, and the plasma or serum component obtained by separating the blood from the whole blood. , Like the procedure of artificial dialysis, circulate extracorporeally inside the filtration device 1,
Blood or plasma or serum components of whole blood circulating extracorporeally,
HI in a free state existing in blood or plasma or serum components of whole blood by applying voltage or current inside the filtering instrument 1
V virus or H non-covalently bound to the surface of the cell
It shows a state in which the IV virus is electrically repelled, does not pass through the inside of the filter medium or the dielectric 5 made of breathable conductive material, is electrically adsorbed, or is sterilized or inactivated. ing.

The use of the above methods or means to oxidize the ammonia dissolved in the blood which occurs as a result of acute liver failure or liver failure using electrolysis or the oxidative action of the electrodes, Ammonia in blood can be oxidized to nitric acid.

An embodiment of the present invention will be described below with reference to FIG. FIG. 3 is a structural schematic explanatory view showing no means for inactivating the virus of the example of the present embodiment, and FIG. 3 is a vertical cross-sectional view.
1 is a filtering instrument, 3 is a terminal, 6 is a breathable electrode, 7 is a hole, 8 is an o-ring, and 9 is a packing.

FIG. 3 shows that the blood of the human body is drawn out of the body of the human body and the blood of the whole blood which is circulated extracorporeally, or the blood is subjected to a centrifugal separator to obtain red blood cells, white blood cells, platelets, etc. Cells and plasma or serum components,
After moving the HIV virus in the free state, which was present in the blood, into the plasma or serum component, the plasma or serum component containing the HIV virus is passed through the filtration device 1 provided with the breathable electrode 6. The HIV virus having an electric property which passes through the inside is electrically inactivated on the surface of the breathable electrode 6 and is contained in the blood or plasma of whole blood or the serum component. Circulating whole blood after inactivating or removing the HIV virus in the human body, or by combining plasma or serum components with cells such as red blood cells, white blood cells or platelets separated by a centrifuge, the human body It shows the state of reflux.

An embodiment of the present invention will be described below with reference to FIG. FIG. 4 is a structural schematic explanatory view showing no means for inactivating the virus of the example of the present embodiment, and FIG.
1 is a filtering instrument, 3 is a terminal, 6 is a breathable electrode, 7 is a hole, 8 is an o-ring, and 9 is a packing.

The air-permeable electrode 6 is shown in FIG.
Infected cells infected with HIV virus or HIV virus are used in the same manner as described with reference to FIG. 3 by using the filtration device 1 having four or more breathable electrodes. After inactivating or removing the HIV virus non-covalently bound to the surface of, the HIV virus in the blood is inactivated or removed, and the cleaned blood or plasma component of whole blood is returned to the human body. It shows the state.

An embodiment of the present invention will be described below with reference to FIG. FIG. 5 is a schematic structural explanatory view showing a means for inactivating a virus according to the embodiment of the present invention, and FIG.
1 is a filtering instrument, 3 is a terminal, 6 is a breathable electrode, and 7 is a hole.

The air-permeable electrode 6 is shown in FIG.
3 or 4 by using the filtration device 1 provided with one sheet.
HIV virus or H
It shows a state in which infected cells infected with IV virus are inactivated or removed.

An embodiment of the present invention will be described below with reference to FIG. FIG. 6 is a structural schematic explanatory view showing a means for inactivating a virus according to the embodiment of the present invention, and FIG. 6 is a vertical sectional view showing
1 is a filtering device, 3 is a terminal, 6 is a breathable electrode, 7 is a hole, and 9 is a packing.

FIG. 6 shows that the same as the breathable electrode 6 is provided inside the filtering instrument 1 so as to face the breathable electrode 6 and in the middle of another breathable electrode 6. Shape hole 7
The inside of the filtering device 1 in which the packing 9 made of an insulating material, which forms the above-mentioned structure, is sandwiched, is used in the same manner as described with reference to FIG.
After inactivating or removing the HIV virus that is non-covalently bound to the surface of infected cells infected with the virus, the HIV virus in the blood is inactivated or removed, and clean blood is returned to the human body. It shows the state.

In the case of the electrode structure shown in FIG. 6, the distance between the electrodes can be made extremely small, so that the potential gradient can be easily increased, which affects the living body. Even if the potential difference is 1 V, which is a voltage that does not apply a voltage, if the distance between the electrodes is 0.1 mm, the unit of the voltage applied to the object is 1 cm, so 1 cm / 0.1 mm = 100 times Therefore, even if the potential difference is 1 V, the distance between the electrodes is 0.1 m.
In the case of m, the influence of the voltage of 100V is exerted.

Shown in FIG. 7 is an enlarged view of the electrode structure shown in FIG. 6, in which the thickness of the hole 7 is larger than the thickness of the packing 9 which sandwiches the breathable electrode 6 in a sandwich shape. Since the major axis is large, the lines of electric force are generated in the direction of the arrow.
Since the line of electric force is generated in the direction of the arrow, the central portion of the hole 7 is less affected by the line of electric force. Therefore, it is more effective to reduce the major axis of the hole 7 formed in the breathable electrode 6 or the packing 9. Be correct.

An embodiment of the present invention will be described below with reference to FIG. FIG. 8 is a schematic structural explanatory view showing a means for inactivating a virus according to the embodiment of the present invention, and FIG. 8 is a vertical cross-sectional view.
1 is a filtering device, 3 is a terminal, 6 is a breathable electrode, 7 is a hole, 10 is an adhesive, 11 is a single-sided adhesive tape, 12
Is a double-sided adhesive tape, 13 is an insulating coating layer, 30 is a laminated electrode, and 32 is a packing with adhesive.

What is shown in FIG. 8 is different from that shown in FIG. 6 in that an adhesive 10 or a single-sided adhesive tape is used to bond two or more breathable electrodes 6 to each other. 11 or the double-sided adhesive tape 12, the insulating coating layer 13, or the packing 32 with an adhesive is used or formed to insulate the air-permeable electrode 6 and the air-permeable electrode 6 from each other, and The distance from the breathable electrode 6 can be made as small as possible, which is different from that of the laminated electrode 30 which has the highest potential gradient and has air permeability.

FIG. 9 is an enlarged view of the electrode structure shown in FIG. 8, in which the breathable electrode 6 and the breathable electrode 6 are bonded with an adhesive 10 or a single-sided adhesive tape 11 or double-sided adhesive. By using the tape 12, the insulating coating layer 13, the packing 32 with an adhesive, or the like, by stacking two or more breathable electrodes 6, the distance between the breathable electrodes 6 can be minimized. As in the case shown in FIG. 7, since the distance between the breathable electrodes 6 is smaller than the major axis of the hole 7, the line of electric force is the direction of the arrow. It shows the state that occurs in.

From the above, for example, when the distance between the breathable electrodes 6 is 1 mm, the voltage that does not affect the normal cells of the living body is around 2V. If the distance between the electrodes is 1 mm and a voltage of 2 V is applied to the HIV virus under exactly the same conditions, 90% or more of the HIV virus can be inactivated. However, when the distance between the electrodes is 10 mm and the voltage is 2 V, the HIV virus can hardly be inactivated.
For the purpose of completely inactivating the HIV virus, if a voltage of 2 V or more is applied with the distance between the electrodes being 10 mm, normal cells of the living body will be destroyed. The solution is to reduce the distance between the electrodes as much as possible to increase the potential gradient. For example, if the distance between the electrodes is 0.1 mm, then 2 V when the distance between the electrodes is 10 mm, the distance between the electrodes is 1/100.
2V when the distance between the electrodes is 0.1 mm has the same potential gradient as 200 V when the distance between the electrodes is 10 mm.

For example, if the distance between the electrodes is set to 0.1 mm, by applying a voltage of about 1 V, which does not affect normal cells of the living body and does not cause electrolysis, It is the same as the potential gradient of 100 V when the distance between the electrodes is 10 mm. The distance between the electrodes is extremely small, and the inside of the electrode structure with high potential gradient is
Since the actual voltage is IV even when the whole blood is passed, it exists in the blood without destroying cells such as red blood cells, white blood cells, and platelets, which are living cells, and without causing electrolysis. HIV viruses in a free state or non-covalently bound to the surface of infected cells can be selectively inactivated by utilizing the effect of voltage or current. This has been proved by joint research with a research team in the Department of Bacteriology, Nagasaki University. Some of them have been deleted due to space limitations, but
Please refer to the attached summary report.

An embodiment of the present invention will be described below with reference to FIG. FIG. 10 is a structural schematic explanatory view showing a means for inactivating a virus according to the embodiment of the present invention, and FIG. 10 is a longitudinal sectional view,
In the figure, 1 is a filtering instrument, 2 is an electrode, 3 is a terminal, 4 is a crosspiece, and 5 is a filtering material or dielectric made of a breathable conductor,
7 is a hole.

FIG. 10 shows the filtering device 1
The inside of the filter is filled with a filtering material or a dielectric 5 made of an electric conductor and having a spherical shape with a diameter of about several millimeters, which is made of an electric conductor and is provided inside the filtration device 1. A filter device in which two electrodes 2 are brought into contact with each other to cause an electric current to flow through a spherical filter material 5 made of an electric conductor and an electric current flow through a filter material or a dielectric 5 made of a gas permeable electric conductor. 1 shows a state in which exhaust gas discharged from an automobile engine or the like is passed through the inside of No. 1 to adsorb and remove nitrogen oxides or sulfur oxides or black smoke contained in the exhaust gas.

An embodiment of the present invention will be described below with reference to FIG. FIG. 11 is a structural outline explanatory view showing a virus inactivating means of the embodiment of the present invention. FIG. 11, FIG. 12 or FIG. 13 is a longitudinal sectional view, in which 1 is a filtering instrument and 3 is a terminal. Reference numeral 4 is a crosspiece, 5 is a filtering material or a dielectric made of a breathable conductor, 6 is a breathable electrode, and 7 is a hole.

FIG. 11 shows the filtering device 1
Inside, the two breathable electrodes 6 are provided facing each other, and inside the facing breathable electrodes 6 are made of a conductor, and are made of a conductor having a spherical shape with a diameter of about several millimeters. Filled with a filtering material or dielectric 5 and brought into contact with the opposing electrode 6,
A filtering device 1 in which an electric current is passed through a filtering material or a dielectric 5 having a spherical shape made of an electric conductor, and an electric current is passed through the air-permeable electrode 6 and a filtering material or a dielectric 5 made of an air-permeable conductor. Inside, the blood of the human body is guided in the direction of the arrow to the outside of the human body, and the blood of the whole blood or the plasma component that has separated the blood is passed through the extracorporeal circulation. The HIV virus contained therein is inactivated by the action of an electric field or an electric current and then returned to the human body.

When blood is passed through the inside of the filtration device 1 as shown in FIG. 11, it is non-covalently bound to the surface of HIV virus or cells contained in the blood in a free state. The existing HIV virus is inactivated or adsorbed by the influence of the potential gradient inside the filtration device 1.

As shown in FIG. 12 or FIG. 13, the potential gradient of the filter material or the dielectric 5 made of a gas permeable electric conductor contained in the filter device 1 is as shown in FIG. , The voltage is 0 in the center of the inside of the filtering device 1, and the potential gradient is + in the direction of the positive electrode from the part where the voltage of the center is 0 toward both ends of the electrode 6 having the terminals 3 at both ends.
The closer to 1, +2, +3 +4, +5 and the positive air-permeable electrode 6, the higher the voltage. Further, the voltage of the negative electrode becomes higher as it comes closer to the gas permeable electrode 6 of the negative electrode -1, -2, -3, -4, -5 toward the negative electrode.

An embodiment of the present invention will be described below with reference to FIG. FIG. 14 is a schematic structural explanatory view showing means for inactivating the virus of the example of the present embodiment, and FIG. 14 is a vertical sectional view,
In the figure, 1 is a filtering instrument, 2 is an electrode, 3 is a terminal, 4 is a crosspiece, and 5 is a filtering material or dielectric made of a breathable conductor,
7 is a hole.

FIG. 14 shows the filtering device 1
The breathable electrode 6 is provided inside, the two electrodes 2 are provided so as to face the breathable electrode 6, and the two electrodes 2 face each other.
The inside was filled with a filtering material or a dielectric material 5 made of a conductive material and having a spherical shape with a diameter of about several millimeters, which was made of a breathable conductive material, and was made of an electrode 2 and a breathable conductive material. Inside the filtering instrument 1 in contact with the filtering material or the dielectric 5, the blood of the human body is drawn out of the human body in the direction of the arrow, and the blood of the whole blood or the blood that has been extracorporeally circulated is separated. The figure shows a state in which the HIV virus contained in the blood or plasma component of whole blood is inactivated by the action of an electric field or an electric current after passing through the component, and then the blood is returned to the human body.

An embodiment of the present invention will be described below with reference to FIG. FIG. 15 is a structural schematic explanatory view showing the means for inactivating the virus of the example of the present embodiment, and FIG. 15 is a vertical sectional view,
In the figure, 1 is a filtering instrument, 2 is an electrode, 3 is a terminal, 4 is a crosspiece, and 5 is a filtering material or dielectric made of a breathable conductor,
7 is a hole.

FIG. 15 is different from the case shown in FIG. 14 in the following points. Two breathable electrodes 6 are provided to face each other inside and above the filtering instrument 1, and inside the facing breathable electrodes 6, a spherical shape made of a conductor and having a diameter of about several millimeters is provided. The filter material made of a conductive material or the dielectric material 5 is filled and brought into contact with the two air-permeable electrodes 6 provided on the upper and lower sides to form a spherical filter material made of a conductive material. An electric current is passed through the dielectric 5, and the filtering material or the dielectric 5 is made of the gas permeable electrode 6 and the gas permeable conductor.
In the inside of the filtering device 1 that is passing an electric current, the blood of the human body is guided from the direction of the arrow to the outside of the human body, and the blood of the whole blood which is circulated extracorporeally or the plasma component obtained by separating the blood is passed, HI contained in blood or plasma components of whole blood
It shows a state in which blood is circulated to the human body after the V virus is inactivated by the action of an electric field or an electric current.

An embodiment of the present invention will be described below with reference to FIG. FIG. 16 is a structural schematic explanatory view showing the means for inactivating the virus of the example of the present embodiment, and FIG. 16 is a vertical sectional view,
In the figure, 1 is a filtering instrument, 2 is an electrode, 3 is a terminal, 4 is a crosspiece, and 5 is a filtering material or dielectric made of a breathable conductor,
7 is a hole.

FIG. 16 shows the filtering device 1
Two electrodes 2 are provided facing each other inside, and inside the facing electrodes 2 are made of a conductive material, and are made of a breathable conductive material having a spherical shape with a diameter of about several millimeters. The filtering device 1 which is filled with the filtering material or the dielectric 5 and is in contact with the two electrodes 2 facing each other is used as the first electrode or the second electrode, or the filtering device 1 or the first electrode and the second electrode. A plurality of two electrodes, one pair of electrodes, are provided in series to form a laminated electrode. Inside the filtration device 1, the blood of the human body is drawn out of the human body in the direction of the arrow and circulated outside the body. The whole blood or the plasma component obtained by separating the blood is passed through to inactivate the HIV virus contained in the blood or plasma component of the whole blood by the action of an electric field or an electric current, and then returned to the human body. It shows the state of doing.

An embodiment of the present invention will be described below with reference to FIG. FIG. 17 is a structural schematic explanatory view showing the means for inactivating the virus of the example of the present embodiment, and FIG. 17 is a vertical sectional view,
In the figure, 1 is a filtering instrument, 2 is an electrode, 3 is a terminal, 4 is a crosspiece, and 5 is a filtering material or dielectric made of a breathable conductor,
7 is a hole.

FIG. 17 is different from the case shown in FIG. 16 in the following points. The air-permeable electrodes 6 are provided to face each other inside and above the filtering instrument 1, and the air-permeable electrodes 6 facing each other are filled with a filtering material or a dielectric 5 made of a conductor to form the gas-permeable electrodes 6 and 6. The filtration device 1 in contact with the filtration device 1 is used as a first electrode or a second electrode, or a plurality of two electrodes each having a pair of the first electrode and the second electrode as a pair are laminated in series. Inside the filtration device 1 provided with electrodes, the blood of the human body is led out from the body of the human body in the direction of the arrow, and the blood of the whole blood circulated extracorporeally or the plasma component obtained by separating the blood is passed. Shows that the HIV virus contained in the blood or plasma component of whole blood is inactivated by the action of an electric field or an electric current and then the blood is returned to the human body.

An embodiment of the present invention will be described below with reference to FIG. FIG. 18 is a schematic structural explanatory view showing a means for inactivating a virus according to the embodiment of the present invention, FIG. 18 is a three-dimensional view, and FIG. 19, FIG. 20, FIG. 21 or FIG.
Is a filtering device, 3 is a terminal, 7 is a hole, 8 is an O-ring, 9, 16, 17 or 18 is a packing, 14 or 15 is a lid, 19 is a bolt, and 20 is a connecting part. is there.

FIG. 18 shows the filtering device 1
A plurality of breathable electrodes 6 and a plurality of O-rings 8 or packings 9 are alternately laminated inside, and a bolt 19 for connecting the connection portion 20 and the terminal 3 provided on the breathable electrode 6 is used. A lid 1 that is fixed and is provided on both sides of the filtration device 1.
If the structure is to be sealed using the lid 4 or the lid 15, the packing 16, 17, or 18 is provided at each joint part to make the filtration device 1 capable of keeping the inside of the filtration device 1 completely airtight. The structure which can be shown is illustrated.

On November 26, 1993, a bacteriology of Nagasaki University was used on November 26, 1993, using a filtering instrument 1 having a structure similar to that shown in FIG. 18 and capable of maintaining a completely airtight state. Professor Miyamoto of the HIV virus research team in the classroom,
Associate Professor Katamine, Taro Yamamoto graduate student, Hitachi Chemical Co., Ltd.,
FIG. 3 is a three-dimensional view of the filtering device 1 when used in an experiment of joint research with Yoshiaki Nagaura, the applicant of the present invention. Regarding the experimental results, when the distance between the electrodes was 1 mm, the alternating current was 2 V, and the residence time was 10 minutes, about 80% of the HIV virus could be inactivated. The type A electrode structure described in the report attached to this application document is shown in FIG.
The structure is almost the same as that of the filtering device 1 shown in FIG.

FIG. 21 or FIG. 22 shows that
19 or 20 is different from that shown in FIG. 19 or 20 in that although FIG. 19 or 20 is provided with the lids 14 or 15 on both sides, only one side is shown in FIG. 20. The difference is that the filtering device 1 having a structure in which a lid 14 is provided on the inside is illustrated. When disassembling the filtering device 1, the lids 14 are provided on both sides.
It is more convenient to provide 15 or 15. Further, FIG.
9 or the packing 9 shown in FIG. 21 and FIG.
Alternatively, the thickness of the holes 7 or the number of the holes 7 is different from that of the packing 9 shown in FIG. The thickness of the holes 7 or the number of holes 7 of the backing 9 shown in FIG.
The thickness of the holes 7 provided in the breathable electrode 6 is exactly the same as the number of the holes 7.

An embodiment of the present invention will be described below with reference to FIG. FIG. 23 is a schematic structural view showing the means for inactivating the virus of the example of the present embodiment, and FIG. 23 or 28 is a three-dimensional view showing FIG. 24, FIG. 25, FIG. 26, FIG.
0, FIG. 31 or FIG. 32 is an assembly drawing, in which 1 is a filtering device, 6 is a breathable electrode, 7 is a hole, 8 is an O-ring,
9 is packing, 21 is breathable electrode with terminal, 22 or 23 is lid, 24 is bolt, 25 is nut, 26
Is a bolt.

FIG. 23 shows a plurality of sheets.
After the terminal breathable electrode 21 for wiring and a plurality of O-rings 8 or packing 9 are alternately laminated, and the terminal breathable electrode 21 and the O-rings 8 or packing 9 are alternately laminated one by one, A lid 22 or a lid 23 provided on both side surfaces of the breathable electrode 21 with terminals is replaced with a bolt 24 or a nut 25.
By sealing and forming the filtration device 1
The structure which can make the filter instrument 1 which can maintain a completely airtight state is illustrated.

24, 25, 26, 27 and 2
9, FIG. 30, FIG. 31, or FIG.
FIG. 24, FIG. 25, FIG. 2 in the assembly view of FIG.
6, FIG. 27, FIG. 29, FIG. 30, FIG. 31 or FIG. 32 is different only in the number of the holes 7 formed in the breathable electrode with terminal 21. Figure 30
In FIG. 26, only one hole 7 is shown, and in FIG. 26, FIG. 27, FIG. 31, or FIG. 32, the difference is that a plurality of holes 7 are formed. Further, FIG.
The packing 9 shown in FIG. 26, 29 or 31 and the packing 9 shown in FIG. 25, 27, 30 or 32 are different in the thickness of the holes 7 or the number of the holes 7. The thickness of the holes 7 of the packing 9 or the number of the holes 7 which are different from each other as shown in FIG. 27 or 32 are different from those of the holes 7 provided in the breathable electrode 6 and the breathable electrode 21 with terminals. The shape is exactly the same as the number of.

23, 24, 25, 26 or 2
The shape of the terminal 3 integrally formed with the breathable electrode with terminal 21 shown in FIG. 7 may be any shape, and as shown in FIG.
It may be directly connected to the breathable electrode 6.

On February 26, 1994, using a filtering instrument 1 having a structure similar to that shown in FIG. 23 or FIG. As explained in 18, we are Professor Miyamoto, Associate Professor Katamine, Taro Yamamoto graduate student of the HIV virus research team at Nagasaki University Bacteriology, Hitachi Chemical Co., Ltd., and the applicant of the present invention. FIG. 3 is a three-dimensional view of the filtration device 1 when used in an experiment of a joint research with Yoshiaki Nagaura. Regarding the experimental results, the distance between the electrodes is 0.1 mm, and the alternating current is 0.5 V,
When the residence time of the solution was 1 minute, about 20% of the HIV virus could be inactivated.

An embodiment of the present invention will be described below with reference to FIG. FIG. 33 is a structural outline explanatory view showing a virus inactivating means of the example of the present embodiment, FIG. 33 is a three-dimensional view, FIG. 34 is a top view, FIG. 35 is an assembly view, and FIG. 36 is a vertical sectional view. FIG. 37 is a three-dimensional sectional view, FIG. 38 is a vertical sectional view, and FIG.
9 is a three-dimensional sectional view, in which 1 is a filtering device, 3 is a terminal, 6 is a breathable electrode, 7 is a hole, 8 is an O-ring,
9, 16, 17 or 18 is a packing, 10 is an adhesive, 11 is a single-sided adhesive tape, 12 is a double-sided adhesive tape, 13 is an insulating coating layer, 30 is a laminated electrode, 31 is a temperature control tank. , 32 are packings with an adhesive, 33 is a connecting portion, and 37 is a bolt.

FIGS. 33 and 34 show that
A temperature control tank 31 is provided outside the filtration device 1, and in order to control the temperature inside the filtration device 1, cooling water or hot water is circulated inside the temperature control tank 31 to control the temperature inside the filtration device 1. The structure which can be shown is illustrated.

FIG. 35 shows the filtering device 1
36 is a vertical sectional view of the laminated electrode 30 used inside the filtration device 1, FIG. 37 is a three-dimensional sectional view of the laminated electrode 30, and FIG. 38 is a longitudinal sectional view of the laminated electrode 30. 39 is a three-dimensional cross-sectional view of the laminated electrode 30.

FIG. 36 shows that a plurality of air-permeable electrodes 6, the middle of the air-permeable electrodes 6 and the O-ring 8 or the packing 9 or the adhesive 10 or the single-sided adhesive. Using the tape 11, the double-sided adhesive tape 12, the insulating coating layer 13, the adhesive packing 32, the bolt 37, or the like, several to several tens or more breathable electrodes 6 are laminated to form a pair. The structure of the laminated electrode 30 is shown.

The difference between FIGS. 36 and 37 and FIGS. 38 and 39 is that in the laminated electrode 30 shown in FIGS. 36 and 37, the solution flowing inside the laminated electrode 30 does not stagnant in a straight line state. While flowing straight inside the laminated electrode 30, the laminated electrode 30 shown in FIG. 38 and FIG. 39 flows the inside of the laminated electrode 30 because the breathable electrodes 6 are alternately laminated. The structure is different in that the solution flows meandering inside the laminated electrode 30.

As shown in FIG. 36, FIG. 37, FIG. 38, and FIG. 39, the air-permeable electrode 6 is attached to the O-ring 8 or the packing 9 or the adhesive 9 to several tens or more. Using the adhesive 10, the double-sided adhesive tape 12, the packing 32 with adhesive, the bolt 37, or the like, the laminated electrode 30
If it is formed, there are the following advantages. The distance between the electrodes can be taken accurately. The solution flowing inside the laminated electrode 30 can be made to flow straight inside the laminated electrode 30 without stagnation, or the breathable electrodes 6 can be alternately laminated to form a laminated electrode 30.
It is also easy to allow the solution flowing inside to flow while meandering inside the laminated electrode. As shown in the assembly view of FIG. 35, the vertical sectional view of FIG. 41, and the assembly view of FIG. 42, when the laminated electrode 30 is used as the electrode inside the filtration device 1, the lids on both side surfaces of the filtration device 1 are used. 14 and the lid 15 or the packing 17 provided on the lid 14 on one side is used from both sides or one side to easily seal the inside of the laminated electrode 30 and maintain the airtight state inside the laminated electrode 30. Therefore, the terminal 3 and the connection portion 33 connected to the terminal 3 formed on the laminated electrode 30 can be formed.
However, due to water leakage of the solution, the breathable electrode 6 forming the laminated electrode 30 will not cause a short circuit between the upper and lower sides. As shown in the assembly drawings of FIGS. 35 and 42,
Assembly is facilitated by using the cartridge-type laminated electrode 30 that is easy to handle.

An embodiment of the present invention will be described below with reference to FIG. FIG. 40 is a structural outline explanatory view showing a virus inactivating means of the embodiment of the present invention, FIG. 40 is a three-dimensional view, FIG. 41 is a vertical sectional view, FIG. 42 is an assembly drawing, and FIG. 43 is a vertical sectional view. In the figure, FIG. 44 is a three-dimensional cross-sectional view, FIG. 45 is a vertical cross-sectional view, FIG. 46 is a three-dimensional cross-sectional view, in which 1 is a filtering instrument, 3 is a terminal, 6 is a breathable electrode, and 7 is Hole, 8 is o-ring,
9, 16, 17, and 18 are packings, 10 is an adhesive, 11 is a single-sided adhesive tape, 12 is a double-sided adhesive tape, 13 is an insulating coating layer, 30 is a laminated electrode, 31 is a temperature control tank. , 32 are packings with adhesive, 33 and 3
Reference numeral 5 is a connecting portion, 34 is an electrode connecting component, and 37 is a bolt.

41, 42, 43, 44 and 45.
And, as shown in FIG. 46, the breathable electrode 6 is used by using an O-ring 8 or packing 9, adhesive 10 or double-sided adhesive tape 12, insulating coating layer 13, adhesive packing 32 or bolt 37. Then, after forming the laminated electrode 30, the air-permeable electrodes 6 are connected by using the electrode connecting component 34.
Since the laminated electrode 30 and the terminal 3 can be connected by providing only one connecting portion 35 to be connected to the terminal 3 on the terminal 3, the assembly becomes easier than the case shown in FIG. There is. Other functions and effects are the same as those described with reference to FIGS. 33, 34, 35, 36, 37, 38 and 39.

An embodiment of the present invention will be described below with reference to FIG. FIG. 47 is a structural outline explanatory view showing a virus inactivating means of the example of the present embodiment, FIGS. 47 and 48 are three-dimensional views, FIG. 49 is a top view, FIG. 50 is a vertical sectional view, and FIG.
1, 52 and 53 are assembly drawings, in which 1 is a filtering instrument, 9 is a packing, 27 is an electrode with a connecting portion, 28
Is a connecting portion, 29 is a serpentine packing, 31 is a temperature control tank, and 36 is a joining part.

FIG. 47 shows the filtering device 1
Inside, a plurality of electrodes 27 with connecting portions and packing 9 or a plurality of packings 9 and serpentine packings 29 are provided in parallel with the longitudinal direction of the flow of the solution, with electrodes 27 with connecting portions and packings 9 or packing 9 and serpentine packings 29. The structure of the filtering instrument 1 which laminated | stacked several sheets is shown.

FIG. 48 shows that cooling tanks are provided on both sides of the filtering device 1 shown in FIG.
The structure which can circulate cooling water or warm water in the temperature control of the electrode 27 with a connection part and the temperature control tank 31, and can temperature-control the inside of the filtering instrument 1 is shown in figure.

FIG. 49 is a top view of the filtering device 1 shown in FIG. 48, showing a structure in which the temperature control tanks 31 are formed on both sides of the filtering device 1. Shows.

FIG. 50 shows the filtering device 1
The electrode 27 with a connecting portion, the packing 9, and the serpentine packing 29, which are the constituent components of the above, are illustrated.

FIG. 51 shows the filtering device 1
The assembly drawing of the filtration instrument 1 which uses the electrode 27 with a connection part and the serpentine type | mold packing 29 which are the components which comprise is shown in figure.

FIG. 52 shows the filtering device 1
Inside, a plurality of electrodes 27 with connecting portions, a plurality of serpentine packings 29, and a plurality of packings 9 are laminated, and a plurality of electrodes 27 with connecting portions, a plurality of serpentine packings 29, and a plurality of packings 9 are laminated in parallel with the longitudinal direction of the solution flow. FIG. 3 is an assembly diagram of the filtering device 1, illustrating that the contact area of the solution in contact with the electrode 27 with the connecting portion inside the filtering device 1 is enlarged by using the serpentine type packing 29.

FIG. 53 shows an assembly drawing of the filtering instrument 1 using only the electrode 27 with connecting portion and the packing 9.

As shown in FIG. 47, the filtering device 1 in which the connecting portion-equipped electrodes 27 are arranged in the vertical direction and which can maintain a completely airtight state is used in FIG. 18 and FIG. 23, 1994, as described in 23.
According to the result of an experiment conducted on 26th of March with the HIV virus research team in the Department of Bacteriology, Nagasaki University, the distance between the electrodes was 0.3 mm, the alternating current was 1 V, and the residence time of the solution was 17 minutes. In the case of, it was possible to inactivate about 90% of HIV virus. The type B electrode in the attached report has an electrode structure that is configured by using two electrodes 27 with joint portions and one serpentine type packing 29 and one packing 9 shown in the figure. Although it is slightly different from the content of the drawing shown in FIG. 47, it basically has the same shape as the type B described in the report.

Using the above-described two types of filtration equipment 1 and the filtration equipment 1 having three types of electrode structures of the filtration equipment 1 in which the electrodes 27 with connecting portions are arranged in the vertical direction, HIV virus, voltage and current The following is a summary of the results of the experiment conducted in collaboration with the HIV virus research team in the Department of Bacteriology, Nagasaki University. By making the distance between the electrodes as small as possible and increasing the potential gradient, it is possible to inactivate the HIV virus even in a solution of the electrolyte, using a voltage and current within a range that does not cause electrolysis. The HIV virus is inactivated in proportion to the voltage gradient. The reason why the HIV virus is inactivated is that the HIV virus is RNA or reverse transcriptase or proteolytic enzyme or HI.
Because the envelope or other important part on the surface of V virus is destroyed or damaged by the influence of voltage and current, or chlorine, active oxygen or carboxyl group generated by electrolysis, the HIV virus is Be inactivated. If the potential gradient is high, the HIV virus can be inactivated even at about 1 V. Therefore, by applying a voltage and a current to the blood circulating outside the body, the HIV virus in the blood can be inactivated. Can be inactivated. When the voltage is 1.5 V or higher, the effect of inactivating the HIV virus is reduced because bubbles are generated on the electrode surface and a polarization action occurs, and thus the effective surface area of the electrode is reduced. Although the apparent voltage is high, the actual voltage is low. Therefore, if the voltage is 1.5 V or higher, the effect of inactivating the HIV virus will decrease, so the distance between the electrodes should be as small as possible. do it,
Even with a voltage of about 1.5 V, it is necessary to increase the potential gradient as much as possible.

An embodiment of the present invention will be described below with reference to FIG. FIG. 54 is a structural outline explanatory view showing a virus inactivating means of the example of the present embodiment, FIGS. 54 and 55 are three-dimensional views, and FIGS. 56, 65, 66 and 67 are top views. 57, FIG. 58, FIG. 59, FIG. 60, FIG.
63 and 64 are longitudinal sectional views, FIG. 68 is a side view, and in the drawings, 1 is a filtering instrument, 7 is a hole, 31 is a temperature control tank,
38 is a cylindrical electrode, 39 is a cylindrical electrode, 40 and 41 are lids, 42, 43 and 44 are bolts, 45
And 46 are terminals, 47, 48, 49, 50 and 51 are packings, 52 is a sleeve provided with a groove 53, 53
Is a groove, 54 is a sleeve provided with holes 7, 55 is a sleeve, 56 is a thermometer, 57 is a spring, and 58 is a joint portion.

FIG. 54 shows that a cylindrical electrode 39 having a diameter smaller than that of the cylindrical electrode 38 is inserted into the cylindrical electrode 38 and fixed by using a bolt 42. The lid 40 made of chemical products or insulators
1 and 41 show the structure of a filter device 1 having a cylindrical shape, which is hermetically formed by using bolts 43 and 44 or a joint portion 58.

FIG. 55 shows that a temperature control tank 31 is provided on the outer wall of the filtering device 1 shown in FIG. 54 and a cylindrical electrode 38 and a cylindrical electrode 39 are used. In order to control the temperature inside the burned electrode, the temperature control tank 3
1 shows a state in which cooling water or warm water is circulated inside 1 to keep the temperature of blood, plasma component and lymph passing through the inside of the filtering device 1 at a constant temperature, for example, around 37 ° C. Shows.

FIG. 56 is a top view of the filtering device 1 shown in FIG. 55, in which the cylindrical electrode 38
The structure in which the temperature control tank 31 is formed on the outer wall of FIG. As shown in FIG. 60, the temperature adjusting tank 31 may not be provided on the outer wall of the cylindrical electrode 38.

57, 58, 59, 60 and 6
1, FIG. 62, FIG. 63 and FIG.
Inside the cylindrical electrode 38, another cylindrical electrode 39 having a smaller diameter than the cylindrical electrode 38, a sleeve 52 provided with a groove 53 made of a chemical product or an insulator, or a sleeve 54 provided with a hole 7 is formed. Alternatively, by using the sleeve 55, the cylindrical electrode 39 can be fixed accurately, and at the same time, the blood of the human body can be passed through the extracorporeally circulating solution such as blood, plasma components and lymph fluid without stagnation. 1 shows a vertical cross-sectional view of the filtering device 1, which has been performed.

FIG. 59 and FIG. 60 show that
Inside the cylindrical electrode 38, another cylindrical electrode 39 having a smaller diameter than that of the cylindrical electrode 38 is inserted, and the sleeve 5 provided with the groove 53 shown in FIG. 63 from both side surfaces.
2 is inserted, and the cylindrical electrode 39 is connected to the cylindrical electrode 38.
The lid 40 and the lid 4 which are accurately fixed to the central portion of the inside of the cylindrical electrode 38 and form inner screws from both side surfaces.
Figure 1 shows a longitudinal cross-section of a filtering device 1 of a cylindrical electrode with a sealed and cylindrical structure using 1.

In FIG. 61, another cylindrical electrode 39 having a diameter smaller than that of the cylindrical electrode 38 is inserted into the cylindrical electrode 38, and both side surfaces of FIG.
, The cylindrical electrode 39 is accurately fixed inside the cylindrical electrode 38 by inserting the sleeve 54 provided with the hole 7, and the lid 40 and the lid 41 are used from both sides. 1 shows a longitudinal sectional view of a filtering device 1 having a closed cylindrical shape.

In FIG. 62, another cylindrical electrode 39 having a hole 7 having a smaller diameter than that of the cylindrical electrode 38 is inserted into the cylindrical electrode 38, and both side surfaces are inserted. 66, by inserting the sleeve 55, the cylindrical electrode 39 having the hole 7 is accurately fixed inside the cylindrical electrode 38, and the lid 40 and the lid 41 are used from both side surfaces. FIG. 1 shows a vertical cross-sectional view of the filtering device 1 having a cylindrical structure that is sealed.

FIG. 63 shows another cylindrical electrode 39 in which a groove 53 is provided inside the cylindrical electrode 38.
66, the sleeve 55 shown in FIG. 66 is inserted from both sides, and the columnar electrode 39 having the groove 53 is accurately fixed inside the cylindrical electrode 38 and the lid is attached from both sides. A vertical cross-sectional view of the filtering device 1 is shown, which uses a 40 and a lid 41 and has a sealed cylindrical shape.

FIG. 64 shows that another cylindrical electrode 39 is provided inside a cylindrical electrode 38 provided with a groove 53.
65, the sleeve 53 shown in FIG. 65 is inserted from both sides, and the cylindrical electrode 39 is accurately inserted into the groove 5
3 is a longitudinal cross-sectional view of the filtering device 1 having a cylindrical structure which is fixed inside the cylindrical electrode 38 provided with 3 and is sealed by using the lid 40 and the lid 41 from both side surfaces.

FIG. 68 shows the sleeve 52 provided with the groove 53 or the sleeve 54 or sleeve 55 provided with the hole 7 shown in FIGS. 65, 66 and 67.
FIG.

54 and 55, the cylindrical electrode 39 is formed by inserting the cylindrical electrode 39 inside the cylindrical electrode 38. In the case of the filtering device 1 having a cylindrical electrode structure, the cylindrical electrode 39 will be described. Since blood passes through the entire outer circumference of the electrode, the distance between the electrodes is limited to the maximum in order to increase the potential gradient, for example, the distance between the electrodes of the cylindrical electrode 38 and the cylindrical electrode 39 is reduced to about 0.3 mm. Even if you do not apply unnecessary pressure to the blood that is circulating outside the body,
Since blood will pass through the entire outer peripheral surface of the cylindrical electrode 39, blood can be passed through the inside of the filtering device 1 smoothly.

An embodiment of the present invention will be described below with reference to FIG. 69 is a structural schematic explanatory view showing a means for inactivating a virus according to the example of the present embodiment, FIGS. 69 and 71 are vertical sectional views, FIG. 70 and FIG. 72 are three-dimensional views, and 1 in the drawing is filtration. Equipment, 2 is a breathable metal electrode, 4 is a crosspiece, 5 is a filtering material or a dielectric, 6 is a power feeding anode or a power feeding cathode, 4
0 and 41 are lids.

FIGS. 69 and 70 show that
A plurality of porous or spherical or sponge-shaped filter media or dielectrics 5 are placed in a DC electric field and are provided at both ends of a flat plate, an extract band mesh, a perforated blade, or other porous plate member for power supply. A DC voltage or an AC voltage is applied between the anode 6 for power supply and the cathode 6 for power supply to polarize the filtering material or the dielectric 5 and form an anode and a cathode at one end and the other end of the filtering material or the dielectric 5, respectively. Inside the filtration device 1 containing the three-dimensional electrode, the blood of whole blood containing cell parts such as red blood cells and white blood cells or the plasma component obtained by separating the blood of whole blood is passed,
Inactivates HIV virus, hepatitis virus, adult leukemia virus that is non-covalently bound to the surface of cells, or HIV virus, hepatitis virus, adult leukemia virus or other viruses that are free floating in solution The state is illustrated.

71 and 72 show that when a carbonaceous material such as activated carbon, graphite or carbon fiber is used as the filtering material or the dielectric 5, when oxygen gas is generated from the anode, The filtering material or the dielectric 5 is oxidized by oxygen gas and easily dissolved as carbon dioxide gas. In order to prevent this, the side of the filter material or the dielectric 5 which is to be positively polarized is coated with a platinum group metal oxide such as iridium oxide, ruthenium oxide or platinum on a base material such as titanium and is usually used as an insoluble metal electrode 2. 69 and a porous material is placed in contact with the filter material or the dielectric 5 in a contact state so that oxygen generation mainly occurs on the porous insoluble metal electrode 2. 70 shows a state different from the case shown in FIG.

FIG. 3, FIG. 4, FIG.
6, FIG. 7, FIG. 8, FIG. 11, FIG. 14, FIG. 15, FIG.
An electrode having the electrode structure shown in FIGS. 17, 18, 19, 20, 20, 21, 24, 33, 40, 47, 48, 54, 69 and 71 is used. Even Fig. 2
In the same manner as described in Section 1, the ammonia in the blood is converted to nitric acid by using the electrolysis of the ammonia dissolved in the blood, which occurs as a result of acute liver failure and liver failure, or the oxidation by the electrode action. It can be oxidized.

[Effects of the Invention] When a voltage or current is applied to a cell, the cell surface is momentarily released by stimulating a change in ion channel or membrane potential on the cell surface or a pump on the cell surface. Can change the ion channel of the cell or the membrane potential or the pump on the cell surface,
By using electric current stimulation to release, the solution outside the cell and the solution inside the cell come and go, for example, Na ions outside the cell flow into the cell, When the internal K ions flow out of the cell and the cell is activated, the cell produces and releases the satocaines interleukin and interferon outside the cell. As a result, interleukins and interferons, which are protective substances of the human body, are synthesized in the body.

Although the mechanism of action of interleukins and interferons against HIV virus, hepatitis virus and other viruses has not yet been fully elucidated, cells in which the interleukins and interferons bind to receptors on target cells and receive the information Becomes an antiviral state and inhibits the growth of the virus,
It will suppress the onset of IV virus, hepatitis virus and other viral diseases.

Sandwich shape in which an insulating layer is formed by using an O-ring or packing made of a breathable electrode and an insulator, an adhesive, a single-sided adhesive tape, a double-sided adhesive tape, an insulating coating layer, or a packing with an adhesive. In the case of a filtration device that uses a breathable, laminated electrode structure, the distance between the electrodes can be made extremely small to any distance, and the potential gradient can be easily increased. Therefore, it is possible to inactivate a virus such as HIV virus, hepatitis virus or carcinogenic virus by using a voltage within a range that does not affect the living body.

For example, the HIV virus can be inactivated at a voltage of about 60 V in the case of an alternating current when the distance between electrodes is 1 cm, which is a unit of electricity. The limit which can withstand is AC or DC, 3V is the limit. As a result, the potential gradient between 60V when the distance between electrodes is 1 cm and 3V when the distance between electrodes is 0.5 mm is the same. Therefore, if the voltage between electrodes is 0.5 mm and a voltage of 3 V is applied, the same effect as 60 V when the distance between electrodes is 1 cm is produced. From this, it is necessary to reduce the distance between the electrodes as much as possible because the limit of the cells of the living body that can withstand the voltage is about 3V. By using the present invention, even if the distance between the electrodes is made small, a large amount of solution such as blood can pass through, and at the same time, the current is not short-circuited and the voltage can be safely applied to the solution such as blood. Can be applied. However, if a high-voltage pulse is used, a higher voltage can be applied to the blood, and therefore it can be said that the high-voltage pulse is more effective.

According to the results of an experiment conducted on June 14, 1993, in collaboration with Dr. Hiroshi Shiraki, Director of the Fukuoka Blood Center, the same filtering device as shown in FIG. 18 was used. Then, an experiment was conducted on how much V the blood can withstand the effects of chlorine, active oxygen, carboxyl groups, etc. generated by voltage, current and electrolysis. From the experimental results, it was found that the distance between the electrodes was 0.5 mm, and the limit was about 3.5 V for both AC and DC. It was found that platelets were first denatured at 3.5 V or higher. However, when a high voltage pulse is applied instantaneously, a voltage higher than 3.5 V can be applied.

According to the result of the experiment conducted at Nagasaki University this time, as shown in Table 3 of the attached report, HI
When the distance between the electrodes of the V virus is 0.3 mm and a voltage of 3 V is applied after 120 hours, the number when the electric field treatment is not performed is the HIV virus number is 18,300.
In case of electric field treatment using 3V voltage
The virus number has dropped to 191. The reason why such a revolutionary effect occurs is that water or salt in the electrolyte solution is decomposed by electrolysis, and a very small amount of active oxygen or chlorine is generated on the electrode surface. It can be judged that the HIV virus has been revolutionarily inactivated by the oxidative action or the bactericidal action of chlorine.

From the above, a very small amount of active oxygen or chlorine, which does not affect the human body, is generated on the electrode surface, and a solution such as blood or plasma passes through the electrode. Then, by contacting with blood etc. on the electrode surface and using a very small amount of active oxygen or chlorine generated on the electrode surface or a carboxyl group etc. generated by other electrolysis, blood It is also possible to inactivate HIV virus, hepatitis virus, etc. present in the solution such as. Using the action of voltage or current or the toxicity of active oxygen or chlorine,
When inactivating viruses such as HIV virus, the shape of the electrodes may be any of laminated electrodes, circular electrodes, round electrodes, parallel plate electrodes, and any shape of electrodes.
However, it is only a matter of distance between electrodes, voltage, current and effective surface area of electrodes.

Voltages and currents are used to inactivate viruses such as HIV virus and hepatitis virus, and by adjusting the distance and voltage of electrodes, highly toxic active oxygen or chlorine is removed. By precisely adjusting, the amount of active oxygen or chlorine generated on the electrode surface can be adjusted to the limit by using voltage and current to generate a very small amount of active oxygen or chlorine. Therefore, a virus such as HIV virus can be inactivated by using a very small amount of active oxygen or chlorine within a range that does not affect the human body. In this case, although a direct current may be used as the current to be used, it is possible to accurately adjust the generation amount of chlorine by using alternating current because the electrode does not polarize. The poisonous chlorine can be used for a therapeutic method capable of suppressing or inactivating the poisonous HIV virus.

A spherical or porous or honeycomb-shaped or sponge-shaped filter made of an electrically conductive material, which is made of a gas permeable electrically conductive material, is polarized to polarize the negative electrode or the positive electrode to 0.
By applying a voltage from 7 V (VS.SCE) to 1.2 V (VS.SCE), the effective surface area of the breathable electrode or electrode can be widened even in low voltage in an electrolyte solution such as blood or plasma or serum components. Therefore, a large amount of electric current can be evenly flowed in the solution, and the HIV virus non-covalently bound to the cell surface in the blood is the blood or blood-separated plasma or serum. Since it is possible to uniformly and inactivate viruses such as HIV virus existing in the components, it is not possible to completely eradicate the HIV virus from the human body, but it inactivates the HIV virus in blood, By lowering the HIV virus level in the blood, the onset of AIDS can be delayed and is effective as a therapeutic means.

A conductor material made of pure gold clay, pure silver clay, pure platinum clay, gold, platinum, carbon, activated carbon, titanium, palladium, nickel or the like, and having a porous or honeycomb shape or a sponge shape. By using a breathable electrode or a filtering material or a dielectric material formed by forming a hole in the electrode, a synergistic effect of the hole formed in the breathable electrode or the filtering material or the dielectric material and the porous hole allows Since the effective surface area can be widened, 0.7V (VS.SCE) to 1.2V (V
S. SCE) even when using a low voltage, HIV virus non-covalently bound on the surface of HIV virus in the free state or infected cells infected with HIV virus, breathable electrodes or filter media or On the surface of the dielectric,
It can be electrically inactivated or removed,
When human blood is extracorporeally circulated via a polarized anode or cathode laminated electrode, the HIV virus or infected cells in the blood can be inactivated or removed.
It is effective as a therapeutic means that can delay the onset of AIDS.

When the laminated anode or cathode electrodes are polarized, the distance between the electrodes is as close as possible to 0, and even if the solution such as blood is not pressured more than necessary, it is necessary for the treatment. In addition to virus inactivation, the flow rate will be obtained, and the ammonia dissolved in the blood, which occurs as a result of acute liver failure and liver failure, will be oxidized using electrolysis or oxidation of electrodes. However, when the distance between the electrodes is 0.3 mm, which is a solution of the electrolyte and does not cause electrolysis in blood,
Even with a low voltage of about 0.5 V, the actual potential gradient is
Since it is a very high value of 16.5 V per 1 cm, which is a unit of electricity, an action of oxidizing ammonia in blood to nitric acid occurs. Furthermore, by using the laminated electrode, the effective surface area of the electrode in contact with the solution is remarkably widened, which is more effective.

Content of Reported Harmful Examination of HIV-1 Inactivation by Electric Field Treatment 1 Tsutomu Miyamoto, 1 Taro Yamamoto, 1 Shigeru Katamine, 2 Yoshiaki Nagaura, 3 Toyoichi Ueda 1 Bacteriology, 2 Nagaura Institute of Medicine, Nagasaki University, 3 Hitachi Chemical Co., Ltd. Head Office Management Planning Office

Objective of research HIV-1 is one of the viruses having extremely weak resistance, and it is known that HIV-1 is easily inactivated by physical treatment such as heating or treatment with a surfactant or various disinfectants. . However, HIV- mixed with biological materials including blood
A method for inactivating 1 without impairing the functions of cells in the material and physiologically active substances (serum proteins, etc.) has not yet been established. HIV is not such a method in the future
-1 It is considered that it will open the way to the development of new treatment and prevention methods for infectious diseases (AIDS). We focus on electric field treatment as one of the measures for that, and
The effect was examined without affecting the infectivity.

Materials and Methods 1. electrode. The two types of electrodes shown in Table 1 were used for trial purposes for this purpose. 2. HIV-1 electric field treatment. LAV / HIV-1 infected C
An HIV-1 virus stock was prepared from the EM cell culture supernatant. This virus solution was passed through the electrode at a constant speed, during which voltage of various intensities in the range of 0-3.0V (AC,
The virus solution was recovered by energizing at 60 HZ). The temperature inside the electrode was measured with time. 3. Virus recovery rate from the electrode. In order to examine the possibility of virus adsorption to the electrode, the amount of gag protein (p24) in the virus solution collected from the electrode was measured and compared with that of the virus stock stock solution. 4. HIV-1 infectious titer. The collected virus solution was adsorbed to susceptible cells MT4 for 1 hour at 37 ° C., and the unadsorbed virus was thoroughly washed and then cultured. After the culture was started, the supernatant was collected over time, and the amount of p24 therein was measured and used as an index of the infectious titer of the virus solution. 5. Measurement of intracellular invading virus. The collected virus solution was adsorbed to sensitive cells CEM for 1 hour at 37 ° C., immediately treated with 0.025% trypsin, and then the intracellular p24 amount was measured. 6. p24 measurement. Commercially available EIA kit (HIVAG-
1, Dynabot Co., Ltd.) was used. 0D492 in advance
The standard curve of p24 was obtained, and the amount of p24 was calculated based on the standard curve.

Results 1. First, a type A electrode (laminated type) was used for examination (Table 2). Passage rate of virus solution through electrode 1.1 ml / mi
n and a residence time of 10.6 min. No increase in the temperature inside the electrode (25 ° C.) was observed up to the load voltage of 1.0 V, but it increased to 28, 34, 44 ° C. at 1.5, 2.0 and 2.5 V, respectively. . Load voltage 2.0
A significant decrease in HIV-1 infectivity was observed at V or higher. Virus recovery rate from electrode and HIV-1 in recovered virus solution
Was not correlated, and the decrease in HIV-1 infectivity was considered not due to virus adsorption on the electrode. 2. Next, in order to eliminate the effect of temperature rise, the same study was conducted using a type B electrode (parallel plate type) made of surface gold plated copper having excellent thermal conductivity (Table 3). The electrode was placed in ice under the conditions of a passage speed of 0.13 ml / min in the electrode and a residence time of 17.5 min. No temperature increase was observed up to the load voltage of 3.0V. A decrease in infectious titer was already observed at 0.5 V. It was almost the same up to 2.5V, but 3.
At 0 V, a further sharp drop was recognized. At 3.0 V, some adsorption of virus on the electrode was also observed. 3. In order to know the effect of the electric field on the initial viral infection process, the amount of intracellular p24 was measured after adsorbing the virus collected from the type B electrode for 1 hour. As a control, a virus treated at 60 ° C. for 10 or 60 minutes was used. 2.4% of the untreated virus (p24) was detected from the inside of the cell, but it was dramatically reduced to 0.01% with the virus treated at 60 ° C. for 60 minutes. On the other hand, the electric field treatment virus shows a significant decrease at 1.0 V,
In other conditions, the degree of decrease was small and did not seem to correlate with the degree of infectious titer.

Discussion By passing the HIV-1 virus solution through the electric field, the infectious titer in the solution was reduced. There is a strong possibility that the electric field acts on the virus itself and causes it to lose its infectivity, not due to the adsorption of the virus by the electrodes. In the experiment using the type A electrode, the temperature inside the electrode also rises when the load voltage is increased, so the influence of the temperature should be taken into consideration, but the type B electrode inactivated the virus without increasing the temperature. Further, a detailed analysis of the results suggests that the electric field strength (V / cm) and the residence time in the electrode are important factors for virus inactivation. This time, the electric field treatment is constant H
It seems to have an IV-1 inactivating effect. Dr. Yamamoto's group at Tokyo Medical and Dental University and we used a different system (counter electrode) for HI by electric field
We have found a V-1 inactivating effect (personal communication), and we support this result. The elucidation of the mechanism of HIV-1 inactivation by electrolysis is a topic for future research, but our result suggests that the electric field may have a point of action other than the mechanism involved in virus adsorption and entry into cells. Suggestive and interesting. In addition, it is necessary to take into consideration the electrolysis effect brought about by the high electric field strength. The biggest problem with this result is that the degree of HIV-1 inactivation obtained was smaller than other inactivation treatments (such as heating) and was not perfect. In the future, we plan to set the electric field treatment conditions for the complete inactivation of HIV-1 and develop more efficient electrodes.

[0109]

[0110]

[0111]

[0112]

What was found in the above-mentioned joint research with the Department of Bacteriology, Nagasaki University School of Medicine was that the potential difference on the electrode surface was 0.5.
It was found that there was a considerable effect from V to 1.0V. This means that the applied potential is +0.2 V to +1.2 V (VS.SCE) in which the anode potential does not substantially generate oxygen.
The cathode potential is 0V to 1.0V, which does not substantially generate hydrogen.
It agrees with the theoretical value that it is effective within the range of (VS.SCE). From this, as a condition for producing the electrode, an electrode having a structure that allows the execution surface area of the anode and the cathode to be as large as possible, for example, by laminating a filtering material or a dielectric material using a sponge-shaped conductor, It is further polarized, and 0.7V to 1.2V (VS.S.S.S.
The virus, the filtering agent or the dielectric and the virus are brought into direct contact with each other by using the filtering material or the dielectric applied with the applied potential of CE) is the HIV virus, the hepatitis virus, the adult leukemia virus and the carcinogenic virus. Was found to be the most effective against.

[Brief description of drawings]

FIG. 1 is an explanatory view of the principle of the present invention, and FIG. 1 is a vertical sectional view.

FIG. 2 is a schematic structural explanatory view showing a means for inactivating a virus according to an embodiment of the present invention, and FIG. 2 is a vertical sectional view.

FIG. 3 is a schematic structural explanatory view showing a means for inactivating a virus according to an embodiment of the present invention, and FIG. 3 is a vertical sectional view.

FIG. 4 is a structural schematic explanatory view showing a means for inactivating a virus according to an embodiment of the present invention, and FIG. 4 is a vertical sectional view.

FIG. 5 is a schematic structural explanatory view showing a means for inactivating a virus according to an embodiment of the present invention, and FIG. 5 is a vertical sectional view.

FIG. 6 is a schematic structural explanatory view showing a means for inactivating a virus according to the embodiment of the present invention, and FIG. 6 is a vertical sectional view.

FIG. 7 is a schematic structural explanatory view showing a means for inactivating a virus according to an embodiment of the present invention, and FIG. 7 is a vertical sectional view.

FIG. 8 is a schematic structural explanatory view showing a means for inactivating a virus according to an embodiment of the present invention, and FIG. 8 is a vertical sectional view.

FIG. 9 is a schematic structural explanatory view showing a means for inactivating a virus according to the embodiment of the present invention, and FIG. 9 is a vertical sectional view.

FIG. 10 is a schematic structural explanatory view showing a means for inactivating a virus according to an embodiment of the present invention, and FIG. 10 is a vertical sectional view.

FIG. 11 is a structural schematic explanatory view showing a means for inactivating a virus according to the embodiment of the present invention, and FIG. 11 is a vertical sectional view.

FIG. 12 is a structural schematic explanatory view showing a means for inactivating a virus according to the embodiment of the present invention, and FIG. 12 is a vertical sectional view.

FIG. 13 is a structural schematic explanatory view showing a means for inactivating a virus according to the embodiment of the present invention, and FIG. 13 is a vertical sectional view.

FIG. 14 is a schematic structural explanatory view showing a means for inactivating a virus according to the embodiment of the present invention, and FIG. 14 is a vertical sectional view.

FIG. 15 is a schematic structural explanatory view showing a means for inactivating a virus according to an embodiment of the present invention, and FIG. 15 is a vertical sectional view.

FIG. 16 is a structural schematic explanatory view showing a means for inactivating a virus according to an embodiment of the present invention, and FIG. 16 is a vertical sectional view.

FIG. 17 is a structural schematic explanatory view showing a means for inactivating a virus according to the example of the present invention, and FIG. 17 is a vertical sectional view.

FIG. 18 is a schematic structural explanatory view showing a means for inactivating a virus according to an example of the present invention, and FIG. 18 is a three-dimensional view.

FIG. 19 is a schematic structural explanatory view showing a means for inactivating a virus according to the embodiment of the present invention, and FIG. 19 is an assembly drawing.

FIG. 20 is a schematic structural explanatory view showing a means for inactivating a virus according to the embodiment of the present invention, and FIG. 20 is an assembly drawing.

FIG. 21 is a structural schematic explanatory view showing a means for inactivating a virus according to an embodiment of the present invention, and FIG. 21 is an assembly drawing.

22 is a structural schematic explanatory view showing a means for inactivating a virus according to the embodiment of the present invention, and FIG. 22 is an assembly drawing.

FIG. 23 is a schematic structural explanatory view showing a means for inactivating a virus according to an example of the present invention, and FIG. 23 is a three-dimensional view.

FIG. 24 is a schematic structural explanatory view showing a means for inactivating a virus according to the embodiment of the present invention, and FIG. 24 is an assembly drawing.

FIG. 25 is a structural schematic explanatory view showing a means for inactivating a virus according to an embodiment of the present invention, and FIG. 25 is an assembly drawing.

FIG. 26 is a structural schematic explanatory view showing a means for inactivating a virus according to the embodiment of the present invention, and FIG. 26 is an assembly drawing.

FIG. 27 is a structural schematic explanatory view showing a means for inactivating a virus according to the embodiment of the present invention, and FIG. 27 is an assembly drawing.

28 is a schematic structural explanatory view showing a means for inactivating a virus according to the example of the present invention, and FIG. 28 is a three-dimensional view.

FIG. 29 is a structural schematic explanatory view showing a means for inactivating a virus according to the embodiment of the present invention, and FIG. 29 is an assembly drawing.

FIG. 30 is a structural schematic explanatory view showing a means for inactivating a virus according to the embodiment of the present invention, and FIG. 30 is an assembly drawing.

FIG. 31 is a structural schematic explanatory view showing means for inactivating a virus according to the embodiment of the present invention, and FIG. 31 is an assembly drawing.

32 is a structural schematic explanatory view showing a means for inactivating a virus according to the embodiment of the present invention, and FIG. 32 is an assembly drawing.

FIG. 33 is a structural schematic explanatory view showing a means for inactivating a virus according to an example of the present invention, and FIG. 33 is a three-dimensional view.

FIG. 34 is a structural schematic explanatory view showing a means for inactivating a virus according to the example of the present invention, and FIG. 34 is a top view.

FIG. 35 is a structural schematic explanatory view showing a means for inactivating a virus according to the embodiment of the present invention, and FIG. 35 is an assembly drawing.

FIG. 36 is a structural schematic explanatory view showing a means for inactivating a virus according to the example of the present invention, and FIG. 36 is a vertical sectional view.

FIG. 37 is a structural schematic explanatory view showing a means for inactivating a virus according to the example of the present invention, and FIG. 37 is a three-dimensional sectional view.

38 is a structural schematic explanatory view showing a means for inactivating a virus according to the example of the present invention, and FIG. 38 is a longitudinal sectional view.

[Fig. 39] Fig. 39 is a schematic structural explanatory view showing a means for inactivating a virus according to the example of the present invention, and Fig. 39 is a three-dimensional sectional view.

FIG. 40 is a structural schematic explanatory view showing a means for inactivating a virus according to an example of the present invention, and FIG. 40 is a three-dimensional view.

41 is a schematic structural explanatory view showing a means for inactivating a virus according to the example of the present invention, and FIG. 41 is a vertical sectional view.

42 is a schematic structural explanatory view showing a means for inactivating a virus according to the embodiment of the present invention, and FIG. 42 is an assembly drawing.

[Fig. 43] Fig. 43 is a structural schematic explanatory view showing a means for inactivating a virus according to an example of the present invention, and Fig. 43 is a vertical sectional view.

[Fig. 44] Fig. 44 is a structural schematic explanatory view showing a means for inactivating a virus according to an example of the present invention, and Fig. 44 is a three-dimensional cross-sectional view.

FIG. 45 is a structural schematic explanatory view showing a means for inactivating a virus according to an example of the present invention, and FIG. 45 is a vertical sectional view.

FIG. 46 is a schematic structural explanatory view showing a means for inactivating a virus according to the example of the present invention, and FIG. 46 is a three-dimensional sectional view.

[Fig. 47] Fig. 47 is a structural schematic explanatory view showing a means for inactivating a virus according to an example of the present invention, and Fig. 47 is a three-dimensional view.

FIG. 48 is a structural schematic explanatory view showing a means for inactivating a virus according to the example of the present invention, and FIG. 48 is a three-dimensional view.

FIG. 49 is a structural schematic view showing a means for inactivating a virus according to the example of the present invention, and FIG. 49 is a top view.

50 is a structural schematic explanatory view showing a means for inactivating a virus according to an example of the present invention, and FIG. 50 is a vertical sectional view.

51 is a schematic structural explanatory view showing a means for inactivating a virus according to the embodiment of the present invention, and FIG. 51 is an assembly drawing.

52 is a schematic structural explanatory view showing a means for inactivating a virus according to the embodiment of the present invention, and FIG. 52 is an assembly drawing.

53 is a structural schematic explanatory view showing a means for inactivating a virus according to the embodiment of the present invention, and FIG. 53 is an assembly drawing.

54 is a schematic structural explanatory view showing a means for inactivating a virus according to an example of the present invention, and FIG. 54 is a three-dimensional view.

55 is a structural schematic explanatory view showing a means for inactivating a virus according to an example of the present invention, and FIG. 55 is a three-dimensional view.

FIG. 56 is a structural schematic explanatory view showing a means for inactivating a virus according to the example of the present invention, and FIG. 56 is a top view.

FIG. 57 is a structural schematic explanatory view showing a means for inactivating a virus according to the example of the present invention, and FIG. 57 is a vertical sectional view.

58 is a structural schematic explanatory view showing a means for inactivating a virus according to the example of the present invention, and FIG. 58 is a vertical cross-sectional view.

FIG. 59 is a structural schematic explanatory view showing a means for inactivating a virus according to the example of the present invention, and FIG. 59 is a vertical sectional view.

FIG. 60 is a schematic structural explanatory view showing a means for inactivating a virus according to the example of the present invention, and FIG. 60 is a longitudinal sectional view.

FIG. 61 is a schematic structural explanatory view showing a means for inactivating a virus according to the example of the present invention, and FIG. 61 is a vertical sectional view.

62 is a structural schematic explanatory view showing a means for inactivating a virus according to the example of the present invention, and FIG. 62 is a vertical sectional view.

FIG. 63 is a structural schematic explanatory view showing a means for inactivating a virus according to the example of the present invention, and FIG. 63 is a vertical sectional view.

64 is a structural schematic explanatory view showing a means for inactivating a virus according to an example of the present invention, and FIG. 64 is a vertical sectional view.

FIG. 65 is a schematic structural explanatory view showing a means for inactivating a virus according to the example of the present invention, and FIG. 65 is a top view.

FIG. 66 is a structural schematic explanatory view showing a means for inactivating a virus according to the example of the present invention, and FIG. 66 is a top view.

67 is a schematic structural explanatory view showing a means for inactivating a virus according to the example of the present invention, and FIG. 67 is a top view.

68 is an explanatory view of the structure showing the means for inactivating a virus according to the example of the present invention, and FIG. 68 is a side view.

FIG. 69 is a schematic structural explanatory view showing a means for inactivating a virus according to the example of the present invention, and FIG. 69 is a vertical sectional view.

70 is a schematic structural explanatory view showing a means for inactivating a virus according to an example of the present invention, and FIG. 70 is a three-dimensional view.

71 is a schematic structural explanatory view showing a means for inactivating a virus according to the example of the present invention, and FIG. 71 is a vertical sectional view.

72 is a schematic structural explanatory view showing a means for inactivating a virus according to an example of the present invention, and FIG. 72 is a three-dimensional view.

[Explanation of symbols]

1. 1. A filtering device made of an insulating material such as glass or plastic (abbreviated as a filtering device) 1. 2. A breathable electrode made of gold, platinum, carbon, graphite, activated carbon, titanium, palladium, nickel, or the like which is an insoluble metal material (abbreviated as an insoluble metal electrode or electrode). Terminal 4. Crosspiece made of electrical insulating material such as plastic (abbreviated as crosspiece) 5. Filter material or dielectric made of breathable conductor (abbreviated as
5. Filter material made of breathable conductor or filter material or dielectric) 6. 7. A breathable electrode made of gold, platinum, carbon, graphite, activated carbon, titanium, palladium, nickel, aluminum, or the like (abbreviated as a breathable electrode, a power supply anode, or a power supply cathode) 7. Hole 8. O-rings made of insulators (O-rings for short) 9. Packing made of insulator (abbreviated as packing) 10. 11. Adhesive made of non-conductive material (abbreviated as adhesive) 11. One-sided adhesive tape made of non-conductive material (abbreviated as
Single-sided adhesive tape) 12. Double-sided adhesive tape made of non-conductive material (abbreviated as
Double-sided adhesive tape) 13. Coating layer coated with insulating coating material (abbreviated as insulating coating layer) 14 and 15. 16. Lids on both sides of the filtering device 1 (abbreviated as lids) 16 and 17. 17. Packing provided on both sides of the filtering device 1 (abbreviated as packing) 18. 18. Packing for maintaining airtightness of the screw 20 (abbreviated as packing) 19. A bolt for fixing the terminal 3 (abbreviated as a bolt) 20. 20. Connection part provided on the breathable electrode 6 (abbreviated as a connection part) 21. Breathable electrode that integrally forms the terminal 3 (abbreviated as
Air-permeable electrode with terminal) 22 and 23. 24. Lids provided on both side surfaces of the filtering instrument 1 (abbreviated as lids) 24. 25. Bolts whose surface is insulated or bolts made of an insulator (abbreviated as bolts) 25. 26. A nut whose surface is insulation-treated or a nut made of an insulator (abbreviated as nut) 26. Bolt 27. Electrodes provided with connection portions 28 (abbreviated as electrodes with connection portions) 28. Connection part 29. A serpentine packing for causing the solution to meander inside the electrode 27 with a connecting portion (abbreviated as serpentine packing) 30. Laminated electrode 31. Temperature control tank 32. 33. Packing with adhesive applied on both sides (abbreviated as packing with adhesive) 33. 34. A connecting portion for connecting the laminated electrode 30 and the terminal 3 (abbreviated as a connecting portion) 34. 35. Electrode connection component for making each breathable electrode 6 into a pair (abbreviated as electrode connection component) 35. 36. A connection portion provided on the electrode connection component (abbreviated as a connection portion) 36. Joint parts for branching and joining the solution (abbreviated as joint parts) 37. 38. Bolts and nuts whose surface is insulated or bolts and nuts made of an insulator (abbreviated as bolts) 38. Cylindrical electrode (abbreviated as a cylindrical electrode) 39. An electrode having a cylindrical shape or a cylindrical shape having a smaller diameter than the cylindrical electrode 38 (abbreviated as a cylindrical electrode) 40 and 41. 42.43 and 44. A lid whose surface is insulation-treated or a lid made of an insulator (abbreviated as a lid) for forming the filtering device 1. Bolts whose surfaces are insulated or bolts made of an insulator (abbreviated as bolts) 45 and 46. A fixing bolt that doubles as a terminal (abbreviated as
Terminals) 47, 48, 49, 50 and 51 Packing or O-ring made of an insulator (abbreviated as packing) 52. A sleeve made of an insulator provided with the groove 53 (abbreviated as a sleeve provided with the groove 53) 53. Groove 54. A sleeve made of an insulator having holes 7 (abbreviated as a sleeve having holes 7) 55. Sleeve made of insulator (abbreviated as sleeve) 56. Thermometer 57. Conductive spring (abbreviated as spring) 58. Junction

Claims (43)

[Claims] 1. A breathable electrode (6) inside a filtration device (1).
Alternatively, a breathable electrode (21) with a terminal or an electrode (27) with a connecting portion is provided so that the distance between the breathable electrode (6), the breathable electrode with a terminal (21) or the electrode with a connecting portion (27) is reduced. 3 mm or 2 mm or 1 mm or 0.5 mm or 0.
3 mm or 0.1 mm or 0.05 mm or less, the voltage or current to be used has a high-voltage pulse or direct current or alternating current characteristics, and is tens of thousands of volts or thousands of volts or hundreds of volts or tens of volts or 3 or 2V or 1V or 0.5V or 0.2
A solution such as blood or plasma of the human body is passed through the interior of electrodes with a high potential gradient, which is applied with a voltage of 5 V or less, and direct influence of voltage or current or chlorine generated by electrolysis is generated. HIV virus RNA or reverse transcriptase or proteolytic enzyme or H present in solution due to indirect effects
The HIV virus is inactivated by damaging or destroying the envelope or other important parts of the HIV virus on the surface of the IV virus. Alternatively, a method for inactivating a virus, which comprises oxidizing ammonia to nitric acid by electrolysis or oxidizing action of an electrode. 2. A human body blood is circulated extracorporeally, and the blood circulated extracorporeally is applied between electrodes in which a voltage within a range that does not cause electrolysis or a voltage higher than a voltage that causes electrolysis is applied. The virus according to claim (1), which is passed through to inactivate a virus such as HIV virus or hepatitis virus existing in blood or plasma component, etc. by using the action of voltage or current, and then refluxing to the human body. How to inactivate. 3. A sleeve in which another cylindrical electrode (39) having a diameter smaller than that of the cylindrical electrode (38) is inserted into the cylindrical electrode (38) and grooves (53) are provided on both side surfaces. A cylindrical electrode (39) using a sleeve (54) or sleeve (55) provided with (52) or holes (7)
Of the blood or plasma components of the human body, or limba liquid, etc., inside the filtration device (1) that is precisely fixed and that is sealed using the lid (40) and the lid (41) that are formed with internal threads from both sides. The solution is allowed to pass therethrough to be circulated extracorporeally to inactivate viruses existing in blood and the like. Alternatively, the method for inactivating a virus according to claim 1 or 2, wherein ammonia is oxidized. 4. A cooling tank (31) is provided on the outer wall portion of the cylindrical electrode (38), and cooling water is circulated inside the cooling tank (31) to cool the inside of the filtering device (1). A method for inactivating the virus according to item (1), (2) or (3). 5. A plurality of breathable electrodes (6) and a plurality of O-rings (8) provided with a connecting portion (20) for connecting with a terminal (3) inside a filtering device (1). The packing (9) is alternately laminated, and the terminal (3) is connected to the breathable electrode (6) with the bolt (19) and fixed to the connecting portion (20) provided in the breathable electrode (6). And filtering equipment (1)
A filtering instrument (1) in which the inside is completely sealed by using a lid (14) or a lid (15) provided on both side surfaces of the filter or a lid (14) provided on only one side surface. ) A solution such as blood or plasma of the human body is passed inside to inactivate viruses existing in blood and the like. Alternatively, the method for inactivating the virus according to claim 1, wherein the method comprises oxidizing ammonia. 6. A plurality of breathable electrodes (6) and a plurality of O-rings (8) or packings (9) are alternately laminated and used, or an adhesive (10) or a single-sided adhesive tape (1).
1) or double-sided adhesive tape (12) or insulating coating layer (1)
3) or a packing (32) with adhesive or the like is used or formed to form an insulating layer on the surface of the breathable electrode (6), and then a lid (22) or a lid (22) provided on both side surfaces. 23)
Using a bolt (24) or a nut (25), a solution such as blood or plasma of the human body is passed through the inside of the filtration device (1) in which the inside of the filtration device (1) is completely sealed, Inactivate the virus present in. Alternatively, the method for inactivating a virus according to claim 1, wherein the method comprises oxidizing ammonia. 7. A breathable electrode (21) with a terminal, wherein a plurality of breathable electrodes (6) are integrally formed with wiring terminals (3).
And a plurality of O-rings (8) or packings (9) are alternately laminated or used, or an adhesive (10), a single-sided adhesive tape (11), a double-sided adhesive tape (12), or an insulating coating scrap (13) ) Or using a packing (32) with an adhesive or the like, and forming an insulating layer between the breathable electrode (21) with a terminal and the breathable electrode (21) with a terminal, and then providing it on both sides Using the lid (22) or the lid (23) and the bolt (24) or the nut (25), the blood of the human body or the inside of the filtration instrument (1) in which the inside of the filtration instrument (1) is completely sealed. A virus such as blood is inactivated by passing a solution such as plasma. Or the method of oxidizing ammonia (1), (2), (3), (4),
A method for inactivating the virus according to (5) or (6). 8. A plurality of breathable electrodes (6), an intermediate between the breathable electrodes (6) and the breathable electrodes (6), an adhesive (10), a single-sided adhesive tape (11), or a double-sided adhesive tape ( 12)
Or insulation coating layer (13) or packing with adhesive (3
2) etc. or using O-rings (8) or packing (9) and bolts (37) to stack several to several tens or more breathable electrodes. A pair of replaceable cassette type laminated electrodes (3
0) into the filtration device (1) and cover the lids (1) on both sides.
4) or the lid (15) or the packing (17) provided on the lid (14) on one side is used from both sides or one side to seal both sides of the laminated electrode (30). Three
0) Maintain the airtight state inside. Furthermore, the packing (16) is used to maintain the airtightness of the terminal (3) provided on the laminated electrode (30) or the connecting portion (33) connected to the terminal (3), and the filtration device (1). A solution such as blood or plasma of the human body is passed through the inside of the body to inactivate the virus existing in the blood or the like. A method for inactivating a virus according to any one of claims (1), (2), (3), (4), (5), (6) or (7), which further comprises oxidizing ammonia. 9. O-ring (8) or packing (9) or adhesive (10) or single-sided adhesive tape (11) or double-sided adhesive tape (12) or insulating coating layer (13) or adhesive-equipped packing (32). Alternatively, when the laminated electrode (30) is formed using bolts (37) or the like, the positions of the holes (7) provided in the air-permeable electrode (6) are aligned with each other so that the holes (7) are aligned. The laminated electrode (3
0) The laminated electrode (30) in which a plurality of breathable electrodes (6) are laminated so that the solution flowing inside flows straightly and straightly inside the laminated electrode (30) without stagnation, is used as a filter device (1). ) Claims (1), (2), which are used internally
A method for inactivating the virus according to (3), (4), (5), (6), (7) or (8). 10. O-ring (8) or packing (9)
Alternatively, an adhesive (10), a single-sided adhesive tape (11), a double-sided adhesive tape (12), an insulating coating layer (13), a packing with an adhesive (32), a bolt (37), or the like is used to form a laminated electrode (30). ) Is formed, the positions of the holes (7) provided in the breathable electrode (6) are alternately provided to stack the breathable electrodes (6), and the solution flowing inside the stacked electrode (30) is A laminated electrode (30), wherein a plurality of gas permeable electrodes (6) are laminated so as to flow in a zigzag manner inside the laminated electrode (30), and the laminated electrode (30) is used inside a filtration device (1). (2), (3), (4), (5),
A method for inactivating the virus according to (6), (7), (8) or (9). 11. A filter device (1) having two connecting electrodes (27) and one packing (9) or one serpentine packing (in parallel to the longitudinal direction of the flow of a solution). Two
9) Or a plurality of electrodes with connecting parts (27) and a plurality of packings (9) or a plurality of snake-like packings (29) are alternately laminated, inside the filtration device (1), blood or plasma of the human body, etc. The solution present in (1) is passed through to inactivate the virus present in blood or the like. Further, the method of oxidizing ammonia (1), (2), (3), (4), (5), (6),
A method for inactivating the virus according to (7), (8), (9) or (10). 12. An electrode (27) having a plurality of connecting portions, which is parallel to the longitudinal direction in which a solution flows, inside the filtering device (1).
A plurality of packings (9) and a plurality of packings (9) are alternately used inside the filtration device (1), and a serpentine packing (29) for increasing the distance through which the solution inside the electrode (27) with a connecting portion flows. (1), (2), (3), (4), which is used inside the electrode (27) with a connecting portion.
A method for inactivating the virus according to (5), (6), (7), (8), (9), (10) or (11). 13. A material obtained by molding a material of a cylindrical electrode (38) or a cylindrical electrode (39) using a chemical product such as ABS resin, nylon resin or other resin, and molding the chemical product. Using a cylindrical electrode (38) or a cylindrical electrode (39), which is obtained by subjecting the surface of gold, platinum or other metal to a surface treatment using electroplating, electroless plating or the like, Claims (1), (2), (3), (4), (5), which form a filtering device (1) which is an electrode.
(6), (7), (8), (9), (10), (11)
Alternatively, a method for inactivating the virus according to (12). 14. A filtering instrument (1) is provided with a terminal (3), and the inside of the filtering instrument (1) is filled with a filtering material or a dielectric material (5) made of a breathable conductor to obtain a breathable conductive material. A filtering device (1) in which a negative or positive current is passed through a filter or dielectric (5) made of a body, and a negative or positive current is passed through a filter or dielectric (5) made of a gas permeable conductor (1) ) Inside, the water, tap water, seawater, blood, plasma, serum or other solutions are passed to kill or inactivate or oxidize bacteria, viruses and other microorganisms or ammonia, bilirubin, poisons, etc. present in the solution. Claims (1), (2), (3), (4),
(5), (6), (7), (8), (9), (10),
A method for inactivating the virus according to (11), (12) or (13). 15. A filter material (5) made of a gas permeable conductor between electrodes (2) and electrodes (2) facing each other, provided with electrodes (2) facing each other inside a filtering device (1). ), And the opposing electrode (2) and the filtering material or dielectric (5) made of a gas permeable conductor are brought into contact with each other, and the filtering material or dielectric (5) made of a gas permeable conductor is made into a negative electrode. Or, the current of the positive electrode is flowing,
Water and tap water, seawater, blood, plasma, serum or other solutions are passed through the inside of the filtration device (1) to sterilize and immobilize microorganisms such as bacteria and viruses, or ammonia, bilirubin, and toxic substances present in the solution. Claims (1), (2), (3), (4), (5), (6), which are activated or oxidized.
(7), (8), (9), (10), (11), (1
A method for inactivating the virus according to 2), (13) or (14). 16. A breathable electrode (6) inside a filtering device (1).
Are provided facing each other, and a filter material or a dielectric (5) made of a breathable conductor is filled between the facing breathable electrodes (6) and (6) to face each other. Filtration in which a negative electrode or a positive electrode current is passed through the filter material (5) made of a gas permeable conductor by bringing the gas permeable electrode (6) into contact with the filter material (5) made of a gas permeable conductor. Water or tap water, seawater, blood, plasma, serum or other solutions are passed through the inside of the device (1) to sterilize or not destroy bacteria, viruses and other microorganisms or ammonia, bilirubin, poisons, etc. present in the solution. Claims (1), (2), (3), which are activated or oxidized,
(4), (5), (6), (7), (8), (9),
(10), (11), (12), (13), (14),
Alternatively, a method for inactivating the virus according to (15). 17. A breathable electrode (6) inside a filtering device (1).
Water or tap water, seawater, blood, plasma, serum or other solution is passed through the inside of the filtration device (1) in which one or more sheets are provided, and microbe such as bacteria, virus, etc. present in the solution. Lecture items (1), (2) to sterilize, inactivate or oxidize main substances or ammonia, bilirubin, poisons, etc.
(3), (4), (5), (6), (7), (8),
(9), (10), (11), (12), (13),
A method for inactivating the virus according to (14), (15) or (16). 18. A blood, plasma component or limba liquid of a human body,
The blood that is led out of the body and circulates outside the body, and the blood that is circulated outside the body is passed between the electrodes where a voltage higher than the voltage that causes electrolysis is applied, and the toxicity of chlorine etc. generated by the electrolysis is reduced. For the purpose of softening, in the solution such as blood, plasma component or Limba solution that has passed between the electrodes,
A neutralizing agent for neutralizing toxicity such as chlorine, for example, hypo, is used to neutralize toxicity such as chlorine generated in a solution such as blood, plasma component or lymph fluid (1).
(2), (3), (4), (5), (6), (7),
(8), (9), (10), (11), (12), (1
A method for inactivating the virus according to 3), (14), (15), (16) or (17). 19. Another breathable electrode (6) provided inside a filtering device (1) so as to face the breathable electrode (6).
Are laminated with O-ring (8) or packing (9) and bolt (37), or adhesive (10) or single-sided adhesive tape (11) or double-sided adhesive tape (12)
Or insulation coating layer (13) or packing with adhesive (3
2) or the like is used to form an insulating layer between the breathable electrode (6) and the breathable electrode (6) and stack the two or more breathable electrodes (6) Bacteria and viruses existing in the solution by passing water and tap water, seawater, blood, plasma, serum or other solutions through the inside of the filtration device (1) provided with the pair of laminated electrodes (30). (1), (2), (3), which sterilizes or inactivates or oxidizes microorganisms such as or ammonia, bilirubin, poisons, etc.
(4), (5), (6), (7), (8), (9),
(10), (11), (12), (13), (14),
A method for inactivating the virus according to (15), (16), (17) or (18). 20. A breathable electrode (6) inside a filtering device (1).
Are provided facing each other, and a filter material or a dielectric (5) made of a breathable conductor is filled between the facing breathable electrodes (6) and (6) to face each other. Filter material or dielectric (5) made of breathable electrode (6) and breathable conductor
The exhaust gas emitted from an automobile engine or the like is passed through the inside of the filtering device (1) in which the negative electrode or the positive electrode current is passed through the filter material (5) made of a gas permeable conductor.
Claims (1), (2), which adsorb and remove nitrogen oxides or sulfur oxides or black smoke contained in the exhaust gas.
(3), (4), (5), (6), (7), (8),
(9), (10), (11), (12), (13),
A method for inactivating the virus according to (14), (15), (16), (17), (18) or (19). 21. The blood of the human body is led from the blood vessel or the lymph fluid to the outside of the human body to be circulated outside the body,
Blood or plasma components or lymph that are circulated outside the body,
A voltage within the range that does not cause electrolysis or a voltage that is equal to or higher than the voltage that causes electrolysis is passed between electrodes to change the ion channel on the surface of cells such as white blood cells or the change in cell membrane potential or The pump on the cell surface is stimulated by the action of voltage or current to forcibly open or close, and activates or suppresses cells to produce or release or release cytokines, interleukins or interferons. (1) for stopping the
(2), (3), (4), (5), (6), (7),
(8), (9), (10), (11), (12), (1
3), (14), (15), (16), (17), (1
8) A method for inactivating the virus according to (19) or (20). 22. Spherical particles or granules made of gold, platinum, carbon, graphite, activated carbon, titanium, palladium, nickel, or the like are used as the filtering material (5) made of a breathable conductor. (1), (2),
(3), (4), (5), (6), (7), (8),
(9), (10), (11), (12), (13),
(14), (15), (16), (17), (18),
A method for inactivating the virus according to (19), (20) or (21). 23. A filter material (5) made of a gas permeable conductor, which is made of gold, platinum, carbon, graphite, activated carbon, titanium, palladium, nickel, or the like and has a porous or honeycomb shape or a sponge shape. (1), (2), (3), (4), which uses an object made of a conductive material,
(5), (6), (7), (8), (9), (10),
(11), (12), (13), (14), (15),
(16), (17), (18), (19), (20),
A method for inactivating the virus according to (21) or (22). 24. A carbon fiber is used as a filtering material or a dielectric material (5) made of a gas permeable conductor.
(2), (3), (4), (5), (6), (7),
(8), (9), (10), (11), (12), (1
3), (14), (15), (16), (17), (1
8) A method for inactivating the virus according to (19), (20), (21), (22) or (23). 25. The surface of a filter material (5) having a spherical shape, a porous shape, a honeycomb shape or a sponge shape, which is used as a filter material or a dielectric material (5) made of breathable conductive material, is provided with a living body. Made of a conductive material that has been surface-treated with a compatible resin or an insulating resin to reduce the conductive property of the dielectric material (5) or the filtering material made of conductive material. Claims (1), (2), (3), which use a filtering material (5),
(4), (5), (6), (7), (8), (9),
(10), (11), (12), (13), (14),
(15), (16), (17), (18), (19),
(20), (21), (22), (23) or (24)
A method for inactivating the described virus. 26. One breathable electrode (6) formed by forming holes (7) in a porous material formed by molding pure gold clay, pure silver clay, pure platinum clay, etc. and firing. Alternatively, a plurality of filtering instruments (1) are used to electrically inactivate HIV virus present in blood or the like. Further, the method of oxidizing ammonia (1), (2), (3), (4),
(5), (6), (7), (8), (9), (10),
(11), (12), (13), (14), (15),
(16), (17), (18), (19), (20),
(21), (22), (23), (24) or (25)
A method for inactivating the described virus. 27. One breathable electrode (6) formed by forming a hole (7) in a porous material formed by molding pure gold clay, pure silver clay, pure platinum clay, etc. and firing. Alternatively, an HIV virus present on the surface of an infected cell that is non-covalently bound to the surface of the cell is electrically inactivated by using a plurality of filtration devices (1). , (2),
(3), (4), (5), (6), (7), (8),
(9), (10), (11), (12), (13),
(14), (15), (16), (17), (18),
(19), (20), (21), (22), (23),
A method for inactivating the virus according to (24), (25) or (26). 28. A breathable electrode (6) formed by forming holes (7) in a porous material formed by molding pure gold clay, pure silver clay, pure platinum clay, etc. (1), (2), which is used as a filter paper having various characteristics,
(3), (4), (5), (6), (7), (8),
(9), (10), (11), (12), (13),
(14), (15), (16), (17), (18),
(19), (20), (21), (22), (23),
A method for inactivating the virus according to (24), (25), (26) or (27). 29. Voltage and current are applied to leukocytes, which are the sources of human immunity to regulate the production of antibodies, and the action of an electric field regulates the actions of leukocytes, which regulates the production of antibodies. , (1), (2), (3), which artificially and freely suppress or activate leukocytes by using an electric field.
(4), (5), (6), (7), (8), (9),
(10), (11), (12), (13), (14),
(15), (16), (17), (18), (19),
(20), (21), (22), (23), (24),
A method for inactivating the virus according to (25), (26), (27) or (28). 30. A porous or honeycomb-shaped or sponge-shaped conductive material made of gold, platinum, carbon, graphite, activated carbon, titanium, palladium, nickel or aluminum etc. as the breathable electrode (6) Claims (1), (2), (3), (4) using a rest object
(5), (6), (7), (8), (9), (10),
(11), (12), (13), (14), (15),
(16), (17), (18), (19), (20),
(21), (22), (23), (24), (25),
A method for inactivating the virus according to (26), (27), (28) or (29). 31. Breathable electrode (6) or cylindrical electrode (3)
8) Or as a cylindrical electrode (39), a non-conductive synthetic resin or a metal or a non-ferrous metal is molded, and then nickel or platinum is used to perform electroplating, electroplating or vapor deposition of a conductor. Claim (1) that uses a holiday,
(2), (3), (4), (5), (6), (7),
(8), (9), (10), (11), (12), (1
3), (14), (15), (16), (17), (1
8), (19), (20), (21), (22), (2
3), (24), (25), (26), (27), (2
8) A method for inactivating the virus according to (29) or (30). 32. A filter device (1) has a double structure on the outside, a temperature control tank (31) is provided on an outer wall portion of the filter device (1), and cooling water or Claims (1), (2), (3), (4), (5), (6), in which warm water is circulated to cool the inside of the filtration device (1).
(7), (8), (9), (10), (11), (1
2), (13), (14), (15), (16), (1
7), (18), (19), (20), (21), (2
2), (23), (24), (25), (26), (2
A method for inactivating the virus according to 7), (28), (29), (30) or (31). 33. O-ring (8) or packing (9) or adhesive (10) or single-sided adhesive tape (11), comprising several to several tens or more breathable electrodes (6).
Alternatively, the laminated electrode (30) laminated by using the double-sided adhesive tape (12), the insulating coating layer (13), the packing with adhesive (32), the bolt (37), or the like, is a filtration device (1).
Claims (1), (2), (3), which are used internally
(4), (5), (6), (7), (8), (9),
(10), (11), (12), (13), (14),
(15), (16), (17), (18), (19),
(20), (21), (22), (23), (24),
(25), (26), (27), (28), (29),
A method for inactivating the virus according to (30), (31) or (32). 34. A state in which human blood is extracorporeally circulated, voltage and current are applied to the blood circulated extracorporeally, and the production of antibodies is reduced by the action of an electric field to reduce human immunity. At (1), (2), the transplantation of an organ of another person or an animal is performed, the rejection reaction is reduced by the action of an electric field, and the organ of the other person or an animal is smoothly transplanted and fixed.
(3), (4), (5), (6), (7), (8),
(9), (10), (11), (12), (13),
(14), (15), (16), (17), (18),
(19), (20), (21), (22), (23),
(24), (25), (26), (27), (28),
(29), (30), (31), (32), or (3
3) A method for inactivating the virus described. 35. The voltage or current used is a high voltage pulse, a low voltage pulse, an arbitrary pulse waveform, a biboler pulse, an alternating current or a direct current or other alternating current superposed with a direct current,
Claims (1), (2), (3), (4), (5), (6), which use a voltage or current having the property of direct current.
(7), (8), (9), (10), (11), (1
2), (13), (14), (15), (16), (1
7), (18), (19), (20), (21), (2
2), (23), (24), (25), (26), (2
7), (29), (30), (31), (32), (3
A method for inactivating the virus according to 3) or (34). 36. A plate-shaped or expanded mesh-shaped perforated blade-shaped porous structure in which a plurality of porous or spherical or sponge-shaped filtering materials or dielectrics (5) are placed in a DC electric field and installed at both ends. A direct current voltage or an alternating current voltage is applied between the power feeding anode (6) and the power feeding cathode (6), each of which is composed of a plate, and the filtering material or the dielectric (5) is applied.
Cells, such as red blood cells and white blood cells, inside a filtration device (1) containing three-dimensional electrodes that polarize the filter material or the dielectric material (5) to form an anode and a cathode at one end and the other end, respectively. The whole blood containing the portion or the plasma component obtained by separating the whole blood is allowed to pass through to be released into the HIV virus, the hepatitis virus, the adult leukemia virus or the solution which is non-covalently bound on the cell surface. (1) which inactivates HIV virus, hepatitis virus, adult leukemia virus or other virus in a free state,
(2), (3), (4), (5), (6), (7),
(8), (9), (10), (11), (12), (1
3), (14), (15), (16), (17), (1
8), (19), (20), (21), (22), (2
3), (24), (25), (26), (27), (2
8), (29), (30), (31), (32), (3
3) A method for inactivating the virus according to (34) or (35). 37. A hole diameter formed in a porous or sponge-shaped filter material or a dielectric material (5) has a hole diameter through which whole blood can pass, for example, a hole diameter of 100 to 300 microns. Using a filter material (1) provided with a filter material or a dielectric (5) having a hole diameter up to the maximum, the blood of the human body is taken out and circulated, and the blood circulated outside the body is circulated. An HIV virus or blood that is passed through the inside of a filtration device (1) provided with a filtering material or a dielectric (5) and is non-covalently bonded on the surface of cells without affecting the cell parts in the blood. After inactivating the free HIV virus or other viruses present in it,
Claims (1), (2), (3), which are returned to the human body again,
(4), (5), (6), (7), (8), (9),
(10), (11), (12), (13), (14),
(15), (16), (17), (18), (19),
(20), (21), (22), (23), (24),
(25), (26), (27), (28), (29),
(30), (31), (32), (33), (34),
A method for inactivating the virus according to (35) or (36). 38. The blood of the whole blood circulated extracorporeally or the plasma component obtained by separating the blood is passed through from the direction of forming the power supply cathode (6) provided inside the filtration device (1). , After inactivating the HIV virus or hepatitis virus or adult leukemia virus that is non-covalently bound on the surface of the cell, or the free HIV virus or hepatitis virus or adult leukemia virus or other virus present in the blood, Blood (1), (2), (3), (4), (5), (6), which recirculates blood to the human body again.
(7), (8), (9), (10), (11), (1
2), (13), (14), (15), (16), (1
7), (18), (19), (20), (21), (2
2), (23), (24), (25), (26), (2
7), (28), (29), (30), (31), (3
2) A method for inactivating the virus according to (33), (34), (35), (36) or (37). 39. A filter material having a porous or sponge-shaped filter material or a dielectric material (5) on which an permeable metal electrode (2) made of an insoluble metal material is attached or adhered to the anode part, or Filtration device (1) provided with a dielectric (5)
HIV or hepatitis virus or adult leukemia virus that is non-covalently bound to the surface of cells by passing whole blood or plasma components obtained by separating blood, or HIV in a free state present in blood After inactivating the virus, hepatitis virus, adult leukemia virus, or other virus, whole blood is recirculated to the human body again (1), (2), (3), (4), (5). ,
(6), (7), (8), (9), (10), (1
1), (12), (13), (14), (15), (1
6), (17), (18), (19), (20), (2
1), (22), (23), (24), (25), (2
6), (27), (28), (29), (30), (3
1), (32), (33), (34), (35), (3
6) A method for inactivating the virus according to (37) or (38). 40. The applied potential has an anode potential of +0.2 V to +1.2 V (VS.SC) without substantial oxygen generation.
E), the cathode potential is substantially 0 V to − without hydrogen generation.
Blood of whole blood or plasma component obtained by separating blood in a filtering device (1) provided with a filtering material or a dielectric (5) applying a voltage within a range of 1.0 V (VS.SCE). HIV virus or hepatitis virus or adult leukemia virus that is non-covalently bound to the surface of cells without affecting the cell part in blood, or HIV virus or hepatitis in free state present in blood After inactivating the virus or adult leukemia virus or other viruses, whole blood is recirculated to the human body again (1), (2), (3), (4), (5), (6). ),
(7), (8), (9), (10), (11), (1
2), (13), (14), (15), (16), (1
7), (18), (19), (20), (21), (2
2), (23), (24), (25), (26), (2
7), (28), (29), (30), (31), (3
2), (33), (34), (35), (36), (3
A method for inactivating the virus according to 7), (38) or (39). 41. A crosspiece made of a porous or breathable electrically insulating material or the like between the power feeding anode (6) and a plurality of filter media or dielectrics (5) and the power feeding cathode (6). 4) is used as an electrically insulating spacer to prevent short circuits, and whole blood or plasma components from which blood has been separated is placed inside a filtration device (1) in which the distance between multiple electrodes is minimized. HIV virus or hepatitis virus or adult leukemia virus which is non-covalently bound to the surface of cells without passing through and affecting the cell part in blood, or HIV virus or hepatitis virus in free state present in blood Alternatively, after inactivating the adult leukemia virus or other viruses, whole blood is returned to the human body again (1), (2), (3), (4), (5), (6). ),
(7), (8), (9), (10), (11), (1
2), (13), (14), (15), (16), (1
7), (18), (19), (20), (21), (2
2), (23), (24), (25), (26), (2
7), (28), (29), (30), (31), (3
2), (33), (34), (35), (36), (3
A method for inactivating the virus according to 7), (38), (39) or (40). 42. A power supply anode (6) for giving a positive DC current
As the material of the power supply anode, a carbon material (for example, activated carbon, charcoal, coke), a graphite material (for example, carbon fiber, carbon cloth, carbon composite material), or a material supporting platinum, vanadium or nickel is used. In contrast to (6), the power feeding cathode (6) for applying a negative DC voltage is formed by using, for example, platinum, graphite, a carbon material, or a metal material coated with a platinum group metal. ) And a plurality of filter media or a filter (1) provided with a plurality of dielectrics (5) are passed through the blood of whole blood or the plasma component obtained by separating the blood without affecting the cell portion in the blood. HIV non-covalently bound to the surface of cells
A virus or hepatitis virus, an adult leukemia virus, or a free HIV virus or hepatitis virus, an adult leukemia virus, or another virus present in blood, is inactivated, and then whole blood is recirculated to the human body. 1), (2), (3), (4), (5),
(6), (7), (8), (9), (10), (1
1), (12), (13), (14), (15), (1
6), (17), (18), (19), (20), (2
1), (22), (23), (24), (25), (2
6), (27), (28), (29), (30), (3
1), (32), (33), (34), (35), (3
6) A method for inactivating the virus according to (37), (38), (39), (40) or (41). 43. In order to produce a safe blood product, the blood-separated plasma component is passed between electrodes to which a voltage is applied, and the HIV virus, hepatitis virus, present in the plasma component, Claims (1), (2), (3), (4), which are intended to produce a safe blood product by inactivating carcinogenic viruses and other viruses.
(5), (6), (7), (8), (9), (10),
(11), (12), (13), (14), (15),
(16), (17), (18), (19), (20),
(21), (22), (23), (24), (25),
(26), (27), (28), (29), (30),
(31), (32), (33), (34), (35),
(36), (37), (38), (39), (40),
A method for inactivating the virus according to (41) or (42).