JPH0856657A - Deactivation of virus - Google Patents

Abstract

PURPOSE: To kill, deactivate or oxidize the microbes such as bacteria or virus, or ammonia, bilirubin, toxics found in supply water, sewage, blood, plasma components, seawater and other solutions. CONSTITUTION: Human blood is circulated out of the body, and passed through the space between electrodes or magnetic fields subjected, respectively, to the application of a voltage enough to cause or short of causing the electrolysis of the blood or to the application of a magnetic force, to deactivate the virus such as HIV virus or hepatitis virus found in the blood and plasma components by making use of the action of the voltage and electric current or magnetic field, and the resultant blood is recirculated to the human body.

Description

Detailed Description of the Invention

[Field of Industrial Application] The present invention sterilizes and inactivates bacteria, viruses and other microorganisms or ammonia, bilirubin, toxic substances and the like present in water and sewage water, blood, plasma, serum components and other solutions. Or, it relates to the technology required for oxidation. The present invention relates to a technique necessary for adsorbing and recovering nitrogen oxides or sulfur oxides, black smoke, or the like discharged from an automobile engine or the like.

PRIOR ART Conventionally, as a method for sterilizing and inactivating viruses, drugs and vaccines have been used to sterilize and inactivate viruses.
At present, no effective treatment method has been established for viruses such as V virus, hepatitis virus and carcinogenic virus.

[Problems to be Solved by the Invention] Sterilization and inactivation or oxidation of bacteria, viruses and other microorganisms or ammonia, bilirubin, toxic substances and the like present in water and sewerage water, blood, plasma, serum components and other solutions. The purpose is to let. Voltages, currents or magnets are used to activate cells, with the purpose of stimulating the cells, activating the cells and causing the cells to produce and release the cytokines interleukin and interferon. To do.

[Means for Solving the Problem] FIG. 1 is an explanatory view of the principle of the present invention. FIG. 1 is a longitudinal sectional view, in which 1 is a filtering instrument, 2 is an electrode, 3 is a terminal, Reference numeral 4 is a crosspiece, 5 is a filter material made of a breathable conductor, and 7 is a hole.

[Operation] HIV in a free state existing in blood is used by using a voltage and current or a magnet within a range that does not destroy important cells in blood such as red blood cells, white blood cells and platelets. Blood that inactivates or suppresses HIV virus that is non-covalently bound to the surface of virus or cells, and that is circulated extracorporeally outside the human body, is separated into blood cells such as red blood cells, white blood cells, and platelets using a centrifuge. After separating the cells into plasma and serum components and moving the HIV virus into the plasma and serum components, the breathable electrode 6 is placed in the plasma and serum components using one or more or a magnet. The transferred HIV virus is electrically inactivated.

[0006] As a means for uniformly applying an electric current in a solution, spherical particles or granules or porous or honeycomb shapes made of a conductor such as gold, platinum, carbon, graphite, activated carbon, titanium, palladium or nickel Alternatively, when a solution of an electrolyte such as blood or seawater is passed through the inside of the filtering instrument 1 in which a sponge-shaped object or carbon fiber is used as the filtering material 5, a low voltage is obtained without increasing the temperature of the solution. However, since a large amount of current of the negative electrode or positive electrode can be evenly flowed in a solution such as blood or seawater, bacteria, viruses and other microorganisms present in the solution, or ammonia, bilirubin, toxic substances, etc. are uniformly sterilized. And can be inactivated or oxidized.

When an electric current is passed through a solution of an electrolyte such as blood or plasma separated from blood, a serum component or seawater, the current is not passed through the electrolyte solution without using the filter medium 5 made of a gas permeable conductor. When a current is passed, an extremely thin passage through which an electric current flows is formed in the solution, and the electric current has a property of flowing only in a desired place in the solution.
There is a problem that the current cannot be evenly flowed in the electrolyte solution unless it is used.

[0008] Currently, as a means for adsorbing and removing ammonia, bilirubin, poisons and the like in blood, a product with a trade name of hemofilter or hemocolumn is used for therapy. This hemofilter or hemocolumn was made of activated carbon in which the activated carbon was made into a spherical shape of about 0.5 mm, and the surface of particles made of activated carbon was coated using a biocompatible heparin coat or the like compatible with the living body. Particles used as the filter medium 5 to remove ammonia, bilirubin, poisons, etc. in blood from the activated carbon 5
It is adsorbed on and removed.

By using the present invention, the terminals 3 are attached to both ends of the filtering instrument 1 filled with the spherical filtering material 5 made of activated carbon having a trade name of hemofilter or hemocolumn as described above. As shown in FIGS. 1 and 2, the electrode 2 or the breathable electrode 6 is provided so as to come into contact with the spherical filter medium 5 made of activated carbon that is provided inside the filter device 1. Provided, spherical filter material 5 made of activated carbon
In addition, the current of the negative electrode or the positive electrode within the range that does not affect the blood and the living body is charged, so that the surface of the activated carbon is charged and an electrical oxidation action occurs, so that ammonia in the blood is removed. The action of oxidizing to nitric acid occurs.

When free HIV virus present in blood or infectious cells innumerably non-covalently bound to HIV virus are passed through the inside of the filtration device 1 shown in FIGS. 1 and 2. When a direct-current or alternating-current electric field is applied to the spherical filter medium 5 made of activated carbon, which is filled in the filter instrument 1, the filter medium in which the infected cells are charged is applied inside the filter instrument 1. 5, the HIV virus in a free state or the HIV virus non-covalently bound on the surface of the cell is electrically affected by repeated contact with the RNA, reverse transcriptase or proteolysis of the HIV virus. The enzyme or envelope on the surface of the HIV virus or other important part of the HIV virus is electrically destroyed.

The surface of the spherical or porous or honeycomb-shaped or sponge-shaped filter material 5 used as the filter material 5 made of a breathable conductor is compatible with a living body such as a heparin coat. Use a filtering material 5 made of a conductor, which is surface-treated using a compatible resin or an insulating resin to reduce the conductive property of the filtering material 5 made of a conductor. By doing so, there is a characteristic that the charge capacity of the filter medium 5 made of a conductor is increased.

The blood of the human body is led out of the human body,
The blood circulating outside the body is molded from pure gold clay, pure silver clay, pure platinum clay, etc., manufactured by Mitsubishi Materials Corp.
Using a breathable electrode 6 formed by forming holes 7 in a material made of pure gold, pure silver, pure platinum clay or the like having a porous property similar to unglazed, in which numerous holes of about micron size are present, Due to the synergistic effect of the hole 7 provided in the breathable electrode 6 and the hole having a porous property similar to unglazed, the effective surface area of the breathable electrode 6 is significantly increased.

Regarding the HIV virus, electric fields and currents,
On November 26th, 1993 and February 16th, 1994, Professor Nagamoto of Nagasaki University, Professor Miyamoto, Associate Professor Katamine, graduate student Taro Yamamoto, Hitachi Chemical Co., Ltd. and the applicant of the present invention, Nagaura. The experimental results of the joint research conducted with Yoshiaki revealed that the HIV or RNA or reverse transcriptase or proteolysis was caused by the influence of electric field and electric current, electrolysis between electrodes, or other influences. Damage or destruction of the enzyme or the envelope on the surface of the HIV virus or other important parts of the HIV virus may result in inhibition of the HIV virus's ability to grow and inactivation.
It became clear from the following experimental results. In the case of 1 V, the HIV virus could be inactivated to about 1/10 or less when the distance between the electrodes was 0.3 mm and the energization time was 17 minutes. In the case of 2 V, the distance between the electrodes was 1.0 mm, and the inactivation could be reduced to about ⅕ or less in 10 minutes.

From the above experimental results, it can be said that there is no correlation between the inactivation of HIV virus and the electric field strength, but there is a relation between electric field strength or other factors and time. Only that turned out at least. The report on HIV virus inactivation and electric field was reported in the report of the Ministry of Health, Labor and Welfare AIDS treatment method and prevention group in 1993.
Although the report will be published by the Ministry of Health and Welfare, please refer to the outline of the report attached to the application documents.

The blood of the human body is circulated extracorporeally, and the blood circulated outside the body is circulated in FIGS. 3, 4, 5, 6, 8 and 1.
1, FIG. 14, FIG. 15, FIG. 16, FIG. 17, FIG.
9, FIG. 20, FIG. 21, FIG. 24, FIG. 33, FIG. 40, FIG.
As shown in FIG. 7, FIG. 48, FIG. 54, etc., by passing through the inside of the filtration device 1, the ammonia present in the blood is electrolyzed or the oxidation reaction of the electrodes is used, and a very low voltage is used. Then, ammonia is added to No, No ₂ , and No _3.
According to a joint study with Dr. Yasuhiro Yamada of the Kyushu Industrial Technology Research Institute, ammonia is finally transferred to nitric acid when it is oxidized by using the oxidation action of active oxygen that gradually occurs on the surface of the electrode. discovered. At the same time, it was discovered that when carbon is used as an electrode material, a carboxyl group is formed by the action of active oxygen generated on the electrode surface.

By utilizing the above-mentioned discovery of the oxidative action of ammonia, the treatment of acute liver failure or liver failure, which is now considered as an incurable disease, which occurs when ammonia cannot be decomposed in the liver As a method, ammonia dissolved in blood is oxidized using the oxidizing action of the electrode, and the toxicity is 1/10 to 1/2 that of ammonia.
It can be changed to nitric acid of 0 or less and excreted from the kidney of the human body, or can be converted to nitric acid that can be excreted from the human body by artificial dialysis using artificial kidney storage.
An artificial liver organ functioning as an artificial liver can be produced, and it can be used as a method for treating acute liver failure or liver failure.

A large amount of electric current is applied to seawater to kill and inactivate microorganisms such as bacteria and viruses in seawater. When seafood is cultivated and cultivated in seawater, no fish disease occurs. The state can suppress fish disease.

[Embodiment] An embodiment of the present invention will be described below with reference to FIG. FIG. 2 is a schematic structural explanatory view showing a virus inactivating means of the embodiment of the present invention. FIG. 2 is a longitudinal sectional view, in which 1 is a filtering instrument, 3 is a terminal, and 5 is a breathable conductive material. A filter material made of a body, 6 is a breathable electrode, and 7 is a hole.

As shown in FIG. 2, the inside of the filtering device 1 is filled with a filter material 5 made of a conductor and made of a conductor and having a spherical shape with a diameter of about several millimeters. Then, the negative electrode or the positive electrode current is caused to flow through the spherical filtering material 5 made of a conductive material by bringing it into contact with the breathable electrodes 6 provided above and below, and the filtering material 5 made of a breathable conductive material. In addition, the blood of the human body is drawn out of the body into the inside of the filtering device 1 in which the current of the negative electrode or the positive electrode is flowing, and the blood of the whole blood or the plasma or serum component obtained by separating the blood is filtered in the same manner as the artificial dialysis procedure. Existence in the blood, plasma or serum component of whole blood by circulating the blood inside the device 1 and applying voltage and current to the blood or plasma or serum component of the whole blood circulating in the outside of the device 1 Free HIV virus and non-covalently bound on the surface of the cell The HIV virus and were electrically repel shows a state in which the or sterilization and inactivate adsorbed was no or electrically to pass through the inside of the filter material 5 made of breathable conductor.

The use of the above methods and means to oxidize acute liver failure and the ammonia that dissolves in the blood resulting from liver failure using electrolysis or the oxidative action of the electrodes, Ammonia in blood can be oxidized to nitric acid.

An embodiment of the present invention will be described below with reference to FIG. FIG. 3 is a schematic structural explanatory view showing a means for inactivating a virus according to the embodiment of the present invention, and FIG. 3 is a vertical sectional view showing
1 is a filtering instrument, 3 is a terminal, 6 is a breathable electrode, 7 is a hole, 8 is an o-ring, and 9 is a packing.

FIG. 3 shows that the blood of the human body is drawn outside the body of the human body and the blood of the whole blood which is circulated outside the human body, or the blood is subjected to a centrifugal separator to obtain red blood cells, white blood cells, platelets, etc. Cells and plasma and serum components,
After moving the HIV virus in the free state, which was present in the blood, into the plasma and serum components, the plasma and serum components containing the HIV virus are filtered, and the filtering device 1 is provided with the breathable electrode 6. The HIV virus, which has an electric property, is passed through the inside of the breathable electrode 6, electrically inactivated, and contained in the blood or plasma of whole blood and serum components. Blood of whole blood after inactivating or removing HIV virus in the human body is returned to the human body, or plasma and serum components are combined with cells such as red blood cells, white blood cells and platelets separated by a centrifuge, It shows the state of reflux.

An embodiment of the present invention will be described below with reference to FIG. FIG. 4 is a schematic structural explanatory view showing a means for inactivating a virus according to the embodiment of the present invention, and FIG. 4 is a vertical cross-sectional view.
1 is a filtering instrument, 3 is a terminal, 6 is a breathable electrode, 7 is a hole, 8 is an o-ring, and 9 is a packing.

The air-permeable electrode 6 is shown in FIG.
Infected cells infected with HIV virus and HIV virus using the filtration device 1 provided with four or more breathable electrodes in the same manner as described in FIG. After inactivating or removing the HIV virus non-covalently bound to the surface of, the HIV virus in the blood is inactivated or removed, and the cleaned blood or plasma component of whole blood is returned to the human body. It shows the state.

An embodiment of the present invention will be described below with reference to FIG. FIG. 5 is a schematic structural explanatory view showing a means for inactivating a virus according to the embodiment of the present invention, and FIG.
1 is a filtering instrument, 3 is a terminal, 6 is a breathable electrode, and 7 is a hole.

The air-permeable electrode 6 is shown in FIG.
3 and 4 by using the filtering device 1 having one sheet
HIV virus and H
It shows a state in which infected cells infected with IV virus are inactivated or removed.

An embodiment of the present invention will be described below with reference to FIG. FIG. 6 is a structural schematic explanatory view showing a means for inactivating a virus according to the embodiment of the present invention, and FIG. 6 is a vertical sectional view showing
1 is a filtering device, 3 is a terminal, 6 is a breathable electrode, 7 is a hole, and 9 is a packing.

FIG. 6 shows that the same as the breathable electrode 6 is provided inside the filtering instrument 1 so as to face the breathable electrode 6 and in the middle of another breathable electrode 6. Shape hole 7
The inside of the filtering device 1 in which the packing 9 made of an insulating material, which forms the above-mentioned structure, is sandwiched, is used in the same manner as described in FIG.
After inactivating or removing the HIV virus that is non-covalently bound to the surface of infected cells infected with the virus, the HIV virus in the blood is inactivated or removed, and clean blood is returned to the human body. It shows the state.

In the case of the electrode structure shown in FIG. 6, the distance between the electrodes can be made extremely small, so that the potential gradient can be easily increased, which affects the living body. Even if the potential difference is 1 V, which is a voltage that does not apply a voltage, if the distance between the electrodes is 0.1 mm, the unit of the voltage applied to the object is 1 cm, so 1 cm / 0.1 mm = 100 times Therefore, even if the potential difference is 1 V, the distance between the electrodes is 0.1 m.
In the case of m, the voltage of 100V will be affected.

Shown in FIG. 7 is an enlarged view of the electrode structure shown in FIG. 6, in which the thickness of the hole 7 is larger than the thickness of the packing 9 which sandwiches the breathable electrode 6 in a sandwich shape. Since the major axis is large, the lines of electric force are generated in the direction of the arrow.
Since the line of electric force is generated in the direction of the arrow, the influence of the line of electric force is small in the central portion of the hole 7. Therefore, it is effective to reduce the major axis of the hole 7 formed in the breathable electrode 6 and the packing 9. Be correct.

An embodiment of the present invention will be described below with reference to FIG. FIG. 8 is a schematic structural explanatory view showing a means for inactivating a virus according to the embodiment of the present invention, and FIG. 8 is a vertical cross-sectional view.
1 is a filtering device, 3 is a terminal, 6 is a breathable electrode, 7 is a hole, 10 is an adhesive, 11 is a single-sided adhesive tape, 12
Is a double-sided adhesive tape, 13 is an insulating coating layer, 30 is a laminated electrode, and 32 is a packing with adhesive.

What is shown in FIG. 8 is different from that shown in FIG. 6 in that an adhesive 10 or a single-sided adhesive tape is used to bond two or more breathable electrodes 6 to each other. 11 or the double-sided adhesive tape 12, the insulating coating layer 13, or the packing 32 with an adhesive is used or formed to insulate the air-permeable electrode 6 and the air-permeable electrode 6 from each other, and The distance from the breathable electrode 6 can be made as small as possible, which is different from that of the laminated electrode 30 which has the highest potential gradient and has air permeability.

FIG. 9 is an enlarged view of the electrode structure shown in FIG. 8, in which the breathable electrode 6 and the breathable electrode 6 are bonded with an adhesive 10 or a single-sided adhesive tape 11 or double-sided adhesive. By using the tape 12, the insulating coating layer 13, the packing 32 with an adhesive, or the like, by stacking two or more breathable electrodes 6, the distance between the breathable electrodes 6 can be minimized. As in the case shown in FIG. 7, since the distance between the breathable electrodes 6 is smaller than the major axis of the hole 7, the line of electric force is the direction of the arrow. It shows the state that occurs in.

From the above, for example, when the distance between the breathable electrodes 6 is 1 mm, the voltage that does not affect the normal cells of the living body is around 2V. If the distance between the electrodes is 1 mm and a voltage of 2 V is applied to the HIV virus under exactly the same conditions, 90% or more of the HIV virus can be inactivated. However, when the distance between the electrodes is 10 mm and the voltage is 2 V, the HIV virus can hardly be inactivated.
For the purpose of completely inactivating the HIV virus, if a voltage of 2 V or more is applied with the distance between the electrodes being 10 mm, normal cells of the living body will be destroyed. The solution is to reduce the distance between the electrodes as much as possible to increase the potential gradient. For example, if the distance between the electrodes is 0.1 mm, then 2 V when the distance between the electrodes is 10 mm, the distance between the electrodes is 1/100.
2V when the distance between the electrodes is 0.1 mm has the same potential gradient as 200 V when the distance between the electrodes is 10 mm.

For example, if the distance between the electrodes is set to 0.1 mm, by applying a voltage of about 1 V, which does not affect normal cells of the living body and does not cause electrolysis, It is the same as the potential gradient of 100 V when the distance between the electrodes is 10 mm. The distance between the electrodes is extremely small, and the inside of the electrode structure with high potential gradient is
Since the actual voltage is 1 V even when whole blood is passed, it does not destroy living cells such as red blood cells, white blood cells, and platelets, and does not cause electrolysis.
Free HIV virus present in blood or HIV virus non-covalently bound on the surface of infected cells,
Only the HIV virus can be selectively inactivated by using the influence of voltage and current. This has been proved by joint research with a research team in the Department of Bacteriology, Nagasaki University. For some reason, it has been deleted due to space limitations, but a rough report is attached, so please refer to it.

An embodiment of the present invention will be described below with reference to FIG. FIG. 10 is a structural schematic explanatory view showing a means for inactivating a virus according to the embodiment of the present invention, and FIG. 10 is a longitudinal sectional view,
In the figure, 1 is a filtering instrument, 2 is an electrode, 3 is a terminal, 4 is a crosspiece, 5 is a filtering material made of a gas permeable conductor, and 7 is a hole.

FIG. 10 shows the filtering device 1
The two electrodes facing each other, which are provided inside the filtration device 1, are filled with a filter material 5 made of a conductor and having a spherical shape with a diameter of about several millimeters, and made of a conductor. 2 is brought into contact with 2 to pass an electric current through the spherical filter material 5 made of an electric conductor, and an electric current is passed through the filter material 5 made of a gas permeable electric conductor. It shows a state in which the exhaust gas discharged by the above, etc. is passed and nitrogen oxides or sulfur oxides or black smoke contained in the exhaust gas are adsorbed and removed.

An embodiment of the present invention will be described below with reference to FIG. FIG. 11 is a schematic structural explanatory view showing a virus inactivating means of the embodiment of the present invention. FIGS. 11, 12 and 13 are longitudinal sectional views, in which 1 is a filtering instrument and 3 is a terminal. 4 is a crosspiece, 5 is a filter material made of breathable conductor, 6
Is a breathable electrode, and 7 is a hole.

FIG. 11 shows the filtering device 1
Inside, the two breathable electrodes 6 are provided facing each other, and inside the facing breathable electrodes 6 are made of a conductor, and are made of a conductor having a spherical shape with a diameter of about several millimeters. Filter material 5
And contacting the electrodes 6 facing each other, an electric current is caused to flow through the spherical filter material 5 made of a conductive material, and the air permeable electrode 6 and the filter material 5 made of a gas permeable conductive material, The blood of the human body is led to the outside of the human body from the direction of the arrow in the inside of the filtering instrument 1 in which an electric current is passed, and the blood of the whole blood circulated extracorporeally or the plasma component obtained by separating the blood is passed therethrough, It shows a state in which the HIV virus contained in the blood or plasma component of blood is inactivated by the action of an electric field or an electric current and then returned to the human body.

When blood is passed through the inside of the filtration device 1 as shown in FIG. 11, it is non-covalently bound to the surface of HIV virus or cells contained in the blood in a free state. The existing HIV virus is inactivated or adsorbed by the influence of the potential gradient inside the filtration device 1.

As shown in FIGS. 12 and 13, the potential gradient of the filter medium 5 made of a gas permeable conductor contained in the filter device 1 is as shown in FIG. The voltage is 0 in the central part of the inside of 1 and the potential gradient is +1, +2, + from the part where the voltage of the central part is 0 toward both ends of the electrode 6 having the terminals 3 at both ends.
3, +4, +5 and the closer to the positive electrode breathable electrode 6,
The voltage becomes high. Also, the negative electrode is directed toward the negative electrode,
The closer to -1, -2, -3, -4, -5 and the negative air-permeable electrode 6, the higher the voltage.

An embodiment of the present invention will be described below with reference to FIG. FIG. 14 is a schematic structural explanatory view showing means for inactivating the virus of the example of the present embodiment, and FIG. 14 is a vertical sectional view,
In the figure, 1 is a filtering instrument, 2 is an electrode, 3 is a terminal, 4 is a crosspiece, 5 is a filtering material made of a gas permeable conductor, and 7 is a hole.

FIG. 14 shows the filtering device 1
The breathable electrode 6 is provided inside, the two electrodes 2 are provided so as to face the breathable electrode 6, and the two electrodes 2 face each other.
The inside is filled with a filter material 5 made of a breathable conductor, which is made of a conductor and has a spherical shape with a diameter of about several millimeters.
Inside the filter device 1 in which the electrode 2 and the filter material 5 made of a gas permeable conductor are in contact with each other, the blood of the human body is supplied from the direction of the arrow.
The HIV virus contained in the blood or plasma component of whole blood, which is introduced into the body of the human body and is passed through the blood or plasma component of the whole blood that is circulating outside the body, passes through the action of electric field or electric current. It shows a state in which blood is returned to the human body after being inactivated by.

An embodiment of the present invention will be described below with reference to FIG. FIG. 15 is a structural schematic explanatory view showing the means for inactivating the virus of the example of the present embodiment, and FIG. 15 is a vertical sectional view,
In the figure, 1 is a filtering instrument, 2 is an electrode, 3 is a terminal, 4 is a crosspiece, 5 is a filtering material made of a gas permeable conductor, and 7 is a hole.

FIG. 15 is different from the case shown in FIG. 14 in the following points. Two breathable electrodes 6 are provided to face each other inside and above the filtering instrument 1, and inside the facing breathable electrodes 6, a spherical shape made of a conductor and having a diameter of about several millimeters is provided. The filter material 5 made of a conductive material is filled and brought into contact with the two air-permeable electrodes 6 provided on the upper and lower sides, and an electric current is applied to the spherical filter material 5 made of a conductive material. Filtration device 1 in which an electric current is passed through a filtering material 5 made of a permeable electrode 6 and a permeable conductor.
Inside, the blood of the human body is drawn out of the body of the human body in the direction of the arrow, and the blood of the whole blood or the plasma component obtained by separating the blood from the extracorporeal circulation is passed through and contained in the blood or the plasma component of the whole blood. The infecting HIV virus is inactivated by the action of an electric field or an electric current, and then blood is returned to the human body.

An embodiment of the present invention will be described below with reference to FIG. FIG. 16 is a structural schematic explanatory view showing the means for inactivating the virus of the example of the present embodiment, and FIG. 16 is a vertical sectional view,
In the figure, 1 is a filtering instrument, 2 is an electrode, 3 is a terminal, 4 is a crosspiece, 5 is a filtering material made of a gas permeable conductor, and 7 is a hole.

FIG. 16 shows the filtering device 1
Two electrodes 2 are provided facing each other inside, and inside the facing electrodes 2 are made of a conductive material, and are made of a breathable conductive material having a spherical shape with a diameter of about several millimeters. The filtering instrument 1 which is filled with the filtering material 5 and is in contact with the two electrodes 2 facing each other is used as the first electrode and the second electrode, or the filtering instrument 1 or the first electrode and the second electrode are A pair of two electrodes, which are paired in series, are provided in a laminated electrode. Inside the filtering device 1, blood of the human body is guided to the outside of the human body in the direction of the arrow and is circulated outside the body. A state in which the HIV virus contained in the blood or plasma component of whole blood is inactivated by the action of an electric field and an electric current, after passing through blood blood or a plasma component obtained by separating blood, and then returned to the human body. Is shown.

An embodiment of the present invention will be described below with reference to FIG. FIG. 17 is a structural schematic explanatory view showing the means for inactivating the virus of the example of the present embodiment, and FIG. 17 is a vertical sectional view,
In the figure, 1 is a filtering instrument, 2 is an electrode, 3 is a terminal, 4 is a crosspiece, 5 is a filtering material made of a gas permeable conductor, and 7 is a hole.

FIG. 17 is different from the case shown in FIG. 16 in the following points. Inside the filtering instrument 1, the breathable electrodes 6 are provided so as to face each other above and below, and the inside of the facing breathable electrodes 6 is filled with a filter material 5 made of a conductor and brought into contact with the breathable electrodes 6. Filtering device 1
Filtration device 1 having a first electrode and a second electrode, or a laminated electrode in which a plurality of two electrodes each having a pair of the first electrode and the second electrode are laminated in series is provided. Inside, the blood of the human body is guided in the direction of the arrow to the outside of the human body, and the blood of the whole blood or the plasma component that has separated the blood is passed through the extracorporeal circulation. The HIV virus contained therein is inactivated by the action of an electric field and an electric current, and then blood is returned to the human body.

An embodiment of the present invention will be described below with reference to FIG. FIG. 18 is a structural schematic explanatory view showing a virus inactivating means of the embodiment of the present invention, FIG. 18 is a three-dimensional view, and FIGS. 19, 20, 21 and 22 are assembly drawings, and in FIG.
Is a filtering device, 3 is a terminal, 7 is a hole, 8 is an O-ring, 9, 16, 17 and 18 are packings, 14 and 15 are lids, 19 is a bolt, and 20 is a connecting part. is there.

FIG. 18 shows the filtering device 1
A plurality of breathable electrodes 6 and a plurality of O-rings 8 or packings 9 are alternately laminated inside, and a bolt 19 for connecting the connection portion 20 and the terminal 3 provided on the breathable electrode 6 is used. A lid 1 that is fixed and is provided on both sides of the filtration device 1.
If the structure is to be sealed by using the lid 4 and the lid 15, by providing the packings 16, 17 and 18 at the respective joints, the filtration device 1 capable of keeping the inside of the filtration device 1 completely airtight is manufactured. The structure which can be shown is illustrated.

On November 26, 1993, a bacteriology of Nagasaki University was used on November 26, 1993, using a filtering instrument 1 having a structure similar to that shown in FIG. 18 and capable of maintaining a completely airtight state. Professor Miyamoto of the HIV virus research team in the classroom,
Associate Professor Katamine, Taro Yamamoto graduate student, Hitachi Chemical Co., Ltd.,
FIG. 3 is a three-dimensional view of the filtering device 1 when used in an experiment of joint research with Yoshiaki Nagaura, the applicant of the present invention. Regarding the experimental results, when the electrode opening distance was 1 mm, the alternating current was 2 V, and the residence time was 10 minutes, about 80% of the HIV virus could be inactivated. The type A electrode structure described in the report attached to this application document is shown in FIG.
The structure is almost the same as that of the filtering device 1 shown in FIG.

FIGS. 21 and 22 show that
19 and 20 is different from that shown in FIGS. 19 and 20 in that although the lids 14 and 15 are provided on both side surfaces in FIGS. 19 and 20, only one side is shown in FIG. The difference is that the filtering device 1 having a structure in which a lid 14 is provided on the inside is illustrated. When disassembling the filtering device 1, the lids 14 are provided on both sides.
It is more convenient to provide 15 and 15. Furthermore, FIG.
9 and the packing 9 shown in FIG. 21, and FIG.
Also, the thickness of the holes 7 and the number of holes 7 are different from the packing 9 shown in FIG. The thickness of the holes 7 and the number of the holes 7 of the packing 9 shown in FIGS.
The thickness of the holes 7 provided in the breathable electrode 6 is exactly the same as the number of the holes 7.

An embodiment of the present invention will be described below with reference to FIG. FIG. 23 is a schematic structural view showing the means for inactivating the virus of the example of the present embodiment, FIGS. 23 and 28 are three-dimensional views, and FIGS. 24, 25, 26, 27, 29, and 30. 31 and 32 are assembly drawings, in which 1 is a filtering instrument, 6 is a breathable electrode, 7 is a hole, 8 is an O-ring, 9 is a packing, and 21 is a breathable electrode with a terminal. So 2
2 and 23 are lids, 24 are bolts, 25 are nuts,
26 is a bolt.

FIG. 23 shows a plurality of sheets.
After the terminal breathable electrode 21 for wiring and a plurality of O-rings 8 or packing 9 are alternately laminated, and the terminal breathable electrode 21 and the O-rings 8 or packing 9 are alternately laminated one by one, A lid 22 and a lid 23 provided on both side surfaces of the breathable electrode 21 with terminals are replaced by a bolt 24 and a nut 25.
By sealing and forming the filtration device 1
The structure which can make the filter instrument 1 which can maintain a completely airtight state is illustrated.

24, 25, 26, 27 and 2
9, FIG. 30, FIG. 31, and FIG.
FIG. 24, FIG. 25, and FIG. 2 are assembly views of FIG. 23 and FIG.
6, FIG. 27, FIG. 29, FIG. 30, FIG. 31 and FIG. 32 are different only in the number of holes 7 formed in the breathable electrode with terminal 21. Figure 30
In FIG. 26, only one hole 7 is shown, and in FIGS. 26, 27, 31 and 32, the difference is that a plurality of holes 7 are formed. Further, FIG.
The packing 9 shown in FIGS. 26, 29 and 31 and the packing 9 shown in FIGS. 25, 27, 30 and 32 differ in the thickness of the holes 7 and the number of the holes 7. The thickness of the holes 7 and the number of the holes 7 of the packing 9 shown in FIGS. 27 and 32, which are different from each other, are different from those of the holes 7 provided in the breathable electrode 6 and the breathable electrode 21 with terminals. The shape is exactly the same as the number of.

23, 24, 25, 26 and 2
The shape of the terminal 3 integrally formed with the breathable electrode with terminal 21 shown in FIG. 7 may be any shape, and as shown in FIG.
It may be directly connected to the breathable electrode 6.

On February 26, 1994, using a filtering instrument 1 having a structure similar to that shown in FIGS. 23 and 28 and capable of maintaining a completely airtight state, As explained in 18, we are Professor Miyamoto, Associate Professor Katamine, Taro Yamamoto graduate student of the HIV virus research team at Nagasaki University Bacteriology, Hitachi Chemical Co., Ltd., and the applicant of the present invention. FIG. 3 is a three-dimensional view of the filtration device 1 when used in an experiment of a joint research with Yoshiaki Nagaura. Regarding the experimental results, the distance between the electrodes is 0.1 mm, and the alternating current is 0.5 V,
When the residence time of the solution was 1 minute, about 20% of the HIV virus could be inactivated.

An embodiment of the present invention will be described below with reference to FIG. FIG. 33 is a structural outline explanatory view showing a virus inactivating means of the example of the present embodiment, FIG. 33 is a three-dimensional view, FIG. 34 is a top view, FIG. 35 is an assembly view, and FIG. 36 is a vertical sectional view. FIG. 37 is a three-dimensional sectional view, FIG. 38 is a vertical sectional view, and FIG.
9 is a three-dimensional sectional view, in which 1 is a filtering device, 3 is a terminal, 6 is a breathable electrode, 7 is a hole, 8 is an O-ring,
9, 16, 17, and 18 are packings, 10 is an adhesive, 11 is a single-sided adhesive tape, 12 is a double-sided adhesive tape, 13 is an insulating coating layer, 30 is a laminated electrode, 31 is a temperature control tank. , 32 are packings with an adhesive, 33 is a connecting portion, and 37 is a bolt.

FIGS. 33 and 34 show that
A temperature control tank 31 is provided outside the filtration device 1, and in order to control the temperature inside the filtration device 1, cooling water or hot water is circulated inside the temperature control tank 31 to control the temperature inside the filtration device 1. The structure which can be shown is illustrated.

FIG. 35 shows the filtering device 1
36 is a vertical sectional view of the laminated electrode 30 used inside the filtration device 1, FIG. 37 is a three-dimensional sectional view of the laminated electrode 30, and FIG. 38 is a longitudinal sectional view of the laminated electrode 30. 39 is a three-dimensional cross-sectional view of the laminated electrode 30.

FIG. 36 shows that a plurality of air-permeable electrodes 6, the middle of the air-permeable electrodes 6 and the O-ring 8 or the packing 9 or the adhesive 10 or the single-sided adhesive. Using the tape 11, the double-sided adhesive tape 12, the insulating coating layer 13, the adhesive packing 32, the bolt 37, or the like, several to several tens or more breathable electrodes 6 are laminated to form a pair. The structure of the laminated electrode 30 is shown.

The difference between FIGS. 36 and 37 and FIGS. 38 and 39 is that in the laminated electrode 30 shown in FIGS. 36 and 37, the solution flowing inside the laminated electrode 30 does not stagnant in a straight line state. While flowing straight inside the laminated electrode 30, the laminated electrode 30 shown in FIG. 38 and FIG. 39 flows the inside of the laminated electrode 30 because the breathable electrodes 6 are alternately laminated. The structure is different in that the solution flows meandering inside the laminated electrode 30.

As shown in FIG. 36, FIG. 37, FIG. 38, and FIG. 39, the air-permeable electrode 6 is attached to the O-ring 8 or the packing 9 or the adhesive 9 to several tens or more. Using the adhesive 10, the double-sided adhesive tape 12, the packing 32 with adhesive, the bolt 37, or the like, the laminated electrode 30
If it is formed, there are the following advantages. The distance between the electrodes can be taken accurately. The solution flowing inside the laminated electrode 30 can be made to flow straight inside the laminated electrode 30 without stagnation, or the breathable electrodes 6 can be alternately laminated to form a laminated electrode 30.
It is also easy to allow the solution flowing inside to flow while meandering inside the laminated electrode. As shown in the assembly view of FIG. 35, the vertical sectional view of FIG. 41, and the assembly view of FIG. 42, when the laminated electrode 30 is used as the electrode inside the filtration device 1, the lids on both side surfaces of the filtration device 1 are used. 14 and the lid 15 or the packing 17 provided on the lid 14 on one side is used from both sides or one side to easily seal the inside of the laminated electrode 30 and maintain the airtight state inside the laminated electrode 30. Therefore, the terminal 3 and the connection portion 33 connected to the terminal 3 formed on the laminated electrode 30 can be formed.
However, due to water leakage of the solution, the breathable electrode 6 forming the laminated electrode 30 will not cause a short circuit between the upper and lower sides. As shown in the assembly drawings of FIGS. 35 and 42,
Assembly is facilitated by using the cartridge-type laminated electrode 30 that is easy to handle.

An embodiment of the present invention will be described below with reference to FIG. FIG. 40 is a structural outline explanatory view showing a virus inactivating means of the embodiment of the present invention, FIG. 40 is a three-dimensional view, FIG. 41 is a vertical sectional view, FIG. 42 is an assembly drawing, and FIG. 43 is a vertical sectional view. In the figure, FIG. 44 is a three-dimensional cross-sectional view, FIG. 45 is a vertical cross-sectional view, FIG. 46 is a three-dimensional cross-sectional view, in which 1 is a filtering instrument, 3 is a terminal, 6 is a breathable electrode, and 7 is Hole, 8 is o-ring,
9, 16, 17 and 18 are packing, 10 is adhesive, 11 is single-sided adhesive tape, 12 is double-sided adhesive tape,
Reference numeral 13 is an insulating coating layer, 30 is a laminated electrode, 31 is a temperature control tank, 32 is packing with an adhesive, 33 and 35 are connecting portions, 34 is an electrode connecting part, and 37 is a bolt.

41, 42, 43, 44 and 45.
And, as shown in FIG. 46, the breathable electrode 6 is used by using an O-ring 8 or packing 9, adhesive 10 or double-sided adhesive tape 12, insulating coating layer 13, adhesive packing 32 or bolt 37. Then, after forming the laminated electrode 30, the air-permeable electrodes 6 are connected by using the electrode connecting component 34.
Since the laminated electrode 30 and the terminal 3 can be connected by providing only one connecting portion 35 to be connected to the terminal 3 on the terminal 3, the assembly becomes easier than the case shown in FIG. There is. Other functions and effects are the same as those described with reference to FIGS. 33, 34, 35, 36, 37, 38 and 39.

An embodiment of the present invention will be described below with reference to FIG. FIG. 47 is a structural outline explanatory view showing a virus inactivating means of the example of the present embodiment, FIGS. 47 and 48 are three-dimensional views, FIG. 49 is a top view, FIG. 50 is a vertical sectional view, and FIG.
1, 52 and 53 are assembly drawings, in which 1 is a filtering instrument, 9 is a packing, 27 is an electrode with a connecting portion, 28
Is a connecting portion, 29 is a serpentine packing, 31 is a cooling tank, and 36 is a joining part.

FIG. 47 shows the filtering device 1
Inside, a plurality of electrodes 27 with connecting portions and packing 9 or a plurality of packings 9 and serpentine packings 29 are provided in parallel with the longitudinal direction of the flow of the solution, with electrodes 27 with connecting portions and packings 9 or packing 9 and serpentine packings 29. The structure of the filtering instrument 1 which laminated | stacked several sheets is shown.

FIG. 48 shows that the temperature adjusting tanks 31 are provided on both sides of the filtering instrument 1 shown in FIG. ,
By circulating cooling water or warm water inside the temperature control tank 31,
The structure which can temperature-control the inside of the filtering instrument 1 is shown in figure.

FIG. 49 is a top view of the filtering device 1 shown in FIG. 48, showing a structure in which cooling tanks 31 are formed on both sides of the filtering device 1. ing.

FIG. 50 shows the filtering device 1
The electrode 27 with a connecting portion, the packing 9, and the serpentine packing 29, which are the constituent components of the above, are illustrated.

FIG. 51 shows the filtering device 1
The assembly drawing of the filtration instrument 1 which uses the electrode 27 with a connection part and the serpentine type | mold packing 29 which are the components which comprise is shown in figure.

FIG. 52 shows the filtering device 1
Inside, a plurality of electrodes 27 with connecting portions, a plurality of serpentine packings 29, and a plurality of packings 9 are laminated, and a plurality of electrodes 27 with connecting portions, a plurality of serpentine packings 29, and a plurality of packings 9 are laminated in parallel to the longitudinal direction in which the solution flows. FIG. 3 is an assembly diagram of the filtering device 1, illustrating that the contact area of the solution in contact with the electrode 27 with the connecting portion inside the filtering device 1 is enlarged by using the serpentine type packing 29.

FIG. 53 shows an assembly drawing of the filtering instrument 1 using only the electrode 27 with connecting portion and the packing 9.

As shown in FIG. 47, the filtering device 1 in which the connecting portion-equipped electrodes 27 are arranged in the vertical direction and which can maintain a completely airtight state is used in FIG. 18 and FIG. 23, 1994, as described in 23.
According to the result of an experiment conducted on 26th March in collaboration with the HIV virus research team in the Department of Bacteriology, Nagasaki University, the distance between the electrodes was 0.3 mm, the alternating voltage was 1 V, and the solution residence time was 17 minutes. In this case, about 90% of the HIV virus could be inactivated. The type B electrode in the attached report has an electrode structure that is configured by using two electrodes 27 with joint portions and one serpentine type packing 29 and one packing 9 shown in the figure. Although it is slightly different from the content of the drawing shown in FIG. 47, it basically has the same shape as the type B described in the report.

Using the above-described two types of filtration equipment 1 and the filtration equipment 1 having three types of electrode structures of the filtration equipment 1 in which the electrodes 27 with connecting portions are arranged in the vertical direction, HIV virus, voltage and current The following is a summary of the results of the experiment conducted in collaboration with the HIV virus research team in the Department of Bacteriology, Nagasaki University. By making the distance between the electrodes as small as possible and increasing the potential gradient, it is possible to inactivate the HIV virus even in a solution of the electrolyte, using a voltage and current within a range that does not cause electrolysis. The HIV virus is inactivated in proportion to the voltage gradient. The reason why the HIV virus is inactivated is that the HIV virus is RNA or reverse transcriptase or proteolytic enzyme or HI.
Because the envelope or other important part on the surface of V virus is destroyed or damaged by the influence of voltage and current, or chlorine, active oxygen or carboxyl group generated by electrolysis, the HIV virus is Be inactivated. If the potential gradient is high, the HIV virus can be inactivated even at about 1 V. Therefore, by applying a voltage and a current to the blood circulating outside the body, the HIV virus in the blood can be inactivated. Can be inactivated. When the voltage is 1.5 V or higher, the effect of inactivating the HIV virus is reduced because bubbles are generated on the electrode surface and a polarization action occurs, and thus the effective surface area of the electrode is reduced. Although the apparent voltage is high, the actual voltage is low. Therefore, if the voltage is 1.5 V or higher, the effect of inactivating the HIV virus will decrease, so the distance between the electrodes should be as small as possible. do it,
Even with a voltage of about 1.5 V, it is necessary to increase the potential gradient as much as possible.

An embodiment of the present invention will be described below with reference to FIG. FIG. 54 is a structural outline explanatory view showing a virus inactivating means of the example of the present embodiment, FIGS. 54 and 55 are three-dimensional views, and FIGS. 56, 65, 66 and 67 are top views. 57, FIG. 58, FIG. 59, FIG. 60, FIG.
63 and 64 are longitudinal sectional views, FIG. 68 is a side view, and in the drawings, 1 is a filtering instrument, 7 is a hole, 31 is a temperature control tank,
38 and 39 are cylindrical magnets, 40 and 41 are lids,
42, 43 and 44 are bolts, 45 and 46 are terminals, 47, 48, 49, 50 and 51 are packings, 52 is a sleeve provided with a groove 53, 53 is a groove and 5
4 is a sleeve with holes 7, 55 is a sleeve, 56
Is a thermometer, 57 is a spring, and 58 is a joint part.

FIG. 54 shows that a cylindrical magnet 39 having a diameter smaller than that of the cylindrical magnet 38 is inserted into the cylindrical magnet 38 and fixed by using a bolt 42. The lid 40 and lid 41 with bolts 43 and 4
Figure 4 illustrates the structure of a cylindrically shaped filtering device 1 that is sealed and formed using 4 or joints 58.

FIG. 55 shows that the temperature control tank 31 is provided on the outer wall of the filter device 1 shown in FIG. 54 to control the temperature inside the cylindrical magnets 38 and 39. By circulating cooling water or warm water inside the temperature control tank 31, the temperature of blood, plasma components and lymph fluid passing through the inside of the filtering device 1 is constantly kept at a constant temperature, for example, 37 ° C.
The state holding to front and back is illustrated.

FIG. 56 is a top view of the filtering device 1 shown in FIG. 55, which shows a cylindrical magnet 38.
The structure in which the temperature control tank 31 is formed on the outer wall of FIG. As shown in FIG. 60, the temperature adjusting tank 31 may not be provided on the outer wall of the cylindrical magnet 38.

57, 58, 59, 60 and 6
1, FIG. 62, FIG. 63 and FIG.
Inside the cylindrical magnet 38, another cylindrical magnet 39 having a smaller diameter than that of the cylindrical magnet 38 is used by using the sleeve 52 having the groove 53 or the sleeve 54 or the sleeve 55 having the hole 7. The shape-shaped magnet 39 is accurately fixed, and at the same time, it has a cylindrical structure capable of passing a solution such as blood, plasma component and lymph fluid without stagnation.
1 shows a vertical cross-sectional view of a filtering device 1.

FIG. 58, FIG. 59, and FIG. 60 show that the cylindrical magnet 38 is provided inside the cylindrical magnet 38.
Insert another cylindrical magnet 39 with a smaller diameter than
Inserting the sleeve 52 provided with the groove 53 shown in FIG. 63 from both side surfaces, the cylindrical magnet 39 is accurately measured,
It is fixed inside the cylindrical magnet 38, and the lid 40 is attached from both sides.
A vertical cross-sectional view of the filtering device 1 having a cylindrical structure that is hermetically sealed by using a lid 41 and a lid 41 is shown.

In FIG. 61, another cylindrical magnet 39 having a diameter smaller than that of the cylindrical magnet 38 is inserted into the cylindrical magnet 38, and both side surfaces are shown in FIG.
The sleeve 54 with the hole 7 shown in FIG. 4 is inserted, the cylindrical magnet 39 is accurately fixed inside the cylindrical magnet 38, and the lid 40 and the lid 41 are used from both sides. 1 shows a longitudinal sectional view of a filtering device 1 having a closed cylindrical shape.

FIG. 62 shows that another cylindrical magnet 39 having a hole 7 having a smaller diameter than that of the cylindrical magnet 38 is inserted into the cylindrical magnet 38, and both side surfaces are inserted. 66, the sleeve 55 is inserted, the cylindrical magnet 39 provided with the hole 7 is accurately fixed inside the cylindrical magnet 38, and the lids 40 and 41 are used from both side surfaces. FIG. 1 shows a vertical cross-sectional view of the filtering device 1 having a cylindrical structure that is sealed.

FIG. 63 shows another cylindrical magnet 39 in which a groove 53 is provided inside the cylindrical magnet 38.
66, the sleeve 55 shown in FIG. 66 is inserted from both sides, and the cylindrical magnet 39 provided with the groove 53 is accurately fixed inside the cylindrical magnet 38 and the lid is inserted from both sides. A vertical cross-sectional view of the filtering device 1 is shown, which uses a 40 and a lid 41 and has a sealed cylindrical shape.

FIG. 64 shows that another cylindrical magnet 39 is provided inside a cylindrical magnet 38 provided with a groove 53.
65, the sleeve 53 shown in FIG. 65 is inserted from both sides, and the cylindrical magnet 39 is accurately inserted into the groove 5.
3 is a longitudinal cross-sectional view of the filtering instrument 1 having a cylindrical structure which is fixed inside the cylindrical magnet 38 provided with 3 and is sealed by using the lid 40 and the lid 41 from both side surfaces.

FIG. 68 shows the sleeve 52 provided with the groove 53 or the sleeve 54 or sleeve 55 provided with the hole 7 shown in FIGS. 65, 66 and 67.
FIG.

54 and 55, the filtering device 1 having a cylindrical electromagnetic structure, which is formed by inserting the cylindrical magnet 39 into the cylindrical magnet 38, is as follows.
In order to increase the magnetic field strength, even if the distance between the magnets is made as small as possible, the inside of the filtration device 1 can be smoothed without applying excessive pressure to the blood circulated outside the body. It allows blood to pass through.

As described above, FIGS. 3, 4, 5, 6, 6, 8, 11, 14, 15, 16, 17, 18, 19, and 20. 21, FIG. 24, and FIG.
Using the electrodes and magnets of the electrode structure shown in FIG. 3, FIG. 40, FIG. 47, FIG. 48, and FIG. 54, as in the case described in FIG. Oxidation of ammonia dissolved in blood, which occurs as a result of failure, by electrolysis or by the action of electrodes or magnetic fields can be used to oxidize ammonia in blood to nitric acid.

[Effects of the Invention] When a voltage, current or magnetic field is applied to cells, the ion channels on the cell surface or changes in membrane potential or pumps on the cell surface are stimulated to instantaneously open the cell surface. It is possible to change the ion channel or the membrane potential of the cell or the pump on the cell surface,
Opening using a voltage, current or magnetic field stimulation causes the solution outside the cell to move back and forth between the solution inside the cell, eg the Na ions outside the cell When the cells are activated, the K ions inside the cells flow out of the cells, and when the cells are activated, the cells produce and release the satocaines interleukin and interferon. As a result, interleukins and interferons, which are protective substances of the human body, are synthesized in the body.

Although the mechanism of action of interleukins and interferons against HIV virus, hepatitis virus and other viruses has not been fully elucidated yet, cells in which the interleukins and interferons bind to receptors on target cells and receive the information Becomes an antiviral state and inhibits the growth of the virus,
It will suppress the onset of IV virus, hepatitis virus and other viral diseases.

A sandwich in which an insulating layer is formed by using an O-ring or packing made of a gas permeable electrode and an insulator, an adhesive, a single-sided adhesive tape, a double-sided adhesive tape, an insulating coating layer, or a packing with an adhesive. With a filtering device that uses a shaped, breathable, laminated electrode structure, the distance between the electrodes can be made extremely small, and the potential gradient can be easily increased. Therefore, it is possible to inactivate a virus such as HIV virus, hepatitis virus or carcinogenic virus by using a voltage within a range that does not affect the living body.

For example, the HIV virus can be inactivated at a voltage of about 60 V in the case of an alternating current when the distance between electrodes is 1 cm, which is a unit of electricity. The limit which can withstand is AC or DC, 3V is the limit. As a result, the potential gradient between 60V when the distance between electrodes is 1 cm and 3V when the distance between electrodes is 0.5 mm is the same. Therefore, if the voltage between the electrodes is 0.5 mm and a voltage of 3 V is applied, the same effect as 60 V when the distance between the electrodes is 1 cm is produced. From this, it is necessary to reduce the distance between the electrodes as much as possible because the limit of the cells of the living body that can withstand the voltage is about 3V. By using the present invention, even if the distance between the electrodes is made small, a large amount of solution such as blood can pass through, and at the same time, the current is not short-circuited and the voltage can be safely applied to the solution such as blood. Can be applied. However, if a high-voltage pulse is used, a higher voltage can be applied to the blood, and therefore it can be said that the high-voltage pulse is more effective.

According to the results of an experiment conducted on June 14, 1993, in collaboration with Dr. Hiroshi Shiraki of the Fukuoka Blood Center, the same filtering device as that shown in FIG. 18 was used. Then, an experiment was conducted to find out how much V blood can withstand the effects of chlorine, active oxygen, carboxyl groups, etc. generated by voltage, current and electrolysis. The result is that the distance between the electrodes is 0.
It was found that the limit is about 3.5 V for both AC and DC at 5 mm. At 3.5 V or higher, it was found that platelets were first denatured. However, when a high voltage pulse is applied instantaneously, a voltage higher than 3.5 V can be applied.

According to the result of the experiment conducted at Nagasaki University this time, as shown in Table 3 of the attached report, HI
When the distance between the electrodes of the V virus is 0.3 mm and a voltage of 3 V is applied after 120 hours, the number when the electric field treatment is not performed is the HIV virus number is 18,300.
In case of electric field treatment using 3V voltage
The virus number has dropped to 191. The reason why such a revolutionary effect occurs is that water or salt in the electrolyte solution is decomposed by electrolysis, and a very small amount of active oxygen or chlorine is generated on the electrode surface. It can be judged that the HIV virus has been revolutionarily inactivated by the oxidative action or the bactericidal action of chlorine.

From the above, a very small amount of active oxygen or chlorine, which does not affect the human body, is generated on the electrode surface, and a solution such as blood or plasma passes through the electrode. Then, by contacting with blood etc. on the electrode surface and using a very small amount of active oxygen or chlorine generated on the electrode surface or a carboxyl group etc. generated by other electrolysis, blood It is also possible to inactivate HIV virus, hepatitis virus, etc. present in the solution such as. Using the action of voltage or current or the toxicity of active oxygen or chlorine,
When inactivating viruses such as HIV virus, the shape of the electrodes may be any of laminated electrodes, circular electrodes, round electrodes, parallel plate electrodes, and any shape of electrodes.
However, it is only a matter of distance between electrodes, voltage, current and effective surface area of electrodes.

Voltages and currents are used to inactivate viruses such as HIV virus and hepatitis virus, and by adjusting the distance and voltage of electrodes, highly toxic active oxygen or chlorine is removed. By precisely adjusting, the amount of active oxygen or chlorine generated on the electrode surface can be adjusted to the limit by using voltage and current to generate a very small amount of active oxygen or chlorine. Therefore, a virus such as HIV virus can be inactivated by using a very small amount of active oxygen or chlorine within a range that does not affect the human body. In this case, although a direct current may be used as the current to be used, it is possible to accurately adjust the generation amount of chlorine by using alternating current because the electrode does not polarize. The poisonous chlorine can be used for a therapeutic method capable of suppressing or inactivating the poisonous HIV virus.

Blood is obtained by passing a current through the negative electrode or the positive electrode using a filter material made of a gas permeable conductor, which has a spherical shape or porous shape made of a conductive material, or a honeycomb shape or a sponge shape. Or in an electrolyte solution such as plasma or serum components,
Even with a low voltage, the effective surface area of the breathable electrode or electrodes can be increased, so that a large amount of current can be flowed evenly in the solution, and in blood or plasma components separated from blood or serum components. Since it is possible to uniformly inactivate viruses such as the HIV virus existing in the human body, it is impossible to completely eradicate the HIV virus from the human body, but it is possible to inactivate the HIV virus in the blood and It is effective as a therapeutic means by which the onset of AIDS can be delayed by lowering the value of HIV virus.

A conductor material made of pure gold clay, pure silver clay, pure platinum clay, gold, platinum, carbon, activated carbon, titanium, palladium, nickel or the like, and having a porous or honeycomb shape or a sponge shape. By using a breathable electrode made by forming a hole in the electrode, the effective surface area of the electrode can be increased by the synergistic effect of the hole formed in the breathable electrode and the porous hole Even if a low voltage is used in the range that does not cause any of the above, the HIV virus that is non-covalently bound on the surface of the HIV virus in a free state or the infected cell infected with the HIV virus can be transferred to the breathable electrode. It becomes possible to electrically inactivate or remove it on the surface of the human body, and when human blood is extracorporeally circulated via the laminated electrodes, HIV virus or infected cells in the blood can be removed. It is possible to inactivate or remove, can delay the onset of AIDS, there is an effect as a therapeutic means.

When the laminated electrodes are used, the flow velocity necessary for the treatment can be obtained even if the distance between the electrodes is infinitely close to 0 and the solution such as blood is not pressured more than necessary. In addition to virus inactivation, it is a solution of an electrolyte to oxidize the ammonia dissolved in the blood, which occurs as a result of acute liver failure and liver failure, using electrolysis or the oxidative action of electrodes. , In the range where electrolysis does not occur in blood at all, for example, when the distance between electrodes is 0.3 mm, even a low voltage of about 0.5 V,
The actual potential gradient is as high as 16.5 V per cm, which is a unit of electricity, so that an action of oxidizing ammonia in blood to nitric acid occurs. Furthermore, by using the laminated electrode, the effective surface area of the electrode in contact with the solution is remarkably widened, which is more effective.

Contents of the report Examination of HIV-1 inactivation by electric field treatment 1 Tsutomu Miyamoto, 1 Taro Yamamoto, 1 Shigeru Katamine, 2 Yoshiaki Nagaura, 3 Toyoichi Ueda 1 Bacteriology, Nagasaki University 2 Nagaura Research Institute, 3 Hitachi Chemical Co., Ltd. Head Office Management Planning Office

Objective of research HIV-1 is one of the viruses having extremely weak resistance and is known to be easily inactivated by physical treatment such as heating or treatment with a surfactant or various disinfectants. . However, HIV- mixed with biological materials including blood
A method for inactivating 1 without impairing the functions of cells in the material and physiologically active substances (serum proteins, etc.) has not yet been established. HIV is not such a method in the future
-1 It is considered that it will open the way for the development of new treatment and prevention methods for infectious disease (ADIS). We focus on electric field treatment as one of the measures for that, and
The effect on infectivity was examined.

Materials and Methods 1. electrode. The two types of electrodes shown in Table 1 were used for trial purposes for this purpose. 2. HIV-1 electric field treatment. LAV / HIV-1 infected C
An HIV-1 virus stock was prepared from the EM cell culture supernatant. This virus solution was passed through the electrode at a constant speed, during which voltage of various intensities in the range of 0-3.0V (AC,
The virus solution was recovered by energizing at 60 HZ). The temperature inside the electrode was measured with time. 3. Virus recovery rate from the electrode. In order to examine the possibility of virus adsorption to the electrode, the amount of gag protein (p24) in the virus solution collected from the electrode was measured and compared with that of the virus stock stock solution. 4. HIV-1 infectious titer. The collected virus solution was adsorbed to susceptible cells MT4 for 1 hour at 37 ° C., and the unadsorbed virus was thoroughly washed and then cultured. After the culture was started, the supernatant was collected over time, and the amount of p24 therein was measured and used as an index of the infectious titer of the virus solution. 5. Measurement of intracellular invading virus. The collected virus solution was adsorbed to sensitive cells CEM for 1 hour at 37 ° C., immediately treated with 0.025% trypsin, and then the intracellular p24 amount was measured. 6. p24 measurement. Commercially available EIA kit (HIVAG-
1, Dynabot Co., Ltd.) was used. OD492 in advance
The standard curve of p24 was obtained, and the amount of p24 was calculated based on the standard curve.

Results 1. First, a type A electrode (laminated type) was used for examination (Table 2). Passage rate of virus solution through electrode 1.1 ml / mi
m and a residence time of 10.6 min. No increase in temperature (25 ° C.) inside the electrode was observed up to a load voltage of 1.0 V, but it increased to 28, 34, 44 ° C. at 1.5, 2.0 and 2.5 V, respectively. A significant decrease in HIV-1 infectivity was observed at a load voltage of 2.0 V or higher. The virus recovery rate from the electrode did not correlate with HIV-1 in the recovered virus solution, and it was considered that the decrease in HIV-1 infectivity was not due to the adsorption of the virus on the electrode. 2. Next, in order to eliminate the effect of temperature rise, the same study was conducted using a type B electrode (parallel plate type) made of surface gold plated copper having excellent thermal conductivity (Table 3). The electrode was placed in ice under the conditions of a passage speed of 0.13 ml / mim in the electrode and a residence time of 17.5 mim. No temperature increase was observed up to the load voltage of 3.0V. A decrease in infectious titer was already observed at 0.5 V. The level was almost the same up to 2.5V,
At 3.0 V, a more rapid drop was recognized. At 3.0 V, some adsorption of virus on the electrode was also observed. 3. In order to know the effect of the electric field on the initial viral infection process, the amount of intracellular p24 was measured after adsorbing the virus collected from the type B electrode for 1 hour. As a control, a virus treated at 60 ° C. for 10 or 60 mim was used. 2.4% of the untreated virus (p24) was detected from the inside of the cell, but it was dramatically reduced to 0.01% with the virus treated at 60 ° C. for 60 minutes. On the other hand, the electric field treatment virus shows a significant decrease at 1.0 V,
Under other conditions, the degree of decline was small and did not seem to correlate with the degree of enchanting value.

Discussion By passing the HIV-1 virus solution through an electric field, the infectious titer in the solution was reduced. There is a strong possibility that the electric field acts on the virus itself and causes it to lose its infectivity, not due to the adsorption of the virus by the electrodes. In the experiment using the type A electrode, the temperature inside the electrode also rises when the load voltage is increased, so the influence of the temperature should be taken into consideration, but the type B electrode inactivated the virus without increasing the temperature. Further, a detailed analysis of the results suggests that the electric field strength (V / cm) and the residence time in the electrode are important factors for virus inactivation. This time, the electric field treatment is constant H
It seems to have an IV-1 inactivating effect. Dr. Yamamoto's group at Tokyo Medical and Dental University and we use a different system (counter electrode) for HIV by electric field
-1 We have found an inactivating effect (personal communication) and support this result. The elucidation of the mechanism of HIV-1 inactivation by electrolysis is a topic for future research, but our result suggests that the electric field may have a point of action other than the mechanism involved in virus adsorption and entry into cells. Suggestive and interesting.
In addition, it is necessary to take into consideration the electrolysis effect brought about by the high electric field strength. The biggest problem with this result is that the degree of HIV-1 inactivation obtained was smaller than other inactivation treatments (such as heating) and was not perfect. In the future, we plan to set the electric field treatment conditions for the complete inactivation of HIV-1 and develop more efficient electrodes.

[0106]

[0107]

[0108]

[Brief description of drawings]

FIG. 1 is an explanatory view of the principle of the present invention, and FIG. 1 is a vertical sectional view.

FIG. 2 is a schematic structural explanatory view showing a means for inactivating a virus according to an embodiment of the present invention, and FIG. 2 is a vertical sectional view.

FIG. 3 is a schematic structural explanatory view showing a means for inactivating a virus according to an embodiment of the present invention, and FIG. 3 is a vertical sectional view.

FIG. 4 is a structural schematic explanatory view showing a means for inactivating a virus according to an embodiment of the present invention, and FIG. 4 is a vertical sectional view.

FIG. 5 is a schematic structural explanatory view showing a means for inactivating a virus according to an embodiment of the present invention, and FIG. 5 is a vertical sectional view.

FIG. 6 is a schematic structural explanatory view showing a means for inactivating a virus according to an embodiment of the present invention, and FIG. 6 is a vertical sectional view.

FIG. 7 is a schematic structural explanatory view showing a means for inactivating a virus according to an embodiment of the present invention, and FIG. 7 is a vertical sectional view.

FIG. 8 is a schematic structural explanatory view showing a means for inactivating a virus according to an embodiment of the present invention, and FIG. 8 is a vertical sectional view.

FIG. 9 is a schematic structural explanatory view showing a means for inactivating a virus according to the embodiment of the present invention, and FIG. 9 is a vertical sectional view.

FIG. 10 is a schematic structural explanatory view showing a means for inactivating a virus according to an embodiment of the present invention, and FIG. 10 is a vertical sectional view.

FIG. 11 is a structural schematic explanatory view showing a means for inactivating a virus according to the embodiment of the present invention, and FIG. 11 is a vertical sectional view.

FIG. 12 is a structural schematic explanatory view showing a means for inactivating a virus according to the embodiment of the present invention, and FIG. 12 is a vertical sectional view.

FIG. 13 is a structural schematic explanatory view showing a means for inactivating a virus according to the embodiment of the present invention, and FIG. 13 is a vertical sectional view.

FIG. 14 is a schematic structural explanatory view showing a means for inactivating a virus according to the embodiment of the present invention, and FIG. 14 is a vertical sectional view.

FIG. 15 is a schematic structural explanatory view showing a means for inactivating a virus according to an embodiment of the present invention, and FIG. 15 is a vertical sectional view.

FIG. 16 is a structural schematic explanatory view showing a means for inactivating a virus according to an embodiment of the present invention, and FIG. 16 is a vertical sectional view.

FIG. 17 is a structural schematic explanatory view showing a means for inactivating a virus according to the example of the present invention, and FIG. 17 is a vertical sectional view.

FIG. 18 is a schematic structural explanatory view showing a means for inactivating a virus according to an example of the present invention, and FIG. 18 is a three-dimensional view.

FIG. 19 is a schematic structural explanatory view showing a means for inactivating a virus according to the embodiment of the present invention, and FIG. 19 is an assembly drawing.

FIG. 20 is a schematic structural explanatory view showing a means for inactivating a virus according to the embodiment of the present invention, and FIG. 20 is an assembly drawing.

FIG. 21 is a structural schematic explanatory view showing a means for inactivating a virus according to an embodiment of the present invention, and FIG. 21 is an assembly drawing.

22 is a structural schematic explanatory view showing a means for inactivating a virus according to the embodiment of the present invention, and FIG. 22 is an assembly drawing.

FIG. 23 is a schematic structural explanatory view showing a means for inactivating a virus according to an example of the present invention, and FIG. 23 is a three-dimensional view.

FIG. 24 is a schematic structural explanatory view showing a means for inactivating a virus according to the embodiment of the present invention, and FIG. 24 is an assembly drawing.

FIG. 25 is a structural schematic explanatory view showing a means for inactivating a virus according to an embodiment of the present invention, and FIG. 25 is an assembly drawing.

FIG. 26 is a structural schematic explanatory view showing a means for inactivating a virus according to the embodiment of the present invention, and FIG. 26 is an assembly drawing.

FIG. 27 is a structural schematic explanatory view showing a means for inactivating a virus according to the embodiment of the present invention, and FIG. 27 is an assembly drawing.

28 is a schematic structural explanatory view showing a means for inactivating a virus according to the example of the present invention, and FIG. 28 is a three-dimensional view.

FIG. 29 is a structural schematic explanatory view showing a means for inactivating a virus according to the embodiment of the present invention, and FIG. 29 is an assembly drawing.

FIG. 30 is a structural schematic explanatory view showing a means for inactivating a virus according to the embodiment of the present invention, and FIG. 30 is an assembly drawing.

FIG. 31 is a structural schematic explanatory view showing means for inactivating a virus according to the embodiment of the present invention, and FIG. 31 is an assembly drawing.

32 is a structural schematic explanatory view showing a means for inactivating a virus according to the embodiment of the present invention, and FIG. 32 is an assembly drawing.

FIG. 33 is a structural schematic explanatory view showing a means for inactivating a virus according to an example of the present invention, and FIG. 33 is a three-dimensional view.

FIG. 34 is a structural schematic explanatory view showing a means for inactivating a virus according to the example of the present invention, and FIG. 34 is a top view.

FIG. 35 is a structural schematic explanatory view showing a means for inactivating a virus according to the embodiment of the present invention, and FIG. 35 is an assembly drawing.

FIG. 36 is a structural schematic explanatory view showing a means for inactivating a virus according to the example of the present invention, and FIG. 36 is a vertical sectional view.

FIG. 37 is a structural schematic explanatory view showing a means for inactivating a virus according to the example of the present invention, and FIG. 37 is a three-dimensional sectional view.

38 is a structural schematic explanatory view showing a means for inactivating a virus according to the example of the present invention, and FIG. 38 is a longitudinal sectional view.

[Fig. 39] Fig. 39 is a schematic structural explanatory view showing a means for inactivating a virus according to the example of the present invention, and Fig. 39 is a three-dimensional sectional view.

FIG. 40 is a structural schematic explanatory view showing a means for inactivating a virus according to an example of the present invention, and FIG. 40 is a three-dimensional view.

41 is a schematic structural explanatory view showing a means for inactivating a virus according to the example of the present invention, and FIG. 41 is a vertical sectional view.

42 is a schematic structural explanatory view showing a means for inactivating a virus according to the embodiment of the present invention, and FIG. 42 is an assembly drawing.

[Fig. 43] Fig. 43 is a structural schematic explanatory view showing a means for inactivating a virus according to an example of the present invention, and Fig. 43 is a vertical sectional view.

[Fig. 44] Fig. 44 is a structural schematic explanatory view showing a means for inactivating a virus according to an example of the present invention, and Fig. 44 is a three-dimensional cross-sectional view.

FIG. 45 is a structural schematic explanatory view showing a means for inactivating a virus according to an example of the present invention, and FIG. 45 is a vertical sectional view.

FIG. 46 is a schematic structural explanatory view showing a means for inactivating a virus according to the example of the present invention, and FIG. 46 is a three-dimensional sectional view.

[Fig. 47] Fig. 47 is a structural schematic explanatory view showing a means for inactivating a virus according to an example of the present invention, and Fig. 47 is a three-dimensional view.

FIG. 48 is a structural schematic explanatory view showing a means for inactivating a virus according to the example of the present invention, and FIG. 48 is a three-dimensional view.

FIG. 49 is a structural schematic view showing a means for inactivating a virus according to the example of the present invention, and FIG. 49 is a top view.

50 is a structural schematic explanatory view showing a means for inactivating a virus according to an example of the present invention, and FIG. 50 is a vertical sectional view.

51 is a schematic structural explanatory view showing a means for inactivating a virus according to the embodiment of the present invention, and FIG. 51 is an assembly drawing.

52 is a schematic structural explanatory view showing a means for inactivating a virus according to the embodiment of the present invention, and FIG. 52 is an assembly drawing.

53 is a structural schematic explanatory view showing a means for inactivating a virus according to the embodiment of the present invention, and FIG. 53 is an assembly drawing.

54 is a schematic structural explanatory view showing a means for inactivating a virus according to an example of the present invention, and FIG. 54 is a three-dimensional view.

55 is a structural schematic explanatory view showing a means for inactivating a virus according to an example of the present invention, and FIG. 55 is a three-dimensional view.

FIG. 56 is a structural schematic explanatory view showing a means for inactivating a virus according to the example of the present invention, and FIG. 56 is a top view.

FIG. 57 is a structural schematic explanatory view showing a means for inactivating a virus according to the example of the present invention, and FIG. 57 is a vertical sectional view.

58 is a structural schematic explanatory view showing a means for inactivating a virus according to the example of the present invention, and FIG. 58 is a vertical cross-sectional view.

FIG. 59 is a structural schematic explanatory view showing a means for inactivating a virus according to the example of the present invention, and FIG. 59 is a vertical sectional view.

FIG. 60 is a schematic structural explanatory view showing a means for inactivating a virus according to the example of the present invention, and FIG. 60 is a longitudinal sectional view.

FIG. 61 is a schematic structural explanatory view showing a means for inactivating a virus according to the example of the present invention, and FIG. 61 is a vertical sectional view.

62 is a structural schematic explanatory view showing a means for inactivating a virus according to the example of the present invention, and FIG. 62 is a vertical sectional view.

FIG. 63 is a structural schematic explanatory view showing a means for inactivating a virus according to the example of the present invention, and FIG. 63 is a vertical sectional view.

64 is a structural schematic explanatory view showing a means for inactivating a virus according to an example of the present invention, and FIG. 64 is a vertical sectional view.

FIG. 65 is a schematic structural explanatory view showing a means for inactivating a virus according to the example of the present invention, and FIG. 65 is a top view.

FIG. 66 is a structural schematic explanatory view showing a means for inactivating a virus according to the example of the present invention, and FIG. 66 is a top view.

67 is a schematic structural explanatory view showing a means for inactivating a virus according to the example of the present invention, and FIG. 67 is a top view.

68 is a structural schematic explanatory view showing a means for inactivating a virus according to the example of the present invention, and FIG. 68 is a side view.

[Explanation of symbols]

1. 1. A filtering device made of an insulating material such as glass, plastic or metal (abbreviated as a filtering device) 1. 2. An electrode made of gold, platinum, carbon, graphite, activated carbon, titanium, palladium, nickel, or the like (abbreviated as an electrode). Terminal 4. Crosspiece made of plastic (abbreviated as crosspiece) 5. 5. A filter material made of a breathable conductor (abbreviated as a filter material made of a breathable conductor or a filter material) 6. 7. A breathable electrode made of gold, platinum, carbon, graphite, activated carbon, titanium, palladium, nickel, aluminum, or the like (abbreviated as a breathable electrode) 7. Hole 8. O-rings made of insulators (O-rings for short) 9. Packing made of insulator (abbreviated as packing) 10. 11. Adhesive made of non-conductive material (abbreviated as adhesive) 11. One-sided adhesive tape made of non-conductive material (abbreviated as
Single-sided adhesive tape) 12. Double-sided adhesive tape made of non-conductive material (abbreviated as
Double-sided adhesive tape) 13. Coating layer coated with insulating coating material (abbreviated as insulating coating layer) 14 and 15. 16. Lids on both sides of the filtering device 1 (abbreviated as lids) 16 and 17. 17. Packing provided on both sides of the filtering device 1 (abbreviated as packing) 18. 18. Packing for maintaining airtightness of the screw 20 (abbreviated as packing) 19. A bolt for fixing the terminal 3 (abbreviated as a bolt) 20. 20. Connection part provided on the breathable electrode 6 (abbreviated as a connection part) 21. Breathable electrode that integrally forms the terminal 3 (abbreviated as
Air-permeable electrode with terminal) 22 and 23. 24. Lids provided on both side surfaces of the filtering instrument 1 (abbreviated as lids) 24. 25. Bolts whose surface is insulated or bolts made of an insulator (abbreviated as bolts) 25. 26. A nut whose surface is insulation-treated or a nut made of an insulator (abbreviated as nut) 26. Bolt 27. Electrodes provided with connection portions 28 (abbreviated as electrodes with connection portions) 28. Connection part 29. A serpentine packing for causing the solution to meander inside the electrode 27 with a connecting portion (abbreviated as serpentine packing) 30. Laminated electrode 31. Temperature control tank 32. 33. Packing with adhesive applied on both sides (abbreviated as packing with adhesive) 33. 34. A connecting portion for connecting the laminated electrode 30 and the terminal 3 (abbreviated as a connecting portion) 34. 35. Electrode connection component for making each breathable electrode 6 into a pair (abbreviated as electrode connection component) 35. 36. A connection portion provided on the electrode connection component (abbreviated as a connection portion) 36. Joint parts for branching and joining the solution (abbreviated as joint parts) 37. 38. Bolts and nuts whose surface is insulated or bolts and nuts made of an insulator (abbreviated as bolts) 38. 39. A magnet having a cylindrical shape or other shape made of an electromagnet or a permanent magnet such as ferrite (abbreviated as a cylindrical magnet) 39. 40. A cylindrical magnet having a diameter smaller than that of a cylindrical magnet 38 made of an electromagnet or a permanent magnet such as ferrite, or a magnet having another shape (abbreviated as a cylindrical magnet) 40) and 41. 42.43 and 44. A lid or a lid made of an insulating material whose surface has been subjected to an insulation treatment for forming the filtering device 1 42.43 and 44. Bolts whose surfaces are insulated or bolts made of an insulator (abbreviated as bolts) 45 and 46. Fixing bolts or terminals that also serve as terminals (abbreviated as terminals) 47, 48, 49, 50 and 51 Packing or O-rings made of an insulator (abbreviated as packing) 52 Grooves 53 Sleeve made of insulator provided (abbreviated as sleeve provided with groove 53) 53 Groove 54 Sleeve made of insulator provided with hole 7 (abbreviated as sleeve provided with hole 7) 55 Sleeve made of insulator (abbreviated as sleeve) 56 Thermometer 57 Conductive spring (abbreviated as spring) 58 Joint part

Claims (36)

[Claims] 1. A breathable electrode (6) inside a filtration device (1).
Alternatively, a breathable electrode (21) with a terminal or an electrode (27) with a connecting portion is provided so that the distance between the breathable electrode (6), the breathable electrode with a terminal (21) or the electrode with a connecting portion (27) is reduced. 3 mm or 2 mm or 1 mm or 0.5 mm or 0.3 mm or 0.1 mm or 0.05 mm or less, and the voltage or current used has high voltage pulse or direct current or alternating current, tens of thousands of volts or thousands of volts Or hundreds of volts or tens of volts
Alternatively, a solution such as blood or plasma of a human body is passed through the interior of electrodes having a high potential gradient, which is applied with a voltage of several V or 3 V or 2 V or 1 V or 0.5 V or 0.25 V or less, and HI existing in the solution due to direct influence of electric current or indirect influence of chlorine generated by electrolysis.
The HIV virus is inactivated by damaging or destroying the RNA or reverse transcriptase or proteolytic enzyme of V virus or the envelope or other important part of the HIV virus on the surface of the HIV virus. Alternatively, a method for inactivating a virus, which comprises oxidizing ammonia to nitric acid by electrolysis or oxidizing action of an electrode. 2. An electrode and an electrode in which blood of a human body is circulated extracorporeally, and blood which is circulated extracorporeally is applied with a voltage or magnetic field higher than a voltage within a range that does not cause electrolysis or a voltage that causes electrolysis. Alternatively, the virus such as HIV virus and hepatitis virus present in blood and plasma components is passed through between magnets to inactivate them by using the action of voltage and current or magnetic field, and then returned to the human body. A method for inactivating the virus according to claim (1). 3. A sleeve in which another cylindrical electrode (39) having a smaller diameter than that of the cylindrical electrode (38) is inserted into the cylindrical electrode (38) and grooves (53) are provided on both side surfaces. Cylindrical electrode (39) using sleeve (54) or sleeve (55) provided with (52) or hole (7)
A solution such as blood or plasma component of human body or lymph fluid is passed through the inside of the filtration device (1) to which is precisely fixed to inactivate viruses existing in blood and the like. Alternatively, the method for inactivating a virus according to claim 1 or 2, wherein ammonia is oxidized. 4. A cooling tank (31) is provided on the outer wall portion of the cylindrical electrode (38), and cooling water is circulated inside the cooling tank (31) to cool the inside of the filtering device (1). A method for inactivating the virus according to items (1), (2) and (3). 5. A plurality of breathable electrodes (6) and a plurality of O-rings (8) provided with a connecting portion (20) for connecting with a terminal (3) inside a filtering device (1). The packing (9) is alternately laminated, and the terminal (3) is connected to the breathable electrode (6) with the bolt (19) and fixed to the connecting portion (20) provided in the breathable electrode (6). And filtering equipment (1)
A filtering instrument (1) in which the inside is completely sealed by using a lid (14) and a lid (15) provided on both sides of the filter or a lid (14) provided on only one side surface. ) A solution such as blood or plasma of the human body is passed inside to inactivate viruses existing in blood and the like. Alternatively, the method for inactivating a virus according to any one of claims (1), (2), (3) and (4), which comprises oxidizing ammonia. 6. A plurality of breathable electrodes (6) and a plurality of O-rings (8) or packings (9) are alternately laminated and used, or an adhesive (10) or a single-sided adhesive tape (1).
1) or double-sided adhesive tape (12) or insulating coating layer (1)
3) or a packing (32) with an adhesive is used or formed to form an insulating layer on the surface of the breathable electrode (6), and then the lid (22) and the lid (22) provided on both side surfaces. 23)
Using a bolt (24) and a nut (25), a solution such as blood or plasma of the human body is passed through the inside of the filtration device (1) in which the inside of the filtration device (1) is completely sealed, and the like. Inactivate the virus present in. Alternatively, a method for inactivating a virus according to any one of claims (1), (2), (3), (4) and (5), which comprises oxidizing ammonia. 7. A breathable electrode (21) with a terminal, wherein a plurality of breathable electrodes (6) are integrally formed with wiring terminals (3).
And a plurality of O-rings (8) or packings (9) are alternately laminated or used, or an adhesive (10) or a single-sided adhesive tape (11) or a double-sided adhesive tape (12) or an insulating coating layer (13) ) Or using a packing (32) with an adhesive or the like, and forming an insulating layer between the breathable electrode (21) with a terminal and the breathable electrode (21) with a terminal, and then providing it on both sides Using the lid (22) and the lid (23), the bolt (24) and the nut (25), the blood of the human body or the inside of the filter (1) is completely sealed. A virus such as blood is inactivated by passing a solution such as plasma. Or the method of oxidizing ammonia (1), (2), (3), (4),
A method for inactivating the virus according to (5) and (6). 8. A plurality of breathable electrodes (6), wherein an adhesive (10) is provided between the breathable electrodes (6) and the breathable electrodes (6).
Or single-sided adhesive tape (11) or double-sided adhesive tape (1
2) or using an insulating coating layer (13) or packing (32) with adhesive, or using O-rings (8) or packing (9) and bolts (37) to attach a plurality of breathable electrodes. A replaceable cassette type laminated electrode (30), which is formed by laminating several to several tens or more sheets, is placed inside the filtration device (1), and the lids (14) on both sides are inserted. A packing (17) provided on the lid (15) or the lid (14) on one side is used from both sides or one side to seal both sides of the laminated electrode (30), and the laminated electrode (30). Maintains internal airtightness. Further, the packing (16) is used to connect the terminal (3) provided on the laminated electrode (30) and the terminal (3) to the connection portion (33).
A solution such as blood or plasma of the human body is passed through the inside of the filtration device (1) which maintains the airtightness of (1) to inactivate the virus existing in the blood or the like. Further, the method of oxidizing ammonia (1), (2), (3), (4), (5), (6).
And a method for inactivating the virus according to (7). 9. O-ring (8) or packing (9) or adhesive (10) or single-sided adhesive tape (11) or double-sided adhesive tape (12) or insulating coating layer (13) or adhesive-equipped packing (32). Alternatively, when the laminated electrode (30) is formed using bolts (37) or the like, the positions of the holes (7) provided in the air-permeable electrode (6) are aligned with each other so that the holes (7) are aligned. To the laminated electrode (3
0) The laminated electrode (30) in which a plurality of breathable electrodes (6) are laminated so that the solution flowing inside flows straightly and straightly inside the laminated electrode (30) without stagnation, is used as a filter device (1). ) Claims (1), (2), which are used internally
A method for inactivating the virus according to (3), (4), (5), (6), (7) and (8). 10. O-ring (8) or packing (9)
Alternatively, an adhesive (10), a single-sided adhesive tape (11), a double-sided adhesive tape (12), an insulating coating layer (13), a packing with an adhesive (32), a bolt (37), or the like is used to form a laminated electrode (30). ) Is formed, the positions of the holes (7) provided in the breathable electrode (6) are alternately provided to stack the breathable electrodes (6), and the solution flowing inside the stacked electrode (30) is A laminated electrode (30), wherein a plurality of gas permeable electrodes (6) are laminated so as to flow in a zigzag manner inside the laminated electrode (30), and the laminated electrode (30) is used inside a filtration device (1). (2), (3), (4), (5),
A method for inactivating the virus according to (6), (7), (8) and (9). 11. A filter device (1) having two connecting electrodes (27) and one packing (9) or one serpentine packing (in parallel to the longitudinal direction in which a solution flows). Two
9) or a plurality of electrodes with connecting parts (27) and a plurality of packings (9) or a plurality of snake-like packings (29) are alternately laminated, inside the filtration device (1), blood or plasma of a human body. The virus present in blood or the like is inactivated by passing such a solution. Further, the method of oxidizing ammonia (1), (2), (3), (4), (5), (6),
A method for inactivating the virus according to (7), (8), (9) and (10). 12. An electrode (27) having a plurality of connecting portions, which is parallel to the longitudinal direction in which a solution flows, inside the filtering device (1).
A plurality of packings (9) and a plurality of packings (9) are alternately used inside the filtration device (1), and a serpentine packing (29) for increasing the distance through which the solution inside the electrode (27) with a connecting portion flows. (1), (2), (3), (4), which is used inside the electrode (27) with a connecting portion.
A method for inactivating the virus according to (5), (6), (7), (8), (9), (10) and (11). 13. A filter material (5) made of a gas permeable conductor, which is filled with a filter material (5) made of a gas permeable conductor inside a filter device (1) provided with terminals (3) facing each other. Using the filtration device (1) filled with (5), tap water,
Claims (1), (2), (3), which sterilize and inactivate or oxidize microorganisms such as bacteria and viruses or ammonia, bilirubin, toxic substances and the like present in seawater, blood, plasma, serum or other solutions. (4), (5), (6),
(7), (8), (9), (10), (11) and (1
2) A method of inactivating the virus described. 14. A filter device (1) is provided with a terminal (3), and the inside of the filter device (1) is filled with a filter material (5) made of a gas permeable conductor, so that the filter device (1) is made of a gas permeable conductor. Filter material (5)
Into the inside of the filtering instrument (1) in which a negative electrode or positive electrode current is passed through and a negative electrode or positive electrode current is passed through a filter material (5) made of a gas permeable conductor, tap water, seawater, blood, plasma, After passing serum or other solution, microorganisms such as bacteria and viruses present in the solution or ammonia, bilirubin,
Claim (1) which sterilizes and inactivates or oxidizes poisonous substances,
(2), (3), (4), (5), (6), (7),
A method for inactivating the virus according to (8), (9), (10), (11), (12) and (13). 15. A filter material (5) made of a gas permeable conductor between electrodes (2) and electrodes (2) facing each other, provided with electrodes (2) facing each other inside a filtering device (1). ), And the filter material (5) made of a gas permeable conductor is brought into contact with the electrode (2) facing each other and the filter material (5) made of a gas permeable conductor.
Microorganisms such as bacteria, viruses, etc. existing in the solution by passing tap water, seawater, blood, plasma, serum or other solution through the inside of the filtering instrument (1), which is passing the current of the negative electrode or the positive electrode into Alternatively, sterilize and inactivate or oxidize ammonia, bilirubin, poisons, etc. (1), (2),
(3), (4), (5), (6), (7), (8),
A method for inactivating the virus according to (9), (10), (11), (12), (13) and (14). 16. A breathable electrode (6) inside a filtering device (1).
Are provided so as to face each other, and a filtering material (5) made of a breathable conductor is filled between the facing breathable electrodes (6) and (6) to face each other. The inside of the filtration device (1) in which (6) is brought into contact with the filter medium (5) made of a gas permeable conductor and a negative or positive current is passed through the filter medium (5) made of a gas permeable conductor. Water, tap water, seawater, blood, plasma, serum or other solutions to pass through, bacteria, viruses and other microorganisms present in the solution, or ammonia,
Claims (1), (2), (3), (4), (5), which sterilize and inactivate or oxidize bilirubin, poisons and the like.
(6), (7), (8), (9), (10), (1
A method for inactivating the virus according to 1), 12), 13), 14) and 15). 17. A breathable electrode (6) inside a filtering device (1).
Microorganisms such as bacteria and viruses present in the solution by passing water, tap water, seawater, blood, plasma, serum or other solutions through the inside of the filtration device (1) having one or more sheets. Alternatively, sterilize and inactivate or oxidize ammonia, bilirubin, poisons, etc. (1), (2),
(3), (4), (5), (6), (7), (8),
(9), (10), (11), (12), (13),
A method for inactivating the virus according to (14), (15) and (16). 18. Between the electrodes, the blood, plasma component or lymph of the human body is drawn out of the body and circulated outside the body, and the blood circulated outside the body is applied with a voltage higher than the voltage causing electrolysis. For the purpose of neutralizing the toxicity such as chlorine generated by electrolysis, the neutralization of the toxicity such as chlorine in the solution such as blood, plasma component or lymph that has passed between the electrodes. A neutralizer, for example, hypo (sodium thiosulfate), is used to neutralize toxicity of chlorine and the like generated in a solution such as blood, plasma component or lymph, and the like (1), (2), ( 3), (4),
(5), (6), (7), (8), (9), (10),
(11), (12), (13), (14), (15),
A method for inactivating the virus according to (16) and (17). 19. Another breathable electrode (6) provided inside a filtering device (1) so as to face the breathable electrode (6).
Are laminated with O-ring (8) or packing (9) and bolt (37), or adhesive (10) or single-sided adhesive tape (11) or double-sided adhesive tape (12)
Or insulation coating layer (13) or packing with adhesive (3
2) or the like is used to form an insulating layer between the breathable electrode (6) and the breathable electrode (6) and stack the two or more breathable electrodes (6) Bacteria and viruses existing in the solution by passing water and tap water, seawater, blood, plasma, serum or other solutions through the inside of the filtration device (1) provided with the pair of laminated electrodes (30). (1), (2), (3), which sterilize and inactivate or oxidize microorganisms such as or ammonia, bilirubin, poisons, etc.
(4), (5), (6), (7), (8), (9),
(10), (11), (12), (13), (14),
A method for inactivating the virus according to (15), (16), (17) and (18). 20. A breathable electrode (6) inside a filtering device (1).
Are provided so as to face each other, and a filtering material (5) made of a breathable conductor is filled between the facing breathable electrodes (6) and (6) to face each other. The inside of the filtration device (1) in which (6) is brought into contact with the filter medium (5) made of a gas permeable conductor and a negative or positive current is passed through the filter medium (5) made of a gas permeable conductor. The exhaust gas exhausted from an automobile engine or the like is passed through, and nitrogen oxides or sulfur oxides or black smoke contained in the exhaust gas are adsorbed and removed (1), (2), ( 3), (4), (5),
(6), (7), (8), (9), (10), (1
1), (12), (13), (14), (15), (1
6) A method for inactivating the virus according to (17), (18) and (19). 21. The blood of the human body is led from the blood vessel or the lymph fluid to the outside of the human body to be circulated outside the body,
Blood or plasma components or lymph that are circulated outside the body,
An ion channel on the surface of cells such as white blood cells that passes between electrodes or electrodes or magnets and is subjected to a voltage or magnetic field higher than the voltage that does not cause electrolysis or the voltage that causes electrolysis. Alternatively, a change in cell membrane potential or a pump on the cell surface is stimulated to forcefully open the cell by the action of voltage, current or magnetic field, and activates the cell to produce or release cytokines interleukin and interferon. Claims (1), (2),
(3), (4), (5), (6), (7), (8),
(9), (10), (11), (12), (13),
(14), (15), (16), (17), 18),
A method for inactivating the virus according to (19) and (20). 22. Spherical particles or granules made of gold, platinum, carbon, graphite, activated carbon, titanium, palladium, nickel, or the like are used as the filtering material (5) made of a breathable conductor. (1), (2),
(3), (4), (5), (6), (7), (8),
(9), (10), (11), (12), (13),
(14), (15), (16), (17), (18),
A method for inactivating the virus according to (19), (20) and (21). 23. A filter material (5) made of a gas permeable conductor, which is made of gold, platinum, carbon, graphite, activated carbon, titanium, palladium, nickel or the like, and has a porous or honeycomb shape or a sponge shape. (1), (2), (3), (4), which uses an object made of a conductive material,
(5), (6), (7), (8), (9), (10),
(11), (12), (13), (14), (15),
(16), (17), (18), (19), (20),
A method for inactivating the virus according to (21) or (22). 24. A carbon fiber is used as the filter material (5) made of a gas permeable conductor (1), (2),
(3), (4), (5), (6), (7), (8),
(9), (10), (11), (12), (13),
(14), (15), (16), (17), (18),
(19), (20), (21), (22) and (23)
A method for inactivating the described virus. 25. The surface of a spherical or porous, honeycomb-shaped or sponge-shaped filter material (5) used as a filter material (5) made of a breathable conductor is made biocompatible. A filter material (5) made of an electric conductor, which is surface-treated using a resin or an insulating resin to reduce the conductive property of the electric conductor as a filter material (5) made of an electric conductor. Claims to be used (1), (2), (3), (4), (5),
(6), (7), (8), (9), (10), (1
1), (12), (13), (14), (15), (1
6), (17), (18), (19), (20), (2
A method for inactivating the virus according to 1), (22), (23) and (24). 26. One breathable electrode (6) formed by forming holes (7) in a porous material formed by molding pure gold clay, pure silver clay, pure platinum clay, etc. and firing. Alternatively, a plurality of filtering instruments (1) are used to electrically inactivate HIV virus present in blood or the like. Further, the method of oxidizing ammonia (1), (2), (3), (4),
(5), (6), (7), (8), (9), (10),
(11), (12), (13), (14), (15),
(16), (17), (18), (19), (20),
(21), (22), (23), (24) and (25)
A method for inactivating the described virus. 27. One breathable electrode (6) formed by forming holes (7) in a porous material formed by molding pure gold clay, pure silver clay, pure platinum clay, etc. and firing. Alternatively, an HIV virus present on the surface of an infected cell that is non-covalently bound to the surface of the cell is electrically inactivated by using a plurality of filtration devices (1). , (2),
(3), (4), (5), (6), (7), (8),
(9), (10), (11), (12), (13),
(14), (15), (16), (17), (18),
(19), (20), (21), (22), (23),
A method for inactivating the virus according to (24), (25) and (26). 28. An electrically conductive air-permeable electrode (6) formed by forming holes (7) in a porous material made by molding pure gold clay, pure silver clay, pure platinum clay, etc. and firing. (1), (2), which is used as a filter paper having various characteristics,
(3), (4), (5), (6), (7), (8),
(9), (10), (11), (12), (13),
(14), (15), (16), (17), (18),
(19), (20), (21), (22), (23),
A method for inactivating the virus according to (24), (25), (26) and (27). 29. The breathable electrode (6) is made of pure gold clay, pure silver clay, pure platinum clay or the like and is made of a material having a large number of porous holes formed by firing, gold, silver and A breathable electrode (6) formed by forming a hole (7) in platinum or the like is used (1), (2), (3), (4),
(5), (6), (7), (8), (9), (10),
(11), (12), (13), (14), (15),
(16), (17), (18), (19), (20),
(21), (22), (23), (24), (25),
A method for inactivating the virus according to (26), (27) and (28). 30. A porous or honeycomb-shaped or sponge-shaped electrically conductive electrode (6) made of gold, platinum, carbon, graphite, activated carbon, titanium, palladium, nickel or aluminum, etc. Claims (1), (2), (3), (4) using body objects
(5), (6), (7), (8), (9), (10),
(11), (12), (13), (14), (15),
(16), (17), (18), (19), (20),
(21), (22), (23), (24), (25),
A method for inactivating the virus according to (26), (27), (28) and (29). 31. After molding a non-conductive or conductive synthetic resin or metal or non-ferrous metal as the breathable electrode (6),
Use of a conductor object made by electroplating, electroplating or vapor deposition using gold, platinum or the like (1), (2), (3), (4), (5), ( 6),
(7), (8), (9), (10), (11), (1
2), (13), (14), (15), (16), (1
7), (18), (19), (20), (21), (2
2), (23), (24), (25), (26), (2
A method for inactivating the virus according to 7), (28), (29) and (30). 32. A filter device (1) has a double structure on the outside, and a temperature control (31) is provided on an outer wall portion of the filter device (1). Cooling water or hot water is provided inside the temperature control (31). The inside of the filtration device (1) is circulated to cool the inside of the filtration device (1),
(2), (3), (4), (5), (6), (7),
(8), (9), (10), (11), (12), (1
3), (14), (15), (16), (17), (1
8), (19), (20), (21), (22), (2
3), (24), (25), (26), (27), (2
8) A method for inactivating the virus according to (29), (30) and (31). 33. A few to several tens or more breathable electrodes (6) are attached to an O-ring (8) or packing (9) or adhesive (10) or single-sided adhesive tape (11).
Alternatively, the laminated electrode (30) laminated by using the double-sided adhesive tape (12), the insulating coating layer (13), the packing with adhesive (32), the bolt (37), or the like, is a filtration device (1).
Claims (1), (2), (3), which are used internally
(4), (5), (6), (7), (8), (9),
(10), (11), (12), (13), (14),
(15), (16), (17), (18), (19),
(20), (21), (22), (23), (24),
(25), (26), (27), (28), (29),
A method for inactivating the virus according to (30), (31) and (32). 34. A solution, which is formed by using a plurality of electrodes (27) with connecting parts, and which passes through the inside of a filtering device (1), is introduced into the inlet and outlet parts of each electrode (27) with connecting parts. A method of branching and merging a solution by using a connecting part (36) for branching the solution and for merging the solution, (1), (2), (3), (4),
(5), (6), (7), (8), (9), (10),
(11), (12), (13), (14), (15),
(16), (17), (18), (19), (20),
(21), (22), (23), (24), (25),
(26), (27), (28), (29), (30),
A method for inactivating the virus according to (31), (32) and (33). 35. The voltage or current used is a high voltage pulse, a low voltage pulse, an arbitrary pulse waveform, a bipolar pulse, or an alternating current or a direct current or other alternating current superposed with a direct current,
Claims (1), (2), (3), (4), (5), (6), which use a voltage or current having the property of direct current.
(7), (8), (9), (10), (11), (1
2), (13), (14), (15), (16), (1
7), (18), (19), (20), (21), (2
2), (23), (24), (25), (26), (2
7), (29), (30), (31), (32), (3
A method for inactivating the virus according to 3) or (34). 36. In order to produce a safe blood product, a blood plasma component separated from blood is passed between electrodes to which a voltage is applied, and HIV virus, hepatitis virus, present in the plasma component, Claims (1), (2), (3), (4) for the purpose of producing a safe blood product by inactivating carcinogenic viruses and other viruses.
(5), (6), (7), (8), (9), (10),
(11), (12), (13), (14), (15),
(16), (17), (18), (19), (20),
(21), (22), (23), (24), (25),
(26), (27), (28), (29), (30),
(31), (32), (33), (34), and (3
5) A method of inactivating the virus according to item 1.