JPH0838159A - Photosynthetic culturing apparatus by culture liquid film forming system - Google Patents
Photosynthetic culturing apparatus by culture liquid film forming systemInfo
- Publication number
- JPH0838159A JPH0838159A JP6183776A JP18377694A JPH0838159A JP H0838159 A JPH0838159 A JP H0838159A JP 6183776 A JP6183776 A JP 6183776A JP 18377694 A JP18377694 A JP 18377694A JP H0838159 A JPH0838159 A JP H0838159A
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- Prior art keywords
- culture
- gas
- upper wall
- dome
- culture solution
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M31/00—Means for providing, directing, scattering or concentrating light
- C12M31/02—Means for providing, directing, scattering or concentrating light located outside the reactor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/02—Photobioreactors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
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- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Sustainable Development (AREA)
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- Microbiology (AREA)
- Biomedical Technology (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Thermal Sciences (AREA)
- Analytical Chemistry (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Treating Waste Gases (AREA)
- Carbon And Carbon Compounds (AREA)
Abstract
Description
【０００１】[0001]
【産業上の利用分野】本発明は、培養液膜形成方式によ
る光合成培養装置に係り、特に光反応を主として行う反
応系、例えばクロレラ、スピルリナ等の微細藻類や植物
細胞を培養する装置であって、光照射効率が高く、菌体
の培養到達濃度を向上させることができる培養液膜形成
方式による光合成培養装置に関するものである。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a photosynthetic culture device using a culture solution film formation system, and more particularly to a reaction system for mainly performing a photoreaction, for example, a device for culturing microalgae such as chlorella and spirulina or plant cells. The present invention relates to a photosynthetic culturing device by a culture solution film forming system, which has a high light irradiation efficiency and can improve the concentration of cells that reach culture.
【０００２】[0002]
【従来の技術】従来の光合成培養装置として、例えば特
開昭５２−１０８０８２号公報に記載された、光源を内
臓した容器の内壁面に培養液を薄膜で流下させるものが
あげられる。図５は、このような光合成培養装置を示す
説明図である。この装置は、ドーム型の上壁面５１を有
する中空容器５２と、該中空容器５２の下部に設けられ
た培養槽５３と、該培養槽内に設けられた複数の、例え
ばスパージャ等からなる散気管５７と、培養槽５３を貫
通するように前記中空容器５２内に設けられた複数の光
源５４と、培養槽５３の中央部に設けられた、培養液を
噴出する噴出ノズル５６と、該噴出ノズル５６に連結さ
れた循環ポンプ５５とから主として構成されている。2. Description of the Related Art As a conventional photosynthetic culture apparatus, there is, for example, one described in Japanese Patent Application Laid-Open No. 52-108082, which causes a culture solution to flow down in a thin film on the inner wall surface of a container containing a light source. FIG. 5 is an explanatory view showing such a photosynthetic culture device. This device includes a hollow container 52 having a dome-shaped upper wall surface 51, a culture tank 53 provided in the lower portion of the hollow container 52, and a plurality of, for example, sparger and the like diffuser tubes provided in the culture tank. 57, a plurality of light sources 54 provided in the hollow container 52 so as to penetrate the culture tank 53, a jet nozzle 56 for jetting a culture solution, which is provided in the central portion of the culture tank 53, and the jet nozzle. It is mainly composed of a circulation pump 55 connected to 56.
【０００３】散気管５７から培養液５８中に噴射され
る、例えば二酸化炭素（以下、炭酸ガスまたはＣＯ2 と
いう）が溶解した培養液５８は循環ポンプ５５を経て噴
出ノズル５６から上方に向かって噴出され、中空容器５
２のドーム型上壁面５１の内面に衝突し、培養液薄膜５
９を形成しつつ、前記培養槽５３へ流下して循環する。
このとき、培養液５８に懸濁した菌体は、培養液に溶存
した前記ＣＯ2 の存在下、光源５４から照射される照射
光を受けて増殖する。A culture solution 58, for example, in which carbon dioxide (hereinafter referred to as carbon dioxide gas or CO 2 ) is dissolved, is jetted from the air diffuser 57 into the culture solution 58, and is jetted upward from a jet nozzle 56 via a circulation pump 55. And the hollow container 5
2 colliding with the inner surface of the dome-shaped upper wall surface 51, the culture solution thin film 5
9 is formed and is circulated by flowing down to the culture tank 53.
At this time, the bacterial cells suspended in the culture medium 58 grow by receiving the irradiation light emitted from the light source 54 in the presence of the CO 2 dissolved in the culture medium.
【０００４】しかしながら、上記従来技術は、ＣＯ2 の
通気手段として散気管方式のものを使用しているため
に、気液接触効率が十分でなく、炭素（Ｃ）源の供給律
速阻害が発生し易いという問題がある。また培養液５８
の循環手段として循環ポンプを使用しているために、物
理的な衝撃によって菌体が破壊され易いという問題もあ
る。However, in the above-mentioned prior art, since the diffuser tube system is used as the CO 2 aeration means, the gas-liquid contact efficiency is not sufficient and the rate-limiting inhibition of the carbon (C) source supply occurs. There is a problem that it is easy. In addition, the culture medium 58
Since a circulation pump is used as the circulation means, there is a problem that the bacterial cells are easily destroyed by physical impact.
【０００５】[0005]
【発明が解決しようとする課題】本発明の目的は、上記
従来技術の問題点を解決し、気液接触効率が高く、しか
も菌体に必要以上の物理的な衝撃を与えることがなく、
ドーム型上壁面の内面に液膜を形成させ、菌体の生育到
達濃度を向上させることができる培養液膜形成方式によ
る光合成培養装置を提供することにある。SUMMARY OF THE INVENTION An object of the present invention is to solve the above-mentioned problems of the prior art, to provide a high gas-liquid contact efficiency, and to prevent undesired physical impact on the cells.
It is an object of the present invention to provide a photosynthetic culture device by a culture liquid film forming method capable of forming a liquid film on the inner surface of a dome-shaped upper wall surface and improving the growth reaching concentration of cells.
【０００６】[0006]
【課題を解決するための手段】上記目的を達成するた
め、本願で特許請求する発明は以下のとおりである。 （１）透明なドーム型の上壁面を有する中空容器と、該
中空容器の下部を形成する培養槽と、該培養槽に設けら
れた培養液の温度制御手段と、前記培養槽の中心部に設
けられ、前記ドーム型の上壁面に向かって培養液を噴出
する気液混合エジェクターと、該気液混合エジェクター
に連結された炭素含有ガスの供給源と、前記ドーム型上
壁面の上部または下部空間部に設けられた光源を有する
ことを特徴とする培養液膜形成方式による光合成培養装
置。In order to achieve the above object, the invention claimed in the present application is as follows. (1) A hollow container having a transparent dome-shaped upper wall surface, a culture tank forming a lower portion of the hollow container, a temperature control means for a culture solution provided in the culture tank, and a central portion of the culture tank. A gas-liquid mixing ejector, which is provided and ejects a culture solution toward the dome-shaped upper wall surface, a carbon-containing gas supply source connected to the gas-liquid mixing ejector, and an upper or lower space of the dome-shaped upper wall surface. A photosynthetic culture device by a culture solution film forming method, characterized in that it has a light source provided in its part.
【０００７】[0007]
【作用】培養液を噴出する手段として気液混合エジェク
ターを用いたことにより、エジェクターに供給される炭
素含有ガスと培養液との接触効率が向上するので、培養
液中に十分な炭素が供給されるとともに、機械的な攪拌
による菌体の破壊またはストレスの発生が防止される。[Function] By using the gas-liquid mixing ejector as a means for ejecting the culture solution, the contact efficiency between the carbon-containing gas supplied to the ejector and the culture solution is improved, so that sufficient carbon is supplied to the culture solution. In addition, the destruction of the bacterial cells or the occurrence of stress due to mechanical agitation is prevented.
【０００８】また、中空容器の上壁面を透明なドーム型
とし、中空容器内下方に温度制御手段を有する培養槽を
設け、前記上壁面の上部または下部空間部に光源を配置
したことにより、培養槽の中心部に設けられた前記気液
混合エジェクターによって、炭素を含んだ培養液を前記
ドーム型の上壁面に向かって噴出すると、噴出された培
養液は前記ドーム型の上壁面に衝突して内面に薄膜を形
成しながらその内壁面に沿って流下する。このとき、培
養液は薄膜状で光源からの光を受けるので、培養液に懸
濁した菌体は、その濃度が高くても十分な光を受けて効
率的に増殖する。In addition, by arranging a transparent dome shape on the upper wall surface of the hollow container, providing a culture tank having a temperature control means in the lower part of the hollow container, and disposing a light source in the upper or lower space of the upper wall surface, By the gas-liquid mixing ejector provided in the center of the tank, when the culture solution containing carbon is ejected toward the upper wall surface of the dome, the ejected culture solution collides with the upper wall surface of the dome. While forming a thin film on the inner surface, it flows down along the inner wall surface. At this time, since the culture solution is in the form of a thin film and receives light from the light source, the bacterial cells suspended in the culture solution receive sufficient light even if the concentration thereof is high and efficiently proliferate.
【０００９】光合成反応は、光照射量が制限基質因子と
なるので、菌体の育成を阻害しない範囲内で十分な照射
量を確保することが必要となる。菌体濃度が高くなって
も高い照射効率を維持することができる、本発明の薄膜
を形成して光を照射する方法は高濃度培養に最も適して
いる。本発明において、ドーム型の上壁面は、例えばガ
ラス、アクリル樹脂、塩化ビニル等の透明の材質で構成
される。培養槽は、ジャケット構造のものが好ましく、
培養槽に設けられる培養液の温度制御手段としては、例
えば冷水または温水を循環する循環式のものが好適に使
用される。また、培養槽には、培養液中の溶存酸素濃
度、溶存ＣＯ2 濃度、培養液の温度等を検出する反応モ
ニターを設けることが好ましい。In the photosynthetic reaction, the light irradiation amount becomes a limiting substrate factor, so that it is necessary to secure a sufficient irradiation amount within the range that does not inhibit the growth of the bacterial cells. The method of forming a thin film and irradiating with light of the present invention, which can maintain high irradiation efficiency even when the cell concentration is high, is most suitable for high-concentration culture. In the present invention, the dome-shaped upper wall surface is made of a transparent material such as glass, acrylic resin, or vinyl chloride. The culture tank preferably has a jacket structure,
As a temperature control means for the culture solution provided in the culture tank, for example, a circulation type device for circulating cold water or hot water is preferably used. Further, the culture tank is preferably provided with a reaction monitor for detecting the dissolved oxygen concentration, the dissolved CO 2 concentration, the temperature of the culture liquid, etc. in the culture liquid.
【００１０】本発明において、培養液をドーム型の上壁
面に向けて噴出する気液混合エジェクターは、例えば、
培養槽の中央に配置され、その先端部には前記ドーム型
上壁面に向かって開口する拡径部を有する案内管が連結
される。また、気液混合エジェクターの気体噴出ノズル
は、配管を介して、例えば炭素源としてＣＯ2 を供給す
るＣＯ2 ボンベと連結されている。In the present invention, the gas-liquid mixing ejector for ejecting the culture solution toward the dome-shaped upper wall surface is, for example,
A guide tube is arranged at the center of the culture tank, and a tip end portion thereof is connected to a guide tube having an enlarged diameter portion opening toward the dome-shaped upper wall surface. Moreover, the gas jet nozzle of the gas-liquid mixing ejector, through a pipe, for example, is connected to the CO 2 cylinder for supplying CO 2 as a carbon source.
【００１１】本発明において、ドーム型の上壁面の上部
または下部空間部に設けられる、光合成用の光源として
は、例えば人工光源としてハロゲンランプ、螢光ラン
プ、白熱ランプ等が、また自然光源としては太陽光が用
いられる。In the present invention, the light source for photosynthesis provided in the upper or lower space of the dome-shaped upper wall is, for example, a halogen lamp, a fluorescent lamp, an incandescent lamp or the like as an artificial light source, and a natural light source. Sunlight is used.
【００１２】[0012]
【実施例】次に、本発明を実施例によってさらに詳細に
説明する。図１は、本発明の一実施例を示す光合成培養
装置の説明図、図２は、図１の噴出ノズルの外形図、図
３は図２の III−III 線矢示方向断面図である。図１に
おいてこの培養装置は、透明な材質、例えばガラスから
なるドーム型の上壁面１を有する中空容器２と、該中空
容器２の下部に設けられたジャケット構造の培養槽３
と、該培養槽３の温度制御手段である循環式恒温槽４
と、前記培養槽３の中心部に設けられた、前記ドーム型
上壁面１に向かって培養液１７を噴出する気液混合エジ
ェクター６と、該気液混合エジェクター６に光合成反応
ガスライン１０を介して連結された炭素供給源としての
ＣＯ2 ボンベ１１と、前記ドーム型上壁面１の上部空間
部に設けられた光源としてのハロゲンランプ８とから主
として構成されている。なお、９はコンプレッサー、１
２および１３はフローメータ、１６は培養液の溶存酸素
濃度、溶存ＣＯ2 濃度または／および培養液温度を検出
するセンサーである。また、ＣＯ2 ボンベ１１にはＣＯ
2 の流出量を制御するレギュレータが設けられている。EXAMPLES Next, the present invention will be described in more detail by way of examples. 1 is an explanatory view of a photosynthetic culture apparatus showing an embodiment of the present invention, FIG. 2 is an outline view of the ejection nozzle of FIG. 1, and FIG. 3 is a sectional view taken along the line III-III of FIG. In FIG. 1, this culture apparatus comprises a hollow container 2 having a dome-shaped upper wall surface 1 made of a transparent material such as glass, and a culture tank 3 having a jacket structure provided below the hollow container 2.
And a circulation type constant temperature bath 4 which is a temperature control means of the culture bath 3.
And a gas-liquid mixing ejector 6 for ejecting a culture solution 17 toward the dome-shaped upper wall surface 1 provided in the center of the culture tank 3, and a photosynthetic reaction gas line 10 to the gas-liquid mixing ejector 6. A CO 2 cylinder 11 as a carbon supply source and a halogen lamp 8 as a light source provided in the upper space of the dome-shaped upper wall 1 are mainly connected. In addition, 9 is a compressor, 1
Reference numerals 2 and 13 are flow meters, and 16 is a sensor for detecting the dissolved oxygen concentration, the dissolved CO 2 concentration or / and the culture solution temperature of the culture solution. Also, the CO 2 cylinder 11 has CO
A regulator for controlling the outflow amount of 2 is provided.
【００１３】図２および３において、前記気液混合エジ
ェクター６は、液体流入孔２１を有する外管２２と、該
外管２２の下部閉塞端２３に連結された気体噴出ノズル
２４とからなり、該気体噴出ノズル２４の先端部２８は
前記外管２２の液体流入孔２１の上端部よりも所定の長
さ、例えば５ｍｍだけ上方に延びている。エジェクター
６の外管２２の上部開口部２５には、拡径した上部開口
端２６を有する案内管２７が連結されている。2 and 3, the gas-liquid mixing ejector 6 comprises an outer tube 22 having a liquid inflow hole 21, and a gas ejection nozzle 24 connected to a lower closed end 23 of the outer tube 22. The tip portion 28 of the gas ejection nozzle 24 extends upward by a predetermined length, for example, 5 mm, from the upper end portion of the liquid inflow hole 21 of the outer tube 22. A guide tube 27 having an enlarged upper opening end 26 is connected to the upper opening 25 of the outer tube 22 of the ejector 6.
【００１４】このような構成において、ＣＯ2 ボンベ１
１に充填されたＣＯ2 はコンプレッサ９から押し出され
る空気流に伴って光合成反応ガスライン１０を経てエジ
ェクター６の気体噴出ノズル２４に流入し、外管２２の
液体流入孔２１から流入する、循環式恒温槽４によっ
て、例えば２５℃に温度調節された培養液１７と接触混
合し、該培養液１７に溶解する。ＣＯ2 が溶解した培養
液１７は、気液混合エジェクター６に流入する前記ＣＯ
2 を含んだ空気流のガスリフト効果によって押し上げら
れ、その先端部に連結された案内管２７の拡径した上部
開口端２６から上方に向かって噴出され、ドーム型上壁
面１に衝突し、その内壁面に沿って薄膜１８を形成しな
がら流下する。このとき培養液の薄膜１８内に懸濁した
菌体は、該培養液に溶存する前記ＣＯ2 の存在下で、ハ
ロゲンランプ８の照射光を受けて増殖する。増殖した菌
体を含む培養液の薄膜１８は、ドーム型上壁面１の内壁
面に沿って流下して培養槽３に戻され、以下同様にして
中空容器２内を循環し、菌体の培養が行われる。培養槽
３内の培養液１７に溶存するＣＯ2 濃度、酸素濃度、お
よび培養液温度はそれぞれセンサー１６によって監視さ
れ、菌体の育成に最適な条件に調整される。In such a structure, the CO 2 cylinder 1
The CO 2 filled in 1 flows into the gas ejection nozzle 24 of the ejector 6 through the photosynthetic reaction gas line 10 along with the air flow pushed out from the compressor 9, and then flows in from the liquid inflow hole 21 of the outer pipe 22. In the constant temperature bath 4, for example, the mixture is brought into contact with the culture solution 17 whose temperature is adjusted to 25 ° C. and dissolved in the culture solution 17. The culture solution 17 in which CO 2 is dissolved flows into the gas-liquid mixing ejector 6
It is pushed up by the gas lift effect of the air flow containing 2 and is ejected upward from the expanded upper opening end 26 of the guide tube 27 connected to the tip end thereof, colliding with the dome-shaped upper wall surface 1, and It flows down while forming the thin film 18 along the wall surface. At this time, the bacterial cells suspended in the thin film 18 of the culture solution grow under the irradiation of the halogen lamp 8 in the presence of the CO 2 dissolved in the culture solution. The thin film 18 of the culture solution containing the grown bacterial cells flows down along the inner wall surface of the dome-shaped upper wall surface 1 and is returned to the culture tank 3, and thereafter circulates in the hollow container 2 in the same manner to culture the bacterial cells. Is done. The concentration of CO 2 dissolved in the culture solution 17 in the culture tank 3, the oxygen concentration, and the temperature of the culture solution are monitored by the sensor 16 and adjusted to the optimum conditions for growing the bacterial cells.
【００１５】本実施例によれば、ＣＯ2 を含んだ空気流
を利用して気液混合エジェクター６のガスリフト効果に
よる推進力によって培養液１７を上方に向かって噴出す
ようにしたことにより、気液接触効率が向上して培養液
１７へのＣＯ2 の溶解が促進するとともに、培養液に必
要以上の物理的な力を作用させることがないので、菌体
のストレスを防止してその増殖作用を促進することがで
きる。According to this embodiment, the culture fluid 17 is jetted upward by the propulsive force of the gas-liquid mixing ejector 6 by the gas lift effect using the air flow containing CO 2. The liquid contact efficiency is improved, the dissolution of CO 2 in the culture solution 17 is promoted, and the culture solution is not subjected to an excessive physical force. Can be promoted.
【００１６】また、ドーム型上壁面の内側に培養液１の
薄膜１８を形成するこができるので、高濃度培養液であ
っても十分な光照射効率を確保して培養到達濃度を高め
ることができる。次に、本発明の具体的実施例を説明す
る。Further, since the thin film 18 of the culture solution 1 can be formed on the inner side of the dome-shaped upper wall surface, it is possible to secure sufficient light irradiation efficiency and increase the culture arrival concentration even with a high concentration culture solution. it can. Next, specific examples of the present invention will be described.
【００１７】実施例１ 図１の装置において培養液として改変デトマーを用い、
培養液温度を２５℃、通気量を１ｖｖｍ（１％ＣＯ2 混
合空気通気量６リットル／ｍｉｎ、培養液循環量：１．
０リットル／ｍｉｎ）、照射量を４５０μＥ／ｍ2・Ｓ
として、初期菌体濃度０．０５ｇ／リットルのアナベナ
の培養試験を行ったところ、培養開始１０日後の培養到
達濃度は約１．５ｇ／リットルであった。Example 1 In the apparatus of FIG. 1, a modified detomer was used as a culture solution,
The culture solution temperature was 25 ° C., the aeration rate was 1 vvm (1% CO 2 mixed air aeration rate 6 liter / min, culture solution circulation rate: 1.
0 liter / min), irradiation dose 450 μE / m 2 · S
As a result, when an anabaena culture test was carried out with an initial cell concentration of 0.05 g / liter, the reached concentration after 10 days from the start of culture was about 1.5 g / liter.
【００１８】比較例１ 図５に示した従来装置を用い、上記実施例１と同様の条
件で同様の培養テストを行ったところ、培養開始１０日
後の培養到達濃度は約１．０ｇ／リットルであった。実
施例１および比較例１の結果を図４に示す。図におい
て、本発明の光合成培養装置を用いた実施例１は、比較
例１に比べて培養到達速度が著しく向上したことが分か
る。Comparative Example 1 Using the conventional apparatus shown in FIG. 5, the same culture test was carried out under the same conditions as in Example 1 above. As a result, the concentration reached after 10 days from the start of culture was about 1.0 g / liter. there were. The results of Example 1 and Comparative Example 1 are shown in FIG. In the figure, it can be seen that in Example 1 using the photosynthetic culture device of the present invention, the arrival rate of culture was significantly improved as compared with Comparative Example 1.
【００１９】[0019]
【発明の効果】本発明によれば、培養液の噴出手段とし
て炭素含有ガスと培養液とを混合して上方に噴出する気
液混合エジェクターを用いたことにより、気液接触効率
が向上し、しかも菌体に必要以上の物理的な衝撃力およ
びストレスを与えることがないので、菌体の培養到達速
度が著しく向上する。According to the present invention, the gas-liquid contact efficiency is improved by using the gas-liquid mixing ejector that mixes the carbon-containing gas and the culture solution and ejects them upward as the means for ejecting the culture solution. Moreover, since the cells are not subjected to unnecessary physical impact force and stress, the cell arrival rate is significantly improved.
【図１】本発明の一実施例を示す光合成培養装置の説明
図。FIG. 1 is an explanatory view of a photosynthetic culture device showing one embodiment of the present invention.
【図２】図１の気液混合エジェクターを示す外形図。FIG. 2 is an outline view showing the gas-liquid mixing ejector of FIG.
【図３】図２の III−III 線矢示方向縦断面図。FIG. 3 is a vertical cross-sectional view taken along line III-III of FIG.
【図４】本発明の効果を示す説明図。FIG. 4 is an explanatory view showing the effect of the present invention.
【図５】従来技術の説明図。FIG. 5 is an explanatory diagram of a conventional technique.
１…ドーム型上壁面、２…中空容器、３…培養槽、４…
循環式恒温槽、６…気液混合エジェクター、８…ハロゲ
ンランプ、９…コンプレッサー、１０…光合成反応用ガ
スライン、１１…ＣＯ2 ボンベ、１６…センサー、１７
…培養液、１８…培養液の薄膜、２１…液体流入孔、２
２…外管、２３…外管の閉塞端、２４…気体噴出ノズ
ル、２５…上部開口部、２６…拡径した上部開口端、２
７…案内管、２８…気体噴出ノズルの先端部。1 ... Dome-shaped upper wall surface, 2 ... Hollow container, 3 ... Culture tank, 4 ...
Circulation type constant temperature bath, 6 ... Gas-liquid mixing ejector, 8 ... Halogen lamp, 9 ... Compressor, 10 ... Gas line for photosynthesis reaction, 11 ... CO 2 cylinder, 16 ... Sensor, 17
... culture liquid, 18 ... culture liquid thin film, 21 ... liquid inflow hole, 2
2 ... outer pipe, 23 ... closed end of outer pipe, 24 ... gas ejection nozzle, 25 ... upper opening, 26 ... expanded upper opening end, 2
7 ... Guide tube, 28 ... Tip of gas ejection nozzle.
フロントページの続き (51)Int.Cl.6 識別記号 庁内整理番号 ＦＩ 技術表示箇所 Ｂ０１Ｄ 53/62 Ｃ０１Ｂ 31/20 ＺＡＢ Ｚ Ｃ１２Ｎ 1/00 Ｂ 8828−4Ｂ Continuation of the front page (51) Int.Cl. 6 Identification code Office reference number FI technical display location B01D 53/62 C01B 31/20 ZAB Z C12N 1/00 B 8828-4B
Claims (1)
器と、該中空容器の下部を形成する培養槽と、該培養槽
に設けられた培養液の温度制御手段と、前記培養槽の中
心部に設けられ、前記ドーム型の上壁面に向かって培養
液を噴出する気液混合エジェクターと、該気液混合エジ
ェクターに連結された炭素含有ガスの供給源と、前記ド
ーム型上壁面の上部または下部空間部に設けられた光源
を有することを特徴とする培養液膜形成方式による光合
成培養装置。1. A hollow container having a transparent dome-shaped upper wall surface, a culture tank forming a lower portion of the hollow container, a temperature control means for a culture solution provided in the culture tank, and a center of the culture tank. And a gas-liquid mixing ejector for ejecting the culture solution toward the dome-shaped upper wall surface, a carbon-containing gas supply source connected to the gas-liquid mixing ejector, and an upper part of the dome-shaped upper wall surface or A photosynthetic culture device using a culture solution film formation method, comprising a light source provided in a lower space.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP6183776A JPH0838159A (en) | 1994-08-04 | 1994-08-04 | Photosynthetic culturing apparatus by culture liquid film forming system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP6183776A JPH0838159A (en) | 1994-08-04 | 1994-08-04 | Photosynthetic culturing apparatus by culture liquid film forming system |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH0838159A true JPH0838159A (en) | 1996-02-13 |
Family
ID=16141754
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP6183776A Pending JPH0838159A (en) | 1994-08-04 | 1994-08-04 | Photosynthetic culturing apparatus by culture liquid film forming system |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0838159A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100432828B1 (en) * | 2001-03-19 | 2004-05-24 | 라파즈 한라 시멘트 주식회사 | Biological CO2 fixation method using semi-continuous and series operation |
| JP2012023978A (en) * | 2010-07-20 | 2012-02-09 | Hitachi Plant Technologies Ltd | Apparatus and method for culturing photosynthetic microorganism |
-
1994
- 1994-08-04 JP JP6183776A patent/JPH0838159A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100432828B1 (en) * | 2001-03-19 | 2004-05-24 | 라파즈 한라 시멘트 주식회사 | Biological CO2 fixation method using semi-continuous and series operation |
| JP2012023978A (en) * | 2010-07-20 | 2012-02-09 | Hitachi Plant Technologies Ltd | Apparatus and method for culturing photosynthetic microorganism |
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