JPH08134449A - Liquid fermentation soil activator - Google Patents

Abstract

PURPOSE: To obtain a liq. fermentation soil activator which is useful for fertilizing soil, improving soil structure, and improving the growth and qualities of cultivated plants by using, as the main effective component, a symbiotic mixed culture product of a specific thermophilic actinomycete and another kind of fibrin-decomposing bacteria. CONSTITUTION: The liq. fermentation soil activator contains, as the main effective component, a symbiotic mixed culture product comprising a thermophilic actinomycete, thermoactinomyces SKO53 sp. nov. (Agency of Industrial Science and Technology, Life Engineering Research Institute, FERM P-13598), which belongs to the genus thermoactinomyces and has a growth temp. range of 10-85 deg.C, a growth pH range of 5.3-10.8, and lignin-solubilizing and fibrin- decomposing capabilities and another kind of thermophilic fibrin-decomposing bacteria, Clostridium thermocellum biovar. SK522 (Fermentaion Research Institute FERM BP-3459), which has a lignin-solubilizing capability and a growth temp. range of 65-72 deg.C and causes a vigorous fermentation of fibrin. A mixed culture with methanogens, hydrogenomonas, or aerobic, anaerobic, or thermophilic fibrin-decomposing bacteria is also favorable.

Description

Detailed Description of the Invention

[0001]

[Industrial application] A new fungus that is useful for promoting fermentative ripening of organic substances to fertilize soil, improve soil structure, and at the same time improve the growth and quality of cultivated plants and technology for effective use of the new fungus Regarding The liquid fermenting soil activator is a solution fertilizer (Mizugoe) obtained by solution-fermenting and aging an animal or plant organic substance with microorganisms, and specifically, seafood, livestock meat, leather, animal horns. , Mizuhoe of hoof (Sakura fertilizer), Lusan and Tsurumurasaki, other green grass, fruits, etc. are liquid fermented.

[0002]

[Prior Art] Although it uses difficult and strict words such as liquid fermenting soil activator, in Japan, "Mizugoe" has always been used.
It was used by farmers as "exposed". The material is weeds or kitchen garbage. However, the development of modern agriculture and Japan are no exception. Excessive integration, chemicalization, large scale, single cropping, etc. have led to a decline in soil fertility, and continuous crop failures and soil diseases have become major problems, causing groundwater pollution. However, along with this, interest in organic farming and soil microorganisms has increased, and attention has been paid to the Japanese traditional "blur" and "Mizugoe", and their application effects have come to be expected. However, both the research and the technology, the "fermentation or spoilage microorganism itself, its physiology and mechanism of action", which is the core of the research and technology, are discussed as "black box". In addition, it is difficult to understand the appearance of all microorganisms during these ripening processes, especially the function of effective microorganisms, because the ecological handling of the microflora in which the substrate is locally accumulated has not been established. Is.

[0003]

Under these circumstances, the present applicant pursues these aging processes from the standpoints of plant physiology / biochemistry and rhizosphere microorganisms, especially microbial ecology, and aging of these fermentation rots. Of microbial groups involved in Transformation of dominant microflora during ripening process and its biologic examination. Main microorganisms and their effects Production of a liquid fermenting soil activator that serves as a standard under the conditions in the test field. Measurement of changes in main ingredients of raw materials during the aging process. An index of reasonable maturity. Relationship between prevention of growth disorders of natural raw materials and disease and pest damage and identified microorganisms At the same time as trying to elucidate the above-mentioned problems, the results of the test and research are the results of screening of some effective bacteria and substance decomposition by symbiotic culture. And the probability of technology, and the provision of a new liquid fermenting soil activator consisting mainly of a new thermophilic actinomycete.

[0004]

[Means for Solving the Problems] The constitution of the present invention, which has solved the above-mentioned problems, is a novel thermophilic actinomycete Thermoactinomyces SK053 sp. Nov. (Thermoactinomyces) described in 1 below.
SK053 sp.nov., Institute of Biotechnology, Industrial Technology Institute
Accession number FERM P-13598) and the new thermophilic fibrinolytic bacterium Clostridium thermocellum SK522 (Clostridium thermocellum biovar.SK) described in 2 below.
522, Mikori Kenjoyori No. 3459), which is a liquid fermenting soil activator containing as an active ingredient a symbiotic mixed culture.

According to the present invention, the strain SK053 and SK52
It is preferable to perform mixed culture in addition to the two strains. Other fibrin-degrading bacteria include aerobic, anaerobic, thermophilic, and physiologically fermenting fibrin, and particularly methane bacteria that generate a large amount of methane gas, or hydrogen bacteria that generate hydrogen. Specifically, there are the following.

(1) Aerobic fibrin-decomposing bacteria (suitable temperature 22-35)
℃) Bacillus fimi (Cellulomonus fumi), others genus Cel
lulomonus, Cellfalcicula viridis, C.mucosa Actinomyces melanocyclus (Micrococcus melanocyclu
s), other genus Actinomyces Cellulyticum flavum Celluvibrio flavescens, other genus Celluvibrio Cytophaga hutchinsonii, other genus Cytophaga Pseudomonas fibrolysis Methanobacterium soehngenii, M.omelianskii Sarcina methanica

(2) Anaerobic fibrin-decomposing bacteria (suitable temperature 25-37
℃) Clostridium werneri Plectridium cellulolyticum

(3) Thermophilic fibrin-decomposing bacteria (suitable temperature 55-65
℃) Clostridium thermocellum, C.thermocellulaseum Bacillus cellulosae dissolvens, B.thermofibrincol
us

(4) Bacterium cellaresolvens, a denitrifying fibrin-degrading bacterium (35 ° C)

As used in the specification, the hardly decomposable organic material or the hardly decomposable fiber material means sawdust, chip dust, planer refuse, bark (bark) and other waste materials in the wood industry, straw rods such as rice husks, rice straws and wheat straws. , Soybean, azuki bean, fallen seeds and soybean seed coats and pods, coffee grounds, deciduous leaves, bark, mountain grasses such as reeds and kaya, as well as abolished mushrooms such as shiitake mushrooms and other mushrooms 100
The above-mentioned various persistent plant bodies. The organic components that make up these plant remains are extremely complex and diverse, but generally, the main component is fibrin, which is about 30-75.
%, And then 15 to 40% of lignin and 45 to 90% or more when both are combined, and occupy most of them. Next is hemicellulose in the order of 7 to 25%, and in a small amount, nitrogen-containing compounds such as proteins, various sugars, organic acids, alcohols, and fats, waxes, essential oils and the like are contained.

The hard protein referred to in the specification is water,
Proteins such as collagen, keratin, otscein, and fibroin that are insoluble in salt solutions, light acids, and light alkalis, are not easily hydrolyzed by acids and alkalis, and are not easily affected by enzymes, such as bones and teeth, horns, nails, and hair. It is the main component.

[0012] The excipients used in the present invention are limestone rock powder, dolomite rock powder, shell fossil powder, crab shell, shellfish shell powder such as shellfish, calcium carbonate, slaked lime, perlite,
There are powder particles such as vermiculite, zeolite, diatomaceous earth, basic rock rock powder, ground peat moss, charcoal and charcoal powder, and one kind or a mixture of plural kinds thereof is shown.

The micronutrients and trace minerals of the present invention include vitamins such as biotin, nicotinamide, thiamine, pyridoxamine, vitamin B12, balaaminobenzoic acid, arginine, cystine, glutamic acid, isoleucine, leucine, proline, lysine and methionine. , Phenylalanine, threonine, tryptophan, tyrosine, valine, and other amino acids, and iron, manganese, copper, cobalt, calcium, magnesium, and other trace minerals. As described above, these excipients, micronutrients and trace minerals are appropriately selected according to effective bacteria, purpose of use, soil environment, climate and climate. The thermophilic actinomycete of the present invention, Thermoactinomyces SK053 sp. Nov.
yces SK053 sp. nov., Institute of Biotechnology, Institute of Biotechnology, Accession No. FERM P-13598) is hereinafter simply referred to as a new strain SK053 or SK053 strain.

[0014]

The present invention provides a useful new bacterium, SK053.
The present invention also relates to a mixed culture of the SK053 strain and the SK522 strain and its effective use. The synergistic effect between the new bacterium SK053 and the SK522 strain enhances not only the remarkable solubilizing ability of lignin but also the decomposition ability of fibrin and other functions. This can be a powerful liquid fermentation in-process activator.

In particular, the new strain thermophilic fibrinolytic bacterium Clostridium thermocellum SK522 (Clostrid
uim thermocellum biovar.SK522, Micro Engineering Lab. No.3
No. 459) (hereinafter abbreviated as SK522 strain) and thermophilic mixed culture (60 to 65 ° C.) are not an additive action of the functions of both strains but a synergistic effect caused by the interaction. , Mutually enhance their functions, ferment strongly and stably various natural hard-to-decompose fiber materials and hard proteins, which are considered to be the most difficult, and quickly and rapidly produce lignin, fibrin, proteins, hemicellulose and other organic components. Strongly decomposes and digests.

By using the mixed culture of the present invention as an active ingredient, liquid fermenting soil activators of various organic materials can be obtained. Liquid fermenting soil activator of these organic materials,
Depending on the use, purpose, climate, climate, soil composition, etc., the new strain SK053, in addition to the selected fibrin-degrading bacteria, can be mixed with useful microorganisms to further enhance the effect.

[0017]

EXAMPLES The present invention and examples of use thereof will be described in detail below. The scientific properties of the novel bacterium SK053 of the present invention are as follows.

Thermoactinomyces SK053 sp. Nov. I. Thermoactinomyces SK053 sp. Scientific properties Morphological characteristics Heat-resistant true endospore formation, forming single spores in basal hyphae and aerial hyphae. Spores are smooth, spherical, diameter 0.4 to 0.7
μ. Lacking or very short spore stalk. Basal hyphae are non-dissociative and have a diameter of 0.5μ. Very vigorous growth on agar medium, promptly forming aerial hyphae, white and powdery. Gram positive. Cultural properties Growth is extremely active. Colonies are formed in 4 to 6 hours and covered with white aerial hyphae. (1) Plate culture In addition to nutrition, yeast / malt, oat mill, sucrose / nitrate, glucose / asparagine, glycerin /
60 ° C culture of synthetic agar medium (3%) such as asparagine and tyrosine is very good and forms aerial hyphae. The colony surface has a white flora. The surface and back of the mycelium are colorless. There is no production of diffusible dye in the medium. (2) Liquid culture Meat static culture: A white film is formed. Often forms compact precipitates. Milk culture: coagulation, peptonization. Physiological and biochemical properties This bacterium markedly enhances solubilization of lignin by symbiotic mixed culture with various thermophilic, mesophilic, psychrophilic or anaerobic, aerobic or denitrifying fibrinolytic bacteria. At the same time, it also has a positive effect on the fibrin resolution. The water-soluble low-molecular-weight or decomposed substance produced in this way is directly absorbed and utilized by the plant or promotes the growth of soil-effective bacteria to establish a healthy and vigorous plant rhizosphere microbial ecosystem. And
As one of the pathways of carbon cycle in agricultural systems and natural ecosystems, the decomposition and humification of hard-to-decompose natural fiber materials is carried out strongly, stably and surely. 1. Enzyme action, etc. (1) Proteolytic enzyme (Proteolytic enzyme): Positive (strong) Gelatin hydrolysis: Positive Peptidase: Positive (2) Starch hydrolysis: Positive (3) Pectin degradation: Negative (4) Degradation of fibrin: positive (weak) (5) Degradation of lignin: positive Degradation of fibrin and lignin is both positive but weak. However, symbiotic mixed culture with fibrinolytic bacteria strongly solubilizes lignin and at the same time enhances fibrin degradability. (6) Lipolysis: Negative (7) Invertase: Negative (8) Catalase reaction: Positive (9) Okinidase reaction: Positive 2. Product test (1) Raw acid and gas generation from sugar and alcohol No gas is generated. (2) Pigment production: Formation of melanin-like pigment (tyrosine and peptone yeast iron agar): negative 3. Growth conditions (1) Growth temperature Optimum temperature 25 to 78 ℃, growth temperature range 10 to 85 ℃, the growth range is also very wide, and it has characteristics not found in other Thermoactinomyces species. (2) Hydrogen ion concentration Optimum hydrogen ion concentration pH = 6.5 to 9.6. Growing hydrogen ion concentration range pH = 5.3 to 10.8. This is also one of the characteristics not found in other species of the same genus. (3) Assimilation of nitrogen sources Organic and inorganic nitrogen sources are often used. (4) Assimilation of carbon source (on Pridham-Godlieve agar medium) D-glucose, sucrose, cellobiose, maltose, starch, organic acid, and glutamate are used. (5) Relationship with oxygen Absolutely aerobic. (6) Requirement of micronutrients In addition to biotin, thiamine, nicotinic acid, etc., flavonoid pigments such as rutin and hespyridine (Flav
Micronutrients such as onoid pigment are required. Furthermore, relatively high concentrations of ions such as phosphate and potassium are required for good growth of this bacterium, and the presence of carbon dioxide gas
Required for its growth and germination of spores. (7) Harmfulness to health or environment It is said that there are some pathogenic strains of bacteria belonging to this genus.
As far as this strain is concerned, I do not know that it has such properties. Separation sources Mainly high-speed high-temperature compost, aged muck / manure, agricultural soil, etc. Content of G + C of DNA mol% of G + C = 52.7 to 54.2 (Tn).

II. Taxonomic position The International Committee on Syste
Approval list of bacteriology names edited by matic Bacteriology (ICSB) (1980) and edited by Takeharu Hasegawa (Yoshiro Okami,
Seino Akio): Classification and identification of microorganisms (bottom), p. 1-92
(1985), Berger's Manual (Berg
ey's Manual of Determinative Bacteriology 7 ed., 1
957; 8ed., 1974; Bergey's Manual of Systematic
Bacteriology vol.1, vol.2, 1984, 1986.) was performed as a reference. As a result, Thermoactinomyces (genus
Thermoactinomyces)). The classification criteria in Burger's manual, which is generally used for the search and identification of bacteria and actinomycetes, cannot be said to be perfect, and there are many uncertainties. Especially for actinomycetes genus Thermoactinomyces (genus Thermoactinomyces) Even the categorical affiliation is ambiguous. The same manual (198
According to 6), it is very similar to the genus Bacillus, and this genus belongs to other actinomycetales.
) Is excluded because it has a low phytophilicity, but it is not included in the family of Bacillaceae.
In addition, the Genus Thermoacti
There are 3 species belonging to nomyces) in the Burger's manual (7th edition, 1957) and 3 in the same manual (8
Edition, 1974), 5 types, and the same systematic (2
Vol. 1986), Type species T. vulga
Only one species of ris is listed. Also, in other classification and identification documents, the number of species to which they belong varies, Nonomura, Ohara et al. (1) has 3 species, and ICSB has so far been the reference species Th.
ermoactinomyces vulgarisTsiklinsky 1898, Type stra
in: In addition to KCC A-0162, 5 types are approved names (nomen approb
atum). Actinomycetes,
The classification criteria of Order Actinomycetales are slightly different from those of other bacteria. For example, actinomycetes are characterized as a treasure trove of useful physiologically active substances such as antibiotics, and the classification of actinomycetes has made rapid progress in recent years in parallel with the remarkable recent development of antibiotics research. . That is, comparison of DNA base composition and sequence, DNA
In addition to genetic biochemical methods, which mainly consist of hybridization (DNA-hybridization) tests and other molecular-level analysis, serological immunological classification, classification by phage sensitivity, classification by constituent sugar and lipid composition of bacterial cells, whole bacteria From the idea that the classification by the IR spectrum of is attempted, and at the same time, the development of electronic computing equipment will simultaneously create a highly objective classification system.
Numerical taxonomy and other methods have been tried, and so-called new methods of chemical taxonomy (Chemotaxonomy) have been introduced rapidly. However, the introduction of these new findings into actinomycetes and bacterial taxonomy is in part, largely based on traditional classical taxonomy, based on characteristic phenotypic traits. It is not an exaggeration to say that we are in a very transient period. Therefore, at the present time, this actinomycete has been determined to be a new species, and Thermoactinomyces
SK053 sp. Nov. (Thermoactinomyces SK053 sp. Nov.)
Is displayed. Finally, a comparison of the main scientific properties that should be mentioned in the taxonomy between this actinomycete and the reference species of the genus Thermoactinomyces Thermoactinomyces brigaris is shown below (see Table 1). Document (1) Hideo Nonomura, Takeshi Ohara: Journal of Fermentation Engineering, 49, 8
95 (1971).

[Table 1] Description of Thermoactinomyces bulgaris (T. vulgaris), a reference species of the genus Thermoactinomyces in Table 1 (descriptio
n) is Tiskrinsky (Tsikl) about 100 years ago
insky). Therefore, there is no development of instrumental analysis techniques and microanalysis as at present, and there is no consideration for chemical taxonomy or consideration for production of physiologically active substances such as antibiotics. Under such an historical background, a comparative examination of the scientific properties of the present strain and the reference strain, including closely related strains, revealed the following characteristic differences, and the Thermoactinomyces genus (genus Thermoacti
nomyces) and a new strain.

(1) Heat-resistant genuine endospores are formed one by one on the basal hyphae and aerial hyphae. It has a high growth temperature and is highly thermophilic, and it can be definitely identified as the Thermoactinomyces genus, but it is very different from the reference species in that it has an optimum growth temperature of 25 to 78 ° C.
In the same temperature range of 10 to 85 ° C, it is much higher than the reference species and also extremely low. The temperature range for growth is generally thermophilic, mesophilic, and low temperature. (2) Optimum hydrogen ion concentration for growth pH = 6.5-9.
6, concentration range pH = 5.3 ~ 10.8 and acidic, neutral,
It has a wide range of alkalinity and concentration, and is strong on the alkaline side. (3) The enzyme activity of the reference species is positive for both gelatin solubilization and starch hydrolysis, but there is no description of negative fibrin degradation or lignin solubilization. Although there is a description that fibrinolysis is positive in closely related bacteria, it is very weak and cannot be used for industrial production. Further, there is no description about the enzyme activity for lignin. Therefore, the ability of this bacterium to decompose fibrin and the ability to solubilize lignin is the second characteristic of this bacterium. Then, by symbiotic mixed culture of this bacterium with various thermophilic, mesophilic, psychrophilic, or anaerobic, aerobic, or denitrifying fibrin-degrading bacteria, the solubilizing ability of lignin is significantly enhanced, At the same time, it has a positive effect on the fibrin resolution. This function of this bacterium
From the point of particular emphasis of the present inventor, the discovery of this peculiarity is a key idea of the present invention. In addition, this bacterium has a strong proteolytic activity and decomposes hard proteins well.

(4) In addition to biotin, thiamine, nicotinic acid and the like, micronutrients such as flavonoid pigments such as rutin and hespyridine are required for vigorous growth of the bacterium. (5) The bacterium is absolutely aerobic, Gram-positive, positive for catalase reaction and oxidase reaction, and G + of DNA
Mol% of C = 52.7 to 54.2 (Tn),
It is less closely related to other actinomycetes and is a genus Bacillus (genus Ba
This is the reason why it has a strong affinity with cillus).

For this reason, the specificity of the growth temperature of the bacterium and the physiological and biochemical properties of the lignin-solubilizing ability of the bacterium were determined to be a new species as one basis, and Thermoactinomyces SK053sp.nov. (Thermoactinomyces SK0
53 sp.nov.).

Using this strain as a reference strain, among the strains belonging to the genus Thermoactinomyces,
It has a lignin solubilizing ability, and is characterized by significantly increasing the lignin solubilizing ability by symbiotic mixed culture with a fibrinolytic bacterium, and includes a physiology biochemical including SK053 strain and natural and artificial mutants. It is a new bacterial species depending on its properties.

Microorganism accession number The microorganism accession number of this bacterium is the accession number FERM P-13598 of the Institute of Biotechnology, Institute of Industrial Science and Technology.

Screening of New Bacterium SK053 Mainly, high temperature and high speed compost, matured manure / manure, agricultural soil, etc. are used as a separation source and agar plate culture is performed at 55-60 ° C. to perform separation according to a standard method. MY medium is used as the medium. It is advisable to add micronutrients as needed.

Utility of new bacterium SK053 The new bacterium SK053 originally appears as a white, powdery microorganism in high-quality compost, or fully matured compost and manure. The appearance of this bacterium has long been regarded as a testimony to the maturation of manure and manure. By the way, this time, the present inventor focused on the ability to solubilize lignin and fibrin-decomposing ability of the bacterium, and symbiotic mixed culture of the bacterium and the fibrinolytic bacterium significantly enhanced these two functions. , And also has a positive effect on other action functions. The success of this symbiotic mixed culture method has enabled the decomposition and utilization of various kinds of persistent organic materials, improved their productivity, and established a new method for producing a liquid fermenting soil activator. In this way, the new bacterium SK053
The formation of a healthy active soil microbial flora, which is one of the main bacteria, stabilizes the growth and function of soil-effective bacteria against changes in environmental factors, and diminishes soil diseases and continuous crop damage.

Mixed culture of new bacterium 053 and fibrinolytic bacterium 1. New culture SK053 large-scale culture Using MY medium, under aerobic conditions such as aeration or shaking, 6
Culture at 5 to 70 ° C. for 24 hours to obtain a culture containing cells. 2. Large-scale culture of fibrinolytic bacteria Using Omeliansky (1904) or Viljoen et al. (1926) medium (Viljoen et al., 1926) under anaerobic or aerobic conditions for 24 or 72 hours at each suitable temperature. And make the culture.

3. Mixed culture of new bacterium SK053 and fibrinolytic bacterium Using the following medium, inoculate an appropriate amount of a large culture of new bacterium SK053 and fibrinolytic bacterium, and inoculate each fibrinolytic bacterium up to 20 to 70 ° C. Cultivate at a suitable temperature for 24-72 hours to obtain a mixed culture of both bacterial species. Then, this is made into a granular material by using the excipient shown by the present invention. Immediately before application, an equal amount of a large amount culture of both bacterial strains is taken and mixed well before application.
The following E-medium for mixed culture is simply referred to as E-medium. E-medium for symbiotic mixed culture K2HPO4 5kg (NH4) 2SO4 2kg Urea 2kg Peptone 5kg Yeast extract 5kg Filter paper (fibrin) 15kg Micronutrients 200ml CaCO3 excess water (~ pH = 7.0) 1000l

4. Usefulness of symbiotic mixed culture of both strains Under conditions of high temperature environment, symbiotic mixed culture of SK053 strain of absolutely aerobic Thermoactinomyces sp. And anaerobic thermophilic fibrinolytic SK522 strain is SK522
It remarkably enhances the decomposition and fermentation of natural refractory organic materials, which is almost impossible with the strain alone. First, the growth of the SK053 strain supplements the proteolytic activity lacking by the SK522 strain. In addition, the decomposition and fermentation of organic materials is initially carried out in a well-ventilated environment. Then, the growth of the SK053 strain in the early stage is changed to a high temperature anaerobic environment, which enables the SK522 strain to grow in the natural environment. The growth of the SK053 strain continues until the final stage of maturity.

(1) Solubilization of lignin Here, it is particularly noted that SK053 bacterium exerts lignin solubilizing ability of SK522 strain mutually remarkably. The persistent organic material actually exists in a state in which the fibrin is strongly adsorbed or bonded to lignin and other organic components. Such a natural lignocellulosic substance can hardly be decomposed by single application of the SK522 strain having a strong fibrinolytic activity. The chemical structure of lignin is
Although it has not been completely clarified yet, phenylpropane, which has a side chain with three carbon atoms in the benzene ring, becomes the basic unit, and this unit is randomly and three-dimensionally generated by the radical reaction catalyzed by peroxidase. It is a polymerized polymer compound. It is a consensus view of many researchers and engineers that resistance to microbial decomposition is extremely strong and extremely difficult to decompose.

However, SK053 used in the present invention
The bacterium and the SK522 strain, although weak or weak, solubilize lignin. And this lignin solubilization ability is SK
By co-cultivating with the 053 bacterium in a symbiotic manner, they are mutually emphasized, for example, 50-60% of rice straw lignin.
It was revealed for the first time that the above was solubilized in a short time around 5 to 7 days. Its practical application is an important characteristic aspect of the present invention, and its action and effect are 50
It is exerted at a high temperature stage of -80 ° C or higher, and its humus ripening proceeds rapidly.

(2) Production of yellow pigment The yellow pigment produced by the SK522 strain is a carotenoid insoluble pigment. This yellow pigment in the cells is decomposed by other microorganisms and becomes a water-soluble low molecular weight substance,
It is absorbed by the plant and transferred to the required site, making it a convenient precursor. That is, such a yellow pigment not only promotes the growth and proliferation of rhizomes along with other lysed cell contents and secretions, and fermentation products, but also causes the formation of flower buds, fruit set, fruit enlargement, etc. It was clarified by research conducted at the molecular biological level that it was deeply involved in the metabolic system of reproductive growth, and it was developed into its application and utilization,
It has already helped to improve the quality of fruit such as taste, color and storability.

(3) Mixing and complexing of microbial ecosystem When the bacterial cells of the SK522 strain containing this yellow pigment are applied, the growth of general heterotrophic microorganisms, soil effective bacteria, etc. that propagate using it as a substrate is promoted. More importantly,
Aside from the SK053 bacteria, in the natural field, SK52
Thermophilic microorganisms such as the two strains are not immediately growth activated. Initially, general room temperature heterotrophic microorganisms grow and the fermentation heat due to these actions is accumulated to help increase the product temperature, and only a single strain of thermophilic fibrinolytic bacteria and a small number of microorganisms are available. , It is not involved in the humification of organic materials. It is required to mix and diversify the microbial flora. It is desirable to have a complex content that there are many effective bacteria of various types and that "feed (substrate)" is also appropriate. Thus, the high temperature decomposition of organic substances is
Bacteria flora mainly consisting of bacteria and SK522 strains are formed first, and the fermentation decomposition process is not only a simple change in the constituent components, but also an extremely complicated interaction and transition of the microbial flora with a deep relationship and is the most efficient. It progresses. SK053 strain, SK522 strain and SK542
Effective bacteria other than strains include the following.

(1) Culture of Hemicellulose Degrading Bacteria Hemicellulose forms a plant (cell wall) together with fibrin, and is called xylan, araban, dextran, mannan, galactan, etc., depending on the sugars constituting the plant. As a culture of hemicellulose-decomposing bacteria, Iwata's medium (1936) added to a concentration of rice xylan about 1% was used, and the cells were facultatively and anaerobically collected at 30 to 38 ° C. Usually Bacterium vulgatus, Bac. Prodigiosum, Bacteria mesentericu
s ruber), Microspyra Agariquefisense (M
A mixed culture of one or more strains such as icrospira agerliquefaciens) is obtained. (2) Cultures of pectin substance-degrading bacteria Many of the bacteria that strongly decompose pectin substances belong to the Bacillus subtilis group bacteria and ethanol / acetone bacteria when they are aerobic, and to the lactic acid bacteria when they are anaerobic. In the present invention, a Morish medium (Molisch 1939) is used, and soil, compost, horse manure, and spoilage such as bagasse and chomp are used as a separation source.
The inoculum is obtained at 27 to 35 ° C., 3 to 5 days of culture, and several rounds of culture in thick and thin layers. In the present invention, the culture of one or more strains is used.

(3) Soil actinomycete culture It is generally difficult to say about the action of actinomycetes (Actinomycetales Buchanan, 1917) in soil. But,
It decomposes various organic substances, especially incombustible fibrin and lignin, together with other microorganisms, and plays an important role in the formation of humus, which is the source of soil fertility. Certainly, it has important significance in terms of microflora control. For the culturing of actinomycetes, powerful bacteria are collected by using Waxman's medium (Waksman 1919), fertile soil as a separation source, and manure / manure. (4) Cultures of soil filamentous fungi and yeasts The most abundant locations of soil filamentous fungi are soils like bacteria and actinomycetes, and filamentous fungi in soils are naturally abundant in cultivated soil with plant roots, especially rhizosphere. Then, the work is also active. It is involved in the decomposition of organic substances such as plant remains and is related to soil fertility. It is considered that filamentous fungi are mainly active in the initial stage of decomposition. Next, there are many unclear points about the function of soil yeast. However, there are quite a few yeasts in the soil,
In addition, it is certain that the abundant vitamins and growth factors contained therein will affect coexistence with other microorganisms and soil activity. Cultures of soil filamentous fungi and yeast are separated and cultured from soil or manure / manure using a Tuapeck Dox medium (Czapek & Dox1910).

(5) Culture of thermophilic Bacillus genus Bacillus, which is generally aerobic, motile and has endospores, is a group of bacteria widely distributed in the natural world such as soil, leaf surface and hay,
It is also reported that most of the bacterial strains have strong heat resistance and can grow even at 55 ° C. or higher, and thus are major microorganisms during compost production. In addition, secreting antibacterial substances such as bacitracin and basilicin has attracted attention in the field of soil plant pathology in recent years. From these reasons, it is possible to try to utilize this bacterial group for crop production, and fertilized soil or a suspension of manure / manure is heat treated at 80 ° C. for 10 minutes to be used as a separation source. Waxman's medium (Waksman, 19
22), and aerobically collect the bacterial group at 50 to 60 ° C. In the present invention, single or mixed culture of these bacteria is used.

(6) Culture of yellow pigment-producing bacterium The yellow pigment produced by bacteria is a carotenoid pigment. There are many types of ripe compost, and many types. It is also often found on the leaves of plants. Using these as a separation source, a normal meat extract medium or peptone-yeast extract medium,
Incubate and separate at 25-35 ° C under aerobic conditions. It is easily identified by colors such as yellow-green, yellow, yellow-brown, and red, and is generally flavobacterium (Flavobacterium), chromobacterium (Chromobacterium), pseudomonas (Pseudomonas), serratia (Serratia), phototrophic bacteria (Phototrophic bacteria) A culture of one or more strains belonging to, etc. is obtained.

Next, the SK522 strain mixed with the SK053 strain of the present invention and the SK542 strain having a favorable effect on the growth and fermentation of the mixed culture will be described. [I] SK522 strain Clostridium thermocellum SK522 (Clostridium thermocellum biovar SK522, Micro Engineering Kenjoyori No. 3459, FERM BP-3459)
(Abbreviation SK522 strain) I. Scientific properties Bacteriological properties Cannot grow alone without companion bacteria. However, since the associated bacteria are easily isolated and pure-cultured, it is the result of testing various properties of the isolated associated bacteria while co-culturing them with the isolated associated bacteria as a basis. Shape There are also straight rod shape and slightly curved shape. Alone, sometimes two. 0.3-0.5 × 2.2-4.0 μ. When the culture becomes old, it extends into filaments, long-chain, and lengths of 5.7 to 12.8.
μ. Motility is not observed in pericardium and room temperature hanging drop specimens. Oval or circular spores are formed at the ends to expand the cells and form a club. Gram negative.

Culture properties (1) Plate culture It does not grow on plate culture using broth agar or other commonly used medium. However, while the medium presented by Viljoen et al., Tetrault circular filter paper agar plate forms colonies with associated bacteria and yellow spots, it cannot be produced by this bacterium alone. (2) Slope culture and stab culture Addition of Schweizer reagent-treated filter paper to Viljoe et al. Medium Agar slope culture and stab culture do not grow. (3) It does not grow on potato culture, gelatin culture surface, or puncture. (4) Liquid culture medium of Viljoen et al., Fibrin gravy,
Growth is observed only in a medium containing fibrin such as fibrin peptone water (poor growth). If a substance other than fibrin such as glucose or xylose is used as a carbon source, the decomposing power of fibrin is lost.

Physiological and biochemical properties 1. Enzymatic action, etc. (1) Fibrinolysis The fibrinolytic enzyme possessed by this bacterium is essentially composed of some β
It is a complex of -1,4-glucanase. Secreted extracellularly, action temperature 80 ℃ or more, same hydrogen ion concentration pH = 1
It was confirmed that several kinds of enzymes having heat resistance and alkali resistance of 0.0 or more were contained. It cleaves from the ends of macromolecules of fibrin that form a micellar structure to produce glucose, cellobiose, cellooligosaccharides and the like. When the substrates that act on these enzymes are mainly shown, it decomposes filter paper FP-ase: Positive Avicel is decomposed Avicelase: Positive Cellobiose is decomposed into two molecules of glucose Cellobiase: Positive (2) Lignin-decomposed Inawala lignin solubilization : Positive (weak, weak) (3) Proteolytic enzymes: Negative peptidase: Positive (4) Starch hydrolysis test: Slightly positive (5) Hemicellulose, xylan, pectin hydrolysis test : Negative (6) Invertase, Maltase: Positive (7) Lipolysis test: Negative (8) Oxidation reaction Hydroquinone reaction: Positive Tyrosine reaction: Negative (9) Reducing action: Positive

2. Product Test (1) Fibrin Fermentation Fermentation rate 78-91%, vigorous fermentation of fibrin, ethanol, methanol, acetaldehyde, acetic acid, lactic acid, formic acid, lactic acid, succinic acid, fumaric acid, tartaric acid, gluconic acid, It produces glucose, cellobiose, cellooligosaccharides, cellodextrin, large amounts of carbon dioxide, hydrogen, and hydrogen sulfide. (2) Produced acid from other sugars and alcohol Produced acid from glucose, sucrose, maltose, and cellobiose. (3) Gas generation test Although gas is generated more violently than fibrin, no gas generation is observed from glucose, sucrose, maltose, and cellobiose. (4) Peptone water test Ammonia: Slight reaction Indole: Positive skatole: Positive Hydrogen sulfide: Positive (5) Pigment production Usually, carotenoid yellow pigment production 3. Growth conditions (1) Growth temperature Optimum temperature is 65-72 ° C, temperature range is 40-80 ° C, 4
It does not grow below 0 ° C. (2) Hydrogen ion concentration Optimum hydrogen ion concentration pH = 6.7-8.0, the range is 5.6-9.6. (3) Nitrogen source Peptone is the best, and urea, uric acid, asparagine, sodium glutamate, etc. are also good. Ammonium salts are also good sources of inorganic nitrogen. (4) Carbon source When the subculture is continued with carbohydrates other than fibrin, its growth and fermenting power are lost. (5) Relationship with oxygen Eh = 200 to −250 mV, which is an anaerobic bacterium. (6) Requirement of micronutrients Micronutrients such as biotin, pyridoxamine, vitamin B12 and p-aminobenzoic acid are required. 4. Content of G + C of DNA mol% of G + C = 38-40 (Tm) is estimated.

II. Taxonomic position This SK522 fermenting fibrin vigorously at the optimum growth temperature of 65 to 72 ° C and temperature range of 40 to 80 ° C.
The following thermophilic fibrinolytic bacteria are similar to the strains. Clostridium ther
mocellum Viljoen, Fred and Peterson, 1926 .: Jour. A
gr. Sci. (London), 16,7 (1926).) C. dissolvens
Bergey et al. 1925, aka Bacillus cellulose dessolve
ns Khouvine, 1923 .: Ann. Inst. Past.37,711 (1923); Be
rgey's Manual, 2nd.Ed. (1925) p.344.) C. thermocellulas
eum Enebo, 1951.: Bergey's Manual, 7th.Ed. (1957) p.68
9.) Bacillus thermocellolitex
ellulolyticus Coolhaas, 1928.: Cent Bakt.II, 75,101
(1928), 76,38 (1929).) Bacillus thermofibrinco
lus Itano and Arakawa, 1929: Farming, 5,816,921 (1929); 6,
248,257 (1930).) Among these, Clostridium disorbens is a bacterium that is very similar to this strain in morphological, culture, and physiological biochemical tests except for the following four differences. (1) It has a lignin-solubilizing ability although it is weak or weak. (2) Suitable growth temperature of 65 to 72 ° C, growth temperature range of 40 to 80
Does not grow at temperatures below 40 ° C. (3) Actively ferment fibrin, but not hemicellulose, xylan, pectin, etc. (4) Lignin solubilization ability is the genus Thermus
It is markedly symbiotically emphasized by certain bacteria of the same type, and at the same time, has a favorable effect on the fibrinolytic activity and other action functions of this bacterium. However, at present, Berges' Manual (Bergsy's Manual
of Systematic Bacteriology Vol.2 (1986) p.1104, p.11
In 41.), only Clostridium thermocellum is described as a thermophilic degrading bacterium. All of them are treated as subspecies or variants of Clostridium dissolvens, or incompletely studied. Therefore, for the classification and identification of this strain, it was decided to compare and examine Clostridium thermocellum and various mycological properties thereof, and at the same time, other thermophilic fibrinolytic bacteria were also compared for reference.

In summary, the results of comparative examination of various mycological properties as described above show the characteristic properties of this strain and known thermophilic fibrinolytic bacteria including Clostridium thermocellum. The differences are the above-mentioned four items, and they can be enumerated in various ways such as the action on various other carbohydrates, the requirement of micronutrients, etc., but what I would like to emphasize especially is the problem with lignin. This strain has a lignin solubilizing ability, and the solubilizing ability is thermus aquaticus SK542 (Thermus aquaticus bi
It is significantly enhanced by the symbiotic mixed culture with ovar SK542). In contrast, there is no description about lignin of other thermophilic fibrinolytic bacteria including Clostridium thermocellum. If so, it is about its inhibitory effect on fibrin degradation. For this reason, a new strain (Strain) was established based on the physiological and biochemical properties of the lignin-solubilizing ability of this strain as one basis, and Clostridium thermocellum SK522 (Clostridium thermocellum biova
r SK522). This strain as a standard strain,
Clostridium ther
mocellum Viljoen, Fred and Peterson, 1926.) has the ability to solubilize lignin and is of the genus Thermus (genu
s Thermus Brock and Freeze 1969), which is characterized by significantly enhancing the lignin solubilizing ability by symbiotic mixed culture with a certain bacterium belonging to the SK522 strain and natural and artificial mutants It is a new strain depending on the physical properties.

Microbial Accession Number The microorganism accession number of this strain is Microtechnology Research Institute No. 3459 (FERMBP-3459).

Screening of SK522 strain Soil, beach sludge, manure / manure, human / livestock feces were isolated sources, and after repeated concentrated culture several times at 55-65 ° C., presented by Viljoen et al. Medium Tetrault circular filter paper Agar plates are used for isolation in a conventional manner. Viljoen et al. As medium
The salt composition presented by is suitable, but if necessary, a trace amount of inorganic metal salts, vitamins, growth promoting factors such as yeast extract and the like may be added. Viljoen, Fred and Peterson 1926 Peptone 5.0 g Calcium carbonate Excess ammonium phosphate sodium 2.0 g Potassium acid phosphate 1.0 g Magnesium sulfate 0.3 g Calcium chloride 0.1 g Ferric chloride Trace fiber Element (filter paper) 15.0 g Imizu 1000 ml

[II] SK542 Strain Thermus aquaticus biovar SK542 (Thermus aquaticus biovar SK542, Micro Engineering Kenjoyori No. 3382, FERM BP-3382) (abbreviation SK)
542 strain) I. Scientific properties Mycological properties Form Long rod-shaped, 0.4-0.6 × 3.0-5.0μ. Under certain conditions, for example, when culture becomes old, filamentous, length 20 to 130
μ. No flagella and no motility in room temperature hanging drops.
No endospores. Gram negative.

Culture property Proliferation is active. Generation time is 20 to 50 minutes. (1) Plate culture 3% agar, 60 ° C culture: yellow, relatively dense, small round colonies. (2) Agar stab culture Only surface growth, yellow slightly expanded. (3) Liquid culture Grows in a film on the surface (static culture).

Physiological and biochemical properties 1. Enzyme action, etc. (1) Proteolytic proteolytic enzyme: Positive (strong) (proteolytic enzymes) Gelatin hydrolysis: Positive peptidase: Positive (2) Starch hydrolysis: Slightly positive (3) Pectin degradation: Positive (weak) ) (4) Degradation of fibrin: Negative (5) Degradation of lignin: Negative Degradation of both fibrin and lignin is negative, but lignin digestion of SK522 strain is possible by symbiotic mixed culture with thermophilic fibrinolytic SK522 strain. It significantly enhances the solubilizing ability and, at the same time, positively affects the fibrin decomposing power and other action functions. (6) Lipolysis: Negative (7) Invertase, maltase: Positive (8) Catalase, oxidase reaction: Positive (9) Hydrogen sulfide production: Positive (weak) (10) Sulfate reduction reaction: Negative 2. Product test (1) Raw acid and gas generation from sugar and alcohol No gas generation. Glucose, galactose, maltose, lactose,
Raw acid from glycerol. (2) Formation of indole and skatole: Negative (3) Pigment production Yellow pigment (carotenoid pigment, maximum absorbance 450n
m, others with small peaks at 430, 435, 470 nm). However, it is scarcely produced in a synthetic medium containing glucose and other sugars as a carbon source.

3. Growth conditions (1) Medium concentration Bacteria of the genus Thermus are generally sensitive to organic matter concentrations. It is said that the less nutrients and the lower the concentration, the better. However, this strain is different from these in that it grows well even at normal concentrations. It grows even with salt NaCl 5% or more. Optimal concentration 2.
0-3.0%. In general, Genus Thermus bacteria do not grow in the presence of more than 2% NaCl. It grows even with sodium glutamate of 3% or more. It grows on sucrose or maltose 5% or more. Its growth is not inhibited even in the presence of 2% peptone + 1% yeast extract. Generally the genus Thermu
For the bacteria of s), trypton and yeast extract concentrations of about 0.1% are suitable, and they do not grow when they exceed 1%. (2) Growth temperature Optimum temperature is 72-76 ℃, growth temperature range is 40-82 ℃,
It cannot grow below 40 ° C. (3) Hydrogen ion concentration Optimal hydrogen ion concentration pH = 6.0 to 10.0. Growth hydrogen ion concentration range pH = 4.0 to 11.0. (4) Nitrogen source Proteins such as gelatin, glutamate, urea, and ammonium salt are often used as an inorganic nitrogen source. (5) Carbon source Glucose, sucrose, maltose, galactose, cellobiose, raffinose, stachyose, starch, glycerol, acetic acid, lactic acid, malic acid, glutamate are used. (6) Relationship with oxygen Absolutely aerobic. (7) Demand for micronutrients This strain has high demands for micronutrients. In addition to vitamins such as biotin, nicotinamide, and thiamine, a relatively high concentration of metal ions such as iron, manganese, and calcium is required for good growth of this strain. It is also sensitive to the amount of these minerals. (8) Susceptibility to antibiotics Like general Thermus genus (genus Thermus) bacteria,
It shows high sensitivity to penicillin G, chloramphenicol, tetracycline, streptomycin, kanamycin and other antibiotics. 4. Content of G + C of DNA mol% of G + C = 68 (Tm) is estimated.

II. Taxonomic position The bacterium does not grow in absolute aerobic condition, optimum growth temperature of 72-76 ° C, growth temperature range of 40-82 ° C, 40 ° C or lower. Since it is a non-spore-free, Gram-negative long bacillus, etc., in consideration of other mycological properties, the genus Thermus (genus Thermus Broc
k and Freege 1967). And, as a similar bacterium of the genus Thermus, Thermus aquaticus Broc
k and Freege 1969.:Bergey's Manual (1984) Vol.1, P.3
37.) Thermus thermophilus Osh
ima and Imahori 1974.:Int.J.Bacteriol.,24,102(197
4)) Thermus Flavus Saiki, Kimura an
d Arima 1972.:Agr.Biol.Chem.,36,2357(1972)), etc., but currently Bersey's Manual
s Manual of Systematic Bacteriology Vol.1 (1984) P.33
3) Genus Thermus Brock and Freeg 196
Bacterial species belonging to 7) are Thermus aquatics (The
rmus aquaticusBrock and Freege 1969),
Moreover, since it has characteristic properties that cannot be found in the known bacterial species as described below, this strain is used as a new strain (strain) of Thermus aquaticus SK542 (Thermus aquaticus biovar SK5
We conclude that it is reasonable to name it 42).

(1) The lignin-solubilizing ability is remarkably enhanced by symbiotic mixed culture with the thermophilic fibrinolytic bacterium SK522 strain. At the same time, it has a positive effect on the ability to decompose fibrin and other functions. (2) It has a strong proteolytic activity with a wide range of working hydrogen ion concentration and working temperature. (3) The genus Thermus (genus
(Thermus) bacteria are very weak and grow well in medium of normal concentration. (4) Strong demand for micronutrients, demand for various vitamins and metal ions, and sensitivity to their mineral contents. (5) Yellow pigment (carotenoid pigment, maximum absorbance 45)
0 nm and others have small peaks at 430, 435 and 470 nm).

The SK542 strain is a standard strain of this strain, and the Thermus aquaticu (Thermus aquaticu)
s Brock and Freege 1969), by symbiotic mixed culture with a thermophilic fibrinolytic bacterium (SK522 strain), its lignin solubilizing ability was remarkably enhanced and at the same time, the fibrinolytic activity was also affected. And a new strain (strain) having physiological and biochemical properties that includes the SK542 strain and natural and artificial mutant strains. Microbial Accession Number The microorganism accession number of this strain is Microtechnology Research Institute Article No. 3382 (FERMBP-3382).

Screening of SK542 strain Isolate sources are hot spring sources in and around Kyushu, soils near hot spring sources, humus, and the like. The separated sample is precultured at 55 to 60 ° C. and then isolated by 3% agar plating according to a conventional method. Separation medium composition (Castenholz medium: Castenholz, RW; Bacteriol.
Rev., 33, 467 (1969)) Nitrilotriacetic acid 100 mg CaSO4 · 2H2O 60 mg MgSO4 · 7H2O 100 mg NaCl 8 mg KNO3 103 mg NaNO3 689 mg Na2HPO4 111 mg FeCl3 0.28 mg MnSO4 · H2O2 H2O3H0O 0.016 mg Na2M0O4 · 2H2O 0.025 mg (yeast extract 5000 mg) (tryptone 5000 mg) (sucrose 10000 mg) Total amount (pure water, pH = ~ 8) 1000 ml Note: The composition in () is modified by the inventors.

Application Example It is advantageous and appropriate to treat the organic material as a raw material by dividing it into two, animal and vegetable.
Exposed manure that ripens animal seafood, domestic animals, leather, animal horns, etc., and maze grass, wild grass clippings, russin,
Green grass such as Tsurumurasaki, grass fermented fruit skin, etc.
Explain the two Mizugoe of fruit manure.

Application Example 1. Exposed fertilizer Ingredients: Cooked seafood residue in kitchen Kitchen container: 100l plastic container Inoculum: New culture SK053 mass culture (see 0027)
A culture of a pre-synthesized bacterium (see 0037) is added to the above to give a seed culture. Fermentation: 30 kg of raw materials, 30 kg of water, 50 ml of inoculum Fermentation temperature 20 to 30 ° C, initial 2 to 3 times a day, 2 weeks later 2
Stir once every 3 days, complete fermentation in about 1 year Product: Take supernatant and use as exposed fertilizer

Application Example 2. Vegetable juice fertilizer Ingredients: Shredded pomegranates, vines, rusan, etc. Vessels: 100 l Polytank Inoculum: New bacteria SK053 and fibrinolytic bacteria (see 0028) Fermentation: 20 kg of raw materials, 60 kg of water , Inoculum 50 ml Fermentation temperature 20-30 ° C, initial twice a day, after 3 weeks 2-3
Stir once a day. Fermentation is completed in about 2 months. Product: Take the supernatant to make blue juice

Application Effect To see the application effect of the above liquid fermenting soil activator, a field cultivation test of tomato was conducted. Test liquid fermented soil activator: Equivalent mixture of the above-mentioned blur manure and blue / juice fertilizer (see 0028) Field: Black soil, volcanic ash soil Species: Momotaro T93, Takii Seed Co., Ltd. Fertilization: per 1m, granulated formation Fertilizer (4: 8: 4) 30
0 g of liquid fermenting soil activator application zone, 500 ml of 500 times diluted solution was put into the planting hole at the time of fixed planting, and 200 ml of the same diluted solution was mixed twice in one crop. Planted: application zone and control zone, 12 m each,
Among them, 30 strains, the planting cultivation test results on March 20, are as shown in Tables 2 and 3. Table 2 Yield table by tomato quality (average per strain) ────────────────────────────────── Upper fruit Small fruit Random fruit average kg kg kg kg ────────────────────────────────── Control section 140.2 14. 6 2.5 157.3 Application area 205.8 0.9 0.4 207.1 ────────────────────────────── ───── Small fruits are regular fruits weighing less than 11g, and the total yield is until August 31st. Table 3 Soil microbial flora ───────────────────────────────── Microorganism application zone Control zone ───── ──────────────────────────── Thermophilic bacterium (including facultative anaerobic bacterium) × 10 ⁶ / g 15.6 1.7 Actinomycetes × 10 ⁶ / g 25.8 0.3 Filamentous bacteria × 10 ⁴ / g 4.6 1.4 Thermophilic fibrinolytic bacteria × 10 ³ / g 5.7 − ──────── ────────────────────────── M. P. According to the N method. In the liquid fermented soil activator application section, from the bottom to the top, the bells set and enlarged, and the soil and fruit finally reached a little less than 1.5 times.
The quality was tripled and the yield was high. In addition, there was no noticeable damage from diseases or insects without disinfection. In the soil microbial flora, the number of cells in the applied area is higher than that in the control area,
It is noted that there are many actinomycetes and thermophilic fibrinolytic bacteria.

[0058]

INDUSTRIAL APPLICABILITY As described above, the present invention provides a useful new bacterium, SK.
053 is provided. In addition, new strain SK053 and new strain SK
The success of symbiotic co-cultivation with fibrinolytic bacteria such as 522 has made possible the practical use of several action systems of microbial flora in natural ecosystems, agricultural ecosystems, and plant factory production systems. Was assured. One of them is a liquid fermenting soil activator. Appropriate application of the liquid fermentation activator of the present application is characterized by improving the quality of cultivated plants and high yield, but further application of the liquid fermentation soil activator as in the present invention is (1) ) The soil permeability is extremely improved. Replenishing soil and water is an important technology in greenhouses, greenhouses and plant factories, but water with good permeability and high nutrient content is effective for rooting and soil making. (2) In addition, the hardened soil also becomes aggregated and becomes a soft soil without realizing it. (3) Soil hydrogen ion concentration is neutral, weakly acidic or weakly alkaline, and at the same time electrical conductivity is corrected. (4) In addition, soil pest damage is reduced, physiological disorders are prevented, and the residual stem roots after harvesting are decomposed more quickly, resulting in less harmful effects of introducing immature organic matter. However, such effects are unthinkable with conventional fertilizer-based fertilizer and fertilizer application techniques. Not only the slow effect of organic fertilizers. Of course, it's also different from compost. What is the effect of the excellent liquid fermenting soil activator of the invention of the present application, and what is necessary is to change the concept. That is, to give citizenship to soil microorganisms that have been ignored in the past. One of the important factors in conventional fertilization techniques and cultivation theory is to take soil microorganisms into consideration. The application effect of the liquid fermenting soil activator of the present invention is that root-microorganism-soil, a relationship between the three, by applying agglomerates of effective microorganisms centering on thermophilic actinomycetes around the roots of cultivated plants. It can be concluded that it was obtained because the soil was activated and the soil became particularly healthy, especially in the rhizosphere.

─────────────────────────────────────────────────── ─── Continuation of the front page (51) Int.Cl. ⁶ Identification code Internal reference number FI technical display location C12R 1: 145) C09K 101: 00

Claims (1)

[Claims] 1. A novel thermophilic actinomycete, Thermoactinomyces SK053 sp. Nov. (Thermoactinomyces) according to 1 below.
SK053 sp.nov., Institute of Biotechnology, Industrial Technology Institute
Accession number FERM P-13598) and the new thermophilic fibrinolytic bacterium Clostridium thermocellum SK522 (Clostridium thermocellum biovar.SK) described in 2 below.
522, a liquid fermenting soil activator containing as an active ingredient a symbiotic mixed culture with Microtechnology Research Center No. 3459). Note 1. Thermophilic actinic radiation belonging to the genus Thermoactinomyces, having a wide range of growth temperature of 10 to 85 ° C, a wide range of growth hydrogen ion concentration of 5.3 to 10.8 pH, and having lignin solubilizing ability and fibrin decomposing ability. Bacteria Thermoactinomyces SK053s
p. nov. (Thermoactinomyces SK053 sp. nov., Institute of Biotechnology, Institute of Biotechnology, Contract No. FERM P-1
3598) Note 2 It has a lignin solubilizing ability, and a suitable growth temperature is 65 to 72 ° C.
A thermophilic fibrinolytic bacterium, Clostridium thermocellum SK522 (Clostridium thermocellum biovar.
K522, Microtech Research Institute No. 3459