JPH09191856A - Nutrient-replenishing composition - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain a nutrient-replenishing composition having promotive effect of increasing bone matrix, thus excellent in preventing osteoporosis or preventing aggravation thereof and useful for the aged, etc., blended with calcium and milk-derived basic peptides. SOLUTION: This nutrient-replenishing composition contains (A) 0.1-10wt.% calcium of seaweed or oystershell origin and (B) milk-derived basic peptide as a basic peptide fraction 2000-20000 in molecular weight which is obtained by bringing a milk or milk-derived stock into contact with a cation exchange resin to adsorb basic proteins to the resin followed by eluting the proteins with an elute 0.1-1.0M in salt concentration to obtain a basic protein fraction which is then decomposed by a protease such as pepsin. It is preferable that the weight ratio B/A is 0.1-10.

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to a nutritional supplement composition containing calcium and a milk-derived basic peptide. The composition for nutritional supplementation of the present invention is particularly suitable for application to elderly patients and the like, and a new composition of oral enteral administration for the elderly having an effect of preventing or aggravating osteoporosis associated with lack of exercise during treatment, etc. It is useful as a nutritional supplement composition.

[0002]

2. Description of the Related Art A nutritional supplement composition is prepared by blending natural food-derived food materials and materials obtained by decomposing these to various degrees with oxygen, etc., and has a high calorie and nutritionally balanced composition. Has become. These nutritional supplement compositions are also used when there are diseases such as masticatory disorders and digestive tract disorders, and when long-term nutritional management is required, and various nutritional supplement compositions suitable for such purposes. Is commercially available. And to date, proteins, carbohydrates, lipids, vitamins,
Various compositions for nutritional supplementation have been proposed, in which minerals and the like are used as a calorie source or an essential nutrient source [JP-A 1-157364, JP-A 1-128919, JP-A 2-119762, and JP-A 3 -103154 publication]. However, the subjects using such a nutritional supplement composition are mainly patients after surgery, and their activities are often restricted. It is also well known that proper exercise and nutritional management are important for bone health. However, osteoporosis, which is seen in the elderly and postmenopausal women, has recently become apparent and has become a problem. It is considered that this is due to a decrease in the calcium absorption ability, a decrease in the secretion of active vitamin D ₃ , a hormone imbalance, etc. with the treatment. Therefore, it is not always sufficient to consider calcium and vitamins as in conventional nutritional supplement compositions, and not only calcium but also bone matrix mainly composed of collagen, which is next to calcium in bone, is mainly used. There is a need for a nutritional supplement composition that is considered.

[0003]

DISCLOSURE OF THE INVENTION The present inventors have been searching for a component that increases the bone matrix and a component that suppresses the decrease of the bone matrix contained in food materials. It was found that the milk-derived basic protein fraction that can be obtained by contacting with an ion exchanger has such an action, and that orally ingesting this milk-derived basic protein can improve bone metabolism. I found it [Japanese Patent Application No. 6-261609].

Even if high molecular weight proteins such as lactoferrin and lactoperoxidase contained in the milk-derived basic protein fraction are hydrolyzed in advance by proteolytic enzymes such as pepsin and pancreatin to reduce the molecular weight, The inventors have found that the activity of increasing the bone matrix and the activity of suppressing the decrease of the bone matrix are not lost at all, and have completed the present invention. Therefore, an object of the present invention is to provide a nutritional supplement composition having an excellent nutritional balance, which contains a component that promotes an increase in bone matrix and a component that suppresses a decrease in bone matrix.

[0005]

The nutritional supplement composition of the present invention contains proteins, sugars, lipids, vitamins and minerals as main components, and particularly contains calcium and milk-derived basic peptides. It was done. Then, preferably, a milk-derived basic peptide is added so that the weight ratio is 0.1 to 10 times the amount of calcium in the nutritional supplement composition, and more preferably, the amount of calcium in the nutritional supplement composition is Calcium is added so that it becomes 0.1 to 1.0% by weight.

[0006] As calcium to be added to the nutritional supplement composition of the present invention, calcium obtained from milk or milk-derived raw material [JP-A-6-125740], seaweed-derived calcium,
It is preferable to use calcium derived from natural products such as calcium derived from marine products such as oyster shells, sea urchin shells, squid shells and fish bones, but calcium such as calcium carbonate, calcium gluconate, calcium lactate, calcium citrate and tricalcium phosphate. You may use salt.

The milk-derived basic peptide contained in the nutritional supplement composition of the present invention includes milk-derived basic protein fractions obtained from milk of mammals such as cow milk, human milk, goat milk, and sheep milk. , Preferably, the fraction containing 15% by weight or more of basic amino acids in the amino acid composition is previously hydrolyzed with a proteolytic enzyme such as pepsin or pancreatin, and the molecular weight is adjusted by ultrafiltration membrane treatment, if necessary. A milk-derived basic peptide fraction having a content of 20,000 or less is used. next,
The method for producing the milk-derived basic peptide fraction will be described below.

First, a milk-derived basic protein fraction is prepared. As a method for obtaining a milk-derived basic protein fraction, milk or a milk-derived raw material is brought into contact with a cation exchanger to adsorb the basic protein, and then the basic protein adsorbed on the cation exchanger is adjusted to pH 5 And elution with an eluent having a ionic strength of more than 0.5 [JP-A-HEI 5-
202098], a method using an alginic acid gel [JP-A-61-246198], a method using milk with a sulfated ester [JP-A-63-255300], using inorganic porous particles To obtain from whey [JP-A-1-86839]
Alternatively, a method for producing a bovine insulin-like growth factor-1 containing substance [Japanese Patent Application No. 6-85333] is known.
Then, the milk-derived basic protein fraction obtained by such a method is adjusted to pH 1.5 with a hydrochloric acid solution, hydrolyzed with a proteolytic enzyme such as pepsin, and then alkali solution such as sodium hydroxide. A basic peptide fraction can be obtained by adding and neutralizing. When the milk-derived basic protein fraction is hydrolyzed, it is preferable to add a proteolytic enzyme such as pancreatin to ensure the hydrolysis. The milk-derived basic peptide fraction thus obtained is further desalted and concentrated by a means such as an ion exchange method, a reverse osmosis method, an ultrafiltration method, an electrodialysis method, and if necessary, a freeze-drying method. Or a spray-drying method or the like to dry and blend it into the nutritional supplement composition.

[0009]

BEST MODE FOR CARRYING OUT THE INVENTION The main components of the nutritional supplement composition of the present invention are proteins, sugars, lipids, vitamins and minerals. As the protein, those that are easily digested and have a high nutritional value, such as milk protein, chicken egg protein, soybean protein, or peptides or amino acids which are degradation products of these proteins may be used. As the sugar, dextrin, glucose, fructose, maltose, lactose or the like may be used. As the lipid, it is preferable to use vegetable oil or medium-chain fatty acid triglyceride, and the like.As the vegetable oil, soybean oil containing linoleic acid or α-linolenic acid, rapeseed oil, perilla oil, perilla oil, palm oil, etc. may be used. Good. The medium-chain fatty acid triglyceride is a triglyceride in which a medium-chain fatty acid having 6 to 12 carbon atoms is bonded, and it is known that the medium-chain fatty acid triglyceride has a better digestive absorbability than ordinary fats and oils. And, although it can be changed depending on the purpose of use and usage form, usually about 15 to 40% by weight of protein,
The sugar may be mixed in a ratio of about 50 to 80% by weight and the lipid may be mixed in a ratio of about 10 to 25% by weight. Furthermore, if necessary, as vitamins, vitamins normally used in nutritional supplements, for example,
Vitamin A, Vitamin D, Vitamin E, Vitamin K, Vitamin B ₁ , Vitamin B ₂ , Vitamin B ₆ , Vitamin B
₁₂ , vitamin C, folic acid, niacin, pantothenic acid, choline, inositol, nicotinic acid amide, etc. may be used, and as minerals, sodium, potassium, calcium, magnesium, phosphorus, iron, sulfur, etc. may be used. good.

In the nutritional supplement composition containing the above-mentioned proteins, sugars, lipids, vitamins and minerals as the main components, calcium derived from milk, calcium derived from seaweeds, oyster shells, sea urchins and squids are used as calcium. Shells, calcium derived from natural products such as calcium derived from marine products such as fish bones, calcium carbonate, calcium gluconate, calcium lactate, calcium citrate, tribasic calcium phosphate, etc. as milk-derived basic peptides, milk, human Milk, goat milk, milk-derived basic protein fraction derived from milk of mammals such as sheep milk, preferably a fraction containing 15% by weight or more of basic amino acids in its amino acid composition is pepsin or bread. Preliminarily hydrolyzed with a proteolytic enzyme such as creatine, and if necessary, reduce the molecular weight to 20,000 or less by ultrafiltration membrane treatment. And the milk-derived basic peptide fraction,
Add each. The milk-derived basic peptide may be added to the nutritional supplement composition in a weight ratio of 0.1 to 10 times the amount of calcium contained in the nutritional supplement composition. Calcium may be added so that the amount of calcium contained in the composition is 0.1 to 1.0% by weight.

The nutritional supplement composition of the present invention may be provided in the form of a liquid in which the nutrient components are dissolved or emulsified in water, or may be dried and provided in the form of powder or granules. good. The powdery or granular composition for nutritional supplementation is put into water, preferably 40 to 60 ° C. warm water at the time of oral enteral administration, and is stirred and dissolved before use. In this case, it is preferable to dissolve the aqueous solution so that the calorie of the aqueous solution becomes about 0.5 to 1.5 kcal / ml and about 20 to 25% (w / v). Furthermore, in the case of enteral administration, consider the necessary amount of water and
It is preferable to administer 1,500 to 2,500 ml, and for oral administration, add 400 to 500 ml of the solution to the nutritional supplement composition.
It can also be used in combination with a meal in the form of a beverage prepared by dissolving 90 to 100 g and filling a container such as a sterilized can according to the calorie requirement.

[0012]

[Reference Example 1] After passing 40 liters of unpasteurized cheese whey through a column filled with 400 g of cation exchange resin sulfonated chitopearl (manufactured by Fuji Boseki), this column was washed with deionized water to obtain 0.98 M chloride. The basic protein fraction adsorbed on the resin was eluted with 0.02 M carbonate buffer (pH 7.0) containing sodium. This basic protein fraction was adjusted to pH 1. with a hydrochloric acid solution.
After adjusting to 5, pepsin (manufactured by Kanto Kagaku) was added so as to have a concentration of 2%, and hydrolysis was carried out at 37 ° C. for 1 hour with stirring. Then, after neutralizing to pH 6.8 with sodium hydroxide solution,
Pancreatin (manufactured by Wako Pure Chemical Industries, Ltd.) was added so as to have a concentration of 0.5% and hydrolyzed while stirring at 37 ° C. for 1 hour. Then, the enzyme was inactivated by heating at 85 ° C for 10 minutes. The hydrolyzate of this basic protein fraction was treated with an ultrafiltration membrane with a molecular weight fraction of 20,000, and the permeate was treated with a reverse osmosis membrane for desalting and concentration, and then freeze-dried to give a powdery base. 15 g of the sex peptide fraction was obtained. The component composition of this basic peptide fraction is shown in Table 1, and the composition of the amino acids contained in this basic peptide fraction is shown in Table 2.

[0013]

[Table 1] ───────────────── Moisture 1.0 (wt%) Protein 97.0 Fat 0.4 Ash 0.3 Other 1.3 ─────────────── ──

[0014]

[Table 2] ────────────────── Aspartic acid 11 (wt%) Serine 5 Glutamic acid 12 Proline 6 Alanine 6 Leucine 10 Lysine 9 Histidine 3 Arginine 8 Other 30 ─── ───────────────

[0015]

Test Example 1 With respect to the basic peptide fraction obtained in Reference Example 1, the effect of promoting bone matrix production was examined. That is, the cultured osteoblast-like cell line (MC3T3-E1) was seeded on a 96-well flat-bottom cell culture plate, and cultured for 18 hours in α-MEM medium (manufactured by Flow Laboratories) containing 0.2% by weight of bovine serum (control). group)
. To the test group, 2 μl of a solution prepared by dissolving the basic peptide fraction at 0.5% by weight in 100 μl of the medium was added. Then, after culturing, the medium components were washed with a PBS solution, and the amount of collagen produced by osteoblasts was determined by measuring the amount of hydroxyproline, which is a collagen-specific amino acid. The result is shown in FIG. As is clear from these results, it was confirmed that the test group to which the basic peptide fraction was added produced twice or more collagen as compared with the control group containing only the medium components.

[0016]

Example 1 The basic peptide fraction obtained in Reference Example 1 was blended to prepare a nutritional supplement composition at the blending ratio shown in Table 3. That is, in 60 kg of water, 3.4 kg of milk protein, 1.1 kg of soybean protein and 0.04 kg of basic peptide fraction were dispersed, heated to 70 ° C and dissolved, and then 12.4 kg of dextrin was added to this solution to dissolve. did. Furthermore, 2.4 kg of oils and fats and 0.2 kg of soybean lecithin were added to this solution, preliminarily emulsified with a TK homomixer, then concentrated and spray dried to obtain a powder. Then, appropriate amounts of minerals, vitamins and flavors were added to this powder and mixed to obtain 15 kg of powdered nutritional supplement composition.

[0017]

[Table 3] ────────────────────────────── Milk protein 17.2 (wt%) Soy protein 5.7 Basic peptide fraction 0.2 Oils and fats (soybean oil, palm oil, refined fish oil) 11.9 Soybean lecithin 1.1 Dextrin 62.0 Vitamins 0.1 Minerals 2.8 (calcium 0.2) Perfume Suitable amount ───────────────────── ──────────

[0018]

Test Example 2 The composition powder for nutritional supplementation of the present invention obtained in Example 1 and the control powder produced by the same formulation except that the basic peptide fraction was not added, were used as the bone matrix in rats. The effect on mass was investigated. Ie 27 each
Each powder was dissolved in warm water so that the concentration was g / 100 ml. On the other hand, 6
Six 6-week-old SD female rats were laparotomized under ether anesthesia, and a polyethylene tube with an inner diameter of 0.8 mm was attached to the fundus.
After mounting the gastric catheter, the rat was kept under Bolmann cage restraint. Each of the present invention group and the control group was made up of 3 animals, and each nutritional supplement composition was continuously infused into the stomach with a syringe pump at a rate of 3 ml / hr for 2 weeks. After 2 weeks, the femur of the rat was removed and the amount of amino acid in the bone was measured by a conventional method to be used as an index of the mass of bone base. The result is shown in FIG. From these results, it was confirmed that the amounts of proline and hydroxyproline, which are the constituent amino acids of bone matrix (collagen), were statistically significantly higher in the group of the present invention than in the control group.

[0019]

INDUSTRIAL APPLICABILITY The composition for nutritional supplementation of the present invention has an action of promoting an increase in bone matrix or an action of suppressing a decrease in bone matrix. Therefore, by ingesting the composition, it is possible to prevent osteoporosis and fracture. In addition to nutritional support, it is possible to prevent a decrease in bone mass and bone matrix due to lack of exercise under restraint after surgery. Further, by ingesting it as a nutritional supplement food for post-menopausal women and the elderly, deterioration of osteoporosis can be prevented.

[Brief description of the drawings]

FIG. 1 shows the collagen production promoting action of calcium and milk-derived basic peptide fraction of Test Example 1.

FIG. 2 shows the effect of increasing the femoral matrix of the nutritional supplement composition of the present invention in Test Example 2.

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Claims (5)

[Claims] 1. A nutritional supplement composition comprising calcium and a milk-derived basic peptide. 2. The composition according to claim 1, wherein the milk-derived basic peptide is added in a weight ratio of 0.1 to 10 times the amount of calcium in the composition. 3. The composition according to claim 1, wherein the amount of calcium in the composition is 0.1 to 1.0% by weight. 4. A milk-derived basic peptide is obtained by contacting milk or a milk-derived raw material with a cation exchange resin to adsorb a basic protein, and then eluting it with an eluate having a salt concentration of 0.1 to 1.0M. A basic peptide fraction obtained by degrading a basic protein fraction with a proteolytic enzyme.
4. The composition according to any one of 3. 5. A milk-derived basic peptide is obtained by contacting milk or a milk-derived raw material with a cation exchange resin to adsorb a basic protein and then eluting it with an eluate having a salt concentration of 0.1 to 1.0M. The composition according to any one of claims 1 to 4, which is a basic peptide fraction having a molecular weight of about 2,000 to 20,000 obtained by decomposing a basic fraction with pepsin and further decomposing it with pancreatin.