JPH0881381A - Health and beauty-promoting agent having sod action, blood pressure-suppressing agent and constipation-improving agent - Google Patents

Abstract

PURPOSE: To obtain a health and beauty-promoting agent having safety and excellent SOD actions (an action effectively removing O2 <-> thought as harmful for an organism and an action preventing disease, etc.) by using rice brans and soybeans as raw materials. CONSTITUTION: This health and beauty-promoting agent having the SOD action is characterized by blending 100 pts.wt. of distillation residue of a concentrated solution (e.g. a concentration of 40-50wt.%) of a fermented solution prepared by seeding Bacillus subtilis in a medium containing rice brans and soybeans, cultivating and filtering with 1-50 pts.wt. of extracted essence of green tea (e.g. a concentration of 40-50wt.%) as a value reduced to distillation residue. The fermented solution may contain flavonoid, and phytic acid or its salt may contain a green tea-extracted essence and the distillation residue (solid residue) may be used instead of the above solutions. This agent is also usable as a blood pressure-suppressing agent and a constipation-improving agent.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a health / beauty enhancer having SOD action, a blood pressure suppressor and a constipation improver.
The present invention relates to various diseases caused by active oxygen (O ₂ ⁻ ), for example, diseases caused by blood flow disorder (myocardial infarction, stroke,
High blood pressure, menstrual cramps, stiff shoulders, neuralgia, backache, hangover, etc., adult diseases / medical diseases (cancer, nephritis, hepatitis, diabetes, etc.), beauty / skin diseases (stains, freckles, rough skin, coldness, constipation, wrinkles, atopic nature) It is widely used for the treatment and improvement of dermatitis, etc.).

[0002]

2. Description of the Related Art Superoxide dismutase (SOD
Say. ) Is a superoxide radical (superoxide radical) generated by one-electron reduction of an oxygen molecule.
cal, O ₂ ^- disproportionation) (lower formula) catalyzed at a rate close to diffusion control, O ₂ in the cell ^- an enzyme to reduce the concentration. O ₂ ⁻ + O ₂ ⁻ + 2H ⁺ → H ₂ O ₂ + O _{2 If} O ₂ ⁻ is harmful to the living body in recent years, this SOD, which is a scavenging enzyme, should exist to protect the living body from active oxygen toxicity. Therefore, the reaction mechanism, physiological mechanism, and the like are being studied from the viewpoint of being useful for diseases and the like that are considered to be caused by this active oxygen [[reactive oxygen-production / elimination in living organisms Molecular mechanism of action "(2 new editions, published by Kyoritsu Shuppan Co., Ltd., edited by Minoru Nakano) P. Two
23-230)]. In addition, there is a fact that SOD activity is low in cancer cells, and further, although a direct causal relationship between SOD and carcinogenesis is not clear, it is reported that injection of SOD or SOD-like substances into cancer cells suppresses proliferation (description). (P.64).

[0003]

[Problems to be Solved by the Invention] Safe and SOD action (The SOD action here means not only the action of decreasing the active oxygen concentration but also the prevention and improvement of various diseases which are considered to be caused by this. If there is a food having the effect of (1), it will be extremely useful for human health and beauty, and there is a great need for it. In particular, in a situation where stress is high and many diseases are occurring as in recent years, the necessity thereof is great.

The present invention uses rice bran and soybeans as raw materials, and is a health and beauty enhancer having a safe and excellent SOD action, an antihypertensive agent and a constipation improving agent, and at least phytic acid and its salts. An object of the present invention is to provide a health / beauty enhancer or the like having an SOD action, which comprises one side and a green tea extract.

[0005]

From the above viewpoints, the inventors of the present invention diligently investigated whether or not there is a safe fermented substance used as a health / beauty enhancer having SOD activity. Upon investigation, it was discovered that by adding a predetermined additive to the fermentation liquor obtained by fermenting using the above-mentioned raw materials and filtering, it was possible to achieve the object, which led to the completion of the present invention. . The health / beauty enhancer having an SOD effect of the first invention is a fermentation liquor produced by inoculating Bacillus subtilis into a medium containing rice bran, soybeans and water, culturing and filtering, or an evaporation residue of the fermentation liquor. In addition, the green tea extract extract or the evaporation residue of the green tea extract extract is contained in each minute. A sixth invention uses the health / beauty enhancer of the first invention as a blood pressure suppressor, and the eleventh invention uses the health / beauty enhancer as a constipation improving agent.

The "green tea extract" is obtained by extracting tea leaves using methanol, ethanol, ethyl acetate and the like (particularly methanol and ethanol) as a solvent, and the concentration of the extracted components is about 5 to 50% by weight. be able to. And, as shown in the second invention, the mixing ratio of the green tea extract can be 1 to 50 parts by weight in terms of the evaporation residue, based on 100 parts by weight of the evaporation residue of the fermentation liquid. . This is because if it is less than 1 part by weight, the effect cannot be expected sufficiently, and if it exceeds 50 parts by weight, it becomes difficult to taste and drink. In addition, in the third, eighth, and thirteenth inventions, the green tea extract is extracted with methanol, ethanol, or a mixed solution of methanol / ethanol to be water-soluble.

The health / beauty enhancer having an SOD action of the fourth aspect of the present invention is a fermentation liquor produced by inoculating Bacillus subtilis in a medium containing rice bran, soybeans and water, culturing and filtering, or the fermentation broth. It is characterized in that flavonoids are contained in the evaporation residue of. A ninth aspect of the invention uses the health / beauty enhancer of the fourth invention as a blood pressure suppressor, and a fourteenth aspect of the invention uses the health / beauty enhancer as a constipation-improving agent. The "flavonoid" is widely distributed in the plant kingdom, the two phenyl groups are bonded across the three carbon atoms of the pyran ring, or close to it the structure, so-called C ₆ -C ₃ -C ₆ It is a group of compounds consisting of carbon skeleton. This flavonoid is contained in 13 to 14% by weight based on the evaporation residue in the green tea extract. The blending ratio of the flavonoid can be about 0.1 to 7 parts by weight with respect to 100 parts by weight of the fermentation broth (for example, when the extract concentration is 40 to 50% by weight). This is because if the amount is less than 0.1 part by weight, the effect cannot be expected sufficiently, and if it exceeds 7 parts by weight, it becomes difficult to taste and drink.

The above-mentioned "rice bran" means rice germ, defatted rice germ, rice bran, defatted rice bran and the like, and "soybeans" means defatted soybean, quina flour, soybean flour, soybean dregs and hydrolysates thereof. Etc. Usually, these are added in an amount of 10 to 20 parts by weight of soybeans when the rice bran is 100 parts by weight. The fermentation conditions are usually pH of 7.5 to 10 (especially around 9) and culture temperature of about 40 to 45 ° C. In addition, protease can be used as a medium material. This case is useful because it further decomposes the soybean peptide.

The "fermentation solution" may be a solution obtained by culturing the cultured culture fermentation solution as it is, a solution that has been subjected to post-treatment such as decolorization, or a concentrated solution obtained by concentrating the solution. As described above, the evaporation residue (solid content) of this fermentation liquid may be used. The health / beauty enhancer having an SOD action of the fifth aspect of the present invention is characterized by containing at least one of phytic acid and a salt thereof and a green tea extract (or an evaporation residue of the green tea extract). To do. Also,
A tenth invention uses the health / beauty enhancer of the fifth invention as a blood pressure inhibitor, and a fifteenth invention uses the health / beauty enhancer as a constipation improving agent.

The above-mentioned "phytic acid" may be synthetic phytic acid or phytic acid extracted and isolated. Also,
As the “salt”, a non-toxic salt is used, and a salt with a metal, an organic base, a basic amino acid, an organic ester residue, etc. is used, and examples thereof include potassium, sodium, arginine,
It may be a salt of ornithine, lysine, histidine, glucamine, monoethanolamine or the like. Since this phytic acid exhibits acidity, a salt thereof having a pH of about 6 to 8 is usually used. It does not matter whether this salt is water-soluble or not,
Since a health / beauty enhancer or the like is often used as an aqueous solution, a water-soluble one is preferable. Further, this phytic acid and its salts are contained in a large amount in the fermentation broth, and when converted from the phosphorus concentration, 6 to 7 parts by weight of the phytic acid equivalent is contained with respect to 100 parts by weight of the evaporation residue. There is. Therefore, as in the fifth, tenth, and fifteenth inventions, even if a predetermined amount of green tea extract is contained in phytic acid or the like, as in the case of the first invention, the same effect is surely obtained. It is considered to be a thing.

[0011]

The present invention will be described in detail with reference to the following examples. (1) Production of Fermentation Liquid and Preparation of Test Product of Health Food (Health / Beauty Promoter etc.) First, a fermentation liquid is produced by the following method. That is,
Defatted rice bran; 30 kg, soybean meal (or defatted soybean); 3 k
g, bittern, 5 kg, water; 500 kg were used. In addition, p
H is around 9. The above medium was sterilized at 121 ° C. for 30 minutes, then cooled, and then inoculated with 0.05 Kg of natto bacterium (manufacturer; Naruse Fermentation and Chemical Research Institute), aeration and stirring at 40 to 45 ° C. for about 48 hours. And cultured.

Thereafter, this culture was squeezed and filtered, treated with activated carbon and perlite to deodorize and decolorize, and an almost transparent fermentation liquor (fermented extract) was obtained. As the activated carbon, various types such as powdered activated carbon (activated carbon S, activated carbon K, etc.) and granular activated carbon (activated carbon SG, etc.) can be used, and as the perlite, “Perlite No. 4180” (Daikaline Orient Co., Ltd.) Manufactured) was used. This fermented liquid is a colorless or pale yellow transparent liquid, has a flavor of fermentation, water content of 92 to 94% (according to normal pressure heating and drying method), and phosphorus of about 300 to 400 mg / 100 g (according to vanad molybdenum analysis method). In general, live bacteria, Escherichia coli, mold, yeast, arsenic, lead, cadmium and tin were not detected.

As the test product 1 of this health food, 3 parts by weight of the green tea extract (methanol or ethanol extract, solid content concentration; 47: 100 parts by weight of the fermentation liquid) was used.
˜48% by weight, specific gravity; 1.07 to 1.09) (evaporation residue ratio: fermentation liquor content / extracted extract content = 100:
20) was used. The solid content concentration was determined as a Brix value, and the concentration of ethanol and glycerin (mainly ethanol) was about 50% by weight, and the concentration of water was about 2.6% by weight. Moreover, it is considered that this green tea extract contains a considerable amount of flavonoids.

(2) Performance Evaluation (A) SOD Action Under the following conditions, a test on H ₂ O ₂ (Table 1,
Table 2), a test for OH (Table 3), and a test in the O ₂ -xanthine system (Table 4) were performed, and the results are shown in each table. As a test product, a 5-fold diluted solution with water was used as the test product 1.

[0015]

[Table 1]

Table 1 --------------------------------------------- [Example 1] H ₂ O ₂ --- −−−−−−−−−−−−−−−−−−−−−−−−−−−−−−− 0 min 10 min Average / min PLCO mol / min −−−−−−−−− −−−−−−−−−−−−−−−−−−−−−−−−−− control product 5 50 2.5 348 −−−−−−−−−−−−−−−−− −−−−−−−−−−−−−−−−− Test product 1 75 73 0.2 28 −−−−−−−−−−−−−−−−−−−−−−−− −−−−−−−−−−−

[0017]

[Table 2]

Table ₂ --- ------------------------------------- [Example 2] H ₂ O ₂ --- −−−−−−−−−−−−−−−−−−−−−−−−−−−−−−− 0 min 10 min Average / min PLCO mol / min −−−−−−−−− −−−−−−−−−−−−−−−−−−−−−−−−−− control product 75 23 5.2 723 −−−−−−−−−−−−−−−− −−−−−−−−−−−−−−−−−− Test product 1 75 70 0.5 70 −−−−−−−−−−−−−−−−−−−−−−−− −−−−−−−−−−−

[0019]

[Table 3]

Table 3 −−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−− OH sample −−−−−−−−−−−− ---------------- [Example 1] [Example 2] --------------------------------------- --- STIMULATER / INCUBATION 40 minutes 40 minutes ----------------------------------- −−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−− Test product 1 153.0 1.87 −−−−−−−−− −−−−−−−−−−−−−−−−−−−−−−−−−

[0021]

[Table 4]

Table 4 −−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−− O ₂ -xanthine system ---------- −−−−−−−−−−−−−−−−−−−−−−−−−− Sample O. D. (NM) nM / min −−−−−−−−−−−−−−−−−−−−−−−− C (2 min-1 min) C-Black −−−−−−−−−− −−−−−−−−−−−−−−−−−−−−−−−− control product 0.0251 1.190 −−−−−−−−−−−−−−−−−−− --------------------- Test product 1 0.0244 0.0205 0.972 ------------------------------ −−−−−−−−−−

As shown in Tables 1 to 4 above, this test product 1
Indicates that the SOD action is superior to that of the control product. From the above, the SOD activity was 46
It can be said that it is 0 unit / ml (1 cup 10 ml). Furthermore, the following tests were conducted for the synergistic effect of the fermented liquid and the green tea extract. That is, each sample (No. A to C) having the following blending ratio was prepared. --------------------------------------- No. A Fermentation liquid 100 g ----------------------------- No. B green tea extract 1 g Purified water 99 g -------------------- No. C Fermented liquid 99 g Green tea extract 1 g ------------------------------- SOD activity was measured by the following measurement method for each of the above samples. Measured [Method of measuring SOD activity] This method is a method using an EPR (electron spin resonance) device, and is recently performed as a test item; "superoxide scavenging activity" at the Japan Food Research Center. That's how it became. That is, first, to 1 g of each homogenized sample, 0.1 M phosphate buffer solution (p
H7.8) is added and extraction is sufficiently performed to obtain an extract. Then, the following reagents are mixed. 2 mM hypoxanthine / phosphate buffer 50 μl 5.5 mM DETAPAC (diethlenetriaminepentaacetic a
cid) / phosphate buffer 35 μl extract or standard SOD (superoxide dismutase) 50 μl DMPO (5,5-dimethyl-1-pyrroline-1-oxide) 15 μl 0.4 units / ml xanthine oxidase / phosphate buffer 50 μl , This mixed solution was sucked into a special flat cell (made by JEOL Ltd., capacity: about 130 μl) and set in an ESR device (JES-FR80 type made by JEOL Ltd.), 40
The sweep was started after 2 seconds, and the measurement was performed under the ESR operation conditions shown below. In addition, this SOD activity measurement is a measurement of the solution one day after the sample preparation. [ESR operation conditions] temperature room temperature microwave output 8 mW magnetic field 334.7 mT ± 5 mT modulation 100 kHz, 0.79 × 0.
1 mT Amplification factor 3.2 × 100 Response time 0.1 seconds Sweep time 2 minutes Then, the ratio between the peak height of Mn ^{2+ and} the peak height of the measurement signal is obtained. Next, standard SOD (0 to 15 units / ml)
A calibration curve was prepared in step 1 and the values obtained with the extract were interpolated to calculate the erasing activity in the sample. The results are as follows. The unit is UNIT / g. “Activity additive theoretical value” is the sample No. A
And SOD activity values of B are simply integrated. -------------------------------------------- A 3.965 × 10 ³ −−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−− No. B 0.660 × 10 ³ −−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−− No. C 5.300 * 10 < ³ > (Activity addition theoretical value 4.590 * 10 < ³ >) -----------------------. ----- No. containing the fermented liquid and the green tea extract. In C, the actual SOD activity value is 1.15 times the active additive theoretical value, which indicates that there is a synergistic effect.

(B) Fluctuations in blood pressure during elevated blood pressure in rats Under the following conditions, 100 parts by weight of the fermentation broth was mixed with 1.5 parts by weight of the green tea extract to give a test product 2. The change in blood pressure due to the administration of this test product 2 was evaluated. The result is shown in FIG.

[Test conditions] Animal: rat; 3 males for control, 4 males for test product,
7 in total. The age of this rat is 6 weeks, and its blood pressure is 150 mmHg or higher. It should be noted that this rat has a genetic property that blood pressure increases from 3 weeks of age (hereinafter referred to as SH
Called R. ). Test administration method: Free intake by water bottle. Indoor: Temperature 23 ± 1 ° C, humidity 50% ± 10%. Beverage: Glucose and citric acid were added to a 10% solution of the test product 2 (corresponding to SOD) to make it easy to drink. Blood pressure measurement; once every 2 weeks. Feed used: Common feed (“MF” manufactured by Oriental Yeast Co., Ltd.) was used in all cases. The results show that the use of this test product 2 has an effect of suppressing an increase in blood pressure as compared with the control group.

(C) Fluctuation of blood pressure in the hypertensive completion stage of rats The fluctuation of blood pressure due to administration of Test Product 2 was evaluated under the same conditions as above except the following conditions. The result is shown in FIG. [Test Conditions] Animal: rat (above SHR); control: 4 males, test product: 4 males, 8 in total. And the age of this rat is 12
At 15 weeks of age, its blood pressure is 180 mmHg or higher.
The other conditions are the same as above. According to this result, as shown in FIG. 2, it has an effect of suppressing hypertension and, at the same time, an excellent suppressing effect with the passage of administration time.

(D) Monitor test (1) Regarding the constipation-improving effect, a monitor test was conducted as follows, and the following good results were obtained. This test method is intended for 50 women with constipation (the number of valid answers is 49), and 5 ml of the stock solution (fermented solution) is ingested within 30 minutes of waking up. As a result, 38 women had bowel movements on the first day and all 49 women had bowel movements on the second and third days.

(2) Further, 60 parts by weight of the fermentation broth, 1.2 parts by weight of the green tea extract and 14 parts by weight of oligosaccharide,
The following good results were obtained when a monitor test was conducted in which a test product (portion containing 10 ml) mainly composed of purified water was used for humans. A (58 years old, female) [Symptoms before the start of the monitor] His face was dark, the climbing of stairs was very difficult, his blood pressure was 165, and he was constantly taking a depressant. [Results after 2 drinks daily] My legs became lighter in about 1 week. The antihypertensive drug was stopped and the blood pressure became 130 in about 3 weeks. After two months, my face turns white so much that I can't see it. The 10-year-old started to look younger.

B (70 years old, male) [Symptoms before the start of the monitor] Even in everyday life, my cardiopulmonary function was weak, so I felt like I was out of breath when I climbed the stairs and dragged my legs. [Results after 3 daily drinks (morning, noon, evening)] In about 2 weeks, the body begins to feel light. After about a month, I can't breathe out even when I climb the stairs. Four months later, when I was asked to see cardiopulmonary function at the hospital, it was much better. For the first time this winter, I was fine without having to be hospitalized without catching a cold.

C (52 years old, female) [Symptoms before starting monitoring] He was suffering from diabetes and constipation due to high blood pressure. The blood pressure is 180 at the top and 95 at the bottom. [Results after 2 daily drinks (for 2 months) then 1 daily drink]
From the 4th day, urine is about twice as much as before and my body is feeling better. The stools will also appear once every three days. Then every two days. Two months later, my doctor tells me that I don't have to take blood pressure lowering drugs. His blood pressure was 145 on the upper side and 78 on the lower side, and he no longer needed medicine.

D (23 years old, female) [Symptoms before the start of monitoring] Constipation was severe 1 since childhood.
She had a flight once every two weeks from the 0th day. There were always small pimples on the face, and the skin was dry.
It was rather slender, but the lower abdomen was protruding like an infant figure, and I was considerably worried. [Results after 5 drinks for the first 2 days and 2 drinks every day] The following morning, there was a bowel movement. There are daily flights than that. After a month and a half, my face was completely smooth and my skin was shiny. The makeup glue has improved very much. E (70 years old, female) [Symptoms before starting monitoring] He was hospitalized for high blood pressure of 200. [Results after 2 daily drinks (morning and evening)] Blood pressure dropped in about 10 days and I was able to be discharged from the hospital. Currently 140.

F (68 years old, female) [Symptoms before starting monitoring] I was tired and tired, and my body was also stiff. [Results after 2 daily drinks (morning and evening)] Moisturized skin and improved makeup paste from the 2nd day. I was less tired than in the second week and I started to move as I wanted. G (45 years old, male) [Symptoms before starting monitoring] Diabetes was frequently hospitalized for 2 to 3 weeks, and the liver was hurt during the treatment. [Results after 2 daily drinks] After 2 months, my doctor told me that my liver had completely improved, and after 4 months I had better diabetes, and I was able to get back to work by returning to work. There is no need for hospitalization.

(E) Summary of Effects of Examples As described above, the present fermentation liquor is safe because harmful bacteria and heavy metals are not recognized, and it is a natural fermented product of completely natural raw materials. As shown in the above results, the SOD action is excellent, and thus the blood pressure suppressing effect is excellent as described above. In addition, since it is excellent in this SOD action,
In addition to the blood pressure suppressing effect, it can be said that the effects resulting from this SOD action are expected in the same manner. Furthermore, the present inventors believe that this fermented liquid has an action of effectively incorporating the green tea extract into the body. In this regard, it is necessary to wait for further detailed research, but in any case, if this fermentation broth and green tea extract are used in combination, the SOD action is excellent and it can be safely consumed by humans, and it is difficult for the human body. Clearly useful. Further, since both the fermented liquor and the green tea extract are water-soluble, they can be dissolved in water as shown in the above-mentioned test to be used as an aqueous solution drink, food, etc. Therefore, it can be said that they are well absorbed in the body. Furthermore, as shown in the monitor test of (D), it is tested as a beverage (food) in which this test product (aqueous solution) is put in a potion (a small container shape containing fresh milk) type container. It is also easy to make various aqueous products (beverages, drinks, etc.).

The present invention is not limited to the specific examples described above, and various modifications may be made within the scope of the present invention depending on the purpose and application. That is,
The form of the health enhancer or the like consisting of the fermented liquor or the like and the green tea extract or the like is usually a liquid such as an aqueous solution or an undiluted solution as shown in the above Examples, but not limited to this, the liquid health enhancer Powder products impregnated with liquid-absorbent powder, granulated granulated products, tablets blended with other powder components (filler, etc.), or microcapsules, soft capsules, hard capsules, candy, jelly, and biscuits. Etc. can be filled or mixed. In addition, a product form in which a predetermined container is filled with these aqueous solution or powder product, or used alone or in combination with other agent (whether aqueous solution, oily liquid or powder) Whether or not it is not particularly limited, for example, it may be a potion type as mentioned in the above-mentioned examples, may be filled in a container having another shape, or a powder product may be filled in a stick-shaped container (bag). These may be used, or may be further mixed with and dispersed in conventional soft drinks, drinks, dairy products, oil-based products and the like. The dispersion may be water-in-oil type or oil-in-water type.

[0035]

EFFECTS OF THE INVENTION Since the health and beauty promoting agent is excellent in SOD action, it is also excellent in the effects resulting from the SOD action (for example, blood pressure suppressing effect). Therefore, by using this health and beauty enhancer, various diseases caused by active oxygen, for example,
Diseases caused by blood flow disorders (myocardial infarction, stroke, high blood pressure, menstrual cramps, stiff shoulders, neuralgia, back pain, hangover, etc.), adult diseases / internal diseases (cancer, nephritis, hepatitis, diabetes, etc.), beauty / skin diseases (stains, freckles) , Rough skin, chills, constipation, wrinkles, atopic dermatitis, etc.), etc., and is considered to exhibit excellent effects. Further, the blood pressure inhibitor and the constipation improving agent of the present invention are excellent in the effect of suppressing blood pressure and the effect of improving bowel movements, respectively. Further, in the case of a water-soluble health / beauty enhancer or the like, it has an excellent absorption effect, and its application field is broadened as a water-soluble beverage or food.

Further, in particular, the fermented liquor used as the main component in the present invention is a natural fermented product of a completely natural raw material, so that harmful bacteria and heavy metals are not recognized, and it is safe and originally promotes health and beauty. It is a very well-balanced health and beauty enhancer since it is made from rice bran and soybean products which are useful as a raw material and contains useful nitrogen compounds (amino acids etc.), ash and phosphorus compounds. Furthermore, since the green tea extract is also a natural product, the safety of health and beauty enhancer containing it is excellent.

[Brief description of drawings]

FIG. 1 is a graph showing changes in blood pressure during SHR blood pressure elevation in an example.

FIG. 2 is a graph showing fluctuations in blood pressure in the SHR hypertension completion period in Examples.

─────────────────────────────────────────────────── ─── Continuation of the front page (51) Int.Cl. ⁶ Identification code Internal reference number FI technical display location // A23F 3/16

Claims (15)

[Claims] 1. A fermented liquor produced by inoculating Bacillus subtilis into a medium containing rice bran, soybeans, and water, culturing and filtering, or the evaporation residue of the fermented liquor, to which the green tea extract or the green tea extract is added. SO containing the evaporation residue of
Health and beauty enhancer with D action. 2. The green tea extract is 1 in terms of the evaporation residue, based on 100 parts by weight of the evaporation residue of the fermentation liquor.
The health / beauty enhancer having an SOD action according to claim 1, which is mixed in an amount of 50 parts by weight. 3. The health / beauty enhancer according to claim 1, wherein the green tea extract is a water-soluble extract extracted from at least one of methanol and ethanol. 4. A fermentation liquor produced by inoculating a culture medium containing rice bran, soybeans and water with Bacillus subtilis, culturing and filtering the mixture, or an evaporation residue of the fermentation liquor containing flavonoids. A health and beauty enhancer with a characteristic SOD action. 5. A health / beauty enhancer having an SOD action, comprising at least one of phytic acid and a salt thereof, and a green tea extract or an evaporation residue of the green tea extract. 6. A green tea extract or said green tea extract in a fermentation liquor produced by inoculating a culture medium containing rice bran, soybeans and water with Bacillus subtilis, culturing and filtering, or an evaporation residue of the fermentation liquor. An antihypertensive agent comprising the evaporation residue of 7. The green tea extract extract is 1 in 100 parts by weight of the evaporation residue of the fermentation broth, calculated as the evaporation residue.
The blood pressure suppressor having the SOD effect according to claim 6, which is mixed in an amount of -50 parts by weight. 8. The blood pressure suppressant according to claim 6, wherein the green tea extract is a water-soluble extract extracted from at least one of methanol and ethanol. 9. A flavonoid is contained in a fermentation liquor produced by inoculating Bacillus subtilis into a medium containing rice bran, soybeans and water, culturing and filtering, or the evaporation residue of the fermentation liquor. Characteristic blood pressure suppressant. 10. An antihypertensive agent comprising at least one of phytic acid and a salt thereof, and a green tea extract or an evaporation residue of the green tea extract. 11. A fermented liquor produced by inoculating Bacillus subtilis into a medium containing rice bran, soybeans and water, culturing and filtering the mixture, or an evaporative residue of the fermented liquor, a green tea extract or the green tea extract. A constipation-improving agent characterized by containing the evaporation residue of. 12. The constipation-improving agent according to claim 11, wherein the green tea extract extract is added in an amount of 1 to 50 parts by weight based on 100 parts by weight of the evaporation residue of the fermentation broth in terms of the evaporation residue. 13. The constipation improving agent according to claim 11, wherein the green tea extract is a water-soluble extract extracted from at least one of methanol and ethanol. 14. A fermentation liquor produced by inoculating Bacillus subtilis into a medium containing rice bran, soybeans and water, culturing and filtering the mixture, or an evaporation residue of the fermentation liquor containing flavonoids. Characteristic constipation improver. 15. A constipation-improving agent comprising at least one of phytic acid and a salt thereof and a green tea extract or an evaporation residue of the green tea extract.