JPH09100202A - Anti-organism agent - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain an anti-organism agent having inhibitory effect on proliferation of various organisms such as algae causing troubles in many scenes such as paddy field, lake and marsh, lawn, etc., comprising a specific compound contained in essence of Guinea pepper for an inexpensive food as an active ingredient. SOLUTION: This anti-organism agent comprises a capsaicin of the formula (R is a 7-12C alkyl or alkenyl). Capsaicin [R is (CH2 )4 CH=CHCH(CH3 )2 ], dihydrocapsaicin, norcapsaicin, homocapsaicin, octanoic acid vanillylamide, etc., may be cited as the capsaicin. The anti-organism agent can be made into a cream by adding an emulsifying agent or into a dust by attaching the capsaicin to a solid carrier. The anti-organism agent has low load on environment and low toxicity to human body.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

TECHNICAL FIELD The present invention relates to a growth inhibitor for various organisms such as algae.

[0002]

2. Description of the Related Art Propagation of algae has become a problem in various fields such as paddy fields, lakes, and lawns. In the paddy field, the algae that grow on the surface of the water block the sun's rays, which prevents the water temperature from rising and causes the surface layer of the soil to peel off. In lakes in summer, problems such as bad smell of tap water occur due to the large amount of blue-green algae. On the lawn, there are problems such as aesthetic deterioration due to the growth of algae. Even in fountains, the growth of algae impairs the aesthetics, and a considerable amount of manpower and money are spent on the cleaning work. Moreover, the growth of marine algae (phytoplankton), or red tide, causes immense damage to coastal fisheries every year.

However, a drug that suppresses the growth of algae and has a low environmental load has not yet been found. ACN (2-amino-3-chloro-1,4-naphthoquinone), etc. has been put to practical use as an anti-algal agent as a pesticide.
It is highly toxic to fish and its use range is extremely narrow.

[0004]

SUMMARY OF THE INVENTION It is an object of the present invention to provide an antibiotic agent which has a low load on the environment and is less toxic to the human body.

[0005]

Means for Solving the Problems As a result of diligent efforts to solve the above problems, the present inventors have found that capsaicins are extremely effective in suppressing the growth of organisms such as algae, and solved the present invention. . The present invention can be solved by the inventions of the claims described in the claims. That is,
The present invention is an antibiotic agent containing capsaicins as an active ingredient.

The structural formula of the capsaicins used in the present invention is shown in the following formula 1.

[0007]

(Equation 1)

For example, capsaicin [R is (CH ₂ ) ₄ CH =
CHCH (CH ₃ ) ₂ ], dihydrocapsaicin [R is (CH ₂ ) ₆ CH (CH
₃ ) ₂ ], norcapsaicin [R is (CH ₂ ) ₃ CH = CHCH (C
H ₃ ) ₂ ], homocapsaicin [R is (CH ₂ ) ₅ CH = CHCH (C
H ₃ ) ₂ ], vanillyl octanoate [R is (CH ₂ ) ₆ CH ₃ ],
Nonanoic acid vanillyl amide [R is (CH ₂ ) ₇ CH ₃ ] and the like can be mentioned, but the invention is not limited thereto.

Capsaicins may be pure products, but cheaper capsicum extract for food may be used.

The antibiotic agent may be in any form such as adding an emulsifier to form an emulsion, or capsaicin to a solid carrier to form a powder. As the emulsifier, various fatty acid esters, for example, glycerin fatty acid ester,
Sucrose fatty acid ester, sorbitan fatty acid ester, propylene glycol fatty acid ester are used as the carrier in the present invention, for example, hydroxyapatite, silica gel, zeolite, calcium carbonate, cork powder, bentonite, etc. Possible emulsifiers, carriers and the like are also included in the present invention. Further, as shown in Tables 2 and 3, when capsaicins are held in a carrier or an emulsifier, the effect of suppressing the growth of algae is enhanced.

[0011]

EXAMPLES The present invention will be specifically described with reference to examples.
The invention is not limited to these examples.

Example 1 Inhibition of Algae ( Oocystis sp.) Growth Oacystis sp. Of the test algae was isolated from a domestic aquarium and identified. Capsaicin (Sigma) and dihydrocapsaicin (Sigma) were each dissolved in ethanol to prepare a 1% (10 mg / ml) solution. 10 μl of this solution
6-well plastic plate for cell culture (Costar 359
It was dripped on the bottom of 6) and air dried. To each well, 10 ml of Oocystis sp. Cell suspension prepared at a cell concentration of 3,500 cells / ml in C medium (see “Algae Research Method” Kyoritsu Shuppan) was dispensed (final concentration of drug was 10 μg / ml). As a control, a drug-free system was set up. After incubating for 7 days under the conditions of illuminance of 5,000 lx and temperature of 25 ° C, the growth amount of Oocystis sp.
nm). Table 1 shows the results.

[0013]

[Table 1]

Example 2 Anti-algal activity of capsaicin adsorbed on various solid supports Capsaicin (Sigma) was dissolved in ethanol, and silica gel (Wako Pure Chemical), hydroxyapatite (Wako Pure Chemical), zeolite (Wako Pure Chemical) ), Calcium carbonate (Wako Pure Chemical Industries, Ltd.), 1% (w / w) on various solid carriers such as cork powder (φ1mm)
Was adsorbed at a weight ratio of. As a spreading agent, 5% (w / w) of glycerin fatty acid ester (RIKEN vitamin) as a food additive was used. 200 ml of plastic for cell culture
100 mg was added to a bottle (Falcon 3110). 50 ml of the Oocystis sp. Cell suspension prepared in the same manner as in Example 1 was dispensed into a bottle and shake-cultured under light irradiation under the culture conditions of Example 1 (capsaicin final concentration 20 μg / ml). As a control, a system in which capsaicin dissolved in ethanol was spread on the bottom surface of the bottle to a final concentration of 20 μg / ml was placed. The amount of growth was collected by centrifuging algal cells, extracting chlorophyll with acetone,
It measured quantitatively with the spectrophotometer. Table 2 shows the results.

[0015]

[Table 2]

Example 3 Anti-algal activity of capsaicin emulsifier Capsaicin (Sigma) was emulsified with glycerin fatty acid ester (RIKEN vitamin) as a food additive to obtain a capsaicin emulsion. The concentration of capsaicin in the emulsion is 2% (w
/ w) and the fatty acid ester was 5% (w / w). These drugs were added to plastic 200 ml bottles for cell culture (Falcon 311
0, 25, 50 and 100 μl were added to Oocys under the same conditions as in Example 2.
The tis sp. was cultured (capsaicin final concentration 20 μg / ml).
20 μg of capsaicin dissolved in ethanol as a control
The spread system was placed on the bottom of the bottle to a final concentration of ml. The amount of proliferation was measured as in Example 2. Table 3 shows the results
Shown in

[0017]

[Table 3]

[0018]

INDUSTRIAL APPLICABILITY According to the present invention, it is possible to supply to the market an antibiotic agent containing capsaicins as an active ingredient, which is safe for the human body and has a low environmental load.

 ─────────────────────────────────────────────────── ─── Continuation of front page (72) Inventor Sadatoshi Sakuma 540 Narita, Odawara-shi, Kanagawa Meiji Dairy Co., Ltd. Cell Engineering Center

Claims (3)

[Claims] 1. An antibiotic agent containing capsaicin as an active ingredient. 2. The antibiotic agent according to claim 1, which comprises a pharmaceutically acceptable carrier or emulsifier. 3. The antibiotic agent according to claim 1, which is an anti-algal agent.