CN217237744U

CN217237744U - Data acquisition device for real-time fluorescence quantitative PCR instrument

Info

Publication number: CN217237744U
Application number: CN202220121699.7U
Authority: CN
Inventors: 安可可
Original assignee: Jiangsu Xinlanxin Biotechnology Co ltd
Current assignee: Jiangsu Xinlanxin Biotechnology Co ltd
Priority date: 2022-01-17
Filing date: 2022-01-17
Publication date: 2022-08-19
Anticipated expiration: 2032-01-17

Abstract

A data acquisition device for a real-time fluorescent quantitative PCR instrument comprises a fixed frame, a sealing cover is sleeved at the upper end of the fixed frame, a groove is formed in the inner bottom side of the fixed frame, a driving motor is fixedly connected in the groove, the output end of the driving motor is fixedly connected with a rotating frame, a first bearing is rotatably connected between the fixed frame and the rotating frame, in the process that the driving motor drives the rotating frame to rotate, the stability of the rotating frame in the rotating process is improved by the first bearing, meanwhile, an annular rubber ring and an annular plate are added in a cylinder to fixedly clamp a sample container, and therefore the problem that the data acquisition device for the real-time fluorescent quantitative PCR instrument easily generates shaking in the moving process to cause data acquisition deviation in the current stage can be solved; meanwhile, the photoelectric isolator is arranged on the bottom side of the cylinder and corresponds to the optical element, signal interference is reduced, the temperature inside the fixing frame is controlled by the temperature control block and the temperature sensor, air is drawn by the fan to dissipate heat rapidly, and the accuracy of measured data is improved.

Description

Data acquisition device for real-time fluorescence quantitative PCR instrument

Technical Field

The utility model relates to a fluorescence quantitative PCR appearance technical field, concretely relates to data acquisition device that real-time fluorescence quantitative PCR appearance was used.

Background

The real-time fluorescent quantitative PCR instrument consists of a fluorescent quantitative system and a computer, and utilizes a device for acquiring data and forming a standard curve in a cyclic detection process, so that the concentration of a sample at a position can be calculated by the method.

At present, the data acquisition device for the real-time fluorescence quantitative PCR instrument generally utilizes the optical element to carry out cycle detection on samples with different proportions, but the mode is easily subjected to data acquisition deviation caused by shaking generated in the motion process, and meanwhile, the precision of measured data is reduced due to signal deviation in the acquisition process of the optical unit.

SUMMERY OF THE UTILITY MODEL

The utility model discloses the technical problem who solves: at present, a data acquisition device for a real-time fluorescence quantitative PCR instrument generally utilizes an optical element to perform cycle detection on samples with different proportions, but the mode is easily subjected to data acquisition deviation caused by shaking generated in the motion process, and meanwhile, the problem that the precision of measured data is reduced due to signal deviation in the acquisition process of an optical unit is solved.

The utility model provides a technical scheme that technical problem adopted is: the utility model provides a data acquisition device that real-time fluorescence ration PCR appearance was used, includes fixed frame, the closing cap has been cup jointed to fixed frame upper end, fixed frame inner bottom side is opened and is equipped with recess and recess internal fixedly connected with driving motor, driving motor output fixedly connected with rotates the frame, it is connected with first bearing to rotate between fixed frame and the rotation frame.

As a preferred technical scheme of the utility model, be provided with the fixed plate on circular arch and the arch in the rotation frame, it has spacing knot all to peg graft on four sides on the fixed plate to rotate the frame, protruding upside glue joint in the rotation frame has the rubber pad, convenient fixed and replacement fixed plate.

As a preferred technical scheme of the utility model, fixed plate evenly distributed has drum and drum to be provided with six groups, every group the contained angle is 60 between the drum, it has annular rubber circle to have seted up the interpolation of annular groove and annular groove in the drum, the welding of drum bottom side has the annular slab, makes things convenient for the sampling test.

As a preferred technical scheme of the utility model, closing cap bottom center department fixedly connected with accuse temperature piece, accuse temperature piece electric connection has temperature-sensing ware and temperature-sensing ware model to be PT100, it has circular through-hole to open in the rotation frame, fixed frame both sides are all seted up and are set up the fan in fluted and the recess, can conveniently control the temperature.

As an optimized technical scheme of the utility model, be located accuse temperature piece one side fixedly connected with optical element in the closing cap, optical element corresponds with the drum, rotate frame inside bottom side fixedly connected with photoelectric isolator, the convenient data acquisition.

The utility model has the advantages of it is following: in the process that the rotating frame is driven to rotate by the driving motor, the stability of the rotating frame in the rotating process is improved by the first bearing, and meanwhile, the annular rubber ring and the annular plate are added in the cylinder to fixedly clamp the sample container, so that the problem that the data acquisition device for the real-time fluorescence quantitative PCR instrument is easy to shake in the moving process to cause data acquisition deviation at the present stage can be solved; meanwhile, the photoelectric isolator is arranged on the bottom side of the cylinder and corresponds to the optical element, signal interference is reduced, the temperature inside the fixing frame is controlled by the temperature control block and the temperature sensor, air is drawn by the fan to dissipate heat rapidly, and the accuracy of measured data is improved.

Drawings

FIG. 1 is a schematic cross-sectional side view of a data acquisition device for a real-time fluorescence quantitative PCR apparatus according to a preferred embodiment of the present invention;

fig. 2 is a schematic three-dimensional structure diagram of a data acquisition device for a real-time fluorescence quantitative PCR instrument according to a preferred embodiment of the present invention;

fig. 3 is a schematic top sectional view of a data acquisition device for a real-time fluorescence quantitative PCR instrument according to a preferred embodiment of the present invention.

Description of reference numerals: 1. a fixing frame; 2. sealing the cover; 3. a drive motor; 4. rotating the frame; 5. a first bearing; 6. a fixing plate; 7. a limiting buckle; 8. a cylinder; 9. an annular rubber ring; 10. an annular plate; 11. a temperature control block; 12. a circular through hole; 13. a fan; 14. an optical element; 15. a photoelectric isolator.

Detailed Description

The technical solution of the present invention will be clearly and completely described with reference to the accompanying drawings. In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplification of description, but do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying a relative importance.

In the description of the present invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood in specific cases to those skilled in the art.

The present invention will be further explained with reference to the accompanying drawings.

Please refer to fig. 1-3 in combination with the data acquisition device for a real-time fluorescence quantitative PCR instrument, which comprises a fixed frame 1, which is sealed by the fixed frame 1, the upper end of the fixed frame 1 is sleeved with a sealing cover 2, a groove is arranged on the inner bottom side of the fixed frame 1, and a driving motor 3 is fixedly connected in the groove, the output end of the driving motor 3 is fixedly connected with a rotating frame 4, which is convenient for data acquisition, a first bearing 5 is rotatably connected between the fixed frame 1 and the rotating frame 4, so as to reduce the shake generated in the rotating process of the driving motor 3, and improve the stability of the rotating frame 4 in the rotating process.

Wherein, rubber packing is glued to be connected with the arch, and the arch is located and rotates frame 4, and fixed plate 6 is located the arch, and 6 contacts of fixed plate have limit buckle 7, and limit buckle 7 pegs graft in 6 inner wall four sides of fixed plate, can carry on spacingly to fixed plate 6 through limit buckle 7 and rubber pad, can conveniently replace the different fixed plate 6 of group number distribution simultaneously.

Wherein, the annular plate 10 welds in the 8 bottom sides of drum, and annular rubber circle 9 is pegged graft in the annular groove and the annular groove is located drum 8, and drum 8 is provided with six groups, and every group is provided with a plurality of drums 8, and the contained angle between every group drum 8 is 60, conveniently places the sample of different proportions.

Wherein, fan 13 is located the recess, and the recess is located 1 both sides of fixed frame, and it has filter screen and closing plate to peg graft on the recess, and the closing plate is controlled by accuse temperature piece 11, and accuse temperature piece 11 fixed connection closing cap 2, the temperature-sensing ware model be P100 and temperature-sensing ware electric property response accuse temperature piece 11, and circular through-hole 12 is located and rotates 4 bottom sides of frame, the inside temperature of temperature-sensing ware induction system, opening and shutting of fan 13 in both sides is controlled as required, forms the convulsions structure, dispels the heat fast.

Wherein, optoelectronic isolator 15 fixed connection is in rotating frame 4, and optoelectronic isolator 15 can prevent signal interference, and drum 8 corresponds with optical element 14, and optical element 14 fixed connection is in the inside of accuse temperature piece 11, and driving motor 3 drives and rotates frame 4 and rotate to can make optical element 14 correspond with every group drum 8 many times, thereby pass through optical element 14 data acquisition.

Specifically, the sample is placed in a cylinder 8 according to different proportions, friction is increased through an annular rubber ring 9, the bottom side of the sample is limited by a matching annular plate 10, so that the stability is improved, a sealing cover 2 is covered, a driving motor 3 is started, the driving motor 3 drives a rotating frame 4 to rotate at a constant speed in a fixed frame 1, the fixed frame 1 and the rotating frame 4 are connected through a first bearing 5, the stability of the rotating frame 4 in the rotating process is improved, the internal temperature is sensed through a temperature sensor, temperature adjustment is performed through a temperature control block 11, a partition plate is arranged on the bottom side in the fixed frame 1, a fan 13 is started to adjust to form an air draft structure, so that the purpose of rapid cooling can be achieved, the driving motor 3 is started, the cylinder 8 on the fixed plate 6 rotates, an optical element 14 can collect sample type data on a group of cylinders 8, so as to draw a fluorescence data chart, a photoelectric isolator 15 is arranged on the bottom side of the cylinder 8, can be matched with the optical element 14, reduces signal interference and improves the sensitivity of data collection and system detection.

The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, a plurality of improvements and decorations can be made without departing from the principle of the present invention, and these improvements and decorations should also be regarded as the protection scope of the present invention.

Other parts not described in detail in the present invention belong to the prior art, and are not described herein again.

Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same; although the present invention has been described in detail with reference to the foregoing embodiments, it should be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; such modifications and substitutions do not depart from the spirit and scope of the present invention.

Claims

1. The utility model provides a data acquisition device that real-time fluorescence quantitative PCR appearance was used, includes fixed frame (1), its characterized in that, closing cap (2) have been cup jointed to fixed frame (1) upper end, fixedly connected with driving motor (3) in recess and the recess are opened to fixed frame (1) inner bottom side, driving motor (3) output end fixedly connected with rotates frame (4), fixed frame (1) and rotate and be connected with first bearing (5) between frame (4).

2. The data acquisition device for the real-time fluorescent quantitative PCR instrument as claimed in claim 1, wherein a circular protrusion is arranged in the rotating frame (4) and a fixing plate (6) is arranged on the protrusion, the four sides of the rotating frame (4) on the fixing plate (6) are inserted with limit buckles (7), and the rubber pad is connected to the upper side of the protrusion in the rotating frame (4) by glue.

3. The data acquisition device for the real-time fluorescent quantitative PCR instrument according to claim 2, wherein the fixed plate (6) is uniformly distributed with six groups of cylinders (8), the included angle between each group of cylinders (8) is 60 °, an annular groove is formed in each cylinder (8), an annular rubber ring (9) is inserted in the annular groove, and an annular plate (10) is welded to the bottom side of each cylinder (8).

4. The data acquisition device for the real-time fluorescence quantitative PCR instrument according to claim 1, wherein a temperature control block (11) is fixedly connected to the center of the bottom end of the cover (2), the temperature control block (11) is electrically connected with a temperature sensor of PT100 type, a circular through hole (12) is formed in the inner bottom side of the rotating frame (4), grooves are formed in both sides of the fixing frame (1), and a fan (13) is arranged in each groove.

5. The data acquisition device for the real-time fluorescence quantitative PCR instrument according to claim 4, wherein an optical element (14) is fixedly connected to one side of the temperature control block (11) in the cover (2), the optical element (14) corresponds to the cylinder (8), and a photoelectric isolator (15) is fixedly connected to the inner bottom side of the rotating frame (4).