JPH08108096A - Vessel for centrifugal separation - Google Patents

Abstract

PURPOSE: To eliminate the need to transfer a processing soln. to a microtube from a vessel for centrifugal separation unlike the conventional process by forming a small-capacity part with the content volume specified separately under a large-capacity part with the content volume specified to constitute the vessel. CONSTITUTION: A small-capacity part 3 with the content volume set at 0.5-5ml is separately formed under a large-capacity part 2 with the content volume set at 5-100ml to constitute a vessel 1 for centrifugal separation. In this case, the large-capacity part 2 and small-capacity part 3 are integrally formed, an annular groove 4 is formed on the periphery so that the vessel thickness at the boundary A is smaller than that of the other part to snap off the large- capacity part 2 and small-capacity part 3 from each other, and annular flanges 5 and 6 are formed respectively above and below the groove 4. Further, the inner wall of the vessel close to the boundary A is tapered so that the inner diameter of the vessel is increased from the bottom toward the upper part. As a result, the transfer of the processing soln. from the vessel to the microtube is not needed unlike the conventional process.

Description

Detailed Description of the Invention

[0001]

The present invention relates to cytology, cell immunology,
Centrifugation to efficiently perform centrifugation / washing, cell preservation, gene extraction, etc. when separating various cell components such as lymphocytes, stem cells, malignant cells of blood or tissue in the field of molecular biology analysis etc. For containers.

[0002]

2. Description of the Related Art Conventionally, when separating various cell components by a centrifugation method, it is generally rare to store or process cells in a centrifuge container having an internal volume of 10 to 15 ml or 50 ml. An operation of temporarily transferring the treatment liquid from the separation container to a microtube having an internal volume of 0.5 to 1.5 ml is performed.

In this operation, a large-capacity centrifuge container is used to repeat centrifugation and washing a couple of times, the supernatant is discarded, and then the precipitate remaining in the centrifuge container is used. For some cell pellets, again use a small amount of solvent (much less than the amount used to wash 1.25-1.5
ml) to loosen the cells, transfer the cell suspension to a microtube using a pipette, centrifuge the cell suspension in the microtube, and make a new cell pellet in the microtube. There is. Then, this cell pellet can be stored as it is, or can be frozen and stored in a frozen preservation solution, or directly from the cell pellet.
We are working to extract NA and RNA.

[0004]

As described above, in the conventional method of transferring the processing liquid from the centrifuge container to the microtube, the cell suspension must be prepared only for transferring the processing liquid. Therefore, the number of operations for loosening the cell pellet increases once.

Further, there is a problem that cell damage is increased due to the fact that after the cell turbid solution is transferred to the microtube, centrifugal separation must be added to make the cell pellet again.

When transferring the cell turbid solution to the microtube, a high-concentration cell turbid solution disintegrated in 1.25 to 1.5 ml of the solvent is processed, so that the centrifuge container before transfer is treated. There is a problem that a large amount of cells adhere and remain on the inner wall and the inner wall of the pipette used for transfer.

Further, there are problems that it takes time and labor to perform the transfer operation and that space is taken up in the case of storing in a centrifuge container.

Further, in the conventional centrifuge container, when the supernatant is discarded after centrifugation, there is a problem that a part of the cell pellet, which is a precipitate, flows out together with the supernatant.

In view of the above problems, the present invention eliminates the need to transfer a large-capacity container to a small-capacity container, and
An object of the present invention is to provide a container for centrifugation in which a cell pellet does not easily flow out when discarding a supernatant after centrifugation.

[0010]

In order to achieve the above object, the centrifuge container of the present invention has an internal volume of 5 to 100 m.
It is characterized in that a small volume part having an internal volume of 0.5 to 5 ml is separably formed below the large volume part of 1 l.

As means for forming the large-capacity part and the small-capacity part in a separable manner, the large-capacity part and the small-capacity part are integrally molded, and the container at the boundary between the large-capacity part and the small-capacity part is formed. An annular groove may be provided on the outer periphery so that the wall thickness is thinner than other portions, so that the large-capacity portion and the small-capacity portion can be formed so that they can be fractured and separated. Further, annular flanges can be formed directly above and just below the annular groove.

Further, as a means for forming the large capacity part and the small capacity part in a separable manner, the large capacity part and the small capacity part are separately molded, and the lower end part of the large capacity part and the small capacity part are formed. It is possible to provide a screw to be screwed with the upper end of the portion, and the large capacity portion and the small capacity portion can be separably connected by the screw.

Further, as means for forming the large-capacity portion and the small-capacity portion in a separable manner, the large-capacity portion and the small-capacity portion are separately molded, and the lower end portion of the large-capacity portion and the small-capacity portion are formed. Form an annular flange on the upper end of the part,
The large-capacity portion and the small-capacity portion may be separably connected to each other by fitting a locking tool to the peripheral portion where the two flanges are butted against each other.

Here, the inner wall of the container near the boundary between the large-capacity part and the small-capacity part can be tapered so that the container inner diameter increases from the container bottom to the container upper part.

Further, it is possible to form a concave portion or a convex portion at one or more locations on the outer circumference of the lower root portion of the annular flange of the small capacity portion. When forming recesses or protrusions at multiple locations, the shapes of the recesses or protrusions may be changed, or the recesses or protrusions may be arranged at specific positions, etc. It is desirable to be able to do so.

The optimum combination of the internal volume of the large volume part and the internal volume of the small volume part can be determined depending on the treatment liquid and the treatment method.

It is desirable that the length of the small capacity portion is 50 mm or less. When the thickness is 50 mm or less, when the supernatant liquid is discarded after centrifugation, the supernatant liquid is smoothly discharged without remaining in the small volume portion due to the surface tension.

The material of the container is preferably synthetic resin from the viewpoint of material cost and easiness of molding, and it is desirable that the container is transparent or translucent so that the state of the processing liquid inside can be easily grasped.

A scale may be provided on the outer surface of the container so that the contents can be easily understood, and a screw for attaching a lid can be provided on the upper end of the container.

[0020]

According to the centrifuge container of the present invention, when centrifuging / washing cells, the cell pellet remaining at the end of centrifuging / washing remains from the beginning as a cell pellet at the tip of the small volume portion. There is no need to transfer to a microtube by using a pipette as in the case of using the centrifugation container of.

When the inner wall of the container near the boundary between the large volume part and the small volume part is tapered so as to widen toward the upper part of the container, the supernatant liquid is discarded when the supernatant liquid is discarded after centrifugation and washing. Is smoothly discharged without remaining in the small capacity part.

Furthermore, after discarding the final supernatant, the large volume part and the small volume part can be separated, and the separated small volume part can be used as an independent small container for centrifugation.

In the case of a container in which the large-capacity part and the small-capacity part are integrally molded, when the large-capacity part and the small-capacity part are broken and separated, the container thickness at the boundary between the large-capacity part and the small-capacity part is larger than that of the other part. Since the annular groove is provided on the outer circumference so as to be thin, the fracture separation is easy. Since the upper and lower portions of the thin wall portion are reinforced by the annular flanges, there is no problem in strength when handling the container and applying it to the centrifuge.

The annular flange formed on the upper end of the small volume portion functions as a locking member for the vessel when the small volume portion after being separated from the large volume portion is set as a small container in the centrifuge. . Further, the concave portion or the convex portion formed on the lower root portion of the annular flange has a function as an alignment mark when the small volume portion after separating from the large volume portion is set in the centrifuge as a small container, and , Has a function as a locking projection of a fixing projection or a notch of a lid to be attached later to the opening of the small capacity portion.

By confirming the marker for alignment and fixing the lid to be attached later, it is a precipitate when the supernatant liquid is discarded after centrifuging and washing with a small volume part after separation as a small container in a centrifuge. It is possible to prevent a part of the cell pellet from flowing out together with the supernatant.

[0026]

1 is a front view showing a centrifuge container according to a first embodiment of the present invention, FIG. 2 is a vertical sectional view of a portion A in FIG. 1, and FIG. 3 is a view showing a lid of the centrifuge container. Partial cutaway front view, FIG. 4 is a view showing a state when the supernatant is discarded, FIG. 5 is a view showing a state of breaking and separating the centrifuge container, and FIG. 6 is a retrofit to the opening of the small container after the breaking and separating. FIG. 7 is a perspective view showing a lid, FIG. 7 is a perspective view showing a state where the lid of FIG. 6 is attached to a small container after fracture separation, and FIG. 8 is a diagram showing a state where the small vessel after fracture separation is set in a centrifuge. FIG. 9 is a diagram showing a state of discarding the supernatant liquid from the small container after the fracture separation.

The centrifuge container 1 of this embodiment is made of a transparent synthetic resin, and has a large capacity part 2 having an inner volume of 12.0 ml and a small capacity part 3 having an inner volume of 1.5 ml at the bottom thereof in a tubular shape with a bottom. It is molded integrally with.

As shown in FIGS. 1 and 2, the boundary portion A between the large-capacity portion 2 and the small-capacity portion 3 is provided with a circumferential groove 4 on the outer periphery so that the container wall thickness at the boundary a is thinner than other portions. The large-capacity portion 2 and the small-capacity portion 3 are provided so as to be fractured and separable, and annular flanges 5 and 6 are formed immediately above and below the circumferential groove 4, respectively.

As shown in FIG. 2, the inner wall of the container at the boundary A between the large-capacity part 2 and the small-capacity part 3 has a smooth surface without steps so that the container inner diameter increases from the container bottom to the container upper part. It has a taper.

The large-capacity part 2 has an upper outer diameter of about 17 mm,
The outer diameter of the lower part is about 13 mm and the length is about 85 mm.
At the upper end of the large-capacity part 2, a screw-type lid 10 shown in FIG.
There is provided a screw 11 for mounting.

The small-capacity part 3 is continuous with the lower part of the large-capacity part 2 and tapers toward the bottom, and the outer diameter of the bottom is about 5 mm and the length is about 38 mm. Then, a peripheral groove 7 for mounting the lid 20 for retrofitting shown in FIG. 6 is formed in the lower root portion of the upper annular flange 6, and a concave portion 8 is formed at one position in the circumferential direction of the peripheral groove 7. ing.

This centrifuge container 1 can be broken and separated from the boundary a between the large-capacity part 2 and the small-capacity part 3.
The small volume part 3 after separation can be used as an independent small container for centrifugation.

At this time, when the large-capacity part 2 and the small-capacity part 3 are fractured and separated, an annular ring is formed on the outer circumference so that the container wall thickness at the boundary a between the large-capacity part 2 and the small-capacity part 3 becomes thinner than the other parts. Circumferential groove 4
Since it is provided, breakage separation is easy. The thickness of the boundary a is thin, but the upper and lower parts of the boundary are annular flanges 5, 6
Since it is reinforced by, there is no problem in strength when handling the container and applying it to the centrifuge.

The annular flange 6 of the small-capacity part 3 is set when the small-capacity part 3 separated from the large-capacity part 2 is set as a small container for centrifugal separation in the centrifugal separator 30 as shown in FIG. It functions as a locking member for the body.

The peripheral groove 7 formed in the lower root portion of the annular flange 6 of the small-capacity portion 3 is used as a small container for centrifugal separation after being separated from the large-capacity portion 2, and is attached afterward as shown in FIG. It is a groove into which the fixing wheel 21 of the lid 20 fits when the lid 20 for mounting is attached as shown in FIG.

Further, the recess 8 formed at one location in the circumferential direction of the circumferential groove 7 is a recess for engaging with the projection 22 formed on the fixing wheel 21 of the lid 20. By these fittings, the lid 20 is securely fixed to the small capacity portion 3 (small container), and the both do not rotate relative to each other.

A recess 8 formed at one location in the circumferential direction of the circumferential groove 7.
Serves as a marker for alignment when setting the small capacity part 3 (small container) in the centrifuge.

That is, when the centrifuge container 1 before being separated from the large-capacity part 2 is attached to the centrifuge, the concave portion 8 is set so as to be located on the outermost side. The cell pellet P remaining on the tip of the small-capacity portion 3 adheres to the outermost side, that is, the side where the concave portion 8 is formed. Therefore, when the small-capacity portion 3 (small container) after separation is set again in the centrifuge 30, if the concave portion 8 is set to be the outermost side, the attachment position of the cell pellet P does not change. .

As an actual work procedure, the small-capacity part 3 (small container) after separation is provided with the lid 20 for retrofitting shown in FIG.
Since the position of the connecting portion 24 of the lid 20 corresponds to the position of the concave portion 8 of the small capacity portion 3 (small container) when mounted as shown in FIG. 8, the small capacity portion 3 (small container) is shown in FIG. When the centrifugal separator 30 is set, the connecting portion 24 of the lid 20 may be set to the outermost position. That is, after the lid 20 is attached, the connecting portion 24 of the lid 20 also functions as an alignment mark.

Further, when discarding the supernatant liquid thereafter, as shown in FIG. 9 (a), the small capacity part 3 (small container)
When tilting, it is easy to set the connecting portion 24 of the lid 20 on the upper side so that the convex portion 8 is on the upper side and set the attachment position of the cell pellet P on the upper side to prevent the cell pellet P from flowing out together with the supernatant. It can be done reliably. This recess 8
9B, when the lid 20 is attached, the position of the connecting portion 24 may be on the side opposite to the cell pellet P attachment position. The attachment position of the pellet P is on the lower side, and the cell pellet P easily flows out together with the supernatant.

When centrifuging cell components using the centrifuge container 1 having the above-described structure, the lid 10 attached to the centrifuge container 1 is removed and the cell turbid solution is added to the container 1.
It is put inside, the lid 10 is attached again, and centrifugation is performed at 100 to 600 G for 3 to 30 minutes. After centrifugation, the lid 10 is removed, the container 1 is tilted as shown in FIG. 4 (finally tilted by 90 degrees or more from the vertical direction), and the supernatant liquid U is discarded. At this time, since the inner wall of the container at the boundary A between the large-capacity part 2 and the small-capacity part 3 has a taper with a smooth surface without steps,
The whole amount of the supernatant liquid U flows out smoothly, and only the cell pellet P remains in the bottom portion (tip portion) of the small volume portion 3.
On the other hand, 5 to 100 ml of the washing solution is added and pipetting is carried out to prepare a cell suspension, which is then centrifuged again. These operations are repeated 1 to 4 times if necessary.

After discarding the final supernatant, in the state where only the cell pellet P remains at the bottom (tip) of the small volume part 3, the small volume part 3 is broken and separated from the large volume part 2 as shown in FIG. To do.

The lid 20 shown in FIG. 6 is attached to the small container 3 in order to extract DNA or RNA from the cell pellet in the small volume part 3 (small container) after separation or to freeze and store the DNA or RNA as it is. . The mounting method is the fixing wheel 21 of the lid 20.
Is inserted from the tip of the small container 3, and the fixing wheel 21 is fitted into the circumferential groove 7 formed in the lower root of the annular flange 6. At this time, the concave portion 8 formed at one position in the circumferential direction of the circumferential groove 7
And the convex portion 22 formed on the fixing wheel 21 of the lid 20 are fitted and locked to each other.

When setting the small container 3 in the centrifuge 30, as shown in FIG. 8, the connecting portion 24 of the lid 20 is bent and the fitting portion 23 is fitted into the opening portion of the small container 3 to 3, the concave portion 8 of the circumferential groove 7 and the convex portion 2 of the fixing wheel 21 of the lid 20.
Set so that the locking part of 2 is located on the outermost side.

When the supernatant is discarded after centrifugation,
As shown in FIG. 9A, the fitting portion 23 of the lid 20 is removed from the opening, the small container 3 is tilted, and the supernatant liquid is discarded.
At this time, since the cell pellet P is attached to the upper side, the cell pellet P does not flow out together with the supernatant.

For the cell components remaining in the form of pellets in the small container 3, a freezing solution is put and stored, or at the time of gene extraction, only the cells are frozen as they are, or D
NA or RNA may be directly extracted.

FIG. 10 is a partially cutaway front view showing the centrifuge container of the second embodiment of the present invention. The centrifuge container 41 of the present embodiment is made of transparent synthetic resin, and the upper end of the small capacity part 43 is connected to the lower end of the large capacity part 42 with a screw. The dimensions and capacities of the large-capacity portion 42 and the small-capacity portion 43 are the same as those in the first embodiment.

The connecting portion between the large-capacity portion 42 and the small-capacity portion 43 is
As shown in a partially enlarged view in the figure, a female screw 42 a is provided at the lower end of the large capacity portion 42, and a male screw 43 is provided at the upper end of the small capacity portion 43.
By providing a, the large capacity part 42 and the small capacity part 43 are separably connected. As for the relationship between the female screw and the male screw, contrary to the illustrated case, a male screw may be provided at the lower end of the large capacity portion 42 and a female screw may be provided at the upper end of the small capacity portion 43.
If necessary, an O-ring may be attached to the contact portion between the lower end of the large capacity portion 42 and the upper end of the small capacity portion 43.

The centrifuge container 41 includes a large capacity portion 42.
Since the small-capacity part 43 and the small-capacity part 43 are connected by a screw, the connection is reliable, and the large-capacity part 42 and the small-capacity part 43 are connected during the centrifugation operation.
Since it does not come off, and only needs to be loosened when separating, it is possible to easily separate, and the small volume portion 43 after separation can be used as an independent small container for centrifugation. At this time, a male screw 43a provided on the upper end of the small-capacity portion 43 can be used to attach a threaded lid (not shown).

The method of using the centrifuge container 41 having the above-described structure is basically the same as that of the first embodiment. For example, when centrifuging a cell component, the large volume portion 42 and the small volume portion are used. The cell suspension is put in a centrifuge container 41 to which 43 is connected, and centrifugation is performed. After centrifugation, the supernatant is discarded, a washing solution is added to the cell pellet remaining at the bottom of the small volume portion 43 to pipet it into a cell suspension, and centrifugation is performed again. These operations are repeated 1 to 4 times if necessary.

After discarding the final supernatant, the small volume portion 43 is separated from the large volume portion 42 in a state where only the cell pellet remains at the bottom of the small volume portion 43. Small capacity part 4 after separation
When centrifuging and washing the cell pellet in 3 (small container) again, a lid with a screw (not shown) is attached to the upper end of the small volume portion 43.

FIG. 11 is a partially cutaway front view showing a centrifuge container of a third embodiment of the present invention, FIG. 12 is a partially enlarged sectional view of FIG. 11, and FIG. Is a perspective view, and FIG. 6B is a view showing a connected state. The centrifuge container 51 of this embodiment is made of a transparent synthetic resin, and the upper end of the small capacity part 53 is connected to the lower end of the large capacity part 52 by using a locking tool 54. Large capacity section 52 and small capacity section 53
The dimensions and capacity of are the same as those in the first embodiment.

The connecting portion between the large capacity portion 52 and the small capacity portion 53 is
Annular flanges 52a and 53a are formed on the lower end of the large capacity portion 52 and the upper end of the small capacity portion 53, respectively, and both flanges 52a and 53a are abutted to each other, and then, FIG.
The large-capacity part 52 and the small-capacity part 53 are connected by fitting the locking tool 54 shown in FIG.

As shown in FIG. 12, the inner wall of the container at the connecting portion of the large-capacity part 52 and the small-capacity part 53 has a tapered surface with no step so that the container inner diameter increases from the container bottom to the container upper part. Is attached. Then, a lid for retrofitting (see FIG. 6) is attached to the lower root portion of the flange 53a of the small capacity portion 53.
A peripheral groove 53b for mounting the reference groove) is formed, and a concave portion 53c is formed at one position in the peripheral groove 53b in the circumferential direction.

As shown in FIG. 13A, the locking member 54 is made of a synthetic resin having a U-shaped cross section, one of which is opened and the other is formed in an arc shape. Large capacity part 52 and small capacity part 5
3, the flanges 52a, 53a of the large-capacity portion 52 and the small-capacity portion 53 are abutted to each other, and the locking tool 54 is attached to the peripheral portions of the both flanges 52a, 53a, as shown in FIG.
Is fitted to connect the large-capacity portion 52 and the small-capacity portion 53. Since the locking tool 54 is made of synthetic resin and has elasticity, the inner dimension of the opening 54a of the locking tool 54 is set to the flanges 52a, 5a.
If formed to be slightly smaller than the outer diameter of 3a, the locking device 5
4 is fitted tightly into the peripheral portions of the flanges 52a and 53a, and the large capacity portion 52 and the small capacity portion 53 are fitted.
Does not come off. If necessary, both flanges 52
An O-ring may be attached between a and 53a.

The centrifuge container 51 includes a large capacity portion 52.
Since the stoppers 54 are fitted to the peripheral portions of the flanges 52a and 53a of the small capacity portion 53 to connect, the connection is reliable, and the large capacity portion 52 and the small capacity portion 53 can be connected during the centrifugal separation operation.
It does not come off, and when separating, it can be easily separated by removing the locking tool 53, and the small volume portion 53 after separation can be used as an independent small container for centrifugation. .

The flange 53a of the small-capacity portion 53 serves as a locking member for the body when the small-capacity portion 53 separated from the large-capacity portion 52 is set in the centrifuge as a small container for centrifugation. The function is the same as in the case of the first embodiment. The peripheral groove 53b formed in the lower root of the flange 53a of the small capacity portion 53 is used as a small container for centrifugal separation after being separated from the large capacity portion 52, and when a lid for retrofitting is attached, The fact that the fixing wheel of the lid is fitted into the groove is the same as in the case of the first embodiment. further,
A recess 53c formed at one location in the circumferential direction of the circumferential groove 53b serves as a recess for engaging with a protrusion formed on the fixing wheel of the lid, and the small capacity portion 53 (small container) is centrifuged. It also functions as an alignment mark when it is set in the container as in the case of the first embodiment.

[0058]

According to the present invention, the following effects can be obtained.

(1) Since the large-capacity part and the small-capacity part are formed so as to be separable, it is not necessary to transfer the treatment solution from the centrifuge container to the microtube, which is required in the related art. To make the cell pellet loose. In addition, the loss and damage of the cells due to the residual adhesion of the cells to the inner wall of the container or pipette, which occurs when the processing solution is transferred to the microtube, is eliminated.

(2) Since the cell component can be stored in the small container after separation, it can be stored in a smaller space as compared with the conventional storage in a centrifuge container.

(3) By tilting the inner wall of the container near the boundary between the large-capacity part and the small-capacity part so that the container inner diameter increases from the container bottom to the container top, the container is tilted after centrifugation. When discarding the supernatant liquid, the supernatant liquid does not flow out smoothly and remain in the container.

(4) By forming a concave portion or a convex portion on the outer periphery of the lower root portion of the annular flange of the small-capacity portion, the concave portion or the convex portion functions as an alignment mark, and centrifugal separation is performed. When discarding the supernatant later, tilt the small volume (small container) so that the recess or protrusion is on the upper side so that part of the cell pellet, which is the precipitate, flows out together with the supernatant. Can be prevented.

(5) The attachment position of the cell pellet in the small container after separation can be easily identified by visual observation, and the operation with a micropipette during the gene extraction process can be reliably performed.
Furthermore, when repeatedly centrifuging using a small container after separation, the precipitation part can always be in the same position,
It is possible to reduce unevenness in processing of the precipitate.

[Brief description of drawings]

FIG. 1 is a front view showing a centrifugation container according to a first embodiment of the present invention.

FIG. 2 is a vertical sectional view of a portion A of FIG.

3 is a partially cutaway front view showing a lid of the centrifuge container of FIG. 1. FIG.

FIG. 4 is a diagram showing a state at the time of discarding a supernatant liquid.

5 is a diagram showing a state of breakage separation of the centrifuge container of FIG. 1. FIG.

FIG. 6 is a perspective view showing a lid to be additionally attached to the opening of the small container after the fracture separation.

FIG. 7 is a perspective view showing a state in which the lid of FIG. 6 is attached to the small container after fracture separation.

FIG. 8 is a view showing a state in which the small container after fracture separation is set in a centrifuge.

FIG. 9 is a diagram showing a state of discarding a supernatant from a small container after fracture separation, where (a) is the cell pellet attachment position on the upper side and (b) is the cell pellet attachment position on the lower side. Shows when it is on the side.

FIG. 10 is a partially cutaway front view showing a centrifuge container according to a second embodiment of the present invention.

FIG. 11 is a partially cutaway front view showing a centrifugation container according to a third embodiment of the present invention.

12 is a partially enlarged sectional view of FIG.

13 (a) is a perspective view showing a connection locking tool used in the centrifugation container of FIG. 11, and FIG. 13 (b) is a view showing a connected state.

[Explanation of symbols]

 1,41,51 Centrifuge container 2,42,52 Large capacity part 3,43,53 Small capacity part (small container) 4,7,53b Circular groove 5,6,52a, 53a Annular flange 8,53c Recess 10 Lid 11 Screw 20 Lid 21 Fixing Ring 22 Convex portion 23 Fitting portion 24 Connecting portion 30 Centrifuge 42a Female screw 43a Male screw 54 Locking device 54a Opening U Supernatant P Cell pellet

Claims (6)

[Claims] 1. A centrifuge container characterized in that a small volume portion having an internal volume of 0.5 to 5 ml is formed so as to be separable below a large volume portion having an internal volume of 5 to 100 ml. 2. The large-capacity part and the small-capacity part are integrally molded, and an annular shape is provided on the outer periphery so that the container wall thickness at the boundary between the large-capacity part and the small-capacity part is thinner than other parts. The container for centrifuging according to claim 1, wherein a groove is provided to form a large-capacity portion and a small-capacity portion so that the large-capacity portion and the small-capacity portion can be separated by breaking, and annular flanges are formed immediately above and below the annular groove, respectively. 3. The centrifuge container according to claim 1, wherein an inner wall of the container near the boundary between the large-capacity part and the small-capacity part is tapered so that the container inner diameter increases from the container bottom to the container upper part. . 4. The large-capacity portion and the small-capacity portion are separately molded, and screws are provided on the lower end portion of the large-capacity portion and the upper end portion of the small-capacity portion to be screwed with each other. The centrifuge container according to claim 1, wherein a large-capacity part and the small-capacity part are connected. 5. The large-capacity portion and the small-capacity portion are separately molded, and annular flanges are formed at the lower end portion of the large-capacity portion and the upper end portion of the small-capacity portion, respectively, and both flanges are butted. The centrifuge container according to claim 1, wherein the large capacity portion and the small capacity portion are connected by fitting a locking tool to the peripheral edge portion. 6. The centrifuge container according to claim 2, wherein a concave portion or a convex portion is formed at one or more locations on the outer circumference of the lower root portion of the annular flange of the small capacity portion.