JPH09196909A - Preservation of collected urine and urine collecting kit - Google Patents

Abstract

PROBLEM TO BE SOLVED: To preserve or transfer collected urine while preventing the denaturalization or increase and decrease of dissolved components in urine by adding a acid of every kind to collected urine to adjust the pH of urine to a specified value range and adding an antibacterial agent, a fluorine compd. and an oxidation inhibitor to collected urine. SOLUTION: The urine collecting kit consists of an almost cylindrical urine collecting holder 1, the test tube 2 inserted into the holder 1 at a time of the collection of urine and a bottomed cylindrical urine collecting cup 3 and a urine collecting jig 4 is attached to the leading end part 21a of the holder 1. The test tube 2 is hermetically sealed by a cap 2a composed of an elastic material and internally held to a predetermined depressurized state. Further, an indication band 2b is provided to the proper place of the test tube 2. The test tube 2 is preliminarily packed with a pH adjusting acid, an antibacterial agent, a fluorine compd. and an oxidation inhibitor. As the pH adjusting acid, for example, granular citric acid is used and the pH of collected urine is adjusted to 4.5-6.5.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method for preserving collected urine capable of transferring collected urine while preventing denaturation and increase / decrease of dissolved components in urine, and a urine collecting kit suitable for realizing this method of preservation. Regarding

[0002]

2. Description of the Related Art Generally, among urination actions that occur several times a day, a part of urine (partial urine) is often collected during a certain urination period and subjected to various tests. Since quantitative values of various measurement items for this partial urine have large urine volume errors, such as urine concentration / thinness depending on the time of urination,
Various corrections have been made. Typically, creatinine is the measurement target, and the target component and creatinine are measured in the partial urine collected for the purpose of the test, and the target component concentration is calculated by converting it to 1 g of creatinine.

[0003]

However, in particular,
In the case of measuring the target component as a mass screening, the correction using creatinine as a measurement target may not function properly. Further, conventionally, there has been no suitable urine collecting kit that can reliably collect a predetermined amount of partial urine and that can transfer the collected urine while preventing denaturation or increase / decrease of dissolved components in urine. The present invention has been made in view of these circumstances, and it is possible to store or transfer the collected urine while preventing the denaturation or increase / decrease of dissolved components in urine, and to accurately detect the concentration of the target component by measuring creatinine. The purpose is to provide a method. Another object of the present invention is to provide a urine collection kit that is suitable for realizing this method and that can collect a predetermined amount of partial urine easily and reliably.

[0004]

[Means for Solving the Problems] In order to achieve the above object, as a result of various investigations, when the collected urine is left at a high temperature of 37 ° C. or higher for a long time, the resident component in the urine undergoes an oxidation reaction. It was confirmed that the creatinine level decreased due to the oxidization of creatinine into creatol or methylguanidine. For example, when the target component is measured as a mass examination, the collected urine may be mailed, and the temperature inside the post may be 50 ° C during the high temperature period.
Since the temperature may reach to 70 ° C, it is sufficiently possible that the collected urine is left at a high temperature of 37 ° C or higher for a long time. Therefore, in the present invention, in order to cope with such a situation, an acid for pH adjustment, an antibacterial drug, a fluorine compound, and an antioxidant are added to the collected urine. When various acids are added to the collected urine to adjust the pH to 4.5 to 6.5, and the collected urine is preserved by adding an antibacterial agent and a predetermined amount of a fluorine compound, C-peptide, albumin, glycine, It is possible to prevent denaturation and increase / decrease of urinary dissolved components such as urea and glucose (see Japanese Patent Application No. 5-5675). Further, in the present invention, since an antioxidant is further added to the collected urine, the concentration of the target component can be accurately detected by using creatinine as a measurement target.

[0005]

BEST MODE FOR CARRYING OUT THE INVENTION The present invention will now be described in more detail with reference to the drawings. FIG. 1 is a perspective view illustrating components of a urine collecting kit according to the present invention, and FIG. 2 is a diagram illustrating a usage (a) of the urine collecting kit and details (b) of the components. is there. The illustrated urine collection kit
The urine collection holder 1 is formed in a substantially cylindrical shape, the test tube 2 is inserted into the collection holder 1 when collecting urine, and the bottomed cylindrical urine collection cup 3 is provided. The urine collection tool 4 is attached. Urine collection holder 1
Has a bottomed cylindrical shape having an inner diameter slightly larger than that of the test tube 2, and the operation flange 1 is provided on the outer periphery of the opening 1b.
c is formed. In addition, a thread groove is formed on the inner circumference of the holder front end portion 1a so that the urine collecting tool 4 can be screwed in and attached. The tip of the holder 1a
The thread groove of the urine collecting tool 4 may be omitted, and the urine collecting tool 4 may be fitted to the tip portion 1a of the holder. The urine collection tool 4
It is composed of a urine collecting tool body 5 having an axial through hole, an injection needle 6 communicating with the through hole and fixed to the urine collecting tool body 5, and a needle cover 7 covering the injection needle 6. FIG.
As shown in detail in (b), the urine collection device main body 5 corresponds to a cylindrical urine contact portion 5A, a mounting flange 5B having a diameter larger than that of the urine contact portion 5A, and an inner circumference of the holder tip portion 1a. It is composed of a threaded portion 5C having the above-mentioned thread groove and a tapered portion 5D formed in an arrow cross section. Here, the needle cover 7
Is made of an elastic material, so that the opening 7a of the needle cover is locked to the tapered portion 5D of the urine collection device main body 5 when the injection needle 6 is covered. Further, when the urine collecting tool 4 is screwed into the urine collecting holder 1, the mounting flange 5B is brought into contact with the holder tip portion 1a, and the mounting is completed. Since the ridge 5a extending in the axial direction is provided on the outer circumference of the urine contact portion 5A, the urine collection tool 4 can be easily and reliably mounted on the urine collection holder 1. The test tube 2 is sealed by a cap 2a made of an elastic material, and the inside thereof is maintained in a predetermined reduced pressure state. In addition, an indicator band 2b is provided at an appropriate position on the test tube 2 to clearly indicate the amount of urine to be collected. Since the inside of the test tube 2 is depressurized to a predetermined value in accordance with the amount collected from the urine collection cup 3, the indicator band 2b is usually unnecessary, but the indicator band 2b serves as a mark of the urine collection amount. Even if the cap 2a is accidentally opened, a predetermined amount of urine can be collected. An acid for pH adjustment, an antibacterial agent, a fluorine compound, and an antioxidant are previously enclosed in the test tube 2 in a reduced pressure state, and this is also a feature of the present invention. The pH-adjusting acid adjusts the pH of the collected urine to 4.5-
It is adjusted to 6.5, and granular citric acid is suitable. Therefore, for example, about 4 mg of citric acid is used per 1 ml of urine. As an antibacterial agent, for example, 0.4 to 4.0 mg of EDTA.2Na per 1 ml of urine is used.
As a fluorine compound, for example, 1 per 1 ml of urine
Use more than mg NaF. The same applies to anti-oxidants, although not particularly limited, glutathione is 1 to 5 mg per 1 ml of urine, or ascorbic acid is 1 ml of urine.
1 to 5 mg will be added per time.

Subsequently, a method of using the urine collecting kit shown in FIGS. 1 and 2 will be described. First, the urine of the subject is collected from the bottom of the urine collection cup 3 to about 2 cm. Next, the test tube 2 is inserted into the urine collection holder 1 and stopped before the cap 2a of the test tube 2 hits the needle cover 7 (FIG. 2).
(A)). The work described above can be completed by lightly pressing the test tube 2 with the thumb while the urine collection holder 1 is sandwiched between the index finger and the middle finger, and the two fingers are locked to the operation flange 1c. it can. In this state, the urine contact portion 5A of the urine collecting tool 4 is immersed in the urine of the urine collecting cup 3 and the test tube 2 is further pushed in with the thumb. Then, the injection needle 6 pushes through the needle cover 7 and the cap 2a while the cap 2a and the needle cover 7 are in contact with each other. In response to this operation, the urine contact portion 5A and the test tube 2 are communicated with each other, so that a predetermined amount of urine corresponding to the depressurized state of the test tube 2 is collected in the test tube 2 (see FIG. 3). ). Then, after pulling out the test tube 2 from the urine collection holder 1, the test tube 2 is shaken sufficiently. Since the cap 2a of the test tube is made of an elastic material, the collected urine does not spill out from the hole of the cap 2a after the injection needle 6 is pulled out. Test tube 2
By shaking, the drug in the test tube 2 and the collected urine are mixed, and the denaturation or increase / decrease of the dissolved components in urine can be prevented over a long period of time (JP-A-6-21388).
No. 5). Moreover, since the test tube 2 also contains antioxidants such as glutathione and ascorbic acid,
Even if the collected urine in the test tube 2 is left under high temperature for a long time, the creatinine does not deteriorate. Therefore, the target component concentration should be accurately calculated by using the creatinine as a measurement target. You can An example of the present invention has been described above with reference to FIGS. 1 to 3, but the specific configuration of the urine collection kit is not limited to that shown in the drawings. For example, as shown in FIGS. 4 and 5, the positioning member 1d may be integrally provided on the inner peripheral portion of the urine collection holder 1. In this case, since the positioning member 1d is gradually expanded in correspondence with the insertion of the test tube 2, the positioning tube 1d is not connected to the test tube 2 regardless of the dimensional difference between the inner diameter of the urine collection holder 1 and the outer diameter of the test tube cap 2a. The center of the urine collecting tool 4 can be easily aligned.

[0007]

EXAMPLE FIG. 6 is a drawing showing the effect of adding an antioxidant to a preservative, wherein 4 mg / ml of citric acid was placed in the test tube 2.
, EDTA ・ 2Na 3 mg / ml, NaF 1 mg / ml,
The figure shows the creatinine concentration after urine was collected by enclosing it in an antioxidant of 5 mg / ml and leaving it at 40 ° C. for 96 hours.
7 to 9 show the comparison between the control group and the warming and leaving group (FIG. 7) and the control group and the antioxidant-containing preservative group (FIGS. 8 and 9) for the same example as FIG. It is shown. In this example, ascorbic acid and glutathione were added as antioxidants, but the effect of suppressing the decrease in creatinine concentration is remarkable as compared with the case where no antioxidants were added (warming-treated group). . From the viewpoint of antioxidant ability, it is advantageous to increase the amount of antioxidant added,
When ascorbic acid of 5 mg / ml or more is added, the effect of high creatinine concentration is observed, while when glutathione of 5 mg / ml or more is added, the effect of low creatinine concentration is observed. The addition amount is preferably 1 to 5 mg / ml.
In addition, due to the effect of the preservative, C-peptide, glycine,
Almost no changes were observed in albumin, urea, glucose, etc.

[0008]

As described above, in the invention of claim 1, various acids are added to the collected urine to adjust the pH to 4.5 to 6.5.
Since the antibacterial agent and the fluorine compound are added to the collected urine in addition to the above, it is necessary to prevent denaturation or increase / decrease of dissolved components in urine such as C-peptide, albumin, glycine, urea and glucose over a long period of time. You can Moreover,
In the present invention, since the antioxidant is further added to the collected urine, the concentration of the target component can be accurately detected by using creatinine as a measurement target. In the invention of claim 3,
Since the test tube into which the acid for pH adjustment, the antibacterial agent, the fluorine compound and the antioxidant is put in is maintained in a predetermined depressurized state corresponding to the amount of urine collected, the test tube is injected by the injection needle during urine collection. A predetermined amount of urine can be collected simply by penetrating the cap. Further, the collected urine can be stored in a test tube for a long period of time while preventing the urine dissolved component from being denatured or increased or decreased.

[Brief description of drawings]

FIG. 1 illustrates an example of a urine collecting kit according to the present invention.

FIG. 2 is a diagram illustrating a method of using a urine collecting kit (a) and a shape of a urine collecting tool body.

FIG. 3 is a diagram showing a urine collecting state by a urine collecting kit.

FIG. 4 illustrates another example of the configuration of the urine collection holder.

5 shows the urine collection holder of FIG. 4 in more detail.

FIG. 6 illustrates the effect of adding an antioxidant to a preservative.

FIG. 7 is a diagram comparing a control group and a warming and leaving group.

FIG. 8 is a drawing comparing the control group and the ascorbic acid-added preservative group.

FIG. 9 is a drawing comparing the control group and the glutathione-added preservative group.

[Explanation of symbols]

 1 Urine Collection Holder 1c (For Operation) Flange 2 Test Tube 2a Cap 4 Urine Collection Tool 5 Urine Collection Tool Body 5A Urine Contact Part 6 Injection Needle

Claims (4)

[Claims] 1. When analytically measuring C-peptide, albumin, glycine, urea, glucose, etc., which are dissolved components in urine, various acids are added to the collected urine to adjust the pH to 4.5.
A method for preserving collected urine, characterized in that the antibacterial agent, the fluorine compound, and the antioxidant are added to the collected urine while adjusting to ~ 6.5. 2. The antioxidant is 1 to 5 mg per 1 ml of urine.
The method for preserving collected urine according to claim 1, wherein the amount of glutathione is 1 to 5 mg of ascorbic acid per 1 ml of urine. 3. A tubular urine collection holder having a flange at the base end, a test tube sealed by an elastic cap and inserted into the collection holder from the cap, and a tip of the urine collection holder. A urine collecting kit including a urine collecting tool to be attached to the urine collecting tool, wherein the urine collecting tool is a urine collecting tool body provided with a cylindrical urine contact portion at a distal end thereof, and the urine collecting tool communicating with the urine contact portion. The test tube is provided with an injection needle fixed to the main body, and the test tube corresponds to the amount of collected urine in a state in which an acid for pH adjustment, an antibacterial agent, a fluorine compound, and an antioxidant are added. The urine contact part and the inside of the test tube communicate with each other by allowing the injection needle to penetrate the cap during urine collection. Urine collection kit. 4. The antioxidant is 1 to 5 mg per 1 ml of urine.
The kit for urine collection according to claim 3, which comprises 1 to 5 mg of ascorbic acid per 1 ml of urine.