JPH0818928B2 - Deodorized formalin - Google Patents

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to formalin having no offensive odor or irritating odor.

[0002]

BACKGROUND OF THE INVENTION Formalin is used not only as an industrial material for producing synthetic resins, but also as a sanitary material such as a sterilizer and a disinfectant, and as a sample fixative in the field of histopathology. Its field of use is extremely wide.

However, formalin has a peculiar offensive odor and irritating odor, and particularly when it is used as an antiseptic or a disinfectant, a strong irritating odor is generated, which is a problem.

Therefore, the present inventor has previously described that the characteristic malodor and irritating odor of formalin as described above are removed while maintaining the original characteristics of formalin.
The inventor of the deodorized formalin disclosed in Japanese Patent No. 45502, which is a mixture of formalin with methyl salicylate and ethanol or methanol.

[0005]

DISCLOSURE OF THE INVENTION The present invention further improves the above deodorized formalin to provide less deodorized formalin which is less irritating and does not impair the original properties of formalin and is more excellent as a sample fixative. It is what

[0006]

In order to achieve the above-mentioned object, the deodorized formalin according to the present invention comprises, for the purpose of achieving the above objects, methyl salicylate, ethanol or methanol, eucalyptus oil or hinokitiol , and first phosphorus. It is a mixture of sodium phosphate, dibasic sodium phosphate, and calcium chloride or calcium acetate.
In particular, in the above case, 9.9 ml of formalin
0.1 ml of methyl salicylate and 0.7-0.8 ml of ethanol or methanol mixed with eucalyptus oil or hinokitiol of 0.3-0.
8% by weight, 0.43 to 0.47 of monobasic sodium phosphate
% By weight, 0.64 to 0.70% by weight of dibasic sodium phosphate, 0.1% of calcium chloride or calcium acetate.
It is mixed at a ratio of 0 to 0.20% by weight.

[0007]

The deodorizing formalin according to the present invention as described above is
Methyl salicylate masks the malodor and irritating odor peculiar to formalin, and the alcohol component prevents the decomposition of aldecht to improve the preservability of this deodorized formalin.
This alcohol component improves the solubility of eucalyptus oil, which will be described later, in formalin, and produces deodorized formalin having no bad odor or irritating odor without impairing the original properties of formalin.

In addition to the above, in the present invention, eucalyptus oil, hinokitiol and other irritating tree oils are added, whereby these eucalyptus oil and hinokitiol more completely suppress the malodor and irritating odor of formalin, Use mild formalin.

The monosodium phosphate and the dibasic sodium phosphate act as a buffering agent, and keep the pH of the deodorized formalin in the range of 7.2 to 7.4.

Further, calcium chloride or calcium acetate acts as a buffering aid, and by adding it, the deodorized formalin becomes more suitable as a fixing solution for enzymes, lipids, mucopolysaccharides and the like.

[0011]

[Examples] To 990 ml of formalin, 10 ml of methyl salicylate, 7 to 8 ml of ethanol, and 5 ml of eucalyptus oil were added at a ratio of 4.56 g of sodium monophosphate and 6.67 g of dibasic sodium phosphate. 1 g of calcium chloride was added and completely dissolved to obtain deodorized formalin.

This deodorized formalin is a colorless and transparent liquid having a pH of 7.2, has no bad odor or irritating odor, and has the same characteristics as ordinary formalin in terms of sterilization, antibacterial effect, specimen fixing effect and the like. It was something that had.

(Experiment 1) Using the deodorant formalin according to the present invention, a rat organ was fixed and its fixing effect was examined.

Test method Animal: SD rat (9-week-old male, 2 animals) Target organ: liver, kidney, spleen, brain Organ size: 5 mm cubic Fixative solution: 12.5 ml (100 organ volume) Fixing time: 24 hours at room temperature Dehydration, embedding: After passing through ethanol-increasing series and xylene (using an automatic embedding machine), embedding in paraffin Embedding: Staining with 3-4μ using a Jung-type microtome Method: 1. Hematoxylin / eosin staining 2. Periodic acid chef staining 3. Azan Mallory staining 4. Silver transfer (Watanabe method) Target fixatives: The following two fixatives were used as target fixatives. 1. 10% neutral buffered formalin solution (formulation) formalin stock solution 100 ml sodium monophosphate 4.4 g dibasic sodium phosphate 6.5 g distilled water 900 ml 2. formalin / ethanol solution formalin stock solution 200 ml methanol 200 ml distilled water 600 ml

Test Results As a result of the above test, the deodorized formalin according to the present invention is equivalent to the neutral buffered formalin solution or the formalin / methanol solution as the target fixative solution in terms of dyeability and tissue fixability. Or even more.

(Experiment 2) Further, the deodorized formalin according to the present invention was examined for its gas state and sterilization effect as formalin water.

Test method 1. Test bacteria: Bacillus subtilis 2. Sterilization test method 1) Method using formalin water The deodorized formalin according to the present invention and the formalin diluted to 3.7% as the target solution were used as stock solutions, and these 2
Each diluted solution of x, 3x, 4x, and 5x was prepared. 10 ml of each of the diluted solution, stock solution and target solution was placed in a sterilized medium test tube with a cotton plug, and 1 ml of the bacterial solution of Heart infusion (Heat infusion) broth separately cultured (37 ° C, 24 hours) was placed in each tube. In addition, place this test tube at 20 ° C for 60 minutes, 90 minutes, 120
After keeping for 1 minute, 180 minutes, and 240 minutes, take 1 platinum loop of bacterial solution from each test tube and inoculate into a new broth.
The viability of the fungus was determined after a lapse of time. 2) Method using formalin gas
Incubate for 24 hours at 37 ° C on the slope of a fusion agar), pick up 1 platinum loop of this moss, apply it to sterile gauze (1 x 1 cm) or sterile absorbent cotton (1 x 1 cm), and put it in a sterile petri dish. It was Separately, 50 ml of a 19% diluted formalin formalin or the deodorizing formalin according to the present invention was placed in the bottom of a desiccator (internal volume: 1100 cm ³ ), and 50 ml of water was placed in the target desiccator. On the shelves of these desiccators, a petri dish containing the above-mentioned gauze moistened with the above fungi or absorbent cotton was placed, and petroleum jelly was applied to the desiccator lid to seal it. Keep this desiccator in a 37 ° C incubator for 7 hours, 24 hours, 48 hours, 72 hours.
After keeping it for a certain period of time, it was taken out from the incubator, and gauze or absorbent cotton with fungus was placed in 10 ml of fresh broth and cultured at 37 ° C. for 48 hours to determine the viability of the fungus.

Test Results As a result of the above test, the deodorized formalin according to the present invention can be used in any state of formalin water or formalin gas.
It showed a sterilization effect equivalent to that of the pharmacopoeial formalin.

[0019]

Industrial Applicability As described above, the deodorized formalin according to the present invention has the original properties of formalin, has no malodor or irritating odor peculiar to formalin, and has no problem in spraying and use in a gas state. , Due to the effect of the buffer, P
It is a neutral buffered deodorant formalin that can be used as a fixative in any sample fixing method such as light microscopy, enzyme histochemistry, fluorescent antibody method, immunohistochemistry method, etc., by holding H in the neutral range.

Claims (4)

[Claims] 1. A pharmacopoeial formalin, methyl salicylate,
Ethanol or methanol, and eucalyptus oil
Deodorized formalin, characterized by being mixed with cucurbit hinokitiol . 2. Pharmacopoeia formalin, methyl salicylate,
Ethanol or methanol, eucalyptus oil or
Deodorized formalin obtained by mixing and dissolving nokithiol , monobasic sodium phosphate and dibasic sodium phosphate. 3. A pharmacopoeial formalin, methyl salicylate,
Ethanol or methanol, eucalyptus oil or
A deodorized formalin characterized by being obtained by mixing and dissolving nokithiol , sodium monophosphate, dibasic sodium phosphate, and calcium chloride or calcium acetate. 4. Eucalyptus in a mixture of 9.9 ml of pharmacopoeial formalin with 0.1 ml of methyl salicylate and 0.7 or 0.8 ml of ethanol or methanol.
0.3 to 0.8% by weight of oil or hinokitiol, 0.43 to 0.47% by weight of monobasic sodium phosphate, second
Sodium phosphate 0.64 to 0.70% by weight, calcium chloride or calcium acetate 0.10 to 0.20
The deodorized formalin according to claim 3 , wherein the deodorized formalin is mixed in a ratio of wt%.