JPH0852366A - Partition for petri dish and divided petri dish with partition - Google Patents

Abstract

PURPOSE:To make it unnecessary to dispense a medium into each divided section by communicating a plurality of sections in the divided petri dish main body each on the bottom part without bringing the whole or a part of base sides of partitions into close contact with the bottom face of a petri dish main body. CONSTITUTION:A sheet-like partition 3 provided with a communicating part 5 is prepd. by punching a polystyrene sheet with a thickness of 1mm. The partition 3 is placed in the center of the inside of a petri dish and a culture tool is prepd. by bonding the wall face of the petri dish main body 1 with the vertical part 6 of the partition and the bottom face of the petri dish main body 1 with each leg part 4 of the partition 3 by using an adhesive. When a medium is filled in the culture tool, as the communicating part 5 is provided on the lower part of the wall of the partition for the petri dish, the medium is spread throughout and therefore, trouble for dispensing the medium is reduced and contamination caused by existing bacteria is decreased.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a petri dish partition and a fractionated petri dish with a partition used for culturing microorganisms and the like.

[0002]

2. Description of the Related Art When culturing microorganisms, generally, a sterilized petri dish is poured with a sterilized and dissolved agar-containing medium, left to stand at room temperature, and the cultivated by coating the solid agar medium with the microorganism. For example, it is common to use one petri dish for one microbial sample in a clinical test. However, when many microbial specimens were cultured using a medium composed of the same components, many petri dishes were required accordingly. Therefore, a lot of space for culturing was required. In order to solve the problem, in some cases, a line was drawn on the back of a petri dish using a magic pen or the like, and each of a plurality of microbial specimens was divided and applied with the line as a guide. Alternatively, a petri dish provided with a partition for dividing the interior of the petri dish into a plurality of sections was used.

[0003]

[Problems to be Solved by the Invention] When a plurality of demarcation lines are drawn on the back of a petri dish and a plurality of microorganisms are applied to each demarcation zone, it takes time for motile microorganisms and microorganisms with a fast growth rate. On the agar medium, the tatsutsu had protruded into the adjacent area, and the microorganisms were mixed together. Moreover, when a conventional petri dish with a partition as shown in FIG. 1 that divides the interior of the petri dish into a plurality of compartments is used, when the agar medium is flown, the number of compartments is equal to each of the compartments divided into the two compartments of the petri dish. You just have to dispense
It takes a lot of time and labor, and it takes a long time to pour the medium into one petri dish, and there is a high possibility that the indigenous bacteria will be contaminated. In addition, the partition was sometimes installed when it was in a semi-fluid state immediately before the agar medium was poured and solidified, but the installation timing was difficult, and there was a high possibility of causing contamination of indigenous bacteria.

The present invention has been made in view of the above problems of the prior art, and its main object is to provide a partition for petri dishes or a fractionated petri dish with partitions for solving the above-mentioned drawbacks and inconveniences. And More specifically, it is an object of the present invention to provide a petri dish partition and a fractionated petri dish with a partition that do not require dispensing of a medium into each of the divided compartments.

[0005]

According to the present invention for achieving the above object, the inside of the main body of a petri dish for culturing microorganisms, which is a combination of a dish-shaped main body and a lid covering the main body, is provided in a direction perpendicular to the bottom surface of the main body. In a fraction petri dish divided into a plurality of compartments by the partition, all or part of the bottom side of the partition does not adhere to the bottom surface of the petri dish main body, and the plurality of compartments inside the fraction petri dish main body communicate with each other at the bottom. Thus, when a sterilized fluid medium is poured into only one of the plurality of compartments, it is spread over the other compartments in the same petri dish. That is,
If the culture medium is poured into one section of the Petri dish that has been sectioned,
It can flow to other compartments through a communication part 5 provided in the lower part of the partition 3 as shown in FIG. The fractionated petri dish can be injection-molded, but if it has a communicating portion as in the present invention, a mold for it requires a very high level of technology and cost. Therefore, it is easier to mold only the partition and install it in an existing petri dish to manufacture.

[0006]

Embodiments of the present invention will be described in detail below with reference to FIGS. FIG. 1 is a perspective view of a conventional partitioning petri dish with partitions. FIG. 2 is a perspective view of the partition of the first example of the present invention. FIG. 3 is a cross-sectional view of the partition of Example 1 of the present invention installed in a petri dish. FIG. 4 is a cross-sectional view of the partition of Example 1 of the present invention placed in a petri dish and the medium was poured. Figure 5
8 is a front view of an embodiment of the partition of the present invention. Figure 9
FIG. 3 is a cross-sectional view of the partition of the present invention installed in a petri dish. FIG. 10 is a cross-sectional view of the partition according to Inventive Example 3 installed in a petri dish. FIG. 11 is a perspective view of an embodiment of the partition of the present invention. FIG. 12 is a perspective view of a partition of the second example of the present invention. FIG. 13 is a perspective view of the partition of Example 2 of the present invention installed in a petri dish. FIG. 14 shows Example 4 of the present invention.
It is a perspective view of the partition of.

The petri dish used is generally a so-called 90 mm petri dish (cylindrical dish having a diameter of 90 mm), but any other petri dish such as a 60 mm petri dish or a square petri dish may be used. As the material of the petri dish used, polystyrene is generally used at present, but other materials such as TPX, synthetic resin, and glass can be used. The height of the petri dish partition of the present invention is the same as or lower than the height of the petri dish wall surface, and is higher than the height of the solidified and formed medium, and the communicating portion of the lower part of the partition is higher than the height of the solidified and formed medium. Must be set low. Therefore, it is necessary to set the height corresponding to the size of the petri dish on which the partition is installed. Taking a 90 mm petri dish as an example, the height of the wall surface of the dish main dish 1 is 15 as shown in FIG.
It is about mm, and in general, 20 ml of the medium is often poured in, in which case the height of the medium surface 8 is about 5 mm. The desired effect can be obtained if the height of the partition is 6 mm to 14 mm and the height of the communicating portion 5 below the partition is 1 mm to 4 mm.
However, when the microorganisms are spread on the medium, if the partition is too high, the partition may be an obstacle, so the partition height is more appropriately 6 mm to 10 mm. Therefore, it is necessary to appropriately set the height of the partition and the height of the communicating portion depending on the height and shape of the petri dish of interest. The thickness of the partition wall may be about 1 mm in the case of a normal petri dish. The partition is designed to be in close contact with the wall of the dish-shaped body.

The number of partitions divided by partitions is 90.
In mm petri dish, 2 divisions, 3 divisions, and 4 in 1 petri dish
A section or the like is suitable, and it is possible to provide more sections in a larger square petri dish. The communication part at the bottom of the partition wall must allow the medium to pass smoothly when the medium is poured, and the partition must be stable when the medium is poured, depending on the size of the partition and the shape of the partition. The size and shape of the communication part will change. 5 to 8 are examples of comb-shaped partitions. The shape of the communicating portion may be a straight line as shown in FIG. 5 or a curved upper part as shown in FIG. Further, in order to obtain stability when the medium is poured, one or a plurality of inner leg portions 9 should be provided in addition to the outer end leg portions 4 as shown in FIGS. 7 and 8 depending on the size of the partition. You can also When the partition is installed on the petri dish main body, as shown in FIG. 3, the wall surface of the petri dish dish-shaped body 1 and the vertical partition portion 6 and the bottom surface portion of the petri dish dish-shaped body 1 and the lower end 10 of the partition leg portion are bonded with an adhesive. To do.

FIGS. 9 and 10 show examples of partitions in which the entire bottom of the partition is not in close contact with the dish-shaped body.
FIG. 9 shows that both ends of the partition vertical portion 6 are fixed to the vertical wall portion of the petri dish main body with an adhesive, and the partition bottom 12 is
It is a partition that does not come into close contact with the bottom of the dish-shaped body.
In addition, as shown in FIG. 10, the protrusions 14 are provided at both ends of the upper side of the partition and hooked on the upper end 13 of the vertical wall of the dish-shaped dish-shaped body, and the partition bottom 12 is designed so as not to be in close contact with the bottom of the dish-shaped body. It is a partition. Furthermore, FIG.
1 shows a partition which can be stably installed on the petri dish main body without using an adhesive by increasing the area of the partition installation stabilizing leg lower end 15 in contact with the bottom of the dish. The partition shown in FIG. 14 may be made of polypropylene so that the partition can be stably attached to the petri dish main body without using an adhesive and can be closely attached to the wall of the petri dish main body. Adhesion can be achieved by making the overall length 0.1 to 0.2 mm longer than the inner diameter of the petri dish body. The material of the partition is preferably the same as that of the petri dish such as polystyrene when it is adhered to the inside of the petri dish body using an adhesive. If it is a synthetic resin, the partition is manufactured by pulling out the sheet or injection molding.

(Example 1) A plate having a length of 90 mm, a width of 1 mm, a height of 10 mm, and a communicating portion having a length of 70 mm and a height of 3 mm provided at the bottom by punching out a polystyrene sheet having a thickness of 1 mm as shown in FIG. A partition was prepared. This is a partition that divides a 90 mm diameter petri dish into two. This partition is installed in the center of the inside of a petri dish having a diameter of 90 mm, and an adhesive is used to form the wall surface of the dish-shaped body 1 and the vertical partition 6 and the bottom of the dish-shaped main body 1 and each leg 4 of the partition as shown in FIG. Bottom edge 10
A desired effect could be obtained by using an incubator prepared by adhering and fixing the above with an adhesive and filling with a medium as shown in FIG.

(Example 2) Polystyrene is injection-molded and combined with a plate having a communication part having a length of 45 mm, a width of 1 mm, a height of 10 mm, a length of 35 mm and a height of 3 mm at a central angle of 120 °. A Y-shaped partition as shown in FIG. 12 was produced. This is a partition that divides a 90 mm diameter petri dish into three parts. When this partition was installed and used in the same manner as in Example 1 inside a petri dish having a diameter of 90 mm as shown in FIG. 13, a desired effect could be obtained.

(Example 3) Polystyrene was injection-molded and
A partition having protrusions on both ends of the partition 3 shown in FIG. When the projection 14 is applied to the upper end of the 90 mm petri dish main body wall, the distance between the partition bottom 12 and the petri dish main body bottom is 3 mm. When the medium was poured into this and used, the desired effect could be obtained.

Example 4 Polypropylene was injection-molded to form partitions as shown in FIG. A portion having a communication portion at the center is 70 mm, and a length (A in FIG. 14) from two centers of one forked tip 17 to the other is 90.
It was set to 2 mm. When this was forcibly pushed into the inside of a 90 mm petri dish, the tips of the forks were slightly close to each other, and they were adhered to the inner wall of the petri dish body.
At this time, the height of the communicating portion from the bottom of the petri dish body was 3 mm. When the medium was poured into this and used, the desired effect could be obtained.

[0014]

As described above in detail, since the lower portion of the wall of the partition for petri dishes of the present invention is provided with the communicating portion, if the medium is poured into one compartment divided by the partition, the medium is spread out. It became so. Therefore, the labor of dispensing the medium was reduced, and the contamination of indigenous bacteria during the operation was also reduced.

[0015]

[Brief description of drawings]

FIG. 1 is a perspective view of a conventional fractionated petri dish with partitions.

FIG. 2 is a perspective view of a partition according to Example 1 of the present invention.

FIG. 3 is a cross-sectional view of the partition of Example 1 of the present invention installed in a petri dish.

FIG. 4 is a cross-sectional view of the partition according to Example 1 of the present invention placed in a petri dish and the medium flowed.

FIG. 5 is a front view of an embodiment of the partition of the present invention.

FIG. 6 is a front view of an embodiment of the partition of the present invention.

FIG. 7 is a front view of an embodiment of the partition of the present invention.

FIG. 8 is a front view of an embodiment of the partition of the present invention.

FIG. 9 is a cross-sectional view of an example in which the partition of the present invention is installed in a petri dish.

FIG. 10 is a cross-sectional view of a partition according to Inventive Example 3 installed on a petri dish.

FIG. 11 is a perspective view of an embodiment of the partition of the present invention.

FIG. 12 is a perspective view of a partition according to Example 2 of the present invention.

FIG. 13 is a perspective view of the partition according to Inventive Example 2 installed on a petri dish.

FIG. 14 is a perspective view of a partition according to Example 4 of the present invention.

[Explanation of symbols]

 1 Petri dish main body 2 Partition of conventional petri dish with partition 3 Partition 4 Partition outer end leg 5 Partition communicating part 6 Vertical partition 7 Petri dish lid 8 Medium surface 9 Partition inner leg 10 Partition leg lower end 11 Petri dish Main body vertical wall 12 Partition bottom 13 Petri dish Main body vertical wall Upper end 14 Projection 15 Partition installation stabilizing leg Lower end 16 Two-way 17 Two-way tip

Claims (4)

[Claims] 1. A fractionated petri dish divided into a plurality of compartments by a partition provided inside the main body of a petri dish for culturing microorganisms, which is a combination of a dish-shaped main body and a lid covering the main body, in a direction perpendicular to the bottom surface of the main body. 2. The partitioning petri dish with partitions, wherein all or part of the bottom of the partition does not adhere to the bottom surface of the petri dish main body, and a plurality of compartments inside the fractionation petri dish main body communicate with each other at the bottom. 2. A petri dish with a partition according to claim 1,
Inside the body of the petri dish that is not fractionated by the partition, all or part of the bottom of the partition does not adhere to the bottom of the petri dish body, and a plurality of compartments inside the fractionated petri dish body can communicate with each other at the bottom. A petri dish partition that can be manufactured by installing it vertically to the bottom surface of the main body. 3. The partition for a petri dish according to claim 2, wherein a protrusion protruding from the inner diameter of the petri dish main body for hooking on the upper side of the vertical wall of the petri dish main body is provided at the end of the upper side. 4. The petri dish main body, wherein the portion in contact with each wall is branched into two portions.
Partition for petri dish described.