JPH09145712A - Analyser - Google Patents

Abstract

PROBLEM TO BE SOLVED: To accurately, inexpensively, and easily check the completion of measurement using an analyser utilizing immunity chromatography with a non-aqueous colored material. SOLUTION: A non-aqueous colored material 4 is immobilized at the downstream side of a measurement region 2 due to antigen/antibody reaction in a chromatography material 1. A cover member 3 for transmitting light due to the adhesion of a solution is provided so that the upper surface of the non- aqueous colored material 4 can be covered. When the solution is added and reaches the measurement region 2, the solution is analyzed by the antigen/ antibody reaction with an object to be analyzed. When the solution advances to a part where the non-aqueous colored material 4 is immobilized, the cover member 3 transmits light due to the adhesion of the solution, thus indicating that the measurement is completed.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to an analyzer using immunochromatography, and in particular, to improve it so as to more surely confirm that the measurement has been completed.

[0002]

2. Description of the Related Art Conventionally, as an analyzer using immunochromatography, for example, anyone who can easily detect the presence of pregnancy by detecting a specific hormone secreted in urine, etc. There is.

In such an analyzer, it is used that the urine attached to one end of the chromatographic material has moved through the chromatographic material and has passed through the measurement region for detecting a specific hormone, and the measurement has been completed. In order to inform the person, there has been conventionally used a gold colloid and a latex labeled mouse antibody as a labeled substance.

This is because when the anti-mouse antibody is immobilized on the chromatographic material on the downstream side of the measurement region, coloring by the above-mentioned label is observed at the portion where the anti-mouse antibody is immobilized by the antigen-antibody reaction. By this, the user confirms the end of the measurement.

[0005]

However, in the analyzer in which the above-mentioned conventional antigen-antibody reaction is utilized to confirm the end of measurement, since the antigen and the antibody are proteins, the influence of temperature, humidity, etc. There is a problem in that the color strength is unstable and the color strength when colored is unstable. Moreover, the cost of the antibody was very high.

[0006]

In order to solve this problem, the present invention provides an analyzer using immunochromatography in which a water-insoluble colored material is immobilized on a chromatographic material, and The upper surface is covered with a cover member that transmits light when a solution is attached.

As a result, when the solution has passed through the measurement area and the measurement has been completed, light can be transmitted through the cover member and the color of the water-insoluble colored material can be confirmed. Regardless of the influence, it is possible to surely inform the user with a sign of a certain darkness.

[0008]

BEST MODE FOR CARRYING OUT THE INVENTION The invention according to claim 1 of the present invention is characterized in that a solution applied to one end of a chromatographic material passes through a measurement region for detecting an analyte contained in the solution. In order to make it possible to detect the end of the measurement, a water-insoluble colored material is immobilized on the chromatography material at a position downstream of the measurement region, and the water-insoluble colored material is further immobilized. In the analysis device, the upper surface of the chromatographic material is coated with a cover member that transmits light when the solution adheres to the chromatographic material. The water-insoluble colored material fixed in a fixed shape cannot be confirmed because it is covered with the cover member until the solution arrives, but the impermeable member becomes transparent as the solution arrives. By changing the sex, so that color can be confirmed clearly.

The invention according to claim 2 of the present invention resides in the analyzer according to claim 1, characterized in that a water-insoluble colored material is fixed to the cover member. The color of the water-soluble colored material can be confirmed more clearly.

According to a third aspect of the present invention, the solution applied to one end of the chromatographic material passes through a measurement region for detecting an analyte contained in the solution, and the measurement is performed. In order to be able to detect that it has finished,
The chromatography material is composed of a member that transmits light when the solution is attached,
At the position on the downstream side of the measurement region, there is an analyzer characterized by immobilizing a water-insoluble colored material on the chromatography material, since a cover member is unnecessary,
It can be constructed at a lower cost.

According to a fourth aspect of the present invention, the solution applied to one end of the chromatographic material passes through a measurement region for detecting an analyte contained in the solution, and the measurement is performed. In order to be able to detect that it has finished,
At a position on the downstream side of the measurement region, a water-insoluble colored material is immobilized on the chromatography material, and the water-soluble and light-transmitting light is transmitted through the upper surface of the chromatography material on which the water-insoluble colored material is immobilized. In the analyzer, which is characterized in that the immobilized colored material is exposed by the elution of the substance, which is immobilized in a predetermined shape at a predetermined location of the chromatography material. It cannot be confirmed that the water-insoluble colored material is covered with the water-soluble impermeable material until the solution arrives, but the water-soluble impermeable material elutes as the solution arrives, and the color of the water-insoluble colored material is changed. You will be able to see clearly.

(Embodiment 1) An embodiment of the invention described in claim 1 of the present invention will be described below with reference to FIG. In the analyzer shown in FIG. 1, 1 indicates a chromatography material, which is made of glass fiber filter paper. 2
Indicates the measurement area of the antigen utilizing the antigen-antibody reaction on the chromatographic material, and the antibody immobilized on the glass fiber filter paper and the colloidal gold-labeled antibody are sandwiched by antibodies having different epitopes. Take a measurement.

Reference numeral 4 is a water-insoluble colored material fixed on a chromatography material, and an oily red ink is used for this. Reference numeral 3 denotes a cover member provided so as to cover the water-insoluble colored material 4, which is made of a material that transmits light when a solution is attached, such as nitrocellulose.

When the solution is added to one end of the chromatographic material 1, the solution moves through the chromatographic material 1 and the analyte in the measurement region 2 undergoes an antigen-antibody reaction. Then, the solution further proceeds on the chromatography material 1 to the downstream side, and reaches the portion where the water-insoluble colored material 4 is immobilized. Along with this, the solution adheres to the cover member 3
Water-soluble colored material 4 because it changes to transmit light.
Can be recognized from the outside and the end of measurement can be confirmed.

The member that becomes permeable by adhering the solution is
Any material such as a hygroscopic material, a porous material, a fibrous material, or a moisture impermeable material can be used as long as it is a material whose permeability changes due to the attachment of a solution.

(Embodiment 2) An embodiment of the invention described in claim 2 of the present invention will be described below with reference to FIG. In the analyzer of FIG. 2, 5 indicates a chromatography material, which is composed of glass fiber filter paper.
Reference numeral 6 denotes an antigen measurement region using the antigen-antibody reaction, and the antibody immobilized on the glass fiber filter paper and the gold colloid-labeled antibody are subjected to a sandwich reaction with an antibody having a different epitope to measure the antigen. . Further, a glass fiber filter paper was used for the cover member 7 installed on the chromatography material 5 on the downstream side of the measurement region 6 and adapted to transmit light due to the attachment of the solution. The water-insoluble colored material 8 is fixed to the cover member 7. As the water-insoluble colored material 8, oil-based red ink was used.

Before the solution reaches, the water-insoluble colored member 8 cannot be seen from the upper part of the cover member 7. When the solution is added, the solution proceeds with the chromatographic material, and the analyte in the reaction region 6 undergoes an antigen-antibody reaction. Then, the solution further advances the chromatography material 5 and reaches the portion where the water-insoluble colored material 8 is immobilized. At the same time, since the cover member 7 becomes transparent to the light due to the adhesion of the solution, the water-insoluble colored material 8 can be recognized from above and the end of the measurement can be confirmed.

In the above description, the glass fiber filter paper is used as the member that becomes permeable when the solution is attached, but a hygroscopic material can be used as long as it is a material that becomes permeable when the solution is attached. It may be composed of any material such as a porous material, a fiber material, and a moisture impermeable material.

(Embodiment 3) An embodiment of the invention described in claim 3 of the present invention will be described below with reference to FIG. In the analyzer shown in FIG. 2, 9 indicates a chromatographic material, which is composed of nitrocellulose. The chromatographic material 9 normally does not transmit light, but when the solution is attached, it transmits light. Reference numeral 10 denotes an antigen measurement region utilizing an antigen-antibody reaction, and the antibody immobilized on the chromatographic material 9 and the colloidal gold-labeled antibody are measured by a sandwich reaction with an antibody having a different epitope. I do. Further, the water-insoluble colored material 11 fixed on the downstream side of the measurement region 10 uses the oily red ink and cannot be recognized from above before the solution reaches it.

As the added solution moves, the analyte in the measurement region 10 first undergoes an antigen-antibody reaction. After that, the solution further advances on the chromatography material 9 and reaches the portion where the water-insoluble colored material 11 is fixed. At the same time, the adhesion of the solution causes the chromatographic material 9 to transmit light, so that the water-insoluble colored material 11 can be recognized from above and the end of measurement can be confirmed. When the chromatographic material is lined with an impermeable material, the water-insoluble colored material can be more clearly identified.

In the above description, the example in which the chromatographic material is made of nitrocellulose has been described, but other chromatographic materials that become permeable by the attachment of a solution can be similarly applied.

(Embodiment 4) An embodiment of the invention described in claim 4 of the present invention will be described below with reference to FIG. In the analyzer of FIG. 4, reference numeral 12 denotes a chromatography material, which is composed of glass fiber filter paper. Reference numeral 13 denotes an antigen measurement region utilizing an antigen-antibody reaction, and the antigen is measured by a sandwich reaction between an antibody immobilized on a glass fiber filter paper and an antibody having an epitope different from the gold colloid-labeled antibody. Further, the water-insoluble colored material 15 immobilized on the downstream side of the measurement region 13 is
Oily red ink is used. Sodium chloride is provided as the water-soluble impermeable substance 14 so as to cover the water-insoluble colored material 15.

When the solution is added, the chromatographic material 12 advances and the analyte in the reaction region 13 undergoes an antigen-antibody reaction. After that, the solution further proceeds on the chromatography material and reaches the portion where the water-insoluble colored material 15 is immobilized. At the same time, the water-soluble impermeable substance 14 elutes and the water-insoluble colored material 15 is exposed, so that it can be recognized from the outside and the end of the measurement can be confirmed. Here, as the water-soluble impermeable substance, sodium chloride was described as an example,
Other than the above, the invention can be carried out in the same manner even if it is composed of water-soluble impermeable substances such as inorganic salts and organic salts.

In the above description, an example in which the water-insoluble colored material is composed of oily red ink has been described.
The same can be applied to a water-insoluble colored tape, a water-insoluble colored ink, and a water-insoluble dye.

Further, in the above description, an example in which glass fiber filter paper is used as the chromatography material has been described, but the chromatography material may be selected depending on the selection.
The same can be applied to cellulose, cellulose derivatives, porous materials such as filter paper, and fibrous materials.

[0026]

As described above, according to the present invention, the advantageous effect that the end of the measurement in immunochromatography can be easily confirmed with high accuracy, low cost and with the water-insoluble colored material is obtained.

[Brief description of the drawings]

FIG. 1 is an exploded perspective view showing an analyzer according to an embodiment of the present invention.

FIG. 2 is an exploded perspective view showing an analyzer according to an embodiment of the present invention.

FIG. 3 is a perspective view showing an analyzer according to an embodiment of the present invention.

FIG. 4 is an exploded perspective view showing an analyzer according to an embodiment of the present invention.

[Explanation of symbols]

 1,5,9,12 Chromatographic material 2,6,10,13 Measurement area 3,7 Cover member 4,8,11,15 Water-insoluble colored material 15 Water-insoluble colored material

Claims (4)

[Claims] 1. A method for allowing a solution applied to one end of a chromatographic material to pass through a measurement region for detecting an analyte contained in the solution and detecting the end of the measurement. , At a position downstream of the measurement region, a water-insoluble colored material is immobilized on the chromatography material, and the upper surface of the chromatography material on which the water-insoluble colored material is immobilized further adheres to the solution. An analyzer characterized by being covered with a cover member that allows light to pass through. 2. The analyzer according to claim 1, wherein the water-insoluble colored material is fixed to the cover member. 3. A solution applied to one end of a chromatographic material is passed through a measurement region for detecting an analyte contained in the solution, so that the end of the measurement can be detected. The chromatography material is composed of a member that transmits light when the solution adheres, and a water-insoluble colored material is immobilized on the chromatography material at a position on the downstream side of the measurement region. An analyzer characterized by the above. 4. A solution applied to one end of a chromatographic material is passed through a measurement region for detecting an analyte contained in the solution, so that the end of the measurement can be detected. , At a position on the downstream side of the measurement region, a water-insoluble colored material is immobilized on the chromatography material, and the upper surface of the chromatography material on which the water-insoluble colored material is further immobilized is water-soluble and light-exposed. An analyzer, characterized in that it is covered with a substance that does not permeate, and the immobilized colored material is exposed by elution of the substance.