JPH08208678A - Substance (srt) existing in plant world has basic structure of life, its characteristic eliminates animal cancer (in vivo) and human cancer (in vitro) and it itself degrades naturally - Google Patents

Abstract

PURPOSE: To elucidate basic structure of a life in which a nucleus-like substance/ mitochondria/ribosome in a substance (SRT) derived from a plant are confirmed and specific numbers of main protein components exist in the substance and which has eliminating action on animal cancerous tissue. CONSTITUTION: An extracted solution of a plant is scattered on 10% PDA(potato dextrose agar), incubated in a constant temperature device at 37 deg.C, harvested two weeks after and collected in a screw bottle to give the objective substance (SRT) in which a nucleus-like substance/mitochondria/ribosome in a substance (SRT) derived from a plant are confirmed and six main protein components having 64.47kDa, 51.57kDa, 32.58kDa, 21.50kDa, 17.99kDa and 64.47kDa molecular weight exist in the substance (SRT) and which has pharmacodynamically effective action to permeate cells, destroy and eliminate the cells after administration of SRT in a human cancer (HeLa cell, colon cancer cell) in vitro and to start cavitation after inoculation (intermittent inoculation ten times) to a cancer occurring in the thigh of a rabbit and to eliminate the cancer in an animal experiment in vivo.

Description

Detailed Description of the Invention

Substances widely existing in the plant kingdom (SRT)
However, it has been found that our health maintenance has various medicinal effects. The purpose of the present invention is to study the substance (SRT) through preclinical experiments using protein components of this substance (SRT) and small animals.
It is to confirm the basic structure of T) as a living organism and to characterize its efficacy.

[0002]

2. Description of the Related Art Plants are indispensable for our daily lives, and their uses are diverse. This time, we are limiting what we are looking for to have medicinal effects rather than nutrients. It has been generally known that plant-derived substances are effective as a medicine for medicine, a patch, etc. However, such collection was unique in the process of production, such as being influenced by the climate and having to depend on a special environment. Therefore, it has been directly administered to the human body without undergoing basic experiments and preclinical experiments using small animals. We started to take these things from the basic ones.

[0003]

As described in the section of the prior art, in both the sampling method and the generation method,
Alternatively, even in the preparation method, it is necessary to review such a situation, which has been performed in the presence of peculiarity, to simplify the work process and improve the efficiency. The substance we capture (SRT) meets these requirements.

[0004]

[Means for Solving the Problems] According to each purpose, it is necessary to characterize this substance (SRT) and collect a large amount of it. The basic procedure for culturing this substance is to perform it on an agar medium containing 10% PDA (potato deostrose). Collection is manual, but can be collected efficiently. The substance (SRT) thus collected was also subjected to basic experiments and in vivo experiments, and its pharmacological effect on protein components and animal cancer was solved.

[0005]

ACTIONS AND EXAMPLES The results of the basic experiments of the present invention and the preclinical experiments using small animals are displayed and explained.

[0006]

[First example] Preparation of plant-derived substance (SRT) 1) Material Plant extract 10% PDA (potato dextrose agar), chloramphenicol (25 mg / L) 2) Culture method and conditions Extract on 10% PDA Spread 0.5 ml / Lp Incubate at 37 ° C incubator 2 weeks later and collect in screw bottles (5 ml). 3) Experiments and preservation of plant-derived substances (SRT) 4) Analysis of plant-derived substances (SRT) Structure Membrane wall, nucleoid, mitochondria, ribosome Request to electron microscope facility of Nippon Medical School Analysis of protein components Request to Toyo Rayon Institute of Basic Medicine

[0007]

[Second example] In vitro search for plant-derived substance (SRT) 1) Culture conditions for HeLa and colon cancer cells 10% FCS culture medium a) Powder medium: Nissui Pharmaceutical's MEM medium b) Kanamycin: (strain) ) Nissui Pharmaceutical's anti- substance c) Sodium bicarbonate: Kanto Chemical Co., Ltd. d) Calf serum (Fcs): IRVINE, GIBCO
Company e) Redistilled water 0.25% Trypsina a) 2.5% Trypsin (1: 250): IRVI
2) HeLa and colorectal cancer cell culture medium method 3) Search (Assay)

[0008]

[Third example] Animal bio-retrieval of plant-derived substances (SRT) 1) Materials and management Request from Dr. Satoshi Watari (formerly Tokyo Medical University), Funabashi Farm Co., Ltd. Rabbit: 10 for assay (around 2.0 kg) Vx2 Rabbit (original) 2) Transplant procedure 3) Assay

[0009]

[Effects of the Invention] When the claims are examined on the basis of the above experimental results, substances having the basic structure of these living organisms (SR
Since T) was confirmed to eliminate animal cancer,
It is considered possible that these sick animals can carry on their original lives.

[Brief description of drawings]

Figure 1: Scanning electron microscope
(9000 times) With a shape similar to the sugar confectionery's Konpeito, it has a lot of protrusions

FIG. 2 Scanning electron microscope
(18,000 times)

[Figure 3] Transmission electron microscope
(34000 times) The outer shell has an irregular surface, and cell walls with high electron density are seen.

FIG. 4 Transmission electron microscope
(60000 times) Cell membrane, cytoplasm and ribosome are seen

FIG. 5 Transmission electron microscope
(12000 times) nucleoid and cytoplasm are seen

FIG. 6 Transmission electron microscope
(40,000 times) Cell membrane, cytoplasm, nucleoid, ribosome, mitochondria are found

FIG. 7: Incorporation of SRT substance into cultured HeLa cells

FIG. 8: Uptake of SRT substance into colon cancer cells

FIG. 9: Behavior of SRT on cultured cells (HeLa) (18000 times)

FIG. 10 SR for the surface of cultured cells (HeLa)
Implantation of T (28,000 times)

FIG. 11: Expansion of implantation of SRT on cultured cells (HeLa) (42000 times)

FIG. 12: Invasion behavior of SRT to cultured cells (HeLa) (36000 times)

FIG. 13: Attack of SRT on cultured cells (HeLa) (excretion of mucus) (20,000 times)

FIG. 14: Attack of SRT on cultured cells (HeLa) (excretion of mucus) (60000 times)

FIG. 15: Destruction of cultured cells (HeLa)
(60000 times)

FIG. 16: Electrophoresis of protein component of SRT substance

FIG. 17: Measurement of animal cancer (rabbit thigh)

FIG. 18 Peeling of body surface of animal cancer (thigh of rabbit)

FIG. 19: Removal of animal cancer

FIG. 20: Surface layer of excised animal cancer

FIG. 21: Inner layer of animal cancer

FIG. 22: Removal of active tissue part of Vx2 of animal cancer

FIG. 23: Repeated washing centrifugation after homogenization of animal cancer

FIG. 24 Animal cancer tissue fluid

FIG. 25: Transplantation of new animal (rabbit) into thigh

FIG. 26 Transplantation

[Fig. 27] Rabbit dissection (confirmation of thigh and cancerous transformation)

FIG. 28: Cavitating phenomenon by SRT for cancer of rabbit thigh

Figure 29: Invasion of hollowing out

FIG. 30: Disappearance of animal cancer (rabbit thigh) (about 80 to 90%)

[Fig. 31] Phenomenon of hollowing (20-30%)

FIG. 32: Phenomenon of hollowing (60 to 70%)

FIG. 33 Phenomenon of reduction of animal cancer (rabbit thigh) (unit: cm) (1.7 × 2.5 × 2.0)

FIG. 34 Phenomenon of reduction of animal cancer (thigh of rabbit) (unit: cm) (1.7 × 2.5 × 2.0)

FIG. 35: Dissection of rabbit femoral cancer tissue part

FIG. 36: Dissection of rabbit femoral cancer tissue part

FIG. 37: Electron microscope (40000 ×) of SRT behavior on rabbit femoral cancer tissue

FIG. 38: Electron microscope (26400 ×) of SRT behavior on rabbit femoral cancer tissue

FIG. 39: Electron microscopy of SRT behavior on rabbit femoral cancer tissue

FIG. 40: Electron microscope (30000 ×) of SRT behavior on rabbit femoral cancer tissue

FIG. 41: Electron microscope (48000 ×) of SRT behavior on rabbit femoral cancer tissue

FIG. 42: Electron microscope (36000 ×) of SRT behavior on rabbit femoral cancer tissue

FIG. 43: Electron microscope (48,000 ×) of SRT behavior on rabbit femoral cancer tissue

FIG. 44: Destruction disappearance by rabbit femoral cancer tissue SRT (40,000 times)

FIG. 45: Electron microscope (28000 ×) of SRT behavior on rabbit femoral cancer tissue

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[Procedure amendment]

[Submission date] July 3, 1995

[Procedure Amendment 1]

[Document name to be amended] Statement

[Name of item to be amended] Title of invention

[Correction method] Change

[Correction content]

The substance existing in the plant kingdom (SRT) has a basic structure of living organisms, and its characteristic is animal cancer (in vi
vo) and human cancer (in vitro) are eliminated. In addition, it itself collapses naturally.

[Procedure Amendment 2]

[Document name to be amended] Statement

[Name of item to be corrected] Brief description of the drawing

[Correction method] Change

[Correction content]

[Brief description of drawings]

Figure 1: Scanning electron microscope
(9000 times) With a shape similar to the sugar confectionery's Konpeito, it has a lot of protrusions

FIG. 2 Scanning electron microscope
(18,000 times)

[Figure 3] Transmission electron microscope
(34000 times) The outer shell has an irregular surface and cell walls with high electron density type are seen

[Figure 4] Transmission electron microscope
(60000 times) Cell membrane, cytoplasm and ribosome are seen

FIG. 5: Transmission electron microscope
(12000 times) nucleoid and cytoplasm are seen

FIG. 6 Transmission electron microscope
(40,000 times) Cell membrane, cytoplasm, nucleoid ribosome, mitochondria are found

FIG. 7: Uptake of SRT substance into HeLa cells (light microscope: in vitro)

FIG. 8: Uptake of SRT substance into colon cancer cells (light microscope: in vitro)

FIG. 9: Behavior of SRT on cultured cells (HeLa)
(18,000 times)

FIG. 10: SRT on the surface of cultured cells (HeLa)
Implantation (28,000 times)

FIG. 11: Expansion of implantation of SRT on cultured cells (HeLa) (42000 times)

FIG. 12: Invasion behavior of SRT to cultured cells (HeLa) (36000 times)

[Fig. 13] Attack of SRT on cultured cells (HeLa) (emits mucus) (2000 times)

FIG. 14: Attack of SRT on cultured cells (HeLa) (to release mucus) (60000 times)

FIG. 15: Electron micrograph (× 60000) of a phenomenon in which cultured cells (HeLa) are destroyed.

FIG. 16: Photograph of electrophoresis of protein component of SRT substance

FIG. 17: Measurement of animal cancer (rabbit thigh)

FIG. 18: Detachment of body surface of animal cancer (thigh of rabbit)

FIG. 19: Removal of animal cancer

FIG. 20: Surface layer portion of excised animal cancer

FIG. 21: Inner layer of animal cancer

FIG. 22: Removal of active tissue part of Vx2 of animal cancer

FIG. 23: Repeated washing centrifugation after homogenization of animal cancer

FIG. 24: Animal cancer tissue fluid

FIG. 25: Transplantation of new animal (rabbit) into thigh

FIG. 26: Rabbit dissection (confirmation of thigh and cancer tissue site)

FIG. 27: Cavitating phenomenon by SRT for cancer of rabbit thigh

FIG. 28: Disappearance of animal cancer (rabbit thigh) (about 80 to 90%)

FIG. 29: Phenomenon of hollowing (20-30%)

FIG. 30: Phenomenon of hollowing (60 to 70%)

FIG. 31 Phenomenon of reduction of animal cancer (thigh of rabbit) (unit: cm) (1.7 × 2.5 × 2.0)

FIG. 32: Phenomenon of reduction of animal cancer (rabbit thigh) (unit: cm) (1.7 × 2.5 × 2.0)

FIG. 33: Dissection of rabbit femoral cancer tissue part

FIG. 34: Dissection of rabbit femoral cancer tissue part

FIG. 35: Electron microscope (40,000 ×) of SRT behavior on rabbit femoral cancer tissue

FIG. 36: Electron microscope (26400 ×) of SRT behavior on rabbit femoral cancer tissue

FIG. 37: Electron microscopy of SRT behavior on rabbit femoral cancer tissue

FIG. 38: Electron microscope (30000 times) of SRT behavior on rabbit femoral cancer tissue

FIG. 39: Electron microscope (48000 ×) of SRT behavior on rabbit femoral cancer tissue

FIG. 40: Electron microscope (36000 ×) of SRT behavior on rabbit femoral cancer tissue

FIG. 41: Electron microscope (48000 ×) of SRT behavior on rabbit femoral cancer tissue

FIG. 42: Destruction disappearance by rabbit femoral cancer tissue SRT
(40,000 times)

FIG. 43: Electron microscope (28000 ×) of SRT behavior on rabbit femoral cancer tissue

[Procedure 3]

[Document name to be corrected] Drawing

[Correction target item name] All drawings

[Correction method] Change

[Correction content]

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Claims (4)

[Claims] 1. Nucleoid bodies, mitochondria, ribosomes, etc. were confirmed in the substance (SRT), and there were 6 main protein components in the substance, and 4 were confirmed except when the previous application was filed. 2. The molecular weight of the protein component is the third 32.58 kDa, the fourth 21.50.
5th kDa 17.99kDa 6th 1
It is 4.79 kDa. (However, the first one in the last time was 64.
47kDa second is 51.57kDa) 3. In vitro tests, in human cancers (HeLa cells, colon cancer cells), enter SRT after administration of SRT and destroy and destroy the cells. 4. In vivo experiments in animals, the cancer (derived from Vx2) that has developed in the thigh of the rabbit begins to disappear after disappearing after inoculation of SRT (10 inoculations in brief).