JPH08332075A - Culture of microbe and culture system - Google Patents

Abstract

PURPOSE: To easily provide at low cost both efficient culture method and culture system for microbes. CONSTITUTION: An activator, facultative anaerobic bacteria and indigenous bacteria are put into a 1st reaction vessel 1 which is then fed with a stock liquor, and the stock liquor is anaerobically reacted under a small amount of aeration to effect primary activation. A second batch of the activator is put into a 2nd reaction vessel 2 which is then fed with the primarily activated stock liquor to make an anaerobic reaction of the stock liquor under a small amount of aeration to effect secondary activation. A third batch of the activator is put into a 3rd reaction vessel 3 which is then fed with the secondarily activated stock liquor to make an anaerobic reaction of the stock liquor under a small amount of aeration to effect tertiary activation.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a microorganism culturing method and a culturing apparatus.

[0002]

2. Description of the Related Art Conventionally, methods and apparatuses for culturing microorganisms have the drawbacks of low efficiency and high cost. The present invention intends to provide a method and a device for culturing a microorganism, which can treat a raw material much more easily, efficiently, and cheaper than ever by culturing a microorganism.

[0003]

SUMMARY OF THE INVENTION It is an object of the present invention to provide a method and an apparatus for culturing a microorganism, by which the raw material can be treated simply, efficiently and inexpensively by culturing the microorganism.

Another object of the present invention is to provide a method and an apparatus for culturing microorganisms, which can treat human waste in humans in a simple, efficient and inexpensive manner by culturing the microorganisms.

Another object of the present invention is to provide a method and an apparatus for culturing microorganisms, which purifies a pond or a river by culturing microorganisms.

In the present invention, an activator, a facultative anaerobic bacterium, and an indigenous bacterium are contained in a first reaction tank to receive a supply of a raw material liquid,
The raw material liquid is anaerobically reacted under a small amount of aeration for primary activation, the activator is contained in the second reaction tank, and the primary activated raw material liquid is supplied to perform primary activation under a small amount of aeration. The activated raw material liquid is secondarily activated by anaerobic reaction, the activator is accommodated in the third reaction tank, the secondary activated raw material liquid is supplied, and the raw material liquid is secondarily activated under a small amount of aeration. Is a method for culturing a microorganism, characterized in that the bacterium is subjected to an anaerobic reaction to carry out tertiary activation.

The present invention also accommodates a facultative anaerobic bacterium, an activator, and an indigenous bacterium to receive a supply of a raw material liquid, and anaerobically reacts the raw material liquid under a small amount of aeration to perform primary activation. And a second reaction tank that receives the supply of the raw material liquid that contains the activator and is primarily activated, and secondarily activates the raw material liquid by anaerobic reaction under a small amount of aeration. A third reaction tank that receives the supply of the raw material liquid that contains the agent and is secondarily activated, and anaerobically reacts the raw material liquid under a small amount of aeration to perform the third activation;
And a ventilating device for ventilating the third reaction tank.

The indigenous bacteria are bacteria that are indigenous to the land, and include, for example, soil, pond mud, sludge, sewage sludge, and / or humus soil. In some cases, lactic acid bacteria existing in the air of the land are also used. The indigenous bacteria are in the form of fermented by mixing ready-made compost and indigenous bacteria of the land, and the amount is 5 to 1 ton of the raw material liquid.
20 kg, usually 5-10 kg is used.

The raw material liquid comprises at least one or more of protein, cellulose, starch, molasses, carbohydrate, animal and vegetable fats and animal excrement, mineral liquid and water, but when it already contains a sufficient amount of water, The addition of new water is omitted.

The mineral liquid is trade name "MINEX G",
Those commercially available as “Microl A”, “Microl B”, “Microl C”, “Microl D” and “Microl for microorganisms” are suitable. 10 l is used per 1 t of the raw material liquid at the start-up, and 1 l is used per 1 t of the raw material liquid from the next replenishment.

Facultative anaerobic bacteria are, for example, Strepto-mycessp. (ATCC 3004), St.
reptoverticillium sp. (ATCC 23654), Nocardia sp.
(ATCC 19247), Micromonospora sp. (ATCC 12452), Rho
dococcus sp .: As belonging to photosynthetic bacteria, for example
Rhodopseudomonas sp. (R. sphaeroides), Rhodospiri
llum sp. (R. fulum), Chromatium sp. (C. okenii), C
hlorobium sp. (C. Limicola): Lactic acid bacteria (lactic acid-producing bacteria)
Belong to, for example, Lactobacillus sp. (IFO
3070), Propionibactrium sp. (P. freudonreichii),
Pediococcus sp. (P. halophilus), Streptococcus sp.
(S. lactis, S. faecalis); As a filamentous fungus, for example, Aspergillus sp. (RIFY 5770, RIFY 5024),
Mucor sp. (IFO 8567); As belonging to yeast, for example, Saccharomyces sp. (NRRL 1346, Y 977), Candida
There is sp. (C. utilis).

As the facultative anaerobic bacterium, one commercially available under the trade name of "Callus NC-R" is preferable. In addition, protein-digesting bacteria, starch-digesting bacteria, cellulose-digesting bacteria, activator-digesting bacteria and / or animal and vegetable oil and fat-digesting bacteria are preferable. As such, there are five kinds of mixed bacteria, which are commercially available from Nittek Co., Ltd. under the trade name of "grease cutting".
There are mixed-species bacteria. Table 1 shows the characteristics of Callus NC-R.

[0013]

[Table 1] "Grease cutting" or "Callus NC-R" is used in an amount of 1 to 5 kg per ton of raw material liquid.

As the activator, a molded product composed of carbon, diatomaceous earth, ferrite and zeolite or a molded product composed of diatomaceous earth is primarily calcined at 150 to 250 ° C. for 10 to 20 hours, and then secondarily at 600 to 900 ° C. for 4 to 24 hours. It was baked. The molded product can be a plate, a sphere, or any other shape, but is usually a sphere. In the case of spheres, it is composed of a single layer of spheres or of a core, one or more mesophases and a porous outer layer.

The core is made of diatomaceous earth, the intermediate layer is made of conductive carbon, ferrite and zeolite, and the porous outer layer is made of diatomaceous earth and zeolite.

The ferrite mixed in the intermediate layer is magnetite (Fe ₃ O ₄ ) and maghemite (γ-Fe ₂ O ₃ ) by natural deposition. This has the advantage of not eluting.

The activator is a super wavelength (10 ⁴ Hz) to X-ray (1
0 ¹⁹ Hz), especially electromagnetic waves of 60 to 200 MHz are absorbed (electromagnetic waves of wavelengths shorter than that are absorbed by increasing the number), and so-called coherent electromagnetic waves of uniform phase and good coherence are emitted. It emits far-infrared rays with a wavelength of 10 μm, has the function of eluting minerals and adsorbing impurities, and reduces the clusters of water molecules.

When a large number of active agents are collected in one place, transmission, resonance and amplification occur. The active agent has a diameter of about 10, 20, 30,
As the spheres of 40, 50 and 60 mm, those commercially available from the applicant under the trade name of "Minemaru-kun" are suitable. The activator is about 5 to 20 per 1 t of the raw material liquid in each reaction tank.
Kg, usually 7 to 15 kg is used.

[0019]

When the components of the raw material liquid are mixed and stirred in the raw material tank, the organic matter group in the raw material liquid is prevented from spoiling by an enzymatic reaction with minerals, and the microbial flora of the raw material liquid is controlled so that spoilage bacteria are not generated. If the enzymatic reaction with minerals does not proceed well, it will have a rotten odor.

The first reaction tank is loaded with indigenous bacteria of the area, facultative anaerobic bacteria are added, and the activator is suspended, and 20 to 3 is added.
Aerate for 0 days. In the raw material tank, organic substances and microbial groups in the raw material liquid come into contact with indigenous bacteria in the area, and the organic substances grow as food and are activated by minerals. The indigenous bacteria in the area are used to activate and multiply the microorganisms in the area.

In the raw material liquid from the raw material tank activated with minerals, a group of microorganisms that have been gradually strengthened by the mixing of new bacteria called facultative anaerobic bacteria remains. Since the active agent further maintains the mineral effect and absorbs and dissipates space energy as electromagnetic waves in order to stabilize water and microbial flora, a high energy mixed solution is completed.

Minerals, organic substances, facultative anaerobic bacteria and indigenous bacteria in the first reaction tank are well adapted in the reaction for 20 to 30 days, and are further adapted in the reaction in the second reaction tank for 20 to 30 days.

In the second reaction tank, minerals, indigenous bacteria, and organic acids produced together with the activator drastically reduce general bacteria and increase filamentous fungus groups.

When acclimated to the third reaction tank for another 15 to 30 days, a group of radiant bacteria and a group of yeasts (both of which are indigenous bacterial groups in the area) proliferate, and filamentous fungi almost disappear. First to first
The bacterial flora changes as the third reaction tank and the tank change.

In the third reaction tank, the bacterium group and water sufficiently receive the wave energy from the activator as an electromagnetic wave, and a remarkably activated solution (including the bacterium group) is completed. This solution consists of minerals, actinomycetes, yeast, and water, and also absorbs enough space energy.

The product liquid of the third reaction tank enters the storage tank, and this becomes the stock solution of the animal and plant activating liquid. Since this liquid has a high concentration, diarrhea in animals and disorders such as growth stop of crops appear if they are left as they are. Therefore, they are usually diluted appropriately with water before use.

In the diluting tank, the high-concentration solution in the storage tank is about 50
Dilute 0-1500 times. This not only dilutes, but stabilizes the solution by uniformly dispersing the concentrated solution in water.

In order to give more energy to the stabilizing solution, it is preferable to use 10 to 15 kg of activator per 1 ton of the solution even in the diluting tank. The stabilized product can be used for drinking water for animals and for growing whole crops.

If the activator is housed in a net container and suspended in each tank, it is convenient for treatment such as installation and replacement of the activator, and there is no clogging so that distribution and aeration are easy. , Activation is promoted.

The mesh container is preferably made of metal mesh. Stainless steel is preferable from the viewpoint of corrosion resistance. The activation action of the activator is determined not only by the amount of the activator contained but also by the height from the bottom surface to the top surface of the net container, and this height determines the electromagnetic wave absorption capacity. When the antenna is attached to the net container, the tip of the antenna works the same as the upper surface of the net container, so the height of the net container is the same as the height of the net container. Is promoted. The antenna preferably has a height at least as shown in FIGS. 1 and 2. 3 when the reaction liquids of the first to third reaction tanks are in communication
It is preferable to increase the height of the antennas of the book collectively.
A 1 m antenna produces a potential difference of 1 Volt. The potential difference varies depending on the weather, and it is rainy frequently.

If a raw material tank is provided in front of the first reaction tank and each component of the raw material liquid is received and mixed therein, it is possible to supply a homogeneous raw material liquid which is rot-proof to the first reaction tank.

The raw material liquid which has been tertiary activated becomes an active product liquid. Providing a storage tank for storing the product liquid after the third reaction tank is convenient for storing, handling, treating and selling the product liquid.

The product liquid is about 500 to 20 in use.
It is diluted to 00 times, usually about 1000 times, and used for vegetables, livestock, rice cultivation and so on. A diluting tank is provided for the purpose of diluting,
A highly active diluting liquid can be used by suspending a net-shaped container holding an activator in a tank, diluting the produced liquid, and then holding it for several days and then sequentially using it.

The pH of the raw material liquid is adjusted in the preliminary tank and the first to third reaction tanks.
Is changing. Normal pH is about 8.4 in preliminary tank, first
A reaction tank 8.4, a second reaction tank 8.8, and a third reaction tank 9.1. When the pH becomes 9 or more, Escherichia coli and Salmonella die.

Aeration is preferably about ^{3 to} 5 l / min per 1 m ³ of volume of each of the first, second and third reaction tanks. Below about 3 l / min, the effect of aeration is poor, and above about 5 l / min, the anaerobic reaction of facultative anaerobic bacteria is impaired.

When minerals are allowed to act on the indigenous bacteria, initially,
It has been found that the general bacteria and the filamentous fungus group are mainly contained, but the general bacteria and the filamentous fungus group are drastically reduced in 2 to 3 days, and the actinomycetes and yeast are remarkably increased. Further, if the malodor of processed fish and shellfish and the malodor of compost making are sprinkled with minerals, the malodor will decrease drastically with time, and the fermentation route of compost will change.

When the diluted production liquid was sprayed on the compost raw material for composting, a high quality compost which could not be considered in the past was produced.

When the produced liquid is sprayed in a septic tank for humans or livestock in an amount of 1 liter per 1 ton of the tank liquid, the odor disappears and 5
The odor disappeared when the powder diluted to 100 to 1500 times was sprayed.

By returning the produced liquid to the raw material tank, the activation of indigenous bacteria and the familiarization of facultative anaerobic bacteria can be accelerated. The amount to be returned is usually 20 to 40 kg per ton of the raw material liquid.

[0040]

EXAMPLES The present invention will now be described in more detail with reference to examples.

Example 1 In the apparatus shown in FIG. 1, a preparatory tank P having a capacity of 500 l contains cow urine, a mineral liquid marketed under the trade name of "Microl", and sewage from the garden 15%: 1% : A raw material liquid containing 84% by weight at a pH of about 8.4 and a volume of 100
It is constantly supplied to 0 l of the first reaction tank 1 at a rate of about 40 l / day. The raw material tank R was aerated and agitated by a pump at any time.

The first reaction tank contains a stainless steel container 5 containing 10 kg of the activator 4, 5 kg of facultative anaerobic bacteria in the form of "Callus NC-R", and indigenous bacteria from the applicant. Compost sold under the trade name "Green Top" is mixed with white indigenous bacteria collected from bamboo yam and nutrient source (rice bran) and fermented for 7 days. Aeration is performed at a rate of 3 liters per minute, and the raw material liquid is adjusted to about 8.4 at pH 8.4
It was treated for 0 to 30 days to be primary activated, and then supplied to the second reaction tank 2 having a capacity of 1000 l by vulcanization.

The second reaction vessel 2 receives the raw material liquid which has been activated by the primary activation, suspends a metal mesh container 5 charged with 10 kg of the activator 4, and ventilates from below the container 5 at a rate of 3 l / min. , PH
After 20 to 30 days of treatment at 8.8, the secondary activated raw material liquid was supplied to the third reaction tank 3 having a volume of 1000 l by overflow.

The third reaction tank receives the secondarily activated raw material liquid, accommodates a stainless mesh container 5 charged with 10 kg of the activator 4, and aerates from below the container 5 at a rate of 3 l / min per minute. After about 15 to 30 days of treatment at pH of about 9.1, the tertiary activated raw material liquid (production liquid) was discharged to the storage tank Q by overflow. As the activator, a mixture of "Minemaru-kun" having various particle sizes was used in the first to third reaction tanks.

The storage tank Q is for receiving and storing the produced liquid and has a capacity of 500 liters. The net container 5 has an antenna 6 having a height of 1.5 m.

Since the product solution thus produced is too thick as it is, it is usually diluted with water about 1000 times,
It is applied to vegetables and rice crops to obtain excellent effects such as yield improvement, quality improvement, and continuous crop failure prevention, and it is used for drinking water for livestock production to prevent stench from stalls, reduce disease incidence, and reduce mortality. Good results such as increased milk yield were obtained.

Example 2 The apparatus shown in FIG. 2 is almost the same as the apparatus shown in FIG.
A product consisting of 1% by weight of mineral liquid marketed under the trade name "MICROL" and 99% by weight of the supernatant of the combined septic tank is used as the raw material liquid, and the product of "Grease cutting" from Nitek Co., Ltd. in the first reaction tank. Under the name, a container 7 containing 5 kg of commercially available facultative anaerobic bacteria and a container 8 containing indigenous bacteria were suspended, and a container 5 containing 10 kg of the activator 4 was also suspended in the water tank 6. As a result of the treatment, the purification capacity is 50 compared with the control device.
% Improved.

Example 3 Molasses was used instead of cow urine as a raw material liquid, and the same operation as in Example 1 was carried out to obtain almost the same results as in Example 1. When molasses was used as the raw material liquid, it was preferable to make it from brown sugar. Since white sugar has no mineral content, it was necessary to add mineral content.

Example 4 The pig farm in Niigata did not have sufficient treatment capacity for 4 m ³ / day of urine from pigs, but by operating in the same manner as in Example 2, a conventional septic tank of 2.5 m ³ / day is sufficient. As a result, the pH adjusting agent is no longer needed.

[0050]

The effects are shown below. (i) Overcoming continuous cropping obstacles. (ii) Improvement of crop quality and increase of yield. (iii) Cost reduction by reducing fertilizer and pesticide costs. (iv) Removal of stench and flies in the barn. (V) Reduced morbidity and mortality. (Vi) Significant reduction in drug usage such as antibiotics. (Vii) Ensuring the safety of meat, eggs and milk. (Viii) Improvement of feed efficiency. (iX) Improving milk quantity and milk quality stability. (X) Significantly reduced mastitis. (Xi) Realization of high quality feed crops and silage production by utilizing compost and produced liquid produced from human waste. (Xii) The stress of the cow is released quickly. The litter is greatly reduced by returning the compost to the livestock shed. (Xiii) Improvement of meat quality and increase of body weight gain per day. (XiV) Reduced egg breaking rate. Improvement of egg quality. Stable antibodyization of vaccine. Alleviation of noise. (XV) Improvement of gain of broiler. Improvement of meat quality. Decrease in butt pecking. (XVi) Applying to drinking water makes delicious water easy. (XVii) 40-50% improvement in septic tank capacity.

[Brief description of drawings]

FIG. 1 is an explanatory diagram showing an example of a device of the present invention.

FIG. 2 is an explanatory diagram showing another example of the device of the present invention.

[Explanation of symbols]

 P Raw material tank 1 1st reaction tank 2 2nd reaction tank 3 3rd reaction tank 4 Activator 5 Metal net container 6 Antenna 7 Container containing facultative anaerobic bacteria 8 Container containing indigenous bacteria Q Storage tank R Dilution tank

Claims (21)

[Claims] 1. An activator, a facultative anaerobic bacterium, and an indigenous bacterium are contained in a first reaction tank to receive a supply of a raw material liquid, and the raw material liquid is anaerobically reacted under a small amount of aeration. Primary activation, the activator is housed in the second reaction tank, the primary activated raw material liquid is supplied, and the primary activated raw material liquid is secondarily activated by anaerobic reaction under a small amount of aeration. It is characterized in that the activator is contained in the third reaction tank, the secondary activated raw material liquid is supplied, and the secondary activated raw material liquid is anaerobically reacted under a small amount of aeration for tertiary activation. Method for culturing microorganisms to be used. 2. The method according to claim 1, wherein the raw material liquid contains at least one kind of protein, cellulose, molasses, starch, carbohydrate, animal and vegetable fats and animal excrement, and a mineral liquid. 3. The method according to claim 1, wherein the facultative anaerobic bacterium is callus NC-R. 4. The method according to claim 1, wherein a molded article in which the activator is made of diatomaceous earth or a molded article made of carbon, diatomaceous earth, ferrite and zeolite is primarily calcined at 150 to 250 ° C. for 10 to 20 hours, and then 600 to A method in which secondary firing is performed at 900 ° C. for 4 to 24 hours. 5. The method of claim 4, wherein the active agent is a sphere consisting of a single layer of spheres or nuclei, one or more intermediate layers and a porous outer layer. 6. The method according to claim 5, wherein the activator core comprises diatomaceous earth, the intermediate layer comprises conductive carbon, ferrite and zeolite, and the porous outer layer comprises diatomaceous earth and zeolite. 7. The method according to claim 1, wherein a raw material tank is provided before the first reaction tank, and the components of the raw material liquid are mixed therein to form a raw material liquid. 8. The method according to claim 1, wherein the mineral liquid is a commercially available mineral liquid. 9. The method according to claim 1, wherein the product liquid is stored after the third reaction tank. 10. The method according to claim 9, wherein the activation liquid is diluted after the storage tank and is further activated by an activator. 11. A first activation for accommodating a facultative anaerobic bacterium, an activator, and an indigenous bacterium, receiving a supply of a raw material liquid, and causing an anaerobic reaction of the raw material liquid under a small amount of aeration to perform primary activation. Receiving the supply of the reaction tank and the raw material liquid that contains the activator and is primarily activated,
A second reaction tank that secondarily activates the raw material liquid by anaerobic reaction under a small amount of aeration, and is supplied with the raw material liquid that contains the activator and is secondarily activated.
Microorganisms characterized by comprising: a third reaction tank for anaerobically reacting a raw material liquid under a small amount of aeration for tertiary activation; and an aeration device for aeration of the first, second and third reaction tanks. Incubator. 12. The device according to claim 11, wherein facultative anaerobic bacteria are commercially available as Callus NC-R (trade name) or Grease Cutting (trade name). 13. The apparatus according to claim 11, wherein the activator is a molded product made of carbon, diatomaceous earth, ferrite and zeolite or a molded product made of diatomaceous earth is 150 to 250.
Primary calcination for 10 to 20 hours at 600C, then 600 to 900
An apparatus which is secondarily baked at 4 ° C. for 4 to 24 hours. 14. The apparatus according to claim 13, wherein the molded product made of carbon, diatomaceous earth, ferrite and zeolite has a structure having a core, one or more intermediate layers and a porous outer layer, and the core is made of diatomaceous earth, A device in which the middle layer is made of conductive carbon, ferrite and zeolite and the porous outer layer is a sphere made of diatomaceous earth and zeolite. 15. The device according to claim 13, wherein the molding is a sphere. 16. The device according to claim 11, wherein the activator is contained in a net container and suspended in each tank. 17. The apparatus according to claim 16, wherein the net container is made of metal. 18. The device according to claim 17, wherein the antenna is attached to a net container. 19. The apparatus according to claim 11, wherein the third
An apparatus having a storage tank for storing the produced liquid after the reaction tank. 20. The device according to claim 19, further comprising a diluting tank for diluting the product liquid after the storage tank and further activating the resulting liquid with an activator. 21. A product solution obtained by the culture method of claim 1.