JPH083018A - Cosmetic for skin aging prevention - Google Patents

Abstract

PURPOSE:To provide a cosmetic which contains superoxide dismutase, an antioxidant enzyme, develops its enzymatic activity effectively because the activity is stabilized, thus prevents effectively skin aging with no trouble in safety. CONSTITUTION:This cosmetic for skin aging prevention is prepared by adding 0.005-0.05 pt.wt. of superoxide dismutase extracted from bacteria, calculated as protein per 100 pts.wt. of a cream base containing 1-10wt.% of hexadecyl alcohol, 20-45wt.% of silicone oil, 15-40wt.% of glycerol, 0.3-7.0wt.% of a phosphoric salt and 5-25wt.% of carboxymethyl cellulose as essential ingredients.

Description

Detailed Description of the Invention

[0001]

FIELD OF THE INVENTION The present invention relates to a novel cosmetic for preventing skin aging, and more specifically, it contains a superoxide dismutase which is an antioxidant enzyme, and its enzyme activity is stably and effectively expressed to improve skin aging. The present invention relates to a cosmetic composition which can effectively prevent the above-mentioned and highly safe.

[0002]

2. Description of the Related Art Superoxide dismutase (Su
peroxide dismutase (hereinafter abbreviated as SOD) is also called a superoxide disproportionation enzyme and is an enzyme that all animals, from microorganisms to humans, have in their bodies.
An enzyme containing copper and zinc in the active center (Cu / Zn-SO
D), an enzyme containing manganese (Mn-SOD), and an enzyme containing iron (Fe-SOD) are known. Cu /
Zn-SOD is mainly distributed in eukaryotes, and Mn-SOD
D and Fe-SOD are mainly distributed in bacteria, actinomycetes, and fungi.

This SOD is an enzyme that catalyzes a disproportionation reaction of superoxide anion radical (O ₂ · ⁻ ) 2O ₂ · ⁻ + 2H ⁺ → O ₂ + H ₂ O ₂ , that is, active oxygen in the living body is converted into hydrogen peroxide. It is an enzyme that converts into oxygen. The active oxygen is considered to be one of the causes of tissue damage such as inflammation and aging. Therefore, SOD is considered to protect the organism from the toxicity of this active oxygen.

Since SOD has such physiological activity, it has recently been confirmed that it is effective as an anti-inflammatory agent for arthritis and rheumatism, and for Behcet's disease, ischemic heart disease, collagen disease and the like. However, its effectiveness is expected. Furthermore, in the dermatological field, skin ulcers,
It has been shown to be effective against keloid formation, sunburn, etc. Therefore, clinically applied research on SOD is being actively conducted.

On the other hand, in the field of cosmetics, research and development of cosmetics for preventing skin aging have been actively conducted, and various products have been put on the market. It is known from recent dermatological research that aging of the skin is caused by a decrease in moisturizing function with aging, accumulation of external stimuli such as ultraviolet rays, and a decrease in function of skin cells. Therefore, as a cosmetic for preventing skin aging, for example, an ultraviolet absorber, an ultraviolet reflection / scattering agent, arbutin or kojic acid having an action of suppressing the formation of melanin pigment by ultraviolet rays, hyaluronic acid or collagen having a moisturizing action are mixed. What has been done is being developed.

Furthermore, recently, active oxygen has been found to play an important role as one of the causes of skin aging. That is, squalene in the sebum membrane is oxidized by active oxygen to become an oxidizable lipid, and if this is left to stand,
It has been confirmed that lipids in the cell membranes of the epidermis and dermis are also oxidized to cause aging.

In addition, the skin surface has various biological, chemical,
It has also been found that when subjected to physical stress, for example, when irradiated with ultraviolet rays, a larger amount of active oxygen than usual is generated on the skin surface, or the function of removing active oxygen is reduced. Therefore, SO that decomposes active oxygen
It can be expected that D is effective as a component of a cosmetic composition for preventing skin aging.

When SOD is used as a component of cosmetics,
It is important that SOD has affinity or penetrability to the skin, its activity is effectively expressed, and it is stable, and that SOD can be easily produced and can be inexpensively and stably supplied.

[0009] Ordinary SOD has a short half-life in vivo and in vivo, and has a problem in affinity to tissue and permeability. Therefore, as a countermeasure against it, liposome formation using lecithin and binding with other substances are performed. Methods such as chemical modification such as or inclusion with an inclusive substance have been taken. Also, SOD
In the past, the production of SOD had been previously extracted and purified from bovine blood cells, but due to the limited raw materials, the production of human SOD from Escherichia coli by genetic recombination has been attempted in recent years.

However, the method for stabilizing SOD and improving the affinity and penetrability by the chemical modification or encapsulation as described above is complicated in operation and costly, and human SOD by gene recombination is extremely expensive. Since it requires complicated operations, it becomes expensive and is not practical as a component of cosmetics.

[0011]

Under the circumstances described above, the present invention contains SOD which is inexpensive and can be stably supplied,
The enzyme activity of which is stable and effectively expressed, can effectively prevent skin aging, and is intended to provide a highly safe cosmetic composition for preventing skin aging. .

[0012]

Means for Solving the Problems The present inventor has conducted extensive studies on SOD-containing cosmetics for preventing skin aging, and as a result,
SOD derived from bacteria can be mass-produced by culturing bacteria and can be stably supplied, and this SOD is mixed with a cream base of a specific composition in a predetermined ratio,
It was found that the enzyme activity is stable, the affinity and permeability of SOD to the skin are improved, the activity is effectively expressed, skin aging can be effectively prevented, and the safety is high. The present invention has been completed based on this finding.

That is, the present invention comprises 1 to 10% by weight of hexadecyl alcohol, 20 to 45% by weight of silicone oil,
Glycerin 15-40% by weight, phosphate 0.3-7.0
Wt% and carboxymethylcellulose 5 to 25 wt%
For preventing skin aging, 100 parts by weight of a cream base containing as an essential ingredient, superoxide dismutase extracted from bacteria in a proportion of 0.005 to 0.05 parts by weight as a protein amount. It is to provide cosmetics.

The SOD used in the cosmetic for preventing skin aging of the present invention is Mn-SOD or Fe-SOD obtained by extracting and purifying from cultured bacterial cells of bacteria. Examples of this bacterium include Pseudomona (Pseudomona)
s) genus, Bacillus genus, Micrococcus genus, Aeromonas (A)
genus Eromonas, Acetobacter
genus Lactobacillus flavobacter
erium), Gluconob (Gluconob)
Lactobacillus (Lactobacil)
illus), Rhizobium
Examples thereof include gram-positive bacteria and gram-negative bacteria such as genera and Legionella.

Specific examples of these bacteria include Pseudomonas syringae (IFO) as Pseudomonas bacteria.
3505), Pseudomonas radiola (Pseu)
domonas radiora, IAM 1209
8), Pseudomonas aeruginosa
onas aeruginosa, IAM 1237)
As a bacterium of the genus Bacillus, for example, Bacillus amyloliquefaciens
efaciens, IAM 1523), Bacillus stearothermophilus
Other bacterium of the genus Micrococcus, such as othermophilus, IAM 11002, is Micrococcus
Candidas (Micrococcus candidu)
s, IAM 12004), Micrococcus conglomerate
ratus, IAM 1459) and the like, Aeromonas liquefaciens (Ae
romonas liquidfaciens, IAM
12335), Aeromonas sigeroides (Aero)
monas shigelloides, ATCC 1
4029) and other bacteria belonging to the genus Acetobacter include Acetobacter acetogenus (Acetobacter).
acetigenus, IFO 3277), Acetobacter aurtus (Acetobacter au)
flavobacteria aquatile (F.
labobacterium aquatile, IA
M 12316) and the like include Gluconobacter zubuoxidans (Gluco).
nobacter subbox, IAM 1
2306), Lactobacillus brevis (Lactobacillus).
brevis, IAM1082), Lactobacillus casei, IA
M 1045) and the like, Rhizobium meliotte (Rhizobium mel)
iloti, IAM 12035), Rhizobium trifolli, A
HU 1134) and the like include Legionella pneumophila (Legionella p.
neumophila, ATCC 33154), Legionella pn.
eumphila, ATCC 43283) and the like. These bacteria can be easily obtained from domestic and foreign type culture collections.

The method for culturing these bacteria is not particularly limited, and a commonly used method can be used. However, a liquid culture method under a high oxygen partial pressure, for example, tryptocase or brine heart ink as a liquid medium is used. John,
It is preferable to use a nutrient or yeast extract liquid medium and the like, and to sterilize the bacterium under a high oxygen partial pressure by blowing sterile oxygen into the medium at a pH of about 7 and a temperature of about 30 to 37 ° C.

Next, SOD is extracted and purified from the cells thus cultured. The extraction / purification method is not particularly limited, and various methods conventionally used for extracting / purifying intracellular enzymes of bacteria can be used.

For example, the cultured cells are collected by centrifugation and frozen cells are obtained by a usual method using dry ice-acetone, and after thawing in an appropriate buffer,
The cells are crushed with a homogenizer and then centrifuged to obtain a crude enzyme solution (supernatant). Next, after adding ammonium sulfate or the like to this crude enzyme solution to perform salting out,
The precipitate is dialyzed with an appropriate buffer solution, the dialysate is appropriately concentrated, and then the SOD active fraction is obtained by column chromatography or the like. The SOD active fraction is dialyzed again with an appropriate buffer solution, concentrated, and then frozen to obtain a purified SOD. The protein amount of this purified SOD is, for example, 2 according to the Rory method.
It can be determined from the absorbance at 80 nm. The SOD thus obtained is of bacterial origin,
Mn-SOD or Fe containing manganese or iron in the active center
-SOD.

In the cosmetic for preventing skin aging of the present invention, 0.005 to 0.05 part by weight, preferably 0.01 to 0.03 part by weight, of the SOD is added to 100 parts by weight of the cream base. It is necessary to mix them in proportions of parts. If this amount is less than 0.005 parts by weight, the effect of preventing skin aging will not be fully exerted, and if it exceeds 0.05 parts by weight, the effect will not be improved much relative to the amount added, rather economical. Will be at a disadvantage.

The cream base used in the cosmetic composition for preventing skin aging of the present invention comprises hexadecyl alcohol 1 to 10% by weight, preferably 2 to 8% by weight, silicone oil 20 to 4
5% by weight, preferably 25-40% by weight, glycerin 1
5-40 wt%, preferably 20-35 wt%, phosphate 0.3-7.0 wt%, preferably 0.7-5.0 wt% and carboxymethylcellulose 5-25 wt%,
Preferably, it contains 10 to 20% by weight as an essential component. If the content of each compounding ingredient in this cream base deviates from the above range, problems will occur in the stability of SOD and the affinity and penetrability to the skin, and the effects of the present invention will not be sufficiently exhibited.

In the skin anti-aging cosmetic composition of the present invention, if desired, other components having a skin anti-aging effect, such as a UV absorber, a UV reflection / scattering agent, a melanin pigment production inhibitor, a moisturizing agent, etc. are blended. be able to.

Examples of ultraviolet absorbers include benzophenone compounds, benzotriazole compounds, cinnamic acid derivatives, and the like, and ultraviolet reflecting / scattering agents include
Examples thereof include titanium oxide, zinc oxide, kaolin, calcium carbonate, talc and the like.

The melanin production inhibitor includes, for example, arbutin, kojic acid and the like, and may further contain cytopron which has an effect of suppressing the conversion of melanin pigment into spots and freckles.

On the other hand, examples of moisturizing agents include amino acids,
NMF (natural moisturizing factor) such as pyrrolidone carboxylic acid, lactic acid, urea, and further hyaluronic acid, chondroitin sulfate, collagen, phospholipid, sphingolipid, chitin
Examples include chitosan.

Further, in the cosmetic for preventing skin aging of the present invention, in addition to the above-mentioned components, known ingredients which are usually used in creamy skin cosmetics, for example, an oil component, are used within a range not impairing the object of the present invention. , An emulsifier, a polyhydric alcohol, an antiseptic / antibacterial agent, a thickener, an antioxidant, a fragrance, vitamins, amino acids, water and the like can be added.

Examples of the oil component include liquid oils and fats such as castor oil, olive oil, jojoba oil and camellia oil, solid oils and fats such as hydrogenated castor oil, waxes such as lanolin, whale wax, beeswax, carnauba wax, candelilla wax and squalane. ,
Examples include hydrocarbons such as petrolatum, liquid paraffin, sericin, and paraffin.

Examples of the emulsifier include glycerin fatty acid ester, sorbitan fatty acid ester, propylene glycol fatty acid ester and soybean phospholipid, and examples of polyhydric alcohol include polyglycerin, trimethylolpropane, pentaerythritol, dipentaerythritol and ethylene. Glycol, propylene glycol, polypropylene glycol, 1,3-butylene glycol, 1,4-butylene glycol, further glucose, maltose, mannose, lactose, D-glucuronic acid, uronic acid, saccharose, D-mannitol,
Examples thereof include monosaccharides such as D-sorbit, sorbitan, gluconolactone, cellulose, starch and glucose phosphate, polysaccharides and derivatives thereof.

Examples of the antiseptic / antibacterial agent include methyl p-hydroxybenzoate, ethyl p-hydroxybenzoate, dehydroacetic acid, salicylic acid, benzoic acid, sorbic acid, benzalkonium chloride, and the thickeners. , For example, sodium alginate, xanthan gum, aluminum silicate, malomelo seed extract, tragacanth gum, natural macromolecules such as starch, methylcellulose, hydroxyethylcellulose, soluble starch, semi-synthetic macromolecules such as cationized cellulose, carboxyvinyl polymer, polyvinyl Examples thereof include synthetic polymer substances such as alcohol.

Examples of antioxidants include dibutylhydroxytoluene, butylhydroxyanisole,
Examples thereof include propyl gallate, ascorbic acid, and the like. Examples of the fragrance include plant-based natural fragrances such as fragrance, animal-based natural fragrances, and synthetic fragrances.

Examples of vitamins include vitamin A,
Vitamin B, vitamin C, vitamin D, vitamin E, vitamin F, vitamin K, vitamin P, vitamin U, carnitine, ferulic acid, α-oryzanol, α-riboic acid,
Orotic acid and its derivatives and the like, as the amino acids, for example, glycine, alanine, valine, leucine, isoleucine, serine, threonine, phenylalanine,
Examples thereof include tyrosine, tryptophan, cystine, cysteine, methionine, proline, hydroxyproline, aspartic acid, glutamic acid, arginine, histidine, lysine and derivatives thereof.

[0031]

EFFECT OF THE INVENTION The cosmetic for preventing skin aging according to the present invention comprises a cream base having a specific composition and a SOD derived from bacteria. The SOD decomposes the active oxygen, and Aging of the skin can be prevented. Further, in this cosmetic composition, the activity of the compounded SOD is stable, and the affinity and penetrability of SOD to the skin are good, so that the activity is effectively expressed and there is no problem in safety. There is no.

Furthermore, since the SOD to be blended is derived from bacteria, it can be mass-produced and an inexpensive one can be stably supplied.

[0033]

Next, the present invention will be described in more detail with reference to examples.

Reference Example 1 Bacteria shown in Table 1 were treated with tryptocase, brine heart fusion, nutrient or yeast extract liquid medium (all manufactured by Sigma) at pH 7.
The cells were cultured for 4 to 18 hours under the conditions of 0 and temperature of 30 to 37 ° C.
In addition, this culture uses an oxygen supply device and a dissolved oxygen measuring device (Tokyo Rika Co., Ltd. make, M-100 type), and 0.20-
6.50 M × 10 ⁶ aseptic oxygen was constantly blown into the medium, and the test was performed under high oxygen partial pressure.

Next, the cultured cells were collected by centrifugation at 4,000 × g and frozen with dry ice-acetone, and then 10 g of the frozen cells were
After thawing in 50 ml of 40 mM potassium phosphate buffer (pH 7.5) containing 0.1 M EDTA, and crushing the cells for 10 minutes at 4 ° C. and 5 KC with a Blanc homogenizer, 12, Centrifugation was performed at 000 G to obtain a supernatant as a crude enzyme. 70% by weight of (NH ₄ ) ₂ SO ₄ was added to the crude enzyme for salting out, followed by centrifugation at 14,000, and the resulting precipitate was added with 0.1 M potassium phosphate buffer solution. It was dissolved at (pH 7.8), adjusted to pH 7.8 again with a 1 M NaOH aqueous solution, and then dialyzed against 0.1 M potassium phosphate buffer (pH 7.8) overnight at 4 ° C.

This dialyzed solution was treated with an Amicon concentrator for about 7 minutes.
After concentrating to 0 times, DEAE-Sephadex A
Column chromatography using -50 resin (diameter 7c
m, height 60 cm). The resin was washed with 5 mM potassium phosphate buffer (pH 7.8) in advance, and elution was performed with 0.0015 M potassium phosphate buffer (pH 7.8) to collect the SOD active fraction.

Next, this SOD active fraction was dialyzed against a potassium phosphate buffer (pH 7.8), concentrated with an Amicon concentrator, and this solution was frozen to give purified SO.
D. The protein amount of this purified SOD was measured from the absorbance at 280 nm according to the Lory method to determine the SOD activity value (unit / mg). The results are shown in Table 1. The SOD apotype in the table is obtained by the enzyme inhibition method, and the oxygen partial pressure is shown by the amount of dissolved oxygen in the medium.

[0038]

[Table 1]

Reference Example 2 Using the bacteria shown in Table 2, SOD was prepared in the same manner as in Reference Example 1, and the SOD activity value was determined. The results are shown in Table 2.

[0040]

[Table 2]

Example 1 5% by weight of hexadecyl alcohol (manufactured by Sigma), 35% by weight of silicone oil (KF-96 manufactured by Nacalai Tesque, Inc.), 30% by weight of glycerin (manufactured by Wako Pure Chemical Industries, Ltd.), anhydrous sodium phosphate (Wako Pure Chemical Industries, Ltd.) 3% by weight, carboxymethyl cellulose (Wako Pure Chemical Industries, Ltd.) 17% by weight, glycerin stearate (Wako Pure Chemical Industries, Ltd.) 0.2% by weight, p-
Methyl hydroxybenzoate (manufactured by Wako Pure Chemical Industries, Ltd.) 0.005
%, Kosei (manufactured by Tokyo Kasei) and 0.003% by weight of distilled water were mixed, and the mixture was sufficiently stirred for 5 minutes using a stirrer (manufactured by Nippon Kagaku Kikai Co., Ltd.) to prepare a cream base.

Then, 1 g of this cream base was added to Micrococcus candidas (IAM 1 obtained in Reference Example 2).
2004) -derived Fe-SOD (activity value 9.3 units / m
g) 200 μg was added to prepare a cosmetic for preventing skin aging.

Next, Fe-SOD in the cosmetic composition and Fe-SOD alone are shown in the following in vitro affinity for cultured human epidermal cells, human lymphocytes, human neutrophils and human endothelial cells. Sought by.

That is, each of the cells was suspended in RPMI 1640 medium at a concentration of 10 ⁵ to 10 ⁷ , and the suspension was
Cosmetics containing ¹²⁵ I-Fe-SOD was radiolabeled with ¹²⁵ I and ¹²⁵ I-Fe-SOD alone were added respectively, after 4 hours incubation at 37 ° C., the radioactivity bound to cells, Cosmetics and Fe-SO before addition
The percentage was calculated for each radioactivity of D alone and the affinity was evaluated. The higher this value, the better the affinity.

As for cultured human epidermal cells, 1 cm ² of the normal skin of a burn patient was aseptically collected, and this tissue was washed twice with physiological saline containing kanamycin, and then trypsin and collagenase (both of Chemical Industry Co., Ltd.) was added and digested at 37 ° C. for 30 minutes in a CO ₂ incubator. This tissue was placed on a sponge-like sheet coated with collagen (type IV collagen, manufactured by Seikagaku Corporation), and CO _{2 was} cultivated using a 20% by weight fetal calf serum-containing medium. Regarding human lymphocytes, human neutrophils and human endothelial cells, plastic form fixed with paraformaldehyde was prepared according to a conventional method.
After centrifugation in a polypropylene tube, treatment with ammonium chloride was performed to fractionate each cell, and the cells were collected and used. The results are shown in Table 3. In addition, the numerical value attached with ± in the table is the standard deviation value of three experiments.

[0046]

[Table 3]

As can be seen from Table 3, F in the cosmetic of the present invention
e-SOD has a higher affinity for each cell than Fe-SOD alone.

Example 2 The surface of the skin is subjected to various biological, chemical and physical stresses, and causes cell damage and decrease in cell activity (aging). At that time, more active oxygen than usual may be generated on the skin surface, or the function of removing active oxygen may be reduced. Among such cell disorders, ultraviolet rays have been generally considered as an index of skin disorders. In view of this point, the present example performed the following test.

First, Fe-SO derived from Micrococcus candidas (IAM 12004) obtained in Reference Example 2
A cosmetic for preventing skin aging was prepared in the same manner as in Example 1 using D (activity value 9.3 units / mg).

Next, as a mouse, a male BALB / c (manufactured by CLEA Japan, Inc.) having a body weight of about 30 g at 7 weeks of age was used. The back of the mouse was shaved with an electric clipper, and the cosmetic was applied to the shaved portion. Fe-SOD was applied at 50-150 μg / mouse, and after 30 minutes, a Hitachi ultraviolet lamp (340-400 nm) was used, and ultraviolet rays were emitted at a rate of 2.7 mW / cm ² from a position 15 cm away. Was irradiated over time. Then, take 1 cm ² of skin on the UV irradiation area, and perform flow-offching (Flohe and Otti).
The activity of SOD extracted and purified by the method of ng) was measured. Further, the SOD activity of the skin was measured in the same manner as described above for the control, the one that was irradiated with only ultraviolet rays, and the one that was applied with only the cream base and then was irradiated with ultraviolet rays. The results are shown in Table 4.

[0051]

[Table 4]

As can be seen from Table 4, those irradiated with only ultraviolet rays have a lower skin SOD activity than the control.
On the other hand, the cosmetics of the present invention applied and irradiated with ultraviolet light all have higher SOD activity in the skin than the control.

Example 3 Conventionally, with respect to SOD, there is a problem of allergic reaction between different kinds of organisms and a concern of anaphylactic reaction during long-term use. For cosmetics, single-dose toxicity test, repeated-dose toxicity test, primary skin irritation test, continuous skin irritation test, skin sensitization test, photosensitization test, phototoxicity test, mutagenicity test are conducted, and safety is examined. did. The test method was carried out according to the guidelines of Yakuhin No. 118 of February 15, 1984.

Fe-SOD derived from Aeromonas liquefaciens obtained in Reference Example (activity value 7.0 unit / m
g), Fe-SOD derived from Micrococcus candidas
(Activity value 9.3 units / mg) and Fe-SOD derived from Legionella pneumoniae (activity value 11.7 units / mg)
Were used in the same manner as in Example 1 to prepare three kinds of cosmetics for preventing skin aging A, B and C, respectively.

(1) Single-dose toxicity test Oral and subcutaneous dosing tests were carried out on mice and rats. The LD ₅₀ values of all three cosmetics were 16 g / kg in all tests. It was

(2) Repeated-dose toxicity test Each cosmetic was repeatedly orally administered to mice at an amount of 4, 8, 16 g / kg for 3 months. No deaths were observed, and no macroscopic examination was performed in autopsy cases. Physically and pathologically, the heart, lungs,
No abnormalities in the kidney, liver, stomach, small intestine or large intestine were observed.

(3) Primary Skin Irritation Test A primary skin irritation test (erythema / subcutaneous formation and edema formation) was performed on damaged skin and normal skin. I. The I value was determined. The results are shown in Table 5.

[0058]

[Table 5]

(4) Continuous Skin Irritation Test A guinea pig was administered for 16 days to conduct a continuous skin irritation test. Table 6 shows the determination results.

(5) Skin sensitization test A skin sensitization test was carried out on guinea pigs and observed 24 hours later. Table 6 shows the determination results.

[0061]

[Table 6] In the skin sensitization test, when DNCB was used, erythema + was 3 and edema + was 2.

(6) Photosensitization test A photosensitization test was conducted on guinea pigs to check for erythema.
Observations were carried out after 24 hours and 48 hours. as a result,
Each of the cosmetics A, B and C had a judgment of "-" after 24 hours and after 48 hours.

(7) Phototoxicity test A phototoxicity test was conducted on guinea pigs, and 24 hours later, 4
Observation after 8 hours and after 72 hours was performed. As a result, each of the cosmetics A, B, and C had a judgment of "-" after 24 hours, 48 hours, and 72 hours.

(8) Mutagenicity test A mutagenicity test was carried out at concentrations of 50 μg, 100 μg and 500 μg. As a result, all of the cosmetics A, B, and C were evaluated as “−” in all the concentrations of Rec assay and Ames system.

From the above results, it was confirmed that the cosmetic for preventing skin aging of the present invention has no safety problem.

─────────────────────────────────────────────────── ─── Continuation of the front page (51) Int.Cl. ⁶ Identification code Internal reference number FI technical display area A61K 7/48 // A61K 35/74 D 7431-4C

Claims (3)

[Claims] 1. Hexadecyl alcohol 1-10% by weight, silicone oil 20-45% by weight, glycerin 15-
Superoxide dismutase extracted from bacteria was added to 100 parts by weight of a cream base containing 40% by weight, 0.3 to 7.0% by weight of phosphate and 5 to 25% by weight of carboxymethylcellulose as essential components. As 0.005 to 0.05 parts by weight, as a cosmetic agent for preventing skin aging. 2. The cosmetic for preventing skin aging according to claim 1, which comprises 100 parts by weight of a cream base and 0.01 to 0.03 parts by weight of protein as a proportion of superoxide dismutase. 3. Superoxide dismutase is selected from Pseudomonas, Bacillus, Micrococcus, Aeromonas, Acetobacter, Flavobacterium, Gluconobacter, Lactobacillus, Rhizobium and Legionella. The cosmetic for preventing skin aging according to claim 1 or 2, which is extracted from bacteria.