JPH084589Y2 - Injection device for creating calibration curve - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION (Industrial field of application) The present invention relates to an injection device used for measuring the content of a specific component contained in a solid sample by a calibration curve.

(Prior Art) For example, a mixed feed for animals may contain various additives and medicines for various purposes (for example, prevention of diseases). However, the content of a specific component among them may be accurately controlled. There is something that must be done.

Conventionally, a measurement method using a calibration curve is known as one of the methods for measuring the content of a specific component in such a solid sample. In this method, first, a component to be measured is extracted from a solid sample having a known content with a chemical, the extract is adsorbed on a specific adsorbent, and then another chemical is used to remove the adsorbent. The target component is eluted and the eluate is irradiated with light of a specific wavelength to measure its absorbance.
By making similar measurements for known contents of ~ 3, a straight line showing the relationship between the absorbance and the contents (this straight line is referred to as a calibration curve) is created, and then similar measurements are made for solid samples of unknown contents. Is finally obtained as the absorbance, and the content of that component is measured using the calibration curve already obtained.

By the way, depending on the components to be measured, if the production conditions of the solid sample or the conditions of the series of measurement operations are subtly changed, the content determined by the calibration curve will change because the color development of the eluate will be greatly affected. It is difficult to perform accurate analysis and requires skill in operation. Even an expert takes time and effort to measure,
During that time, there are many operations that cannot be released, and even when the same feed is analyzed, the results often differ from day to day and from person to person. Therefore, for example, in the case of mixed feed, it is necessary to create a calibration curve each time it is measured for each feed brand.

In the content measurement method using the calibration curve, it takes such time and labor to make the calibration curve, and the delicate and complicated operation must be repeated several times (at least about three times) for the known and unknown content. The problem is that it is very annoying.

(Object and structure of the invention) The present invention has been made in view of the above points, and aims to simplify the measurement operation for creating the calibration curve and shorten the time required. , A plurality of press type injectors containing unknown sample liquid to be measured and known extract liquid are arranged side by side, and the reciprocating motion member acts on the head of each injector to push out the extract liquid inside the injector, and the adsorbent It is configured so that a reaction cartridge containing

(Example) Hereinafter, the present invention will be described with reference to the drawings.

FIG. 1 is a perspective view of an injection device according to the present invention.

The injection device according to the present invention is a device used for injecting an extraction liquid extracted from a solid sample to be measured in an adsorbent cartridge into an adsorbent cartridge in a content measuring method using a calibration curve, The example shown is of the type that uses four injectors.

In the figure, two support frames 2 and 3 are mounted on a horizontal base 1.
Is erected vertically, and a horizontal support plate 21 is fixed in the middle of the support frame 2. The support plate 21 is provided with four holes 21a through which the injectors J pass loosely. Also,
On the upper part of the support frame 2, grippers 22a, 22b, 22c, 22d having a structure for loosely gripping the injector J are fixed. The injector J has the same structure as the injector, and is supported by the flange of the outer tube J ₁ thereof engaging with the upper surfaces of the grippers 22a to 22d.

On the other hand, the upper part of the other support frame 3 is bent horizontally, and a motor M and a housing 4 having a conversion mechanism for converting the rotation of the motor into a straight vertical movement are installed in the horizontal portion 3a. A rack 5 extends downward from a horizontal portion 3a of the support frame 3, and one side of the rack 5 has a tooth 5a which meshes with a pinion (not shown) in the housing 4.

A horizontal pressing plate 6 is fixed to the lower end of the rack 5,
Heads 6a to 6d are provided on the lower surface of the horizontal pressing plate 6 at equal positions at the positions of the injectors J. The distance between each head 6a and the horizontal pressing plate 6 can be finely adjusted with a screw.

The horizontal pressing plate 6 has a rear part bent vertically downward,
The receiving portion 6e of the guide rod 7 is fixed to this vertical portion. When the horizontal pressing plate 6 moves up and down, the receiving portion 6e slidably receives the guide rod 7 extending downward from the arm 3b fixed to the upper portion of the support frame 3 and fulfills the guide function. The guide rod 7 and the receiving portion 6e are provided one each on the left and right.

Next, a work procedure and an operation of the apparatus when the extract is adsorbed on the adsorbent using the injection apparatus will be described.

Prior to this operation, a predetermined chemical is added to the same amount of solid sample having three different known contents (for example, 45 mg, 60 mg, and 75 mg) for each component to be measured to extract the components to be measured. The same amount of each of these three kinds of extract liquid is put into each of the three injectors J, and the same extract liquid of a solid sample having an unknown content to be measured is put into the remaining injectors J.

In this way, the injectors J containing the known and unknown solid sample extraction liquids with different contents are respectively attached to the grippers 22a to 22d.
Supported through, to lightly abut against each head 6a~6d to the head of the inner tube rear inner tube J ₂ inserting the J _2.

On the other hand, at the lower end of the outer pipe J ₁ of each injector J, a cartridge K filled with an adsorbent is engaged in a liquid-tight state from below, as shown in FIG.
Beakers B _{a to} B _d are placed under each.

When an operation switch (not shown) is turned on, the motor M
Rotates, and the rack 5 starts slowly moving downward through the rack and pinion conversion mechanism. As a result, the horizontal pressing plate 6
Is guided by the guide rod 7 and descends while maintaining the horizontal state, and the heads of the inner pipes J ₂ of the four injectors J are moved to the heads 6a to 6a.
It is pushed down via 6d.

As a result, the extract liquids in the four injectors J are simultaneously injected into the cartridge K and adsorbed by the adsorbent therein. Extracts were passed through the cartridge K is accumulated in a beaker B _a .about.B _d below.

The rack 5 is lowered and the inner pipe J ₂ of the injector J is replaced with the outer pipe J ₁
The adsorption process ends when the rack 5 is lowered to the deepest position and the rack 5 is raised.

Next, the injector J is changed to one containing washing water, and the same operation is performed once to wash the adsorbent in the cartridge K with water. After that, similar washing is performed with water and a predetermined chemical (for example, methanol). Finally, the injector J containing a predetermined chemical (for example, methanol) and water is installed on the horizontal support plate 21.
Also, the rack 5 is supported by the grippers 22a to 22d, and the rack 5 is lowered again to inject a mixed liquid of a predetermined chemical and water into the cartridge K to elute the target component adsorbed by the adsorbent. During this time, the beaker is changed each time, so the final eluate is collected in the beaker.

The eluates with different contents collected in the four beakers are subjected to a series of treatments to measure the absorbance and used to prepare a calibration curve. The treatments and procedures for this are the same as those conventionally performed. However, since it is not the gist of the present invention, its description is omitted here.

In this way, using the injector device eliminates the troublesome work of changing the injection liquid for the four injectors and repeating the same operation several times, and only the simple work of replacing the injectors is required. .

The present invention can be used for component analysis by measuring the content of a solid sample in general by a calibration curve.

(Effect of the Invention) As described above, in the present invention, the press type injectors of the unknown sample liquid and the plurality of known sample liquids are arranged side by side, and the reciprocating motion member acts on the head of each injector. Since the extraction liquid inside the injector is pushed out, multiple injectors can be operated at the same time with a single operation, which simplifies part of the time-consuming and time-consuming calibration curve creation work, and shortens the time. Become. Further, since a plurality of injectors are simultaneously operated by the same actuating member, the extraction speed of the extract is also the same, which is effective in reducing the dispersion of the measurement results.

[Brief description of drawings]

FIG. 1 is a perspective view of an embodiment of the injection device according to the present invention, and FIG. 2 is a side view of the injection device shown in FIG. 1 ... support stand, 2,3 ... support frame, 22a to 22d ... Hajiko, J ... injector, J ₁ ... outer tube, J ₂ ... inner tube, 6 a to 6 d ...
Head, 5 ... rack

Claims (1)

[Scope of utility model registration request] 1. An injector support means for supporting one injector containing a sample liquid to be measured and a plurality of pressing type injectors containing a sample liquid of a known concentration side by side, and acting simultaneously on the heads of the respective injectors. An injector for producing a calibration curve, comprising: an actuating member that reciprocates so as to push out the extract inside the injector to the outside of the injector; and a reaction cartridge attached to the tip of the injector.