JPH09169798A - Production of fractionated product consisting mainly of glutenin - Google Patents

Abstract

PROBLEM TO BE SOLVED: To safely obtain at low cost the subject fractionated product in an industrially advantageous way without using any organic solvent by optionally drying extraction residues produced from gluten as a kind of wheat protein by use of an aqueous acidic ethanol solution as extractant. SOLUTION: Gluten as a kind of wheat protein is subjected to extraction for 2h by use of an aqueous acidic ethanol solution prepared by adding 0.01-5.0w/v% of at least one kind of organic acid such as citric acid, lactic acid and/or malic acid to a 1-20vol% aqueous ethanol solution to separate a fractionated product predominant in gliadin, and the resultant extraction residues is obtained as the objective fractionated product consisting mainly of glutenin. Alternatively, the above residues is firtely dispersed in water and then subjected to spray drying to obtain the objective fractionated product. For this fractionated product, there is no need of any explosion-proof apparatus, working safety is high, and there is virtually no effect on its production cost dependent on solvent recovery.

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to a method for producing a fraction containing glutenin as a main component.

[0002]

Glutenin is known as a protein collected from an extraction residue insoluble in an ethanol aqueous solution obtained by separating gliadin extracted from a wheat protein, gluten, by a 70% by volume ethanol aqueous solution. To industrially produce glutenin by this method, the cost of the solvent is high, and the recovery cost thereof is also high. In addition, since the operation is performed in high-concentration ethanol, it is necessary to use a solvent recovery device, an extraction and drying device and its environment as an explosion-proof device, which is a difficult method to employ.

[0003]

SUMMARY OF THE INVENTION It is therefore an object of the present invention to provide a method for producing a fraction containing glutenin as a main component which is inexpensive, safe and easy to industrially employ.

[0004]

Means for Solving the Problems As a result of intensive studies for solving the above problems, the inventors of the present invention have found that even if an aqueous solution having a low ethanol concentration is used, an acidic ethanol aqueous solution does not require an explosion-proof device and the glutenin concentration is easily The inventors have found that it is possible to obtain a high-fractionation product and arrived at the present invention.

That is, the present invention relates to an extract residue obtained by gluten which is a wheat protein by an acidic ethanol water extraction method or a fraction containing glutenin as a main component for drying the extract residue (hereinafter referred to as glutenin main component content). It is sometimes referred to as a painting).

[0006]

BEST MODE FOR CARRYING OUT THE INVENTION The gluten which is a wheat protein used in the present invention may be raw gluten (wet gluten) separated from wheat flour or powdered gluten (vital gluten) which is a dried product thereof.

The method for extracting the glutenin-based fraction according to the present invention is obtained from the residue in gliadin extraction, and utilizes the gliadin extraction residue by acidic ethanol water extraction described in Japanese Patent Application No. 3-49795. Is the way to do it. That is, the acidic ethanol water extraction method in the present invention is performed with 0.01 to 20% by volume aqueous ethanol solution, preferably 5 to 10% by volume aqueous ethanol solution.
5.0 wt / vol% organic acid, preferably 0.2-0.
This is a method in which a solution in which 5% by weight / volume% of organic acid is dissolved is used as an extraction solvent.

Examples of organic acids include citric acid, lactic acid,
One or more selected from malic acid and acetic acid, particularly citric acid is preferably used.

As extraction conditions, powdery gluten 7-1 is used.
Extraction operation is performed for 1 to 3 hours using 0 times the amount of the extraction solvent while keeping the solution temperature at 20 to 30 ° C. in an extraction tank equipped with a propeller or the like. Then, the precipitate, that is, the extraction residue is separated from the supernatant by centrifugation or filtration.
If desired, the separated extraction residue is finely dispersed in water,
Dry it by means such as a flash dryer,
A powder of the glutenin-based fraction is obtained.

The extract residue or dry powder thus obtained contains 45 to 70% by dry weight of glutenin. It is preferably 50% by dry weight or more. Glutenin varies somewhat depending on the type of wheat, but in wheat it is about 4-5.
It is contained in a weight percentage of about 44 to 48 in wheat protein.
It is contained by weight%.

[0011]

The present invention will be described in more detail with reference to the following examples, but the present invention is not limited to these examples.

Example 1 100 g of powdered active gluten was mixed with 10 volumes of 5% by volume ethanol and citric acid acidic aqueous solution (citric acid 0.33% by weight).
Was added and extraction was performed for 2 hours. The residue obtained by separating the extract with a centrifuge was finely dispersed in water and spray-dried to obtain a dry powder 65 of a glutenin-main component fraction. The main components of the fraction were about 50% by weight of glutenin, 25% by weight of carbohydrates, and the other 25% by weight were water, proteins and lipids. In this example, since a solvent having a concentration much lower than 20% by volume, which is the explosion limit of ethanol in a vaporized state, was used, an explosion-proof device was not required, work safety was high, and a cost dependent on the recovery rate of the solvent was used. Has little effect on
The glutenin-based fraction could be separated.

[0013]

As described above, according to the present invention,
Since it is possible to separate from gliadin with a low concentration of ethanol, an explosion-proof device is not required, and a safe and inexpensive industrial production method of a glutenin-based fraction can be provided.

Claims (4)

[Claims] 1. A method for producing an extraction residue obtained by extracting from gluten, which is a wheat protein, by an acidic ethanol water extraction method, or a fraction containing glutenin as a main component for drying the extraction residue. 2. An acidic ethanol water containing 0.01 to 5.0% by weight / volume% of one or more organic acids.
The method for producing a fraction containing glutenin as a main component according to claim 1, which is an aqueous 20% by volume ethanol solution. 3. The method for producing a fraction containing glutenin as a main component according to claim 1, wherein the organic acid is one or more selected from citric acid, lactic acid, malic acid and acetic acid. 4. A fraction containing glutenin as a main component, which is obtained by the production method according to any one of claims 1 to 3.