JPH09140673A - Spectra measuring apparatus and method for measuring spectra - Google Patents

Abstract

PROBLEM TO BE SOLVED: To improve the operationability by automating a processing accompanied with a biotic reaction measurement on smooth muscle, etc., and by providing a constitution in which a biotic reaction responding to a stimulation is converted to a time series digital signal and the converted digital signal is converted to periodical spectra. SOLUTION: Previously, a power spectra measuring apparatus 10 is started up and calibration is carried out. First, a pressure as a standard is loaded to a pressure transducer 12 and the output from the pressure transducer 12 is adjusted so that a voltage input to a RAM 38 through a pressure transducer I/F 34 should have a given voltage. The pressure transducer 12 is inserted into a uterus as the tissue of smooth muscle filled with water. Next, an A/D converted inner pressure data converted by the pressure transducer I/F 34 is sent through an amplifier 14 and it is displayed as a graph showing the relation between time and voltage on a CRT 28, a power value of the spectra for every frequency components can be obtained from the inner pressure data and the spectra are presented on the CRT 28.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a spectrum measuring apparatus and a spectrum measuring method for converting a time-series digital signal converted from a biological response to a stimulus into a periodic spectrum.

[0002]

2. Description of the Related Art In order to investigate the response of smooth muscle to a stimulus such as medication, a biological response measuring device which detects the maximum value of the contraction and relaxation of the smooth muscle and records the contraction and relaxation of the smooth muscle on paper has been conventionally used. Was used from.

[0003]

[Problems to be Solved by the Invention] However, depending on the medication,
When the maximum response of contraction or relaxation of smooth muscle is elicited,
There are cases where the frequency of smooth muscle contraction increases or decreases, and cases where the two are combined. When the frequency of smooth muscle contraction increases or decreases, or the maximum response of contraction or relaxation is induced. When the frequency increased or decreased, the measurer had to read the maximum value of contraction and relaxation and the number of peaks of contraction or relaxation per unit time from the data recorded on the paper.

The present invention has been made in consideration of the above facts, and it is an object of the present invention to automate the process involved in the measurement of biological reaction of smooth muscle and improve the operability.

[0005]

According to the present invention, the digital converting means converts a biological response to a stimulus into a time-series digital signal. Further, the spectrum converting means converts the converted digital signal into a periodic spectrum.

As a result, the biological response to the stimulus is automatically converted into a periodic spectrum, and the operability of measuring the biological response is improved. Moreover, the effect of the stimulus can be determined based on the periodic spectrum of the specific range.

[0007]

DESCRIPTION OF THE PREFERRED EMBODIMENTS FIG. 1 shows an outline of a power spectrum measuring apparatus 10 according to an embodiment of the present invention. The power spectrum measuring apparatus 10 includes a pressure transducer 12 that converts pressure into an electric signal, and an amplifier 14 that amplifies the electric signal from the pressure transducer 12.
A strain gauge 16 for converting strain into an electric signal, an amplifier 18 for amplifying the electric signal from the strain gauge 16, and a data processing unit 20 for processing the electric signal inputted from the amplifier 14 and the amplifier 16. There is.

Further, the data processing unit 20 is the data processing unit 2
0, a CPU 22 for controlling the entire data processing unit, a ROM 24 for storing a program for controlling the entire data processing unit 20 and a control program described later, a keyboard 26 for inputting necessary data, and a CRT 28 for displaying a graph or the like.
A printer 30 for printing a graph displayed on the CRT 28, a disk drive 32 for driving a disk such as a floppy disk, a hard disk and a magneto-optical disk as an external memory, and an amplifier 14, and An electric signal from the amplifier 14 is sampled at a sampling cycle of 0.1 kHz to several kHz and converted into internal pressure data which is time-series digital data A
A which is connected to the pressure transducer I / F (interface) 34 having a / D (analog-digital) converter and the amplifier 18 and which samples the electric signal from the amplifier 18 at a sampling period of 0.1 kHz to several kHz. Strain gauge I / F 36 having a / D converter, and a keyboard 26, a pressure transducer I / F 34 and a strain gauge I / F 36 used as a work area.
And a RAM 38 in which input data from is stored, which are interconnected by a bus 40.

Next, the power spectrum measuring apparatus 10
The operation of the CPU 22 will be described. When measuring the power spectrum, the power spectrum measuring apparatus 10 is turned on in advance to start up the power spectrum measuring apparatus 10.

In step 100 of FIG. 2, calibration is performed. In this calibration, the reference pressure is applied to the pressure transducer 12, and the output of the pressure transducer 12 is adjusted so that the voltage value input to the RAM 38 via the pressure transducer I / F 34 becomes a predetermined voltage value. To do.

After the calibration, the pressure transducer 12 is inserted into the uterus, which is a smooth muscle infused with water.

In the next step 102, the state of the RAM 38 is monitored and whether the internal pressure data input from the pressure transducer 12 via the amplifier 14 and A / D converted by the pressure transducer I / F 34 is input. If the internal pressure data is not input, the process waits. If the internal pressure data is input, in step 104, the internal pressure data is plotted as a graph of time and voltage, and the CRT 28
Show this graph above.

FIG. 4 shows, as an example, a graph showing the internal pressure data sampled at 0.1 kHz as an average value per second.

At the next step 106, the state of the RAM 38 is monitored to judge whether or not the treatment data including the medication treatment name and the treatment time is inputted from the keyboard 26, and if the treatment data is not inputted, the step is carried out. Proceed to step 112, and if the treatment data is input, step 1
A number (serial number) is added to the treatment data input in 08,
The treatment time is read from the treatment data, and the read treatment time and the assigned number are marked on the graph (see FIG. 4). In the next step 110, this treatment data is made into a table as shown in Table 1 together with the assigned numbers, and this is displayed on the CRT 28, and step 11
Proceed to 2.

[0015]

[Table 1]

In step 112, the state of the RAM 38 is monitored to determine whether or not the input of the internal pressure data from the pressure transducer I / F 34 is completed. If the input is not completed, the process returns to step 104. When the input is completed, the state of the RAM 38 is monitored in step 114 to determine whether or not the data addition command is input from the keyboard 26. When the data addition command is not input, the process proceeds to step 120, and when the data addition command is input, the data addition condition is acquired in step 116. The data adding conditions, arbitrary time T ₁ as a reference of the addition, the time T ₁ before the addition time interval T ₂ of the order to perform the number of additions Z after time T _1, for performing addition after time T ₁ like reference voltage value such as a time interval T ₃ and 0 levels of. Next step 118
The internal pressure data is added on the basis of the data addition condition input in step S12, the addition result is displayed on the CRT 28, and step 12
Go to 0.

In Table 2, T ₁ = each treatment time in Table 1, T ₂
= 15 minutes, T ₃ = 15 minutes, Z = 2 times, and the horizontal line A in FIG.
Is the reference voltage value, the total sum of the internal pressure data that exceeds the horizontal line A is shown.

[0018]

[Table 2]

In step 120, the state of the RAM 38 is monitored to determine whether an FFT (Fast Fourier Transform) processing command has been input from the keyboard 26. FF
Step 1 if no T processing command is input
In step 28, if the FFT processing command is input, the FFT processing condition is acquired in step 122. FF
Examples of the T processing condition include an arbitrary time T ₄ and an arbitrary time width T _{5 in} which FFT should be performed. Next Step 1
FFT processing is performed on the internal pressure data based on the FFT processing conditions input at 24, specifically, the power value (dB) of the spectrum for each frequency component is obtained from this internal pressure data, and this spectrum is displayed on the CRT 28, Go to step 126.

FIG. 5 is a power spectrum for each frequency component when a value obtained by subtracting 30 minutes from the treatment time of Treatment 1 in Table 1 is used as T ₄ , and 30 minutes is used as the time width T ₅ . Further, FIG. 6 is a power spectrum for each frequency component when the treatment time of the treatment 1 of Table 1 is used as T _{4 and} 30 minutes is used as the time width T ₅ . As the time width T ₅ , for example, 10 seconds to 30 seconds, 0.5 minutes to
Any biological reaction cycle (time) such as 2 minutes and 2 minutes to 10 minutes may be used. Further, for example, a value obtained by subtracting 30 minutes from the treatment time of the treatment 1 of Table 1 as T ₄ is the time width T
FFT processing is performed using ₅ as 0 to 30 minutes, 10 seconds to 30 seconds, 0.5 minutes to 2 minutes, and 2 minutes to 10 minutes, and CRT
28 may display the spectrum of T ₅ = 0 to 30 minutes, that is, only FIG.

In the next step 126, the total sum of the power values is obtained, the obtained total sum of the power values is displayed on the CRT 28, and the process proceeds to step 128. At this time, step 124
It is also possible to display only on the CRT 28 those that have been FFT-processed and the spectrum has not been displayed on the CRT 28.

In Table 3, 10 seconds to 30 when the value obtained by subtracting 30 minutes from the treatment time of Treatment 1 of Table 1 is used as T _4.
The sum of each power value between seconds, 0.5 minutes to 2 minutes and 2 minutes to 10 minutes is shown, and Table 4 shows 10 seconds when the treatment time of the treatment 1 of Table 1 is used. ~ 30 seconds, 0.5 minutes ~
The sum of each power value between 2 minutes and 2 minutes to 10 minutes is shown. Comparing FIG. 5 and FIG. 6, a qualitative difference between the two is recognized, and the quantitative difference can be understood by comparing Tables 3 and 4.

[0023]

[Table 3]

[0024]

[Table 4]

In step 128, the internal pressure data, A / D
The transducer sampling period and treatment data are stored on the disc set in the disc drive 32 and the routine ends.

As described above, according to the present invention, the internal pressure data converted from the contraction / relaxation reaction of smooth muscle can be automatically converted into the power spectrum for each frequency component,
The operability of measuring smooth muscle contraction and relaxation reaction can be improved. Further, according to the present invention, recording paper is unnecessary, and continuous measurement can be performed for a long time.

Further, according to the present invention, since the sum of the power values for each biological reaction cycle is obtained, a quantitative difference which is difficult to judge only by the power spectrum is judged by comparing the sum of the power values. can do. Further, in the present invention, since the treatment time is linked and displayed on the graph showing the internal pressure data of FIG. 4, the measurer can easily determine the biological reaction to the applied stimulus. Specifically, the present invention automatically converts a time-series digital signal converted from a biological response to a stimulus into a power spectrum and an integrated value of the time-series digital signals before and after the reaction, whereby the existing It is possible to detect a change in biological reaction that cannot be determined by the method, and improve the operability of biological reaction measurement.
For example, in the biological reaction shown in FIG. 4, a slight change (a rise in contraction frequency without a rise in peak after treatment 1) is visually recognized before and after treatment 1, but in the present invention, it is shown in Table 2. Integrated value of time series digital signal as shown,
As a result of being quantitatively displayed as Table 3 (integrated value of each power spectrum for 30 minutes before treatment 1) and Table 4 (integrated value of each power spectrum for 30 minutes after treatment 1), Table 2 15 minutes after treatment 1 and about 15 to 30 minutes 15 minutes before treatment 1, about 3 minutes each compared to 15 minutes before treatment 1.
It was found that there was a 2-fold, approximately 2-fold increase in contraction, and Table 3
From the integrated value of each power spectrum in Table 4 as a contraction period, there is almost no change in the contraction period of 10 to 30 seconds before and after the treatment for 30 minutes, and 0.5 to 2 minutes, 2
It can be read that the contraction components for 10 minutes increase by about 1.7 times and about 2.2 times, respectively. Thus, the present invention
By providing a method of more accurately quantifying a biological reaction to a stimulus, the excellent effect that the effect of the stimulus can be determined more accurately is exerted.

Further, the above-mentioned power spectrum measuring apparatus 1
The effect of the present invention in comparison with the existing method will be described using another data obtained by 0.

When examining the uterine contractile action of a uterine contractile drug such as prostaglandin F2α, the existing method could not detect the dose dependence considering the frequency and the strength of contraction, and the uterine contraction was first expressed. Although the potency of the drug was compared by the drug concentration and the like, according to the present invention, the dose dependence can be detected more quantitatively by using the integrated amount of the reaction and the sum of the power spectra for each cycle zone. As a result, more accurate information can be obtained about the efficacy between drugs.

Specifically, in the reactions shown in FIG. 7, in the treatments 2, 3, 4, 5, 6, and 7, the drug concentrations were 0.01, 0.03, 0.1, 0. 3, 1, 3μ
Although it is different from mol / l, in the evaluation of only the maximum value by the existing method, 0.01 μmol in FIG. 8 (treatments 2 to 7 from the left).
/ L, the reaction amount increased to 1.6, after which the change in the dose-response curve was gradual, indicating that the dose-responsiveness was poor. By using the sum of the power spectra (0.5-2 minutes), a very good dose response could be obtained.

In addition to the above, in the present invention, the internal pressure data, A /
The sampling period of the D converter and the treatment data are stored in the disk, and the stored data can be read and the read data can be used for general-purpose spreadsheet software or the like.

In the above embodiment, the data addition and FFT processing are performed before the data is stored in the disk. However, after the data is stored in the disk, the necessary data is read from this disk and the data addition and the FFT processing are performed. You can go.

In the above embodiment, data averaging may be performed instead of data addition or together with data addition to obtain an average value of internal pressure data at a designated time.

Further, in the above embodiment, the result of the FFT processing is displayed as the ratio (dB), but the processing result may be displayed as the absolute value, the normalized value or the constituent percentage of the power spectrum.

Further, although the internal pressure measuring method for uterine contraction is shown in the above-mentioned embodiment, the target living organ may be the digestive tract, blood vessels or other smooth muscles, and the method for detecting biological reaction. Is not limited to pressure measurement, and may be contraction force measurement using a strain gauge or isometric displacement gauge, or organ length or thickness measurement using an isotonic displacement gauge or ultrasonic wave.

The strain gauge is suitable for measuring the contraction of the muscle itself by sewing directly to an organ or the like in the living body, and is particularly effective for examining an object having an anisotropic contraction force such as smooth muscle. is there. Since the pressure transducer indirectly measures the contraction of muscle through water as in the above embodiment, the anisotropy cannot be examined, but the tissue is small like the rat uterus and strain gauges cannot be used. It is effective in some cases.

The isometric displacement meter and the isotonic displacement meter are effective means for taking out an organ and testing the organ alone.

The stimuli given to the living body include mechanical, thermal and physical stimuli as well as chemical stimuli such as medication.

[0039]

INDUSTRIAL APPLICABILITY According to the present invention, a time series digital signal converted from a biological response to a stimulus is automatically converted into a periodic spectrum, which improves the operability of the biological response measurement and, at the same time, improves dose response and the like. It has an excellent effect that it is possible to perform a quantitative evaluation and that the measurer can easily judge the effect of the stimulus based on the periodic spectrum.

The advantage of being able to detect the dose response is that it is possible to quantitatively determine the relative potency of the test drug with respect to the standard drug. According to the present invention, standard uterine contractions such as prostaglandin F2α It is possible to accurately determine the dose of the test drug having the same potency as the drug, and to provide an extremely effective means for setting the dose and estimating the effect in clinical trials.

[Brief description of the drawings]

FIG. 1 is a schematic diagram of a power spectrum measuring apparatus according to an embodiment of the present invention.

FIG. 2 is a flowchart showing a control routine of a CPU.

FIG. 3 is a continuation of the flowchart in FIG. 2;

FIG. 4 is a first graph displayed on a CRT created by the power spectrum measuring apparatus according to the embodiment of the present invention.

FIG. 5 is a power spectrum for each frequency component from 30 minutes before the procedure 1 to the procedure 1 which is calculated by the FFT by the power spectrum measuring apparatus according to the embodiment of the present invention and displayed on the CRT.

FIG. 6 is a power spectrum for each frequency component from procedure 1 to 30 minutes after procedure 1 calculated by FFT and displayed on the CRT by the power spectrum measuring apparatus according to the embodiment of the present invention.

FIG. 7 is a second graph created by the power spectrum measuring apparatus according to the embodiment of the present invention and displayed on a CRT.

FIG. 8 is a graph showing the sum of the peak value measured by the existing method, the integrated reaction value and the power spectrum measured by the present invention in treatments 2 to 7 of FIG.

[Explanation of symbols]

 10 Power spectrum measuring device 12 Pressure transducer 22 CPU

Claims (2)

[Claims] 1. A spectrum measuring device comprising: a digital converting means for converting a biological response to a stimulus into a time-series digital signal; and a spectrum converting means for converting the converted digital signal into a periodic spectrum. 2. A digital conversion step of converting a biological response to a stimulus into a time-series digital signal, a spectrum conversion step of converting the converted digital signal into a periodic spectrum, and based on a periodic spectrum of a specific range. A spectrum measuring method comprising: a determining step of determining the effect of stimulation.