JPH09151102A - New antibiotic agent - Google Patents
New antibiotic agentInfo
- Publication number
- JPH09151102A JPH09151102A JP33432395A JP33432395A JPH09151102A JP H09151102 A JPH09151102 A JP H09151102A JP 33432395 A JP33432395 A JP 33432395A JP 33432395 A JP33432395 A JP 33432395A JP H09151102 A JPH09151102 A JP H09151102A
- Authority
- JP
- Japan
- Prior art keywords
- antibiotic agent
- gallic acid
- algae
- compound
- gallic
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
Description
【０００１】[0001]
【産業上の利用分野】本発明は、藻類等に対する増殖抑
制作用を有する抗生物剤に関する。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to an antibiotic agent having a growth inhibitory action on algae and the like.
【０００２】[0002]
【従来の技術】藻類（アオコ等）の増殖は水田・湖沼・
芝生等様々な場面で問題となっている。水田では水面に
増殖した藻類により太陽光線が遮られ、水温が上がらな
くなったり、土の表層剥離等により稲の生育を阻害す
る。夏場の湖沼ではアオコ等の大量発生により水道水の
悪臭などの問題が発生する。芝生では藻類の増殖により
芝の生育に影響を及ぼしたり、美観が著しく損なわれる
などの問題が発生している。噴水などでも藻類の増殖に
より美観が損なわれ、その清掃作業に少なからぬ人手と
費用が費やされている。さらに、海洋性の藻類（植物プ
ランクトン等）の増殖、すなわち赤潮は沿岸漁業に毎年
莫大な損害を与えている。2. Description of the Related Art Propagation of algae (blue-green algae etc.)
It is a problem in various situations such as lawns. In the paddy field, the algae that have grown on the surface of the water block the sun's rays, the water temperature does not rise, and the growth of rice is hindered by the surface layer separation of the soil. In lakes in summer, problems such as a bad smell of tap water occur due to the large amount of blue-green algae. On the lawn, there are problems that the growth of algae affects the growth of turf and the aesthetic appearance is significantly impaired. Even in fountains, the growth of algae impairs the aesthetics, and a considerable amount of manpower and money are spent on the cleaning work. Moreover, the growth of marine algae (phytoplankton, etc.), or red tide, causes enormous damage to coastal fisheries every year.
【０００３】しかしながら、藻類の増殖を抑制し、かつ
環境に対する負荷が少ない薬剤は未だ見いだされていな
い。農薬でACN（２−アミノ−３−クロル−１，４−ナ
フトキノン）等が抗藻類剤として実用化されているが、
魚毒性が強く、使用範囲はごく狭いものとなっている。However, a drug that suppresses the growth of algae and has a low environmental load has not yet been found. ACN (2-amino-3-chloro-1,4-naphthoquinone), etc. has been put to practical use as an anti-algal agent as a pesticide.
It is highly toxic to fish and its use range is extremely narrow.
【０００４】[0004]
【発明が解決しようとする課題】本発明は環境に対し負
荷が小さく、また人体に対する毒性の少ない抗生物剤を
提供することを目的とする。SUMMARY OF THE INVENTION It is an object of the present invention to provide an antibiotic agent which has a low load on the environment and is less toxic to the human body.
【０００５】[0005]
【課題を解決するための手段】本発明者らは、上記課題
を解決するために鋭意努力した結果、没食子酸類が藻類
等の生物の増殖抑制に極めて有効であることを見出し、
特許請求の範囲に記載の各請求項の発明により、本課題
を解決することができた。即ち、本発明は、没食子酸類
を有効成分とする抗生物剤である。As a result of intensive efforts to solve the above problems, the present inventors have found that gallic acids are extremely effective in suppressing the growth of organisms such as algae,
The present invention can be solved by the inventions of the claims described in the claims. That is, the present invention is an antibiotic agent containing gallic acid as an active ingredient.
【０００６】本発明に用いる没食子酸類の構造式は下記
の式１に示すものである。The structural formula of gallic acids used in the present invention is shown in the following formula 1.
【０００７】[0007]
【式１】 (Equation 1)
【０００８】例示すれば、没食子酸（Ｒ1、Ｒ2、Ｒ3及
びＲ4が水素原子）、没食子酸ｎ−プロピル（Ｒ1が−Ｃ
Ｈ2ＣＨ2ＣＨ3、Ｒ2、Ｒ3及びＲ4が水素原子）、ｍ−ガ
ロイル没食子酸（タンニン酸ともいう；Ｒ1が式２で表
され、Ｒ2、Ｒ3及びＲ4が水素原子）、For example, gallic acid (R 1 , R 2 , R 3 and R 4 are hydrogen atoms), n-propyl gallate (R 1 is -C)
H 2 CH 2 CH 3 , R 2 , R 3 and R 4 are hydrogen atoms, m-galloyl gallic acid (also referred to as tannic acid; R 1 is represented by Formula 2, and R 2 , R 3 and R 4 are hydrogen). atom),
【式２】 (Equation 2)
【０００９】没食子酸メチル（Ｒ1が−ＣＨ3、Ｒ2、Ｒ3
及びＲ4が水素原子）などが挙げられるが、これらに限
定されるものではない。Methyl gallate (R 1 is --CH 3 , R 2 , R 3
And R 4 are hydrogen atoms), but are not limited thereto.
【００１０】これらの没食子酸類は純品を使用しても良
いが、没食子酸については茶の中に含まれているため、
食品用の茶エキスをそのまま使用しても良い。Although these gallic acids may be pure products, since gallic acid is contained in tea,
Tea extract for food may be used as it is.
【００１１】本発明の抗生物剤は、乳化剤を添加して乳
剤としたり、固体担体に没食子酸類を付着させ粉剤とす
るなど、いかなる形態をとっても良い。乳化剤として
は、各種脂肪酸エステル、例えばグリセリン脂肪酸エス
テル、ショ糖脂肪酸エステル、ソルビタン脂肪エステ
ル、プロピレングリコール脂肪酸エステルが、担体とし
ては例えば、ハイドロキシアパタイト、シリカゲル、ゼ
オライト、炭酸カルシウム、コルク粉末、ベントナイト
等が本発明に使用されるが、その他薬剤学的に許容でき
る乳化剤、担体等も本発明に包含される。また、カプサ
イシン類（特願平7-278220）等の他の抗藻類活性を有す
る物質と組み合わせて用いても良い。The antibiotic agent of the present invention may be in any form such as adding an emulsifier to form an emulsion or attaching a gallic acid to a solid carrier to form a powder. Examples of the emulsifier include various fatty acid esters, such as glycerin fatty acid ester, sucrose fatty acid ester, sorbitan fatty acid ester, and propylene glycol fatty acid ester, and examples of the carrier include hydroxyapatite, silica gel, zeolite, calcium carbonate, cork powder, bentonite, and the like. Although used in the present invention, other pharmaceutically acceptable emulsifiers, carriers and the like are also included in the present invention. It may also be used in combination with other substances having anti-algal activity such as capsaicin (Japanese Patent Application No. 7-278220).
【００１２】[0012]
【実施例】実施例により本発明を具体的に説明するが、
本発明は、これらの実施例に限定されない。EXAMPLES The present invention will be specifically described with reference to examples.
The invention is not limited to these examples.
【００１３】実施例１ 緑藻類に対する増殖抑制効果 緑藻類の被検体としては、国立環境研究所微生物系統保
存施設から分譲された Selenastrum capricornutum NIES
-35を用いた。Ｃ培地（「藻類研究法」共立出版参照）
に3,500 cells/mlの細胞濃度となるように上記細胞を懸
濁し、この懸濁液20mlを細胞培養用フラスコ（Corning
社、カタログ番号430372）に分注した。一方、没食子酸
（和光純薬）、没食子酸n-プロピル（和光純薬）及びタ
ンニン酸（和光純薬）を、それぞれエタノールにて溶解
し、4%w/v（40mg/ml）の溶液を調製して、この溶液を更
に2倍ずつ5段階に希釈した。これらの溶液20μlを、上
記の細胞懸濁液を入れたフラスコに混合した（薬剤の終
濃度は40、20、10、5、2.5、1.25μg/mlである）。対照
としては、エタノール20μlのみを細胞懸濁液を入れた
フラスコに添加したものを用いた。フラスコを、照度5,
000 lx、温度25℃、100rpmの条件で10日間振とう培養し
た後、藻類の増殖量を濁度（A680nm）で測定した。結果
を表１に示す。Example 1 Effect of inhibiting the growth of green algae As a subject of green algae, the National Institute for Environmental Studies microbial strain preservation was used.
Sold from existing facilities Selenastrum capricornutum NIES
-35 was used. C medium (see Kyoritsu Publishing, "Algae Research Method")
Suspend the cells at a cell concentration of 3,500 cells / ml.
20 ml of this suspension becomes cloudy and a cell culture flask (Corning
Company, catalog number 430372). On the other hand, gallic acid
(Wako Pure Chemicals), n-propyl gallate (Wako Pure Chemicals) and Ta
Dissolve phosphonic acid (Wako Pure Chemical Industries) in ethanol.
And prepare a 4% w / v (40 mg / ml) solution, and then add this solution.
It was diluted 2-fold in 5 steps. 20 μl of these solutions,
Mix in the flask containing the cell suspension described above (final drug
The concentrations are 40, 20, 10, 5, 2.5 and 1.25 μg / ml). Contrast
As for the cell suspension, only 20 μl of ethanol was added.
The one added to the flask was used. Light the flask with an illumination of 5,
Shake culture for 10 days at 000 lx, temperature 25 ° C, 100 rpm.
After that, the growth amount of algae was measured by turbidity (A680nm). result
Are shown in Table 1.
【００１４】[0014]
【表１】 [Table 1]
【００１５】実施例２ 藍藻類に対する増殖抑制効果 藍藻類の被検体としては、国立環境研究所微生物系統保
存施設から分譲された Microcystis aeruginosa f. aeru
ginosa NIES-44を用いた。ＣＢ培地（「藻類研究法」共
立出版参照）に、35,000 cells/mlの細胞濃度となるよ
うに上記細胞を懸濁し、この懸濁液20mlを細胞培養用フ
ラスコ（Corning社、カタログ番号430372）に分注し
た。没食子酸n-プロピル（和光純薬）をエタノールにて
溶解し、4%w/v（40mg/ml）の溶液を調製して、更に2倍
ずつ12段階に希釈した。本溶液20μlをフラスコに混合
した（薬剤の終濃度は、40、20、10、5、2.5、1.25、0.
63、0.31、0.16、0.08、0.04、0.02、0.01μg/ml）。対
照としては、エタノール20μlのみを細胞懸濁液を入れ
たフラスコに添加したものを用いた。フラスコを、照度
1,500 lx、温度25℃の条件で10日間静置培養した後、藻
類の増殖量を濁度（A680nm）で測定した。結果を表２に
示す。Example 2 Inhibitory effect on growth of cyanobacteria As a test subject of cyanobacteria, the National Institute for Environmental Studies microorganism strain preservation was used.
Sold from existing facilities Microcystis aeruginosa f.aeru
ginosa NIES-44 was used. CB medium (with "algal research method")
The cell concentration will be 35,000 cells / ml.
Suspension of the above cells and 20 ml of this suspension for cell culture.
Dispense into Rusco (Corning, Catalog No. 430372)
Was. N-Propyl gallate (Wako Pure Chemical Industries) in ethanol
Dissolve and prepare a 4% w / v (40 mg / ml) solution, and double the volume.
Each was diluted in 12 steps. Mix 20 μl of this solution into the flask
(The final concentration of the drug was 40, 20, 10, 5, 2.5, 1.25, 0.
63, 0.31, 0.16, 0.08, 0.04, 0.02, 0.01 μg / ml). versus
As a reference, put only 20 μl of ethanol into the cell suspension.
The one added to the flask was used. Illuminate the flask
After static culture for 10 days at 1,500 lx and temperature of 25 ℃,
The growth amount of the species was measured by turbidity (A680nm). The results are shown in Table 2.
Show.
【００１６】[0016]
【表２】 [Table 2]
【００１７】[0017]
【発明の効果】本発明により、人体に対して安全であ
り、かつ環境に対する負荷が少ない没食子酸類を有効成
分とする抗生物剤を市場に供給することが可能となる。Industrial Applicability According to the present invention, it is possible to supply to the market an antibiotic agent containing gallic acid as an active ingredient, which is safe for the human body and has a low environmental load.
───────────────────────────────────────────────────── フロントページの続き (72)発明者 小田 宗宏 神奈川県小田原市成田540番地 明治乳業 株式会社細胞工学センター内 (72)発明者 鈴木 匡之 神奈川県小田原市成田540番地 明治乳業 株式会社細胞工学センター内 (72)発明者 佐野 晴彦 神奈川県小田原市成田540番地 明治乳業 株式会社細胞工学センター内 ─────────────────────────────────────────────────── ─── Continuation of the front page (72) Inventor Munehiro Oda 540 Narita, Odawara-shi, Kanagawa Meiji Dairy Products Co., Ltd., Cell Engineering Center (72) Inventor Masayuki Suzuki, 540 Narita, Odawara, Kanagawa Meiji Dairy Products Co., Ltd. (72) Inventor Haruhiko Sano 540 Narita, Odawara-shi, Kanagawa Meiji Dairy Co., Ltd., Cell Engineering Center
Claims (4)
プロピル及びｍ−ガロイル没食子酸からなる群から選ば
れたものである請求項１に記載の抗生物剤。2. The gallic acids are gallic acid and gallic acid n-.
The antibiotic agent according to claim 1, which is selected from the group consisting of propyl and m-galloyl gallic acid.
む請求項１又は請求項２に記載の抗生物剤。3. The antibiotic agent according to claim 1 or 2, which comprises a pharmaceutically acceptable carrier or emulsifier.
請求項３に記載の抗生物剤。4. The antibiotic agent according to claim 1, 2, or 3, which is an anti-algal agent.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP33432395A JPH09151102A (en) | 1995-11-30 | 1995-11-30 | New antibiotic agent |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP33432395A JPH09151102A (en) | 1995-11-30 | 1995-11-30 | New antibiotic agent |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH09151102A true JPH09151102A (en) | 1997-06-10 |
Family
ID=18276077
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP33432395A Pending JPH09151102A (en) | 1995-11-30 | 1995-11-30 | New antibiotic agent |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH09151102A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2018166973A1 (en) * | 2017-03-15 | 2018-09-20 | Biocap Tech | Algicidal, algistatic, herbicidal and herbistatic treatment of water |
-
1995
- 1995-11-30 JP JP33432395A patent/JPH09151102A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2018166973A1 (en) * | 2017-03-15 | 2018-09-20 | Biocap Tech | Algicidal, algistatic, herbicidal and herbistatic treatment of water |
| FR3063871A1 (en) * | 2017-03-15 | 2018-09-21 | Biocaptech | ALGICIDE, ALGISTATIC, HERBICIDE AND HERBISTATIC TREATMENT OF WATER |
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