JPH08134448A - Solid fermentation soil activator - Google Patents

Abstract

PURPOSE: To obtain a solid fermentation soil activator which is useful for fertilizing soil, improving soil structure, and improving the growth and qualities of cultivated plants by using, as the main effective component, a symbiotic mixed culture product of a specific thermophilic actinomycete and another kind of fibrin-decomposing bacteria. CONSTITUTION: This solid fermentation soil activator contains, as the main effective component, a symbiotic mixed culture product comprising a thermophilic actinomycete, thermoactinomyces SKO53 sp. nov. (Agency of Industrial Science and Technology, Life Engineering Research Institute. FERM P-13598), which belongs to the genus thermoactinomyces and has a growth temp. range of 10-85 deg.C, a growth pH range of 5.3-10.8, and lignin-solubilizing and fibrin-decomposing capabilities and another kind of fibrin-decomposing bacteria. An esp. effective example of the latter bacteria is a kind of thermophilic fibrin-decomposing bacteria, Clostridium thermocellum biovar. SK522 (Fermentation Research Institute FERM BP-3459). Other examples of the latter bacteria include methanogens and hydrogenomonas in addition to aerobic, anaerobic, and thermophilic fibrin-decomposing bacteria.

Description

Detailed Description of the Invention

[0001]

[Industrial application] New strains useful for promoting fermentative ripening of plant organic substances to improve soil fertilization and soil structure, and at the same time improve the growth and quality of cultivated plants and their effective utilization. Regarding technology.

[0002]

2. Description of the Related Art The solid fermenting soil activators described in claim 3 of the present invention, such as blurring manure and soil, are all clever and interesting uses of organic substances in Japan since ancient times.
However, the raw material, the adjustment of water content and pH, the inoculum to be inoculated, the fermentation system is a batch system or continuous fermentation, the fermentation conditions are aerobic or anaerobic, and the fermentation temperature has various fermentation days. The "fermenting microorganisms themselves, their physiology, and mechanism", which is essential for both research and technology, are discussed as "black boxes". And regarding the appearance of the whole microorganisms in these ripening processes, especially the function of effective microorganisms, since the ecological handling of the microflora in which such solid substrates are locally accumulated has not been established,
The reality is that I don't know.

[0003]

Under these circumstances, the present applicants have been pursuing these fermentation and ripening processes for more than 10 years from the standpoints of plant physiology / biochemistry and rhizosphere microorganisms, especially microbial ecology. 1) Identification of microorganisms involved in fermentation and ripening. (2) Return of dominant microflora during ripening process and its ecological study. Main microorganisms and their effects. (3) Production of a solid fermenting soil activator which is a standard under the conditions in the test field. (4) Measurement of changes in the main ingredients of raw materials during the aging process. (5) Reasonable maturity index. (6) Relationship between the growth inhibition of raw raw materials and the prevention of pest damage and the identified microorganisms. At the same time as trying to elucidate the above problems, the results of the test research are the screening of some effective bacteria, the elucidation of the theory of substance decomposition by symbiotic culture, and the new solid fermented soil mainly composed of thermophilic actinomycetes. Activator provided.

[0004]

Means for Solving the Problems The constitution of the present invention which has solved the above problems is as follows: 1) belongs to the genus Thermoactinomyces and has a wide range of growth temperature of 10 to 85 ° C. and a wide range of 5.3 to 10.8 pH. Thermophilic actinomyces SK, a thermophilic actinomycete that has a growing hydrogen ion concentration and has lignin solubilizing ability and fibrin degrading ability
053 sp. Nov. (Thermoactinomyces SK053 sp. Nov.,
Institute of Biotechnology, Institute of Biotechnology, Contract No. FERM
P-13598) and an individual fermenting soil activator whose main active ingredient is a symbiotic mixed culture of other fibrinolytic bacteria. 2) Thermophilic fibrinolytic decomposition of other fibrinolytic bacteria according to 1) above. Bacterial Clostridium thermocellum SK522 (Cl
ostriduim thermocellum biovar. SK522, Microtechnical Laboratory Article 3459)).

The other fibrinolytic bacteria according to the claims of the present invention are the thermophilic actinomycetes Thermoactinomyces SK of the present invention.
053 sp. Nov. (Thermoactinomyces SK053 sp. Nov.,
Institute of Biotechnology, Institute of Biotechnology, Contract No. FERM
P-13598) and a thermophilic fibrinolytic bacterium Clostridium thermocellum SK522 (Clostridiumthe
rmocellum biouar sk522 is a fibrin-degrading bacterium except for Microken Kojo No. 3459), which is aerobic, anaerobic, thermophilic,
Physiologically fermenting the fibrin, especially methane bacteria that generate a large amount of methane gas, or hydrogen bacteria that generate hydrogen are also included in this group. Specifically, there are the following.

(1) Aerobic fibrin-decomposing bacteria (suitable temperature 22-35)
℃) Bacillus fimi (Cellulomonus fumi), others genus Cel
lulomonus, Cellfalcicula viridis, C.mucosa Actinomyces melanocyclus (Micrococcus melanocyclu
s), other genus Actinomyces Cellulyticum flavum Celluvibrio flavescens, other genus Celluvibrio Cytophaga hutchinsonii, other genus Cytophaga Pseudomonas fibrolysis Methanobacterium soehngenii, M.omelianskii Sarcina methanica

(2) Anaerobic fibrin-decomposing bacteria (suitable temperature 25-37
℃) Clostridium werneri Plectridium cellulolyticum

(3) Thermophilic fibrin-decomposing bacteria (suitable temperature 55-65
℃) Clostridium thermocellum, C.thermocellulaseum Bacillus cellulosae dissolvens, B.thermofibrincol
us

(4) Bacterium cellaresolvens, a denitrifying fibrin-degrading bacterium (35 ° C)

As used in the specification, the hardly decomposable organic material or the hardly decomposable fiber material means sawdust, chip dust, planer refuse, bark (bark) and other waste materials in the wood industry, straw rods such as rice husks, rice straws and wheat straws. , Soybean, azuki bean, fallen seeds and soybean seed coats and pods, coffee grounds, deciduous leaves, bark, mountain grasses such as reeds and kaya, as well as abolished mushrooms such as shiitake mushrooms and other mushrooms 100
The above-mentioned various persistent plant bodies. The organic components that make up these plant remains are extremely complex and diverse, but generally, the main component is fibrin, which is about 30-75.
%, And then 15 to 40% of lignin and 45 to 90% or more when both are combined, and occupy most of them. Next is hemicellulose in the order of 7 to 25%, and in a small amount, nitrogen-containing compounds such as proteins, various sugars, organic acids, alcohols, and fats, waxes, essential oils and the like are contained.

The hard protein referred to in the specification is water,
Proteins such as collagen, keratin, otscein, and fibroin that are insoluble in salt solutions, light acids, and light alkalis, are not easily hydrolyzed by acids and alkalis, and are not easily affected by enzymes, such as bones and teeth, horns, nails, and hair. It is the main component.

[0012] The excipients used in the present invention are limestone rock powder, dolomite rock powder, shell fossil powder, crab shell, shellfish shell powder such as shellfish, calcium carbonate, slaked lime, perlite,
There are powder particles such as vermiculite, zeolite, diatomaceous earth, basic rock rock powder, ground peat moss, charcoal and charcoal powder, and one kind or a mixture of plural kinds thereof is shown.

The micronutrients and trace minerals of the present invention include vitamins such as biotin, nicotinamide, thiamine, pyridoxamine, vitamin B12, balaaminobenzoic acid, arginine, cystine, glutamic acid, isoleucine, leucine, proline, lysine and methionine. , Phenylalanine, threonine, tryptophan, tyrosine, valine, and other amino acids, and iron, manganese, copper, cobalt, calcium, magnesium, and other trace minerals. As described above, these excipients, micronutrients and trace minerals are appropriately selected according to effective bacteria, purpose of use, soil environment, climate and climate. The thermophilic actinomycete of the present invention, Thermoactinomyces SK053 sp. Nov.
yces SK053 sp. nov., Institute of Biotechnology, Institute of Biotechnology, Accession No. FERM P-13598) is hereinafter simply referred to as a new strain SK053 or SK053 strain.

[0014]

The present invention provides a useful new bacterium, SK053.
The present invention also relates to a mixed culture of the SK053 bacterium and a fibrinolytic bacterium and its effective use. The synergistic effect of the new bacterium SK053 and various fibrin-degrading bacteria enhances the solubilizing ability of lignin, and enhances the degrading ability of fibrin and other functions.

In particular, the new strain thermophilic fibrinolytic bacterium Clostridium thermocellum SK522 (Clostrid
uim thermocellum biovar.SK522, Micro Engineering Lab. No.3
459) (hereinafter new strain SK522 or SK522)
(Abbreviated as strain) and thermophilic mixed culture (60-65 ° C)
Is due not to the additive effect of the functions of each new strain, but to the synergistic effect caused by the interaction, which mutually enhances the function and strongly and stably stabilizes various natural fiber substances which are considered to be the most difficult. Fermenting and digesting lignin, fibrin, proteins, hemicellulose and other organic components quickly and strongly.

By using the mixed culture of the present invention as an active ingredient, solid fermenting soil activators of various organic materials can be obtained. The solid fermenting soil activator of these organic materials,
Depending on the use, purpose, climate, climate, soil composition, etc., the new strain SK053, in addition to the selected fibrin-degrading bacteria, can be mixed with useful microorganisms to further enhance the effect.

[0017]

EXAMPLES The present invention and examples of use thereof will be described in detail below. The scientific properties of the novel bacterium SK053 of the present invention are as follows.

Thermoactinomyces SK053 sp. Nov. I. Thermoactinomyces SK053 sp. Scientific properties Morphological characteristics Heat-resistant true endospore formation, forming single spores in basal hyphae and aerial hyphae. Spores are smooth, spherical, diameter 0.4 to 0.7
μ. Lacking or very short spore stalk. Basal hyphae are non-dissociative and have a diameter of 0.5μ. Very vigorous growth on agar medium, promptly forming aerial hyphae, white and powdery. Gram positive. Cultural properties Growth is extremely active. Colonies are formed in 4 to 6 hours and covered with white aerial hyphae. (1) Plate culture In addition to nutrition, yeast / malt, oat mill, sucrose / nitrate, glucose / asparagine, glycerin /
60 ° C culture of synthetic agar medium (3%) such as asparagine and tyrosine is very good and forms aerial hyphae. The colony surface has a white flora. The surface and back of the mycelium are colorless. There is no production of diffusible dye in the medium. (2) Liquid culture Meat static culture: A white film is formed. Often forms compact precipitates. Milk culture: coagulation, peptonization. Physiological and biochemical properties This bacterium markedly enhances solubilization of lignin by symbiotic mixed culture with various thermophilic, mesophilic, psychrophilic or anaerobic, aerobic or denitrifying fibrinolytic bacteria. At the same time, it also has a positive effect on the fibrin resolution. The water-soluble low-molecular-weight or decomposed substance produced in this way is directly absorbed and utilized by the plant or promotes the growth of soil-effective bacteria to establish a healthy and vigorous plant rhizosphere microbial ecosystem. And
As one of the pathways of carbon cycle in agricultural systems and natural ecosystems, the decomposition and humification of hard-to-decompose natural fiber materials is carried out strongly, stably and surely. 1. Enzyme action, etc. (1) Proteolytic enzyme (Proteolytic enzyme): Positive (strong) Gelatin hydrolysis: Positive Peptidase: Positive (2) Starch hydrolysis: Positive (3) Pectin degradation: Negative (4) Degradation of fibrin: positive (weak) (5) Degradation of lignin: positive Degradation of fibrin and lignin is both positive but weak. However, symbiotic mixed culture with fibrinolytic bacteria strongly solubilizes lignin and at the same time enhances fibrin degradability. (6) Lipolysis: Negative (7) Invertase: Negative (8) Catalase reaction: Positive (9) Okinidase reaction: Positive 2. Product test (1) Raw acid and gas generation from sugar and alcohol No gas is generated. (2) Pigment production: Formation of melanin-like pigment (tyrosine and peptone yeast iron agar): negative 3. Growth conditions (1) Growth temperature Optimum temperature 25 to 78 ℃, growth temperature range 10 to 85 ℃, the growth range is also very wide, and it has characteristics not found in other Thermoactinomyces species. (2) Hydrogen ion concentration Optimum hydrogen ion concentration pH = 6.5 to 9.6. Growing hydrogen ion concentration range pH = 5.3 to 10.8. This is also one of the characteristics not found in other species of the same genus. (3) Assimilation of nitrogen sources Organic and inorganic nitrogen sources are often used. (4) Assimilation of carbon source (on Pridham-Godlieve agar medium) D-glucose, sucrose, cellobiose, maltose, starch, organic acid, and glutamate are used. (5) Relationship with oxygen Absolutely aerobic. (6) Requirement of micronutrients In addition to biotin, thiamine, nicotinic acid, etc., flavonoid pigments such as rutin and hespyridine (Flav
Micronutrients such as onoid pigment are required. Furthermore, relatively high concentrations of ions such as phosphate and potassium are required for good growth of this bacterium, and the presence of carbon dioxide gas
Required for its growth and germination of spores. (7) Harmfulness to health or environment It is said that there are some pathogenic strains of bacteria belonging to this genus.
As far as this strain is concerned, I do not know that it has such properties. Separation sources Mainly high-speed high-temperature compost, aged muck / manure, agricultural soil, etc. Content of G + C of DNA mol% of G + C = 52.7 to 54.2 (Tn).

II. Taxonomic position The International Committee on Syste
Approval list of bacteriology names edited by matic Bacteriology (ICSB) (1980) and edited by Takeharu Hasegawa (Yoshiro Okami,
Seino Akio): Classification and identification of microorganisms (bottom), p. 1-92
(1985), Berger's Manual (Berg
ey's Manual of Determinative Bacteriology 7 ed., 1
957; 8ed., 1974; Bergey's Manual of Systematic
Bacteriology vol.1, vol.2, 1984, 1986.) was performed as a reference. As a result, Thermoactinomyces (genus
Thermoactinomyces)). The classification criteria in Burger's manual, which is generally used for the search and identification of bacteria and actinomycetes, cannot be said to be perfect, and there are many uncertainties. Especially for actinomycetes genus Thermoactinomyces (genus Thermoactinomyces) Even the categorical affiliation is ambiguous. The same manual (198
According to 6), it is very similar to the genus Bacillus, and this genus belongs to other actinomycetales.
) Is excluded because it has a low phytophilicity, but it is not included in the family of Bacillaceae.
In addition, the Genus Thermoacti
There are 3 species belonging to nomyces) in the Burger's manual (7th edition, 1957) and 3 in the same manual (8
Edition, 1974), 5 types, and the same systematic (2
Vol. 1986), Type species T. vulga
Only one species of ris is listed. Also, in other classification and identification documents, the number of species to which they belong varies, Nonomura, Ohara et al. (1) has 3 species, and ICSB has so far been the reference species Th.
ermoactinomyces vulgarisTsiklinsky 1898, Type stra
in: In addition to KCC A-0162, 5 types are approved names (nomen approb
atum). Actinomycetes,
The classification criteria of Order Actinomycetales are slightly different from those of other bacteria. For example, actinomycetes are characterized as a treasure trove of useful physiologically active substances such as antibiotics, and the classification of actinomycetes has made rapid progress in recent years in parallel with the remarkable recent development of antibiotics research. . That is, comparison of DNA base composition and sequence, DNA
In addition to genetic biochemical methods, which mainly consist of hybridization (DNA-hybridization) tests and other molecular-level analysis, serological immunological classification, classification by phage sensitivity, classification by constituent sugar and lipid composition of bacterial cells, whole bacteria From the idea that the classification by the IR spectrum of is attempted, and at the same time, the development of electronic computing equipment will simultaneously create a highly objective classification system.
Numerical taxonomy and other methods have been tried, and so-called new methods of chemical taxonomy (Chemotaxonomy) have been introduced rapidly. However, the introduction of these new findings into actinomycetes and bacterial taxonomy is in part, largely based on traditional classical taxonomy, based on characteristic phenotypic traits. It is not an exaggeration to say that we are in a very transient period. Therefore, at the present time, this actinomycete has been determined to be a new species, and Thermoactinomyces
SK053 sp. Nov. (Thermoactinomyces SK053 sp. Nov.)
Is displayed. Finally, a comparison of the main scientific properties that should be mentioned in the taxonomy between this actinomycete and the reference species of the genus Thermoactinomyces Thermoactinomyces brigaris is shown below (see Table 1). Document (1) Hideo Nonomura, Takeshi Ohara: Journal of Fermentation Engineering, 49, 8
95 (1971).

[Table 1] Description of Thermoactinomyces bulgaris (T. vulgaris), a reference species of the genus Thermoactinomyces in Table 1 (descriptio
n) is Tiskrinsky (Tsikl) about 100 years ago
insky). Therefore, there is no development of instrumental analysis techniques and microanalysis as at present, and there is no consideration for chemical taxonomy or consideration for production of physiologically active substances such as antibiotics. Under such an historical background, a comparative examination of the scientific properties of the present strain and the reference strain, including closely related strains, revealed the following characteristic differences, and the Thermoactinomyces genus (genus Thermoacti
nomyces) and a new strain.

(1) Heat-resistant genuine endospores are formed one by one on the basal hyphae and aerial hyphae. It has a high growth temperature and is highly thermophilic, and it can be definitely identified as the Thermoactinomyces genus, but it is very different from the reference species in that it has an optimum growth temperature of 25 to 78 ° C.
In the same temperature range of 10 to 85 ° C, it is much higher than the reference species and also extremely low. The temperature range for growth is generally thermophilic, mesophilic, and low temperature. (2) Optimum hydrogen ion concentration for growth pH = 6.5-9.
6, concentration range pH = 5.3 ~ 10.8 and acidic, neutral,
It has a wide range of alkalinity and concentration, and is strong on the alkaline side. (3) The enzyme activity of the reference species is positive for both gelatin solubilization and starch hydrolysis, but there is no description of negative fibrin degradation or lignin solubilization. Although there is a description that fibrinolysis is positive in closely related bacteria, it is very weak and cannot be used for industrial production. Further, there is no description about the enzyme activity for lignin. Therefore, the ability of this bacterium to decompose fibrin and the ability to solubilize lignin is the second characteristic of this bacterium. Then, by symbiotic mixed culture of this bacterium with various thermophilic, mesophilic, psychrophilic, or anaerobic, aerobic, or denitrifying fibrin-degrading bacteria, the solubilizing ability of lignin is significantly enhanced, At the same time, it has a positive effect on the fibrin resolution. This action of the present bacterium is a point that the present inventor particularly wants to emphasize, and the discovery of this specificity is an idea which is a key point of the present invention.
In addition, this bacterium has a strong proteolytic activity and decomposes hard proteins well.

(4) In addition to biotin, thiamine, nicotinic acid and the like, micronutrients such as flavonoid pigments such as rutin and hespyridine are required for vigorous growth of the bacterium. (5) The bacterium is absolutely aerobic, Gram-positive, positive for catalase reaction and oxidase reaction, and G + of DNA
Mol% of C = 52.7 to 54.2 (Tn),
It is less closely related to other actinomycetes and is a genus Bacillus (genus Ba
This is the reason why it has a strong affinity with cillus).

For this reason, the specificity of the growth temperature of the bacterium and the physiological and biochemical properties of the lignin-solubilizing ability of the bacterium were determined to be a new species as one basis, and Thermoactinomyces SK053sp.nov. (Thermoactinomyces SK0
53 sp.nov.).

Using this strain as a reference strain, among the strains belonging to the genus Thermoactinomyces,
It has a lignin solubilizing ability, and is characterized by significantly increasing the lignin solubilizing ability by symbiotic mixed culture with a fibrinolytic bacterium, and includes a physiology biochemical including SK053 strain and natural and artificial mutants. It is a new bacterial species depending on its properties.

Microorganism accession number The microorganism accession number of this bacterium is the accession number FERM P-13598 of the Institute of Biotechnology, Institute of Industrial Science and Technology.

Screening of New Bacterium SK053 Mainly, high temperature and high speed compost, matured manure / manure, agricultural soil, etc. are used as a separation source and agar plate culture is performed at 55-60 ° C. to perform separation according to a standard method. MY medium is used as the medium. It is advisable to add micronutrients as needed.

Utility of new bacterium SK053 The new bacterium SK053 originally appears as a white, powdery microorganism in high-quality compost, or fully matured compost and manure. The antibacterial property against harmful bacteria is also well known. The appearance of this bacterium has long been regarded as a testimony to the maturation of manure and manure. By the way, this time, the present inventor focused on the ability to solubilize lignin and fibrin-decomposing ability of the bacterium, and symbiotic mixed culture of the bacterium and the fibrinolytic bacterium significantly enhanced these two functions. It has also been found that it has a positive effect on the action function of other people. And the success of this symbiotic mixed culture method enables the decomposition and utilization of many types of persistent organic materials, improves their productivity, and secures the conventional open-field composting method, and high-temperature high-speed composting. Law (Hi
GH rate composting) has been established. In this way, the formation of a healthy active soil microbial flora with the new bacterium SK053 as one of the main bacteria stabilizes the growth and action function of the soil effective bacteria against changes in environmental factors, and prevents soil diseases and continuous crop damage. Diminished and extinguished. In addition, it is expected to develop a new technology for utilizing microorganisms in a new dimension, such as new technology of directly spraying on the soil or the leaf surface of plants and deodorizing decomposition of human waste, decomposition of hard proteins, and fermentation treatment of food waste.

Mixed culture of new bacterium 053 and fibrinolytic bacterium 1. New culture SK053 large-scale culture Using MY medium, under aerobic conditions such as aeration or shaking, 6
Culture at 5 to 70 ° C. for 24 hours to obtain a culture containing cells. 2. Large-scale culture of fibrinolytic bacteria Using Omeliansky (1904) or Viljoen et al. (1926) medium (Viljoen et al., 1926) under anaerobic or aerobic conditions for 24 or 72 hours at each suitable temperature. And make the culture.

3. Mixed culture of new bacterium SK053 and fibrinolytic bacterium Using the following medium, inoculate an appropriate amount of a large culture of new bacterium SK053 and fibrinolytic bacterium, and inoculate each fibrinolytic bacterium up to 20 to 70 ° C. Cultivate at a suitable temperature for 24-72 hours to obtain a mixed culture of both bacterial species. Then, this is made into a granular material by using the excipient shown by the present invention. Immediately before application, an equal amount of a large amount culture of both bacterial strains is taken and mixed well before application.
The following E-medium for mixed culture is simply referred to as E-medium. E-medium for symbiotic mixed culture K2HPO4 5kg (NH4) 2SO4 2kg Urea 2kg Peptone 5kg Yeast extract 5kg Filter paper (fibrin) 15kg Micronutrients 200ml CaCO3 excess water (~ pH = 7.0) 1000l

4. Usefulness of symbiotic mixed culture of both strains Under conditions of high temperature environment, symbiotic mixed culture of SK053 strain of absolutely aerobic Thermoactinomyces sp. And anaerobic thermophilic fibrinolytic SK522 strain is SK522
It remarkably enhances the decomposition and fermentation of natural refractory organic materials, which is almost impossible with the strain alone. First, the growth of the SK053 strain supplements the proteolytic activity lacking by the SK522 strain. In addition, the decomposition and fermentation of organic materials are all in the state of solid fermentation, and are in an environment with good ventilation. Then, the growth of the SK053 strain in the early stage is changed to a high temperature anaerobic environment, which enables the SK522 strain to grow in the natural environment. Then, the growth of the SK053 strain continues until the final stage of full maturity.

(1) Solubilization of lignin Here, it is particularly noted that SK053 bacterium exerts lignin solubilizing ability of SK522 strain mutually remarkably. The persistent organic material actually exists in a state in which the fibrin is strongly adsorbed or bonded to lignin and other organic components. Such a natural lignocellulosic substance can hardly be decomposed by single application of the SK522 strain having a strong fibrinolytic activity. The chemical structure of lignin is
Although it has not been completely clarified yet, phenylpropane, which has a side chain with three carbon atoms in the benzene ring, becomes the basic unit, and this unit is randomly and three-dimensionally generated by the radical reaction catalyzed by peroxidase. It is a polymerized polymer compound. It is a consensus view of many researchers and engineers that resistance to microbial decomposition is extremely strong and extremely difficult to decompose.

However, SK053 used in the present invention
The bacterium and the SK522 strain, although weak or weak, solubilize lignin. And this lignin solubilization ability is SK
By co-cultivating with the 053 bacterium in a symbiotic manner, they are mutually emphasized, for example, 50-60% of rice straw lignin.
It was revealed for the first time that the above was solubilized in a short time around 5 to 7 days. Its practical application is an important characteristic aspect of the present invention, and its action and effect are 5
It is exerted in the high temperature stage of 0 to 80 ° C. or higher, and its humus ripening progresses rapidly.

(2) Production of yellow pigment The yellow pigment produced by the SK522 strain is a carotenoid insoluble pigment. This yellow pigment in the cells is decomposed by other microorganisms and becomes a water-soluble low molecular weight substance,
It is absorbed by the plant and transferred to the required site, making it a convenient precursor. That is, such a yellow pigment not only promotes the growth and proliferation of rhizomes along with other lysed cell contents and secretions, and fermentation products, but also causes the formation of flower buds, fruit set, fruit enlargement, etc. It was clarified by research conducted at the molecular biological level that it was deeply involved in the metabolic system of reproductive growth, and it was developed into its application and utilization,
It has already helped to improve the quality of fruit such as taste, color and storability.

(3) Mixing and complexing of microbial ecosystem When the bacterial cells of the SK522 strain containing this yellow pigment are applied, the growth of general heterotrophic microorganisms, soil effective bacteria, etc. that propagate using it as a substrate is promoted. More importantly,
Apart from SK053 bacteria, thermophilic microorganisms such as SK522 strain do not immediately proliferate and activate in the field such as naturally occurring compost or locally concentrated solid fermentation such as blur fertilizer. . Initially, general room temperature heterotrophic microorganisms grow and the fermentation heat due to these actions is accumulated to help increase the product temperature, and only a single strain of thermophilic fibrinolytic bacteria and a small number of microorganisms are available. , It is not involved in the humus ripening of organic materials. It is required to mix and diversify the microbial flora. It is desirable to have a complex content that there are many effective bacteria of various types and that "feed (substrate)" is also appropriate. Thus, in the high-temperature decomposition of organic substances, bacterial flora mainly consisting of SK053 and SK522 strains are formed first, and the humus aging process not only involves simple component changes of constituents, but also extremely complicated microbial flora mutual interactions. The actions and their transitions proceed most efficiently with deep involvement. SK053 bacterium, SK5
Effective strains other than the 22 strain and the SK542 strain include the following.

(1) Culture of Hemicellulose Degrading Bacteria Hemicellulose forms a plant (cell wall) together with fibrin, and is called xylan, araban, dextran, mannan, galactan, etc., depending on the sugars constituting the plant. As a culture of hemicellulose-decomposing bacteria, Iwata's medium (1936) added to a concentration of rice xylan about 1% was used, and the cells were facultatively and anaerobically collected at 30 to 38 ° C. Usually Bacterium vulgatus, Bac. Prodigiosum, Bacteria mesentericu
s ruber), Microspyra Agariquefisense (M
A mixed culture of one or more strains such as icrospira agerliquefaciens) is obtained. (2) Cultures of pectin substance-degrading bacteria Many of the bacteria that strongly decompose pectin substances belong to the Bacillus subtilis group bacteria and ethanol / acetone bacteria when they are aerobic, and to the lactic acid bacteria when they are anaerobic. In the present invention, a Morish medium (Molisch 1939) is used, and soil, compost, horse manure, and spoilage such as bagasse and chomp are used as a separation source.
The inoculum is obtained at 27 to 35 ° C., 3 to 5 days of culture, and several rounds of culture in thick and thin layers. In the present invention, the culture of one or more strains is used.

(3) Soil actinomycete culture It is generally difficult to say about the action of actinomycetes (Actinomycetales Buchanan, 1917) in soil. But,
It decomposes various organic substances, especially incombustible fibrin and lignin, together with other microorganisms, and plays an important role in the formation of humus, which is the source of soil fertility. Certainly, it has important significance in terms of microflora control. For the culturing of actinomycetes, powerful bacteria are collected by using Waxman's medium (Waksman 1919), fertile soil as a separation source, and manure / manure. (4) Cultures of soil filamentous fungi and yeasts The most abundant locations of soil filamentous fungi are soils like bacteria and actinomycetes, and filamentous fungi in soils are naturally abundant in cultivated soil with plant roots, especially rhizosphere. Then, the work is also active. It is involved in the decomposition of organic substances such as plant remains and is related to soil fertility. It is considered that filamentous fungi are mainly active in the initial stage of decomposition. Next, there are many unclear points about the function of soil yeast. However, there are quite a few yeasts in the soil,
In addition, it is certain that the abundant vitamins and growth factors contained therein will affect coexistence with other microorganisms and soil activity. Cultures of soil filamentous fungi and yeast are separated and cultured from soil or manure / manure using a Tuapeck Dox medium (Czapek & Dox1910).

(5) Culture of thermophilic Bacillus genus Bacillus, which is generally aerobic, motile and has endospores, is a group of bacteria widely distributed in the natural world such as soil, leaf surface and hay,
It is also reported that most of the bacterial strains have strong heat resistance and can grow even at 55 ° C. or higher, and thus are major microorganisms during compost production. In addition, secreting antibacterial substances such as bacitracin and basilicin has attracted attention in the field of soil plant pathology in recent years. From these reasons, it is possible to try to utilize this bacterial group for crop production, and fertilized soil or a suspension of manure / manure is heat treated at 80 ° C. for 10 minutes to be used as a separation source. Waxman's medium (Waksman, 19
22), and aerobically collect the bacterial group at 50 to 60 ° C. In the present invention, single or mixed culture of these bacteria is used.

(6) Culture of yellow pigment-producing bacterium The yellow pigment produced by bacteria is a carotenoid pigment. There are many types of ripe compost, and many types. It is also often found on the leaves of plants. Using these as a separation source, a normal meat extract medium or peptone-yeast extract medium,
Incubate and separate at 25-35 ° C under aerobic conditions. It is easily identified by colors such as yellow-green, yellow, yellow-brown, and red, and is generally flavobacterium (Flavobacterium), chromobacterium (Chromobacterium), pseudomonas (Pseudomonas), serratia (Serratia), phototrophic bacteria (Phototrophic bacteria) A culture of one or more strains belonging to, etc. is obtained. And next, SK0 of the present invention
The SK522 strain mixed with 53 strains and the SK542 strain having a favorable influence on the growth and fermentation of the mixed culture will be described.

[I] SK522 strain Clostridium thermocellum biovar SK522, Microtechnical Laboratory Article 3459, FERM BP-3459 (abbreviation SK522 strain) Scientific properties Bacteriological properties Cannot grow alone without companion bacteria. However, since the associated bacteria are easily isolated and pure-cultured, it is the result of testing various properties of the isolated associated bacteria while co-culturing them with the isolated associated bacteria as a basis. Shape There are also straight rod shape and slightly curved shape. Alone, sometimes two. 0.3-0.5 × 2.2-4.0 μ. When the culture becomes old, it extends into filaments, long-chain, and lengths of 5.7 to 12.8.
μ. Motility is not observed in pericardium and room temperature hanging drop specimens. Oval or circular spores are formed at the ends to expand the cells and form a club. Gram negative.

Culture properties (1) Plate culture It does not grow on plate culture using broth agar or other commonly used medium. However, while the medium presented by Viljoen et al., Tetrault circular filter paper agar plate forms colonies with associated bacteria and yellow spots, it cannot be produced by this bacterium alone. (2) Slope culture and stab culture Addition of Schweizer reagent-treated filter paper to Viljoe et al. Medium Agar slope culture and stab culture do not grow. (3) It does not grow on potato culture, gelatin culture surface, or puncture. (4) Liquid culture medium of Viljoen et al., Fibrin gravy,
Growth is observed only in a medium containing fibrin such as fibrin peptone water (poor growth). If a substance other than fibrin such as glucose or xylose is used as a carbon source, the decomposing power of fibrin is lost.

Physiological and biochemical properties 1. Enzymatic action, etc. (1) Fibrinolysis The fibrinolytic enzyme possessed by this bacterium is essentially composed of some β
It is a complex of -1,4-glucanase. Secreted extracellularly, action temperature 80 ℃ or more, same hydrogen ion concentration pH = 1
It was confirmed that several kinds of enzymes having heat resistance and alkali resistance of 0.0 or more were contained. It cleaves from the ends of macromolecules of fibrin that form a micellar structure to produce glucose, cellobiose, cellooligosaccharides and the like. When the substrates that act on these enzymes are mainly shown, it decomposes filter paper FP-ase: Positive Avicel is decomposed Avicelase: Positive Cellobiose is decomposed into two molecules of glucose Cellobiase: Positive (2) Lignin-decomposed Inawala lignin solubilization : Positive (weak, weak) (3) Proteolytic enzymes: Negative peptidase: Positive (4) Starch hydrolysis test: Slightly positive (5) Hemicellulose, xylan, pectin hydrolysis test : Negative (6) Invertase, Maltase: Positive (7) Lipolysis test: Negative (8) Oxidation reaction Hydroquinone reaction: Positive Tyrosine reaction: Negative (9) Reducing action: Positive

2. Product Test (1) Fibrin Fermentation Fermentation rate 78-91%, vigorous fermentation of fibrin, ethanol, methanol, acetaldehyde, acetic acid, lactic acid, formic acid, lactic acid, succinic acid, fumaric acid, tartaric acid, gluconic acid, It produces glucose, cellobiose, cellooligosaccharides, cellodextrin, large amounts of carbon dioxide, hydrogen, and hydrogen sulfide. (2) Produced acid from other sugars and alcohol Produced acid from glucose, sucrose, maltose, and cellobiose. (3) Gas generation test Although gas is generated more violently than fibrin, no gas generation is observed from glucose, sucrose, maltose, and cellobiose. (4) Peptone water test Ammonia: Slight reaction Indole: Positive skatole: Positive Hydrogen sulfide: Positive (5) Pigment production Usually, carotenoid yellow pigment production 3. Growth conditions (1) Growth temperature Optimum temperature is 65-72 ° C, temperature range is 40-80 ° C, 4
It does not grow below 0 ° C. (2) Hydrogen ion concentration Optimum hydrogen ion concentration pH = 6.7-8.0, the range is 5.6-9.6. (3) Nitrogen source Peptone is the best, and urea, uric acid, asparagine, sodium glutamate, etc. are also good. Ammonium salts are also good sources of inorganic nitrogen. (4) Carbon source When the subculture is continued with carbohydrates other than fibrin, its growth and fermenting power are lost. (5) Relationship with oxygen Eh = 200 to −250 mV, which is an anaerobic bacterium. (6) Requirement of micronutrients Micronutrients such as biotin, pyridoxamine, vitamin B12 and p-aminobenzoic acid are required. 4. Content of G + C of DNA mol% of G + C = 38-40 (Tm) is estimated.

II. Taxonomic position This SK522 fermenting fibrin vigorously at the optimum growth temperature of 65 to 72 ° C and temperature range of 40 to 80 ° C.
The following thermophilic fibrinolytic bacteria are similar to the strains. Clostridium ther
mocellum Viljoen, Fred and Peterson, 1926 .: Jour. A
gr. Sci. (London), 16,7 (1926).) C. dissolvens
Bergey et al. 1925, aka Bacillus cellulose dessolve
ns Khouvine, 1923 .: Ann. Inst. Past.37,711 (1923); Be
rgey's Manual, 2nd.Ed. (1925) p.344.) C. thermocellulas
eum Enebo, 1951.: Bergey's Manual, 7th.Ed. (1957) p.68
9.) Bacillus thermocellolitex
ellulolyticus Coolhaas, 1928.: Cent Bakt.II, 75,101
(1928), 76,38 (1929).) Bacillus thermofibrinco
lus Itano and Arakawa, 1929: Farming, 5,816,921 (1929); 6,
248,257 (1930).) Among these, Clostridium disorbens is a bacterium that is very similar to this strain in morphological, culture, and physiological biochemical tests except for the following four differences. (1) It has a lignin-solubilizing ability although it is weak or weak. (2) Suitable growth temperature of 65 to 72 ° C, growth temperature range of 40 to 80
Does not grow at temperatures below 40 ° C. (3) Actively ferment fibrin, but not hemicellulose, xylan, pectin, etc. (4) Lignin solubilization ability is the genus Thermus
It is markedly symbiotically emphasized by certain bacteria of the same type, and at the same time, has a favorable effect on the fibrinolytic activity and other action functions of this bacterium. However, at present, Berges' Manual (Bergsy's Manual
of Systematic Bacteriology Vol.2 (1986) p.1104, p.11
In 41.), only Clostridium thermocellum is described as a thermophilic degrading bacterium. All of them are treated as subspecies or variants of Clostridium dissolvens, or incompletely studied. Therefore, for the classification and identification of this strain, it was decided to compare and examine Clostridium thermocellum and various mycological properties thereof, and at the same time, other thermophilic fibrinolytic bacteria were also compared for reference.

In summary, the results of comparative examination of various mycological properties as described above show the characteristic properties of this strain and known thermophilic fibrinolytic bacteria including Clostridium thermocellum. The differences are the above-mentioned four items, and they can be enumerated in various ways such as the action on various other carbohydrates, the requirement of micronutrients, etc., but what I would like to emphasize especially is the problem with lignin. This strain has a lignin solubilizing ability, and the solubilizing ability is thermus aquaticus SK542 (Thermus aquaticus bi
It is significantly enhanced by the symbiotic mixed culture with ovar SK542). In contrast, there is no description about lignin of other thermophilic fibrinolytic bacteria including Clostridium thermocellum. If so, it is about its inhibitory effect on fibrin degradation. For this reason, a new strain (Strain) was established based on the physiological and biochemical properties of the lignin-solubilizing ability of this strain as one basis, and Clostridium thermocellum SK522 (Clostridium thermocellum biova
r SK522). This strain as a standard strain,
Clostridium ther
mocellum Viljoen, Fred and Peterson, 1926.) has the ability to solubilize lignin and is of the genus Thermus (genu
s Thermus Brock and Freeze 1969), which is characterized by significantly enhancing the lignin solubilizing ability by symbiotic mixed culture with a certain bacterium belonging to the SK522 strain and natural and artificial mutants It is a new strain depending on the physical properties.

Microbial Accession Number The microorganism accession number of this strain is Microtechnology Research Institute No. 3459 (FERMBP-3459).

Screening of SK522 strain Soil, beach sludge, manure / manure, human / livestock feces were isolated sources, and after repeated concentrated culture several times at 55-65 ° C., presented by Viljoen et al. Medium Tetrault circular filter paper Agar plates are used for isolation in a conventional manner. Viljoen et al. As medium
The salt composition presented by is suitable, but if necessary, a trace amount of inorganic metal salts, vitamins, growth promoting factors such as yeast extract and the like may be added. Viljoen, Fred and Peterson 1926 Peptone 5.0 g Calcium carbonate Excess ammonium phosphate sodium 2.0 g Potassium acid phosphate 1.0 g Magnesium sulfate 0.3 g Calcium chloride 0.1 g Ferric chloride Trace fiber Element (filter paper) 15.0 g Imizu 1000 ml

[II] SK542 strain Thermus aquatics SK542 (Thermus aqua
ticus biovar SK542, Micro Engineering Research Article No. 3382,
FERM BP-3382) (abbreviation SK542 strain) I. Scientific properties Mycological properties Form Long rod-shaped, 0.4-0.6 × 3.0-5.0μ. Under certain conditions, for example, when culture becomes old, filamentous, length 20 to 130
μ. No flagella and no motility in room temperature hanging drops.
No endospores. Gram negative.

Culture property Proliferation is active. Generation time is 20 to 50 minutes. (1) Plate culture 3% agar, 60 ° C culture: yellow, relatively dense, small round colonies. (2) Agar stab culture Only surface growth, yellow slightly expanded. (3) Liquid culture Grows in a film on the surface (static culture).

Physiological and biochemical properties 1. Enzyme action, etc. (1) Proteolytic proteolytic enzyme: Positive (strong) (proteolytic enzymes) Gelatin hydrolysis: Positive peptidase: Positive (2) Starch hydrolysis: Slightly positive (3) Pectin degradation: Positive (weak) ) (4) Degradation of fibrin: Negative (5) Degradation of lignin: Negative Degradation of both fibrin and lignin is negative, but lignin digestion of SK522 strain is possible by symbiotic mixed culture with thermophilic fibrinolytic SK522 strain. It significantly enhances the solubilizing ability and, at the same time, positively affects the fibrin decomposing power and other action functions. (6) Lipolysis: Negative (7) Invertase, maltase: Positive (8) Catalase, oxidase reaction: Positive (9) Hydrogen sulfide production: Positive (weak) (10) Sulfate reduction reaction: Negative 2. Product test (1) Raw acid and gas generation from sugar and alcohol No gas generation. A raw acid from glucose, galactose, maltose, lactose, and glycerol. (2) Formation of indole and skatole: Negative (3) Pigment production Yellow pigment (carotenoid pigment, maximum absorbance 450n
m, others with small peaks at 430, 435, 470 nm). However, it is scarcely produced in a synthetic medium containing glucose and other sugars as a carbon source.

3. Growth conditions (1) Medium concentration Bacteria of the genus Thermus are generally sensitive to organic matter concentrations. It is said that the less nutrients and the lower the concentration, the better. However, this strain is different from these in that it grows well even at normal concentrations. It grows even with salt NaCl 5% or more. Optimal concentration 2.
0-3.0%. In general, Genus Thermus bacteria do not grow in the presence of more than 2% NaCl. It grows even with sodium glutamate of 3% or more. It grows on sucrose or maltose 5% or more. Its growth is not inhibited even in the presence of 2% peptone + 1% yeast extract. Generally the genus Thermu
For the bacteria of s), trypton and yeast extract concentrations of about 0.1% are suitable, and they do not grow when they exceed 1%. (2) Growth temperature Optimum temperature is 72-76 ℃, growth temperature range is 40-82 ℃,
It cannot grow below 40 ° C. (3) Hydrogen ion concentration Optimal hydrogen ion concentration pH = 6.0 to 10.0. Growth hydrogen ion concentration range pH = 4.0 to 11.0. (4) Nitrogen source Proteins such as gelatin, glutamate, urea, and ammonium salt are often used as an inorganic nitrogen source. (5) Carbon source Glucose, sucrose, maltose, galactose, cellobiose, raffinose, stachyose, starch, glycerol, acetic acid, lactic acid, malic acid, glutamate are used. (6) Relationship with oxygen Absolutely aerobic. (7) Demand for micronutrients This strain has high demands for micronutrients. In addition to vitamins such as biotin, nicotinamide, and thiamine, a relatively high concentration of metal ions such as iron, manganese, and calcium is required for good growth of this strain. It is also sensitive to the amount of these minerals. (8) Susceptibility to antibiotics Like general Thermus genus (genus Thermus) bacteria,
It shows high sensitivity to penicillin G, chloramphenicol, tetracycline, streptomycin, kanamycin and other antibiotics. 4. Content of G + C of DNA mol% of G + C = 68 (Tm) is estimated.

II. Taxonomic position The bacterium does not grow in absolute aerobic condition, optimum growth temperature of 72-76 ° C, growth temperature range of 40-82 ° C, 40 ° C or lower. Since it is a non-spore-free, Gram-negative long bacillus, etc., in consideration of other mycological properties, the genus Thermus (genus Thermus Broc
k and Freege 1967). And, as a bacterium of similar Genus Thermus, Thermus aquaticus Brock and Fr
eege 1969.:Bergey's Manual (1984) Vol.1, P.337.) Thermus thermophilus Osh
ima and Imahori 1974.:Int.J.Bacteriol.,24,102(197
4)) Thermus Flavus Saiki, Kimura an
d Arima 1972.:Agr.Biol.Chem.,36,2357(1972)), etc., but currently Bersey's Manual
s Manual of Systematic Bacteriology Vol.1 (1984) P.33
3) Genus Thermus Brock and Freeg 196
Bacterial species belonging to 7) are Thermus aquatics (The
rmus aquaticusBrock and Freege 1969),
Moreover, since it has characteristic properties that cannot be found in the known bacterial species as described below, this strain is used as a new strain (strain) of Thermus aquaticus SK542 (Thermus aquaticus biovar SK5
We conclude that it is reasonable to name it 42).

(1) The lignin-solubilizing ability is remarkably enhanced by symbiotic mixed culture with the thermophilic fibrinolytic bacterium SK522 strain. At the same time, it has a positive effect on the ability to decompose fibrin and other functions. (2) It has a strong proteolytic activity with a wide range of working hydrogen ion concentration and working temperature. (3) The genus Thermus (genus
(Thermus) bacteria are very weak and grow well in medium of normal concentration. (4) Strong demand for micronutrients, demand for various vitamins and metal ions, and sensitivity to their mineral contents. (5) Yellow pigment (carotenoid pigment, maximum absorbance 45)
0 nm and others have small peaks at 430, 435 and 470 nm).

The SK542 strain is a standard strain of this strain, and the Thermus aquaticu (Thermus aquaticu)
s Brock and Freege 1969), by symbiotic mixed culture with a thermophilic fibrinolytic bacterium (SK522 strain), its lignin solubilizing ability was remarkably enhanced and at the same time, the fibrinolytic activity was also affected. And a new strain (strain) having physiological and biochemical properties that includes the SK542 strain and natural and artificial mutant strains. Microbial Accession Number The microorganism accession number of this strain is Microtechnology Research Institute Article No. 3382 (FERMBP-3382).

Screening of SK542 strain Isolate sources are hot spring sources in and around Kyushu, soils near hot spring sources, humus, and the like. The separated sample is precultured at 55 to 60 ° C. and then isolated by 3% agar plating according to a conventional method. Separation medium composition (Castenholz medium: Castenholz, RW; Bacteriol.
Rev., 33, 467 (1969)) Nitrilotriacetic acid 100 mg CaSO4 · 2H2O 60 mg MgSO4 · 7H2O 100 mg NaCl 8 mg KNO3 103 mg NaNO3 689 mg Na2HPO4 111 mg FeCl3 0.28 mg MnSO4 · H2O2 H2O3H0O 0.016 mg Na2M0O4 · 2H2O 0.025 mg (yeast extract 5000 mg) (tryptone 5000 mg) (sucrose 10000 mg) Total amount (pure water, pH = ~ 8) 1000 ml Note: The composition in () is modified by the inventors. Example of application This section describes an example of actual fertilization management by applying individual fermented soil activator. For pine needle bed soil, straw rod floors, and sashimi, a symbiotic mixed culture of SK053 and SK522 strains is mixed with excipients, micronutrients and minerals, and if necessary, selected fibrinolytic bacteria. In addition to the above, one of the application methods is to mix useful microorganisms and spray them as they are. Application example 1. Blurred manure Blurred manure is a fertilizer that is fermented by mixing organic matter such as oil dregs, rice bran, fish dregs and meat dregs with mountain soil and viscosity. But that's it. Once oil dust or fish meal is applied as it is raw and watermelons and cucumbers are spread directly, it seems that it was born as a measure to prevent the growth damage and insect damage such as gall flies caused by it. Mix with etc. to fertilize and make it glow and blur. It seems that he was said to be blurry from such a place. It has been around for a long time. [Ingredients] Generally, there are a wide variety of ways to make and use blurred manure, and there are a wide variety of ingredients. However, there are some points in creating a good blur. The first is selection and blending of raw materials. Next, it is basically necessary to add a suitable amount of mountain soil or a substance having a large solar ion exchange capacity such as viscosity to the various organic substances. In addition, it is important to mix well the organic compounds of raw materials with those with a large amount of nitrogen and those with a large amount of phosphoric acid. Especially, rice bran contains a large amount of phosphoric acid and has a good balance of nutrients and various micronutrients. Therefore, it is highly valuable as a growth promoter for soil-effective bacteria. Now, one example of the raw material blends that the present inventors often apply is as follows. [Raw material blend] Oil residue 50 kg Superphosphate 3 kg Calcium carbonate 10 kg Dry chicken manure 50 kg Rice bran 60 kg Bone meal 7 kg Rice bran 5 kg Potassium chloride 5 kg Zeolite 10 kg ──────────────────── Total 200 kg Mixed culture of these new bacteria 053 and fibrinolytic bacteria (00
(See paragraph 27) Add 2.0 liters and mix well, water content approx. 4
Adjust to 07%. [Fermentation] Firefighting and aging of organic substances are all solid fermentations.
Main fermentation is 5 to 7 days, maximum fermentation temperature is 65 to 80 ° C, and post-ripening fermentation is 10 to 14 days. [Applying] Topical application is the principle. Basically, it is applied not only to the entire surface of the planting hole or by digging a groove, but also along the area where the root is stretched. Even if the concentrated fertilizer is locally applied, the concentration damage to the roots is unlikely to occur, and rather the part has better air permeability, good rooting, and efficient nutrient absorption. [Effect of application] Field cultivation test of cucumber Variety: Tsubasa (Takii Inoculum Co., Ltd.) Field: Black soil, volcanic ash soil. Fertilization: Control group, Granulated chemical fertilizer per 1 m (10-10
-10) 300 g. Blurred fertilizer plot, 500 g of blur fertilizer per 1 m 2, and the amount of fertilizer applied in the control plot was adjusted to the same amount by adding ammonium monosulfate, lime superphosphate, and potassium chloride. And a part 10 of blur manure
An additional fertilizer of 0 g is irrigated into the plant. No sterilization operation before fertilization. Allocate 6m each, and plant 15 cucumbers on them on March 5th. The results of the cucumber cultivation test are shown in Tables 2 and 3. Table 2 Cucumber yield per 6 m (15 shares) ──────────────────────────────────── Section 5 5th of June 6th of June Harvest until harvest of total weight ─────────────────────────────────── ─ Counter ward 63 153 197.87Kg Blurred fertilizer ward 85 267 40.48Kg ────────────────────────────── ────── Table 3 Soil microbial flora ───────────────────────────────── Micro organisms Blur control Section ───────────────────────────────── Aerobic bacteria × 10 ⁶ / g 16.3 2.7 (including Facultative anaerobes) Actinomycetes × 10 ⁶ / g 25.3 0.1 Filamentous bacteria × 1 0 ⁴ / g 6.5 2.6 thermophilic fibrin-degrading bacteria × 10 ³ / g 4.9-─────────────────────────── ──────── M. P. According to the N method. The cucumber leaves in the blur area are thick, the internodes are clogged, and the thorns are painfully sharp. The harvested cucumbers are dark and have a long life. Moreover, the yield is 1.7 times, which is a large harvest. In addition, the soil microbial flora has more numbers and types in the blur group than in the control group, indicating that it is stable. Application example 2 Koji There is a method called "Koji" to make good use of soil in organic farming. It aims to improve and activate the soil as a whole, rather than the direct effect as a fertilizer. In particular, it may show remarkable effects in improving soil microflora. In the invention of the present application, the soil is a fungal fungus (Aspergillus) genus strain, which is inoculated and fermented with a reference to page 0035, and is a collection of filamentous fungi, actinomycetes, bacteria, etc. mainly composed of fungi. . [Ingredients and compounding] Soil 1000 kg Rice bran 30 kg Zeolite 10 kg A culture of a new bacterium 053 and a thermophilic fibrinolytic bacterium (SK522 strain) in which soil mould mold (Aspergillus sp.) Is added (see paragraphs 0027 and 0028). Add 20.0 l to adjust the water content to around 40%. [Fermentation] The fermentation temperature is 38 to 42 ° C, the main fermentation is 5 to 7 days, the maximum fermentation temperature is 40 to 42 ° C, and the post-ripening fermentation is 10 to 14 days. [Application] The sandbag of the present invention is 1 per 10 ares.
Apply ~ 2000kg every year or once every 2-3 years. [Application effect] Field cultivation test of peppers Varieties: Kyonami (Takii Inoculum Co., Ltd.) Field: Black soil, volcanic ash soil Fertilization: Soil and fertilizer plots, 300 g of granulated fertilizer (10-10-10) per 1 m of control plots. Then, 1 kg / m 2 of sandbags is sprayed and placed in the sandbag area before planting. Allot 6m to each plant and plant 10 green peppers on them on March 15th. The results of the cultivation test of peppers are shown in Table 4. And Table 5. It is as follows. Table 4 Yield table by pepper quality (average per strain) ──────────────────────────────────── Good result Deformation result ──────────────────────────────────── Control section 209.4 This 5235.0 g 10.5 this 185.3g soil mound 252.4 7824.4g 5.7 62.2g ─────────────────────────── ────────── Table 5 Soil microbial flora ─────────────────────────────── Microbial blur control Section ─────────────────────────────── Aerobic bacteria (including facultative anaerobic bacteria) × 10 ⁶ / g 8. 2 1.4 Actinomycetes × 10 ⁶ / g 11.3 0.4 Filamentous fungus × 10 ⁴ / G 105.0 0.0 Thermophilic fibrin-degrading bacteria × 10 ³ / g 3.6-──────────────────────────── ──── M. P. According to the N method. The higher amount of ash and higher yield in the soil application area than in the control area seems to be due to the fact that the application amount of organic fertilizer was not so high in the test field in the past, but in this test , Clearly showing a good application effect.
In addition, I think this is supported by the measurement results of thermophilic fibrinolytic bacteria.

[0054]

INDUSTRIAL APPLICABILITY As described above, the present invention is a novel strain SK0
The successful symbiotic co-cultivation of 53 with fibrinolytic bacteria such as the new strain SK522 has enabled several excellent uses of the system of action of microbial flora in natural and agricultural ecosystems as well as factory production systems, Provided is a solid fermenting soil activator that ensures its practical application. Appropriate application of the soil activator of the present invention improves the quality of cultivated plants,
High yield can be raised as the first feature, and further, the application of the solid fermenting soil activator as in the present invention is (1) Fertilization effect of the applied organic substance which does not give concentration disturbance to the plant root due to fermentation. (2) Efficient utilization of fertilizer components (3) Stabilization of soil microflora and prevention of pests and diseases are drawing attention. The above effects are unthinkable with conventional fertilizer-based fertilizer and fertilizer application techniques, and are not the only slow effects of organic fertilizers. Of course, it's also different from compost. Then, what should be considered about the effect of the excellent solid fermenting soil activator of the present invention, it requires a so-called change of idea. In other words, it is to give citizenship to soil microorganisms that have been ignored in the past. Soil microorganisms will be taken up as one of the important factors in conventional fertilization techniques and cultivation theory. Application effect of the solid fermenting soil activator of the present invention, root-microorganisms-by applying agglomerates of effective microorganisms centering on thermophilic heat-dissipating bacteria around the cultivated plant.
The soil-trilateral relationship was activated, and the soil, especially the rhizosphere, became healthy.

─────────────────────────────────────────────────── ─── Continuation of the front page (51) Int.Cl. ⁶ Identification code Internal reference number FI technical display location C12R 1: 145) C09K 101: 00

Claims (2)

[Claims] 1. A thermoactinomyces genus, which comprises 10
A thermophilic actinomycete Thermoactinomyces SK053 sp. Nov. (Having a wide range of growth temperature of 85 ° C., a wide range of growth hydrogen ion concentration of 5.3 to 10.8 pH, a lignin solubilizing ability and a fibrin decomposing ability) Thermoactinomyces SK053
sp. nov., Institute of Biotechnology, Institute of Biotechnology, Contract No. FERM P-13598), and an individual fermented soil activator whose main active ingredient is a symbiotic mixed culture of other fibrinolytic bacteria. 2. The other fibrinolytic bacterium according to claim 1, wherein the thermophilic fibrinolytic bacterium Clostridium thermocellum SK.
522 (Clostriduim thermocellum biovar.SK52
2, Fermentation Soil Activator, which is Mikori Kenjoyori No. 3459).