JPH0632708A - Microbial material and its production - Google Patents

Abstract

PURPOSE:To obtain a microbial material composed of a fermentation treatment product of a mixture of soybean broth with a clay mineral and an additive. CONSTITUTION:This microbial material is obtained by adding and mixing a clay mineral (e.g. zeolite, perlite, ferrite or montmorillonite), usually and widely used as artificial soil or a soil improving agent and an additive capable of optimizing growth conditions of microorganisms (e.g. a calcium mixture) with a soybean broth discharged from a heating step or a steaming or boiling step of a processing factory for soybeans, inoculating a spawn such as Bacillus subtilis, actinomyes or yeast, preferably a microorganism decomposing or utilizing malodorous substances or a microorganism having antagonistic action on soil rot fungi into the resultant mixture and forcedly aerating or heating the obtained product after completing the fermentation. This material comprises live cells or spores at a high concentration and can effectively be utilized for preventing malodor of sewage sludges, raw sewage treating sludges, excreta treating sludges, agricultural, forestry and fisheries wastes, surplus activated sludges, household refuses, etc., and also as a soil improving agent for green and agricultural land, a preventing agent for injury by continuous cropping, a soil pathogenic germ suppressor, etc.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a microbial material using soybean juice and clay mineral discharged from a soybean processing factory as main raw materials and a method for producing the same.

[0002]

BACKGROUND OF THE INVENTION There are many legume processing plants in Japan,
Above all, in soybean processing plants and natto manufacturing plants, a large amount of soybean broth containing high-concentration organic matter is produced as a by-product from the soybean processing process. At present, this soybean juice is either diluted and treated with wastewater by the activated sludge method or concentrated and incinerated until it is not effectively used.

[0003]

However, the conventional processing method as described above has a problem that a large amount of expenses are required in terms of equipment investment and running cost. Therefore, as an attempt to effectively utilize this soybean juice, an example of wastewater treatment by culturing yeast and recovery of bacterial protein,
Alternatively, studies have been conducted so far to carry out lactic acid fermentation to make it into a food material, but the current situation is that it has not been put into practical use,
A new usage was eagerly awaited.

[0004]

[Means for Solving the Problems] The present invention has been made to solve the above-mentioned conventional problems and contributes to the prevention of pollution by effectively utilizing this soybean juice that has not been used conventionally. It is intended to provide useful things such as soil improvement of agricultural land and improvement of bad odor environment, and the summary is from fermentation treatment of mixture of soybean juice and clay minerals and additives. Microbial material characterized by becoming, and by adding and mixing clay minerals and additives to soybean juice,
Next, the method for producing a microbial material is characterized in that the mixture is fermented.

To explain the present invention in detail, the soybean juice used in the present invention is discharged from a heating process or a steaming process in a soybean processing plant, and has an extract content of 3
It is an organic waste water that contains water-soluble organic matter in soybean in a high concentration. Above all, the soybean juice discharged from the miso factory and the natto factory is large in amount and easy to collect, so it is suitable for practical use.

[0006] As the organic matter of soybean juice, there are many polysaccharides such as arabinogalactan, galactomannan, and high molecular weight galacturonic acid. The main component is glycoprotein and water-soluble soybean protein to which proteins are bound, and other inorganic matter. , Since it contains a relatively large amount of phosphoric acid, potassium, sodium, etc.
It is suitable for the growth of microorganisms. Taking the soybean juice discharged from the miso factory as an example, the extract content is 3 to 6%, the total sugar is 1 to 3%, the direct reducing sugar is 0.5 to 1.5%, and the total nitrogen is 0.
In the method of the present invention, the soybean juice can be used as it is at a concentration of 05 to 0.15% and a pH of 5 to 6, or the extract can be concentrated to 30 to 40% in advance and used.

Further, as the clay mineral used in the present invention,
Generally, substances widely used as artificial soils or soil conditioners, for example, zeolite, perlite, vermiculite, ferrite, soft silica, montmorillonite, clay ball and the like are mentioned, and these may be used alone or in combination of two or more. It doesn't matter. As the particle size of this clay mineral, it is preferable that the amount of porosity (volume of the entire clay minus the volume of the solid phase) is larger, and 0.5 to 2 mm is more preferable than fine powder, It is not limited.

Furthermore, the additive used in the present invention is for optimizing the growth conditions of microorganisms, and examples thereof include calcium compounds such as shellfish powder, calcium carbonate, and slaked lime. Eg 5
~ 30% added and used to maintain PH during fermentation in the range of 6-9. If necessary, a small amount of glucose, sucrose, a sugar such as starch, a nitrogen source such as ammonium sulfate or urea, an inorganic salt such as potassium phosphate or magnesium sulfate may be supplemented as a growth promoting agent for the microorganism.

[0009] Thus, by adding and mixing the clay minerals and additives to the soybean juice, the growth action of microorganisms proceeds smoothly and a high-concentration microbial material can be obtained. That is, the clay mineral and the additives are mixed in approximately equal amounts by weight in the soybean juice to adjust the water content suitable for fermentation of the microorganism. This water content is 30 to 70 at the start of fermentation.
%, Preferably 40 to 50%, to improve air permeability and inoculate seeds.

[0010] Examples of the microorganisms used as the inoculum include Bacillus subtilis, actinomycetes, yeasts, etc. These microorganisms may be commercially available commercially available microbial materials for agriculture, or animal dung. , It may be used as it is easily separated from compost, sludge, etc. Of these, microorganisms that decompose or utilize malodorous substances such as ammonia, trimethylamine, mercaptan, and hydrogen sulfide, and microorganisms that have an antagonistic action against soil-rotting bacteria are preferable, and these may be used alone or in combination of two or more. These microorganisms may be pre-cultured, or a part of them may be preserved after the main culture and reused as an inoculum.

After inoculation of the inoculum, fermentation is carried out by forced ventilation in a culture tank having a ventilation tube at the bottom, but the fermentation apparatus and shape are not particularly limited. 30 ° to 4 when the temperature is low
Fermentation is promoted by blowing in 0 ° C warm air, and in the end, the heat of fermentation is generated by the activity of microorganisms and the temperature rises.
When it reaches 0 ° to 60 ° C, it is cut back, and further fermentation is sufficiently achieved. The fermentation time is 2-5 days.

The fermented product thus obtained can be easily dried by evaporating the internal water content by forced aeration or heating to form a microbial material containing a high concentration of viable cells and spores. You can The bacterial count of such materials reaches 10 ⁸ to 10 ¹⁰ per gram. The microbial material thus obtained can be effectively used for preventing the bad odor of sewage sludge, human waste sludge, excrement, agricultural, forestry and fishery waste, excess activated sludge, household waste, etc. It can also be used as a preventive agent for continuous cropping disorder and as a deterrent agent for soil pathogens.

Hereinafter, the present invention will be described in more detail with reference to Examples. [Example 1] As an inoculum, a plant disease fungus (Fusari)
Bacillus subt., which has an inhibitory effect on um oxysporum)
ilis) was added to this Bacillus subtilis in advance with 2% glucose, 1% polypeptone, 0.1% potassium dibasic phosphate and 0.05% magnesium sulfate to obtain a pH of 6.8, and the medium was sterilized. At 37 ° C. for 48 hours, aeration culture was performed to obtain a seed culture solution. As a result of measuring the number of bacteria in this culture solution,
It was 1.8 × 10 ⁹ / ml.

Approximately 100 l of soybean juice was sampled from the miso factory as a fermentation raw material, and part of its components was analyzed. Total organic carbon content was 11,620 mg / l and total nitrogen content was 648.
The results were: mg / l, total phosphoric acid 74 mg / l, COD (chemical oxygen demand) 24,000 mg / l, BOD (biological oxygen demand) 31,000 mg / l, PH 5.2. 100 kg of zeolite containing 80% or more of the soybean juice having a particle size of 0.5 to 1.68 mm and oyster powder 20 k
It was added to a mixture with g and inoculated with 4 liters of the inoculum solution described above, followed by stirring and homogenizing with a mixer. The water content at this time was about 42%.

Next, the above mixture is put into a fermenter (open on top, open on one side, has a bottom vent pipe), and the deposition height is 65c.
About 5% (v / v) of warm air (35 ° C to 45 ° m / min)
Fermentation was performed by blowing C). 24-48 from the start of fermentation
Over time, the internal temperature rose to 50-60 ° C, where the sediment was cut back and the fermentation continued and the temperature dropped from 72 hours and the PH began to rise. From this time, the amount of aeration was increased to accelerate the drying, and at the same time, the spore formation of the fungus was promoted and stable microbial material was obtained. The yield is about 124
In kg, the number of bacteria in 1 gram was 2.6 × 10 ⁹ .

The microbial material obtained here was treated with hydrogen sulfide 4
200mg / in sewage concentrated sludge with a concentration of 2% that produces offensive odors of about 00ppm and methyl mercaptan 50ppm
After adding 1 liter and aerating for 4 hours, the generation amount of malodorous substances was measured with a gas detector tube. In the control group without addition, the generation amounts of hydrogen sulfide and methyl mercaptan did not decrease,
In the area where the microbial material was added, it was not detected and a remarkable deodorizing effect was observed.

[Example 2] A radiant bacterium (BFB manufactured by Midori Sangyo Co., Ltd.) having an inhibitory effect on pathogens such as Fusarium, Pythium, and Rhizoctonia in soil was used as an inoculum, and 2 kg thereof was used in Example 1 above. Suspended in 100 liters of the same composition liquid as the soybean juice used in 1., and 80 kg of perlite (rough porosity 30 to 33%) and oyster powder 2
After adding to 0 kg of the mixture and thoroughly stirring and homogenizing with a mixer, fermentation was carried out in the same manner as in Example 1 below.
After 5 days, about 106 kg of microbial material was obtained. The number of bacteria was 1.2 × 10 ⁹ per gram.

Example 3 As a microorganism, yeast (Torulo
psis xylinus OUT 6182), using 3% waste sugar dense, 0.1% potassium dibasic phosphate, 0.05% magnesium sulfate,
Aeration culture was carried out at 30 ° C. for 48 hours in a pH 7.0 sterilized medium to obtain a seed culture. 2 l of this inoculum solution was inoculated into a mixture of 50 l of soybean juice having the same tissue as in Example 1, 50 kg of granular zeolite, and 10 kg of soft silica, and fermentation was carried out in the same manner as in Example 2 below. Obtained. The number of bacteria was 9.6 × 10 ⁸ per gram.

[0019]

EFFECTS OF THE INVENTION The microbial material according to the present invention has the following effects because it is composed of a fermented product of a mixture of soybean juice, clay minerals and additives as described above. That is, (1) the unused soybean juice can be obtained by mixing the soybean juice in its original form without treatment such as concentration and dilution. (2) Due to the characteristics of the clay mineral used in the present invention, solid static culturing can be smoothly carried out, and therefore large capital investment such as tank culturing carried out in the fermentation industry is not required. (3) The fermented product can be easily air-dried due to its physical characteristics, and the drying cost is low. (4) The microbial material obtained by the present invention is
Since the microorganisms are firmly attached to the clay mineral, it is stable for a long period of time during storage, and even when applied to soil or sludge, it fully exhibits its bioactive function. In particular,
When applied to soil, in addition to bioactivity, improvement of soil physical properties, improvement of soil chemistry, and supply of nutrients to plants become remarkable. (5) The soybean broth, which will increase in number and is difficult to dispose of in the future, can be used effectively as a deodorizing material for environmental pollution, because it can effectively use resources.
It is also effective in terms of pollution prevention and has a wide range of uses.

Claims (2)

[Claims] 1. A microbial material comprising a fermented product of a mixture of soybean juice, clay minerals and additives. 2. A method for producing a microbial material, which comprises adding and mixing clay minerals and additives to soybean juice, and then fermenting the mixture.