JPH09154565A - Shaking culture machine - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a shaking culture machine so designed that a culture vessel in a tank subject to temperature control is irradiated with visible light to measure and display the absorbance of microorganisms in a medium to enable the quantity of the microorganisms to be determined without the need of taking out the culture vessel during culture. SOLUTION: A quartz culture vessel 9 to culture microorganisms such as bacteria in a liquid medium 7 is housed in a tank 8 ensured to be subject to temperature control by packing the space between an outer box 2 and an inner box 3 with a thermal insulation material 4, and a platform 10 is vibrated by driving a motor. Subsequently, visible light is emitted from a relevant emitter 12 against the culture vessel 9, and the visible light transmitted through the culture vessel 9 is detected by a transmitted light detector section 13; based on the absorbance obtained, the number of the microorganisms is calculated by a computer 14, and the result is displayed on a display section.

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to a shaking culture machine for culturing microorganisms and the like.

[0002]

2. Description of the Related Art A conventional shaker incubator has a culture vessel such as a test tube, an Erlenmeyer flask, a Sakaguchi flask, etc., containing bacteria to be cultured in a liquid medium inside, fixed to a platform, and the platform is placed in a temperature controllable tank. Shake to the left or right, or rotate or swivel, shake the culture vessel fixed to this platform to uniformly stir the liquid medium in the culture vessel and promote the intake of air into the liquid medium, It was trying to grow microorganisms.

To know the degree of culture of microorganisms in culture,
Remove the culture vessel from the shaking incubator tank each time,
Visible light was directly applied and the absorbance was calculated from the difference from the transmitted light to confirm the number of microorganisms.

[0004]

However, in order to know the number of microorganisms in culture, the culture vessel is taken out from the tank of the shaking incubator at each time, and the visible light is directly applied to determine the absorbance from the difference with the transmitted light. If the quantity of the microorganisms is confirmed by the request, the culture will be suspended, and the culture work will take a long time.

The present invention focuses on the above-mentioned drawbacks of the prior art, and an object thereof is to provide a shaking incubator capable of knowing the number of microorganisms in a shaking incubator without removing the culture container from the tank during the culture. .

[0006]

According to the present invention, in a shaking culture machine in which a culture vessel having a medium for culturing microorganisms such as bacteria is stored in a temperature-controlled vessel, visible light is cultivated in the vessel. It is a constitution provided with means for measuring the absorbance of microorganisms exposed to the sun and displaying the absorbance or a value corresponding to the absorbance.

According to the present invention as set forth in claim 2,
In a shaking culture machine that stores a culture vessel with a medium for culturing microorganisms such as bacteria inside a temperature-controlled vessel, measure the absorbance of the microorganism by applying visible light to the culture vessel in the vessel and In addition, it is configured to have means for displaying the number of microorganisms calculated by the computer.

[0008]

Embodiments of the present invention will be described below.

In FIG. 1, reference numeral 1 designates a shaking culture formed by a main body 5 in which a heat insulating material 4 is filled between an outer box 2 and an inner box 3, and a door 6 which opens and closes an upper opening of the main body 5. It is a machine. Inside the inner box 3 of the main body 5, a tank 8 for maintaining microorganisms such as bacteria in a liquid medium 7 while maintaining a predetermined temperature is formed. The liquid medium 7 is placed in a quartz culture container 9,
It is fixed to a platform 10 provided at the bottom of the tank 8 with a clamp 11, and the motor 9 shakes the platform 10 to agitate the liquid medium in the culture container 9 and promote the intake of air into the liquid medium. , For culturing microorganisms.

In order to know how far the culture is progressing in the process of culturing the microorganism, the visible light emitting unit 12
More visible light is emitted to the culture container 9 containing the liquid medium 7, and the visible light that has passed through the culture container 9 is detected by the transmitted light detection unit 13, and the number of microorganisms is obtained based on the absorptivity of the liquid medium 7. . The absorptance and the number of microorganisms calculated in the computer 14 built in the shaking culture machine 1 based on the absorptance are displayed on the display unit 15 in FIG. This allows
Even during culturing, the number of microorganisms can be known without opening the door 6 and removing the culturing container 9. If the culture container 9 is a glass culture container such as an Erlenmeyer flask, a test tube, or a Sakaguchi flask, the quality and thickness of the glass varies depending on the manufacturer, and the amount of light lost as scattered light tends to vary. Therefore, as shown in FIG. 3, a transparent quartz culture container 9 having a surface perpendicular to the emitted light of visible light is prepared and used to prevent light scattering and to improve the accuracy of counting the number of microorganisms. It is desirable to raise it.

Further, in the above embodiment, as described in claim 2, the visible light is applied to the culture vessel in the tank to measure the absorbance, and the number of the microorganisms calculated by the computer based on the data is displayed. Although it has been described that the means for performing is provided, it is provided with a means for measuring the absorbance of microorganisms by applying visible light to the culture container in the tank as described in claim 1 and displaying the absorbance or a value according to the absorbance. It may be something.

[0012]

As described above, according to the present invention, in a shaking culture machine in which a culture container having a medium for culturing microorganisms such as bacteria is stored in a temperature-controlled tank, visible light is cultivated in the tank. By measuring the absorbance of microorganisms applied to a container and displaying the absorbance or a value corresponding to the absorbance, the number of cultured microorganisms can be known even during culturing without opening the door and removing the culture container.

According to the second aspect of the present invention, the quantity of the microorganisms in the culture is immediately known by calculating and displaying the quantity of the microorganisms by a computer based on the absorbance of the microorganisms in the culture container. be able to.

[Brief description of the drawings]

FIG. 1 is a cross-sectional view of a shaking culture machine of the present invention.

FIG. 2 is a view showing a state where a door of the shaking culture machine of the present invention is opened.

FIG. 3 is a perspective view of a quartz culture container used in the shaking culture machine of the present invention.

[Explanation of symbols]

 1 Shaking Incubator 8 Tank 9 Culture Container 12 Visible Light Emitting Section 14 Computer

Claims (2)

[Claims] 1. In a shaking culture machine in which a culture container having a medium for culturing microorganisms such as bacteria inside is stored in a temperature-controlled tank, visible light is applied to the culture container in the tank to measure the absorbance of the microorganism. A shaking culture machine comprising means for displaying the absorbance or a value corresponding to the absorbance. 2. In a shaking culture machine in which a culture container having a medium for culturing microorganisms such as bacteria inside is stored in a temperature-controlled tank, visible light is applied to the culture container in the tank to measure the absorbance of the microorganism, A shaking culture machine comprising means for displaying the number of microorganisms calculated by a computer based on the data.