JPH0892585A - Production of physiologically active yolk oil - Google Patents

Abstract

PURPOSE: To obtain at a low cost a large quantity of yolk oil retaining physiological activity such as antihyperlipemic action, antidiabetic action, antiallergic action or antisenile action, by subjecting iodine-rich egg's yolk to extraction with ethyl alcohol. CONSTITUTION: This yolk oil is obtained by subjecting the yolk of iodine-rich eggs (e.g. 600-1000μg iodine/egg) obtained by feeding poultry with a feed formulated with sea algae or an iodine compound (e.g. calcium iodide) to extraction with ethyl alcohol. For example, this yolk oil is obtained by the following process: chickens are fed with a feed formulated with 100ppm, on an iodine basis, of calcium iodate, and the eggs delivered at least one week after feeding are gathered, and the yolk therefrom is dried by a combination of microwave and hot-air drying, and 6wt. times of ethyl alcohol is added to the resultant dry yolk followed by agitation for one hour and then centrifugal filtration; the filtrate is filtered through a filter cloth followed by distilling the ethanol off using a distillation unit.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method for producing egg yolk oil which is extracted from an egg yolk containing a high iodine content without impairing physiological activity.

[0002]

2. Description of the Related Art Egg yolk oil has long been known as a nourishing tonic and good for heart disease. Such egg yolk oil is a blackish brown tar-like liquid obtained by collecting egg yolk from eggs and heating the egg yolk in an iron pan or the like. There is a research report that egg yolk oil obtained by such heat treatment has mutagenicity (carcinogenicity), which may cause a problem in long-term intake.

On the other hand, a method of chemically extracting egg yolk oil using an organic solvent is also known, which is obtained by adding n-hexane, dimethyl ether and the like to egg yolk and extracting and purifying it, which is distributed as egg yolk lecithin. are doing. This egg yolk lecithin is used in the fields of medicine, cosmetics, foods, etc. by extracting lecithin which is highly contained in egg yolk and utilizing its emulsifying property. Such egg yolk lecithin has no special physiological activity other than its emulsifying property, and since it is expensive to manufacture, soybean lecithin, which is inexpensive, has been replaced as a raw material.

[0004]

Conventionally, no biological activity other than emulsifying property has been known in the yolk of poultry eggs such as hen's eggs and parrot eggs. On the other hand, iodine-rich eggs obtained by feeding a diet containing a high amount of iodine such as seaweed are known to have physiological activities such as anti-hyperlipidemic action, anti-diabetic action, anti-allergic action and anti-aging action. (Japanese Patent Publication No. 63-13967, Japanese Patent Publication No. 63-53163, Japanese Patent Publication No. 63-3237, Japanese Patent Publication No. 1-48248). However, a method for specifically extracting these physiologically active components from the iodine-rich egg is not known. The present invention provides a method for extracting egg yolk oil containing a large amount of a physiologically active ingredient at a low cost from the egg yolk of a poultry egg containing a large amount of iodine.

[0005]

[Means for Solving the Problems] The inventors of the present invention have conducted extensive studies in order to achieve the above-mentioned object, and no physiological activity was observed in extraction of commonly used egg yolk lipids with n-hexane, which is an extraction solvent. The present invention has been completed by confirming that dimethyl ether cannot be used for foods, but that egg yolk lipids extracted with ethyl alcohol have the above-mentioned various physiological activities.

[0006] That is, the present invention has a physiological activity characterized by extracting with ethyl alcohol from the yolk of an iodine-rich egg obtained by feeding poultry with a feed containing seaweed and / or an iodine compound. It is a method of producing egg yolk oil.

The egg yolk used in the present invention contains seaweed or an iodine compound recognized as a feed additive in poultry feed in an amount of 20 ppm to 2500 pp as an iodine content in the feed.
m, preferably 30 to 100 ppm of iodine is mixed so that it is collected from an egg which is laid and laid on a chicken, a quail or the like.

When such an iodine-rich feed is fed to poultry, particularly laying hens during egg collection, the target eggs containing a large amount of iodine are produced after about 1 week. For example, in the case of laying hens, if a feed containing iodine of about 100 ppm is administered, it will be about 600 to 1000 μ per egg.
Eggs containing about g of iodine are produced.

The thus-obtained iodine-rich egg is broken into eggs, and the yolk obtained by separating the yolk and egg white is dried to obtain a dried yolk. As the drying method, any drying method such as freeze drying, microwave drying, hot air drying, and heating roll drying may be used. The water content is preferably 20% or less. Next, twice or more ethyl alcohol is added to the dried egg yolk to extract the lipid in the egg yolk. The concentration of ethyl alcohol is preferably 70% or more, and 85% is preferable in view of economy and extraction efficiency.

It is desirable to carry out the extraction operation usually in a tank equipped with a stirrer and repeat it a couple of times. The target egg yolk is obtained by removing ethyl alcohol from the extract obtained by filtration under reduced pressure. Oil is obtained. Extracting egg yolk oil with ethyl alcohol in this way makes it possible to produce egg yolk oil having physiologically active effects such as anti-hyperlipidemia, anti-diabetes, anti-inflammatory and anti-allergy.

[0011]

[Example] Calcium iodate as iodine in feed 1
It was added at 00 ppm and fed to 3000 laying chickens. 3000 eggs after 1 week were collected to obtain 55 kg of raw egg yolk containing a high iodine content. The iodine content in this egg yolk was 20 ppm. This egg yolk was dried by a combination of microwave and hot air drying to obtain 25.6 kg of dried egg yolk. About 6 times the amount of ethyl alcohol (water content 0.43%) is added to this, and after stirring for about 1 hour, centrifugal filtration is performed to separate into a filtrate and a residue. The same amount of ethyl alcohol was further added to the residue, the mixture was stirred for 1 hour, and the filtrate obtained by the same filtration was combined with the previously collected filtrate. This was further filtered using a filter cloth, and ethyl alcohol was removed using a distillation apparatus to obtain about 10.4 kg of egg yolk oil.

In order to examine the anti-inflammatory effect of the egg yolk oil containing a large amount of iodine obtained as described above, an arachidonic acid-induced inflammation test using mice was carried out. That is, 3 groups of 10 ddY mice were provided, and 200 μg of iodine-rich yolk oil was added to the right auricle of mice under light anesthesia with ether.
Was dissolved in 30 μl of ethanol and applied. In addition, as a comparative example, 200 μg of yolk oil from common eggs extracted in the same manner as in the above example was applied in the same manner. Further, 200 μg of NDGA (anti-inflammatory drug) was applied as a positive control.

One hour after the application of these yolk oil and NDGA, light anesthesia was carried out again with ether, and 30 μ of arachidonic acid / acetone solution (100 mg / 1.5 ml) was applied to the right auricle.
1 was applied. Immediately thereafter, 0.1 ml of 1% Evans blue dye solution was intravenously administered to 10 g of body weight through the tail vein. After applying arachidonic acid for 90 minutes to induce inflammation,
The amount of Evans blue dye leaked into the pinna was quantified.

As a result, the yolk oil area of general eggs was 22.3 ±.
6.1 μg, the yolk oil with high iodine content of the present invention is 14.7
± 9.3 μg, NDGA group was 14.6 ± 4.3 μg, and the yolk oil of the iodine-rich egg of the present invention showed the same anti-inflammatory effect as the positive control drug.

[0015]

Since the egg yolk oil of the present invention extracts the egg yolk oil using ethyl alcohol, the physiology such as the anti-inflammatory action, anti-allergic action, anti-diabetic action, anti-hyperlipidemic action, etc. possessed by the iodine-rich egg is There is an effect that a large amount of egg yolk oil can be produced inexpensively without impairing the activity.

─────────────────────────────────────────────────── ─── Continuation of the front page (51) Int.Cl. ⁶ Identification code Office reference number FI technical display location A61K 33/18 ABE ABF ADN ADP AGZ 35/54 7431-4C

Claims (1)

[Claims] 1. Production of a yolk oil having a physiological activity, characterized in that the egg yolk of an iodine-rich egg obtained by feeding a feed containing seaweed and / or an iodine compound to poultry is extracted with ethyl alcohol. Method.