JPH086492Y2 - Reagent container - Google Patents

Description

[Detailed Description of the Invention] [Industrial field of application] The present invention relates to a reagent container, and more particularly to a reagent container capable of preventing leakage or blowing of a reagent from an outlet.

[Conventional technology]

The measuring reagent based on the immunological reaction principle has high specificity and sensitivity and is used for measuring various substances. Among them, the reagent for agglutination reaction consisting of latex particles sensitized with antigens and antibodies is erythrocyte because it is easy to perform the measurement operation, the result can be obtained in a short time, and immunologically inactive latex particles are used. It is widely used because it has the advantage that it is unlikely to cause non-specific reaction unlike bacteria and the like.

It is convenient to use the agglutination reaction reagent contained in a dropping container. That is, for example, as shown in FIG. 6, a storage part 1, a drip part 2, and a lid part 3 made of polyethylene or the like.
A reagent container consisting of and was used. When this container is used, the reagent is dropped from the nozzle portion 4 by a fixed amount when the storage portion 1 is pushed with the nozzle portion 4 facing downward.

[Problems to be solved by the device]

The drip unit 2 shown in FIG.
Is a columnar body composed of an inner plug portion 5 that closes the open end of and a reagent guide portion 6. Further, in the conventional reagent container, the reagent guide portion 6 has an end surface 7 that is substantially orthogonal to the axis of the columnar body.
Therefore, even when the reagent container was stood upright after use, a small amount of the reagent was likely to adhere to the end surface 7 and remain.

However, reagent containers often need to be refrigerated (for example, stored at 2 to 10 ° C). For example, when a refrigerated reagent container is to be taken out and used, the higher the room temperature, the higher the container temperature. The air inside expands, and when the reagent is dispensed, it blows out and drops more reagent than necessary, or if the reagent container is stood up and left after one use, the air inside the container also expands. Due to the influence of, there was a problem that the reagent was blown out.

The present invention is to solve the above problems in the prior art, and an object of the present invention is to provide a reagent container capable of preventing leakage of reagent during reagent collection and blowing of reagent due to temperature change. is there.

[Means for solving the problem]

That is, the reagent container of the present invention comprises a container (1) capable of containing a reagent therein, a reagent dropping part (2), and a lid part (3). 2) is a plug part (5) for closing the open end of the storage part (1), a nozzle part (4) formed at one end of the plug part (5), and the plug part (5). A columnar body including a reagent guide part (6) formed at the other end, and a through hole (8) for discharging a reagent is formed along the axis thereof, and the reagent guide part (6) is It is characterized by having end faces (7) inclined in the same direction with respect to the axis.

Materials used for the reagent container of the present invention include general-purpose plastics such as polyethylene, polypropylene and polystyrene, metals such as stainless steel, and ceramics, and these may be used alone or in combination. It is practically convenient to use plastic.

The size and shape of the storage section and the lid section are not particularly limited.
Further, the size and shape of the dropping portion can be freely selected within the range where the object of the present invention can be achieved.

The drip portion is composed of an inner plug portion, a nozzle portion, and a reagent guide portion, but these respective portions may be formed separately or may be integrally formed as a whole. It is preferable to integrally form the dropping portion using plastic. The size and shape of the inner plug portion, the nozzle portion, and the reagent guide portion are not limited as long as they are within the scope of the present invention.

The through hole for discharging the reagent is preferably formed in a straight line along the axis of the columnar dropping portion. Further, the shape is such that, when the diameter on the nozzle side is larger and the diameter on the reagent guide side is smaller, the reagent in the through hole easily returns to the storage part after use and the amount of the reagent retained in the through hole is minimized. It is preferable because it can be reduced. Further, if a taper is provided on the inner wall surface of the opening of the through hole of the nozzle portion so that the diameter increases toward the outside, the adhered state of the droplet of the reagent can be stabilized and the dropping accuracy can be improved.

The end surface of the reagent guide portion is inclined at a predetermined angle in the same direction with respect to the axis of the columnar dropping portion. The entire end surface may be formed by one inclined surface, or may be formed by providing a stepped portion such that the vicinity of the opening of the through hole is a plane substantially orthogonal to the axis. . If the end surface is formed of two or more inclined surfaces, each inclined surface may be an inclined surface that is inclined substantially in the same direction. For example, even if the inclined angles differ from each other, the inclined surfaces may be different. Any inclined surface that allows all the droplets adhered to to flow toward one protrusion may be used. If the vicinity of the opening of the through hole is a flat surface, for example, when integrally forming the dropping part using plastic, the pin that is inserted from the nozzle part side to form the through hole does not bend along the slope, and the pin does not bend. This is preferable because it does not cause a trouble such as abrasion on only one side.

[Action]

Since the end surface of the reagent guide portion has an end surface inclined in the same direction with respect to the axis of the columnar dropping portion, the reagent attached to the end surface during use flows along the end surface and gathers at the tip of the reagent guide portion. Drop from here into the storage. For this reason,
It is possible to prevent the above-mentioned inconvenience due to the attachment of the reagent to the end surface.

〔Example〕

The invention will be described in more detail in the following examples.
The following examples are not limiting.

FIG. 1 shows Embodiment 1 of the reagent container of the present invention. In the figure, 1 to 7 have the same meanings as in FIG. In this embodiment, the storage unit 1 is made of polyethylene, the dropping unit 2 is made of polymethylpentene, and the lid unit 3 is made of polypropylene. FIG. 2 shows the dropping part 2 of the reagent container of FIG. Also, the third
The figure is a cross-sectional view taken along the line III-III in FIG. 2, and 8 is a through hole. In the present embodiment, the end surface 7 is a band-shaped flat surface in the vicinity of the opening of the through hole 8 and along a diameter substantially orthogonal to the axis of the drip portion 2.

Embodiment 2 FIG. 4 shows a sectional view of the dropping portion 2 of Embodiment 2 of the present invention. In this embodiment, only the vicinity of the opening of the through hole 8 on the end face 7 is a circular flat surface.

Embodiment 3 FIG. 5 shows a sectional view of the drip unit 2 of Embodiment 3 of the present invention. In this embodiment, the end surface 7 is a flat surface as a whole.

<Performance comparison test> i) Blowing test Two reagent containers for the reagent of Example 1 of the present invention and two conventional reagent containers shown in FIG. 6 were prepared, and a reagent for latex agglutination reaction (milky lotion) ) Was injected in a predetermined amount, and then one drop was dropped, and after a predetermined time, the storage part was pushed to evaluate the state in which droplets or bubbles of the reagent were ejected from the tip of the nozzle part. The results are shown in Table 1. The results are indicated by the following symbols.

 O: No droplets or bubbles of reagent are produced.

 □… Small bubbles of reagent come out.

 Δ: Unstable, with or without bubbles of the reagent.

 ×: Bubbles of the reagent come out.

XX ... About one drop of reagent drops out.

As shown in Table 1 above, when the container of the present invention is pushed immediately after dropping, a small bubble is generated at the tip of the nozzle, but when the pushing force of the container is loosened, the small bubble is sucked inside and collected. . On the other hand, in the conventional product, one drop of liquid overflowed out of the nozzle part, and even if the force pushing the storage part was loosened, it could not be sucked in and collected. Also, after 10 seconds, the product of the present invention generated no bubbles at all, but the conventional product had 20 bubbles.
It was unstable with or without bubbles after 2 seconds.

From the above results, it was found that the product of the present invention is less likely to eject the reagent than the conventional product.

ii) Drop test As in the above i), prepare two containers for the present invention and two containers for conventional reagents, and inject the purified water or the emulsion similar to i) into the storage parts, and then drop each drop. Dropping was performed to evaluate the variation of the dropping amount. The standard value of the purified water in the dropping amount (one drop) is 25 to 30 mg, and the standard value of the emulsion is 23 to 25 mg. The result when the tip of the nozzle is not wiped for each drop
Table 3 shows the results when the tip of the nozzle portion was wiped for each drop.

From the results of Tables 2 and 3 above, the amount of dripping in the product of the present invention is about 2 mg more in purified water than in the conventional product
It was found that the dispersion (R) of the product of the present invention was smaller than that of the conventional product in both purified water and emulsion, although it was almost the same or slightly smaller in emulsion.

[Effect of device]

As described above, in the reagent container of the present invention, since the reagent guide portion has the end surface inclined in the same direction with respect to the axis of the dropping portion, the reagent does not remain attached to the end surface after use or during storage. . Therefore, it is possible to prevent the leakage of the reagent when collecting the reagent and the blowing of the reagent due to the temperature change, the reagent can be effectively used, and the excessive dripping during the test is eliminated, so that the reliability and the working efficiency of the test can be improved. .

[Brief description of drawings]

1 is a front view of a first embodiment of the reagent container of the present invention, FIG. 2 is a front view of a dropping portion of the reagent container of FIG. 1, and FIG. 3 is a line III-III of FIG. FIG. 4 is a sectional view of the dropping part of the reagent container according to the second and third embodiments of the present invention, and FIG. 6 is a front view of an example of a conventional reagent container. In the drawing, 1 ... storage section, 2 ... dripping section, 3 ... lid section, 4 ... nozzle section, 5 ... inner plug section, 6 ... reagent guide section, 7 ... end face, 8 ... through hole

Claims (2)

[Scope of utility model registration request] 1. A storage portion (1) capable of storing a reagent therein,
A reagent container comprising a reagent dropping section (2) and a lid section (3), wherein the dropping section (2) comprises an inner plug section (5) for closing an opening end of the accommodating section (1), A columnar body comprising a nozzle portion (4) formed at one end of the inner stopper portion (5) and a reagent guide portion (6) formed at the other end of the inner stopper portion (5), the axis of which A through hole (8) for discharging a reagent is formed along the container, and the reagent guide portion (6) has an end surface (7) inclined in the same direction with respect to the axis line. . 2. The reagent container according to claim 1, wherein in the end face (7), at least the vicinity of the opening of the through hole (8) is a plane substantially orthogonal to the axis.