JPH0870812A - New full-bodied seasoning raw material - Google Patents

New full-bodied seasoning raw material

Info

Publication number
JPH0870812A
JPH0870812A JP6212280A JP21228094A JPH0870812A JP H0870812 A JPH0870812 A JP H0870812A JP 6212280 A JP6212280 A JP 6212280A JP 21228094 A JP21228094 A JP 21228094A JP H0870812 A JPH0870812 A JP H0870812A
Authority
JP
Japan
Prior art keywords
extract
seasoning
raw material
tropomyosin
troponin
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP6212280A
Other languages
Japanese (ja)
Other versions
JP3317041B2 (en
Inventor
Keigo Shima
圭吾 島
Norihiko Yamada
典彦 山田
Motohisa Kuroda
素央 黒田
Tsutomu Harada
努 原田
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ajinomoto Co Inc
Original Assignee
Ajinomoto Co Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ajinomoto Co Inc filed Critical Ajinomoto Co Inc
Priority to JP21228094A priority Critical patent/JP3317041B2/en
Priority to EP95103973A priority patent/EP0672354B1/en
Priority to DE69517836T priority patent/DE69517836T2/en
Priority to US08/407,368 priority patent/US5679397A/en
Publication of JPH0870812A publication Critical patent/JPH0870812A/en
Application granted granted Critical
Publication of JP3317041B2 publication Critical patent/JP3317041B2/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Abstract

PURPOSE: To obtain a seasoning raw material containing troponin and tropomyosin as raw materials and capable of producing a substance providing firm, thickness and body characteristic of natural raw material by heating the raw material in various kinds of extract seasonings. CONSTITUTION: This seasoning raw material contains troponin and tropomyosin which occupy about 5% of protein in animal muscle. When the raw material is heated in various extract seasonings, a high polymer component which is a substance providing firm, thickness and body is produced by reaction of the three components comprising a low molecular weight fraction of extract content of natural extract and/or its substituent. The raw material is useful for obtaining a full-bodied seasoning capable of providing firm, thickness and body which natural raw materials such as beef soup stock or bonito broth have to beverage or food.

Description

【発明の詳細な説明】Detailed Description of the Invention

【0001】[0001]

【産業上の利用分野】本発明は、トロポニン、トロポミ
オシンおよび天然エキスのエキス分の低分子画分を水中
で加熱することにより「しまり」および「あつみ、こ
く」付与物質が生成する現象を利用した「しまり」およ
び「あつみ、こく」付与機能を有する調味料素材および
コク味調味料に関する。
The present invention takes advantage of the phenomenon in which low-molecular-weight fractions of troponin, tropomyosin, and natural extracts are heated in water to produce "Shimari" and "Atsumi, Koku" imparting substances. The present invention relates to a seasoning material having a function of imparting "shimari" and "atsumi, koku" and a rich seasoning.

【0002】[0002]

【従来の技術】各種料理のベースとして、畜肉エキス、
チキンエキス、魚介類エキス、野菜エキスなどの天然エ
キスが業務用として広く用いられている。これらの天然
エキスの機能は、食品の味全体をひきしめ、複雑な味と
幅を与える、すなわち、「しまり」および「あつみ、こ
く」を与える、食品材料の味の不足を補うなどとされて
いる。
2. Description of the Related Art As a base for various dishes, meat extract,
Natural extracts such as chicken extract, seafood extract and vegetable extract are widely used for commercial use. The functions of these natural extracts are said to tighten the overall taste of foods and give them a complex taste and breadth, that is, to give "tightness" and "atsumi, koku", and to supplement the lack of taste of food ingredients. .

【0003】しかし、これらの天然エキスは高価であ
り、入手しがたいものであるため、一般的には、これら
天然エキスの一部を用いた加工品および代替品が製造・
市販され利用されている。また、かつお節、煮干、だし
昆布、シイタケなどの基本だし、およびこれらの天然材
料に食塩、砂糖、うま味調味料、アミノ酸などを配合し
た風味調味料も広く使われている。これら市販の天然エ
キスの加工品および代替品などは、その組成がグルタミ
ン酸ナトリウムを中心としたアミノ酸、核酸、有機酸な
どの低分子物質を主成分に構成されているために、やは
り天然のエキスと比較してみると、呈味が単純であり、
ぼけているという欠点を有している。
However, since these natural extracts are expensive and difficult to obtain, generally, processed products and substitute products using a part of these natural extracts are produced and manufactured.
It is commercially available. In addition, basic stocks such as bonito flakes, niboshi, dashi kelp and shiitake mushrooms, and flavor seasonings in which these natural ingredients are mixed with salt, sugar, umami seasoning, amino acid and the like are also widely used. Since these commercially available processed products and substitutes of natural extracts are composed mainly of low molecular weight substances such as amino acids such as sodium glutamate, nucleic acids, and organic acids, they are also natural extracts. By comparison, the taste is simple,
It has the drawback of being blurred.

【0004】従来、このような欠点を補うためには、H
VP(植物蛋白加水分解物)、HAP(動物蛋白加水分
解物)、酵母エキス等を添加することにより、コク味、
複雑味を付与し、呈味の改善を計っているが、HVPお
よびHAPは、分解臭を有しているために、また、酵母
エキスは、酵母特有の風味を有しているため、自ずから
その使用量に制限が生じ、いわゆるモデルとした天然エ
キスとは明らかに呈味・風味が異なり天然感、複雑感に
欠けると言う欠点を有し満足できるものではなかった。
特に、味全体をととのえて味をひきしめる、「しま
り」、ならびに、後味の伸びや深みを与える「あつみ、
こく」といった言葉で示されるような呈味において顕著
な違いが有るという問題点を有している。
Conventionally, in order to make up for such drawbacks, H
By adding VP (plant protein hydrolyzate), HAP (animal protein hydrolyzate), yeast extract and the like, a rich taste,
Although a complex taste is imparted and the taste is improved, HVP and HAP have a decomposed odor, and yeast extract has a flavor peculiar to yeast. The amount used was limited, and the taste and flavor were clearly different from the so-called natural extract used as a model, which was unsatisfactory because it lacked a natural and complex feeling.
In particular, "Shimari", which brings out the overall taste and tightens the taste, and "Atsumi, which gives the aftertaste the breadth and depth"
There is a problem that there is a marked difference in taste as indicated by the word "Koku".

【0005】[0005]

【発明が解決しようとする課題】前項記載の従来技術の
背景下に、本発明は、飲食品にビーフブイヨンや鰹節だ
し汁などの天然素材の持つ「しまり」および「あつみ、
こく」を付与するための新規な調味料素材、コク味調味
料、並びに「しまり」および「あつみ、こく」付与方法
を提供することを目的とする。
DISCLOSURE OF THE INVENTION Under the background of the prior art described in the preceding paragraph, the present invention provides "Shimari" and "Atsumi", which are natural ingredients such as beef broth and bonito soup stock for food and drink.
It is an object of the present invention to provide a novel seasoning material for imparting "koku", a rich seasoning, and a method for imparting "shimari" and "atsumi, koku".

【0006】[0006]

【課題を解決するための手段】本発明者は、上記課題の
解決につき鋭意工夫を重ねた結果、トロポニンおよびト
ロポミオシンを各種エキス調味料中で加熱を行うと、天
然素材特有の「しまり」および「あつみ、こく」を有す
る、新規「しまり」および「あつみ、こく」付与機能を
有する高分子成分が生成すること、しかもこの物質はト
ロポニン、トロポミオシンおよび天然エキスのエキス分
の低分子画分の3者の反応により生ずるものであること
を見いだし、このような知見に基づいて本発明を完成す
るに至った。
[Means for Solving the Problems] As a result of earnest efforts to solve the above problems, the present inventor has found that when troponin and tropomyosin are heated in various extract seasonings, "tightness" and "specific" The production of a new high molecular component having "atsumi, koku" and a function of imparting new "shimari" and "atsumi, koku", and this substance is a member of troponin, tropomyosin and low molecular weight fractions of natural extracts. It was found that the reaction is caused by the reaction of, and the present invention has been completed based on such knowledge.

【0007】なお、本発明に言う「しまり」および「あ
つみ、こく」とは、ビーフブイヨンやかつお節だし汁な
どの天然素材の持つ呈味質であり、味全体をひきしめる
とともに後味の伸びおよび深みを表現するものである。
このような呈味質は上記に示した、グルタミン酸ナトリ
ウムなどのアミノ酸類、イノシン酸ナトリウムやグアニ
ル酸ナトリウムなどの核酸関連化合物およびHVP、H
APや酵母エキスなどの調味料素材では再現できないも
のである。
The "Shimari" and "Atsumi, Koku" referred to in the present invention are the taste qualities of natural materials such as beef broth and bonito soup stock, and they express the extension and depth of the aftertaste as well as the overall taste. To do.
Such taste qualities are shown above, such as amino acids such as sodium glutamate, nucleic acid-related compounds such as sodium inosinate and sodium guanylate, and HVP and H.
It cannot be reproduced with seasoning materials such as AP and yeast extract.

【0008】以下、本発明を詳細に説明する。The present invention will be described in detail below.

【0009】本発明の実施態様の第1は、トロポニンお
よびトロポミオシンを含有することを特徴とする新規な
コク味調味料素材に関する。このようなコク味調味料
は、このままの形態で流通に置くことができる。
A first embodiment of the present invention relates to a novel rich seasoning ingredient containing troponin and tropomyosin. Such a rich seasoning can be put into the distribution as it is.

【0010】トロポニンおよびトロポミオシンは、周知
のように、種々の動物や菌類から抽出して得ることので
きる物質であるが、本発明にはその由来のいずれのもの
も使用可能である。トロポニンおよびトロポミオシン
は、筋収縮・弛緩のカルシウムイオンによる制御を媒介
する調節蛋白としてよく知られており、両者とも動物の
筋肉中の蛋白の約5%を占めることも、これまた周知の
通りである。
As is well known, troponin and tropomyosin are substances that can be obtained by extraction from various animals and fungi, and any of those derived from them can be used in the present invention. It is also well known that troponin and tropomyosin are well known as regulatory proteins that mediate the regulation of muscle contraction / relaxation by calcium ions, and both occupy about 5% of proteins in animal muscle. .

【0011】本発明の実施態様の第2は、トロポニンお
よびトロポミオシンに加えて、天然エキスのエキス分の
低分子画分及び/又はその代替物をも含有することを特
徴とする新規なコク味調味料素材に関する。このような
コク味調味料素材もまた、この形態で流通に置くことが
できる。
[0012] A second embodiment of the present invention is a novel seasoning for kokumi, characterized in that, in addition to troponin and tropomyosin, it also contains a low molecular weight fraction of the extract of natural extract and / or its substitute. Regarding the material. Such a rich seasoning ingredient can also be put into distribution in this form.

【0012】トロポニンおよびトロポミオシンは、上に
説明した通りである。
Troponin and tropomyosin are as described above.

【0013】天然エキスのエキス分の低分子画分は、要
するに、トロポニンおよびトロポミオシンと水中で加熱
したときに「しまり」および「あつみ、こく」付与機能
を有する高分子物質を与えることのできる低分子画分
で、例えばビーフエキスの場合はゲル濾過カラム「トヨ
パールHW−55F」(東ソー(株)製)により測定し
たときの分画分子量約10,000以下の画分である。
このような低分子画分は、天然エキスから分取したもの
でもよいが、未分取のビーフエキス、ポークエキスやチ
キンエキスなどの畜肉系エキス調味料、カツオエキス、
サバエキス、ホタテガイエキスやアサリエキスなどの魚
介系エキス調味料および酵母エキス等の天然エキスその
ままの形態でも使用可能であり、さらにまた、天然エキ
スを配合した配合エキスの形態、或いは、アミノ酸混合
物等の天然エキス代替物や天然エキスと天然エキス代替
物との配合品等でも使用可能である。
The low molecular weight fraction of the extract of the natural extract is, in short, a low molecular weight compound capable of giving a high molecular substance having a function of imparting "Shimari" and "Atsumi, Koku" when heated in water with troponin and tropomyosin. In the case of beef extract, for example, it is a fraction having a molecular weight cutoff of about 10,000 or less as measured by a gel filtration column "Toyopearl HW-55F" (manufactured by Tosoh Corporation).
Such a low molecular weight fraction may be obtained by fractionating from a natural extract, but unfractionated beef extract, meat extract seasoning such as pork extract and chicken extract, skipjack extract,
It is also possible to use the natural extract such as mackerel extract, scallop extract, clam extract, and other seafood-based seasonings and yeast extract as it is, and also the form of a compounded extract containing a natural extract, or a natural extract such as an amino acid mixture. It is also possible to use an extract substitute or a mixture of a natural extract and a natural extract substitute.

【0014】本発明の実施態様の第3は、(1)トロポニ
ン、(2)トロポミオシンおよび(3)天然エキスのエキス分
の低分子画分及び/又はその代替物を水中で加熱するこ
とにより製造されたことを特徴とする新規なコク味調味
料に関する。
A third embodiment of the present invention is prepared by heating a low molecular weight fraction of the extract of (1) troponin, (2) tropomyosin and (3) natural extract and / or its substitute in water. The present invention relates to a novel rich flavor seasoning.

【0015】このようなコク味調味料、すなわちビーフ
ブイヨンやかつお節だし汁などの天然素材に特有の「し
まり」および「あつみ、こく」を付与する機能を有する
コク味調味料は、例えば、以下に示す方法で得ることが
可能である。トロポニンおよびトロポミオシンをカツオ
エキスやビーフエキスなどの調味料溶液中に溶解し、
「しまり」および「あつみ、こく」付与機能を有する高
分子物質の生成するに適当な条件で加熱する。このよう
な条件は、例えば、50〜150℃における、1〜5時
間の加熱である。なお、このときのトロポニンの添加濃
度は溶液に対して例えば0.01〜10%(上乗せ、固
形物換算)、そしてトロポミオシンの添加濃度は0.0
1〜10%(上乗せ、同上)である。また、加熱時にお
いて、エキス調味料溶液の固形分濃度を、例えば、5〜
80%に調整して反応を行なうが、特にこの濃度に限定
されるものではない。
Examples of such rich seasonings, that is, rich seasonings having a function of imparting "tightness" and "atsumi, koku" peculiar to natural materials such as beef broth and bonito soup stock are shown below. Can be obtained by the method. Dissolve troponin and tropomyosin in a seasoning solution such as skipjack extract and beef extract,
Heating is performed under conditions suitable for producing a polymer substance having a function of imparting "tightness" and "atsumi, koku". Such conditions are, for example, heating at 50 to 150 ° C. for 1 to 5 hours. The addition concentration of troponin at this time is, for example, 0.01 to 10% with respect to the solution (added, converted to solid matter), and the addition concentration of tropomyosin is 0.0.
It is 1 to 10% (added, same as above). Further, when heated, the solid content concentration of the extract seasoning solution is, for example, 5 to
The reaction is performed by adjusting the concentration to 80%, but the concentration is not particularly limited.

【0016】加熱後の反応液は、そのままで、または適
宜、透析、限外濾過あるいはエタノール沈澱などの方法
を用いて、高分子画分すなわち蛋白質を中心とした画分
を回収して、本発明の調味料素材が製造される。前者の
場合、すなわち、加熱反応後の調味料溶液はそのままの
状態で「しまり」および「あつみ、こく」の増強された
調味料溶液となる。
The reaction solution after heating may be used as it is, or may be appropriately subjected to a method such as dialysis, ultrafiltration or ethanol precipitation to recover a high molecular weight fraction, that is, a protein-centered fraction. The seasoning material is manufactured. In the former case, that is, the seasoning solution after the heating reaction becomes a seasoning solution in which “Shimari” and “Atsumi, Koku” are enhanced in the state as it is.

【0017】このようにして得られた「しまり」および
「あつみ、こく」を付与する物質は、日本料理のだし、
たとえば、かつお節、鶏肉、魚貝、こんぶ、牛肉、シイ
タケなどの素汁に添加し、西洋料理のスープストック、
たとえば、牛肉、鶏肉、豚肉、魚貝などの素汁に添加
し、中華料理のタン、たとえば、牛肉、鶏肉、豚肉、ハ
ム、貝柱、アワビ、エビ、スルメ、シイタケ、ハクサ
イ、セロリなどの素汁に添加することにより、これらに
「しまり」および「あつみ、こく」を付与し、その呈味
機能を増強させることが判明した。また、前述のごと
く、上記の天然エキスの加工品および代替品、特にアミ
ノ酸混合物として比較的安価に利用できるHVP、HA
P、酵母エキスに添加したり、低品質の安価なビーフエ
キスに添加した場合にも、また基本だし素材または従来
の風味調味料に添加しまたはこれと併用した場合にも、
味全体をまとめ、「しまり」および「あつみ、こく」を
付与しまたはこれを増強するとともに、これらを高品質
なものに改良することができる。
The substances that give "Shimari" and "Atsumi, Koku" obtained in this way are Japanese food stocks,
For example, add it to the broth of bonito flakes, chicken, fish and shellfish, kelp, beef, shiitake mushrooms, soup stock of Western dishes,
For example, it is added to the soup of beef, chicken, pork, fish and shellfish, and the tan of Chinese foods such as beef, chicken, pork, ham, scallop, abalone, shrimp, squid, shiitake mushrooms, Chinese cabbage, celery, etc. It was clarified that when added to the above, "Shimari" and "Atsumi, Koku" were added to these to enhance the taste function. Further, as described above, processed products and substitutes of the above-mentioned natural extracts, especially HVP and HA which can be used at a relatively low cost as a mixture of amino acids.
P, when added to yeast extract or added to low-quality, inexpensive beef extract, or when added to basic stock ingredients or conventional flavor seasonings, or in combination therewith,
The whole taste can be put together and added or enhanced with "tightness" and "atsumi, koku", and these can be improved to high quality.

【0018】尚、「しまり」および「あつみ、こく」を
付与する物質の濃度は、これを添加使用する対象とする
飲食品、調味料などに応じてその至適使用量の範囲が異
なるが、当業者であれば簡単な事前トライアルにより適
当な使用量を極めて容易に定めることができる。本発明
者の経験では、例えば、液中濃度が0.005%〜2%
となるように添加することにより、従来の調味料素材な
どに欠けていた「しまり」および「あつみ、こく」を付
与し、味全体をととのえ、味をひきしめることにより味
のぼけを抑制することができた。
The concentration of the substance that gives "Shimari" and "Atsumi, Koku" varies depending on the food and drink, the seasoning, etc. to which it is added. Those skilled in the art can very easily determine an appropriate amount to be used by a simple preliminary trial. According to the experience of the present inventor, for example, the concentration in liquid is 0.005% to 2%.
By adding it so that it will add the "tightness" and "atsumi, koku" that were lacking in conventional seasoning materials, etc., the taste can be suppressed and the blurring of the taste can be suppressed by tightening the taste. did it.

【0019】本発明の実施態様の第5は、飲食品または
その原材料を、(1)トロポニン、(2)トロポミオシンおよ
び(3)天然エキスのエキス分の低分子画分及び/又はそ
の代替物の中の少なくとも1種を添加しかつこれら(1)
〜(3)の3種全ての存在下に加熱することにより「しま
り」および「あつみ、こく」が付与されまたは増強され
て製造されたことを特徴とする「しまり」および「あつ
み、こく」を有する飲食品に関する。
In a fifth embodiment of the present invention, a food or drink or a raw material thereof is used as a low molecular fraction of (1) troponin, (2) tropomyosin and (3) natural extract and / or a substitute thereof. At least one of these and (1)
~ (3) "Shimari" and "Atsumi, Koku" are produced by adding or enhancing "Shimari" and "Atsumi, Koku" by heating in the presence of all three types. Regarding food and drinks that they have.

【0020】このような「しまり」および「あつみ、こ
く」を有する飲食品には、いわゆる調味料素材そのもの
も含まれることは、本発明の、これまでに説明した性質
上、明らかである。
It is clear from the properties of the present invention described above that foods and drinks having such "Shimari" and "Atsumi, Koku" include so-called seasoning materials themselves.

【0021】また、このような「しまり」および「あつ
み、こく」を有する飲食品の製造法についても、飲食品
またはその原材料を、(1)トロポニン、(2)トロポミオシ
ンおよび(3)天然エキスのエキス分の低分子画分及び/
又はその代替物の少なくとも1種を添加しかつ3種全て
の存在下に、これら3種から「しまり」および「あつ
み、こく」付与機能を有する物質が生成する条件下で加
熱することを除いては、特別の制限はなく、適宜従来の
飲食品の製造法に準ずることができる。
[0021] Further, regarding the method for producing a food or drink having such "Shimari" and "Atsumi, Koku", the food or drink or the raw material thereof is prepared from (1) troponin, (2) tropomyosin and (3) natural extract. Low molecular weight fraction of extract and /
Or at least one of its alternatives, and in the presence of all three, except under the conditions that a substance having a function of imparting "tightness" and "atsushi, koku" is produced from these three Is not particularly limited and can be appropriately applied to the conventional method for producing food and drink.

【0022】このような製造法によれば、既存の飲食品
に「しまり」および「あつみ、こく」を付与することが
でき、または既に付与されていた「しまり」および「あ
つみ、こく」を増強することもできる。また、飲食品の
原材料から飲食品を加熱して製造する場合には、差し仕
えがなければ、この加熱工程に上記の「しまり」および
「あつみ、こく」付与機能を有する物質を生成せしめる
加熱をかねさせることができる。
According to such a production method, "Shimari" and "Atsumi, Koku" can be added to the existing food or drink, or "Shimari" and "Atsumi, Koku" already added can be enhanced. You can also do it. Further, in the case of producing foods and drinks by heating them from the raw materials of foods and drinks, if there is no waiting, heating to generate the substance having the above-mentioned "tightness" and "atsumi, koku" imparting function in this heating step. Can be imitated.

【0023】本発明に係わる「しまり」および「あつ
み、こく」付与機能を有する物質は、先に説明したよう
に、(1)トロポニン、(2)トロポミオシンおよび(3)天然
エキスのエキス分の低分子画分及び/又はその代替物の
3種を水中で加熱することにより生成する。従って、本
発明に係わる「しまり」および「あつみ、こく」を有す
る飲食品の製造法において、既存の飲食品または飲食品
の原材料には、少なくとも「しまり」および「あつみ、
こく」付与機能を有する物質の生成しうる程度の水分の
含まれていることまたは添加することを前提とする。
As described above, the substance having a function of imparting “tightness” and “atsumi, koku” according to the present invention has a low content of (1) troponin, (2) tropomyosin and (3) natural extract. It is produced by heating the molecular fraction and / or its three alternatives in water. Therefore, in the method for producing a food or drink having "Shimari" and "Atsumi, Koku" according to the present invention, at least "Shimari" and "Atsumi" are included in the existing food or drink or the raw material of the food or drink.
It is premised that the substance having the function of imparting "bodily" contains or is added with water to the extent that it can be produced.

【0024】また、前記3種は、これらを全てあらため
て添加する必要はなく、既存の飲食品または飲食品の原
材料に既に存在している場合は、それをそのまま利用す
ることができる。ただし、これら3種のうち、少なくと
も1者はあらためて添加しなければならないことは言う
までもない。
Further, it is not necessary to add all of the above three kinds, and if they are already present in existing food or drink or raw materials for food or drink, they can be used as they are. However, it goes without saying that at least one of these three must be added again.

【0025】[0025]

【実施例】以下に、天然素材に特有の「しまり」および
「あつみ、こく」を付与する物質を得る方法とその添加
効果を実施例をあげて説明する。 なお、本発明の技術
的範囲はこれら実施例によって制限されるものではない
ことはもちろんである。
[Examples] A method for obtaining a substance that imparts "tightness" and "atsumi, koku" peculiar to natural materials and the effect of addition thereof will be described below with reference to examples. Of course, the technical scope of the present invention is not limited to these examples.

【0026】[0026]

【実施例1】牛肉から西田らの方法(ジャーナル オブ
バイオロジカル ケミストリイ(Journal of Biologi
cal Chemistry), 261, 16749 (1986))により調製した
トロポニン250mgおよびトロポミオシン250mg
を市販ビーフエキス調味料溶液500mlに溶解し、鍋
で95℃において、3時間加熱を行った。このときのエ
キス調味料溶液の固形分濃度を予め15%に調整してお
いた。加熱後の反応液について水に対して透析を行い、
透析膜内液(高分子画分:分子量10,000以上)す
なわち蛋白質を中心とした画分約0.4g(乾重量)を
得た。
[Example 1] From the method of beef to Nishida et al. (Journal of Biologi
cal chemistry), 261, 16749 (1986)) troponin 250 mg and tropomyosin 250 mg
Was dissolved in 500 ml of a commercially available beef extract seasoning solution and heated in a pan at 95 ° C. for 3 hours. The solid content concentration of the extract seasoning solution at this time was adjusted to 15% in advance. The reaction solution after heating is dialyzed against water,
A solution in the dialysis membrane (polymer fraction: molecular weight of 10,000 or more), that is, a fraction mainly containing protein (0.4 g, dry weight) was obtained.

【0027】このようにして得られた物質を市販コンソ
メスープに0.01%、0.05%、0.1%、0.2
%となるように上乗せ添加し、その呈味をパネル5名に
てプロファイル評価した。この結果、上記物質を添加し
たサンプルは、いずれもコントロールである市販コンソ
メスープのもつ風味を変える事なく、「あつみ、こく」
の増強に加えて、いわゆる味全体をひきしめる「しま
り」を付与する事が判明した。しかし、その添加効果
は、0.05%以上の添加では大差がみられず、経済的
なことを考えると0.1%程度の添加で充分であると推
定された。
The thus obtained substance was added to a commercial consomme soup in 0.01%, 0.05%, 0.1% and 0.2%.
%, And the taste was profile-evaluated by 5 panelists. As a result, all the samples to which the above substances were added did not change the flavor of the commercial consomme soup, which is a control, and could be treated as "atsumi, koku".
It was found that in addition to the enhancement of "," the "tightness" that gives the so-called overall taste is added. However, with respect to the effect of addition, a large difference was not observed with the addition of 0.05% or more, and it was estimated that the addition of about 0.1% was sufficient from the economical point of view.

【0028】[0028]

【実施例2】実施例1と同様の方法により調製したトロ
ポニン250mgおよびトロポミオシン250mgを市
販酵母エキス調味料溶液250mlに溶解し、鍋で95
℃において、3時間加熱を行った。このときのエキス調
味料溶液の固形分濃度を予め30%に調整して反応を行
った。加熱後の反応液について水に対して透析を行い、
透析膜内液に生成した濁り物質(イオン強度が低下する
事により塩溶性蛋白が凝集した物)を遠心分離機(約1
0,000xg、20分)により集め、約0.1g(乾
重量)を得た。
[Example 2] 250 mg of troponin and 250 mg of tropomyosin prepared by the same method as in Example 1 were dissolved in 250 ml of a commercially available yeast extract seasoning solution, and the solution was heated in a pan at 95%.
Heating was carried out at 0 ° C for 3 hours. At this time, the solid content concentration of the extract seasoning solution was adjusted to 30% in advance to carry out the reaction. The reaction solution after heating is dialyzed against water,
A turbid substance (a salt-soluble protein aggregated due to a decrease in ionic strength) generated in the dialysis membrane liquid was centrifuged (about 1
50,000 × g, 20 minutes) to give about 0.1 g (dry weight).

【0029】このようにして得られた物質を市販コンソ
メスープに0.1%となるように上乗せ添加し、その呈
味をパネル5名にてプロファイル評価した。この結果、
上記物質を添加したサンプルは、いずれもコントロール
である市販コンソメスープのもつ風味を変える事なく、
「あつみ、こく」の増強に加えて、いわゆる味全体をひ
きしめる「しまり」を付与する事が判明した。
The substance thus obtained was added to a commercially available consomme soup in an amount of 0.1%, and the taste was profile-evaluated by 5 panelists. As a result,
Samples added with the above substances, without changing the flavor of commercial consomme soup, which is a control,
In addition to the enhancement of "Atsumi, Koku", it has been found that "Shimari" which gives a so-called overall taste is added.

【0030】[0030]

【実施例3】市販ビーフカレースープ0.5Lに対し
て、実施例1と同様の方法で得たトロポニン約0.3g
を添加し、95℃で4時間加熱を行った。比較のため、
トロポニンを添加しなかった以外は全く同様に処理して
対照スープを得た。
[Example 3] About 0.3 g of troponin obtained in the same manner as in Example 1 with respect to 0.5 L of commercially available beef curry soup
Was added and heated at 95 ° C. for 4 hours. For comparison,
A control soup was obtained by the same treatment except that troponin was not added.

【0031】両者を実施例と同様の方法にて官能検査に
供した結果は、下記表1に示す通りである。
The results of sensory tests of both of them in the same manner as in the example are shown in Table 1 below.

【0032】[0032]

【表1】 [Table 1]

【0033】[0033]

【発明の効果】以上のように示した方法により、トロポ
ニンおよびトロポミオシンを各種エキス調味料中におい
て加熱することによって、「しまり」および「あつみ、
こく」といった言葉で示されるコク味を付与する調味料
素材を得ることが可能であった。
EFFECTS OF THE INVENTION By the above-described method, troponin and tropomyosin are heated in various extract seasonings to give "Shimari" and "Atsumi,"
It was possible to obtain a seasoning material that imparts a rich taste represented by the word "koku".

フロントページの続き (72)発明者 原田 努 神奈川県川崎市川崎区鈴木町1−1 味の 素株式会社食品総合研究所内Front page continuation (72) Inventor Tsutomu Harada 1-1 Suzuki-cho, Kawasaki-ku, Kawasaki-shi, Kanagawa Ajinomoto Co., Inc. Food Research Institute

Claims (5)

【特許請求の範囲】[Claims] 【請求項1】 トロポニンおよびトロポミオシンを原料
とする調味料素材。
1. A seasoning material made from troponin and tropomyosin as raw materials.
【請求項2】 トロポニンおよびトロポミオシンに加え
て、天然エキスのエキス分の低分子画分及び/又はその
代替物をも含有することを特徴とする請求項1記載の新
規なコク味調味料素材。
2. The novel kokumi seasoning material according to claim 1, characterized in that, in addition to troponin and tropomyosin, it also contains a low molecular weight fraction of natural extract and / or its substitute.
【請求項3】 請求項2の素材を水中において加熱する
ことにより製造されたことを特徴とする新規なコク味調
味料。
3. A novel rich flavor seasoning produced by heating the material of claim 2 in water.
【請求項4】 請求項3記載のコク味調味料を配合され
ていることを特徴とする天然エキス加工品、天然エキス
代替物、基本だし素材又は風味調味料。
4. A natural extract processed product, a natural extract substitute, a basic broth ingredient, or a flavor seasoning, which is blended with the rich flavor seasoning according to claim 3.
【請求項5】 飲食品またはその原材料を、(1)トロポ
ニン、(2)トロポミオシン、(3)天然エキスのエキス分の
低分子画分及び/又はその代替物の少なくとも1種を添
加しかつ3種全ての存在下に加熱することにより「しま
り」および「あつみ、こく」が付与されまたは増強され
て製造されたことを特徴とする「しまり」および「あつ
み、こく」を有する飲食品。
5. A food or drink or a raw material thereof is added with at least one of (1) troponin, (2) tropomyosin, (3) a low molecular weight fraction of an extract of natural extract and / or its substitute, and 3 Foods and drinks having "Shimari" and "Atsumi, Koku" characterized by being produced by adding or enhancing "Shimari" and "Atsumi, Koku" by heating in the presence of all the seeds.
JP21228094A 1994-03-18 1994-09-06 New kokumi seasoning material Expired - Lifetime JP3317041B2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP21228094A JP3317041B2 (en) 1994-09-06 1994-09-06 New kokumi seasoning material
EP95103973A EP0672354B1 (en) 1994-03-18 1995-03-17 Proteinaceous material for enhancing food taste quality
DE69517836T DE69517836T2 (en) 1994-03-18 1995-03-17 Protein-containing agent to improve the taste quality of food
US08/407,368 US5679397A (en) 1994-03-18 1995-03-20 Taste enhancer

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP21228094A JP3317041B2 (en) 1994-09-06 1994-09-06 New kokumi seasoning material

Publications (2)

Publication Number Publication Date
JPH0870812A true JPH0870812A (en) 1996-03-19
JP3317041B2 JP3317041B2 (en) 2002-08-19

Family

ID=16619994

Family Applications (1)

Application Number Title Priority Date Filing Date
JP21228094A Expired - Lifetime JP3317041B2 (en) 1994-03-18 1994-09-06 New kokumi seasoning material

Country Status (1)

Country Link
JP (1) JP3317041B2 (en)

Also Published As

Publication number Publication date
JP3317041B2 (en) 2002-08-19

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